FastQCFastQC Report
Thu 26 May 2016
SRR938902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152837
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.34636292901771887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.30611439431593535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22890.19855365502668634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.1863229580591185No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17570.1524066281703311No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14280.12386833524600616No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA1401.7936338E-523.725712
GTACATG41600.023.7083051
TACATGG42700.022.7811282
GTATAGG3007.2759576E-1222.2346481
ACATGGG41550.021.8128073
TCGCGCT905.217533E-621.08997760-61
GAGTACT38850.021.00809312-13
GTACTTT40300.019.95785714-15
AGTACTT41350.019.33631112-13
GTATCAA93350.019.2930531
CGGGTCG757.777612E-418.9805714-15
ACACGAC1250.006066899718.980573
CGCGATC1252.1979213E-718.9805722-23
CTCGCGC1051.9741257E-518.07712460-61
CATGGGG22550.017.8863474
TTGCGCG800.001199712917.79428518-19
GGTATCA76050.017.730081
TACTATA4303.6379788E-1217.6563432
ATCAACG105400.016.8826223
CAACGCA105550.016.8136735
Produced by FastQC (version 0.11.5)