FastQCFastQC Report
Thu 26 May 2016
SRR938904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427414
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30410.7114881590214639No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26840.6279625842859616No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24850.5814035104137909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.4255826903189881No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17440.4080353006686725No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15670.3666234610939277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.33503815972335954No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11490.2688260094428353No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10930.255723958503933No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10060.23536898650956684No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10030.23466709092355423No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8980.21010074541311236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.20752712826439937No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7840.1834287131446326No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7610.17804751365186913No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7360.1721983837684306No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6980.16330770634560401No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6300.14739807306265118No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG6120.14318669954657545No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT6070.14201687356988774No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5760.13476395251442397No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5670.13265826575638606No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5380.12587327509159738No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5220.1221298319661967No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA4880.11417501532472028No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4500.10528433790189373No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG4290.10037106879980534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGT905.8976E-736.7731364
CTTTGCG1703.6379788E-1233.373942
TTTGCGT1703.6379788E-1233.373943
TCGCGCT300.003908657531.73541860-61
TTGCGTT1859.094947E-1230.6679444
CGCTCCG458.8513165E-426.43682748-49
CGCGGAA458.857411E-426.4337182-83
TACATGG24050.023.7873152
GTACATG24900.023.7849561
CCTATAC1000.001697242723.6398743
GAGTACT15100.022.54398712-13
CAGTACC2103.6066922E-822.5141664
ACATGGG24250.022.0313873
CTAGTAC1100.002953002221.4907953
TAGACAG2257.483868E-821.0132225
TAGTACC1150.00382100520.5564144
TAGTACT1404.9991027E-420.262754
TAGCCCT3750.020.1726934
GTATAGC1656.435151E-520.100451
ATAGCCC1908.56861E-619.9072633
Produced by FastQC (version 0.11.5)