##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938903_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149021 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.193225363156984 33.0 31.0 34.0 30.0 34.0 2 32.43599029086501 34.0 31.0 34.0 31.0 34.0 3 32.52405395549777 34.0 31.0 34.0 31.0 34.0 4 36.074596547843775 37.0 35.0 37.0 35.0 37.0 5 35.9961219159615 37.0 35.0 37.0 35.0 37.0 6 36.03352941330054 37.0 35.0 37.0 35.0 37.0 7 35.96813896351764 37.0 35.0 37.0 35.0 37.0 8 35.939677342711754 37.0 35.0 37.0 35.0 37.0 9 37.62278322154251 39.0 37.0 39.0 35.0 39.0 10-11 37.52859390733503 39.0 37.0 39.0 35.0 39.0 12-13 37.43706468376122 39.0 37.0 39.0 34.0 39.0 14-15 38.756471813831084 40.0 38.0 41.0 34.5 41.0 16-17 38.845357482587346 40.0 38.0 41.0 35.0 41.0 18-19 38.885855001779774 40.0 38.0 41.0 35.0 41.0 20-21 38.86228363102154 40.0 38.0 41.0 34.5 41.0 22-23 38.78209884762768 40.0 38.0 41.0 34.0 41.0 24-25 38.710156298274796 40.0 38.0 41.0 34.0 41.0 26-27 38.544823810879 40.0 38.0 41.0 34.0 41.0 28-29 38.383887239658804 40.0 38.0 41.0 34.0 41.0 30-31 38.19142295919744 40.0 38.0 41.0 33.5 41.0 32-33 38.096192758879084 40.0 38.0 41.0 33.0 41.0 34-35 37.99873675067732 40.0 37.0 41.0 33.0 41.0 36-37 37.86336194029526 40.0 37.0 41.0 33.0 41.0 38-39 37.71302743814081 40.0 37.0 41.0 33.0 41.0 40-41 37.539378740684455 40.0 37.0 41.0 32.0 41.0 42-43 37.35095181028023 40.0 37.0 41.0 32.0 41.0 44-45 37.09868662104522 40.0 36.0 41.0 31.0 41.0 46-47 36.78434119132723 40.0 36.0 41.0 30.5 41.0 48-49 36.44325212506995 39.0 36.0 41.0 30.0 41.0 50-51 36.171082164729796 39.0 35.0 41.0 29.5 41.0 52-53 35.897100227062865 39.0 35.0 40.0 28.5 41.0 54-55 35.59074246684787 38.5 35.0 40.0 28.0 41.0 56-57 35.440324850459646 38.0 35.0 40.0 28.0 41.0 58-59 35.14835499090095 38.0 34.0 40.0 27.0 41.0 60-61 34.7726364444166 38.0 34.0 40.0 26.5 41.0 62-63 34.3369263921199 37.0 34.0 40.0 26.0 41.0 64-65 33.908879385146136 36.5 33.0 39.5 25.5 41.0 66-67 33.43545244168732 36.0 33.0 39.0 24.5 40.0 68-69 32.9341017266003 35.5 32.0 39.0 22.5 40.0 70-71 32.3954666624892 35.0 31.5 38.0 22.0 40.0 72-73 31.858631391419305 35.0 31.0 37.0 20.5 39.5 74-75 31.31640979581748 34.5 31.0 37.0 18.5 39.0 76-77 29.425042275119427 32.5 28.5 35.0 15.0 37.0 78-79 30.38154089437878 34.0 30.0 36.0 17.0 38.0 80-81 30.350178543299034 34.0 30.0 35.0 14.5 37.0 82-83 29.92211021382551 34.0 30.0 35.0 8.5 37.0 84-85 29.43911773588124 34.0 29.0 35.0 4.5 36.0 86-87 28.713208026659217 34.0 29.0 35.0 2.0 36.0 88-89 27.985284429092246 33.0 27.0 35.0 2.0 35.0 90-91 27.38167579182626 33.0 26.0 35.0 2.0 35.0 92-93 26.9358366818361 32.0 25.5 34.0 2.0 35.0 94-95 26.33120587004067 32.0 25.0 34.0 2.0 35.0 96-97 25.419151173042096 32.0 22.0 34.0 2.0 35.0 98-99 24.125706579775304 31.5 9.5 34.0 2.0 35.0 100-101 18.434388492464453 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17197564577978142 1101 2 0.25243270343989366 1101 3 0.3242909928540314 1101 4 0.15712310248330397 1101 5 0.18094056571538886 1101 6 0.22374014661570385 1101 7 0.26994139037488907 1101 8 0.2983285989714375 1101 9 0.374695433586993 1101 10-11 0.37847161273048613 1101 12-13 0.32163462596611225 1101 14-15 0.3349027272454279 1101 16-17 0.17645450551957254 1101 18-19 0.33349894939719604 1101 20-21 0.34140720269811453 1101 22-23 0.44173860705414825 1101 24-25 0.3222558165865834 1101 26-27 0.34905416436365755 1101 28-29 0.35182091838707663 1101 30-31 0.3826203333975968 1101 32-33 0.41019355951819136 1101 34-35 0.561077226650248 1101 36-37 0.794841582086363 1101 38-39 0.7991123214581535 1101 40-41 0.6904363067635444 1101 42-43 0.7056392493506891 1101 44-45 0.7812464762039824 1101 46-47 0.5842821207317641 1101 48-49 0.6968966111116188 1101 50-51 0.6633995930303769 1101 52-53 0.651821752393424 1101 54-55 0.6088937019426695 1101 56-57 0.6950290905452903 1101 58-59 0.7959479594445256 1101 60-61 0.7120970552333503 1101 62-63 0.9536060006001392 1101 64-65 0.8050132033055704 1101 66-67 0.6302633998221623 1101 68-69 0.9136355083702554 1101 70-71 0.8686111521385058 1101 72-73 0.9271188290368286 1101 74-75 1.0175607886255307 1101 76-77 0.7336918890956987 1101 78-79 1.1146599317370907 1101 80-81 0.9971540544901174 1101 82-83 0.8778851535775978 1101 84-85 1.2642568648980905 1101 86-87 1.3159227976946148 1101 88-89 1.2122550497144644 1101 90-91 1.1795278124915356 1101 92-93 1.4304587108368807 1101 94-95 1.3038550625261038 1101 96-97 1.1886279185993516 1101 98-99 1.5676969891800248 1101 100-101 1.639428489459224 1102 1 0.12145718776694281 1102 2 0.06490728669250245 1102 3 0.06784321128883164 1102 4 0.10448316419908821 1102 5 0.09931026354189498 1102 6 0.08491627223944676 1102 7 0.15715648281766903 1102 8 0.0875262413444915 1102 9 0.10833577504845238 1102 10-11 0.2030406406893448 1102 12-13 0.11271201679845433 1102 14-15 0.2122705208885023 1102 16-17 0.1959693314464772 1102 18-19 0.07725406547210412 1102 20-21 0.14346855545377224 1102 22-23 0.1465797011131258 1102 24-25 0.156868495900099 1102 26-27 0.309524031664246 1102 28-29 0.2914145820464924 1102 30-31 0.20390241958676825 1102 32-33 0.2603983770499454 1102 34-35 0.2240891177609754 1102 36-37 0.21287748056947464 1102 38-39 0.2794831747103572 1102 40-41 0.18722790601582773 1102 42-43 0.15096291690225172 1102 44-45 0.07390777764616274 1102 46-47 0.20074187543942656 1102 48-49 0.16692535712565615 1102 50-51 -0.007913211425162103 1102 52-53 -0.024219657924071214 1102 54-55 0.16010637532197336 1102 56-57 0.014806679824616253 1102 58-59 0.10037645133472495 1102 60-61 0.1575612239464519 1102 62-63 0.1265995829259694 1102 64-65 0.1615405731063717 1102 66-67 0.2212492467300038 1102 68-69 0.19206866734251804 1102 70-71 0.23910355198561462 1102 72-73 0.3795508954162159 1102 74-75 0.41533484879493443 1102 76-77 -0.08914487298958207 1102 78-79 0.01761420864481167 1102 80-81 0.07348021964617857 1102 82-83 0.026370882673901974 1102 84-85 0.4814616248330914 1102 86-87 0.5150020351136533 1102 88-89 0.17963041820275905 1102 90-91 0.537281294913182 1102 92-93 0.8637071325986767 1102 94-95 0.9024004827991092 1102 96-97 0.6102710316862066 1102 98-99 0.9239315755044579 1102 100-101 1.139036657027365 1103 1 0.0775514373180286 1103 2 0.03642132339376758 1103 3 0.0456376316453273 1103 4 0.10406622380580188 1103 5 0.06724614581754906 1103 6 0.10194951360063698 1103 7 0.05691209968799171 1103 8 0.1262088498497249 1103 9 -0.015044420282869453 1103 10-11 0.054464457095427576 1103 12-13 0.11055373711553784 1103 14-15 0.06133547074178125 1103 16-17 0.08312476437250638 1103 18-19 -0.04611956042025156 1103 20-21 0.01665517131677774 1103 22-23 0.0519254725842444 1103 24-25 0.1163972481446578 1103 26-27 0.003613726404310569 1103 28-29 0.06021456452798901 1103 30-31 0.1374113759219 1103 32-33 0.2837733168300858 1103 34-35 0.16504300029940566 1103 36-37 0.2131625942207691 1103 38-39 0.13088334113614764 1103 40-41 0.26213921858974487 1103 42-43 0.3217670162321724 1103 44-45 0.27436413467327725 1103 46-47 0.3745814935360414 1103 48-49 0.1951555502671667 1103 50-51 0.30286679996186194 1103 52-53 0.33867133318528175 1103 54-55 0.44520294607483635 1103 56-57 0.5575689099301613 1103 58-59 0.44009089972082904 1103 60-61 0.45455023108524273 1103 62-63 0.5567182707900216 1103 64-65 0.3585852014951101 1103 66-67 0.39305452855893463 1103 68-69 0.5797522831617314 1103 70-71 0.6096974292684934 1103 72-73 0.5297142554652652 1103 74-75 0.6124578724887115 1103 76-77 0.2991413793644675 1103 78-79 0.5706790709055731 1103 80-81 0.39358270289411124 1103 82-83 0.5638915764066574 1103 84-85 0.7536514378230663 1103 86-87 0.881924909900146 1103 88-89 0.7835497454922091 1103 90-91 0.6447117683507493 1103 92-93 0.8256127078625468 1103 94-95 0.9320969747187036 1103 96-97 0.6884166980463 1103 98-99 1.0566592065647669 1103 100-101 0.668366136289837 1104 1 0.061004860970790276 1104 2 -0.0279968497951657 1104 3 0.014329869639894355 1104 4 0.04973995207089388 1104 5 0.02249402086609109 1104 6 0.08484289928471611 1104 7 0.14122931071616307 1104 8 0.06571525706377201 1104 9 0.07414572511989803 1104 10-11 0.08990303844763048 1104 12-13 0.20407619472437943 1104 14-15 0.2004906677042868 1104 16-17 0.13799683582264777 1104 18-19 0.11896047582257552 1104 20-21 0.1356770464516046 1104 22-23 0.1961335236693742 1104 24-25 0.2197980334059082 1104 26-27 0.11010507558668792 1104 28-29 0.13035133805346533 1104 30-31 0.07612691287663864 1104 32-33 0.1263573440985013 1104 34-35 0.22950561409756887 1104 36-37 0.2704202203156072 1104 38-39 0.28152276815431065 1104 40-41 0.33957374419701836 1104 42-43 0.3263111763909521 1104 44-45 0.3952542412444444 1104 46-47 0.4288024884879391 1104 48-49 0.22579929190758463 1104 50-51 0.35621667268051027 1104 52-53 0.3712937605917688 1104 54-55 0.44818693882626803 1104 56-57 0.41876060859694064 1104 58-59 0.5853620737295486 1104 60-61 0.5470082713838096 1104 62-63 0.5411165967165985 1104 64-65 0.5084199514627485 1104 66-67 0.10904365571085606 1104 68-69 0.2103830809353866 1104 70-71 0.25663706770314576 1104 72-73 0.36016383910421723 1104 74-75 0.41494352636971144 1104 76-77 0.27470724207318753 1104 78-79 0.3981062616550233 1104 80-81 0.3621032189229325 1104 82-83 0.4723788698441176 1104 84-85 0.4560702150591176 1104 86-87 0.5177902073933502 1104 88-89 0.742668268165545 1104 90-91 0.9087886549193307 1104 92-93 0.8483136613940658 1104 94-95 1.041995149633486 1104 96-97 0.9833340730301181 1104 98-99 1.2864201764918484 1104 100-101 1.0611835846222348 1105 1 0.15433752573202497 1105 2 0.08702262236716507 1105 3 0.11012956543959262 1105 4 0.08823131913368343 1105 5 -0.02449489755139922 1105 6 0.0829006973425166 1105 7 0.02786956878499325 1105 8 0.03659225651220055 1105 9 0.14115503498635462 1105 10-11 0.16845775271662689 1105 12-13 0.2090829640168579 1105 14-15 0.15993174143107325 1105 16-17 0.18337198834066726 1105 18-19 0.2911085979707053 1105 20-21 0.23563275355017055 1105 22-23 0.10041489223645783 1105 24-25 0.12670104030891594 1105 26-27 0.1086943884617142 1105 28-29 0.056839328827173574 1105 30-31 0.16901414147815785 1105 32-33 0.30275089906348285 1105 34-35 0.14932961249299836 1105 36-37 0.15575425564964007 1105 38-39 0.16245258479841596 1105 40-41 0.19009857329328383 1105 42-43 0.19472532337652382 1105 44-45 0.17772260942710005 1105 46-47 0.1476319858888573 1105 48-49 0.2132301364812932 1105 50-51 0.16829944190613588 1105 52-53 0.24658250016621963 1105 54-55 0.33772127836060406 1105 56-57 0.18407425248202003 1105 58-59 0.26248771514090663 1105 60-61 0.3859141531468353 1105 62-63 0.3144806843663943 1105 64-65 0.11857472590323681 1105 66-67 0.08127367794087803 1105 68-69 0.15083336424280702 1105 70-71 0.3401876869680578 1105 72-73 0.26090428270180155 1105 74-75 0.2376181490443372 1105 76-77 0.3033756135986998 1105 78-79 0.2910478117394284 1105 80-81 -0.07029745633488815 1105 82-83 0.03392928853739363 1105 84-85 0.12595187779792738 1105 86-87 0.5735758631790056 1105 88-89 0.8795082621483985 1105 90-91 0.6759793149671331 1105 92-93 0.8602994876656069 1105 94-95 0.7415438634679141 1105 96-97 0.6706186674575711 1105 98-99 1.2521190850479016 1105 100-101 1.1009494172452108 1106 1 0.0789803341682429 1106 2 0.07641718166377132 1106 3 0.08036935108454202 1106 4 0.033701544479242784 1106 5 0.11454387407864175 1106 6 0.08943403388475701 1106 7 0.15335311109845406 1106 8 0.11784421194047212 1106 9 0.027120876161454532 1106 10-11 0.21363155519230048 1106 12-13 0.1538087227230278 1106 14-15 -0.0062078621083472285 1106 16-17 -0.03492199575919841 1106 18-19 0.0084667842959405 1106 20-21 0.04905882432263553 1106 22-23 0.075790535020829 1106 24-25 0.3135982670102422 1106 26-27 0.07597596211017077 1106 28-29 0.10404276556756287 1106 30-31 0.18056861670137891 1106 32-33 0.324636453362217 1106 34-35 0.16179381311391694 1106 36-37 0.1744978879729686 1106 38-39 0.3448003822842409 1106 40-41 0.4679190028056084 1106 42-43 0.5362618287153467 1106 44-45 0.6676077305232653 1106 46-47 0.6801947802058876 1106 48-49 0.8073935340730003 1106 50-51 0.5812983268106322 1106 52-53 0.5948348486857142 1106 54-55 0.6780327830977555 1106 56-57 0.8023012506288723 1106 58-59 0.6570430006133705 1106 60-61 0.6827036407644655 1106 62-63 0.7422946960628636 1106 64-65 0.4675342375074507 1106 66-67 0.40185280559925474 1106 68-69 0.5161903953611997 1106 70-71 0.5717765777288051 1106 72-73 0.887768056537972 1106 74-75 0.8103853507964018 1106 76-77 0.6268884853306282 1106 78-79 0.8582696194777135 1106 80-81 0.8905033266386475 1106 82-83 0.765217344179856 1106 84-85 0.9079872078742177 1106 86-87 1.271421482618571 1106 88-89 1.2758705638980565 1106 90-91 1.3123859798688997 1106 92-93 1.2366065159761988 1106 94-95 1.1600789167758983 1106 96-97 1.1182785713507783 1106 98-99 1.26094879632641 1106 100-101 1.2519060729213685 1107 1 -0.001693614961695289 1107 2 0.0953683914491279 1107 3 0.050462448717254915 1107 4 0.04236433032190945 1107 5 -0.05750529620596723 1107 6 0.08555997362142165 1107 7 0.11796992330252465 1107 8 0.07829820435311774 1107 9 0.10260105594157665 1107 10-11 0.04084604811105663 1107 12-13 0.04918529246275227 1107 14-15 0.09306610278629535 1107 16-17 0.14258668068418956 1107 18-19 -0.025928377655233703 1107 20-21 0.06373912460132658 1107 22-23 0.11374680434137474 1107 24-25 0.12143933688769692 1107 26-27 0.10488352238090926 1107 28-29 0.0522073085509831 1107 30-31 0.03406139732295799 1107 32-33 0.14154964522419533 1107 34-35 -0.016556168116714787 1107 36-37 0.008887274733581307 1107 38-39 0.08668058141905277 1107 40-41 -0.08585621585169179 1107 42-43 -0.06932036122130825 1107 44-45 -0.10422502620151874 1107 46-47 -0.002802278349086862 1107 48-49 -0.0824544613505438 1107 50-51 -0.1272422435128533 1107 52-53 -0.2435604912037661 1107 54-55 -0.24912801615739966 1107 56-57 -0.20095665923601302 1107 58-59 -0.18195014954235944 1107 60-61 -0.12762778493497962 1107 62-63 1.8075779652804158E-4 1107 64-65 -0.048530547643757416 1107 66-67 -0.1539234385445809 1107 68-69 -0.0341478044806891 1107 70-71 0.08500020325296731 1107 72-73 0.1812850929599108 1107 74-75 0.2213533741906062 1107 76-77 0.14271719005683892 1107 78-79 0.4692445887092127 1107 80-81 0.2655656635773127 1107 82-83 0.05528916725923594 1107 84-85 0.161682744510383 1107 86-87 0.29049756138904925 1107 88-89 0.6183042448094156 1107 90-91 0.7339733300163651 1107 92-93 0.5884311249996976 1107 94-95 0.6089989171192869 1107 96-97 0.7719052739589038 1107 98-99 0.9195086042779117 1107 100-101 0.9607049659578131 1108 1 0.016477959301028022 1108 2 0.07589925410093912 1108 3 0.013402225855109862 1108 4 0.05252288342524736 1108 5 0.07722500416850409 1108 6 0.14885032043499535 1108 7 0.1690586242597618 1108 8 0.13250560956841184 1108 9 0.19659470471173535 1108 10-11 0.1928714985589437 1108 12-13 0.1915530036297639 1108 14-15 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-0.6080069688430854 2316 58-59 -0.6987682034908431 2316 60-61 -0.5983244219738779 2316 62-63 -0.6370790295357978 2316 64-65 -0.6501892567599299 2316 66-67 -0.7220577854721242 2316 68-69 -0.5536354114268534 2316 70-71 -0.43889291327636926 2316 72-73 -0.24825598950517147 2316 74-75 -0.5040994395598339 2316 76-77 -0.5709253367215261 2316 78-79 -0.6195052171975881 2316 80-81 -0.818712364942531 2316 82-83 -0.7680311225779484 2316 84-85 -1.1702825701414916 2316 86-87 -1.4087089791132463 2316 88-89 -1.557997998745556 2316 90-91 -1.3105839718185415 2316 92-93 -1.2225152766910412 2316 94-95 -0.9007807814141771 2316 96-97 -0.5594620062480082 2316 98-99 -1.0676409641042888 2316 100-101 -0.6371007901155963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 12.0 5 30.0 6 46.0 7 116.0 8 343.0 9 674.0 10 1078.0 11 1661.0 12 2457.0 13 3259.0 14 4177.0 15 5546.0 16 7201.0 17 10047.0 18 10106.0 19 7440.0 20 7079.0 21 7625.0 22 8472.0 23 9224.0 24 10640.0 25 12242.0 26 14951.0 27 17877.0 28 21478.0 29 25959.0 30 30935.0 31 37607.0 32 46582.0 33 59468.0 34 79688.0 35 114073.0 36 175809.0 37 250942.0 38 156370.0 39 7804.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.16309965356505 19.821059575453358 15.285776071086879 22.730064699894715 2 15.422259471323848 20.967937052499476 39.95122804544042 23.658575430736253 3 19.217403337275822 24.718956398534058 30.435213977812413 25.62842628637771 4 13.224649505970735 17.506555580794434 37.00019407826315 32.268600834971686 5 13.706712061833509 37.438132114208535 34.44410502506046 14.411050798897495 6 31.463741741882874 36.61029693974262 17.263827205943148 14.662134112431366 7 27.69079068180651 33.19460653895795 21.038692939467598 18.07590983976794 8 25.426689329437842 35.718755357822005 19.72035323984505 19.1342020728951 9 25.74217529531662 17.39446015346978 20.836346768248795 36.027017782964805 10-11 24.77134882652275 26.71717923345178 27.31825615023572 21.193215789789743 12-13 25.839693095252393 24.076061273031563 27.816201792656532 22.268043839059512 14-15 22.725239358021813 25.587222402570557 26.463106097330662 25.22443214207697 16-17 21.848087847358514 28.585465707798047 27.212480841919838 22.353965602923605 18-19 21.5814286634326 27.58664355127596 29.316250920351116 21.51567686494032 20-21 22.741707940934063 26.606041142851172 29.00717219267533 21.64507872353943 22-23 22.251986691278923 26.452258052724886 28.982411983767047 22.31334327222914 24-25 22.241562374893387 26.550582235296165 29.169205061704755 22.038650328105692 26-27 22.20287723451289 26.54888513689927 29.301535221319035 21.946702407268802 28-29 21.94990343953679 26.8606927114474 29.314390250482802 21.87501359853301 30-31 22.183188993064533 26.683628932804538 28.964353131927094 22.16882894220384 32-33 22.116436514215145 26.729015396585442 29.004213151891918 22.150334937307502 34-35 22.468804509419577 26.669185052624982 28.87297586179284 21.989034576162606 36-37 22.532939980291726 26.511156416158 29.10831368889477 21.847589914655504 38-39 22.36104776461331 26.688421111269715 29.062942821033282 21.88758830308369 40-41 22.41053827173388 26.782206439764828 28.9058318210896 21.90142346741169 42-43 22.14322797559703 26.93278303785559 28.5829296706539 22.34105931589348 44-45 22.338090942440022 26.837562875244288 28.50753458546399 22.3168115968517 46-47 22.49936466934956 27.023136479467507 28.152545500118364 22.32495335106457 48-49 22.56890970338616 27.003038767678095 28.10344429934059 22.324607229595152 50-51 22.42582665405876 27.325112085182564 28.01413733247056 22.23492392828811 52-53 22.594060508902796 27.449106674290547 27.718814538637677 22.23801827816898 54-55 22.306424338632628 27.179964508916722 27.90645253655068 22.60715861589997 56-57 22.421609352657608 27.44684387839735 27.5674247903215 22.56412197862354 58-59 22.667474317065444 27.21646945375679 27.67268749151445 22.443368737663313 60-61 22.51368250836486 27.10975205906683 28.121454808449236 22.255110624119073 62-63 22.448625248910364 27.064389455948085 28.083964602509294 22.403020692632253 64-65 22.324967319806373 27.24562102811628 28.005925080112164 22.42348657196518 66-67 22.248303076351338 27.219708422433246 27.79725863823128 22.734729862984132 68-69 22.115969576785197 27.336332486503373 27.89390865550731 22.65378928120412 70-71 22.384316735725456 27.1378416930587 27.617336845888808 22.86050472532704 72-73 22.182927901230702 27.093369050696204 27.718596962109483 23.005106085963618 74-75 22.15512162092773 27.564857387288832 27.421822577655234 22.8581984141282 76-77 22.309899225890025 27.100121214655342 27.637638304702193 22.952341254752437 78-79 22.18825501486531 27.15416402665311 27.79451236918878 22.8630685892928 80-81 22.286484882295042 27.217264844645467 27.595544713437658 22.900705559621834 82-83 22.21547734984826 27.21199177386662 27.697448523569197 22.87508235271592 84-85 22.251275979215322 27.08435322464066 27.51794029605242 23.1464305000916 86-87 22.10538557328238 27.02351546870869 27.775292663900274 23.09580629410865 88-89 22.233848243705886 26.85842609019083 27.64532417587461 23.26240149022868 90-91 22.060503492434332 26.82553904584833 27.71851359338707 23.39544386833027 92-93 22.039414423235083 27.03649454622675 27.640748080322293 23.28334295021588 94-95 21.982939248214663 26.91635375276923 27.616129168419985 23.484577830596127 96-97 21.76596424260305 26.783278982716592 27.74400990060234 23.706746874078018 98-99 21.774536757813827 26.871266930717542 27.47321415361425 23.880982157854383 100-101 22.02782383171673 26.0029677593417 28.36281510685239 23.60639330208918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 256.5 2 157.0 3 175.5 4 165.0 5 158.5 6 282.0 7 612.5 8 1020.5 9 1191.5 10 1105.5 11 1021.5 12 1053.0 13 1137.0 14 1232.5 15 1363.5 16 1473.0 17 1551.0 18 1650.5 19 1805.5 20 2033.5 21 2371.0 22 2869.0 23 3546.0 24 4290.5 25 5197.0 26 6630.5 27 8311.0 28 9860.5 29 11615.5 30 13558.5 31 15698.5 32 17788.5 33 19558.5 34 21592.0 35 23966.5 36 26772.0 37 29171.0 38 30508.0 39 31317.0 40 32517.0 41 34103.5 42 35298.5 43 36813.0 44 38785.0 45 40360.5 46 41655.0 47 45053.5 48 52545.5 49 52132.5 50 44682.5 51 41703.0 52 40538.5 53 39932.5 54 39001.0 55 36414.0 56 32818.5 57 29121.5 58 26214.0 59 23251.5 60 19467.0 61 16025.5 62 12726.5 63 9688.5 64 7065.0 65 4917.0 66 3413.5 67 2529.5 68 1893.0 69 1368.5 70 971.5 71 657.0 72 456.0 73 290.5 74 164.5 75 88.0 76 53.5 77 22.0 78 12.0 79 7.5 80 3.0 81 2.5 82 1.0 83 2.5 84 2.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39215993441373137 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.703061127690442E-4 16-17 0.002436857115753324 18-19 2.610918338307133E-4 20-21 0.0 22-23 0.0 24-25 8.703061127690443E-5 26-27 8.703061127690443E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011923193744935907 36-37 0.023063111988379674 38-39 0.011923193744935907 40-41 0.005744020344275692 42-43 0.0054394132048065265 44-45 0.0016970969198996364 46-47 0.0011313979465997577 48-49 0.003089586700330107 50-51 5.656989732998788E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.832755014921398E-4 60-61 0.0015230356973458274 62-63 0.0011313979465997577 64-65 6.09214278938331E-4 66-67 0.002610918338307133 68-69 0.002610918338307133 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09203487142532643 78-79 0.0032201326172454636 80-81 3.4812244510761773E-4 82-83 0.0 84-85 1.3054591691535666E-4 86-87 0.0935143918170338 88-89 0.4382426430848523 90-91 0.38950550076978574 92-93 0.0 94-95 8.26790807130592E-4 96-97 0.0 98-99 0.0 100-101 5.221836676614266E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1149021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.42719915017327 #Duplication Level Percentage of deduplicated Percentage of total 1 80.50035165597312 52.669125394543336 2 11.798325604620809 15.438627979442282 3 3.5392908901789295 6.946976697663926 4 1.4871338763613042 3.891960171666408 5 0.7657846255998316 2.505157160263053 6 0.4863879602974675 1.9093801165577386 7 0.30822493475944235 1.411640593268864 8 0.19554781670641624 1.023531675762582 9 0.14992159011736958 0.882805476016781 >10 0.7218952658921104 8.156600014303587 >50 0.03183954111203302 1.41671924169295 >100 0.012769038142923073 1.630068497109348 >500 0.0015961264662247238 0.6978540374964125 >1k 9.310737719644223E-4 1.41955294421268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3895 0.33898423092354274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3511 0.3055644761932114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2377 0.20687176300520183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2237 0.1946874774264352 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1696 0.1476039167256299 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1446 0.1258462639064038 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1150 0.1000852029684401 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.703061127690443E-5 0.0 0.0 0.0 0.0 3 8.703061127690443E-5 0.0 0.0 0.0 0.0 4 8.703061127690443E-5 0.0 0.0 0.0 0.0 5 8.703061127690443E-5 0.0 0.0 0.0 0.0 6 8.703061127690443E-5 0.0 0.0 0.0 0.0 7 8.703061127690443E-5 0.0 0.0 0.0 0.0 8 8.703061127690443E-5 0.0 0.0 0.0 0.0 9 8.703061127690443E-5 0.0 0.0 0.0 0.0 10-11 8.703061127690443E-5 0.0 0.0 0.0 0.0 12-13 8.703061127690443E-5 0.0 0.0 0.0 0.0 14-15 2.6109183383071327E-4 0.0 0.0 0.0 0.0 16-17 2.6109183383071327E-4 0.0 0.0 0.0 0.0 18-19 2.6109183383071327E-4 0.0 0.0 4.3515305638452216E-5 0.0 20-21 2.6109183383071327E-4 0.0 0.0 1.7406122255380886E-4 0.0 22-23 2.6109183383071327E-4 0.0 0.0 3.9163775074606993E-4 4.3515305638452216E-5 24-25 4.3515305638452213E-4 0.0 0.0 6.092142789383309E-4 8.703061127690443E-5 26-27 4.3515305638452213E-4 0.0 0.0 9.573367240459487E-4 8.703061127690443E-5 28-29 5.221836676614265E-4 0.0 0.0 0.0031331020059685594 8.703061127690443E-5 30-31 6.092142789383309E-4 0.0 0.0 0.008311423376944373 8.703061127690443E-5 32-33 6.092142789383309E-4 0.0 0.0 0.01962540284294195 8.703061127690443E-5 34-35 6.527295845767831E-4 0.0 0.0 0.035334428178423194 8.703061127690443E-5 36-37 6.962448902152355E-4 0.0 0.0 0.06231391767426357 8.703061127690443E-5 38-39 6.962448902152355E-4 0.0 0.0 0.09995465705152473 8.703061127690443E-5 40-41 6.962448902152355E-4 0.0 0.0 0.1395100698768778 8.703061127690443E-5 42-43 7.397601958536877E-4 0.0 0.0 0.18372162040554524 8.703061127690443E-5 44-45 0.001044367335322853 0.0 0.0 0.24107479323702524 8.703061127690443E-5 46-47 0.0012184285578766619 0.0 0.0 0.3086105475879031 8.703061127690443E-5 48-49 0.0012184285578766619 0.0 0.0 0.3825430518676334 8.703061127690443E-5 50-51 0.0012184285578766619 0.0 0.0 0.46174090812961643 8.703061127690443E-5 52-53 0.0012184285578766619 0.0 0.0 0.5451597490385294 8.703061127690443E-5 54-55 0.0012184285578766619 0.0 0.0 0.6377603194371556 1.3054591691535666E-4 56-57 0.0012184285578766619 0.0 0.0 0.7515528436817082 1.7406122255380886E-4 58-59 0.0012184285578766619 0.0 0.0 0.8814460310124881 1.7406122255380886E-4 60-61 0.0012184285578766619 0.0 0.0 1.0233494426994807 1.7406122255380886E-4 62-63 0.0012184285578766619 0.0 0.0 1.1673851043627574 1.7406122255380886E-4 64-65 0.0012184285578766619 0.0 0.0 1.3251280873021467 1.7406122255380886E-4 66-67 0.0012184285578766619 0.0 0.0 1.5080229169005614 1.7406122255380886E-4 68-69 0.0012184285578766619 0.0 0.0 1.7177666900779012 1.7406122255380886E-4 70-71 0.0012184285578766619 0.0 0.0 1.9489199936293593 1.7406122255380886E-4 72-73 0.0012184285578766619 0.0 0.0 2.1879060521957387 1.7406122255380886E-4 74-75 0.0012184285578766619 0.0 0.0 2.4415132534566384 1.7406122255380886E-4 76-77 0.0012184285578766619 0.0 0.0 2.7147023422548413 1.7406122255380886E-4 78-79 0.0012184285578766619 0.0 0.0 3.0395440988458873 1.7406122255380886E-4 80-81 0.0013489744747920186 0.0 0.0 3.385925931727967 1.7406122255380886E-4 82-83 0.0014795203917073753 0.0 0.0 3.735092744170907 1.7406122255380886E-4 84-85 0.0015230356973458276 0.0 0.0 4.110238194080004 1.7406122255380886E-4 86-87 0.0015665510029842797 0.0 0.0 4.526201000677968 1.7406122255380886E-4 88-89 0.0015665510029842797 0.0 0.0 4.973146704890511 1.7406122255380886E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 380 0.0 23.835037 1 GTGTACG 120 1.6698489E-4 23.835037 1 GTACATG 3895 0.0 22.396982 1 TACATGG 3845 0.0 22.214325 2 AGTACTT 3930 0.0 20.466944 12-13 GAGTACT 3830 0.0 20.38182 12-13 ACATGGG 4060 0.0 20.219807 3 GCACCGT 165 6.3718086E-5 20.131262 6 CCGTTGC 120 0.0047912854 19.771776 9 GTATCAA 8485 0.0 19.495012 1 CATGGGG 2210 0.0 18.680304 4 GTACTTT 4095 0.0 18.599316 14-15 GCTATAT 360 9.094947E-11 18.538363 1 GGTATCA 6750 0.0 18.503052 1 TGCACCG 155 9.6897315E-4 18.368618 5 GTGTAGC 595 0.0 17.62591 1 GTATAGG 330 1.15778676E-8 17.334574 1 TATCAAC 9860 0.0 16.747856 2 CAACGCA 9860 0.0 16.69973 5 TCAACGC 9845 0.0 16.628777 4 >>END_MODULE