##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938901_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594490 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880830627933186 33.0 31.0 34.0 30.0 34.0 2 31.954897475146765 33.0 31.0 34.0 30.0 34.0 3 31.967195411192787 34.0 31.0 34.0 30.0 34.0 4 35.49729516055779 37.0 35.0 37.0 33.0 37.0 5 35.553795690423726 37.0 35.0 37.0 33.0 37.0 6 35.671647967165136 37.0 35.0 37.0 33.0 37.0 7 35.636592709717576 37.0 35.0 37.0 33.0 37.0 8 35.64205285202443 37.0 35.0 37.0 33.0 37.0 9 37.227490790425406 39.0 37.0 39.0 34.0 39.0 10-11 37.14473330081246 39.0 37.0 39.0 33.5 39.0 12-13 37.084768456996756 39.0 37.0 39.0 33.0 39.0 14-15 38.20156100186715 40.0 38.0 41.0 33.0 41.0 16-17 38.11765546939394 40.0 38.0 41.0 33.0 41.0 18-19 38.117326616091106 40.0 38.0 41.0 33.5 41.0 20-21 38.21699608067419 40.0 38.0 41.0 34.0 41.0 22-23 38.16574626991203 40.0 38.0 41.0 33.5 41.0 24-25 38.075404968964996 40.0 38.0 41.0 33.0 41.0 26-27 37.881887836633084 40.0 37.5 41.0 33.0 41.0 28-29 37.75825665696647 40.0 37.0 41.0 33.0 41.0 30-31 37.609660381167046 40.0 37.0 41.0 33.0 41.0 32-33 37.40098824202258 40.0 36.0 41.0 33.0 41.0 34-35 37.09309744486871 40.0 36.0 41.0 31.5 41.0 36-37 36.77740163837912 39.0 35.0 41.0 31.0 41.0 38-39 36.48475836431227 39.0 35.0 41.0 30.5 41.0 40-41 36.37998031926526 39.0 35.0 41.0 30.0 41.0 42-43 36.23534121684132 39.0 35.0 41.0 30.0 41.0 44-45 36.12991135258793 39.0 35.0 41.0 30.0 41.0 46-47 35.84438594425474 39.0 35.0 41.0 28.5 41.0 48-49 35.58251778835641 39.0 35.0 41.0 27.0 41.0 50-51 34.481209944658445 38.0 34.0 40.0 24.5 40.5 52-53 34.5849938602836 38.5 34.5 39.5 25.0 40.5 54-55 35.14719591582701 39.0 35.0 40.5 26.0 41.0 56-57 35.157280189742465 39.0 35.0 41.0 25.5 41.0 58-59 35.040882941681105 39.0 35.0 41.0 25.0 41.0 60-61 34.73015441807263 39.0 35.0 40.5 23.5 41.0 62-63 34.36997678682569 38.0 34.5 40.0 22.5 41.0 64-65 33.94955255765446 38.0 34.0 40.0 22.0 41.0 66-67 33.504158186008176 37.0 33.5 39.5 21.0 41.0 68-69 33.09386280677556 36.5 33.0 39.0 19.5 41.0 70-71 32.56902639236993 36.0 32.5 39.0 16.5 40.0 72-73 32.05683442951101 35.5 32.0 38.0 12.5 40.0 74-75 31.576230045921715 35.0 31.5 37.0 8.0 39.5 76-77 31.094113441773622 35.0 31.0 37.0 4.5 39.0 78-79 30.648787195747616 35.0 31.0 36.0 2.0 39.0 80-81 30.206497165637774 35.0 31.0 36.0 2.0 37.5 82-83 29.71869669801006 34.0 30.0 35.5 2.0 37.0 84-85 29.26971774125721 34.0 30.0 35.0 2.0 37.0 86-87 28.829092163030495 34.0 29.0 35.0 2.0 36.0 88-89 28.249814967451094 34.0 28.5 35.0 2.0 36.0 90-91 27.717027199784688 34.0 27.5 35.0 2.0 35.5 92-93 27.278558091809785 34.0 26.0 35.0 2.0 35.0 94-95 26.76420293024273 34.0 25.5 35.0 2.0 35.0 96-97 26.068289626402464 33.0 24.5 35.0 2.0 35.0 98-99 25.296931823916296 33.0 19.0 35.0 2.0 35.0 100-101 22.224966778246902 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1809127385576481 1101 2 0.18216296756009953 1101 3 0.27642361288235406 1101 4 0.13616386510486223 1101 5 -0.033024952434040244 1101 6 0.2530241691525603 1101 7 0.058185546496140716 1101 8 0.13213143732735233 1101 9 0.24999008676606138 1101 10-11 0.16277425400870982 1101 12-13 0.011621986047600785 1101 14-15 0.3371034386026821 1101 16-17 0.4338513129716901 1101 18-19 0.5547042077769433 1101 20-21 0.33749928513032756 1101 22-23 0.20656293841220474 1101 24-25 0.26772188823206733 1101 26-27 0.4344255560438981 1101 28-29 0.3533186711674432 1101 30-31 0.11645360520970627 1101 32-33 0.28494183345414825 1101 34-35 0.4521164982365349 1101 36-37 0.32164392544466125 1101 38-39 0.33576060792375273 1101 40-41 0.17832315131582277 1101 42-43 0.2058587610082796 1101 44-45 0.2741150873215048 1101 46-47 0.3124513532806503 1101 48-49 0.3144831862191495 1101 50-51 0.34552436427772903 1101 52-53 0.4007213151911486 1101 54-55 0.3073669544101634 1101 56-57 0.3651101700021897 1101 58-59 0.4544309451422919 1101 60-61 0.4795242237591921 1101 62-63 0.6407838281413376 1101 64-65 0.6905701203023398 1101 66-67 0.6523664552332065 1101 68-69 0.7369473772626591 1101 70-71 0.757818976223831 1101 72-73 0.6907984736739863 1101 74-75 0.5156661656909591 1101 76-77 0.3230658397853041 1101 78-79 0.3303424597398461 1101 80-81 0.25995596630522755 1101 82-83 0.3947311970383254 1101 84-85 0.39267355388708935 1101 86-87 0.22392726989347267 1101 88-89 -0.07318387229851098 1101 90-91 -0.15101514022897433 1101 92-93 0.03407729305120455 1101 94-95 -0.05486254366910259 1101 96-97 -0.4461499549018555 1101 98-99 -0.34764954427800276 1101 100-101 0.3646734301357917 1102 1 0.12116260653619193 1102 2 -0.06024232441577482 1102 3 0.21259123844171057 1102 4 0.017601648256146518 1102 5 -0.07873911903121922 1102 6 -0.044847505991270964 1102 7 0.24489862912767535 1102 8 0.0747298975402586 1102 9 0.14847948335851413 1102 10-11 0.2785136436526656 1102 12-13 0.32234317062749085 1102 14-15 0.281965371952694 1102 16-17 0.29025887015782104 1102 18-19 0.06360523337520618 1102 20-21 0.20695090968909824 1102 22-23 0.2569638667314962 1102 24-25 0.1775842384919386 1102 26-27 0.18325473417558413 1102 28-29 0.3185501256269916 1102 30-31 0.12429672202784303 1102 32-33 0.1941562363002518 1102 34-35 0.1679194666347641 1102 36-37 0.04105663841611573 1102 38-39 0.25769597299782276 1102 40-41 0.019688948030569975 1102 42-43 -0.30354255642689765 1102 44-45 0.12131241076018284 1102 46-47 0.16847848021581058 1102 48-49 0.04066894735758808 1102 50-51 -0.08549616158457951 1102 52-53 -0.15261187917223396 1102 54-55 -0.05969959830114391 1102 56-57 -0.10172986168296205 1102 58-59 -0.15384923218336155 1102 60-61 0.0031056879466362375 1102 62-63 0.0017669710858427834 1102 64-65 -0.007354363424283861 1102 66-67 -0.07536534665122474 1102 68-69 0.08429756765149676 1102 70-71 0.10241061301195487 1102 72-73 0.32694907541388574 1102 74-75 0.17447755566844236 1102 76-77 0.39467705744005244 1102 78-79 0.06293994716306628 1102 80-81 0.06893134082925201 1102 82-83 -0.044273152721252984 1102 84-85 0.0364275492732844 1102 86-87 0.008530788612723939 1102 88-89 0.10316031458828334 1102 90-91 0.02084726561888317 1102 92-93 0.076067064862535 1102 94-95 -0.13741938238057472 1102 96-97 -0.12442234991005208 1102 98-99 -0.3812854846876874 1102 100-101 -0.5333174117441963 1103 1 0.18418275212616564 1103 2 0.24506663106077653 1103 3 0.30934762916458425 1103 4 0.19370796279821434 1103 5 -0.02698696057515093 1103 6 0.15411643509557393 1103 7 0.10311091963046692 1103 8 0.07114093529207821 1103 9 0.27697787509713123 1103 10-11 0.12391740190559375 1103 12-13 0.15889810545058225 1103 14-15 0.2815980111942622 1103 16-17 0.3892549764723654 1103 18-19 0.454554954045598 1103 20-21 0.43029440046275624 1103 22-23 0.45822508223852765 1103 24-25 0.4492585232388535 1103 26-27 0.38660330366940343 1103 28-29 0.34260971594355993 1103 30-31 0.24093460928025223 1103 32-33 0.3512579799941662 1103 34-35 0.22285598263272988 1103 36-37 0.28263442747994105 1103 38-39 0.22414256179078507 1103 40-41 0.0881196235003543 1103 42-43 0.3200785710489811 1103 44-45 0.4845323193432165 1103 46-47 0.05810942655067208 1103 48-49 -0.02467217334665861 1103 50-51 -0.16067984196379115 1103 52-53 0.008977760238636279 1103 54-55 -0.04646276065089694 1103 56-57 0.06655860962226967 1103 58-59 -0.005660642374664349 1103 60-61 0.32004321968863536 1103 62-63 0.21782249842628687 1103 64-65 -0.20942309543714543 1103 66-67 -0.04506570216163652 1103 68-69 -0.10690947484047086 1103 70-71 -0.204653886734107 1103 72-73 -0.3981465738158434 1103 74-75 -0.3689810527622299 1103 76-77 0.10738062947323002 1103 78-79 0.04337502419032191 1103 80-81 -0.2548133688372438 1103 82-83 -0.3678773511299198 1103 84-85 -0.32597637827030823 1103 86-87 -0.22125590514044902 1103 88-89 -0.10999187772592123 1103 90-91 -0.4181759272031904 1103 92-93 -0.44105160789859355 1103 94-95 -0.33606674991062135 1103 96-97 -0.7656988015775639 1103 98-99 -0.3346949988234549 1103 100-101 0.05813679512900549 1104 1 0.17996269174375001 1104 2 0.21347320603561926 1104 3 0.2973315271853778 1104 4 0.3427921627311221 1104 5 0.2708661088646309 1104 6 0.36973535492113285 1104 7 0.3766164546858164 1104 8 0.3849953465634641 1104 9 0.4546632155868977 1104 10-11 0.4596604411541634 1104 12-13 0.29694238589338795 1104 14-15 0.3755631235726611 1104 16-17 0.3929752045837418 1104 18-19 0.4821564300603569 1104 20-21 0.47748661783948165 1104 22-23 0.4655883831442651 1104 24-25 0.4643574833193682 1104 26-27 0.5483141389595758 1104 28-29 0.3615765056114597 1104 30-31 0.14924054688508903 1104 32-33 0.4445152762034965 1104 34-35 0.32539539893598146 1104 36-37 0.42579108631925777 1104 38-39 0.5082250844341871 1104 40-41 0.33861945577140773 1104 42-43 0.3584583093918141 1104 44-45 0.3463840469513997 1104 46-47 0.5488340156147444 1104 48-49 0.5179343345365979 1104 50-51 0.3994945961442298 1104 52-53 0.34566679076530704 1104 54-55 0.23045102666879558 1104 56-57 0.10097994474471506 1104 58-59 0.07181335963808522 1104 60-61 0.24609152562624814 1104 62-63 0.4435286373058531 1104 64-65 0.5287385952706671 1104 66-67 0.6519017584220563 1104 68-69 0.857173047802938 1104 70-71 0.6946718428868266 1104 72-73 0.7271033423196869 1104 74-75 0.6331759415349865 1104 76-77 0.7007989454745704 1104 78-79 0.538698741063854 1104 80-81 0.4094368827561823 1104 82-83 0.6761481436704813 1104 84-85 0.6291594821792046 1104 86-87 0.7073083350474079 1104 88-89 0.8201389508550925 1104 90-91 0.9103145122466572 1104 92-93 0.8233596634868476 1104 94-95 1.0060265073855845 1104 96-97 1.0448211581674869 1104 98-99 1.0289648468659074 1104 100-101 1.3244031487016308 1105 1 0.3214733047108389 1105 2 0.040824206818353304 1105 3 0.2408627806797341 1105 4 0.1598755207304805 1105 5 0.1475228433464153 1105 6 0.15511465256437873 1105 7 0.02186314779445553 1105 8 0.08468817236872184 1105 9 -0.19525028889574259 1105 10-11 0.1490510917451644 1105 12-13 0.1487555029550407 1105 14-15 0.18161583650621083 1105 16-17 0.2311266342263778 1105 18-19 0.4602234032434609 1105 20-21 0.416622386202512 1105 22-23 0.19432133892301806 1105 24-25 0.3317184162869822 1105 26-27 0.2722717528672689 1105 28-29 0.2931266499899081 1105 30-31 0.34068505491305956 1105 32-33 0.3984950566430143 1105 34-35 0.5860823640944091 1105 36-37 0.46712640608956235 1105 38-39 0.48813543166600937 1105 40-41 0.5808825468515195 1105 42-43 0.5450518330810681 1105 44-45 0.5194342801275269 1105 46-47 0.5587333124686751 1105 48-49 0.3894276484002148 1105 50-51 0.17627202969396194 1105 52-53 0.19038595988212847 1105 54-55 0.2862110361405499 1105 56-57 0.24153187165435952 1105 58-59 0.2464783950584888 1105 60-61 0.37093448528579387 1105 62-63 0.47714513657244595 1105 64-65 -0.028032721105603287 1105 66-67 0.012795260405212616 1105 68-69 -0.12815724667647288 1105 70-71 -0.24281787960398304 1105 72-73 -0.09472411392279412 1105 74-75 -0.10012187272658224 1105 76-77 -0.14951776624869595 1105 78-79 -0.5295126763444387 1105 80-81 -0.45574028505827613 1105 82-83 -0.5101411657466741 1105 84-85 -0.4158872517105934 1105 86-87 -0.4493307714506116 1105 88-89 -0.48776371373304883 1105 90-91 -0.44289963486807693 1105 92-93 -0.22133681288968177 1105 94-95 -0.5242485680924425 1105 96-97 -0.7319020101337159 1105 98-99 -0.6124668348305882 1105 100-101 -0.43484003196546794 1106 1 -0.04103381141500151 1106 2 -0.012166432559720164 1106 3 -0.02424907081955041 1106 4 0.1593528937834563 1106 5 0.1305094696961504 1106 6 0.08476533244603957 1106 7 0.24108648682069145 1106 8 0.12425849835728542 1106 9 0.32867389286644766 1106 10-11 0.3392593025878057 1106 12-13 0.2816380705465633 1106 14-15 0.07176618507972421 1106 16-17 0.07340062140335135 1106 18-19 0.24293191739639042 1106 20-21 0.2146970658601859 1106 22-23 0.15231519805638527 1106 24-25 0.09853347167610593 1106 26-27 0.20054587070097796 1106 28-29 0.18348231629260425 1106 30-31 0.19731728419696282 1106 32-33 0.3576834916552869 1106 34-35 0.13237684571380726 1106 36-37 0.31904566022624437 1106 38-39 0.29986840662657244 1106 40-41 0.2805867026690052 1106 42-43 0.21407032100931644 1106 44-45 -0.044447531521456085 1106 46-47 -0.01668828882296225 1106 48-49 -0.15395505451435554 1106 50-51 -0.36904252639977386 1106 52-53 -0.46957055008978443 1106 54-55 -0.12848878000675512 1106 56-57 -0.18924338942580476 1106 58-59 -0.232163894794148 1106 60-61 -0.057927587863325414 1106 62-63 -0.3280223373661997 1106 64-65 -0.31128252745364904 1106 66-67 -0.311513425467993 1106 68-69 -0.4192464572298036 1106 70-71 -0.35354013137951057 1106 72-73 -0.5174453204444305 1106 74-75 -0.6799298073235533 1106 76-77 -0.8866317455862642 1106 78-79 -0.7761505985290214 1106 80-81 -0.9755907764209368 1106 82-83 -0.8985976430544014 1106 84-85 -0.7901394752082744 1106 86-87 -0.8122205251626582 1106 88-89 -0.5979960027457558 1106 90-91 -0.6654407217454725 1106 92-93 -1.0081656805624064 1106 94-95 -0.5885830120080371 1106 96-97 -0.5135046083362482 1106 98-99 -0.4625055652204111 1106 100-101 -0.04576293515815877 1107 1 -0.10945361151019384 1107 2 -0.05766064166649443 1107 3 -0.10645656663961134 1107 4 -0.12440392531367195 1107 5 0.0723137387255477 1107 6 0.1053751763543147 1107 7 -0.10521075869121432 1107 8 0.03652555067669283 1107 9 -0.1633018451825876 1107 10-11 0.04996635295454155 1107 12-13 0.06295404950652994 1107 14-15 0.1414231860194377 1107 16-17 0.088695535912926 1107 18-19 0.14399551348616058 1107 20-21 0.1866930018613644 1107 22-23 0.22465864867209007 1107 24-25 0.16887390785424117 1107 26-27 0.3572676393337417 1107 28-29 0.19572160405545702 1107 30-31 0.01439614774179887 1107 32-33 -0.09804271930652675 1107 34-35 -0.277948213171463 1107 36-37 -0.2094984396529327 1107 38-39 0.0793873170355397 1107 40-41 -0.12188037649963945 1107 42-43 -0.09701933104891935 1107 44-45 -0.29228586247496935 1107 46-47 -0.3034290349877935 1107 48-49 -0.3068050404795315 1107 50-51 -0.306553967837921 1107 52-53 -0.2514418201809434 1107 54-55 -0.3834208026089314 1107 56-57 -0.3131267050630413 1107 58-59 -0.4210452577592747 1107 60-61 -0.5049567803113604 1107 62-63 -0.7035761029723204 1107 64-65 -0.6709265919406491 1107 66-67 -0.7777230448189769 1107 68-69 -0.641594789525783 1107 70-71 -0.6527657748459958 1107 72-73 -0.5451046157738837 1107 74-75 -0.43775727653845564 1107 76-77 -0.35457741248481156 1107 78-79 -0.3093872135719167 1107 80-81 -0.4382749072987835 1107 82-83 -0.3339962322488006 1107 84-85 -0.24534700764093742 1107 86-87 -0.19660555549010184 1107 88-89 -0.4524044651385104 1107 90-91 -0.19918991321717883 1107 92-93 -0.5738837757307635 1107 94-95 -0.5504723443162192 1107 96-97 -0.4459085917873544 1107 98-99 -0.5395201736486328 1107 100-101 -0.463926141933932 1108 1 0.14809537660416439 1108 2 0.08825969241638276 1108 3 0.20161875026962406 1108 4 0.049525502149322165 1108 5 0.14108242586879527 1108 6 0.043284440235311195 1108 7 0.15879996556686393 1108 8 0.19266358225642222 1108 9 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402.0 5 397.0 6 466.0 7 592.0 8 781.0 9 877.0 10 1154.0 11 1642.0 12 2411.0 13 3698.0 14 3416.0 15 3749.0 16 4935.0 17 6934.0 18 5643.0 19 3610.0 20 3431.0 21 3461.0 22 3975.0 23 4394.0 24 5134.0 25 5893.0 26 6932.0 27 8069.0 28 9675.0 29 11393.0 30 13650.0 31 16336.0 32 20506.0 33 25800.0 34 35058.0 35 54801.0 36 70841.0 37 114358.0 38 118588.0 39 18776.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64446267210796 22.341039584409273 16.107347436144106 18.90715030733866 2 13.4893603409001 24.48897815835153 40.21890473552799 21.802756765220376 3 19.688660262508733 29.381277520058596 33.656347609385655 17.273714608047012 4 10.650207036374077 19.351123250210065 42.80879066846059 27.18987904495527 5 10.05217315154128 41.19388607458504 36.89440073291658 11.859540040957103 6 23.429723620585477 41.28456425766687 19.408159933484477 15.877552188263175 7 22.08650602896326 37.80876976833431 24.176445638342994 15.928278564359436 8 21.243269536647887 33.960247556498864 22.718879970107402 22.077602936745848 9 23.23772140521088 21.082034596694513 25.731228423354203 29.949015574740407 10-11 21.333070762104576 27.49693035718944 30.193166583771585 20.976832296934397 12-13 26.3858651058476 27.01611172591972 28.606245239908606 17.99177792832407 14-15 22.76466415156582 28.621909316672966 30.47945407219961 18.133972459561605 16-17 16.929612620732545 31.584736427082465 32.31092002050045 19.174730931684543 18-19 16.556651452781548 32.85054567153326 33.32598308826438 17.26681978742081 20-21 18.472601667483072 30.38291848253317 31.249147826262607 19.89533202372115 22-23 17.83379492057613 29.688786376301636 31.209162156019293 21.268256547102943 24-25 19.596051947002337 30.39350881358044 31.773845664263956 18.23659357515326 26-27 20.057211069786394 28.175946769123534 34.08823435324384 17.678607807846234 28-29 17.424472011206294 29.649365605301437 34.77764196433382 18.14852041915845 30-31 17.920350443905154 30.92116948195212 32.894184156453946 18.26429591768878 32-33 17.874361167035882 30.94922666702578 32.52871802468471 18.64769414125363 34-35 19.26344533347735 28.94592500957759 32.24095360733399 19.549676049611072 36-37 20.068343234535483 28.659689750121103 33.29636057904067 17.975606436302733 38-39 19.990297408106397 28.790835614597498 33.64010314497515 17.578763832320956 40-41 17.67114154641501 29.76413296705061 34.92391972087702 17.640805765657362 42-43 17.05749594265621 31.19142209899919 32.262645388152556 19.488436570192047 44-45 18.04230209988841 30.671896662496195 31.34573766611436 19.94006357150103 46-47 19.03076103770953 30.462303147110287 30.237871833236124 20.269063981944058 48-49 19.081840309981832 29.82180102960908 31.273805165631114 19.822553494777974 50-51 19.056818162603797 30.50203495695901 32.260788991787464 18.180357888649727 52-53 17.96697922188377 31.377348074238835 32.25828541862701 18.397387285250385 54-55 17.01369721735911 30.041734963958472 32.77238678852115 20.17218103016127 56-57 19.1891540115485 31.481155931790827 29.937609027843514 19.392081028817156 58-59 19.154867451065225 29.66456314453462 31.037711295812446 20.14285810858771 60-61 18.57358841167279 29.663921618311583 32.21445162380168 19.548038346213943 62-63 20.147548078223473 29.182839252387005 31.856979118768763 18.81263355062076 64-65 19.378659327930738 30.352267779313724 32.080252628375035 18.1888202643805 66-67 18.20486305174803 29.979630886982285 31.298845919370784 20.516660141898903 68-69 17.3196697536006 30.99979619639902 32.0487504978812 19.631783552119177 70-71 19.059586789159642 30.687991313642453 30.260861329472732 19.991560567725173 72-73 18.581292467539495 29.002306649034214 31.148217420273483 21.268183463152806 74-75 18.320193728840504 31.418028822576442 30.576975760219977 19.68480168836308 76-77 19.677926557110975 29.04223304253736 31.31356935145736 19.9662710488943 78-79 18.573039253802488 29.043114610555353 32.58342827585508 19.800417859787082 80-81 18.85681200579835 30.902759288138316 30.924566497195915 19.315862208867422 82-83 18.46763115963162 30.359888588805628 31.06352044158731 20.108959809975442 84-85 18.578116203463416 29.29719001110741 30.752818695614685 21.371875089814488 86-87 19.133051563820793 29.87142857142857 31.18005071851226 19.815469146238378 88-89 20.30507660729925 28.308688021803746 31.25217682405175 20.134058546845253 90-91 18.941797152423145 28.17215665054618 32.583448882275356 20.302597314755317 92-93 19.15733206166251 30.329180110527133 31.536590975195654 18.976896852614704 94-95 18.814930315386086 29.880163772794845 30.97172872974614 20.333177182072934 96-97 18.46674546767383 28.90238313861368 30.512308007095474 22.11856338661702 98-99 19.54523701214697 29.151538621539856 29.868130232809836 21.435094133503345 100-101 20.417324292405976 28.191580643443288 31.04385176643839 20.34724329771235 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9525.0 1 5479.5 2 1293.0 3 1040.5 4 789.5 5 811.0 6 1335.5 7 2312.5 8 3194.0 9 3268.0 10 2477.0 11 1688.0 12 1503.0 13 1511.5 14 1456.5 15 1392.5 16 1393.5 17 1390.5 18 1335.0 19 1321.5 20 1437.5 21 1655.0 22 1925.5 23 2171.0 24 2359.5 25 3031.0 26 4612.5 27 6141.0 28 6394.5 29 6947.0 30 8728.0 31 10612.0 32 13129.0 33 16313.0 34 19916.0 35 22520.0 36 26270.0 37 30777.5 38 29583.0 39 25675.5 40 22865.5 41 20117.5 42 17429.0 43 16319.5 44 16627.5 45 16457.0 46 17602.5 47 24931.0 48 55812.0 49 54511.0 50 20509.0 51 9508.0 52 5181.5 53 3480.5 54 2607.0 55 2081.5 56 1648.5 57 1285.0 58 1141.0 59 1042.0 60 841.5 61 664.5 62 505.5 63 334.5 64 211.0 65 157.5 66 123.0 67 95.5 68 76.5 69 57.5 70 47.0 71 42.5 72 46.5 73 42.0 74 28.5 75 26.0 76 21.0 77 17.0 78 13.5 79 7.0 80 6.5 81 5.5 82 2.0 83 2.0 84 2.0 85 0.0 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3337314336658312 2 0.08965668051607259 3 0.10176790189910678 4 0.10647782132584233 5 0.11741156285219265 6 0.05769651297751014 7 0.060219684098975594 8 0.06106074113946408 9 0.06509781493380881 10-11 0.06148126965970833 12-13 0.06013557839492674 14-15 0.058117041497754374 16-17 0.05996736698682905 18-19 0.06820972598361622 20-21 0.07081700280913052 22-23 0.08831098925129102 24-25 0.09705798247237128 26-27 0.09234806304563575 28-29 0.09049773755656108 30-31 0.08418980975289744 32-33 0.07367659674679136 34-35 0.3298625712795842 36-37 0.5153156487072953 38-39 0.4870561321468822 40-41 0.4675436088075493 42-43 0.49958788205016064 44-45 0.5096805665360225 46-47 0.503793167252603 48-49 0.5215394708069101 50-51 0.5175865027166142 52-53 0.51716597419637 54-55 0.5079143467509967 56-57 0.48747666066712647 58-59 0.46830056014398896 60-61 0.4230516913657084 62-63 0.4968964995205975 64-65 0.522716950663594 66-67 0.530454675436088 68-69 0.5440797994920015 70-71 0.5413043112583895 72-73 0.5308752039563323 74-75 0.5295295126915508 76-77 0.5063163383740685 78-79 0.48848592911571265 80-81 0.49496206832747397 82-83 0.5025315816918704 84-85 0.5034567444364078 86-87 0.5029521102121146 88-89 0.5100169893522178 90-91 0.5231374791838382 92-93 0.5285202442429646 94-95 0.5354169119749701 96-97 0.5259970731214991 98-99 0.5296977240996484 100-101 0.509932883648169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 594490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.971351197434917 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7245432155755 18.152196196858977 2 10.426655002969607 4.998820177813525 3 3.801481008407824 2.73379908968769 4 1.9459347012604447 1.8658673652475886 5 1.2340459814003928 1.4790874806966026 6 0.9062751418495743 1.3034783824068774 7 0.6371354218862311 1.0691097870852495 8 0.4668517257758461 0.8952853340561109 9 0.37409793382068635 0.8070869658465396 >10 3.492070033904841 17.758961493816333 >50 0.5466846742373627 9.22801252792675 >100 0.39931139876901073 17.570485762315876 >500 0.01894799256021668 3.177275956885471 >1k 0.021755102569137666 11.516048229181544 >5k 0.004210665013381484 7.444485250174869 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9821 1.652004238927484 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8013 1.3478780130868475 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7054 1.186563272721156 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6821 1.1473700146343924 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6393 1.0753755319685783 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6151 1.0346683712089353 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 0.8008545139531362 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4408 0.7414758868946492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4091 0.6881528705276793 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3625 0.6097663543541523 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3327 0.5596393547410385 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3271 0.5502195158875676 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3086 0.5191004053894935 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2735 0.46005820114720186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2668 0.44878803680465607 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2500 0.420528520244243 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2363 0.3974835573348584 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2247 0.37797103399552556 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2153 0.36215916163434203 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1968 0.33104005113626805 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1911 0.32145200087469933 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1898 0.3192652525694293 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1865 0.31371427610220526 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1805 0.30362159161634344 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1770 0.297734192332924 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1661 0.27939914885027506 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1646 0.27687597772880956 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1535 0.25820451142996514 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1478 0.24861646116839642 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1438 0.24188800484448858 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1355 0.22792645797237968 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1209 0.20336759239011593 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1207 0.20303116957392048 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1145 0.19260206227186327 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1129 0.1899106797423001 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1126 0.18940604551800702 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1075 0.18082726370502447 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 999 0.1680431966895995 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 995 0.16737035105720868 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.16720213964911101 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 940 0.15811872361183535 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 932 0.15677303234705378 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 872 0.14668034786119194 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 803 0.13507376070245083 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 765 0.12868172719473833 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 711 0.1195983111574627 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 699 0.11757977426029033 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 689 0.11589766017931337 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 665 0.11186058638496864 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 664 0.11169237497687093 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 649 0.10916920385540548 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 624 0.10496391865296305 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 614 0.10328180457198606 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 612 0.10294538175579068 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 610 0.10260895893959528 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 608 0.10227253612339988 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.682114080976972E-4 0.0 14-15 0.0 0.0 0.0 2.523171121465458E-4 0.0 16-17 0.0 0.0 0.0 5.887399283419402E-4 0.0 18-19 0.0 0.0 0.0 9.251627445373346E-4 0.0 20-21 0.0 0.0 0.0 0.0020185368971723664 0.0 22-23 0.0 0.0 0.0 0.0037006509781493378 0.0 24-25 0.0 0.0 0.0 0.00479402513078437 0.0 26-27 0.0 0.0 0.0 0.008074147588689465 0.0 28-29 0.0 0.0 0.0 0.025315816918703427 0.0 30-31 0.0 0.0 0.0 0.06963952295244663 0.0 32-33 0.0 0.0 0.0 0.134989654998402 0.0 34-35 0.0 0.0 0.0 0.2386919880906323 0.0 36-37 0.0 0.0 0.0 0.3767935541388417 0.0 38-39 0.0 0.0 0.0 0.5551817524264495 0.0 40-41 0.0 0.0 0.0 0.7358408047233763 0.0 42-43 0.0 0.0 0.0 0.922134939191576 0.0 44-45 0.0 0.0 0.0 1.1400528183821428 0.0 46-47 0.0 0.0 0.0 1.4029672492388432 0.0 48-49 0.0 0.0 0.0 1.679927332671702 0.0 50-51 0.0 0.0 0.0 1.9450285118336725 0.0 52-53 0.0 0.0 0.0 2.1894396877996263 0.0 54-55 0.0 0.0 0.0 2.460848794765261 0.0 56-57 0.0 0.0 0.0 2.78961799189221 0.0 58-59 0.0 0.0 0.0 3.1560665444330436 0.0 60-61 0.0 0.0 0.0 3.6283200726673286 0.0 62-63 0.0 0.0 0.0 4.0476711130548875 0.0 64-65 0.0 0.0 0.0 4.429258692324513 0.0 66-67 0.0 0.0 0.0 4.877037460680583 0.0 68-69 0.0 0.0 0.0 5.3940352234688556 0.0 70-71 0.0 0.0 0.0 5.978990395128598 0.0 72-73 0.0 0.0 0.0 6.585056098504601 0.0 74-75 0.0 0.0 0.0 7.238557418964154 0.0 76-77 0.0 0.0 0.0 7.847482716277819 0.0 78-79 0.0 0.0 0.0 8.58206193544046 0.0 80-81 0.0 0.0 0.0 9.367020471328367 0.0 82-83 0.0 0.0 0.0 10.108412252518967 0.0 84-85 0.0 0.0 0.0 11.004474423455399 0.0 86-87 0.0 0.0 0.0 11.979091321973456 0.0 88-89 0.0 0.0 0.0 12.873219061716766 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 45 5.632155E-7 36.999443 94-95 CGCAGAT 55 0.0026263904 34.4164 8 ACATATG 80 4.5905772E-4 29.576593 8 ATGGGTC 65 0.005927664 29.143637 5 CTGTACC 65 0.005927664 29.143637 4 CATTCCG 180 2.2009772E-10 28.919336 9 TGGGAAC 215 1.8189894E-12 28.616043 6 GCATATG 85 6.423751E-4 27.92608 1 CTCGCGG 35 0.008330999 27.183264 94-95 GACATAT 175 4.978574E-9 27.128195 1 GCCTGTG 70 0.0085394 27.043732 7 GGCATTC 195 5.5661076E-10 26.69702 7 GGATGAC 180 6.9558155E-9 26.290304 8 CGCAGAA 180 6.9558155E-9 26.290304 9 CCCTATA 145 8.0846803E-7 26.12878 2 TAGGCAT 200 7.421477E-10 26.047127 5 TTAGGCA 220 8.0035534E-11 25.831861 4 CCTTTAG 190 1.1954398E-8 24.986496 1 GAGTACT 3055 0.0 23.782454 12-13 GGTAGAT 80 1.8391056E-6 23.780325 86-87 >>END_MODULE