FastQCFastQC Report
Thu 26 May 2016
SRR938903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149021
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.33898423092354274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.3055644761932114No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23770.20687176300520183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22370.1946874774264352No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16960.1476039167256299No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14460.1258462639064038No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11500.1000852029684401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3800.023.8350371
GTGTACG1201.6698489E-423.8350371
GTACATG38950.022.3969821
TACATGG38450.022.2143252
AGTACTT39300.020.46694412-13
GAGTACT38300.020.3818212-13
ACATGGG40600.020.2198073
GCACCGT1656.3718086E-520.1312626
CCGTTGC1200.004791285419.7717769
GTATCAA84850.019.4950121
CATGGGG22100.018.6803044
GTACTTT40950.018.59931614-15
GCTATAT3609.094947E-1118.5383631
GGTATCA67500.018.5030521
TGCACCG1559.6897315E-418.3686185
GTGTAGC5950.017.625911
GTATAGG3301.15778676E-817.3345741
TATCAAC98600.016.7478562
CAACGCA98600.016.699735
TCAACGC98450.016.6287774
Produced by FastQC (version 0.11.5)