##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938905_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425415 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.776408918350317 33.0 31.0 34.0 30.0 34.0 2 31.848561992407415 33.0 31.0 34.0 30.0 34.0 3 31.88258524029477 34.0 31.0 34.0 30.0 34.0 4 35.40710835301999 37.0 35.0 37.0 33.0 37.0 5 35.460087208960665 37.0 35.0 37.0 33.0 37.0 6 35.55423527614212 37.0 35.0 37.0 33.0 37.0 7 35.4874651810585 37.0 35.0 37.0 33.0 37.0 8 35.474066499770814 37.0 35.0 37.0 33.0 37.0 9 37.06771740535712 39.0 37.0 39.0 33.0 39.0 10-11 36.95473831435187 39.0 37.0 39.0 33.0 39.0 12-13 36.85393204282877 39.0 37.0 39.0 33.0 39.0 14-15 38.01194950812736 40.0 38.0 41.0 33.0 41.0 16-17 37.94663446281866 40.0 38.0 41.0 33.0 41.0 18-19 37.91642278716077 40.0 38.0 41.0 33.0 41.0 20-21 38.0201379829108 40.0 38.0 41.0 33.0 41.0 22-23 37.96177144670499 40.0 38.0 41.0 33.0 41.0 24-25 37.87575896477557 40.0 38.0 41.0 32.5 41.0 26-27 37.71914836101219 40.0 37.5 41.0 32.0 41.0 28-29 37.59189379782095 40.0 37.0 41.0 32.0 41.0 30-31 37.423658075056125 40.0 37.0 41.0 32.0 41.0 32-33 37.209990244819764 40.0 36.5 41.0 31.0 41.0 34-35 36.892024258665074 40.0 36.0 41.0 30.5 41.0 36-37 36.60657710706016 39.5 36.0 41.0 30.0 41.0 38-39 36.34408753805108 39.0 35.5 41.0 30.0 41.0 40-41 36.19283053018817 39.0 35.0 41.0 30.0 41.0 42-43 35.99324659450184 39.0 35.0 41.0 29.0 41.0 44-45 35.91853014115628 39.0 35.0 41.0 28.5 41.0 46-47 35.66088525322333 39.0 35.0 41.0 26.5 41.0 48-49 35.40349658568692 39.0 35.0 41.0 25.5 41.0 50-51 34.28506164568715 38.0 34.0 40.0 23.5 40.5 52-53 34.3645675399316 38.0 34.0 39.5 24.0 40.5 54-55 34.8746776676892 39.0 35.0 40.0 24.0 41.0 56-57 34.8112278598545 39.0 35.0 41.0 23.5 41.0 58-59 34.621349740841296 38.5 34.0 40.0 23.0 41.0 60-61 34.2872536229329 38.0 34.0 40.0 22.5 41.0 62-63 33.90989034237157 38.0 34.0 40.0 22.0 41.0 64-65 33.50859278586792 37.0 33.0 40.0 20.5 41.0 66-67 33.08581267703302 36.5 33.0 39.5 18.5 41.0 68-69 32.659882702772585 36.0 32.0 39.0 18.0 41.0 70-71 32.18532491802122 35.5 32.0 39.0 13.5 40.0 72-73 31.725166014362447 35.0 31.0 38.0 10.0 40.0 74-75 31.25976164451183 35.0 31.0 37.0 7.5 39.0 76-77 30.807084846561594 35.0 31.0 37.0 6.0 39.0 78-79 30.387690843059133 35.0 30.5 36.0 2.0 39.0 80-81 29.941939047753372 34.0 30.0 36.0 2.0 37.5 82-83 29.460051949273065 34.0 29.5 35.0 2.0 37.0 84-85 29.034005618043558 34.0 29.0 35.0 2.0 37.0 86-87 28.58347378442227 34.0 29.0 35.0 2.0 36.0 88-89 28.070553459563016 34.0 28.0 35.0 2.0 36.0 90-91 27.563406320886664 34.0 26.5 35.0 2.0 35.5 92-93 27.094503014703292 33.5 25.5 35.0 2.0 35.0 94-95 26.58193293607419 33.0 25.0 35.0 2.0 35.0 96-97 25.919798314586934 33.0 23.5 35.0 2.0 35.0 98-99 25.077793448750043 32.5 17.5 35.0 2.0 35.0 100-101 22.009517765005935 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3269086183324177 1101 2 0.3427895789651352 1101 3 0.5858425574830584 1101 4 0.46697428889793 1101 5 0.4004564225051723 1101 6 0.5312871028144954 1101 7 0.22776990405654374 1101 8 0.3551138661515836 1101 9 0.5943572245230868 1101 10-11 0.3268137390214676 1101 12-13 0.32430335631324425 1101 14-15 0.5636801328976091 1101 16-17 0.5758021987856665 1101 18-19 0.7959929653974243 1101 20-21 0.5898568899425598 1101 22-23 0.6029127070692084 1101 24-25 0.6534321307210789 1101 26-27 0.40745440425820334 1101 28-29 0.4756604420646866 1101 30-31 0.7073898027344967 1101 32-33 0.5051224416798163 1101 34-35 0.5388243285719554 1101 36-37 0.3267136815841525 1101 38-39 0.5728446199982997 1101 40-41 0.7727552445096109 1101 42-43 0.5633593521012372 1101 44-45 0.7484527815448914 1101 46-47 0.7642681289867568 1101 48-49 0.6892231456126439 1101 50-51 0.5221334992748652 1101 52-53 0.23770243864545648 1101 54-55 0.7993013357126699 1101 56-57 0.7576828318955293 1101 58-59 0.8147511394047129 1101 60-61 0.8407989134341491 1101 62-63 0.4261768746965373 1101 64-65 0.5626742813020655 1101 66-67 0.44360253705383457 1101 68-69 0.6681459077587064 1101 70-71 0.5032962566504153 1101 72-73 0.4027630676780092 1101 74-75 0.575392478568844 1101 76-77 0.2803224169205478 1101 78-79 0.13365023247629537 1101 80-81 -0.2108294428000157 1101 82-83 0.02941609077364049 1101 84-85 0.03649811511554901 1101 86-87 -0.06450900101551227 1101 88-89 -0.3499976101769846 1101 90-91 -0.08093267844743934 1101 92-93 0.17616274362803352 1101 94-95 -0.07940965376818454 1101 96-97 -0.2458031321206633 1101 98-99 -0.09546183446401102 1101 100-101 0.0240794476345485 1102 1 0.15601930498462124 1102 2 0.16084131259198742 1102 3 0.36544694739532346 1102 4 0.1946184340628747 1102 5 0.09534457038952837 1102 6 0.17083516199414106 1102 7 0.10145309224651555 1102 8 0.12383412255353932 1102 9 0.09870505061004309 1102 10-11 0.2299831520811324 1102 12-13 0.115980004958395 1102 14-15 -0.023431783965996544 1102 16-17 0.29440105274662187 1102 18-19 0.43903741487782355 1102 20-21 0.3782670035752389 1102 22-23 0.21968890571596944 1102 24-25 0.03789261827328261 1102 26-27 0.13881184541848768 1102 28-29 0.2141628215525273 1102 30-31 0.31982465009115657 1102 32-33 0.16993192078241037 1102 34-35 0.2191020973569593 1102 36-37 0.18013723042030705 1102 38-39 0.2567515124195481 1102 40-41 0.2955789034173151 1102 42-43 0.3689373895903145 1102 44-45 0.5932033399961227 1102 46-47 1.0065064662589265 1102 48-49 1.0236820144656278 1102 50-51 0.9896915296416608 1102 52-53 0.6997115681090236 1102 54-55 0.7454990112606055 1102 56-57 0.626215319188745 1102 58-59 0.45564595632983185 1102 60-61 0.7086321226672183 1102 62-63 0.7578127321215078 1102 64-65 0.8641958585625744 1102 66-67 0.48861532477761216 1102 68-69 0.4835850608552832 1102 70-71 0.5138954990991849 1102 72-73 0.8870778723010062 1102 74-75 0.9361422600444484 1102 76-77 0.8952500610657133 1102 78-79 0.8758205843815041 1102 80-81 0.6762598122746084 1102 82-83 0.8568239058534388 1102 84-85 1.0193949392276949 1102 86-87 1.2352320071103584 1102 88-89 0.9870414652220205 1102 90-91 1.0319068437125516 1102 92-93 0.5607980506952757 1102 94-95 0.2526373532569721 1102 96-97 0.5113757988903735 1102 98-99 -0.10194310611411339 1102 100-101 -0.48954676234311023 1103 1 0.48023475857112174 1103 2 0.46393994128397154 1103 3 0.5952135613193725 1103 4 0.644750290602957 1103 5 0.38264019275239747 1103 6 0.3897829468042673 1103 7 0.4805472378161326 1103 8 0.3599891858446753 1103 9 0.716413383943376 1103 10-11 0.9067203619967401 1103 12-13 0.7536744933550139 1103 14-15 0.8715345926162783 1103 16-17 0.9788452774301675 1103 18-19 0.924236518253359 1103 20-21 0.8307722778368429 1103 22-23 0.9197185734374926 1103 24-25 0.8168219431678025 1103 26-27 0.9245746355028786 1103 28-29 0.8754266664892398 1103 30-31 0.9756955625384123 1103 32-33 1.0092251697275643 1103 34-35 0.8725039960911332 1103 36-37 0.9030703791544852 1103 38-39 1.1620356632634241 1103 40-41 0.9354457283118265 1103 42-43 1.133721804147278 1103 44-45 1.3225183452703888 1103 46-47 1.0082206012800299 1103 48-49 0.8107864448453768 1103 50-51 0.8119785813294271 1103 52-53 0.8858435565056411 1103 54-55 0.908196796070726 1103 56-57 0.6033909521001348 1103 58-59 0.9038955607602119 1103 60-61 0.7750054085321878 1103 62-63 1.0098532120793209 1103 64-65 1.164752952444438 1103 66-67 0.9563236581109464 1103 68-69 0.7813039427118227 1103 70-71 0.9666381151329446 1103 72-73 1.162443774199744 1103 74-75 0.9325063159516205 1103 76-77 1.1281251929222513 1103 78-79 1.1365141497823465 1103 80-81 1.3579844958189113 1103 82-83 1.45208366323741 1103 84-85 1.4087574128563816 1103 86-87 1.5179884680808193 1103 88-89 1.1682881688085232 1103 90-91 1.3158763848517943 1103 92-93 1.153690983184724 1103 94-95 0.8651802066324983 1103 96-97 0.8368142218861578 1103 98-99 1.3895125900884189 1103 100-101 1.2305745456737647 1104 1 0.3840889252377835 1104 2 0.4778982746173064 1104 3 0.3742760613193745 1104 4 0.23933362393628954 1104 5 0.283161026085736 1104 6 0.20415794680426558 1104 7 0.3164847378161326 1104 8 0.3987391858446756 1104 9 0.40120505061003797 1104 10-11 0.4597932786634118 1104 12-13 0.4461224100216796 1104 14-15 0.577628342616272 1104 16-17 0.5974390274301697 1104 18-19 0.5641323515866858 1104 20-21 0.6191056111701769 1104 22-23 0.6070623234374892 1104 24-25 0.31640527650112915 1104 26-27 0.5415017188362086 1104 28-29 0.4702704164892353 1104 30-31 0.3378309792050871 1104 32-33 0.6288085030608883 1104 34-35 0.6700039960911326 1104 36-37 0.8447891291544849 1104 38-39 0.7974002465967587 1104 40-41 0.8440915616451576 1104 42-43 0.9879405541472792 1104 44-45 1.181320428603719 1104 46-47 0.8928039346133545 1104 48-49 1.0045364448453782 1104 50-51 0.7447389979960874 1104 52-53 0.8936039731723113 1104 54-55 0.736321796070726 1104 56-57 0.9202659521001308 1104 58-59 0.7806143107602068 1104 60-61 0.7068283251988561 1104 62-63 0.4877177954126495 1104 64-65 0.42668003577776403 1104 66-67 0.5386153247776164 1104 68-69 0.476512276045149 1104 70-71 0.37872144846627265 1104 72-73 0.5813500241997502 1104 74-75 0.35193339928495604 1104 76-77 0.2894272762555872 1104 78-79 0.5117224831156832 1104 80-81 0.19923449581891362 1104 82-83 0.3746878299040759 1104 84-85 0.5625594961897171 1104 86-87 0.9899155514141533 1104 88-89 0.7663610854751894 1104 90-91 1.0569701348517953 1104 92-93 1.10389931651806 1104 94-95 1.2127322899658282 1104 96-97 1.0906163052194877 1104 98-99 1.1595125900884184 1104 100-101 0.8272412123404322 1105 1 0.03615682647235374 1105 2 0.061200743753108355 1105 3 0.1359735921835714 1105 4 0.1668953523313519 1105 5 0.19720423596227477 1105 6 0.3107320208783406 1105 7 0.2101575773223061 1105 8 0.10389350683232834 1105 9 0.064137149375469 1105 10-11 0.19504019224365976 1105 12-13 0.24342179273773468 1105 14-15 0.1774277253323291 1105 16-17 -0.02675850343403141 1105 18-19 0.30589161084594707 1105 20-21 0.22930622845412785 1105 22-23 0.18734010121526978 1105 24-25 0.21026330119249081 1105 26-27 0.0836313484658362 1105 28-29 0.036103749822572695 1105 30-31 0.4097136952544673 1105 32-33 0.45660171293743446 1105 34-35 0.2961922676960711 1105 36-37 0.23500517853720027 1105 38-39 0.42346506141156937 1105 40-41 0.303752055472323 1105 42-43 0.11141277636949809 1105 44-45 0.10429882366544518 1105 46-47 0.03499529263805101 1105 48-49 -0.25364256750030734 1105 50-51 -0.3694739649668719 1105 52-53 -0.4432170144820091 1105 54-55 -0.03435721627495525 1105 56-57 0.0897721249396426 1105 58-59 0.11237357001947146 1105 60-61 0.021118448655649047 1105 62-63 0.1625634744249993 1105 64-65 0.1271275666419669 1105 66-67 -0.03484146534583488 1105 68-69 -0.01433648938694887 1105 70-71 -0.18891744042261394 1105 72-73 -0.5501623214792701 1105 74-75 -0.3215542550360304 1105 76-77 -0.32119000769503003 1105 78-79 -0.06684233169913156 1105 80-81 -0.6561358745514596 1105 82-83 -0.0313461207132093 1105 84-85 -0.08176149146460432 1105 86-87 -0.08144247327720322 1105 88-89 -0.09208027254950224 1105 90-91 -0.23463480341981224 1105 92-93 -0.12937228842021398 1105 94-95 -0.08421215447861385 1105 96-97 -0.19629727502742256 1105 98-99 0.19229036786619602 1105 100-101 0.46707145925401505 1106 1 -0.19496279890014634 1106 2 -0.28100566626939383 1106 3 -0.11541605690722889 1106 4 -0.019299380989814097 1106 5 -0.19608774238717075 1106 6 -0.22097752117603164 1106 7 -0.2940448188341165 1106 8 0.043332781903785644 1106 9 -0.04464470308454338 1106 10-11 0.10592628358951828 1106 12-13 0.0423970405635572 1106 14-15 -0.028769440634953014 1106 16-17 -0.07586146518066528 1106 18-19 0.16609047966550605 1106 20-21 -0.10513699966725909 1106 22-23 -0.1850189573506782 1106 24-25 0.17801857699374324 1106 26-27 -0.01447118756773591 1106 28-29 -0.21791923868317298 1106 30-31 -0.31035867596732913 1106 32-33 -0.1780695757568438 1106 34-35 -0.026972604894091035 1106 36-37 -0.4186468314366465 1106 38-39 -0.34085714256580246 1106 40-41 -0.2948370097834072 1106 42-43 -0.40612964289705644 1106 44-45 -0.47713400489381996 1106 46-47 -0.786986705780734 1106 48-49 -0.6148601068787585 1106 50-51 -0.5392696226935598 1106 52-53 -0.4184526770739936 1106 54-55 -0.3209442137814875 1106 56-57 -0.45318232376192924 1106 58-59 0.027991158050852505 1106 60-61 -0.010548522091781365 1106 62-63 -0.06308269719818327 1106 64-65 -0.20455764895130102 1106 66-67 -0.24562112842435369 1106 68-69 -0.15268107370854267 1106 70-71 0.14479903467317 1106 72-73 -0.11844061619434143 1106 74-75 -0.1441626598283463 1106 76-77 -0.19939045773456243 1106 78-79 -0.051781211465598886 1106 80-81 0.19034287020314622 1106 82-83 0.23101172152968985 1106 84-85 0.25457920062321193 1106 86-87 0.1796199849116924 1106 88-89 -0.2053199490075741 1106 90-91 0.025640085590712403 1106 92-93 0.24264315888259347 1106 94-95 0.2521042111480938 1106 96-97 0.19125669930815903 1106 98-99 0.5157441171820132 1106 100-101 0.7222104241630944 1107 1 0.2372183789194935 1107 2 0.23228663566243668 1107 3 0.3520610969488267 1107 4 0.3628074956702605 1107 5 0.4256491496011705 1107 6 0.3986531962104465 1107 7 0.19282084232919772 1107 8 0.01742683430073555 1107 9 0.15523711711835375 1107 10-11 0.3425842525351399 1107 12-13 0.5976811986202577 1107 14-15 0.5706420005735211 1107 16-17 0.7440898587840863 1107 18-19 0.5703853207078282 1107 20-21 0.473859174115546 1107 22-23 0.5131252688056662 1107 24-25 0.5702116898027967 1107 26-27 0.7730040941331211 1107 28-29 0.728186093448862 1107 30-31 0.7420411929818087 1107 32-33 0.644666579070396 1107 34-35 0.7408977015543172 1107 36-37 0.4829304593207553 1107 38-39 0.31648872640673886 1107 40-41 0.5865678086760369 1107 42-43 0.7041549246935972 1107 44-45 0.8406701910740253 1107 46-47 0.8352445521905594 1107 48-49 0.619352359334691 1107 50-51 0.8823251025091494 1107 52-53 0.9875885337423824 1107 54-55 0.8821056440517197 1107 56-57 0.5350640518626051 1107 58-59 0.815109560166384 1107 60-61 0.5182475651038487 1107 62-63 0.5834719521822507 1107 64-65 0.44511530893691287 1107 66-67 0.5513231632574289 1107 68-69 0.6550544375653473 1107 70-71 0.4508028023855175 1107 72-73 0.6290875538909546 1107 74-75 0.8680794800450542 1107 76-77 0.6733702691296948 1107 78-79 0.9339582313342092 1107 80-81 0.5748045670778197 1107 82-83 0.45149602467841987 1107 84-85 0.8324169783749902 1107 86-87 0.3719108008203307 1107 88-89 0.6630148859502469 1107 90-91 0.7136922251130748 1107 92-93 0.3637686751878917 1107 94-95 0.4839852590869711 1107 96-97 0.8715189180460925 1107 98-99 0.7959852741739262 1107 100-101 0.5297382432192919 1108 1 0.24658892523778775 1108 2 0.2203982746173061 1108 3 0.4992760613193745 1108 4 0.29183362393629153 1108 5 0.4506610260857329 1108 6 0.44165794680426984 1108 7 0.5139847378161306 1108 8 0.508739185844675 1108 9 0.6262050506100394 1108 10-11 0.4972932786634132 1108 12-13 0.5511224100216836 1108 14-15 0.5213783426162806 1108 16-17 0.7536890274301697 1108 18-19 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-2.624876362890383 2316 58-59 -2.368897074439026 2316 60-61 -2.2977210106645174 2316 62-63 -2.4383970053463635 2316 64-65 -2.496273000275366 2316 66-67 -2.7944966296815856 2316 68-69 -3.050359166080085 2316 70-71 -2.9884535989720575 2316 72-73 -3.0434459909805227 2316 74-75 -2.829029598817513 2316 76-77 -2.759567031144794 2316 78-79 -2.7508889969602173 2316 80-81 -2.376657344788299 2316 82-83 -2.287306951879607 2316 84-85 -2.457231775157531 2316 86-87 -2.1830635757205705 2316 88-89 -2.8207095976367675 2316 90-91 -2.5981342294745815 2316 92-93 -2.5835105506546157 2316 94-95 -2.5340751104136743 2316 96-97 -2.957543087569885 2316 98-99 -3.050952305547252 2316 100-101 -2.9797156187791103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1623.0 3 395.0 4 374.0 5 385.0 6 512.0 7 573.0 8 687.0 9 881.0 10 1098.0 11 1535.0 12 1889.0 13 2871.0 14 2510.0 15 2713.0 16 3377.0 17 4444.0 18 3939.0 19 2819.0 20 2713.0 21 2911.0 22 3090.0 23 3523.0 24 3859.0 25 4536.0 26 5065.0 27 6092.0 28 7125.0 29 8269.0 30 9982.0 31 12180.0 32 14877.0 33 19196.0 34 25638.0 35 36327.0 36 55257.0 37 84060.0 38 76286.0 39 11802.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56091366368668 19.02624920115741 14.57466200999408 21.838175125161833 2 14.63979277585773 21.538323997110908 40.46940691264731 23.352476314384056 3 20.24422954754006 25.894684830945152 30.760217406649254 23.10086821486553 4 12.271611735833163 16.850083178623446 39.071834800430125 31.806470285113264 5 12.803298247100267 38.61062282181794 34.61582810334319 13.970250827738598 6 29.441469156970523 36.87263182405115 18.196764110271445 15.489134908706891 7 26.239768557719444 34.57192586320444 21.605983629692354 17.58232194938376 8 24.41839405963416 34.12182265928747 20.752001016090453 20.707782264987923 9 25.68588525299663 18.068230999379033 21.998193553243137 34.2476901943812 10-11 24.093598474914533 26.429444216423835 27.914426805188207 21.562530503473425 12-13 26.960234923599263 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21.127211469398553 38-39 22.260723733938022 26.501913265306122 30.370134164777024 20.867228835978835 40-41 21.695416379838637 27.043937527969426 30.26152812528412 20.999117966907818 42-43 21.029941025074677 27.746562552044512 29.19784416115167 22.025652261729135 44-45 21.6024050842252 27.5716445767477 28.714413967443946 22.111536371583153 46-47 21.94568799536966 27.35243742543616 28.219681309724898 22.482193269469285 48-49 22.13290612831273 27.121729802275908 28.571698612528827 22.17366545688254 50-51 22.043599530761025 27.72632924462212 28.90226196340834 21.327809261208515 52-53 21.68730920810139 28.072399323308982 28.6444206069427 21.59587086164693 54-55 20.999033734250933 27.360440749482017 29.118264785165337 22.52226073110171 56-57 22.05944354492987 28.06072136738813 27.9051860459898 21.974649041692203 58-59 22.33564455108374 27.402690333032627 28.158047619272537 22.103617496611104 60-61 21.747607879980315 27.036296961621037 29.251198863160234 21.96489629523842 62-63 22.469671253037006 26.950920049749655 28.74589996444766 21.833508732765676 64-65 21.988235419209182 27.636132078035157 28.80904589698726 21.5665866057684 66-67 21.440783402787705 27.45052631330204 28.566719323596722 22.54197096031353 68-69 21.156564033238485 28.061513882455262 28.671618721698604 22.110303362607652 70-71 21.86494112976031 27.70588040667968 27.83444488034523 22.594733583214772 72-73 21.663246172768616 27.072116299155567 28.367650516500486 22.89698701157533 74-75 21.51894396801278 28.153999938558826 28.031590215729413 22.29546587769898 76-77 22.040507274110404 26.98543760424492 28.214405526339416 22.759649595305255 78-79 21.663700384652177 27.096425226663705 28.76789660095968 22.471977787724434 80-81 21.81135088338847 27.927335260067814 28.15079551614949 22.11051834039422 82-83 21.680476727635675 27.51873660089416 28.331630453399164 22.469156218071003 84-85 21.72665592561269 27.217468544618683 28.064035265935583 22.991840263833044 86-87 21.85039416582605 27.40840398866995 28.205358387333774 22.53584345817023 88-89 22.331096006596216 26.778344000207905 28.083057506621024 22.807502486574858 90-91 21.822422122272197 26.82724831437709 28.647496582677928 22.702832980672785 92-93 21.91040840101421 27.58390498762958 28.302263538341204 22.203423073015003 94-95 21.612954302148886 27.687055859756253 27.91239019825168 22.787599639843176 96-97 21.105277087252 27.18519901320453 28.17849277389718 23.531031125646283 98-99 21.91412286795877 27.2367233320573 27.53836374549253 23.310790054491406 100-101 22.415385778479187 26.4436275320478 28.328458634359777 22.812528055113237 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 463.0 1 269.0 2 90.5 3 114.5 4 106.5 5 105.0 6 173.5 7 314.5 8 466.5 9 586.0 10 588.5 11 563.0 12 615.0 13 632.5 14 666.0 15 734.0 16 819.5 17 842.0 18 857.0 19 924.0 20 991.5 21 1114.0 22 1286.5 23 1553.5 24 1868.0 25 2233.0 26 2722.0 27 3393.0 28 3989.0 29 4553.0 30 5191.0 31 5912.0 32 6846.5 33 7675.5 34 8546.0 35 9399.5 36 10499.5 37 11524.5 38 11797.5 39 11893.0 40 12215.5 41 12598.0 42 12790.5 43 13168.5 44 13806.0 45 14329.0 46 15447.0 47 18693.5 48 29286.0 49 29017.0 50 17883.5 51 14207.5 52 12503.5 53 11707.5 54 10939.5 55 9912.5 56 9032.5 57 8083.5 58 7308.0 59 6452.5 60 5472.0 61 4441.0 62 3477.5 63 2661.5 64 1963.5 65 1503.5 66 1067.5 67 772.5 68 562.5 69 394.0 70 284.0 71 199.0 72 142.0 73 111.0 74 88.5 75 63.5 76 47.0 77 28.0 78 18.5 79 14.0 80 9.5 81 7.5 82 5.5 83 2.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32062809256843317 2 0.08650376690995851 3 0.09520115651775325 4 0.10013751278163677 5 0.10859983780543706 6 0.057825887662635314 7 0.059941468918585386 8 0.06017653350257983 9 0.06370250226249662 10-11 0.06076419496256597 12-13 0.060059001210582615 14-15 0.05806095224662976 16-17 0.059706404334590936 18-19 0.06711093873041618 20-21 0.06934405227836347 22-23 0.08497584711399457 24-25 0.094025833597781 26-27 0.09049986483786421 28-29 0.08685636378595019 30-31 0.0810972814780861 32-33 0.07228235957829414 34-35 0.3242715936203472 36-37 0.5093849535159786 38-39 0.4830577201085999 40-41 0.4610791815051185 42-43 0.493518094096353 44-45 0.5042135326681005 46-47 0.4983369180682392 48-49 0.516907020203801 50-51 0.5116180670639259 52-53 0.512205728523912 54-55 0.5020979514121505 56-57 0.4791791544726914 58-59 0.4637824242210547 60-61 0.4194727501381004 62-63 0.49257783576037517 64-65 0.5160842941598205 66-67 0.5254868775195984 68-69 0.5385329619312906 70-71 0.5361823160913461 72-73 0.5243115545996263 74-75 0.5279550556515402 76-77 0.5013927576601671 78-79 0.48082460656065257 80-81 0.4874064149124972 82-83 0.49657393368828084 84-85 0.49763172431625585 86-87 0.4973966597322615 88-89 0.5035083389161172 90-91 0.517964810831776 92-93 0.5237238931396401 94-95 0.5326563473314293 96-97 0.5234888285556457 98-99 0.5239589577236345 100-101 0.5032732743321228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 425415.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.21377480196516 #Duplication Level Percentage of deduplicated Percentage of total 1 86.0129706932804 61.25308324999299 2 9.536389561001197 13.582445972419013 3 2.341404911852676 5.002208463386746 4 0.8252480828944574 2.350761245239975 5 0.4244806103015746 1.5114433294908534 6 0.21485596989950737 0.9180422793168793 7 0.1284517231908784 0.6403272461767705 8 0.08992964359973478 0.5123383509866 9 0.06604289132468692 0.42328472310602216 >10 0.3212827327919195 3.9383203024991964 >50 0.014851212699020858 0.6937927607852609 >100 0.01617132042509589 2.5608285552756826 >500 0.004620377264313111 2.3333246968407653 >1k 0.0033002694745093647 4.279798824483313 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2982 0.7009625894714573 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2728 0.6412561851368663 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2460 0.5782588766263531 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1876 0.4409811595735928 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1869 0.4393357074856317 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1539 0.36176439476746236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1533 0.36035400726349565 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1134 0.2665632382497091 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1066 0.2505788465380863 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1023 0.2404710694263249 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 994 0.23365419649048574 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 939 0.2207256443707909 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.21296851309897397 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 811 0.19063737761950095 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 783 0.1840555692676563 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 758 0.178178954667795 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 645 0.15161665667642185 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 629 0.1478556233325106 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 628 0.14762055874851615 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 593 0.1393932983087103 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 587 0.1379829108047436 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 586 0.13774784622074915 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 553 0.12999071494893222 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 516 0.12129332534113749 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 456 0.10718945030147034 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 455 0.10695438571747587 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 449 0.10554399821350915 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 444 0.10436867529353691 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1753229199722624E-4 0.0 16-17 0.0 0.0 0.0 2.3506458399445249E-4 0.0 18-19 0.0 0.0 0.0 2.3506458399445249E-4 0.0 20-21 0.0 0.0 0.0 2.3506458399445249E-4 0.0 22-23 0.0 0.0 0.0 5.876614599861312E-4 0.0 24-25 0.0 0.0 0.0 7.051937519833574E-4 0.0 26-27 0.0 0.0 0.0 0.0017629843799583937 0.0 28-29 0.0 0.0 0.0 0.006464276059847443 0.0 30-31 0.0 0.0 0.0 0.01798244067557561 0.0 32-33 0.0 0.0 0.0 0.03831552719109575 0.0 34-35 0.0 0.0 0.0 0.07674858667418874 0.0 36-37 0.0 0.0 0.0 0.12822773056897382 0.0 38-39 0.0 0.0 0.0 0.20051009014726795 0.0 40-41 0.0 0.0 0.0 0.2691489486736481 0.0 42-43 0.0 0.0 0.0 0.34660272909982015 0.0 44-45 0.0 0.0 0.0 0.4483856939694181 0.0 46-47 0.0 0.0 0.0 0.5672108411786139 0.0 48-49 0.0 0.0 0.0 0.6979067498795295 0.0 50-51 0.0 0.0 0.0 0.8324812242163535 0.0 52-53 0.0 0.0 0.0 0.9624719391652856 0.0 54-55 0.0 0.0 0.0 1.098221736422082 0.0 56-57 0.0 0.0 0.0 1.2640598004301682 0.0 58-59 0.0 0.0 0.0 1.4757354583171725 0.0 60-61 0.0 0.0 0.0 1.697753957899933 0.0 62-63 0.0 0.0 0.0 1.9229458293666184 0.0 64-65 0.0 0.0 0.0 2.1517812018852176 0.0 66-67 0.0 0.0 0.0 2.426219103698741 0.0 68-69 0.0 0.0 0.0 2.74238096917128 0.0 70-71 0.0 0.0 0.0 3.0804038409553023 0.0 72-73 0.0 0.0 0.0 3.4203072294112804 0.0 74-75 0.0 0.0 0.0 3.777840461666843 0.0 76-77 0.0 0.0 0.0 4.163934040877731 0.0 78-79 0.0 0.0 0.0 4.616550897359049 0.0 80-81 0.0 0.0 0.0 5.125818318583031 0.0 82-83 0.0 0.0 0.0 5.616868234547442 0.0 84-85 0.0 0.0 0.0 6.190543351785903 0.0 86-87 0.0 0.0 0.0 6.775971698224087 0.0 88-89 0.0 0.0 0.0 7.376561710329913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATCC 25 0.0050919964 56.792725 3 GTATAGA 135 4.297508E-7 28.085459 1 GTCTAAC 70 0.008477398 27.082407 1 GTATAGG 110 9.607333E-5 25.851389 1 AGTACTT 1625 0.0 22.11898 12-13 GTATAGT 130 2.9818912E-4 21.874252 1 GAGTACT 1465 0.0 21.62928 12-13 GTACATG 2195 0.0 21.591898 1 TACATGG 2155 0.0 21.083147 2 GTATTAG 115 0.0037678182 20.606178 1 GTGTAGC 305 2.1282176E-10 20.200813 1 ACATGGG 2220 0.0 19.613104 3 GCCCTAT 125 0.0061030923 18.957685 1 CATGGGG 675 0.0 18.930908 4 TAGCCCT 350 6.730261E-11 18.930908 4 GTCCTAT 155 9.7558793E-4 18.346146 1 CCAATAC 155 9.848948E-4 18.320234 3 TCTAGAC 155 9.848948E-4 18.320234 3 GTACTTT 1725 0.0 18.233202 14-15 CTTTGCG 130 0.007715878 18.202795 2 >>END_MODULE