##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1152837 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.721851397899268 33.0 31.0 34.0 30.0 34.0 2 31.790804771186213 33.0 31.0 34.0 30.0 34.0 3 31.833829066901913 34.0 31.0 34.0 30.0 34.0 4 35.3712589030366 37.0 35.0 37.0 33.0 37.0 5 35.406547499776636 37.0 35.0 37.0 33.0 37.0 6 35.48436769465241 37.0 35.0 37.0 33.0 37.0 7 35.3865750318562 37.0 35.0 37.0 33.0 37.0 8 35.37385944413651 37.0 35.0 37.0 33.0 37.0 9 36.97365716055262 39.0 37.0 39.0 33.0 39.0 10-11 36.85620343552471 39.0 37.0 39.0 33.0 39.0 12-13 36.72951857027489 39.0 37.0 39.0 32.0 39.0 14-15 37.92580477552334 40.0 38.0 41.0 33.0 41.0 16-17 37.850055124878885 40.0 38.0 41.0 32.0 41.0 18-19 37.78932190760706 40.0 38.0 41.0 32.0 41.0 20-21 37.667016239069355 40.0 37.5 41.0 32.0 41.0 22-23 37.75051763605783 40.0 38.0 41.0 32.0 41.0 24-25 37.682237818529416 40.0 38.0 41.0 32.0 41.0 26-27 37.59487637888097 40.0 37.0 41.0 32.0 41.0 28-29 37.464918717910685 40.0 37.0 41.0 31.5 41.0 30-31 37.27985092428504 40.0 37.0 41.0 31.0 41.0 32-33 37.087588705081465 40.0 36.5 41.0 30.5 41.0 34-35 36.7756859816262 40.0 36.0 41.0 30.0 41.0 36-37 36.503083263288744 39.0 36.0 41.0 30.0 41.0 38-39 36.292764284976975 39.0 35.0 41.0 30.0 41.0 40-41 36.13754503021676 39.0 35.0 41.0 29.5 41.0 42-43 35.93781601388575 39.0 35.0 41.0 28.5 41.0 44-45 35.850080713925735 39.0 35.0 41.0 27.5 41.0 46-47 35.62483638189961 39.0 35.0 41.0 26.5 41.0 48-49 35.429435384187016 39.0 35.0 41.0 26.0 41.0 50-51 34.278990438370734 38.0 34.0 40.0 24.0 40.5 52-53 34.414871746829775 38.0 33.5 39.5 24.0 40.5 54-55 34.907949692801324 39.0 35.0 40.0 24.0 41.0 56-57 34.80024626204745 39.0 34.5 40.5 24.0 41.0 58-59 34.59423795384777 38.0 34.0 40.0 23.5 41.0 60-61 34.32280018771084 38.0 34.0 40.0 23.0 41.0 62-63 33.96073816159613 37.5 34.0 40.0 22.5 41.0 64-65 33.54454662714677 37.0 33.0 40.0 21.5 41.0 66-67 33.15151751722056 36.5 33.0 39.0 20.5 41.0 68-69 32.75859510060833 36.0 32.5 39.0 19.5 41.0 70-71 32.330126028224285 35.0 32.0 39.0 18.0 40.0 72-73 31.892433188733534 35.0 31.5 38.0 15.5 40.0 74-75 31.425728008382798 35.0 31.0 37.0 12.0 39.0 76-77 31.008782681333095 35.0 31.0 37.0 8.0 39.0 78-79 30.57365568592958 35.0 30.5 36.0 7.0 39.0 80-81 30.138102784695498 34.0 30.0 36.0 3.5 37.5 82-83 29.748165178598533 34.0 30.0 35.0 2.0 37.0 84-85 29.385941811374895 34.0 29.5 35.0 2.0 37.0 86-87 28.984465713713213 34.0 29.0 35.0 2.0 36.0 88-89 28.55492450363755 34.0 29.0 35.0 2.0 36.0 90-91 28.120077686611378 34.0 28.5 35.0 2.0 35.5 92-93 27.699869972945002 34.0 27.0 35.0 2.0 35.0 94-95 27.217845194073405 34.0 26.5 35.0 2.0 35.0 96-97 26.6198729742366 33.0 25.0 35.0 2.0 35.0 98-99 25.887694010514927 33.0 24.0 35.0 2.0 35.0 100-101 23.220409737022663 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12967599627571857 1101 2 0.13586596459899525 1101 3 0.19693117662178494 1101 4 0.026216124378649397 1101 5 -0.08526792155547014 1101 6 0.06487843997191334 1101 7 -0.023094723255972838 1101 8 -0.06695858969603563 1101 9 0.074646573103621 1101 10-11 0.13348697155672085 1101 12-13 0.3796247844246068 1101 14-15 0.22484203645055345 1101 16-17 0.3122901578530488 1101 18-19 0.33378597788244946 1101 20-21 0.44383456643194563 1101 22-23 0.3352325367321498 1101 24-25 0.48680107760759483 1101 26-27 0.1578057765187424 1101 28-29 0.2050311006312313 1101 30-31 0.29131636488651935 1101 32-33 0.15179811142413513 1101 34-35 -0.023162002367577372 1101 36-37 0.17691025490769619 1101 38-39 0.2978500454957427 1101 40-41 0.4240918456408096 1101 42-43 0.1991357034231882 1101 44-45 0.34154471626367666 1101 46-47 0.07677898945146211 1101 48-49 -0.06331166460773119 1101 50-51 0.09260700588927051 1101 52-53 -0.11645316089132507 1101 54-55 0.03758641263223694 1101 56-57 0.08391705405587402 1101 58-59 -0.02232446535840893 1101 60-61 -0.12771803059362696 1101 62-63 0.03866160833919707 1101 64-65 -0.01739996250494613 1101 66-67 -0.07210317861751747 1101 68-69 -0.2894938008528456 1101 70-71 -0.2783297946762957 1101 72-73 -0.3096552042099354 1101 74-75 -0.19857223993700757 1101 76-77 -0.15187923552383253 1101 78-79 -0.21017975131558586 1101 80-81 -0.38188192638247287 1101 82-83 -0.304094024938113 1101 84-85 -0.4139270470920202 1101 86-87 -0.37984381261137656 1101 88-89 -0.485987112046832 1101 90-91 -0.40927758303842054 1101 92-93 -0.2705292370811705 1101 94-95 -0.35878923512612104 1101 96-97 -0.24387436826107844 1101 98-99 -0.2977529654858735 1101 100-101 0.26442862839271797 1102 1 0.12104534727809835 1102 2 -0.009011711241981857 1102 3 0.04735591328505251 1102 4 0.0541468073552025 1102 5 0.07624244201910813 1102 6 0.05627327517408531 1102 7 0.14249311228599737 1102 8 -0.03251244630494199 1102 9 0.19657997439482244 1102 10-11 0.14051636333381623 1102 12-13 0.26884921527747707 1102 14-15 0.46032453730002487 1102 16-17 0.32562264171306055 1102 18-19 0.34539827825621217 1102 20-21 0.39097184132151597 1102 22-23 0.41358370900534425 1102 24-25 0.363198461229743 1102 26-27 0.4811960245649516 1102 28-29 0.31975247338012736 1102 30-31 0.2880416452127079 1102 32-33 0.3491689914784999 1102 34-35 0.12056463711594034 1102 36-37 0.13667495079624103 1102 38-39 0.2860805925565657 1102 40-41 0.31746170632718673 1102 42-43 0.3024698862298507 1102 44-45 0.35151753311723155 1102 46-47 0.28129394018201737 1102 48-49 0.2790600649199675 1102 50-51 0.2821967102725509 1102 52-53 0.05895033893877866 1102 54-55 0.3068312308449421 1102 56-57 0.35306843020266854 1102 58-59 0.26905762774386943 1102 60-61 0.17155566971218406 1102 62-63 0.3135341873402169 1102 64-65 0.11041604157252038 1102 66-67 0.2187267568225124 1102 68-69 0.0873028352327836 1102 70-71 -0.2241843648427917 1102 72-73 -0.14052506489631256 1102 74-75 -0.4401412171711243 1102 76-77 -0.4209881380042937 1102 78-79 -0.4335275256954674 1102 80-81 -0.49504451557717033 1102 82-83 -0.4543828458691337 1102 84-85 -0.46305208956568933 1102 86-87 -0.40097870897563226 1102 88-89 -0.42996859352831507 1102 90-91 -0.15162807573294756 1102 92-93 -0.1803449013693097 1102 94-95 -0.28347917736193295 1102 96-97 -0.5169333217099386 1102 98-99 -0.6983645862809773 1102 100-101 -0.3482497610058566 1103 1 -0.09115508845174958 1103 2 0.18357407307174256 1103 3 0.17128020522405407 1103 4 0.27146706879311466 1103 5 0.1267598712130109 1103 6 0.07328035578410663 1103 7 0.2616175676236878 1103 8 0.033813806418372394 1103 9 0.17311864541878208 1103 10-11 0.18543398315951976 1103 12-13 0.3091031640792181 1103 14-15 0.22701503294273095 1103 16-17 0.3245877833252635 1103 18-19 0.40530091986841654 1103 20-21 0.470747848946786 1103 22-23 0.2885632841687382 1103 24-25 0.42283217582669863 1103 26-27 0.23414060517497148 1103 28-29 0.2949941672799099 1103 30-31 0.23542726612775056 1103 32-33 0.38482394790551666 1103 34-35 0.22825799223576837 1103 36-37 0.2595473636502774 1103 38-39 0.2801641982210512 1103 40-41 0.10531233268883256 1103 42-43 0.21551454853921825 1103 44-45 0.16595108431548766 1103 46-47 0.033557692905326064 1103 48-49 -0.10113124771620363 1103 50-51 -0.18004797926993632 1103 52-53 -0.11444290724858774 1103 54-55 0.014568839777403753 1103 56-57 0.0355132831438425 1103 58-59 0.15252712665454737 1103 60-61 0.1079833657906164 1103 62-63 -0.02515045318266118 1103 64-65 -0.15654678522486165 1103 66-67 -0.039732529669862515 1103 68-69 0.08044009013473641 1103 70-71 -0.28734136375346964 1103 72-73 -0.21477275008149732 1103 74-75 -0.15121828688790018 1103 76-77 -0.23021335586921765 1103 78-79 0.0749828228884084 1103 80-81 -0.2448811168843612 1103 82-83 -0.040824158505298414 1103 84-85 -0.08351437170076537 1103 86-87 -0.0032492699778110534 1103 88-89 -0.0703621120468334 1103 90-91 -0.2526118720292452 1103 92-93 -0.2890220527854339 1103 94-95 -0.018929885422934944 1103 96-97 -0.13098901343107272 1103 98-99 -0.3351905666731376 1103 100-101 -0.4253126695025884 1104 1 0.15675915803679175 1104 2 0.15752030551445273 1104 3 0.20222658287744721 1104 4 0.29103090869173087 1104 5 0.22870437432843715 1104 6 0.18454144460024224 1104 7 0.33489121056380355 1104 8 0.2780284806183957 1104 9 0.3164316932591049 1104 10-11 0.1965747781220344 1104 12-13 0.2716627334635291 1104 14-15 0.24291147792107637 1104 16-17 0.3158289386742936 1104 18-19 0.3138586147147535 1104 20-21 0.24914891426519858 1104 22-23 0.27357523104803505 1104 24-25 0.5251116535962552 1104 26-27 0.5068834040449701 1104 28-29 0.14842625488155647 1104 30-31 0.4041422835530284 1104 32-33 0.3395132630391089 1104 34-35 0.42428912012677955 1104 36-37 0.33946852084744705 1104 38-39 0.4252346254044923 1104 40-41 0.4047788484243924 1104 42-43 0.45331685078866 1104 44-45 0.5067605678934797 1104 46-47 0.5694156570514863 1104 48-49 0.5163763758448923 1104 50-51 0.43397458779100617 1104 52-53 0.48476239426160816 1104 54-55 0.4707259379398323 1104 56-57 0.5336115181739416 1104 58-59 0.6066141474592825 1104 60-61 0.6430921747989515 1104 62-63 0.6949724478628596 1104 64-65 0.7803566958713475 1104 66-67 0.8039011367679834 1104 68-69 0.6635072038743104 1104 70-71 0.688146907183274 1104 72-73 0.8503220658969575 1104 74-75 0.546263297756731 1104 76-77 0.5512000061542288 1104 78-79 0.5146114795575372 1104 80-81 0.5815987120310488 1104 82-83 0.5825743986285055 1104 84-85 0.6916673066707624 1104 86-87 0.7805526428429737 1104 88-89 0.7991116312206721 1104 90-91 0.610984963697863 1104 92-93 0.7572465275820832 1104 94-95 0.7451572013866539 1104 96-97 0.4869773438928249 1104 98-99 0.44397532993685473 1104 100-101 0.757931667280598 1105 1 -0.06741745180417169 1105 2 0.009203627991055896 1105 3 0.17937820105955282 1105 4 0.10173776114084632 1105 5 0.10347121946912807 1105 6 0.09866101689811302 1105 7 0.0447464718402415 1105 8 0.18896411875569896 1105 9 0.16955604781337286 1105 10-11 0.07696752688675446 1105 12-13 0.08586412842070956 1105 14-15 0.1635284048844241 1105 16-17 0.18167393637055085 1105 18-19 0.14735665255972918 1105 20-21 0.21761064827006038 1105 22-23 0.26091655069658515 1105 24-25 0.22260931013174456 1105 26-27 0.07103951850136525 1105 28-29 0.08016709153810808 1105 30-31 0.02374715160406282 1105 32-33 0.34857492395966005 1105 34-35 0.3109981015538281 1105 36-37 0.36435849717448576 1105 38-39 0.19771202487384443 1105 40-41 0.34014689151236865 1105 42-43 0.2894864381800275 1105 44-45 0.19279908015099068 1105 46-47 0.38695192117184973 1105 48-49 0.3100990685253393 1105 50-51 0.1062994634682255 1105 52-53 0.2528007158643746 1105 54-55 0.33259737309339243 1105 56-57 0.2642409965743511 1105 58-59 0.299117444197492 1105 60-61 0.16842145142310017 1105 62-63 0.19224608507866492 1105 64-65 0.1289140750041895 1105 66-67 0.14436998854981908 1105 68-69 0.14523056384634714 1105 70-71 0.049627500119513 1105 72-73 0.2026083339892999 1105 74-75 0.3209657969226143 1105 76-77 0.12736895802979475 1105 78-79 0.2795214746322898 1105 80-81 0.1334830163795644 1105 82-83 0.2222313789751773 1105 84-85 0.19157754006394256 1105 86-87 0.06953915402010935 1105 88-89 0.20870315343989176 1105 90-91 0.18891191115659467 1105 92-93 0.13735011665756502 1105 94-95 -0.030328571003362725 1105 96-97 -0.04731270551852873 1105 98-99 -0.07594570722493188 1105 100-101 0.2558185772438968 1106 1 0.2635404739837526 1106 2 0.2458128501615242 1106 3 0.26334031874314334 1106 4 0.27636902957742393 1106 5 0.21810404045965726 1106 6 0.2810783434002033 1106 7 0.2223664066329718 1106 8 0.23303336266192076 1106 9 0.20057607575934355 1106 10-11 0.23045301050731126 1106 12-13 0.3249651996829357 1106 14-15 0.1865915938302365 1106 16-17 0.2767678658846009 1106 18-19 0.28448209891898557 1106 20-21 0.4196755450252212 1106 22-23 0.30093751017493275 1106 24-25 0.3727257516780895 1106 26-27 0.5970098647208957 1106 28-29 0.466438633224179 1106 30-31 0.3906001247448714 1106 32-33 0.5186378023946787 1106 34-35 0.5412030386753983 1106 36-37 0.406533625260181 1106 38-39 0.19614394538307778 1106 40-41 0.29233987396850836 1106 42-43 0.43597378486532534 1106 44-45 0.4359717241503631 1106 46-47 0.3229362403769471 1106 48-49 0.16975207271723747 1106 50-51 0.13007682723161196 1106 52-53 0.12067061184326633 1106 54-55 0.04072499302817789 1106 56-57 0.20767369336056163 1106 58-59 0.24832176029747188 1106 60-61 0.25203812585253615 1106 62-63 0.4304932516677056 1106 64-65 0.20961194800527494 1106 66-67 0.19721116228060254 1106 68-69 0.18182037909242865 1106 70-71 0.152911151726407 1106 72-73 0.09885115342727246 1106 74-75 0.2785965995930084 1106 76-77 0.41889977622572516 1106 78-79 0.2719061975014121 1106 80-81 0.20458998734680378 1106 82-83 0.199905653156204 1106 84-85 0.21613894357271235 1106 86-87 0.2215846438508784 1106 88-89 0.3534865721636926 1106 90-91 0.35185632972514114 1106 92-93 0.39307127796791974 1106 94-95 0.4625490877452805 1106 96-97 0.35263295509318127 1106 98-99 0.38409155406989726 1106 100-101 0.8929052097543781 1107 1 0.20136577278240964 1107 2 0.17039576102888887 1107 3 0.13617345236528777 1107 4 0.2883363383923694 1107 5 0.1705620242984125 1107 6 0.16498930293564484 1107 7 0.17310380178459894 1107 8 0.2126827927169188 1107 9 0.16871138776966887 1107 10-11 0.1829816613814259 1107 12-13 0.18165245196425417 1107 14-15 0.30105068376172284 1107 16-17 0.30583198853799587 1107 18-19 0.27641760756801403 1107 20-21 0.33102989412037687 1107 22-23 0.1484372797389497 1107 24-25 0.43080483730810215 1107 26-27 0.3882264130970867 1107 28-29 0.44857293131617126 1107 30-31 0.3955114837026912 1107 32-33 0.47199525469443415 1107 34-35 0.22012275477826648 1107 36-37 0.18379420227189058 1107 38-39 0.19390718008980912 1107 40-41 0.25914864282585626 1107 42-43 0.2452611231760713 1107 44-45 0.3714857500206463 1107 46-47 0.3628070426014247 1107 48-49 0.2999460998165091 1107 50-51 0.21026394125134118 1107 52-53 0.19145747391964107 1107 54-55 -0.12868232561869775 1107 56-57 -8.063508831455124E-4 1107 58-59 0.096588113571201 1107 60-61 0.028130392420877115 1107 62-63 0.1110045123061667 1107 64-65 0.0633227354818473 1107 66-67 0.1685264908823214 1107 68-69 0.21471818841433077 1107 70-71 0.21678339801638913 1107 72-73 0.08917591454197193 1107 74-75 0.2838720474069376 1107 76-77 0.267486435518439 1107 78-79 0.16094205334065848 1107 80-81 0.25298768913191694 1107 82-83 0.2069585305335373 1107 84-85 0.2649575750902109 1107 86-87 0.4725148371098591 1107 88-89 0.39693758147330627 1107 90-91 0.4538926157115597 1107 92-93 0.5684591785424757 1107 94-95 0.41490491163073884 1107 96-97 0.4506957565802594 1107 98-99 0.8423826408060044 1107 100-101 1.2174950337012866 1108 1 0.05059475227876575 1108 2 0.018395027626851856 1108 3 -0.013200310809075688 1108 4 -0.029286806764332596 1108 5 0.13298465354471745 1108 6 0.06463335005040705 1108 7 0.1159822151072234 1108 8 0.06592651612319145 1108 9 0.10692226614505529 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-2.4306326163425744 2316 88-89 -2.556853827268977 2316 90-91 -2.78036246379909 2316 92-93 -3.0840718712379456 2316 94-95 -3.178182247146939 2316 96-97 -3.3836603455290977 2316 98-99 -3.811523119905644 2316 100-101 -4.359895993910282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4028.0 3 1075.0 4 1117.0 5 1189.0 6 1512.0 7 1828.0 8 2174.0 9 2656.0 10 3332.0 11 4168.0 12 5163.0 13 7447.0 14 7166.0 15 6592.0 16 8125.0 17 10693.0 18 9339.0 19 7068.0 20 7119.0 21 7557.0 22 8223.0 23 9146.0 24 10412.0 25 11991.0 26 13847.0 27 15976.0 28 18910.0 29 22368.0 30 26831.0 31 32760.0 32 40206.0 33 51620.0 34 68801.0 35 98216.0 36 150090.0 37 230244.0 38 209759.0 39 34081.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.203861113358435 19.111571595971512 14.756770910811795 22.927796379858254 2 15.033415693308353 20.368032754151134 40.80499785563101 23.793553696909502 3 19.280677475199816 24.479730093435872 30.20586800207674 26.03372442928757 4 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22.346285996647023 24-25 22.242931967184994 26.529936028022426 29.15577921308084 22.07135279171174 26-27 22.22365052515466 26.460455430796188 29.260614377750898 22.05527966629825 28-29 21.99185457918293 26.771155709198315 29.28586657889286 21.951123132725893 30-31 22.217986282502636 26.658761187367674 28.98107895463569 22.142173575494 32-33 22.049981503769583 26.72344922567659 28.954155399596733 22.2724138709571 34-35 22.476909044601516 26.67374869116786 28.773235171829796 22.07610709240083 36-37 22.509759064107058 26.410854417623376 29.07197713316476 22.007409385104797 38-39 22.378966303269703 26.622702525401003 29.022657860462196 21.9756733108671 40-41 22.36627478304548 26.714739739597892 28.89839033108592 22.02059514627071 42-43 22.11085499702331 26.811932057318188 28.66127445956172 22.415938486096785 44-45 22.296561008359078 26.85562202762396 28.461610272043025 22.386206691973943 46-47 22.47896942093174 26.9285191488842 28.142591818771063 22.449919611412998 48-49 22.521274205475777 26.90368418411977 28.195602712802042 22.379438897602412 50-51 22.442735903299518 27.206695185654073 28.153920517355257 22.196648393691156 52-53 22.52144877031122 27.365472387734783 27.8305702018056 22.2825086401484 54-55 22.318358344962 27.085275382371915 27.974971940708414 22.621394331957667 56-57 22.44351421373434 27.428296815793825 27.634916765477634 22.493272204994202 58-59 22.598123309666114 27.07359978649791 27.87113648871047 22.457140415125508 60-61 22.4214601668665 26.978537828259913 28.23646525096441 22.363536753909177 62-63 22.35942216096446 26.934024809393375 28.263080761379694 22.44347226826247 64-65 22.346465240557713 27.112548234583695 28.112668657424937 22.428317867433652 66-67 22.237917258503355 27.110156899452125 27.91719304676437 22.734732795280145 68-69 22.133854896924422 27.25152308176356 28.005983667972757 22.60863835333926 70-71 22.472146925296336 26.960755222833328 27.75646385078467 22.810634001085674 72-73 22.2420847283023 26.92272282815713 27.850656255358626 22.984536188181952 74-75 22.17771298947733 27.57539675440866 27.554758936764067 22.69213131934994 76-77 22.446845545181617 27.062551640679462 27.629410343055522 22.8611924710834 78-79 22.28820552363972 27.060984736806297 27.837795638240152 22.81301410131383 80-81 22.39320525960008 27.185634226651256 27.703482413484814 22.717678100263853 82-83 22.40681872506137 27.123152992984473 27.77188449238167 22.69814378957249 84-85 22.42565314821124 27.00513916219573 27.660741136897315 22.908466552695717 86-87 22.330235564756098 27.054747018474078 27.780598425031993 22.834418991737827 88-89 22.4918947026778 26.91146293200624 27.630251866225073 22.96639049909088 90-91 22.34025519030198 26.85881200602963 27.796982131443293 23.003950672225095 92-93 22.400465308266725 27.04124331206634 27.667123073687485 22.891168305979456 94-95 22.34767697570511 26.960782602624967 27.627015847528625 23.064524574141302 96-97 22.12976634274385 26.78702947418391 27.835284549440114 23.247919633632126 98-99 22.283178303300353 26.84484866572942 27.52239056049051 23.349582470479717 100-101 22.550843243148922 26.342034776691065 27.95353219853948 23.15358978162053 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1317.0 1 765.0 2 219.0 3 228.0 4 214.5 5 218.5 6 351.0 7 735.0 8 1221.5 9 1462.0 10 1359.5 11 1249.0 12 1382.0 13 1549.0 14 1603.5 15 1687.5 16 1837.5 17 2016.5 18 2130.0 19 2184.5 20 2279.5 21 2503.5 22 2974.5 23 3604.5 24 4305.0 25 5204.5 26 6692.5 27 8376.5 28 9890.0 29 11556.5 30 13297.0 31 15540.5 32 17696.0 33 19458.0 34 21628.0 35 23877.5 36 26411.5 37 28923.0 38 30486.0 39 31258.0 40 32467.0 41 33932.0 42 35208.5 43 36770.0 44 38625.5 45 40397.5 46 41773.5 47 44765.0 48 52405.0 49 52072.5 50 44193.5 51 41394.0 52 40478.0 53 39765.5 54 38479.5 55 35821.5 56 32363.0 57 29046.0 58 26599.5 59 23515.5 60 19674.0 61 16252.0 62 12979.5 63 9858.5 64 6968.5 65 4954.0 66 3610.5 67 2723.0 68 2052.5 69 1493.0 70 1071.0 71 717.0 72 481.0 73 328.5 74 192.5 75 104.0 76 76.0 77 47.0 78 33.0 79 21.5 80 15.5 81 13.0 82 10.0 83 9.0 84 7.5 85 6.0 86 6.5 87 6.0 88 3.0 89 2.0 90 3.5 91 3.5 92 2.0 93 2.0 94 0.5 95 0.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28833217532053534 2 0.08526790864623533 3 0.08943155016710949 4 0.10565240359218173 5 0.10704028409913977 6 0.08188494991052508 7 0.09272776637113486 8 0.08804366966015144 9 0.0937686767513534 10-11 0.08635219029229631 12-13 0.09073268814238265 14-15 0.09953705510839782 16-17 0.08852075358441827 18-19 0.09901659991828854 20-21 0.10109842067872561 22-23 0.11480374068493639 24-25 0.1278151204376681 26-27 0.13093785157832374 28-29 0.12078897537119299 30-31 0.11406642916561491 32-33 0.10851490713778271 34-35 0.38075634283077314 36-37 0.5831700405174366 38-39 0.5659082767121457 40-41 0.5300402398604487 42-43 0.5577111074679248 44-45 0.5775317759579195 46-47 0.5664287319022551 48-49 0.588938418874481 50-51 0.5811749622886844 52-53 0.5841242083659702 54-55 0.5728043079810936 56-57 0.5453936679686721 58-59 0.5416203678403799 60-61 0.4885773097150768 62-63 0.563566228356654 64-65 0.5963549053335381 66-67 0.6106240517957006 68-69 0.611664962175919 70-71 0.6074579493892025 72-73 0.5988270674865571 74-75 0.5970054743211747 76-77 0.5808279921619449 78-79 0.5568003108852335 80-81 0.5518559865791954 82-83 0.5658215341804609 84-85 0.5758402965900643 86-87 0.5662552468388853 88-89 0.5788329139331926 90-91 0.592408120141876 92-93 0.6011257445762064 94-95 0.599998091664303 96-97 0.5972223306503868 98-99 0.6011257445762064 100-101 0.5783124587430833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1152837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.75294843507 #Duplication Level Percentage of deduplicated Percentage of total 1 81.45602820845853 54.374300507248385 2 11.29610004411018 15.080959675237574 3 3.3347737316029304 6.67817936844949 4 1.3917663385050834 3.71618026511584 5 0.7445960346069073 2.4851990351536237 6 0.42503679689820834 1.702347563181205 7 0.27774803860995934 1.297835034949071 8 0.2001230828437279 1.0687044663787686 9 0.14094701099461826 0.8467765701300897 >10 0.6894702487271819 7.869621104701769 >50 0.02882545202687453 1.3086433130140709 >100 0.012371221569984546 1.611902877272909 >500 0.0013022300269651412 0.5866750057302356 >1k 9.115610188755989E-4 1.3726752134369757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.3502663429435384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3317 0.2877249775987412 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2160 0.18736386843933706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 0.17565362666187848 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1612 0.13982896107602374 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1416 0.12282742486578761 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1223 0.10608611625060611 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.674253168487826E-5 0.0 10-11 0.0 0.0 0.0 8.674253168487826E-5 0.0 12-13 0.0 0.0 0.0 1.3011379752731739E-4 0.0 14-15 0.0 0.0 0.0 1.7348506336975651E-4 0.0 16-17 0.0 0.0 0.0 3.035988608970739E-4 0.0 18-19 0.0 0.0 0.0 4.337126584243913E-4 8.674253168487826E-5 20-21 8.674253168487826E-5 0.0 0.0 5.204551901092695E-4 8.674253168487826E-5 22-23 8.674253168487826E-5 0.0 0.0 8.240540510063435E-4 8.674253168487826E-5 24-25 8.674253168487826E-5 0.0 0.0 0.0011710241777458566 8.674253168487826E-5 26-27 8.674253168487826E-5 0.0 0.0 0.0015613655703278087 8.674253168487826E-5 28-29 1.3011379752731739E-4 0.0 0.0 0.003339587469867813 8.674253168487826E-5 30-31 1.7348506336975651E-4 0.0 0.0 0.009715163548706366 8.674253168487826E-5 32-33 1.7348506336975651E-4 0.0 0.0 0.021859117984589324 8.674253168487826E-5 34-35 1.7348506336975651E-4 0.0 0.0 0.039901564575044 8.674253168487826E-5 36-37 3.4697012673951303E-4 0.0 0.0 0.06791940230925968 8.674253168487826E-5 38-39 3.4697012673951303E-4 0.0 0.0 0.10699691283329733 8.674253168487826E-5 40-41 3.4697012673951303E-4 0.0 0.0 0.14924052576383307 8.674253168487826E-5 42-43 5.204551901092695E-4 0.0 0.0 0.1933057318597512 8.674253168487826E-5 44-45 5.204551901092695E-4 0.0 0.0 0.25038231770840114 8.674253168487826E-5 46-47 5.638264559517087E-4 0.0 0.0 0.32302918799448666 8.674253168487826E-5 48-49 6.071977217941479E-4 0.0 0.0 0.40053364005492537 8.674253168487826E-5 50-51 6.071977217941479E-4 0.0 0.0 0.4802933979391709 8.674253168487826E-5 52-53 6.505689876365869E-4 0.0 0.0 0.564346911141818 1.3011379752731739E-4 54-55 6.939402534790261E-4 0.0 0.0 0.6589396419441778 1.7348506336975651E-4 56-57 8.674253168487826E-4 0.0 0.0 0.7757818321237087 1.7348506336975651E-4 58-59 8.674253168487826E-4 0.0 0.0 0.9058956296510261 1.7348506336975651E-4 60-61 8.674253168487826E-4 0.0 0.0 1.048890693133548 1.7348506336975651E-4 62-63 8.674253168487826E-4 0.0 0.0 1.2004298959870303 1.7348506336975651E-4 64-65 8.674253168487826E-4 0.0 0.0 1.3622480888451707 1.7348506336975651E-4 66-67 8.674253168487826E-4 0.0 0.0 1.5480939629800223 1.7348506336975651E-4 68-69 9.541678485336609E-4 0.0 0.0 1.7601794529495498 2.1685632921219564E-4 70-71 9.975391143761E-4 0.0 0.0 1.9863606043178699 2.6022759505463477E-4 72-73 0.0010842816460609782 0.0 0.0 2.228415638984522 2.6022759505463477E-4 74-75 0.0011276529119034174 0.0 0.0 2.484392849986598 2.6022759505463477E-4 76-77 0.0011276529119034174 0.0 0.0 2.765785622772343 2.6022759505463477E-4 78-79 0.0011276529119034174 0.0 0.0 3.077625024179481 2.6022759505463477E-4 80-81 0.0012577667094307349 0.0 0.0 3.4281082234522313 2.6022759505463477E-4 82-83 0.001301137975273174 0.0 0.0 3.7846633999429233 2.6022759505463477E-4 84-85 0.001301137975273174 0.0 0.0 4.162123526569671 2.6022759505463477E-4 86-87 0.001344509241115613 0.0 0.0 4.571201305995557 2.6022759505463477E-4 88-89 0.0013878805069580521 0.0 0.0 5.010118516321041 2.6022759505463477E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 65 0.005946918 29.126596 5 CGAACGC 35 0.008346239 27.174152 54-55 GTACATG 4205 0.0 22.77929 1 TACATGG 4115 0.0 22.428894 2 GTGTACG 130 2.97554E-4 21.88581 1 GAGTACT 3700 0.0 21.106943 12-13 ACATGGG 4215 0.0 20.998446 3 GTATCAA 9135 0.0 20.659952 1 TTTGCGT 230 9.367068E-8 20.578575 3 ACGGTCC 115 0.0038020457 20.57768 8 AGTACTT 3880 0.0 20.310734 12-13 GTGTAGC 715 0.0 19.896193 1 GTACTTT 3980 0.0 19.32473 14-15 GTGTAGG 410 1.8189894E-12 18.505077 1 TATCAAC 10460 0.0 18.0997 2 CATGGGG 2305 0.0 18.069864 4 ATCAACG 10415 0.0 18.04157 3 CAACGCA 10425 0.0 17.933462 5 TCAACGC 10510 0.0 17.833458 4 AACGCAG 10525 0.0 17.761532 6 >>END_MODULE