FastQCFastQC Report
Thu 26 May 2016
SRR938903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149021
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.3553459858436008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.28319760909504704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.1749315286665779No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19980.17388716133125504No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16700.1453411208324304No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13640.11870975378169762No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12260.10669952942548482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT35400.021.93627712-13
GTGTAGC6800.021.6417681
GTACATG40850.021.6152781
TACATGG40050.021.0546472
GTATAGG3401.8189894E-1220.9436451
GTATCAA86250.020.0346721
AGTACTT38050.019.72408912-13
GTACTTT39200.019.56864414-15
ACATGGG41300.019.2703553
TAGCCCT8700.018.513574
GGTATCA69000.018.3697031
GTATTAC2352.5415393E-618.1808681
TATCAAC98400.017.4762
CATGGGA20400.017.4165294
ATCAACG98300.017.3973943
CAACGCA99350.017.1658445
TCAACGC99800.017.135914
AACGCAG100500.016.9598086
ACTTTTT47650.016.6954116-17
GTATTAG2858.63487E-716.6569351
Produced by FastQC (version 0.11.5)