##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938904_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427414 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77212023939319 33.0 31.0 34.0 30.0 34.0 2 31.832518354569576 33.0 31.0 34.0 30.0 34.0 3 31.876396187303175 34.0 31.0 34.0 30.0 34.0 4 35.39921013350054 37.0 35.0 37.0 33.0 37.0 5 35.44625117567511 37.0 35.0 37.0 33.0 37.0 6 35.52694109224312 37.0 35.0 37.0 33.0 37.0 7 35.45281857870823 37.0 35.0 37.0 33.0 37.0 8 35.43998792739592 37.0 35.0 37.0 33.0 37.0 9 37.04369066057733 39.0 37.0 39.0 33.0 39.0 10-11 36.92356006120529 39.0 37.0 39.0 33.0 39.0 12-13 36.81778439639319 39.0 37.0 39.0 33.0 39.0 14-15 37.96920199151174 40.0 38.0 41.0 33.0 41.0 16-17 37.91199048229585 40.0 38.0 41.0 33.0 41.0 18-19 37.909359543674285 40.0 38.0 41.0 33.0 41.0 20-21 37.79812079155105 40.0 38.0 41.0 32.0 41.0 22-23 37.877819865516805 40.0 38.0 41.0 32.5 41.0 24-25 37.81493002101007 40.0 38.0 41.0 32.0 41.0 26-27 37.700253150341354 40.0 37.5 41.0 32.0 41.0 28-29 37.562018324154096 40.0 37.0 41.0 32.0 41.0 30-31 37.41468693117211 40.0 37.0 41.0 32.0 41.0 32-33 37.21090324603312 40.0 36.5 41.0 31.0 41.0 34-35 36.895308763868286 40.0 36.0 41.0 30.5 41.0 36-37 36.61099659814606 39.5 36.0 41.0 30.0 41.0 38-39 36.38622389533333 39.0 35.5 41.0 30.0 41.0 40-41 36.241266079258054 39.0 35.0 41.0 30.0 41.0 42-43 36.046784850285675 39.0 35.0 41.0 29.5 41.0 44-45 35.96796314580243 39.0 35.0 41.0 28.5 41.0 46-47 35.7000952238345 39.0 35.0 41.0 27.0 41.0 48-49 35.492400810455436 39.0 35.0 41.0 26.0 41.0 50-51 34.32543037897682 38.0 34.0 40.0 24.0 40.5 52-53 34.453554399247565 38.0 34.5 39.5 24.0 40.5 54-55 34.960827207344636 39.0 35.0 40.5 24.0 41.0 56-57 34.88680061953984 39.0 35.0 41.0 24.0 41.0 58-59 34.71049146728932 39.0 34.5 40.5 23.0 41.0 60-61 34.41674231541316 38.0 34.0 40.0 23.0 41.0 62-63 34.069470115625606 38.0 34.0 40.0 22.0 41.0 64-65 33.64766596321131 37.0 33.5 40.0 21.5 41.0 66-67 33.24291085458127 37.0 33.0 39.5 20.0 41.0 68-69 32.8575771968162 36.0 33.0 39.0 18.5 41.0 70-71 32.4080037153673 36.0 32.0 39.0 17.5 40.0 72-73 31.94310317397184 35.0 31.5 38.0 13.5 40.0 74-75 31.48522275826248 35.0 31.0 37.0 10.0 39.5 76-77 31.043206352622985 35.0 31.0 37.0 7.5 39.0 78-79 30.587176601608746 35.0 31.0 36.0 4.5 39.0 80-81 30.160104722821433 34.0 30.0 36.0 2.0 37.5 82-83 29.77897541961658 34.0 30.0 35.0 2.0 37.0 84-85 29.413092458365895 34.0 30.0 35.0 2.0 37.0 86-87 29.00317841717866 34.0 29.0 35.0 2.0 36.0 88-89 28.541825957970495 34.0 29.0 35.0 2.0 36.0 90-91 28.093720608122336 34.0 28.5 35.0 2.0 35.5 92-93 27.699026002891813 34.0 27.0 35.0 2.0 35.0 94-95 27.22461009700197 34.0 26.5 35.0 2.0 35.0 96-97 26.611656379996912 33.0 25.0 35.0 2.0 35.0 98-99 25.914554740836753 33.0 24.0 35.0 2.0 35.0 100-101 23.33878862180462 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23302866501158803 1101 2 0.283941058785647 1101 3 0.3397842138366336 1101 4 0.44275419436989694 1101 5 0.1913001946218813 1101 6 0.24236593365286296 1101 7 0.16059550500282427 1101 8 0.46734069847855864 1101 9 0.26446871474860245 1101 10-11 0.6125055335143301 1101 12-13 0.5399387543453855 1101 14-15 0.3664465438058002 1101 16-17 0.1652641493648801 1101 18-19 0.303665830016719 1101 20-21 0.659163255279033 1101 22-23 0.4277587046349325 1101 24-25 0.5194273018553801 1101 26-27 0.5617617753652695 1101 28-29 0.7482440641430017 1101 30-31 0.700320632806644 1101 32-33 0.7399934024685493 1101 34-35 0.6682232908925414 1101 36-37 0.48572220147120504 1101 38-39 0.7100601797851596 1101 40-41 0.5169159265876075 1101 42-43 0.5521190963495854 1101 44-45 0.3656372834082404 1101 46-47 0.5289758177282735 1101 48-49 0.5954050630211043 1101 50-51 0.5382471767122965 1101 52-53 0.7576251978552335 1101 54-55 0.5452893968638222 1101 56-57 0.5387940381888967 1101 58-59 0.7354757728857706 1101 60-61 0.31586878649976313 1101 62-63 0.45795065534900914 1101 64-65 0.7761595928694263 1101 66-67 0.783814191592036 1101 68-69 0.8613055248689179 1101 70-71 0.4472453748823497 1101 72-73 0.37982754787988426 1101 74-75 0.13305395114122476 1101 76-77 0.09522282871798637 1101 78-79 -0.25535240976699924 1101 80-81 -0.6051329303737951 1101 82-83 -0.41132185107079877 1101 84-85 -0.34496241653880233 1101 86-87 -0.19320654526062242 1101 88-89 0.21201074199285586 1101 90-91 0.09333514376778851 1101 92-93 0.10307478381634283 1101 94-95 -0.10117890318657885 1101 96-97 -0.5018538497992147 1101 98-99 -0.44570589467318555 1101 100-101 0.1689324341619738 1102 1 0.22776233462867168 1102 2 0.004425814979928333 1102 3 0.16456769151818307 1102 4 0.12825909586413786 1102 5 0.25740850336843124 1102 6 0.14422183923961995 1102 7 0.3321700108877934 1102 8 0.009480859006465892 1102 9 0.40169786357002124 1102 10-11 0.15211087598442674 1102 12-13 0.2804038411129355 1102 14-15 0.6685562484471461 1102 16-17 0.24820885289585704 1102 18-19 0.4879969108834459 1102 20-21 0.44756146599569746 1102 22-23 0.5746956672947476 1102 24-25 0.09107477496853988 1102 26-27 0.5540970439163928 1102 28-29 0.5608864295502016 1102 30-31 0.32070704186726573 1102 32-33 0.3855979200917261 1102 34-35 0.2864333730599853 1102 36-37 0.5848434222446599 1102 38-39 0.5359096134960168 1102 40-41 0.5341108125366603 1102 42-43 0.4945479533020958 1102 44-45 0.7669602109803364 1102 46-47 0.5453109593798686 1102 48-49 0.5002780048693936 1102 50-51 0.25249799362800474 1102 52-53 0.09011782182020056 1102 54-55 0.09531319052519649 1102 56-57 0.35540835880055255 1102 58-59 0.33847377421822245 1102 60-61 0.2608768763446321 1102 62-63 0.1817157643239824 1102 64-65 0.30560345569104186 1102 66-67 0.23448517284263204 1102 68-69 0.16612770690629475 1102 70-71 0.2421281514440068 1102 72-73 0.0720168819746192 1102 74-75 -0.023425855971492382 1102 76-77 0.07132130275117277 1102 78-79 0.1624515939130866 1102 80-81 -0.21852400299205144 1102 82-83 -0.05893455371348111 1102 84-85 -0.37057074114779454 1102 86-87 -0.12345622008058044 1102 88-89 -0.013369163466997236 1102 90-91 -0.34659379249692535 1102 92-93 -0.008435003309696043 1102 94-95 -0.28226452864262086 1102 96-97 -0.7116219409130764 1102 98-99 -0.7008627742138067 1102 100-101 -0.0693023934132917 1103 1 -0.06660200514845016 1103 2 0.04256980337985539 1103 3 0.06206080085011578 1103 4 0.20029704610133336 1103 5 0.19645187238948836 1103 6 -0.10860474092650918 1103 7 -0.10826399182472812 1103 8 -0.11502616916246211 1103 9 -0.0444299247286537 1103 10-11 0.16413595114114798 1103 12-13 0.251081782850072 1103 14-15 0.23755042966077866 1103 16-17 0.17871154353767338 1103 18-19 0.21157643325545905 1103 20-21 0.14072517326886924 1103 22-23 0.2941970391783144 1103 24-25 0.3408263359157999 1103 26-27 0.44683074850204463 1103 28-29 0.28469407834800364 1103 30-31 0.24221905131732768 1103 32-33 0.2952161052053768 1103 34-35 0.4225842240028044 1103 36-37 0.39924851227647196 1103 38-39 0.49128433458803045 1103 40-41 0.47400106185072133 1103 42-43 0.3658190237462833 1103 44-45 0.4964147700713326 1103 46-47 0.4666979680486776 1103 48-49 0.07250345627846855 1103 50-51 0.04754671268249666 1103 52-53 -0.15946498807235798 1103 54-55 0.2957613183259937 1103 56-57 0.3816710229422 1103 58-59 0.16476331352783546 1103 60-61 0.26247063630562906 1103 62-63 0.09179957734781397 1103 64-65 -0.09074277147288257 1103 66-67 -0.08843872043170364 1103 68-69 0.3019191806030008 1103 70-71 0.3038222870323537 1103 72-73 0.22278344926566263 1103 74-75 0.08778421409144599 1103 76-77 0.07077129931364823 1103 78-79 0.04154458824429952 1103 80-81 0.16328463331192822 1103 82-83 0.06973500047123338 1103 84-85 -0.019093544415312635 1103 86-87 -0.016783678377198186 1103 88-89 0.08759709257210346 1103 90-91 -0.11563609901133987 1103 92-93 -0.14876713038549028 1103 94-95 -0.4250685461677328 1103 96-97 -0.31680697331952956 1103 98-99 -0.22428792062097003 1103 100-101 0.09515800946422459 1104 1 -0.04914564604620608 1104 2 -0.02725563302912093 1104 3 0.14934259636133973 1104 4 0.015758392734745996 1104 5 -0.0030493744933011158 1104 6 0.0235648351333424 1104 7 0.061312068025650035 1104 8 -0.07512591978590422 1104 9 0.002951621406005245 1104 10-11 -0.13386903639002412 1104 12-13 0.016667817762794357 1104 14-15 0.07919631494755919 1104 16-17 0.12010805226585575 1104 18-19 0.16045423874174247 1104 20-21 0.03723390144842398 1104 22-23 0.27300003169702336 1104 24-25 0.07524030100307755 1104 26-27 0.12264122231750463 1104 28-29 -0.13051789172681083 1104 30-31 0.2185282782499911 1104 32-33 0.20544054410812862 1104 34-35 0.2530081641524333 1104 36-37 -0.04339487924472962 1104 38-39 0.1483915665082236 1104 40-41 0.22213073766119606 1104 42-43 0.5603327394570101 1104 44-45 0.4415519271785655 1104 46-47 0.842009688746927 1104 48-49 0.6136505884480457 1104 50-51 0.759516787495464 1104 52-53 0.6485150618029536 1104 54-55 0.7421453582262387 1104 56-57 0.8692021950120292 1104 58-59 0.5700002212585176 1104 60-61 0.5654631550088709 1104 62-63 0.6740938416869682 1104 64-65 0.5763395227914572 1104 66-67 0.7345039229598243 1104 68-69 0.2832159387077411 1104 70-71 0.17289959376552133 1104 72-73 0.43475352407863 1104 74-75 0.3633453113483043 1104 76-77 0.7029408753734998 1104 78-79 0.39316553587522307 1104 80-81 0.44009261336180217 1104 82-83 0.15078238201736838 1104 84-85 0.28514585708094486 1104 86-87 0.23259288022629576 1104 88-89 0.15617564618806057 1104 90-91 0.15369058428043303 1104 92-93 -0.2447771054478345 1104 94-95 0.01009354859535705 1104 96-97 -0.32054762169858364 1104 98-99 -0.47241759643144476 1104 100-101 0.12757696208267788 1105 1 0.059031154159512766 1105 2 0.20025711986084005 1105 3 0.09035337875786453 1105 4 0.037095936166338106 1105 5 -0.09509145155484333 1105 6 0.2678402377538873 1105 7 0.2973347212247859 1105 8 0.18371139938619052 1105 9 0.2240595231145619 1105 10-11 0.13627993171729003 1105 12-13 0.4331057397444127 1105 14-15 0.34481524125290264 1105 16-17 0.34817098845056904 1105 18-19 0.24314918004556318 1105 20-21 0.4250862131152999 1105 22-23 0.5799245668818429 1105 24-25 0.39887665376625847 1105 26-27 0.4638714800066168 1105 28-29 0.42113056506884305 1105 30-31 0.3812270469158605 1105 32-33 0.4846675365789892 1105 34-35 0.5870770686298457 1105 36-37 0.6536395131914503 1105 38-39 0.5429776432827111 1105 40-41 0.3999715074393819 1105 42-43 0.44039278119917924 1105 44-45 0.24562089145851473 1105 46-47 0.6503126722952501 1105 48-49 0.46229636011001674 1105 50-51 0.36720021681185955 1105 52-53 0.4228651577860205 1105 54-55 0.4248510639858196 1105 56-57 0.0479310323910056 1105 58-59 0.27151681347103107 1105 60-61 0.49409481327328564 1105 62-63 0.0545994122928235 1105 64-65 0.25665013714287 1105 66-67 0.2250551228976292 1105 68-69 0.2298481591159529 1105 70-71 0.07822919542085316 1105 72-73 0.44399899553189215 1105 74-75 0.24481669284659802 1105 76-77 0.31955880297655526 1105 78-79 0.35345067434798594 1105 80-81 0.4096471443006422 1105 82-83 0.09063000516573183 1105 84-85 0.4291443739781471 1105 86-87 0.43285361926598753 1105 88-89 0.8017080980320728 1105 90-91 0.6390808257032532 1105 92-93 0.12955385372497474 1105 94-95 0.2575923076928177 1105 96-97 -0.14354707749553697 1105 98-99 0.5196615581999602 1105 100-101 0.2317469926417708 1106 1 0.158416434667501 1106 2 0.056051357709993255 1106 3 0.2457389294722212 1106 4 -0.05254502711937192 1106 5 0.1600171376301418 1106 6 -0.08506616951466128 1106 7 0.1143543449624147 1106 8 0.09746490362505256 1106 9 0.21598710780737207 1106 10-11 0.03614313974241412 1106 12-13 0.33912458663872513 1106 14-15 0.1321712719213295 1106 16-17 0.26118142517162113 1106 18-19 0.16193527792781026 1106 20-21 0.20505378835088095 1106 22-23 0.06476767830819341 1106 24-25 -0.08960579970758431 1106 26-27 -0.15232570309196802 1106 28-29 -0.11262834329741622 1106 30-31 -0.08045431222218014 1106 32-33 0.08681398843159371 1106 34-35 0.16801232042840297 1106 36-37 0.01156162620526402 1106 38-39 0.3408886102837627 1106 40-41 0.13116683286918374 1106 42-43 0.683223868442056 1106 44-45 0.7502988392411325 1106 46-47 0.59443906132487 1106 48-49 0.8171617635619057 1106 50-51 0.6257286053686215 1106 52-53 0.6039287576103831 1106 54-55 0.8227215000313493 1106 56-57 1.0494762750700986 1106 58-59 0.9402775795060876 1106 60-61 0.8292520396048886 1106 62-63 1.0349756746632082 1106 64-65 0.7912365525176099 1106 66-67 0.8438783741557714 1106 68-69 0.9627407183080336 1106 70-71 0.7033516326823133 1106 72-73 0.49437559566024447 1106 74-75 0.6486063371874593 1106 76-77 0.5150362238567538 1106 78-79 0.45346080708686287 1106 80-81 0.8388024116360704 1106 82-83 0.832034065016444 1106 84-85 0.9398090816486331 1106 86-87 1.0836372177393194 1106 88-89 1.7033698672082807 1106 90-91 1.3993908875129577 1106 92-93 0.9946448794959082 1106 94-95 0.9998492063992046 1106 96-97 1.2187321132363813 1106 98-99 1.1751653242012452 1106 100-101 1.6197971159234292 1107 1 0.2322822338854138 1107 2 0.0785854154553185 1107 3 0.0382260388607385 1107 4 0.2770278235802408 1107 5 0.2579627144489578 1107 6 0.0274310908699249 1107 7 0.10500504688071999 1107 8 0.23378239909747833 1107 9 0.3191696757786744 1107 10-11 -0.02654214477385608 1107 12-13 0.1783581864357089 1107 14-15 0.256189336080233 1107 16-17 0.1278803904621313 1107 18-19 0.2597943405756098 1107 20-21 0.3734449708265757 1107 22-23 0.1166617618924164 1107 24-25 0.29771237634297876 1107 26-27 0.42374043987557997 1107 28-29 0.3040988832889653 1107 30-31 0.2939386466778231 1107 32-33 0.5208007921367042 1107 34-35 0.5453669477830374 1107 36-37 0.24529984585898035 1107 38-39 0.27158787549007 1107 40-41 0.5859141538136896 1107 42-43 0.47493811790278784 1107 44-45 0.5324273185527133 1107 46-47 0.41117032523924024 1107 48-49 0.47288150378868465 1107 50-51 0.8563263606142684 1107 52-53 0.6765285661256826 1107 54-55 0.7759490553715764 1107 56-57 0.4920798902089274 1107 58-59 0.5813937276644836 1107 60-61 0.7794429475422788 1107 62-63 0.4735448456267477 1107 64-65 0.30747237861258725 1107 66-67 0.19033302342732128 1107 68-69 0.4310159717945581 1107 70-71 0.614259339364672 1107 72-73 0.2337603068759364 1107 74-75 0.49839466584903036 1107 76-77 0.571610724093226 1107 78-79 0.7498352865599358 1107 80-81 0.8648201066678496 1107 82-83 0.585748406758956 1107 84-85 0.537953641060799 1107 86-87 0.7760591531190038 1107 88-89 0.8698000618074886 1107 90-91 0.687696478635452 1107 92-93 0.668241331261374 1107 94-95 0.7237363457793045 1107 96-97 0.7436193564334843 1107 98-99 1.0593978281520613 1107 100-101 1.2741730026936828 1108 1 0.25972166558016596 1108 2 0.275226931245772 1108 3 0.24668788562045663 1108 4 0.21962225553637182 1108 5 0.358299027551233 1108 6 0.32010480907555205 1108 7 0.33366310532601773 1108 8 0.5292732477798126 1108 9 0.6197429763519722 1108 10-11 0.602145537105315 1108 12-13 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-1.3972318372436519 2316 54-55 -1.43597312582434 2316 56-57 -1.4832105348021685 2316 58-59 -1.394104879119034 2316 60-61 -1.1346580503576007 2316 62-63 -1.4307441564276218 2316 64-65 -1.6180534519411722 2316 66-67 -1.4945301027765794 2316 68-69 -1.6528401036816263 2316 70-71 -1.5585887245448475 2316 72-73 -1.3924893556865747 2316 74-75 -1.6154716128217217 2316 76-77 -1.4961238464346422 2316 78-79 -1.4416004356020444 2316 80-81 -1.479672684752316 2316 82-83 -1.4133891258981315 2316 84-85 -1.7243462413508155 2316 86-87 -1.9129859102277358 2316 88-89 -1.9414225371246054 2316 90-91 -1.9846414255460267 2316 92-93 -1.6589929624533681 2316 94-95 -2.5639113832092058 2316 96-97 -2.8277572512559743 2316 98-99 -2.9171577014760963 2316 100-101 -3.246295154645985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1793.0 3 382.0 4 369.0 5 408.0 6 475.0 7 572.0 8 729.0 9 820.0 10 1022.0 11 1377.0 12 1917.0 13 2737.0 14 2651.0 15 2508.0 16 3244.0 17 4306.0 18 3857.0 19 2637.0 20 2642.0 21 2663.0 22 2835.0 23 3207.0 24 3794.0 25 4191.0 26 4919.0 27 5834.0 28 6732.0 29 8023.0 30 9619.0 31 11824.0 32 14780.0 33 18822.0 34 24962.0 35 35524.0 36 54801.0 37 85743.0 38 81149.0 39 13542.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.553944342765966 18.91970528884509 14.63067248580412 21.895677882584824 2 14.540502691905083 21.49144889829774 40.718840525407415 23.24920788438976 3 20.288897944271923 25.89800516156833 30.704305834633093 23.108791059526652 4 12.3220195670148 16.760316950974723 39.02277353182978 31.894889950180705 5 12.80277140802612 38.63083261698026 34.58638515357283 13.980010821420791 6 29.312117273759764 37.023428992939685 18.168065849403444 15.496387883897103 7 26.224758889133497 34.44079609913299 21.642832718943655 17.69161229278986 8 24.545194802761618 34.05348059466599 20.760381830274756 20.64094277229763 9 25.72985050506943 18.226999023352874 21.792814968698107 34.25033550287959 10-11 24.055948150186474 26.426074788789293 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21.765480926859286 36-37 22.138080020332186 26.491732989443268 30.192590988887897 21.177596001336653 38-39 22.253253040111712 26.621108615835993 30.290888429047197 20.834749915005098 40-41 21.632018025603696 27.07486129175653 30.187241428783107 21.105879253856667 42-43 21.04556126986218 27.650133865344166 29.26357592211813 22.040728942675518 44-45 21.626639558360512 27.425393329285914 28.84399075682061 22.103976355532964 46-47 21.944189548481212 27.40041410790334 28.269923060633868 22.38547328298158 48-49 22.152378352427306 27.16336875994314 28.549054401340502 22.135198486289053 50-51 22.046697478279693 27.661972759019925 28.863649199431464 21.42768056326892 52-53 21.697947811025884 28.13173262358023 28.549324428473433 21.620995136920456 54-55 21.1331436760107 27.263240128289297 29.107928193761072 22.49568800193893 56-57 22.085326006412622 27.98689246504932 27.85951037287785 22.06827115566021 58-59 22.31088770284924 27.416843278895207 28.162966238641733 22.109302779613813 60-61 21.80241921263915 26.990443052110642 29.26706555854659 21.940072176703616 62-63 22.3601629564622 26.958871492383324 28.83089443710495 21.850071114049523 64-65 21.869571971248217 27.666647837732246 28.947250740212482 21.516529450807056 66-67 21.521677979287723 27.344725595869313 28.651159725107426 22.482436699735537 68-69 21.30542654636445 28.050181371360512 28.56145793855736 22.08293414371768 70-71 21.877824645977704 27.60856056040976 27.91938931153962 22.594225482072915 72-73 21.751988890458033 26.973238243185993 28.403356399755214 22.871416466600763 74-75 21.45415004924914 28.13238075176432 28.040943276992003 22.372525921994537 76-77 22.043335274772467 26.891755943451173 28.269589415038503 22.795319366737854 78-79 21.635603946792642 27.07032518221214 28.835630296385872 22.45844057460934 80-81 21.633638734371672 27.97707198469897 28.192919040298115 22.19637024063124 82-83 21.726245797147826 27.482359197099353 28.307871127434947 22.48352387831787 84-85 21.802792442263367 27.214605317805905 27.936776490492825 23.0458257494379 86-87 21.916230501998182 27.299143670230098 28.349232903109677 22.43539292466204 88-89 22.272097781720756 26.746308064662255 28.169428386867523 22.812165766749462 90-91 21.791180018240123 26.67176605572063 28.758318378394282 22.778735547644967 92-93 21.86217569627106 27.569568261926197 28.359371781971387 22.208884259831354 94-95 21.6061950547834 27.67673676901531 27.98872556519283 22.72834261100846 96-97 21.13052647435415 27.301560028620926 28.129825073435267 23.43808842358967 98-99 21.901181942396345 27.24846210970186 27.54856696987065 23.301788978031148 100-101 22.458207437946516 26.65428891461704 28.12385282240985 22.76365082502659 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 606.0 1 354.5 2 100.5 3 108.0 4 111.0 5 118.5 6 181.5 7 309.5 8 487.0 9 587.0 10 535.5 11 517.0 12 587.5 13 648.5 14 707.5 15 800.5 16 862.5 17 881.0 18 884.0 19 883.5 20 980.0 21 1093.5 22 1229.0 23 1536.5 24 1878.5 25 2258.5 26 2793.0 27 3440.0 28 3946.5 29 4480.5 30 5194.5 31 5929.5 32 6793.5 33 7658.5 34 8634.5 35 9440.5 36 10440.5 37 11458.0 38 11777.5 39 12009.5 40 12456.5 41 12728.5 42 12782.5 43 13272.0 44 13935.0 45 14433.5 46 15487.0 47 18702.5 48 29507.5 49 29225.0 50 17985.0 51 14340.0 52 12623.5 53 11685.0 54 10956.5 55 10033.5 56 9155.5 57 8185.0 58 7293.0 59 6536.0 60 5537.5 61 4460.0 62 3514.5 63 2669.0 64 1943.5 65 1431.0 66 1020.0 67 734.5 68 543.0 69 367.5 70 245.5 71 168.5 72 128.0 73 107.5 74 79.5 75 56.5 76 47.5 77 32.5 78 19.5 79 9.0 80 4.0 81 5.5 82 6.5 83 4.0 84 3.0 85 2.5 86 1.0 87 2.0 88 3.0 89 1.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28871305104652634 2 0.09194832176765384 3 0.09639366047906713 4 0.1101976070039821 5 0.1130051893480326 6 0.08867280903292826 7 0.10107296438581796 8 0.09779745165109238 9 0.10364658153453092 10-11 0.09627667788139836 12-13 0.09885029503011132 14-15 0.10867683323428808 16-17 0.0965106430767359 18-19 0.10785795505060668 20-21 0.109729676613307 22-23 0.12189586677085916 24-25 0.13207335276804222 26-27 0.1333601613423987 28-29 0.12727706626362262 30-31 0.12084302339184023 32-33 0.11663164987576449 34-35 0.3818311987908679 36-37 0.5783619628744029 38-39 0.5588258690637181 40-41 0.5243160027514306 42-43 0.5521578609965981 44-45 0.573682658967652 46-47 0.56245232959145 48-49 0.5844450579531789 50-51 0.5769581717023775 52-53 0.5796487714487593 54-55 0.5701731810375888 56-57 0.5416294272064087 58-59 0.5341425409556074 60-61 0.4840739891533735 62-63 0.5597617298450682 64-65 0.5933357353760055 66-67 0.6067887341079141 68-69 0.6071396819009204 70-71 0.6040981343615324 72-73 0.5975471088920812 74-75 0.5927508223876615 76-77 0.5782449802767341 78-79 0.5521578609965981 80-81 0.5485314004688663 82-83 0.5626862947867876 84-85 0.572161885197958 86-87 0.5621013817984436 88-89 0.5773091194953839 90-91 0.5911130660202988 92-93 0.5993018478571128 94-95 0.598716934868769 96-97 0.5966112481107311 98-99 0.5995358130524503 100-101 0.5750864501396772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427414.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.25461305788623 #Duplication Level Percentage of deduplicated Percentage of total 1 86.04903853093144 61.31390944524663 2 9.489641286899571 13.523614359123409 3 2.294218762245135 4.904210105217594 4 0.8832707175204166 2.5174845280911526 5 0.39279908830240984 1.399437352323935 6 0.2340271145641176 1.0005306895991886 7 0.13501941924962962 0.6734529531753011 8 0.0942640959107156 0.5373401343495613 9 0.07275143111480666 0.4665487566143711 >10 0.31649230869260936 3.8911358010780606 >50 0.014141209600046799 0.6603468006545948 >100 0.016443266871003486 2.588428219550377 >500 0.004275249386460907 2.06586847578817 >1k 0.003617518711620767 4.4576923791876535 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3041 0.7114881590214639 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2684 0.6279625842859616 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2485 0.5814035104137909 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.4255826903189881 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1744 0.4080353006686725 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1567 0.3666234610939277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.33503815972335954 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1149 0.2688260094428353 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1093 0.255723958503933 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1006 0.23536898650956684 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1003 0.23466709092355423 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 898 0.21010074541311236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 0.20752712826439937 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 784 0.1834287131446326 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 761 0.17804751365186913 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 736 0.1721983837684306 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 698 0.16330770634560401 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 630 0.14739807306265118 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 612 0.14318669954657545 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 607 0.14201687356988774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 576 0.13476395251442397 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 567 0.13265826575638606 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 538 0.12587327509159738 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 522 0.1221298319661967 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 488 0.11417501532472028 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 450 0.10528433790189373 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 429 0.10037106879980534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 2.339651953375416E-4 12-13 0.0 0.0 0.0 0.0 2.339651953375416E-4 14-15 0.0 0.0 0.0 2.339651953375416E-4 2.339651953375416E-4 16-17 0.0 0.0 0.0 2.339651953375416E-4 2.339651953375416E-4 18-19 0.0 0.0 0.0 2.339651953375416E-4 2.339651953375416E-4 20-21 0.0 0.0 0.0 2.339651953375416E-4 2.339651953375416E-4 22-23 0.0 0.0 0.0 5.84912988343854E-4 2.339651953375416E-4 24-25 0.0 0.0 0.0 0.0015207737696940203 2.339651953375416E-4 26-27 0.0 0.0 0.0 0.002456634551044187 2.339651953375416E-4 28-29 0.0 0.0 0.0 0.007486886250801331 2.339651953375416E-4 30-31 0.0 0.0 0.0 0.019653076408353495 2.339651953375416E-4 32-33 0.0 0.0 0.0 0.04059296139106347 2.339651953375416E-4 34-35 0.0 0.0 0.0 0.07545377549635715 2.339651953375416E-4 36-37 0.0 0.0 0.0 0.12996766601000437 2.339651953375416E-4 38-39 0.0 0.0 0.0 0.20004024201359805 2.339651953375416E-4 40-41 0.0 0.0 0.0 0.27912047803768714 2.339651953375416E-4 42-43 0.0 0.0 0.0 0.3633479483592021 2.339651953375416E-4 44-45 0.0 0.0 0.0 0.4618472955963071 2.339651953375416E-4 46-47 0.0 0.0 0.0 0.5830412667811536 2.339651953375416E-4 48-49 0.0 0.0 0.0 0.70996738525177 2.339651953375416E-4 50-51 0.0 0.0 0.0 0.8475389201102445 2.339651953375416E-4 52-53 0.0 0.0 0.0 0.9727102996158292 2.339651953375416E-4 54-55 0.0 0.0 0.0 1.1210442334598305 2.339651953375416E-4 56-57 0.0 0.0 0.0 1.3027182076394315 2.339651953375416E-4 58-59 0.0 0.0 0.0 1.5033433626413735 2.339651953375416E-4 60-61 0.0 0.0 0.0 1.7411689837019846 2.339651953375416E-4 62-63 0.0 0.0 0.0 1.9889381255644412 2.339651953375416E-4 64-65 0.0 0.0 0.0 2.2354204588525413 2.339651953375416E-4 66-67 0.0 0.0 0.0 2.512201284936853 2.339651953375416E-4 68-69 0.0 0.0 0.0 2.835775150088673 2.339651953375416E-4 70-71 0.0 0.0 0.0 3.1880097516693415 2.339651953375416E-4 72-73 0.0 0.0 0.0 3.5542822649702632 2.339651953375416E-4 74-75 0.0 0.0 0.0 3.9276907167289794 2.339651953375416E-4 76-77 0.0 0.0 0.0 4.319465436321693 2.339651953375416E-4 78-79 0.0 0.0 0.0 4.778271184378612 2.339651953375416E-4 80-81 0.0 0.0 0.0 5.277552911228925 2.339651953375416E-4 82-83 0.0 0.0 0.0 5.771921368977151 2.339651953375416E-4 84-85 0.0 0.0 0.0 6.328992499075838 2.339651953375416E-4 86-87 0.0 0.0 0.0 6.921977286658837 2.339651953375416E-4 88-89 0.0 0.0 0.0 7.532275498696814 2.339651953375416E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGGT 90 5.8976E-7 36.773136 4 CTTTGCG 170 3.6379788E-12 33.37394 2 TTTGCGT 170 3.6379788E-12 33.37394 3 TCGCGCT 30 0.0039086575 31.735418 60-61 TTGCGTT 185 9.094947E-12 30.667944 4 CGCTCCG 45 8.8513165E-4 26.436827 48-49 CGCGGAA 45 8.857411E-4 26.43371 82-83 TACATGG 2405 0.0 23.787315 2 GTACATG 2490 0.0 23.784956 1 CCTATAC 100 0.0016972427 23.639874 3 GAGTACT 1510 0.0 22.543987 12-13 CAGTACC 210 3.6066922E-8 22.514166 4 ACATGGG 2425 0.0 22.031387 3 CTAGTAC 110 0.0029530022 21.490795 3 TAGACAG 225 7.483868E-8 21.013222 5 TAGTACC 115 0.003821005 20.556414 4 TAGTACT 140 4.9991027E-4 20.26275 4 TAGCCCT 375 0.0 20.172693 4 GTATAGC 165 6.435151E-5 20.10045 1 ATAGCCC 190 8.56861E-6 19.907263 3 >>END_MODULE