FastQCFastQC Report
Thu 26 May 2016
SRR938902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152837
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.3502663429435384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33170.2877249775987412No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21600.18736386843933706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.17565362666187848No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16120.13982896107602374No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14160.12282742486578761No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12230.10608611625060611No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG650.00594691829.1265965
CGAACGC350.00834623927.17415254-55
GTACATG42050.022.779291
TACATGG41150.022.4288942
GTGTACG1302.97554E-421.885811
GAGTACT37000.021.10694312-13
ACATGGG42150.020.9984463
GTATCAA91350.020.6599521
TTTGCGT2309.367068E-820.5785753
ACGGTCC1150.003802045720.577688
AGTACTT38800.020.31073412-13
GTGTAGC7150.019.8961931
GTACTTT39800.019.3247314-15
GTGTAGG4101.8189894E-1218.5050771
TATCAAC104600.018.09972
CATGGGG23050.018.0698644
ATCAACG104150.018.041573
CAACGCA104250.017.9334625
TCAACGC105100.017.8334584
AACGCAG105250.017.7615326
Produced by FastQC (version 0.11.5)