FastQCFastQC Report
Thu 26 May 2016
SRR938906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827514
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.31934091886573784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.2580007595017056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29040.1589043914301067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.033.6056141
GTATCAA80250.031.8995781
ATCAACG97100.025.9328383
TATCAAC98550.025.6953662
TCAACGC99000.025.4829484
CAACGCA99400.025.3804045
AACGCAG102050.024.7677156
GTACATG66250.023.47061
TACATGG67950.022.499392
ACGCAGA112050.022.0081487
CGCAGAG112100.021.6189178
GAGTACT51150.021.00631112-13
ACATGGG70900.020.8956433
GCAGAGT115250.020.2887639
CATGGGG40500.019.8678824
AGTACTT55700.019.67276412-13
GTACTTT55600.019.2399214-15
AGAGTAC94600.019.13858610-11
TAATACC4850.018.5425534
CGCGGAA2101.8189894E-1218.117782-83
Produced by FastQC (version 0.11.5)