##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938906_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827514 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08951231016561 33.0 31.0 34.0 30.0 34.0 2 32.334508518129 34.0 31.0 34.0 30.0 34.0 3 32.42351741217851 34.0 31.0 34.0 30.0 34.0 4 36.01827619378018 37.0 35.0 37.0 35.0 37.0 5 35.9278489795427 37.0 35.0 37.0 35.0 37.0 6 35.96645990126478 37.0 35.0 37.0 35.0 37.0 7 35.90845870400993 37.0 35.0 37.0 35.0 37.0 8 35.869697304644454 37.0 35.0 37.0 35.0 37.0 9 37.54267053494529 39.0 37.0 39.0 35.0 39.0 10-11 37.45237519384256 39.0 37.0 39.0 34.0 39.0 12-13 37.368531513301676 39.0 37.0 39.0 34.0 39.0 14-15 38.609623784003844 40.0 38.0 41.0 34.0 41.0 16-17 38.59193937775579 40.0 38.0 41.0 34.0 41.0 18-19 38.693628338825306 40.0 38.0 41.0 34.0 41.0 20-21 38.69346746454473 40.0 38.0 41.0 34.0 41.0 22-23 38.62907178823254 40.0 38.0 41.0 34.0 41.0 24-25 38.5602827119245 40.0 38.0 41.0 34.0 41.0 26-27 38.403377210790175 40.0 38.0 41.0 34.0 41.0 28-29 38.208491699653194 40.0 38.0 41.0 33.0 41.0 30-31 38.03640464587412 40.0 37.5 41.0 33.0 41.0 32-33 37.88350650118139 40.0 37.0 41.0 33.0 41.0 34-35 37.865342208048745 40.0 37.0 41.0 33.0 41.0 36-37 37.7649443451596 40.0 37.0 41.0 33.0 41.0 38-39 37.638617816334104 40.0 37.0 41.0 33.0 41.0 40-41 37.474011143006294 40.0 37.0 41.0 32.0 41.0 42-43 37.300906586762125 40.0 37.0 41.0 31.5 41.0 44-45 37.04857199452371 40.0 36.0 41.0 31.0 41.0 46-47 36.73585428073328 39.5 36.0 41.0 30.5 41.0 48-49 36.443274032374035 39.0 35.5 41.0 30.0 41.0 50-51 36.163469335939425 39.0 35.0 41.0 29.5 41.0 52-53 35.91190354766093 39.0 35.0 40.0 29.0 41.0 54-55 35.64074420223319 38.5 35.0 40.0 28.0 41.0 56-57 35.346332777751634 38.0 34.5 40.0 28.0 41.0 58-59 35.01167022523494 38.0 34.0 40.0 27.0 41.0 60-61 34.57902073527207 37.5 34.0 40.0 26.0 41.0 62-63 34.23219056051007 37.0 33.0 40.0 26.0 41.0 64-65 33.93745601948877 36.5 33.0 39.0 26.0 41.0 66-67 33.45803342683011 36.0 33.0 39.0 24.5 40.0 68-69 33.02434755629778 35.5 32.0 39.0 23.5 40.0 70-71 32.54405410847741 35.0 32.0 38.0 22.5 40.0 72-73 32.00205798697028 35.0 31.0 37.0 21.5 39.0 74-75 31.510249716281244 35.0 31.0 37.0 20.0 39.0 76-77 29.554949784242417 32.5 28.5 35.0 17.5 37.0 78-79 30.541023215143632 34.0 30.0 36.0 18.0 37.5 80-81 30.539344705430437 34.0 30.0 35.0 18.0 37.0 82-83 30.1951848248495 34.0 30.0 35.0 15.5 37.0 84-85 29.76321877698338 34.0 30.0 35.0 9.0 36.0 86-87 29.1412799026437 34.0 29.0 35.0 4.5 36.0 88-89 28.534113008163 33.5 29.0 35.0 2.0 35.0 90-91 28.02172897170692 33.0 27.0 35.0 2.0 35.0 92-93 27.601115778046022 33.0 27.0 35.0 2.0 35.0 94-95 27.128608043495152 33.0 26.0 34.5 2.0 35.0 96-97 26.408664174392097 32.0 25.0 34.0 2.0 35.0 98-99 25.36266616835767 32.0 21.5 34.0 2.0 35.0 100-101 20.857842402301706 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15704622661603906 1101 2 0.10056926289598067 1101 3 0.09761930252167161 1101 4 0.05058811241119088 1101 5 0.14721365116949414 1101 6 0.09236567373486793 1101 7 0.09368735309977438 1101 8 0.10957434589946757 1101 9 0.21873766611418688 1101 10-11 0.20229465538901792 1101 12-13 0.12762424814562223 1101 14-15 0.06832316240507907 1101 16-17 0.13566355967005705 1101 18-19 0.15045620412713845 1101 20-21 0.2113070740095182 1101 22-23 0.29740720349602867 1101 24-25 0.22396971072528515 1101 26-27 0.18932675313186564 1101 28-29 0.23718897288950558 1101 30-31 0.16169216596488667 1101 32-33 0.2118697271599288 1101 34-35 0.3093358638791699 1101 36-37 0.28847091896366095 1101 38-39 0.2799085140637132 1101 40-41 0.46170597716405126 1101 42-43 0.3399539700076417 1101 44-45 0.2897989814915647 1101 46-47 0.2346366077336981 1101 48-49 0.04127402764687105 1101 50-51 0.08016228865311348 1101 52-53 0.15891858713178664 1101 54-55 0.10061849500090148 1101 56-57 0.011101885749468465 1101 58-59 0.08069243667689108 1101 60-61 0.31694033772505037 1101 62-63 0.268196854467341 1101 64-65 0.06931746789138771 1101 66-67 0.0994486977948057 1101 68-69 -0.07378458603047733 1101 70-71 -0.036012635978885044 1101 72-73 -0.07690425167287529 1101 74-75 -0.16332260343924432 1101 76-77 -0.16531705481091308 1101 78-79 -0.07496901897819086 1101 80-81 0.16454718957454517 1101 82-83 0.19305577465353707 1101 84-85 0.14318626014379632 1101 86-87 0.34307740615220084 1101 88-89 0.353772753890631 1101 90-91 0.23133118394146024 1101 92-93 0.4947921159300037 1101 94-95 0.4540493755632724 1101 96-97 0.2097099102179385 1101 98-99 0.14121636192810172 1101 100-101 0.649704788857747 1102 1 0.23914351820868518 1102 2 0.18564846709234217 1102 3 0.19248409113632903 1102 4 0.1818784720692932 1102 5 0.1890818984370597 1102 6 0.19828561271211242 1102 7 0.18435421481635217 1102 8 0.2425352558073044 1102 9 0.2894807017874257 1102 10-11 0.2639593802641542 1102 12-13 0.25672225550179917 1102 14-15 0.37155938003898115 1102 16-17 0.2999195313320939 1102 18-19 0.21832508356539648 1102 20-21 0.3165419437092112 1102 22-23 0.33885944587340333 1102 24-25 0.19965269018235432 1102 26-27 0.3067374394289146 1102 28-29 0.22431959711888538 1102 30-31 0.21397751527837983 1102 32-33 0.2803551036410923 1102 34-35 0.3762884876487931 1102 36-37 0.33084790690539023 1102 38-39 0.35813637241568586 1102 40-41 0.39867241987162316 1102 42-43 0.2537416912673862 1102 44-45 0.33116959025627324 1102 46-47 0.5048445187491382 1102 48-49 0.3127168919548282 1102 50-51 0.39432696128272937 1102 52-53 0.42922511155133236 1102 54-55 0.5133658775007461 1102 56-57 0.5149896049193927 1102 58-59 0.5127197192833108 1102 60-61 0.3456662264212156 1102 62-63 0.430451169946636 1102 64-65 0.5178131889839435 1102 66-67 0.546908651609769 1102 68-69 0.4652121172777086 1102 70-71 0.4818638190586242 1102 72-73 0.6477018171658102 1102 74-75 0.49825447958932045 1102 76-77 0.3228917861746474 1102 78-79 0.4455432221453002 1102 80-81 0.7195911019274739 1102 82-83 0.7505767773494014 1102 84-85 0.765577249882778 1102 86-87 0.7439030158573239 1102 88-89 0.8164498661766899 1102 90-91 0.934188099157442 1102 92-93 0.6862895506753581 1102 94-95 0.8234388606315051 1102 96-97 0.6955944789834838 1102 98-99 0.8008061562897666 1102 100-101 1.513022039280937 1103 1 0.03639939264346026 1103 2 0.08771922168603652 1103 3 0.10989110647912526 1103 4 0.06544900449303981 1103 5 0.15068080326990696 1103 6 -0.020990243949071896 1103 7 0.09084419103109553 1103 8 0.03150615154425651 1103 9 0.10978115396861199 1103 10-11 0.10666518151353443 1103 12-13 0.11680501380703134 1103 14-15 0.20860540189005405 1103 16-17 0.1902201795455838 1103 18-19 0.1748799138014121 1103 20-21 0.2411755266570168 1103 22-23 0.26916849080401306 1103 24-25 0.1637413686632314 1103 26-27 0.14722154120889286 1103 28-29 0.25939010340498925 1103 30-31 0.2606800885291136 1103 32-33 0.35410327400622776 1103 34-35 0.22397334611346764 1103 36-37 0.1699953950781108 1103 38-39 0.16791560736693611 1103 40-41 0.13348040622776836 1103 42-43 0.10700742612716141 1103 44-45 0.25469216010912277 1103 46-47 0.37140026380259883 1103 48-49 0.4618056684518592 1103 50-51 0.4415774448290861 1103 52-53 0.5088433059631043 1103 54-55 0.5614604434852168 1103 56-57 0.484833138467053 1103 58-59 0.41494958676546645 1103 60-61 0.3072108956493693 1103 62-63 0.43972921563523926 1103 64-65 0.4647911876247903 1103 66-67 0.4288584869426302 1103 68-69 0.2982956727809878 1103 70-71 0.2922246165180482 1103 72-73 0.5148200115775836 1103 74-75 0.5218051474060488 1103 76-77 0.3432031834929248 1103 78-79 0.5880069234966143 1103 80-81 0.5210997338833536 1103 82-83 0.6414563743069799 1103 84-85 0.5123909081069833 1103 86-87 0.36475546234050427 1103 88-89 0.6494711814227756 1103 90-91 0.8306560870818522 1103 92-93 0.9273643631377873 1103 94-95 0.8179484858177375 1103 96-97 0.7009673977738338 1103 98-99 0.9027702366368757 1103 100-101 1.5548626846192874 1104 1 0.07294938285198782 1104 2 0.05815655309897494 1104 3 0.1315567939984703 1104 4 0.0584143047754111 1104 5 0.11945705863152511 1104 6 0.09463978997364819 1104 7 0.03465629581881302 1104 8 0.01836852133345701 1104 9 0.1148000736216801 1104 10-11 0.12542485454149954 1104 12-13 0.16720978067520775 1104 14-15 0.14183987292939548 1104 16-17 0.13075798410778106 1104 18-19 0.131635082751707 1104 20-21 0.11007733397345021 1104 22-23 0.1124455389591077 1104 24-25 0.1730470584567243 1104 26-27 0.1746056070075923 1104 28-29 0.1408900623439706 1104 30-31 0.13134704578143186 1104 32-33 0.20424556622169376 1104 34-35 0.2838028797234884 1104 36-37 0.2856317460803197 1104 38-39 0.4785129819887075 1104 40-41 0.40944549804652297 1104 42-43 0.3282670833624266 1104 44-45 0.26396673828613615 1104 46-47 0.3995643309910548 1104 48-49 0.2621246809659894 1104 50-51 0.3140243971540855 1104 52-53 0.3051722931457519 1104 54-55 0.2817017875070462 1104 56-57 0.37522565020103116 1104 58-59 0.303074740744286 1104 60-61 0.40957237788889955 1104 62-63 0.3586147564719937 1104 64-65 0.3812874510722679 1104 66-67 0.4489605393617637 1104 68-69 0.36466275195294173 1104 70-71 -0.03612691000421364 1104 72-73 0.0717365819087874 1104 74-75 0.2562108049824374 1104 76-77 0.08815030953761038 1104 78-79 0.303305152819231 1104 80-81 0.3693773821610016 1104 82-83 0.41519185292050764 1104 84-85 0.3353705481597977 1104 86-87 0.21105997084013595 1104 88-89 0.29466214673568913 1104 90-91 0.41307739206413174 1104 92-93 0.43277099354441617 1104 94-95 0.6966430297561814 1104 96-97 0.7315886509560627 1104 98-99 0.6848425229774548 1104 100-101 1.2868992794607585 1105 1 -0.0587896451165264 1105 2 0.016331486191454303 1105 3 -0.017026025112464538 1105 4 -0.006348721060945195 1105 5 0.020625487905739703 1105 6 -0.026985991847986668 1105 7 -0.07627384520213099 1105 8 0.039678366394269915 1105 9 -0.09235536035487968 1105 10-11 0.004753638533543381 1105 12-13 -0.06018988272042236 1105 14-15 -0.03963929844145753 1105 16-17 0.04210675213332138 1105 18-19 -0.04593112453883208 1105 20-21 0.08627395756512612 1105 22-23 -0.09189910048061023 1105 24-25 -0.060821411659155444 1105 26-27 -0.06432904880580281 1105 28-29 -0.09279563826322601 1105 30-31 -0.03150536776318802 1105 32-33 -0.08774900590948675 1105 34-35 0.08911325165404094 1105 36-37 0.05080053564952891 1105 38-39 0.0809377858315159 1105 40-41 0.04850116732532683 1105 42-43 0.04121638788202375 1105 44-45 -0.09550356020346129 1105 46-47 0.1623890437730111 1105 48-49 0.2689567131655082 1105 50-51 0.053658358412505436 1105 52-53 0.2758728756637936 1105 54-55 0.25733390780609966 1105 56-57 0.03512398408344097 1105 58-59 -0.008051711694925245 1105 60-61 0.027614797689331283 1105 62-63 -0.03848708979308668 1105 64-65 -0.05300390295828805 1105 66-67 0.1385798173651267 1105 68-69 -0.05571837722639117 1105 70-71 0.02307946006069983 1105 72-73 0.045511239597693987 1105 74-75 0.07548245383794239 1105 76-77 0.03247162234400491 1105 78-79 0.2617399733565442 1105 80-81 0.26931161598505327 1105 82-83 0.3651953694983945 1105 84-85 0.3362188454574593 1105 86-87 0.1406040989570876 1105 88-89 0.3551748946390063 1105 90-91 0.47622633949684356 1105 92-93 0.5928800136283954 1105 94-95 0.5517897882733962 1105 96-97 0.4070206964815526 1105 98-99 0.6572936826461344 1105 100-101 1.0516878776047456 1106 1 0.028502362388259428 1106 2 -0.0656284358885415 1106 3 -0.08845114781418317 1106 4 -0.029165144181177993 1106 5 -0.024957528653189343 1106 6 -0.11538477538475433 1106 7 -0.02279235986907935 1106 8 0.004683692908670878 1106 9 -0.02531989214467245 1106 10-11 -0.051960895577501276 1106 12-13 0.09396783162613076 1106 14-15 -0.021612250518927567 1106 16-17 -0.0016381372293068353 1106 18-19 -0.13695850115157526 1106 20-21 -0.10104200532692431 1106 22-23 -0.03352966384075273 1106 24-25 0.024543177574052777 1106 26-27 -0.0670274343860271 1106 28-29 0.04450525192391552 1106 30-31 -0.03819647263888015 1106 32-33 -0.1340895264780002 1106 34-35 0.031010604986093426 1106 36-37 -0.21523827702986864 1106 38-39 -0.08883611740481001 1106 40-41 0.030675295135672798 1106 42-43 0.05852446468534822 1106 44-45 0.08719820512907006 1106 46-47 0.03950363285146352 1106 48-49 0.19845576083053373 1106 50-51 0.180879106377688 1106 52-53 0.37863436852391175 1106 54-55 0.3613748783273536 1106 56-57 0.5037433552638078 1106 58-59 0.45828168415763315 1106 60-61 0.4930566755440893 1106 62-63 0.37017108044862823 1106 64-65 0.34469684411553914 1106 66-67 0.38404516159538105 1106 68-69 0.32942126658733883 1106 70-71 0.42624104502835536 1106 72-73 0.45090695845429707 1106 74-75 0.5103333949999183 1106 76-77 0.30463468858405207 1106 78-79 0.46648998001081665 1106 80-81 0.5588808708858473 1106 82-83 0.5189132827427549 1106 84-85 0.49770539175294815 1106 86-87 0.5419708868862969 1106 88-89 0.5785051824812442 1106 90-91 0.7712429689924285 1106 92-93 0.6914548847366504 1106 94-95 0.8778517021169492 1106 96-97 0.7725077924510444 1106 98-99 0.9698392179995139 1106 100-101 1.3881983947625613 1107 1 -0.11715241670945176 1107 2 -0.0011995738625998342 1107 3 0.008117169346071762 1107 4 -0.023647656979314036 1107 5 -0.07631231819835449 1107 6 0.01874154053409427 1107 7 -0.014905757812776699 1107 8 0.040249914431669254 1107 9 0.0876927246978596 1107 10-11 0.04638594775363458 1107 12-13 0.03471752895711688 1107 14-15 0.08929120881894903 1107 16-17 0.06614374441942772 1107 18-19 0.024414786540710054 1107 20-21 -0.003131189749154828 1107 22-23 -0.019066386657392798 1107 24-25 -0.04462698694269562 1107 26-27 -0.08757328336494652 1107 28-29 0.033044697225328434 1107 30-31 -0.013493149732518361 1107 32-33 0.0024922494670320816 1107 34-35 -0.12088192139373177 1107 36-37 0.1050042883742961 1107 38-39 0.04320562326469002 1107 40-41 -0.12893636668623998 1107 42-43 -0.11593334294621371 1107 44-45 -0.1060167851302154 1107 46-47 -0.38281736223823515 1107 48-49 -0.339269397264097 1107 50-51 -0.18023238029565647 1107 52-53 -0.11393533753376772 1107 54-55 -0.27340993475532116 1107 56-57 -0.15934608112652882 1107 58-59 -0.14253967044754035 1107 60-61 -0.20788881945034632 1107 62-63 -0.21170472999760648 1107 64-65 -0.24672917251435678 1107 66-67 -0.08323954393992494 1107 68-69 -0.3141415625844246 1107 70-71 -0.21255651395369313 1107 72-73 -0.010721062858522146 1107 74-75 -0.17965240874764277 1107 76-77 -0.3133629023549247 1107 78-79 -0.20133922247936553 1107 80-81 0.08914131988074203 1107 82-83 0.1718265872738769 1107 84-85 0.06886333888880714 1107 86-87 0.03234293932024812 1107 88-89 0.2966788438238055 1107 90-91 0.6042477195132641 1107 92-93 0.5763546198628475 1107 94-95 0.6227130889248826 1107 96-97 0.9249186971394963 1107 98-99 0.6536690104638296 1107 100-101 1.0929015947576453 1108 1 0.0022849952107790727 1108 2 -0.005057623573883063 1108 3 0.009813289471281905 1108 4 1.9654939860203058E-4 1108 5 0.02737300163253309 1108 6 0.13245267631100432 1108 7 0.05183407482007851 1108 8 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-1.4869015079541406 2316 72-73 -1.4181413874935984 2316 74-75 -1.7436709216499722 2316 76-77 -1.6524641563271558 2316 78-79 -1.8182731219242427 2316 80-81 -1.8476979053200147 2316 82-83 -2.0180157102247698 2316 84-85 -2.319801464180852 2316 86-87 -2.2176629943022945 2316 88-89 -2.373578543536258 2316 90-91 -2.330890533891978 2316 92-93 -2.789549466425207 2316 94-95 -3.0464070207812064 2316 96-97 -3.096822755147585 2316 98-99 -3.445022296119859 2316 100-101 -4.0947650411420895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 24.0 5 39.0 6 88.0 7 273.0 8 534.0 9 1033.0 10 1722.0 11 2622.0 12 3682.0 13 5236.0 14 6480.0 15 8414.0 16 11035.0 17 15402.0 18 15065.0 19 11214.0 20 10663.0 21 11766.0 22 12217.0 23 13890.0 24 15859.0 25 18716.0 26 22857.0 27 27819.0 28 33240.0 29 40241.0 30 49108.0 31 58977.0 32 73297.0 33 93441.0 34 124966.0 35 177866.0 36 274238.0 37 403925.0 38 266403.0 39 15160.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.90810985417821 19.728957360968277 15.150820961773418 23.2121118230801 2 15.877361267820659 20.72974543560268 39.36473263679512 24.028160659781538 3 19.210796743554358 24.459839979338053 30.07112394214217 26.25823933496542 4 13.601810984758531 17.28916987776838 36.05280178428182 33.05621735319127 5 14.148047910181269 37.027189558649155 34.032392833281264 14.792369697888322 6 32.34722141663484 35.8803270453742 17.086380733608607 14.686070804382348 7 28.611928554309294 32.64724647800236 20.47141636124265 18.269408606445698 8 26.107871129851812 35.64470641538177 19.39782677451445 18.84959568025197 9 26.312301848303214 16.957954904859825 20.208600317152154 36.52114292968481 10-11 25.12328222930166 26.618838487694212 26.891038864818544 21.36684041818558 12-13 26.004889702623345 23.87981706296094 27.55439903606758 22.560894198348137 14-15 22.849444566955935 25.174542392228037 25.843765201836614 26.13224783897941 16-17 22.41808536572353 28.435158729311865 26.56456356381909 22.582192341145515 18-19 22.344753583283083 27.068930798888545 28.62719519522149 21.959120422606887 20-21 23.040069734075907 26.231180718725 28.687030578151525 22.04171896904757 22-23 22.884585289086704 26.101250113542225 28.597920453687358 22.416244143683713 24-25 22.69974402384879 26.35695266903564 28.60246214256088 22.34084116455469 26-27 22.60798002094649 26.505761378572203 28.49526734131722 22.39099125916409 28-29 22.388173223296786 26.50770390815064 28.713569362532926 22.390553506019653 30-31 22.643957857504784 26.358129130611314 28.565499361427598 22.432413650456304 32-33 22.388337380725947 26.46767685500631 28.55091670980359 22.593069054464152 34-35 22.85814033214745 26.446349505313766 28.351139812577102 22.344370349961682 36-37 22.834478914798126 26.273658484932145 28.379209946899486 22.512652653370242 38-39 22.66088140030663 26.449041488043328 28.519352887110415 22.37072422453962 40-41 22.7347370955474 26.340131438546187 28.526678960530134 22.398452505376284 42-43 22.583196671750095 26.424741464738577 28.220411741240937 22.771650122270394 44-45 22.69911710227879 26.581733018514313 28.069827442332578 22.649322436874318 46-47 22.804673251593567 26.67270221126376 27.769934670564872 22.752689866577803 48-49 22.934141500807932 26.74405433844649 27.559317081338584 22.762487079406995 50-51 22.733718430635015 26.913491547348613 27.509468518117746 22.843321503898625 52-53 22.98059002557573 26.863050023146194 27.342718031161457 22.813641920116616 54-55 22.756624573053884 26.81506133468745 27.371035187692133 23.05727890456653 56-57 22.758238787773994 27.197110391493577 27.266494264886614 22.77815655584581 58-59 22.935507726755507 26.82346806193224 27.373641836298333 22.86738237501392 60-61 22.909179059067647 26.72758533136631 27.61726085985825 22.74597474970779 62-63 22.78622762944634 26.69790764940788 27.6633722094605 22.852492511685274 64-65 22.677143595853916 26.69838564274018 27.604182198978666 23.02028856242724 66-67 22.64218052917592 26.948814298135744 27.350131300811004 23.05887387187733 68-69 22.53340319264343 26.80704192789808 27.611892316342292 23.0476625631162 70-71 22.57789066948069 26.74759994933006 27.45856596955371 23.215943411635536 72-73 22.651126065244917 26.67569167732778 27.30310137159004 23.370080885837265 74-75 22.59419079689677 26.968138137382258 27.102719869724663 23.334951195996307 76-77 22.65180808832311 26.796638137991714 27.293515908087336 23.258037865597835 78-79 22.67658907941806 26.885506939077025 27.18148302679188 23.25642095471303 80-81 22.56912942937783 26.99246079646996 27.172842451548934 23.265567322603275 82-83 22.61802098369698 26.853227936967926 27.230379630470686 23.298371448864412 84-85 22.616379409405347 26.804418461363362 27.02531964187415 23.553882487357143 86-87 22.529366057024617 26.64683357298169 27.377258758855298 23.44654161113839 88-89 22.585116073305407 26.648512967484088 27.186749081896355 23.57962187731415 90-91 22.460006664869603 26.739879582166477 27.25239927070735 23.54771448225657 92-93 22.49230375252939 26.612737303243637 27.19213094947563 23.702827994751342 94-95 22.40592958521795 26.581793627846352 27.137986357423255 23.874290429512442 96-97 22.406267322057158 26.48900608532589 27.124925650242325 23.97980094237463 98-99 22.256300088535575 26.39873073475771 27.119354489213215 24.2256146874935 100-101 22.499189472028235 25.805623764543334 27.74905780398219 23.94612895944624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 403.0 1 283.5 2 197.0 3 239.0 4 229.0 5 232.0 6 433.0 7 889.0 8 1447.0 9 1806.5 10 1715.5 11 1537.5 12 1611.0 13 1804.5 14 1921.5 15 2008.5 16 2109.0 17 2224.0 18 2350.5 19 2561.0 20 2916.5 21 3364.0 22 4004.5 23 4907.5 24 6001.5 25 7258.0 26 9162.0 27 11404.0 28 13444.5 29 15807.0 30 18633.0 31 21790.0 32 24769.5 33 27694.5 34 30879.5 35 34259.0 36 39166.5 37 43673.0 38 46325.0 39 48116.0 40 49554.0 41 52659.0 42 55499.0 43 58347.0 44 62949.5 45 66020.5 46 67186.5 47 69103.5 48 74101.0 49 75058.5 50 72013.5 51 70534.5 52 68093.0 53 67460.0 54 66562.0 55 62077.5 56 57064.5 57 51566.0 58 46040.0 59 41215.5 60 35472.0 61 28709.0 62 23000.5 63 18342.0 64 14209.0 65 10675.5 66 7479.5 67 5701.5 68 4084.0 69 2569.0 70 1756.5 71 1103.5 72 681.0 73 430.0 74 299.5 75 234.0 76 144.0 77 60.5 78 30.5 79 19.0 80 10.0 81 5.0 82 4.5 83 5.0 84 3.5 85 0.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3945250214225445 2 0.0 3 0.0 4 0.0 5 6.01910573598889E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011491020041433336 16-17 0.0025991592950861116 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023255635798138893 36-37 0.007140848168605001 38-39 0.012503324187940557 40-41 0.002407642294395556 42-43 3.830340013811112E-4 44-45 4.924722874900001E-4 46-47 3.830340013811112E-4 48-49 2.7359571527222227E-4 50-51 5.745510020716668E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.735957152722223E-5 60-61 0.0027359571527222227 62-63 0.0 64-65 3.556744298538889E-4 66-67 0.001696293434687778 68-69 0.0017236530062150002 70-71 2.735957152722223E-5 72-73 0.0 74-75 0.0 76-77 0.12125762100864891 78-79 8.207871458166668E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14325471651653557 88-89 0.4304481388377873 90-91 0.3295733986169189 92-93 0.0 94-95 0.0 96-97 1.6415742916333335E-4 98-99 0.0 100-101 8.207871458166668E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1827514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.74887503039993 #Duplication Level Percentage of deduplicated Percentage of total 1 77.44251614287151 44.722181867744155 2 12.709973456210811 14.679733375248366 3 4.190085223896647 7.259181238845983 4 1.851816056353689 4.2776117607062885 5 1.0029200374970473 2.8958751955450497 6 0.636698263972861 2.20611650869448 7 0.40036065822472483 1.6184264333235805 8 0.2837394815666353 1.3108508689745668 9 0.21951928717621141 1.1409292693711355 >10 1.146898628557467 11.950633366641474 >50 0.08029588211795834 3.1922448285092115 >100 0.033849763921272806 3.5115521303078863 >500 9.479411665286629E-4 0.39502213950204657 >1k 1.895882333057326E-4 0.24968070652284288 >5k 1.895882333057326E-4 0.5899603100628542 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5694 0.3115708005520067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5083 0.2781374041457411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3261 0.17843912550054336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.471914305444445E-5 2 0.0 0.0 0.0 0.0 5.471914305444445E-5 3 0.0 0.0 0.0 0.0 5.471914305444445E-5 4 0.0 0.0 0.0 0.0 5.471914305444445E-5 5 0.0 0.0 0.0 0.0 5.471914305444445E-5 6 0.0 0.0 0.0 0.0 5.471914305444445E-5 7 0.0 0.0 0.0 0.0 5.471914305444445E-5 8 0.0 0.0 0.0 5.471914305444445E-5 5.471914305444445E-5 9 0.0 0.0 0.0 5.471914305444445E-5 1.094382861088889E-4 10-11 0.0 0.0 0.0 5.471914305444445E-5 1.094382861088889E-4 12-13 0.0 0.0 0.0 5.471914305444445E-5 2.7359571527222227E-4 14-15 0.0 0.0 0.0 1.094382861088889E-4 3.283148583266667E-4 16-17 0.0 0.0 0.0 1.6415742916333335E-4 4.377531444355556E-4 18-19 0.0 0.0 0.0 2.188765722177778E-4 4.377531444355556E-4 20-21 0.0 0.0 0.0 2.7359571527222227E-4 4.924722874900001E-4 22-23 0.0 0.0 0.0 4.924722874900001E-4 4.924722874900001E-4 24-25 0.0 0.0 0.0 5.745510020716668E-4 5.471914305444445E-4 26-27 0.0 0.0 0.0 0.0011217424326161113 6.292701451261111E-4 28-29 0.0 0.0 0.0 0.0030369124395216674 6.566297166533334E-4 30-31 0.0 0.0 0.0 0.008891860746347225 6.839892881805557E-4 32-33 0.0 0.0 0.0 0.019808329785708893 7.660680027622224E-4 34-35 0.0 0.0 0.0 0.03586839827218834 7.660680027622224E-4 36-37 0.0 0.0 0.0 0.06303645279872 7.660680027622224E-4 38-39 0.0 0.0 0.0 0.1020512017965389 7.660680027622224E-4 40-41 0.0 0.0 0.0 0.1431726378019539 7.660680027622224E-4 42-43 0.0 0.0 0.0 0.18396575894904227 7.660680027622224E-4 44-45 0.0 0.0 0.0 0.23521023641952948 7.934275742894445E-4 46-47 0.0 0.0 0.0 0.3038006822382756 8.755062888711112E-4 48-49 0.0 0.0 0.0 0.38005180808464395 9.302254319255557E-4 50-51 0.0 0.0 0.0 0.45726051893446507 9.302254319255557E-4 52-53 0.0 0.0 0.0 0.5400505823758395 0.0010396637180344445 54-55 0.0 0.0 0.0 0.6343863850017017 0.0010670232895616668 56-57 0.0 0.0 0.0 0.7487767535570179 0.001094382861088889 58-59 0.0 0.0 0.0 0.8779412907370341 0.001094382861088889 60-61 0.0 0.0 0.0 1.0194723542473545 0.001094382861088889 62-63 0.0 0.0 0.0 1.1623713963340363 0.001094382861088889 64-65 0.0 0.0 0.0 1.3125754440184862 0.001094382861088889 66-67 0.0 0.0 0.0 1.4901664228016858 0.0011217424326161113 68-69 0.0 0.0 0.0 1.6975246154065031 0.0011491020041433336 70-71 0.0 0.0 0.0 1.9295337819573475 0.0011491020041433336 72-73 0.0 0.0 0.0 2.168492279676107 0.0011764615756705557 74-75 0.0 0.0 0.0 2.417245504001611 0.001203821147197778 76-77 0.0 0.0 0.0 2.688816610980819 0.001203821147197778 78-79 0.0 0.0 0.0 2.995736284373198 0.001203821147197778 80-81 0.0 0.0 0.0 3.334119465021882 0.0012311807187250002 82-83 0.0 0.0 0.0 3.6952384496096884 0.0013132594333066668 84-85 0.0 0.0 0.0 4.0639086759390075 0.0013132594333066668 86-87 0.0 0.0 0.0 4.45077301733393 0.001450057290942778 88-89 0.0 0.0 0.0 4.872985925142023 0.0015321360055244447 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5410 0.0 38.565037 1 GTATCAA 7915 0.0 28.64659 1 GTACATG 6715 0.0 24.898777 1 ATCAACG 9540 0.0 23.677322 3 TACATGG 6960 0.0 23.590704 2 TATCAAC 9725 0.0 23.373295 2 TCAACGC 9720 0.0 23.190031 4 ACATGGG 6890 0.0 23.072763 3 CAACGCA 9785 0.0 23.035986 5 AACGCAG 10040 0.0 22.450294 6 GAGTACT 5560 0.0 20.228304 12-13 CATGGGG 3670 0.0 19.654026 4 ACGCAGA 11370 0.0 19.490303 7 CGCAGAG 11275 0.0 19.275742 8 GTATAGG 450 0.0 19.053593 1 GTACTTT 5980 0.0 18.767908 14-15 CATGGGA 3660 0.0 18.151854 4 GCAGAGT 12010 0.0 17.700974 9 TATCCCG 135 0.009454907 17.575605 5 AGTACTT 5950 0.0 17.42691 12-13 >>END_MODULE