FastQCFastQC Report
Thu 26 May 2016
SRR938906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56940.3115708005520067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50830.2781374041457411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.17843912550054336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54100.038.5650371
GTATCAA79150.028.646591
GTACATG67150.024.8987771
ATCAACG95400.023.6773223
TACATGG69600.023.5907042
TATCAAC97250.023.3732952
TCAACGC97200.023.1900314
ACATGGG68900.023.0727633
CAACGCA97850.023.0359865
AACGCAG100400.022.4502946
GAGTACT55600.020.22830412-13
CATGGGG36700.019.6540264
ACGCAGA113700.019.4903037
CGCAGAG112750.019.2757428
GTATAGG4500.019.0535931
GTACTTT59800.018.76790814-15
CATGGGA36600.018.1518544
GCAGAGT120100.017.7009749
TATCCCG1350.00945490717.5756055
AGTACTT59500.017.4269112-13
Produced by FastQC (version 0.11.5)