##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938902_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1152837 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.163243372653724 33.0 31.0 34.0 30.0 34.0 2 32.39953610094055 34.0 31.0 34.0 31.0 34.0 3 32.49398223686436 34.0 31.0 34.0 30.0 34.0 4 36.06578813830576 37.0 35.0 37.0 35.0 37.0 5 35.977197990696 37.0 35.0 37.0 35.0 37.0 6 36.0160933419035 37.0 35.0 37.0 35.0 37.0 7 35.9607854362759 37.0 35.0 37.0 35.0 37.0 8 35.92597132118417 37.0 35.0 37.0 35.0 37.0 9 37.60592260657838 39.0 37.0 39.0 35.0 39.0 10-11 37.528531353521785 39.0 37.0 39.0 35.0 39.0 12-13 37.45634031523971 39.0 37.0 39.0 34.0 39.0 14-15 38.72010310217316 40.0 38.0 41.0 34.0 41.0 16-17 38.70805413080947 40.0 38.0 41.0 34.0 41.0 18-19 38.81320299400522 40.0 38.0 41.0 34.0 41.0 20-21 38.81492700182246 40.0 38.0 41.0 34.0 41.0 22-23 38.754141739031624 40.0 38.0 41.0 34.0 41.0 24-25 38.69385611322329 40.0 38.0 41.0 34.0 41.0 26-27 38.53279388152878 40.0 38.0 41.0 34.0 41.0 28-29 38.35141438035039 40.0 38.0 41.0 34.0 41.0 30-31 38.18719602164053 40.0 38.0 41.0 33.5 41.0 32-33 38.035097763170334 40.0 37.0 41.0 33.0 41.0 34-35 38.013039571075524 40.0 37.0 41.0 33.0 41.0 36-37 37.90950628753241 40.0 37.0 41.0 33.0 41.0 38-39 37.779731219591326 40.0 37.0 41.0 33.0 41.0 40-41 37.62741133395267 40.0 37.0 41.0 33.0 41.0 42-43 37.46213645120689 40.0 37.0 41.0 32.0 41.0 44-45 37.215249423812736 40.0 37.0 41.0 31.5 41.0 46-47 36.90743574330109 40.0 36.0 41.0 31.0 41.0 48-49 36.616371178232484 39.5 36.0 41.0 30.0 41.0 50-51 36.34222444283103 39.0 35.5 41.0 30.0 41.0 52-53 36.09988706122374 39.0 35.0 41.0 29.0 41.0 54-55 35.8543059426441 39.0 35.0 40.0 28.5 41.0 56-57 35.57813246798983 38.5 35.0 40.0 28.0 41.0 58-59 35.26979139288555 38.0 34.5 40.0 28.0 41.0 60-61 34.857500670086054 38.0 34.0 40.0 26.5 41.0 62-63 34.52794584143292 37.0 34.0 40.0 26.0 41.0 64-65 34.251997897361036 37.0 34.0 39.5 26.0 41.0 66-67 33.802543204286465 36.0 33.0 39.0 25.5 41.0 68-69 33.37992795165318 36.0 33.0 39.0 25.0 40.0 70-71 32.90890819777644 35.0 32.0 38.5 24.0 40.0 72-73 32.38445547809447 35.0 32.0 37.5 22.0 39.5 74-75 31.910348123802414 35.0 31.0 37.0 21.5 39.0 76-77 29.927284169401226 33.0 28.5 35.0 18.5 37.5 78-79 30.899140554996066 34.0 30.5 36.0 20.0 38.0 80-81 30.906258213433468 34.0 31.0 35.5 19.5 37.0 82-83 30.57913651279409 34.0 31.0 35.0 18.0 37.0 84-85 30.168384602506684 34.0 30.0 35.0 15.0 36.5 86-87 29.575399210816446 34.0 29.5 35.0 7.0 36.0 88-89 28.990694694913504 34.0 29.0 35.0 2.0 35.5 90-91 28.516107654421226 34.0 29.0 35.0 2.0 35.0 92-93 28.15180463500044 33.0 28.0 35.0 2.0 35.0 94-95 27.737482402108885 33.0 27.0 35.0 2.0 35.0 96-97 27.084706250753577 33.0 26.5 35.0 2.0 35.0 98-99 26.152228372267718 33.0 25.0 34.0 2.0 35.0 100-101 21.691108109819517 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07064172724672346 1101 2 0.109060286740565 1101 3 0.10324400714733173 1101 4 0.0467116045192455 1101 5 0.13455439622036636 1101 6 0.12071672688816193 1101 7 -0.007106482810030457 1101 8 0.06531550955914867 1101 9 0.11390862565328064 1101 10-11 0.19594429279379 1101 12-13 0.15021055322333154 1101 14-15 0.1235390934296845 1101 16-17 0.1824360017569049 1101 18-19 0.2780728491441664 1101 20-21 0.13095295274850827 1101 22-23 0.20883438384635866 1101 24-25 0.12816021348335482 1101 26-27 0.27851642226139717 1101 28-29 0.3335652339045865 1101 30-31 0.23955921979555939 1101 32-33 0.15333633061853646 1101 34-35 0.13702446744434837 1101 36-37 0.08140658173183368 1101 38-39 0.11063543442300272 1101 40-41 0.21275372646056212 1101 42-43 0.2813263632059204 1101 44-45 0.307732001124414 1101 46-47 0.35496486331428656 1101 48-49 0.23737399027599793 1101 50-51 0.3415529694927031 1101 52-53 0.5573484273400595 1101 54-55 0.4994040925946095 1101 56-57 0.4174109341965533 1101 58-59 0.34651259865977835 1101 60-61 0.4577178612582884 1101 62-63 0.495381676948746 1101 64-65 0.21003503005255197 1101 66-67 0.39768295088357064 1101 68-69 0.3635737365024738 1101 70-71 0.6077581569048007 1101 72-73 0.48456798056582784 1101 74-75 0.3923771198174677 1101 76-77 0.28062591565687356 1101 78-79 0.5669679938972898 1101 80-81 0.7062534428898815 1101 82-83 0.5922452974881764 1101 84-85 0.41069566991241757 1101 86-87 0.7520754813605102 1101 88-89 0.6885253963256588 1101 90-91 0.7959529626146526 1101 92-93 0.8348195708175759 1101 94-95 0.8855273040714415 1101 96-97 0.8759478472025073 1101 98-99 0.747412851547189 1101 100-101 1.1907622885148932 1102 1 0.128456881850866 1102 2 0.11846395646533381 1102 3 0.077369049620998 1102 4 0.10419546452603612 1102 5 0.14958157936681715 1102 6 0.04706738947735545 1102 7 0.08050819608904902 1102 8 0.1449281497222472 1102 9 0.2623710789322473 1102 10-11 0.2930136098172369 1102 12-13 0.2703175868624754 1102 14-15 0.22218843084049666 1102 16-17 0.25606834970117376 1102 18-19 0.20263112301436337 1102 20-21 0.3032643696700106 1102 22-23 0.1030707073258057 1102 24-25 0.22007747478134831 1102 26-27 0.3671079954859948 1102 28-29 0.4489024748152204 1102 30-31 0.26913363365896004 1102 32-33 0.25014231091075345 1102 34-35 0.3565326223882863 1102 36-37 0.3780171831589456 1102 38-39 0.513769990997929 1102 40-41 0.4727775117137085 1102 42-43 0.3237575558664716 1102 44-45 0.3098556844407554 1102 46-47 0.32955286875091616 1102 48-49 0.1776347785872403 1102 50-51 0.21722830078730482 1102 52-53 0.3467257633917029 1102 54-55 0.3620442556934833 1102 56-57 0.21629812413878824 1102 58-59 0.07021715183680755 1102 60-61 0.058783950283093134 1102 62-63 0.10015571704388293 1102 64-65 0.2647241228150463 1102 66-67 0.14766596141704014 1102 68-69 0.43353635967611126 1102 70-71 0.35286943791057723 1102 72-73 0.20007086877514269 1102 74-75 0.3003281901538628 1102 76-77 0.06545007467828157 1102 78-79 0.06947818757720725 1102 80-81 0.27449163521064435 1102 82-83 0.1576802278313636 1102 84-85 0.22910375689848905 1102 86-87 0.27208907293373485 1102 88-89 0.48822808066137213 1102 90-91 0.3988326771916171 1102 92-93 0.5522974845110831 1102 94-95 0.5407303281964815 1102 96-97 0.6675380612697843 1102 98-99 0.6970178464503505 1102 100-101 0.8356696959222951 1103 1 0.10142120646960251 1103 2 0.1430963094065092 1103 3 0.1576658905796009 1103 4 0.09010913555000144 1103 5 -0.039851971831446065 1103 6 -0.05456659745075143 1103 7 0.07738319608905186 1103 8 0.15630478370917444 1103 9 -0.014337799063390833 1103 10-11 0.18258673073226817 1103 12-13 0.08012150843109822 1103 14-15 0.1306238883568298 1103 16-17 0.1372196296576007 1103 18-19 0.08432706528016354 1103 20-21 0.07573168993145174 1103 22-23 0.14353571277242594 1103 24-25 0.11681290506457742 1103 26-27 -0.06556833893665726 1103 28-29 0.11059909790890288 1103 30-31 0.2642725225478486 1103 32-33 0.15830884141184498 1103 34-35 0.1377724099699904 1103 36-37 0.09792118642692316 1103 38-39 0.1954489125665475 1103 40-41 0.14856727206228726 1103 42-43 0.20087152645470496 1103 44-45 0.31284111472398735 1103 46-47 0.47951542321714413 1103 48-49 0.3455507644260294 1103 50-51 0.3041870426173645 1103 52-53 0.36185716317384475 1103 54-55 0.48399074262159303 1103 56-57 0.408182655729199 1103 58-59 0.3402811496581606 1103 60-61 0.40269054069703714 1103 62-63 0.2899875525558713 1103 64-65 0.33449536464510743 1103 66-67 0.2781023720924196 1103 68-69 0.28867661022076874 1103 70-71 0.2401515729868322 1103 72-73 0.4406788480779724 1103 74-75 0.5161063764283682 1103 76-77 0.1661240942861255 1103 78-79 0.4984406058778603 1103 80-81 0.678440436953565 1103 82-83 0.821412526306311 1103 84-85 0.6999200695346559 1103 86-87 0.36211494439343284 1103 88-89 0.5864443115981928 1103 90-91 0.7598518765380256 1103 92-93 0.7641166566243776 1103 94-95 0.5976815809197937 1103 96-97 0.25414958087762685 1103 98-99 0.43207707847649246 1103 100-101 0.6166814061619483 1104 1 0.09231418619290821 1104 2 0.0755106351008834 1104 3 0.0821200304042975 1104 4 0.061434975131795966 1104 5 0.0017442896534021202 1104 6 -0.042210488041099836 1104 7 0.0035782140423705755 1104 8 -0.04954621692446892 1104 9 0.03525990132735757 1104 10-11 0.10473812607497024 1104 12-13 0.03852524694625714 1104 14-15 0.1226422509083207 1104 16-17 0.12529502700684958 1104 18-19 0.053247852850120125 1104 20-21 0.09995547280187367 1104 22-23 0.019013422931891455 1104 24-25 -0.10509790257086848 1104 26-27 -0.01738066732403354 1104 28-29 -0.0470040016792268 1104 30-31 0.007371714648385819 1104 32-33 -0.10467183896623311 1104 34-35 -0.06339307080940415 1104 36-37 -0.03988128083466336 1104 38-39 0.08610691499552559 1104 40-41 0.25002333922883224 1104 42-43 0.10261751995982848 1104 44-45 0.024334705652272248 1104 46-47 0.09676727795365281 1104 48-49 0.15716217402577115 1104 50-51 -0.044129668492566054 1104 52-53 -0.0011489290745529956 1104 54-55 0.10958066103958686 1104 56-57 -0.15441635155773525 1104 58-59 0.05015514638431995 1104 60-61 0.22830735609465336 1104 62-63 0.35680509532701876 1104 64-65 0.2818894928529474 1104 66-67 0.2825519245794865 1104 68-69 0.22251169048267627 1104 70-71 0.0018080309021328844 1104 72-73 -0.18732024765547095 1104 74-75 0.04371538345128556 1104 76-77 0.13918586163220326 1104 78-79 0.16857802799212962 1104 80-81 0.267408384994539 1104 82-83 0.33999454394280804 1104 84-85 -0.014451333200586447 1104 86-87 5.716848095254079E-4 1104 88-89 0.2969371038677018 1104 90-91 0.5098989049993179 1104 92-93 0.35241135511418165 1104 94-95 0.56561147055967 1104 96-97 0.41652070639246475 1104 98-99 0.36323318656604897 1104 100-101 0.7160764716387682 1105 1 0.07262272911405887 1105 2 0.04479139009365696 1105 3 0.017208446540038835 1105 4 -0.04119129391381904 1105 5 0.007193837642788026 1105 6 0.18900445929052978 1105 7 0.03758784210675259 1105 8 0.15653090291792182 1105 9 0.09943269807351385 1105 10-11 0.07396247904043918 1105 12-13 0.06803145116926146 1105 14-15 0.1698531309388187 1105 16-17 0.09120437067790732 1105 18-19 -0.057406569545449315 1105 20-21 0.007603111066273982 1105 22-23 0.04812560735857829 1105 24-25 0.0015384711759764969 1105 26-27 -0.048665521393715494 1105 28-29 -0.029493952585632144 1105 30-31 -0.0062473889565737295 1105 32-33 0.011564795342088985 1105 34-35 0.11996674234895721 1105 36-37 0.18153242406356895 1105 38-39 0.22210332433125757 1105 40-41 0.23593713151048945 1105 42-43 0.011146936397452123 1105 44-45 -0.03401517757169614 1105 46-47 -0.03549547605407355 1105 48-49 -0.027605306630711368 1105 50-51 0.12392692418946893 1105 52-53 0.11875542579747389 1105 54-55 0.10816028322543758 1105 56-57 0.1137006151253459 1105 58-59 0.17845625377059804 1105 60-61 0.36603157401302155 1105 62-63 0.049254373222190395 1105 64-65 0.045648409934024414 1105 66-67 -0.08234059381075554 1105 68-69 -0.12026567244451059 1105 70-71 0.06273095872342793 1105 72-73 0.14919410705766722 1105 74-75 0.1332190456110851 1105 76-77 -0.2166664444957611 1105 78-79 0.097636987970521 1105 80-81 0.11838707932732717 1105 82-83 0.15182149299032943 1105 84-85 0.08235678869134588 1105 86-87 -0.0801888687247363 1105 88-89 -0.027152122550688773 1105 90-91 0.13892608918768445 1105 92-93 0.39985975917512917 1105 94-95 0.26519361892083637 1105 96-97 0.5187180022727382 1105 98-99 0.6471530480236076 1105 100-101 1.576160518603384 1106 1 0.06783310017445388 1106 2 -0.08548341195571396 1106 3 0.01616047262294984 1106 4 -0.14224703060066446 1106 5 -0.10241647131545051 1106 6 -0.04094430642907554 1106 7 -0.030895312682879705 1106 8 0.0022380912427166777 1106 9 -0.0709135214576122 1106 10-11 -0.041050717668142056 1106 12-13 0.033914078090546695 1106 14-15 0.06765626709780292 1106 16-17 0.0038266343015678217 1106 18-19 0.015691551864264852 1106 20-21 -0.05324147828320491 1106 22-23 0.08569741297882416 1106 24-25 -0.01732505933171069 1106 26-27 -0.03542611757443126 1106 28-29 0.10609058397700721 1106 30-31 0.10868041728468825 1106 32-33 0.028992213444873016 1106 34-35 0.18102092648185675 1106 36-37 -0.0012225829229208784 1106 38-39 0.09227876292774795 1106 40-41 0.002553340173740537 1106 42-43 0.14261720498927843 1106 44-45 0.0928478871698033 1106 46-47 0.09112694282498524 1106 48-49 -0.1568437789176258 1106 50-51 -0.09936526646415444 1106 52-53 -0.012509129395816387 1106 54-55 -0.0324001887509624 1106 56-57 0.09154178885614073 1106 58-59 0.06580682045278508 1106 60-61 0.07898375535132374 1106 62-63 -0.10617956560718511 1106 64-65 -0.09550979531361037 1106 66-67 0.10127219923379727 1106 68-69 0.11331218813614896 1106 70-71 -0.13575141978923 1106 72-73 0.10060693115330821 1106 74-75 0.21918783927667107 1106 76-77 -0.14047097795329577 1106 78-79 0.3296390062906589 1106 80-81 0.23740586523013718 1106 82-83 0.37878159235378206 1106 84-85 0.10946438068016207 1106 86-87 0.06209881952242924 1106 88-89 0.1228284705249223 1106 90-91 0.3397683497062367 1106 92-93 0.2656259445500737 1106 94-95 0.18763578628615107 1106 96-97 0.0793801665329461 1106 98-99 0.38744182305853414 1106 100-101 0.28111316570787537 1107 1 0.04114093192546164 1107 2 0.06281597047584597 1107 3 0.00999861438716465 1107 4 -0.049286081818905814 1107 5 -0.07244443088161034 1107 6 0.008120123472295404 1107 7 -0.07982455347311657 1107 8 0.11881411975532785 1107 9 0.062100273976682274 1107 10-11 0.06622337825467639 1107 12-13 0.08381729497688895 1107 14-15 0.08639839386831483 1107 16-17 0.06385520836369807 1107 18-19 0.08427314156531907 1107 20-21 0.07718682540069466 1107 22-23 0.1328000645288867 1107 24-25 0.11939721921930868 1107 26-27 0.11693124255092613 1107 28-29 0.032572287359812435 1107 30-31 0.1689901232483706 1107 32-33 0.18646909303438264 1107 34-35 0.05326107150289516 1107 36-37 0.06637581324262243 1107 38-39 0.1139597166254731 1107 40-41 0.07282940248364156 1107 42-43 -0.012538416112512607 1107 44-45 -0.16076765343301958 1107 46-47 -0.011310301775132814 1107 48-49 -0.1015967515418339 1107 50-51 -0.010892442547977055 1107 52-53 0.04119858560095935 1107 54-55 -0.09734473049059744 1107 56-57 -0.047519414941447735 1107 58-59 -0.011827830974375786 1107 60-61 -0.06845302837682965 1107 62-63 -0.35515335735450293 1107 64-65 -0.2941102807654232 1107 66-67 -0.2260513636137702 1107 68-69 -0.22645261353528667 1107 70-71 -0.34814810765600157 1107 72-73 -0.27371942765088164 1107 74-75 -0.1584575658297922 1107 76-77 -0.28765120961827506 1107 78-79 -0.12217145243057459 1107 80-81 -0.10086187065950014 1107 82-83 -0.09749425488252328 1107 84-85 -0.06616931827826278 1107 86-87 -8.109724706848453E-4 1107 88-89 0.06608207300748603 1107 90-91 0.21734308777733347 1107 92-93 0.29457792169082 1107 94-95 0.2717618194074838 1107 96-97 0.2519805598103595 1107 98-99 -0.0015202932655462575 1107 100-101 0.8786782254676062 1108 1 -0.08043468119232244 1108 2 0.005918830111546924 1108 3 -0.04648440672342957 1108 4 -0.049812424229145336 1108 5 -0.022677412477015935 1108 6 -0.039595265957864 1108 7 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-1.6203079830609113 2316 58-59 -1.1830984452606614 2316 60-61 -1.1076018364504705 2316 62-63 -1.0839437137780479 2316 64-65 -1.249300316773855 2316 66-67 -1.2633372486393384 2316 68-69 -1.2303356696729821 2316 70-71 -1.1684481014058683 2316 72-73 -1.1639609890112652 2316 74-75 -1.5583632398048692 2316 76-77 -1.6834786701626996 2316 78-79 -1.7154949045972678 2316 80-81 -1.6130343284806514 2316 82-83 -1.6366354097289957 2316 84-85 -1.9457681105990154 2316 86-87 -2.081715788008779 2316 88-89 -2.377871431086355 2316 90-91 -2.369863203685618 2316 92-93 -2.621950242822951 2316 94-95 -2.663143656859223 2316 96-97 -3.0924886442412607 2316 98-99 -3.425877071409971 2316 100-101 -3.788006830203244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 6.0 5 23.0 6 57.0 7 145.0 8 341.0 9 617.0 10 987.0 11 1529.0 12 2308.0 13 3073.0 14 3820.0 15 5175.0 16 6893.0 17 9557.0 18 9574.0 19 6809.0 20 6254.0 21 6564.0 22 7249.0 23 7905.0 24 9135.0 25 10921.0 26 13207.0 27 15694.0 28 19456.0 29 23507.0 30 28506.0 31 34616.0 32 42741.0 33 54607.0 34 72922.0 35 104391.0 36 165810.0 37 260570.0 38 202381.0 39 15484.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.153051830471185 19.84127467998436 15.28166689457012 22.724006594974338 2 15.411632346984005 20.919002426188612 39.97590292469794 23.693462302129443 3 19.20401583224688 24.74061814462929 30.495377924199175 25.559988098924652 4 13.262412639427776 17.43004431675944 37.01407918031777 32.29346386349501 5 13.633371008636983 37.5164486096425 34.46370809995897 14.38647228176154 6 31.41571618537573 36.596240405191715 17.29758847087663 14.690454938555927 7 27.672775943173235 33.1688694932588 21.063602226507303 18.09475233706066 8 25.397432594547194 35.72768743543103 19.702178191713138 19.172701778308642 9 25.687933333159847 17.483217488682268 20.747685926110975 36.081163252046906 10-11 24.730165669561266 26.69006980171525 27.36670492012314 21.21305960860035 12-13 25.81670262144605 24.10791811851979 27.798335757787097 22.277043502247064 14-15 22.74132317395549 25.577222591604198 26.422015802030923 25.259438432409386 16-17 21.80817150426219 28.591460352130575 27.215562655109988 22.384805488497246 18-19 21.619665225873216 27.622508646061846 29.238565382616972 21.51926074544797 20-21 22.715613742445807 26.572273443687184 29.010866236944167 21.701246576922845 22-23 22.287192378454197 26.46254414110581 28.99035162820069 22.259911852239302 24-25 22.212290202344303 26.607924624209666 29.176978185120706 22.00280698832532 26-27 22.191515366005778 26.504961239099718 29.325438028099377 21.978085366795135 28-29 22.02501307643665 26.905711735483855 29.25756199705596 21.811713191023536 30-31 22.261126247682892 26.65992677195475 28.984496507311963 22.094450473050397 32-33 22.136910942310145 26.71596244742318 29.01260108757786 22.13452552268881 34-35 22.424476108027836 26.72785896076904 28.867492934679834 21.98017199652329 36-37 22.50284317391029 26.545875511055634 29.078534058664374 21.872747256369706 38-39 22.36971663894911 26.681379961290848 29.094629213697594 21.85427418606245 40-41 22.322388183992317 26.744196133097503 28.98697876231015 21.94643692060003 42-43 22.1080489821127 26.918346086019696 28.662533645492953 22.31107128637465 44-45 22.30174608909266 26.906530734923994 28.476401839467712 22.315321336515638 46-47 22.53500174135859 27.034342714116626 28.112206815282843 22.318448729241943 48-49 22.5702486220913 26.982679206199677 28.122565781370085 22.32450639033894 50-51 22.454519628827256 27.337015568176643 28.02406247221517 22.184402330780927 52-53 22.605884439864436 27.41150743773838 27.78046679626001 22.202141326137173 54-55 22.295476290230102 27.171881193958903 27.899347435934136 22.633295079876863 56-57 22.42346489573114 27.45596298522688 27.633698432649194 22.486873686392787 58-59 22.624443220408818 27.214128648071927 27.70136229382349 22.460065837695765 60-61 22.46571765758037 27.11212061937004 28.11982652920044 22.302335193849153 62-63 22.410236659649197 27.139569600906288 28.073786667152422 22.376407072292093 64-65 22.302541126874186 27.224277541885872 28.03063751534261 22.44254381589733 66-67 22.276649073598993 27.226201107456795 27.758764041093958 22.738385777850254 68-69 22.10630819435505 27.31237714886478 27.930341129302285 22.650973527477884 70-71 22.415180983955235 27.109036229753208 27.66206324051015 22.813719545781407 72-73 22.31056949074327 27.061371208592366 27.71701463433252 22.91104466633184 74-75 22.22200536589301 27.551162913751032 27.344195233150913 22.882636487205044 76-77 22.345373315291308 27.065076351458156 27.687760664103962 22.901789669146577 78-79 22.252885707172826 27.14217187685683 27.75353324017186 22.851409175798487 80-81 22.24598967590388 27.271765219194037 27.565692287808254 22.91655281709383 82-83 22.256572264769435 27.17621832054315 27.712937735343328 22.854271679344087 84-85 22.25809025907392 27.119098363428655 27.518504350571675 23.10430702692575 86-87 22.132794595712223 27.093066895088636 27.754051391468575 23.02008711773056 88-89 22.25752403456586 26.97743878744156 27.524827440791412 23.24020973720117 90-91 22.042875278366388 26.879976812411627 27.748640971441578 23.328506937780407 92-93 22.08278360253878 27.091687723416236 27.575190595027742 23.25033807901724 94-95 22.03000077200853 26.907099615990816 27.61231639858887 23.450583213411782 96-97 21.775833967278867 26.788615523670817 27.768072218612755 23.667478290437558 98-99 21.80507738734964 26.90016021345602 27.5651284613523 23.72963393784204 100-101 21.976817110544197 26.225220825566236 28.186172672017545 23.611789391872023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 425.0 1 289.0 2 167.5 3 180.5 4 155.0 5 156.0 6 295.0 7 625.5 8 995.0 9 1205.0 10 1150.5 11 983.0 12 1029.5 13 1164.0 14 1256.5 15 1353.5 16 1435.5 17 1538.0 18 1665.0 19 1826.5 20 2046.5 21 2364.5 22 2868.0 23 3561.0 24 4289.0 25 5240.5 26 6687.5 27 8362.0 28 9981.5 29 11734.5 30 13604.5 31 15762.0 32 17755.0 33 19605.0 34 21743.5 35 24023.0 36 26776.0 37 29371.5 38 30861.0 39 31752.0 40 32668.0 41 33809.5 42 35180.5 43 36767.5 44 38642.5 45 40313.5 46 42042.5 47 45359.5 48 52800.5 49 52674.5 50 45052.0 51 41921.5 52 40810.0 53 40150.0 54 39043.5 55 36475.0 56 32584.5 57 29097.5 58 26544.0 59 23319.5 60 19568.0 61 15833.0 62 12554.5 63 9748.5 64 6999.5 65 4822.5 66 3356.5 67 2588.0 68 1932.0 69 1388.0 70 993.5 71 659.5 72 440.0 73 281.0 74 168.5 75 77.0 76 43.5 77 22.5 78 12.5 79 9.0 80 4.0 81 3.0 82 2.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40682247360207907 2 0.0 3 0.0 4 0.0 5 3.4697012673951303E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.107965826912217E-4 16-17 0.002255305823806835 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0019950782287522 36-37 0.006592432408050748 38-39 0.0119704693725132 40-41 0.0024287908871765914 42-43 6.939402534790261E-4 44-45 3.0359886089707393E-4 46-47 1.3011379752731739E-4 48-49 2.6022759505463477E-4 50-51 3.9034139258195213E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7348506336975651E-4 60-61 0.0017782218995400045 62-63 0.0 64-65 1.7348506336975651E-4 66-67 0.0013445092411156132 68-69 0.001648108102012687 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12586341347475835 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15188617298022183 88-89 0.44368804956815233 90-91 0.34232940129437206 92-93 0.0 94-95 0.0 96-97 3.4697012673951303E-4 98-99 0.0 100-101 3.4697012673951303E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1152837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.23773144290831 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37378345520287 52.43403300100993 2 11.929453666672584 15.56500989134008 3 3.4632262284857678 6.777990678599721 4 1.507719926651534 3.9344091066405684 5 0.7885642049001187 2.5722069912382235 6 0.47635547193563743 1.8645810209698177 7 0.30200424449883967 1.3791450258063607 8 0.22474258619179596 1.1729357185412048 9 0.15916847771566076 0.9345411363051732 >10 0.7278661544082993 8.221060698289422 >50 0.032315005418242136 1.4522734479151018 >100 0.012000472445224978 1.4954805776508413 >500 0.0018667369823179509 0.7814904759772858 >1k 9.333684911589754E-4 1.4148422297162238 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3993 0.34636292901771887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3529 0.30611439431593535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2289 0.19855365502668634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2148 0.1863229580591185 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1757 0.1524066281703311 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1428 0.12386833524600616 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.674253168487826E-5 0.0 5 0.0 0.0 0.0 8.674253168487826E-5 0.0 6 0.0 0.0 0.0 8.674253168487826E-5 0.0 7 8.674253168487826E-5 0.0 0.0 8.674253168487826E-5 0.0 8 8.674253168487826E-5 0.0 0.0 8.674253168487826E-5 0.0 9 8.674253168487826E-5 0.0 0.0 1.7348506336975651E-4 0.0 10-11 1.7348506336975651E-4 0.0 0.0 1.7348506336975651E-4 0.0 12-13 1.7348506336975651E-4 0.0 0.0 2.1685632921219564E-4 0.0 14-15 1.7348506336975651E-4 0.0 0.0 2.6022759505463477E-4 0.0 16-17 1.7348506336975651E-4 0.0 0.0 3.4697012673951303E-4 0.0 18-19 1.7348506336975651E-4 0.0 0.0 4.337126584243913E-4 0.0 20-21 1.7348506336975651E-4 0.0 0.0 5.204551901092695E-4 0.0 22-23 1.7348506336975651E-4 0.0 0.0 7.373115193214652E-4 0.0 24-25 3.4697012673951303E-4 0.0 0.0 0.0011276529119034174 0.0 26-27 3.4697012673951303E-4 0.0 0.0 0.0015613655703278087 0.0 28-29 4.337126584243913E-4 0.0 0.0 0.0032528449381829345 0.0 30-31 4.337126584243913E-4 0.0 0.0 0.009801906080391243 0.0 32-33 4.337126584243913E-4 0.0 0.0 0.02229283064301371 0.0 34-35 4.770839242668304E-4 0.0 0.0 0.04072561862605034 0.0 36-37 6.071977217941479E-4 0.0 0.0 0.06909042648700553 0.0 38-39 6.071977217941479E-4 0.0 0.0 0.10860164966946759 0.0 40-41 6.071977217941479E-4 0.0 0.0 0.1514958315876399 0.0 42-43 6.071977217941479E-4 0.0 0.0 0.19564778021524293 0.0 44-45 8.674253168487826E-4 0.0 0.0 0.2531147074564748 0.0 46-47 9.541678485336609E-4 0.0 0.0 0.3259784340717725 0.0 48-49 0.0012143954435882957 0.0 0.0 0.4033094010688415 0.0 50-51 0.0012143954435882957 0.0 0.0 0.4836329854090387 0.0 52-53 0.0012143954435882957 0.0 0.0 0.5689442653211165 0.0 54-55 0.0012143954435882957 0.0 0.0 0.6643176789086402 0.0 56-57 0.001301137975273174 0.0 0.0 0.7814634679490683 0.0 58-59 0.001301137975273174 0.0 0.0 0.9119242356031252 0.0 60-61 0.001301137975273174 0.0 0.0 1.0557433531366534 0.0 62-63 0.001301137975273174 0.0 0.0 1.208193352572827 0.0 64-65 0.001301137975273174 0.0 0.0 1.3707488569502888 0.0 66-67 0.0013878805069580521 0.0 0.0 1.556551359819298 0.0 68-69 0.0013878805069580521 0.0 0.0 1.7688970773838801 0.0 70-71 0.0013878805069580521 0.0 0.0 1.997550390905219 0.0 72-73 0.0014312517728004913 0.0 0.0 2.2409499348129875 0.0 74-75 0.0014746230386429304 0.0 0.0 2.499269194170555 0.0 76-77 0.0014746230386429304 0.0 0.0 2.781746248602361 0.0 78-79 0.0014746230386429304 0.0 0.0 3.0963614110234143 0.0 80-81 0.0015613655703278087 0.0 0.0 3.4482758620689653 0.0 82-83 0.0015613655703278087 0.0 0.0 3.8069996018517793 0.0 84-85 0.0015613655703278087 0.0 0.0 4.187148746960759 0.0 86-87 0.0015613655703278087 0.0 0.0 4.599219143729773 0.0 88-89 0.0015613655703278087 0.0 0.0 5.0375725275993055 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGGA 140 1.7936338E-5 23.72571 2 GTACATG 4160 0.0 23.708305 1 TACATGG 4270 0.0 22.781128 2 GTATAGG 300 7.2759576E-12 22.234648 1 ACATGGG 4155 0.0 21.812807 3 TCGCGCT 90 5.217533E-6 21.089977 60-61 GAGTACT 3885 0.0 21.008093 12-13 GTACTTT 4030 0.0 19.957857 14-15 AGTACTT 4135 0.0 19.336311 12-13 GTATCAA 9335 0.0 19.293053 1 CGGGTCG 75 7.777612E-4 18.98057 14-15 ACACGAC 125 0.0060668997 18.98057 3 CGCGATC 125 2.1979213E-7 18.98057 22-23 CTCGCGC 105 1.9741257E-5 18.077124 60-61 CATGGGG 2255 0.0 17.886347 4 TTGCGCG 80 0.0011997129 17.794285 18-19 GGTATCA 7605 0.0 17.73008 1 TACTATA 430 3.6379788E-12 17.656343 2 ATCAACG 10540 0.0 16.882622 3 CAACGCA 10555 0.0 16.813673 5 >>END_MODULE