##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938903_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149021 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.720574297597693 33.0 31.0 34.0 30.0 34.0 2 31.803172439842267 33.0 31.0 34.0 30.0 34.0 3 31.84780086699895 34.0 31.0 34.0 30.0 34.0 4 35.386161784684525 37.0 35.0 37.0 33.0 37.0 5 35.42269114315578 37.0 35.0 37.0 33.0 37.0 6 35.502787155326146 37.0 35.0 37.0 33.0 37.0 7 35.42175817500289 37.0 35.0 37.0 33.0 37.0 8 35.4095173195268 37.0 35.0 37.0 33.0 37.0 9 37.00395902250698 39.0 37.0 39.0 33.0 39.0 10-11 36.88716655309172 39.0 37.0 39.0 33.0 39.0 12-13 36.773519369968 39.0 37.0 39.0 32.0 39.0 14-15 37.959771405396424 40.0 38.0 41.0 33.0 41.0 16-17 37.863617375139356 40.0 38.0 41.0 33.0 41.0 18-19 37.78251224303124 40.0 38.0 41.0 32.0 41.0 20-21 37.90083122936831 40.0 38.0 41.0 32.0 41.0 22-23 37.836372007126066 40.0 38.0 41.0 32.0 41.0 24-25 37.751439703887044 40.0 38.0 41.0 32.0 41.0 26-27 37.6244833645338 40.0 37.5 41.0 32.0 41.0 28-29 37.493693326753814 40.0 37.0 41.0 32.0 41.0 30-31 37.29507859299352 40.0 37.0 41.0 31.0 41.0 32-33 37.08894441441888 40.0 36.5 41.0 30.5 41.0 34-35 36.77550758428262 40.0 36.0 41.0 30.0 41.0 36-37 36.51196757935669 39.0 36.0 41.0 30.0 41.0 38-39 36.25979942925325 39.0 35.0 41.0 29.5 41.0 40-41 36.110910940705175 39.0 35.0 41.0 29.5 41.0 42-43 35.894675989385746 39.0 35.0 41.0 28.5 41.0 44-45 35.820460200466314 39.0 35.0 41.0 27.5 41.0 46-47 35.599489913587306 39.0 35.0 41.0 26.5 41.0 48-49 35.35216980368505 39.0 35.0 41.0 26.0 41.0 50-51 34.25407586110262 38.0 34.0 40.0 24.0 40.5 52-53 34.348766471631066 38.0 33.5 39.5 24.0 40.5 54-55 34.836750155132066 39.0 34.5 40.0 24.0 41.0 56-57 34.74062876135423 38.5 34.0 40.0 24.0 41.0 58-59 34.527233183727716 38.0 34.0 40.0 23.5 41.0 60-61 34.20923029257081 38.0 34.0 40.0 23.0 41.0 62-63 33.82327215951666 37.5 33.5 40.0 22.0 41.0 64-65 33.424772915377524 37.0 33.0 40.0 21.5 41.0 66-67 33.00345685587992 36.0 32.5 39.0 19.5 41.0 68-69 32.56582777860457 36.0 32.0 39.0 18.0 40.5 70-71 32.11685252053705 35.0 31.5 38.5 15.5 40.0 72-73 31.668582210420873 35.0 31.0 37.5 12.5 40.0 74-75 31.19511653834003 35.0 31.0 37.0 8.0 39.0 76-77 30.75871067630618 35.0 31.0 36.5 7.0 39.0 78-79 30.348219049086133 35.0 30.0 36.0 4.0 39.0 80-81 29.896082404063982 34.0 30.0 36.0 2.0 37.5 82-83 29.41477048722347 34.0 29.0 35.0 2.0 37.0 84-85 28.993692456447704 34.0 29.0 35.0 2.0 36.5 86-87 28.53099203582876 34.0 29.0 35.0 2.0 36.0 88-89 28.042454402486985 34.0 27.5 35.0 2.0 36.0 90-91 27.57658041062783 34.0 26.5 35.0 2.0 35.0 92-93 27.07205742975977 33.0 25.5 35.0 2.0 35.0 94-95 26.527730563671163 33.0 25.0 35.0 2.0 35.0 96-97 25.8536828308621 33.0 23.5 35.0 2.0 35.0 98-99 24.955663125391094 32.0 10.5 35.0 2.0 35.0 100-101 21.715617469132418 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.262835989648579 1101 2 0.34921506550565695 1101 3 0.48825905594186736 1101 4 0.33751144046835435 1101 5 0.24541168919360246 1101 6 0.3358362401411341 1101 7 0.4400389451276894 1101 8 0.3725951324988017 1101 9 0.4854207018151371 1101 10-11 0.3350996658246501 1101 12-13 0.3181641490054119 1101 14-15 0.5119263081207208 1101 16-17 0.5353848755417943 1101 18-19 0.724838347378352 1101 20-21 0.5576313671618962 1101 22-23 0.7666844139302924 1101 24-25 0.8268186680349672 1101 26-27 0.8186520586971042 1101 28-29 0.7970396438458849 1101 30-31 0.674758912564613 1101 32-33 0.6510136098155073 1101 34-35 0.5685214759644666 1101 36-37 0.6153434849530584 1101 38-39 0.7180749676498479 1101 40-41 0.6426478492531871 1101 42-43 0.8094347146952572 1101 44-45 0.766689358703843 1101 46-47 0.6681637015532402 1101 48-49 0.758291476880828 1101 50-51 0.7825566573314902 1101 52-53 0.7875075991863412 1101 54-55 1.0055774737539949 1101 56-57 0.748090108186048 1101 58-59 0.803739541588655 1101 60-61 0.7586278675957061 1101 62-63 0.7471302392561014 1101 64-65 0.6852030724916247 1101 66-67 0.83236000338362 1101 68-69 0.8226397420444798 1101 70-71 0.7157087869525611 1101 72-73 0.5854717845533521 1101 74-75 0.39194154177763707 1101 76-77 0.489676044084554 1101 78-79 0.4988796478077582 1101 80-81 0.6623443180782012 1101 82-83 0.5777669667171033 1101 84-85 0.4138968283722342 1101 86-87 0.3299034949181916 1101 88-89 0.29035779074881063 1101 90-91 0.08635664723041359 1101 92-93 0.2662883742309887 1101 94-95 0.3148361527537631 1101 96-97 0.13993022451625592 1101 98-99 0.06008368496276262 1101 100-101 0.570498853690669 1102 1 0.0876057335524898 1102 2 0.09569075531399918 1102 3 0.12834045934775418 1102 4 -0.13764516160269125 1102 5 -0.07822619131583508 1102 6 0.07344203053434484 1102 7 0.01226090554650483 1102 8 0.07460438637654221 1102 9 0.19689589419700582 1102 10-11 0.20268517518062623 1102 12-13 0.1329958355953167 1102 14-15 0.1460625925020551 1102 16-17 0.1476557757440773 1102 18-19 0.2535023296874215 1102 20-21 0.20238116164622255 1102 22-23 0.24847205995114052 1102 24-25 0.2962055593638695 1102 26-27 0.2930018699368375 1102 28-29 0.3706439262514678 1102 30-31 0.3237756662372817 1102 32-33 0.11602358452530837 1102 34-35 0.26906681246038744 1102 36-37 0.3450441214844062 1102 38-39 0.3476366738401069 1102 40-41 0.319707674822304 1102 42-43 0.4374077105465446 1102 44-45 0.5343807239038938 1102 46-47 0.5784340746580057 1102 48-49 0.6089679768834415 1102 50-51 0.321975523583113 1102 52-53 0.22013860124760498 1102 54-55 0.42825252860180996 1102 56-57 0.23125963985466313 1102 58-59 0.19117411255395922 1102 60-61 0.3638741460602759 1102 62-63 0.12406431192226108 1102 64-65 0.12136667518576161 1102 66-67 0.04642530413465096 1102 68-69 0.23572450177004356 1102 70-71 0.08717291377710978 1102 72-73 0.13689215530189003 1102 74-75 -0.007371049744470781 1102 76-77 -0.039052516398140114 1102 78-79 0.18596436215405987 1102 80-81 0.22027312151445955 1102 82-83 0.11830504253188323 1102 84-85 -0.03051096359528671 1102 86-87 0.16000704689227518 1102 88-89 0.25641410640692186 1102 90-91 0.17521883217183287 1102 92-93 0.07285885432607486 1102 94-95 0.1994497920131053 1102 96-97 -0.07382440285895697 1102 98-99 0.016895722334329122 1102 100-101 0.25716424624742373 1103 1 0.17072227539981455 1103 2 0.0837720411721854 1103 3 0.24393981116310925 1103 4 0.20347777449856608 1103 5 -0.02467387668350085 1103 6 0.1947883275605804 1103 7 0.0683726736015089 1103 8 0.06765041624559132 1103 9 0.3505559476284432 1103 10-11 0.24338985700079974 1103 12-13 0.12740655691971625 1103 14-15 0.16866014867076018 1103 16-17 0.20952587603751027 1103 18-19 0.25284188208964054 1103 20-21 0.36454776261850697 1103 22-23 0.28111034664145507 1103 24-25 0.34156315582512775 1103 26-27 0.310117886229051 1103 28-29 0.25212423983268906 1103 30-31 0.41982173990258786 1103 32-33 0.3891729735674829 1103 34-35 0.25634180917567306 1103 36-37 0.32564544413145313 1103 38-39 0.4934444958520956 1103 40-41 0.3380596215660532 1103 42-43 0.14005738426440928 1103 44-45 0.250801693553079 1103 46-47 0.3702336629731633 1103 48-49 0.3546347749388872 1103 50-51 0.3070409989915177 1103 52-53 0.2532704717858607 1103 54-55 0.3809276041503509 1103 56-57 0.13462932408362605 1103 58-59 0.18318260902836414 1103 60-61 0.3409034457580873 1103 62-63 0.16659485981346478 1103 64-65 -0.13896530046351785 1103 66-67 -0.03333907198763342 1103 68-69 -0.10659232037334476 1103 70-71 -0.021583710407178103 1103 72-73 -0.06003115744720233 1103 74-75 -0.055542993860441925 1103 76-77 -0.13724811049440788 1103 78-79 -0.04441797919398738 1103 80-81 -0.04104383201771 1103 82-83 -0.05792628931238042 1103 84-85 -0.1263135300561089 1103 86-87 -0.06261196944372927 1103 88-89 0.03549088125035027 1103 90-91 0.25453648644415594 1103 92-93 0.10819323876964404 1103 94-95 -0.1865406604022617 1103 96-97 -0.20357345029030682 1103 98-99 -0.22199360451716288 1103 100-101 -0.03347079951966592 1104 1 0.022571091036006408 1104 2 0.20558073082140638 1104 3 0.1564772405111654 1104 4 0.2537716002741206 1104 5 2.0250938889887493E-4 1104 6 0.1668597777051204 1104 7 0.13100804487475415 1104 8 0.1525544159004255 1104 9 0.14451633939614794 1104 10-11 0.2615634774003368 1104 12-13 0.2639980053384079 1104 14-15 0.383908886643745 1104 16-17 0.41982401643532086 1104 18-19 0.44796074993276846 1104 20-21 0.4050189193687288 1104 22-23 0.46902854140350314 1104 24-25 0.3190149399214306 1104 26-27 0.32232222673903976 1104 28-29 0.3687502052294249 1104 30-31 0.4419069968383411 1104 32-33 0.3172987016599862 1104 34-35 0.653263326196857 1104 36-37 0.378463755388303 1104 38-39 0.3191932985444268 1104 40-41 0.4007804223599436 1104 42-43 0.31706907689072494 1104 44-45 0.3929464922759536 1104 46-47 0.5436797517680958 1104 48-49 0.3869021089337963 1104 50-51 0.34107258202642043 1104 52-53 0.5070565528144684 1104 54-55 0.7044414325146775 1104 56-57 0.7508799173441858 1104 58-59 0.6876452228265251 1104 60-61 0.7901552902590936 1104 62-63 0.691106228843104 1104 64-65 0.7330970889742829 1104 66-67 0.68750227935206 1104 68-69 0.7005561457784424 1104 70-71 0.6497093818825874 1104 72-73 0.49713327280692354 1104 74-75 0.505683221784377 1104 76-77 0.3094943497032041 1104 78-79 0.4463413943228609 1104 80-81 0.7078818270408398 1104 82-83 0.7995039216726489 1104 84-85 0.6026198953224977 1104 86-87 0.5106887165812104 1104 88-89 0.6084278446192037 1104 90-91 0.7256871961995195 1104 92-93 0.5815823745508055 1104 94-95 0.6885652635740946 1104 96-97 0.4961996005790894 1104 98-99 0.532780093545572 1104 100-101 0.7892448237275183 1105 1 0.3467750723828473 1105 2 0.26090172899954567 1105 3 0.38148852266530753 1105 4 0.3685186293068625 1105 5 0.3214480592058777 1105 6 0.3555258092282969 1105 7 0.48886293130107106 1105 8 0.4431542922145866 1105 9 0.46140447056998823 1105 10-11 0.44044412056668847 1105 12-13 0.4732309859999617 1105 14-15 0.5917336873256929 1105 16-17 0.5329639652895537 1105 18-19 0.5759154207445789 1105 20-21 0.5513275656773757 1105 22-23 0.5757510338402838 1105 24-25 0.6149132501054595 1105 26-27 0.4212199184938754 1105 28-29 0.4858472651836223 1105 30-31 0.5458808222407399 1105 32-33 0.40469361048346286 1105 34-35 0.5911312497790675 1105 36-37 0.5550252890926899 1105 38-39 0.7413430921227899 1105 40-41 0.6869953014319705 1105 42-43 0.6286913670844356 1105 44-45 0.5885263949987092 1105 46-47 0.39339310148144335 1105 48-49 0.5899039976499694 1105 50-51 0.47896641277738894 1105 52-53 0.6659878403171859 1105 54-55 0.6333830828848974 1105 56-57 0.38211727715297883 1105 58-59 0.3849466986994301 1105 60-61 0.4549491193386359 1105 62-63 0.44551796896913487 1105 64-65 0.5568046555389969 1105 66-67 0.44033786075906534 1105 68-69 0.6030808411602777 1105 70-71 0.4022566415727056 1105 72-73 0.23195501477152547 1105 74-75 0.20588797198912445 1105 76-77 0.25797501961244507 1105 78-79 0.46574753515757195 1105 80-81 0.5566934401381687 1105 82-83 0.7119961913077759 1105 84-85 0.8492594848192319 1105 86-87 0.6159570647066985 1105 88-89 0.7581370157569474 1105 90-91 0.8060428765551997 1105 92-93 0.7171938673051557 1105 94-95 0.8695393731196326 1105 96-97 0.8301914774281087 1105 98-99 0.9253372146741228 1105 100-101 1.235766577392127 1106 1 0.26839669400446553 1106 2 0.34820327809984875 1106 3 0.3734550216665369 1106 4 0.400688733516148 1106 5 0.37075613843381205 1106 6 0.47020094741188956 1106 7 0.3660885095061701 1106 8 0.33873161486225456 1106 9 0.37035010364741083 1106 10-11 0.45261390388518663 1106 12-13 0.2974237450992163 1106 14-15 0.3103433477430073 1106 16-17 0.4474798614172073 1106 18-19 0.4838394177602936 1106 20-21 0.5493621095712271 1106 22-23 0.5079251491844801 1106 24-25 0.619914333111879 1106 26-27 0.6765475078820131 1106 28-29 0.4765807413133629 1106 30-31 0.5795960156175539 1106 32-33 0.628543016434385 1106 34-35 0.6513572371199245 1106 36-37 0.5812200056257879 1106 38-39 0.6546335865352759 1106 40-41 0.5431427667958175 1106 42-43 0.2225130159817823 1106 44-45 0.3200033750954603 1106 46-47 0.15616254946905883 1106 48-49 0.06349227871821483 1106 50-51 0.311917886067242 1106 52-53 0.11729747586547035 1106 54-55 0.10574195110307727 1106 56-57 0.24468295948713603 1106 58-59 0.3069325313709257 1106 60-61 0.48711521241301625 1106 62-63 0.5216902231467273 1106 64-65 0.22998580641382205 1106 66-67 0.12611918972828562 1106 68-69 0.17051965129721935 1106 70-71 0.14668276850106565 1106 72-73 0.07618620054322989 1106 74-75 0.1671015007449519 1106 76-77 -0.128865248973149 1106 78-79 0.08792189116986293 1106 80-81 0.11075471423506045 1106 82-83 0.3400757401353225 1106 84-85 0.49091151084347473 1106 86-87 0.5498459402251399 1106 88-89 0.35741140145163897 1106 90-91 0.2789420446458202 1106 92-93 0.20393305529100303 1106 94-95 0.4214976091829463 1106 96-97 0.5325668715221141 1106 98-99 0.5855190795309646 1106 100-101 1.1986744095341528 1107 1 0.2526210585969295 1107 2 0.34303136082243313 1107 3 0.27350832511455536 1107 4 0.2611187666922774 1107 5 0.39510559053211836 1107 6 0.22778264099432732 1107 7 0.27210664828712083 1107 8 0.17420892265572263 1107 9 0.2993417943968879 1107 10-11 0.37398195287753566 1107 12-13 0.3551916495557137 1107 14-15 0.42706424136790133 1107 16-17 0.40885164087048054 1107 18-19 0.31676744717942995 1107 20-21 0.32056349748999224 1107 22-23 0.4035186787858436 1107 24-25 0.3873532624227636 1107 26-27 0.4174256334112485 1107 28-29 0.32565745223308085 1107 30-31 0.44241065619388564 1107 32-33 0.5613287732535994 1107 34-35 0.565185540704519 1107 36-37 0.32050878310379716 1107 38-39 0.6117254815235853 1107 40-41 0.6237620857447652 1107 42-43 0.6058849936645672 1107 44-45 0.48139419706896547 1107 46-47 0.6291709476887348 1107 48-49 0.9573973455114242 1107 50-51 0.7107158659992407 1107 52-53 0.7925261226836895 1107 54-55 0.7511764678562045 1107 56-57 0.7301587454398444 1107 58-59 0.6839147289558056 1107 60-61 0.6182537912322701 1107 62-63 0.5459214399247472 1107 64-65 0.7355584750903645 1107 66-67 0.7300031614673159 1107 68-69 0.7420781645134298 1107 70-71 0.6172304672827309 1107 72-73 0.6496196996141244 1107 74-75 0.631609442802624 1107 76-77 0.7281718597479347 1107 78-79 0.8384473164463095 1107 80-81 0.7876335827347489 1107 82-83 0.5247335561923769 1107 84-85 0.5734104982340504 1107 86-87 0.7168788892481572 1107 88-89 0.7662297272238909 1107 90-91 0.7657636841287676 1107 92-93 0.5712929269256861 1107 94-95 0.5216206831764048 1107 96-97 0.5837338892527306 1107 98-99 0.5297268365484094 1107 100-101 0.5445517429257585 1108 1 0.21181042313248 1108 2 0.12209279019061015 1108 3 0.14905609023287525 1108 4 0.20460647968042878 1108 5 0.23118581180077058 1108 6 0.4126853806735795 1108 7 0.38147186676714995 1108 8 0.22954885003771608 1108 9 0.4552770072996708 1108 10-11 0.37798277239105715 1108 12-13 0.39618724467050725 1108 14-15 0.46646918348975674 1108 16-17 0.3906032372145418 1108 18-19 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-1.8046432847281686 2316 100-101 -2.093475971309278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3740.0 3 1136.0 4 1111.0 5 1303.0 6 1484.0 7 1819.0 8 2248.0 9 2711.0 10 3358.0 11 4258.0 12 5499.0 13 7408.0 14 6831.0 15 6872.0 16 8477.0 17 10870.0 18 9598.0 19 7496.0 20 7663.0 21 8075.0 22 8712.0 23 9802.0 24 11173.0 25 12573.0 26 14320.0 27 16717.0 28 19648.0 29 23434.0 30 28222.0 31 34033.0 32 41706.0 33 53178.0 34 71291.0 35 101567.0 36 152802.0 37 224991.0 38 194155.0 39 28733.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26603207200222 19.00370034698393 14.806539433405105 22.923728147608742 2 15.055750910454483 20.308121663372685 40.75961649854145 23.87651092763139 3 19.23151638269644 24.547431985218857 30.204826172591293 26.01622545949341 4 12.824404072223954 16.813692095125425 37.558564790212166 32.80333904243846 5 13.793506047673898 37.17921015165799 34.39349872877738 14.633785071890731 6 31.384822234065158 36.16335935295778 17.761615654966544 14.690202758010527 7 27.784347704928024 33.079689636254386 21.148711607290586 17.987251051527 8 25.371388761892323 35.34661354581674 19.97282999150937 19.309167700781575 9 25.92453576177915 17.411535001510956 21.01658220693751 35.647347029772384 10-11 24.676027563673287 26.48462148715997 27.526532169084835 21.312818780081912 12-13 25.87809512530283 23.9923263011413 27.873152329714767 22.256426243841098 14-15 22.87032183119319 25.360947642128036 26.57049233432547 25.198238192353312 16-17 21.904785955843582 28.432110607441373 27.243665800350854 22.41943763636419 18-19 21.693168462942264 27.500412582391288 29.25320192239445 21.553217032272 20-21 22.802154974582553 26.52994573372687 28.968836301532715 21.69906299015787 22-23 22.370328173288318 26.333650392142516 28.948253215011167 22.347768219558 24-25 22.251486771717058 26.50029574278479 29.133277640190357 22.114939845307795 26-27 22.277055451246117 26.422822121266364 29.275892150573178 22.024230276914338 28-29 21.980042611532987 26.783334465692143 29.290681732265085 21.94594119050979 30-31 22.1983957175003 26.62090758369483 28.971195817141933 22.209500881662937 32-33 22.06486107046807 26.711667224056967 28.934478770397 22.288992935077964 34-35 22.47208816538212 26.64462095564883 28.74040411316216 22.142886765806885 36-37 22.526382815516868 26.47911481614137 28.974281264800773 22.020221103540987 38-39 22.360378793840667 26.586701992777385 29.078285809221516 21.974633404160436 40-41 22.431684840622005 26.68118193509131 28.918051532845567 21.96908169144112 42-43 22.11194035071763 26.85896785613091 28.592923031717106 22.436168761434352 44-45 22.356429807115408 26.807888554791155 28.47763381838727 22.35804781970617 46-47 22.507428226605185 26.94298139171843 28.098352125270836 22.45123825640555 48-49 22.576888138970492 26.97873710184822 28.082709118112586 22.361665641068704 50-51 22.424958070109838 27.25707450728279 28.06138492185843 22.25658250074894 52-53 22.609114354301138 27.31091867334858 27.81949724674829 22.260469725601983 54-55 22.26876002223203 27.056473459970764 28.063605287934053 22.611161229863157 56-57 22.399529228039786 27.362920674837266 27.70887336068136 22.52867673644159 58-59 22.551284511507077 27.041206393029793 27.84464079095013 22.562868304513 60-61 22.510045635763337 26.927416239759914 28.208267571341928 22.354270553134814 62-63 22.394058579052427 26.94710744885756 28.222624010983843 22.43620996110617 64-65 22.35337184723722 27.154532775087286 28.06393500483501 22.42816037284048 66-67 22.26380481608669 27.100055856562793 27.91511552073232 22.72102380661819 68-69 22.129447558887026 27.24798715629641 27.992012056283066 22.630553228533497 70-71 22.42570987370788 26.968376662860845 27.782210615187424 22.82370284824385 72-73 22.224661896180695 26.9254859422293 27.895620833843626 22.95423132774638 74-75 22.190690433897917 27.463171886980703 27.626760048971473 22.71937763014991 76-77 22.451785393345393 26.968301216499686 27.665561632442092 22.91435175771283 78-79 22.280859911931252 27.083938121036823 27.854146191323935 22.781055775707994 80-81 22.385957118984805 27.14402889864575 27.690207007274502 22.779806975094942 82-83 22.338937901409043 27.098958260455873 27.76946003050359 22.792643807631492 84-85 22.444449788905917 27.036833785547653 27.578485238718876 22.940231186827553 86-87 22.363641531421425 26.995467653740114 27.797821937605356 22.843068877233105 88-89 22.478041740917686 26.890902928861145 27.63607342378292 22.994981906438248 90-91 22.38318731884866 26.84689625733189 27.76164627887214 23.008270144947314 92-93 22.377781733710243 27.04300706075627 27.683824381675365 22.89538682385812 94-95 22.337058815552982 26.916598101521906 27.665675041893635 23.080668041031483 96-97 22.219627108384202 26.794827384107755 27.723601131586268 23.261944375921775 98-99 22.296021446632956 26.88247435847527 27.514605904122636 23.306898290769137 100-101 22.689991017882896 26.084741508313485 28.00089733711276 23.224370136690855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1005.0 1 610.5 2 204.5 3 196.5 4 181.5 5 210.0 6 342.0 7 696.5 8 1180.0 9 1405.0 10 1318.0 11 1230.5 12 1326.0 13 1441.5 14 1550.0 15 1701.5 16 1850.5 17 1945.0 18 2007.5 19 2090.0 20 2267.0 21 2556.0 22 2998.0 23 3578.0 24 4245.0 25 5275.5 26 6697.5 27 8227.5 28 9744.0 29 11443.5 30 13298.0 31 15462.5 32 17560.5 33 19417.5 34 21597.0 35 23797.5 36 26524.0 37 29322.0 38 30634.0 39 31025.5 40 32241.0 41 33797.0 42 34924.5 43 36469.5 44 38343.5 45 39849.5 46 41533.0 47 44782.0 48 51955.0 49 51815.0 50 44458.5 51 41318.0 52 40277.5 53 39835.0 54 38605.5 55 35760.5 56 32463.5 57 29201.0 58 26337.0 59 23298.5 60 19524.0 61 16087.0 62 12875.0 63 10045.5 64 7269.5 65 5095.0 66 3677.5 67 2727.5 68 2032.5 69 1493.0 70 1098.0 71 725.0 72 484.5 73 328.5 74 211.0 75 137.5 76 87.5 77 48.5 78 34.0 79 21.5 80 10.5 81 7.0 82 6.5 83 8.0 84 10.5 85 9.0 86 6.5 87 7.0 88 6.0 89 4.0 90 3.5 91 3.5 92 4.0 93 3.5 94 2.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3240149657839152 2 0.08424563171604349 3 0.09329681528884154 4 0.09982411113460937 5 0.11374900893891407 6 0.05717911160892621 7 0.06013815239234095 8 0.06057330544872548 9 0.06457671356746308 10-11 0.06074736667127929 12-13 0.059441907502125725 14-15 0.057005050386372394 16-17 0.05844105547244133 18-19 0.06662193293247033 20-21 0.06910230535386211 22-23 0.0843326623273204 24-25 0.0926005703986263 26-27 0.08655194291488146 28-29 0.08485484599498182 30-31 0.07889324912251386 32-33 0.07075588696812331 34-35 0.3197069505257084 36-37 0.4969883056967627 38-39 0.4691820253937918 40-41 0.44977419907904204 42-43 0.48188849464021977 44-45 0.4911137394355717 46-47 0.48550026500821136 48-49 0.5035156015425306 50-51 0.49986031586890056 52-53 0.4992511015899623 54-55 0.48959070373822583 56-57 0.46713680602878455 58-59 0.45094911233128027 60-61 0.41278618928635763 62-63 0.4807135813879816 64-65 0.5045599688778534 66-67 0.5147425503972511 68-69 0.5264046523083564 70-71 0.525316769667395 72-73 0.511827024919475 74-75 0.514046305507036 76-77 0.4888944588480106 78-79 0.46726735194569985 80-81 0.47327246412380625 82-83 0.48293286197554264 84-85 0.4850651119518268 86-87 0.4850651119518268 88-89 0.49246271391036367 90-91 0.505865428047007 92-93 0.5110002341123443 94-95 0.5176145605693891 96-97 0.5088679841360602 98-99 0.5138722442844822 100-101 0.49067858637918715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1149021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.82904740958828 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67916679551371 54.58540910153054 2 11.111485737500004 14.851400142847035 3 3.2620706147206064 6.540032152937647 4 1.426722548476321 3.813860353298107 5 0.6997300625670696 2.3381146762604437 6 0.4419432386498545 1.772078738884687 7 0.2920488614967176 1.3662143047616295 8 0.20134514802387887 1.0764563554382698 9 0.1619761231374635 0.9742239011137372 >10 0.6809881121736843 7.825253974922935 >50 0.027798736664311884 1.2632138068686243 >100 0.012508908465819856 1.6232375815747744 >500 0.0011727066761920595 0.5196859472474823 >1k 0.0010424059343929416 1.4508189623140588 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4083 0.3553459858436008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3254 0.28319760909504704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2010 0.1749315286665779 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1998 0.17388716133125504 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1670 0.1453411208324304 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1364 0.11870975378169762 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1226 0.10669952942548482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.3054591691535666E-4 0.0 0.0 8.703061127690443E-5 0.0 14-15 1.7406122255380886E-4 0.0 0.0 8.703061127690443E-5 0.0 16-17 1.7406122255380886E-4 0.0 0.0 8.703061127690443E-5 0.0 18-19 1.7406122255380886E-4 0.0 0.0 8.703061127690443E-5 0.0 20-21 1.7406122255380886E-4 0.0 0.0 2.6109183383071327E-4 0.0 22-23 3.4812244510761773E-4 0.0 0.0 3.046071394691655E-4 0.0 24-25 3.4812244510761773E-4 0.0 0.0 5.221836676614265E-4 0.0 26-27 3.4812244510761773E-4 0.0 0.0 8.703061127690443E-4 0.0 28-29 4.3515305638452213E-4 0.0 0.0 0.0031331020059685594 0.0 30-31 4.3515305638452213E-4 0.0 0.0 0.00826790807130592 0.0 32-33 4.3515305638452213E-4 0.0 0.0 0.01927728039783433 0.0 34-35 4.7866836202297433E-4 0.0 0.0 0.03476872920512332 0.0 36-37 6.092142789383309E-4 0.0 0.0 0.06135658095021762 0.0 38-39 6.962448902152355E-4 0.0 0.0 0.09886677441056343 0.0 40-41 7.397601958536877E-4 0.0 0.0 0.1379435188738935 0.0 42-43 8.267908071305921E-4 0.0 0.0 0.18185046226309182 0.0 44-45 8.703061127690443E-4 0.0 0.0 0.2387684820381873 0.0 46-47 0.001044367335322853 0.0 0.0 0.3059561139439575 0.0 48-49 0.001044367335322853 0.0 0.0 0.3798886182236878 0.0 50-51 0.001044367335322853 0.0 0.0 0.45852077551237097 0.0 52-53 0.001044367335322853 0.0 0.0 0.5410257950028764 0.0 54-55 0.001044367335322853 0.0 0.0 0.6328430899000106 0.0 56-57 0.0011313979465997575 0.0 0.0 0.7454607008923249 0.0 58-59 0.0011313979465997575 0.0 0.0 0.8740919443595896 0.0 60-61 0.0011313979465997575 0.0 0.0 1.014341774432321 0.0 62-63 0.0011313979465997575 0.0 0.0 1.1574636146771904 0.0 64-65 0.0011313979465997575 0.0 0.0 1.3144233221150876 0.0 66-67 0.0013054591691535663 0.0 0.0 1.4954034782654102 0.0 68-69 0.0013054591691535663 0.0 0.0 1.7037982769679578 0.0 70-71 0.0013054591691535663 0.0 0.0 1.9334285448220703 0.0 72-73 0.0013489744747920186 0.0 0.0 2.1705434452459964 0.0 74-75 0.001392489780430471 0.0 0.0 2.4222794883644427 0.0 76-77 0.001392489780430471 0.0 0.0 2.6944242098273223 0.0 78-79 0.001392489780430471 0.0 0.0 3.0181345684717686 0.0 80-81 0.0015665510029842797 0.0 0.0 3.3632109421846943 0.0 82-83 0.0016535816142611841 0.0 0.0 3.712029632182528 0.0 84-85 0.0016535816142611841 0.0 0.0 4.087479689231094 0.0 86-87 0.0016535816142611841 0.0 0.0 4.500831577490751 0.0 88-89 0.0016535816142611841 0.0 0.0 4.948560557204786 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 3540 0.0 21.936277 12-13 GTGTAGC 680 0.0 21.641768 1 GTACATG 4085 0.0 21.615278 1 TACATGG 4005 0.0 21.054647 2 GTATAGG 340 1.8189894E-12 20.943645 1 GTATCAA 8625 0.0 20.034672 1 AGTACTT 3805 0.0 19.724089 12-13 GTACTTT 3920 0.0 19.568644 14-15 ACATGGG 4130 0.0 19.270355 3 TAGCCCT 870 0.0 18.51357 4 GGTATCA 6900 0.0 18.369703 1 GTATTAC 235 2.5415393E-6 18.180868 1 TATCAAC 9840 0.0 17.476 2 CATGGGA 2040 0.0 17.416529 4 ATCAACG 9830 0.0 17.397394 3 CAACGCA 9935 0.0 17.165844 5 TCAACGC 9980 0.0 17.13591 4 AACGCAG 10050 0.0 16.959808 6 ACTTTTT 4765 0.0 16.69541 16-17 GTATTAG 285 8.63487E-7 16.656935 1 >>END_MODULE