##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938901_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594490 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11638042692056 33.0 31.0 34.0 30.0 34.0 2 32.361247455802456 34.0 31.0 34.0 30.0 34.0 3 32.420745512960686 34.0 31.0 34.0 30.0 34.0 4 35.958948005853756 37.0 35.0 37.0 35.0 37.0 5 35.899762821914585 37.0 35.0 37.0 35.0 37.0 6 35.969225722888524 37.0 35.0 37.0 35.0 37.0 7 35.92841763528403 37.0 35.0 37.0 35.0 37.0 8 35.928955911789934 37.0 35.0 37.0 35.0 37.0 9 37.54946761089337 39.0 37.0 39.0 35.0 39.0 10-11 37.47486669245909 39.0 37.0 39.0 34.5 39.0 12-13 37.41654359198641 39.0 37.0 39.0 34.0 39.0 14-15 38.564647008360105 40.0 38.0 41.0 34.0 41.0 16-17 38.650944507056465 40.0 38.0 41.0 34.0 41.0 18-19 38.74910006896668 40.0 38.0 41.0 34.0 41.0 20-21 38.73671466298845 40.0 38.0 41.0 34.0 41.0 22-23 38.67022153442446 40.0 38.0 41.0 34.0 41.0 24-25 38.558174233376505 40.0 38.0 41.0 34.0 41.0 26-27 38.29678295682013 40.0 37.5 41.0 34.0 41.0 28-29 38.12723679119918 40.0 37.0 41.0 33.5 41.0 30-31 37.96814748776262 40.0 37.0 41.0 33.0 41.0 32-33 37.827865901865465 40.0 37.0 41.0 33.0 41.0 34-35 37.70622802738482 40.0 36.0 41.0 33.0 41.0 36-37 37.515805143904856 40.0 36.0 41.0 33.0 41.0 38-39 37.30006560244916 40.0 36.0 41.0 32.5 41.0 40-41 37.143269020504974 40.0 35.5 41.0 32.0 41.0 42-43 37.00294874598396 40.0 35.0 41.0 31.0 41.0 44-45 36.76618866591532 40.0 35.0 41.0 31.0 41.0 46-47 36.40416827869266 40.0 35.0 41.0 30.0 41.0 48-49 36.02814765597403 39.0 35.0 41.0 29.5 41.0 50-51 35.80073171962522 39.0 35.0 41.0 28.0 41.0 52-53 35.52219970058369 39.0 35.0 40.0 27.0 41.0 54-55 35.30297061346701 39.0 35.0 40.0 27.0 41.0 56-57 35.275538697034435 39.0 35.0 40.0 27.0 41.0 58-59 35.093533112415685 38.5 35.0 40.0 26.5 41.0 60-61 34.73179868458679 38.0 34.0 40.0 26.0 41.0 62-63 34.30106898349846 38.0 34.0 40.0 25.5 41.0 64-65 33.83284832377332 37.0 33.0 39.5 23.0 41.0 66-67 33.369884270551225 36.5 33.0 39.0 22.0 40.5 68-69 32.89166007838652 36.0 32.5 39.0 21.5 40.0 70-71 32.319170213123854 35.5 32.0 38.0 20.5 40.0 72-73 31.752488687782805 35.0 31.0 37.0 18.5 39.5 74-75 31.213149926828038 35.0 31.0 37.0 15.0 39.0 76-77 29.198812427458833 32.5 28.5 35.0 9.0 37.0 78-79 30.13786186479167 34.0 29.5 36.0 7.5 38.0 80-81 30.13239835825666 34.0 30.0 35.5 6.5 37.0 82-83 29.71648303587949 34.0 29.5 35.0 2.0 37.0 84-85 29.274791838382477 34.0 29.0 35.0 2.0 36.0 86-87 28.607323083651533 34.0 29.0 35.0 2.0 36.0 88-89 27.833611162509044 33.0 26.5 35.0 2.0 35.0 90-91 27.19638429578294 33.0 26.0 35.0 2.0 35.0 92-93 26.85664350956282 33.0 25.0 35.0 2.0 35.0 94-95 26.356035425322546 32.0 25.0 34.0 2.0 35.0 96-97 25.453677942438055 32.0 22.0 34.0 2.0 35.0 98-99 24.299159784016553 32.0 10.0 34.0 2.0 35.0 100-101 18.763402243940185 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19839834025935232 1101 2 0.23545630161692088 1101 3 0.30595421553402247 1101 4 0.23107908415766332 1101 5 0.26558750637878603 1101 6 0.25589621188338185 1101 7 0.17446969570614357 1101 8 0.21395740949230913 1101 9 0.33400837845614717 1101 10-11 0.42226342180064336 1101 12-13 0.3227400688536619 1101 14-15 0.49561217464175655 1101 16-17 0.45216372476512845 1101 18-19 0.3895161452613394 1101 20-21 0.4655013811977611 1101 22-23 0.48164103457752816 1101 24-25 0.4830898437565949 1101 26-27 0.5287732046914897 1101 28-29 0.7180474968660562 1101 30-31 0.7266354837805764 1101 32-33 0.7850918724452711 1101 34-35 0.9360263106293729 1101 36-37 0.8520235292537706 1101 38-39 0.9993795879550476 1101 40-41 1.0032351121930247 1101 42-43 0.8564225392757479 1101 44-45 1.071778248056944 1101 46-47 1.1538435322334806 1101 48-49 0.9926202065976568 1101 50-51 0.7418649511195667 1101 52-53 0.5583780387267225 1101 54-55 0.3576914290633866 1101 56-57 0.6043761582601164 1101 58-59 0.5929032379544097 1101 60-61 0.8160011061454497 1101 62-63 0.5952228199133742 1101 64-65 0.409454894550457 1101 66-67 0.544790645032954 1101 68-69 0.9599859845408432 1101 70-71 0.7890434468336451 1101 72-73 1.1112462106229373 1101 74-75 1.1663706378938095 1101 76-77 0.7618057117156454 1101 78-79 1.0598025310948174 1101 80-81 1.0307543549499094 1101 82-83 0.9768828040375368 1101 84-85 1.1398330563730177 1101 86-87 0.9872949844861694 1101 88-89 1.0793759679416866 1101 90-91 1.1385420539754953 1101 92-93 1.041286466190499 1101 94-95 0.7982653393476689 1101 96-97 1.2987565520946305 1101 98-99 2.0156950785381405 1101 100-101 1.9727697576614638 1102 1 0.17915447578285892 1102 2 0.1896135878116496 1102 3 0.17235197533835134 1102 4 0.13943882178239164 1102 5 0.034331079018379285 1102 6 -0.03693085390499817 1102 7 0.22455446737851048 1102 8 0.09635061108212284 1102 9 0.3174153658654717 1102 10-11 0.29577296903653405 1102 12-13 0.18629936733333352 1102 14-15 0.39488675147824637 1102 16-17 0.2693956517225189 1102 18-19 0.17501775731281555 1102 20-21 0.2080908083636146 1102 22-23 0.3090281909743169 1102 24-25 0.3320920394818785 1102 26-27 0.3027924242362232 1102 28-29 0.23129828204628922 1102 30-31 0.18248472917372993 1102 32-33 0.05669822615160314 1102 34-35 0.007390856614534869 1102 36-37 0.030983825537710175 1102 38-39 -0.02637675602338163 1102 40-41 0.0010168042911899988 1102 42-43 0.06608303567642793 1102 44-45 -0.23060300211676577 1102 46-47 -0.2783634496170393 1102 48-49 -0.19649358200124567 1102 50-51 -0.3476803391886705 1102 52-53 -0.17557961712808634 1102 54-55 -0.22372759314401236 1102 56-57 -0.23258602801520567 1102 58-59 -0.31538527756853085 1102 60-61 -0.1274624420354371 1102 62-63 -0.23617535680082113 1102 64-65 -0.06693411014672535 1102 66-67 -0.1302281159109242 1102 68-69 -0.4067105606660917 1102 70-71 -0.22991552923573266 1102 72-73 -0.3225293945912142 1102 74-75 -0.21997452178267096 1102 76-77 -0.4114290224348309 1102 78-79 -0.3140978691386529 1102 80-81 -0.14289555194022086 1102 82-83 -0.2116924759869221 1102 84-85 -0.022181591060203232 1102 86-87 0.08562665018004623 1102 88-89 -0.22323284553283784 1102 90-91 -0.02985305150270179 1102 92-93 0.0049927253972583685 1102 94-95 0.2316848340529134 1102 96-97 0.08213539808812698 1102 98-99 0.4786595856117124 1102 100-101 0.8735156788932947 1103 1 0.06262493456057427 1103 2 0.06519171545681246 1103 3 -2.6695135878895826E-4 1103 4 0.0020763704534587646 1103 5 0.07724965020511121 1103 6 0.004125519888809492 1103 7 0.1045375383112983 1103 8 0.13547029958728984 1103 9 -0.007348473646970888 1103 10-11 0.0668902874722832 1103 12-13 0.10408335243575095 1103 14-15 0.07151448129033611 1103 16-17 0.10934486452089942 1103 18-19 -0.13066363764232136 1103 20-21 0.22784195107564642 1103 22-23 0.12399856510670304 1103 24-25 0.11454167550693484 1103 26-27 0.22163955475933506 1103 28-29 0.11190095683891599 1103 30-31 0.04586207808180376 1103 32-33 -0.1250234599834954 1103 34-35 -0.27484885897713696 1103 36-37 -0.1962973662686167 1103 38-39 -0.10450440119013393 1103 40-41 -0.01708714018146651 1103 42-43 -0.04043634810552987 1103 44-45 -0.05877127704481211 1103 46-47 -0.0731239033160449 1103 48-49 -0.13330245283247422 1103 50-51 0.07149860105172223 1103 52-53 0.08948048106051232 1103 54-55 0.1538583218720717 1103 56-57 0.21750031022755678 1103 58-59 -0.013484143321029762 1103 60-61 -0.08574244880706772 1103 62-63 -0.054688984699922116 1103 64-65 -0.08416790056487145 1103 66-67 -0.221193072741805 1103 68-69 -0.01969515521492582 1103 70-71 -0.036472156965537295 1103 72-73 0.06647348335021164 1103 74-75 -0.01734374473848277 1103 76-77 -0.41888289072668883 1103 78-79 -0.07380330336922825 1103 80-81 -0.02924564505008931 1103 82-83 0.08468470363047942 1103 84-85 0.22160374836759544 1103 86-87 0.24139946209811214 1103 88-89 0.2048067684844277 1103 90-91 -0.20144776963373445 1103 92-93 0.16791129658398773 1103 94-95 0.5699885415186294 1103 96-97 0.5231968506020905 1103 98-99 0.9849081043183325 1103 100-101 0.4924203682449111 1104 1 0.06186009054849961 1104 2 0.10684887748298166 1104 3 -0.012249507547984706 1104 4 0.11250911114449735 1104 5 0.020000404985381692 1104 6 0.1073928798351318 1104 7 0.1843832276772872 1104 8 0.24545352669228748 1104 9 0.194778329439238 1104 10-11 0.11589397750918806 1104 12-13 0.13065161811840653 1104 14-15 0.2769086443228801 1104 16-17 0.15810702486977846 1104 18-19 0.27214749955996353 1104 20-21 0.31997210874085624 1104 22-23 0.13817501596212622 1104 24-25 0.3151656272009973 1104 26-27 0.05255535482642415 1104 28-29 0.21748297629547153 1104 30-31 0.2907429905272849 1104 32-33 0.21611709687662284 1104 34-35 0.38240374082158723 1104 36-37 0.3180937776428223 1104 38-39 0.47265091581758156 1104 40-41 0.11550930396479231 1104 42-43 0.03636002894914725 1104 44-45 0.2650026243305632 1104 46-47 0.3169968615279686 1104 48-49 0.2982943267716891 1104 50-51 0.14958649102153032 1104 52-53 0.07994341296255669 1104 54-55 0.5544956918821384 1104 56-57 0.7719182907709978 1104 58-59 0.6408633910634123 1104 60-61 0.4805037772915597 1104 62-63 0.383479415165894 1104 64-65 0.1789652762214402 1104 66-67 0.6261366823739323 1104 68-69 0.6804725737350878 1104 70-71 0.5065972828197687 1104 72-73 0.5095395685565194 1104 74-75 0.6445918473447101 1104 76-77 0.4266353917288619 1104 78-79 0.7715975688213135 1104 80-81 0.9560311077174362 1104 82-83 0.6990423017519163 1104 84-85 0.8169341743991261 1104 86-87 1.43162086431213 1104 88-89 1.2029818775082468 1104 90-91 1.3911822203025288 1104 92-93 1.165938804131791 1104 94-95 1.0602166528906558 1104 96-97 1.1733813524471124 1104 98-99 1.151620616898004 1104 100-101 1.3320010458698697 1105 1 0.019523330282503082 1105 2 0.027901162872140617 1105 3 0.08739793277668184 1105 4 0.10760221715577245 1105 5 0.059495639509918874 1105 6 -0.0082808972234929 1105 7 -0.04408991266909368 1105 8 -0.12092898740023372 1105 9 -0.054157742809181286 1105 10-11 0.14929670458458588 1105 12-13 0.013976400564096991 1105 14-15 -0.027986409975262916 1105 16-17 -0.00534319252010107 1105 18-19 0.1183203195769309 1105 20-21 0.14422341275122363 1105 22-23 0.10850836902827155 1105 24-25 0.08889105162101174 1105 26-27 -0.059982548627473875 1105 28-29 0.07614338108134433 1105 30-31 0.037288103037234066 1105 32-33 0.11363964162078588 1105 34-35 0.21155042801038348 1105 36-37 0.3725974643909211 1105 38-39 0.01581645442483648 1105 40-41 0.09899129119108352 1105 42-43 0.14910019378395134 1105 44-45 0.1761128584275582 1105 46-47 -0.19353388549073003 1105 48-49 -0.10515628527453913 1105 50-51 -0.1469862474331194 1105 52-53 -0.30326461697870855 1105 54-55 -0.4739491647589418 1105 56-57 -0.1589346273838501 1105 58-59 -0.6731811130179963 1105 60-61 -0.31946793900313963 1105 62-63 -0.49215779040402197 1105 64-65 -0.6565386670532902 1105 66-67 -0.4696957465385978 1105 68-69 -0.22967732990298373 1105 70-71 -0.14326360081580702 1105 72-73 -0.31110227422554715 1105 74-75 -0.4631904470557764 1105 76-77 -0.7082055288728561 1105 78-79 -0.4698104334940041 1105 80-81 -0.3306538446935825 1105 82-83 -0.45292670456916184 1105 84-85 -0.2568632748053119 1105 86-87 -0.16314599244734396 1105 88-89 -0.21923957732662203 1105 90-91 -0.4357971457478165 1105 92-93 -0.3085236410274206 1105 94-95 -0.04637509484500413 1105 96-97 -0.07760528843533976 1105 98-99 -0.055074070369727224 1105 100-101 -0.05483453371587288 1106 1 -0.21583611573293382 1106 2 -0.10808450221730936 1106 3 -0.06497582015142456 1106 4 -0.08096345185337839 1106 5 0.03551079569138693 1106 6 0.08106031284454218 1106 7 0.017636062823434884 1106 8 0.08179427229869418 1106 9 -0.23961564800838886 1106 10-11 0.09025852481957486 1106 12-13 0.1553049929244139 1106 14-15 0.01338977876772418 1106 16-17 -0.23617631586939325 1106 18-19 -0.05877088815795162 1106 20-21 0.1400377308630496 1106 22-23 0.14281976136245333 1106 24-25 0.1267628400317662 1106 26-27 0.033987642971297305 1106 28-29 -0.08861943027833519 1106 30-31 -0.08790438233387476 1106 32-33 0.028628769113765884 1106 34-35 -0.20369068033204485 1106 36-37 -0.2888802633017775 1106 38-39 -0.06388882130754325 1106 40-41 -0.08851979129998 1106 42-43 -0.1579851343977765 1106 44-45 0.09095266393566703 1106 46-47 -0.011113749421070906 1106 48-49 0.09383859111485293 1106 50-51 -0.0045144086262070005 1106 52-53 -0.23307861778131667 1106 54-55 -0.5990529902015425 1106 56-57 -0.518837941398651 1106 58-59 -0.6850802086867276 1106 60-61 -0.6659836038126201 1106 62-63 -0.6020727701988946 1106 64-65 -0.6522783241726771 1106 66-67 -0.5982246450089761 1106 68-69 -0.6462856676693107 1106 70-71 -0.6859930200466167 1106 72-73 -0.46848764627060646 1106 74-75 -0.23029948010259815 1106 76-77 -0.29753274000481156 1106 78-79 -0.08160593703573227 1106 80-81 -0.14594721573071823 1106 82-83 -0.25662895653134754 1106 84-85 -0.334982004448527 1106 86-87 -0.10127069496995134 1106 88-89 -0.30550102145686964 1106 90-91 0.022761337140817517 1106 92-93 0.42240280856240986 1106 94-95 0.35867012171853574 1106 96-97 0.3204917895200694 1106 98-99 0.453115307237578 1106 100-101 1.1087570333250305 1107 1 0.03926829120393194 1107 2 0.09344346370856016 1107 3 0.01959318850135361 1107 4 0.004523922901007893 1107 5 -0.041491608536148306 1107 6 -0.08664371088042344 1107 7 -0.012175748401986652 1107 8 0.10106470518169175 1107 9 0.003980197681698883 1107 10-11 0.006505672087669723 1107 12-13 0.19132111467351365 1107 14-15 0.01794804772389824 1107 16-17 -0.0037320585560252084 1107 18-19 0.0238118868332009 1107 20-21 -0.014850356616662452 1107 22-23 -0.11967276356462975 1107 24-25 -0.020563219597967475 1107 26-27 0.02412207224185181 1107 28-29 0.1597680897060485 1107 30-31 -0.1020749848552569 1107 32-33 -0.05110737606740656 1107 34-35 0.03588540675713148 1107 36-37 0.14513620016494855 1107 38-39 -0.04768621937195405 1107 40-41 -0.3025067206010448 1107 42-43 -0.13631047397965546 1107 44-45 -0.012372675646211917 1107 46-47 -0.22976725995940228 1107 48-49 -0.35431643884646036 1107 50-51 -0.501298601745475 1107 52-53 -0.2770579804779558 1107 54-55 -0.37044237742862407 1107 56-57 -0.5523598296325858 1107 58-59 -0.6641484789853607 1107 60-61 -0.4567913998560158 1107 62-63 -0.5176959777069214 1107 64-65 -0.5547972711942393 1107 66-67 -0.4787455202942468 1107 68-69 -0.8231916587114299 1107 70-71 -0.5300385905319729 1107 72-73 -0.5360439991672727 1107 74-75 -0.931224863619601 1107 76-77 -0.941819953663753 1107 78-79 -0.682754352320277 1107 80-81 -0.7987910995955438 1107 82-83 -0.9244062054604285 1107 84-85 -0.8996200278561837 1107 86-87 -0.773285852587204 1107 88-89 -0.6636897350120741 1107 90-91 -0.24767154585751072 1107 92-93 -0.06995583628314606 1107 94-95 -0.4589625074324175 1107 96-97 -0.06176818436293985 1107 98-99 0.04109691550714345 1107 100-101 -0.34852368769914577 1108 1 0.0746904662020782 1108 2 0.12145255708868063 1108 3 0.056250877102506536 1108 4 0.08562921337025386 1108 5 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-1.0014407766185585 2316 58-59 -0.9352642347379927 2316 60-61 -0.9761589038603944 2316 62-63 -1.1009332711397377 2316 64-65 -1.1314126948767083 2316 66-67 -0.867658283508689 2316 68-69 -0.720025272025488 2316 70-71 -0.6698596633139431 2316 72-73 -0.6223431748519239 2316 74-75 -0.7004214491569698 2316 76-77 -0.445414124855688 2316 78-79 -0.9300196568481489 2316 80-81 -1.159189779128301 2316 82-83 -0.9060593288733294 2316 84-85 -1.2307476990676527 2316 86-87 -1.3922597557789835 2316 88-89 -1.4865010019574179 2316 90-91 -1.783344671614433 2316 92-93 -2.000826641455628 2316 94-95 -1.9945721491873094 2316 96-97 -1.7307137639230454 2316 98-99 -1.3779512151331694 2316 100-101 -1.0969219374940415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 50.0 6 112.0 7 176.0 8 243.0 9 402.0 10 736.0 11 1065.0 12 1535.0 13 2112.0 14 2591.0 15 3493.0 16 4841.0 17 6754.0 18 6613.0 19 4061.0 20 3696.0 21 4004.0 22 4242.0 23 4869.0 24 5491.0 25 6517.0 26 7520.0 27 8877.0 28 10704.0 29 12910.0 30 15811.0 31 18712.0 32 23228.0 33 29119.0 34 40764.0 35 57517.0 36 81838.0 37 129348.0 38 89870.0 39 4660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61837996497349 22.992857902834377 16.513123118000824 18.875639014191307 2 13.641945196723244 24.943565072583223 39.79394102508032 21.620548705613217 3 19.571901966391362 29.48005853757002 33.617722753957175 17.330316742081447 4 10.806741913236555 19.982506013557842 41.99296876314151 27.217783310064085 5 9.816481353765411 41.705663678110646 36.72576494137832 11.752090026745615 6 23.48786354690575 41.79599320425911 18.760786556544264 15.95535669229087 7 22.09860552742687 37.852781375632894 23.663476256959747 16.38513683998049 8 21.555450890679406 34.37770189574257 22.29793604602264 21.768911167555384 9 23.08617470436845 20.985046005820116 25.358879039176436 30.569900250634994 10-11 21.490353075745595 27.877676664031352 29.738935894632373 20.893034365590673 12-13 26.291190768557925 27.077663207118707 28.559269289643225 18.071876734680146 14-15 22.710432315729008 28.837084902970556 30.12164320516347 18.330839576136974 16-17 16.957048692502887 31.921668765155164 31.869858387772076 19.251424154569868 18-19 16.614016407368347 32.965117941627184 32.948128560831236 17.47273709017324 20-21 18.536644855254085 30.44189136907265 31.0736934178876 19.947770357785664 22-23 17.91661760500597 29.788390048613095 30.96124409157429 21.333748254806643 24-25 19.67186917829361 30.648868733373313 31.53845700968143 18.14080507865165 26-27 20.04398728321755 28.352789786203303 34.0175612710054 17.585661659573752 28-29 17.394994028495013 29.921613483826476 34.520933909737764 18.162458577940757 30-31 17.810980840720617 31.19287120052482 32.706773873404096 18.289374085350467 32-33 17.719894363235714 31.08538411075039 32.53561876566469 18.659102760349207 34-35 19.19456414492361 28.995741251817936 32.19842418746903 19.611270415789416 36-37 19.872298542117083 28.844041019256167 33.256723675244594 18.026936763382153 38-39 19.829146675459434 29.01823265391267 33.55536751297045 17.597253157657445 40-41 17.632601771191766 29.85767719697843 34.75835571364528 17.751365318184533 42-43 17.0419085524534 31.210541986774576 32.36814526100114 19.379404199770885 44-45 17.988058481312674 30.772413897512195 31.236937124094062 20.002590497081066 46-47 19.06078370354172 30.53685122416882 30.1128708041414 20.28949426814806 48-49 19.09879691558223 29.863559595742107 31.223169468032136 19.81447402064353 50-51 18.96993282426569 30.673834769729456 32.09102661040513 18.265205795599723 52-53 17.937559925314133 31.6432572457064 32.02778852461774 18.39139430436172 54-55 16.93872058403001 30.096973876768324 32.51156453430672 20.452741004894953 56-57 19.10309677202308 31.651163181886993 29.716647883059427 19.529092163030498 58-59 19.043710102021077 29.939695703003437 30.76713457866893 20.24945961630655 60-61 18.352379342334455 30.153924049781196 31.83799664077015 19.6556999671142 62-63 20.008225669302863 29.612073062110532 31.44072850160771 18.9389727669789 64-65 19.15388950099666 30.72154890367293 31.68801567743509 18.436545917895323 66-67 18.005520828427052 30.261019788856068 30.919078314347427 20.814381068369457 68-69 17.33072822483107 31.15810137718618 31.584950031372326 19.926220366610426 70-71 18.890325228620522 30.827541949857817 30.003473568498684 20.278659253022973 72-73 18.318895187471615 28.975676630389074 30.865027166142404 21.840401015996903 74-75 18.02856229709499 31.571010445928444 30.066022977678347 20.33440427929822 76-77 19.22313750415665 29.065837160259463 31.087464379069836 20.623560956514055 78-79 18.08794549098736 28.883098488486812 32.241885846311966 20.78707017421386 80-81 18.28107114291073 30.830725340272657 30.554268263450897 20.33393525336572 82-83 17.88415280324312 30.30732224259449 30.78226715335834 21.026257800804053 84-85 17.784334290176698 29.11910134661756 30.50516451510077 22.59139984810497 86-87 18.108388185170938 29.71116182631996 31.014305112311924 21.16614487619718 88-89 19.295586901099345 28.01365778167485 31.09696450564879 21.593790811577016 90-91 18.034216756787792 27.784523258680842 32.387962159259395 21.793297825271967 92-93 18.0277212400545 29.81227606856297 31.444263149926826 20.7157395414557 94-95 17.625141719064874 29.33172522145181 30.788950454678492 22.25418260480482 96-97 17.034685192349745 28.485172164376188 30.503793167252603 23.976349476021465 98-99 17.89197463371966 28.492068832108192 29.943985601103467 23.671970933068682 100-101 18.29333526216721 27.56452201646125 31.50363254368893 22.63851017768261 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8137.0 1 4881.0 2 1394.5 3 1036.0 4 787.0 5 798.0 6 1286.0 7 2261.0 8 3075.5 9 3129.0 10 2359.5 11 1547.0 12 1347.5 13 1305.5 14 1233.0 15 1143.5 16 1089.0 17 1108.5 18 1124.0 19 1219.5 20 1424.5 21 1571.5 22 1767.5 23 2012.0 24 2238.5 25 2925.5 26 4457.5 27 5967.5 28 6217.0 29 6773.5 30 8641.5 31 10426.5 32 12956.0 33 16436.5 34 20098.0 35 22686.0 36 26426.0 37 30732.5 38 29614.0 39 25877.5 40 23054.0 41 20037.0 42 17304.5 43 16734.0 44 16995.0 45 16775.0 46 17671.0 47 24859.5 48 54127.5 49 52391.0 50 21837.0 51 13299.0 52 8125.0 53 4270.0 54 2567.0 55 1915.0 56 1457.0 57 1160.5 58 1077.5 59 970.5 60 775.5 61 579.5 62 382.0 63 221.0 64 127.5 65 93.0 66 62.0 67 42.0 68 27.5 69 17.0 70 20.0 71 21.0 72 12.5 73 9.0 74 9.0 75 6.5 76 3.0 77 2.5 78 2.5 79 1.0 80 1.0 81 2.5 82 2.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39815640296724925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.251627445373346E-4 16-17 0.0024390654174166093 18-19 1.682114080976972E-4 20-21 0.0 22-23 0.0 24-25 2.523171121465458E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011186058638496863 36-37 0.02270854009318912 38-39 0.010429107302057225 40-41 0.005298659355077461 42-43 0.004878130834833219 44-45 0.0015980083769281232 46-47 8.410570404884859E-4 48-49 0.003027805345758549 50-51 7.569513364396373E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.410570404884859E-4 60-61 0.0014297969688304261 62-63 0.0015980083769281232 64-65 8.410570404884859E-4 66-67 0.0023549597133677605 68-69 0.0028595939376608523 70-71 8.41057040488486E-5 72-73 0.0 74-75 0.0 76-77 0.09461891705495466 78-79 0.0032801224579050953 80-81 5.887399283419401E-4 82-83 0.0 84-85 8.41057040488486E-5 86-87 0.09108647748490302 88-89 0.4361721811973288 90-91 0.3909233124190482 92-93 0.0 94-95 6.728456323907888E-4 96-97 0.0 98-99 0.0 100-101 5.046342242930916E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 594490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.46291381076181 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62965254497988 16.539365390342216 2 11.409045121408667 5.125607944505908 3 4.092617513647303 2.757963434084209 4 2.138977211585414 1.9219064298810713 5 1.3187760901813634 1.4811776824718705 6 0.9507533330250932 1.2814014105022313 7 0.6939851531121741 1.091225007621497 8 0.5224170800611 0.9388007874145873 9 0.43181993454732653 0.8729940574354863 >10 3.7328220039953592 17.9097388009952 >50 0.5949455899429722 9.363353220758333 >100 0.4340483549269482 18.03849157462646 >500 0.020954058513714742 3.189759916766416 >1k 0.024695854676878088 12.089652368590626 >5k 0.004490155395796017 7.398561974003874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9912 1.6673114770643747 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8329 1.4010328180457199 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7456 1.2541842587764302 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6646 1.1179330182172955 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6004 1.009941294218574 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5665 0.9529176268734545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4596 0.7730996316170162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4200 0.7064879140103282 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4160 0.6997594576864203 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3696 0.6217093643290889 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3265 0.5492102474389814 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3199 0.5381082945045333 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2937 0.4940369055829366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2830 0.47603828491648303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2705 0.4550118589042709 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2571 0.4324715302191795 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2474 0.4161550236337028 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2356 0.3963060774781746 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2146 0.36098168177765816 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2117 0.3561035509428249 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2023 0.3402916785816414 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1971 0.33154468536056114 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1952 0.3283486686067049 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1948 0.3276758229743141 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1861 0.3130414304698145 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1777 0.2989116721896079 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1725 0.2901646789685276 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1683 0.28309979982842437 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1538 0.25870914565425823 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1521 0.2558495517165974 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1445 0.24306548470117242 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1326 0.22304832713754646 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1246 0.20959141448973068 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1132 0.1904153139665932 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1131 0.1902471025584955 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1129 0.1899106797423001 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1108 0.18637824017224847 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1096 0.18435970327507611 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1054 0.17729482413497283 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 975 0.16400612289525476 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 949 0.15963262628471464 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 949 0.15963262628471464 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.15441807263368604 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 815 0.13709229759962321 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 761 0.12800888156234758 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 756 0.12716782452185907 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 732 0.12313075072751434 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 701 0.11791619707648573 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 700 0.11774798566838802 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 694 0.11673871721980184 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 661 0.11118774075257784 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 654 0.11001026089589397 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 652 0.10967383807969856 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.1079917239987216 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 641 0.1078235125906239 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 638 0.10731887836633079 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 631 0.10614139850964692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.682114080976972E-4 0.0 14-15 0.0 0.0 0.0 1.682114080976972E-4 0.0 16-17 0.0 0.0 0.0 4.2052852024424296E-4 0.0 18-19 0.0 0.0 0.0 7.569513364396374E-4 0.0 20-21 0.0 0.0 0.0 0.0020185368971723664 0.0 22-23 0.0 0.0 0.0 0.003868862386247035 0.0 24-25 0.0 0.0 0.0 0.0051304479469797635 0.0 26-27 0.0 0.0 0.0 0.008578781812982557 0.0 28-29 0.0 0.0 0.0 0.025988662551094215 0.0 30-31 0.0 0.0 0.0 0.07048057999293511 0.0 32-33 0.0 0.0 0.0 0.13675587478342782 0.0 34-35 0.0 0.0 0.0 0.2418038991404397 0.0 36-37 0.0 0.0 0.0 0.3813352621574795 0.0 38-39 0.0 0.0 0.0 0.5619943144544063 0.0 40-41 0.0 0.0 0.0 0.7436626351999193 0.0 42-43 0.0 0.0 0.0 0.9312183552288515 0.0 44-45 0.0 0.0 0.0 1.149809080051809 0.0 46-47 0.0 0.0 0.0 1.4150784706218777 0.0 48-49 0.0 0.0 0.0 1.6948981479923968 0.0 50-51 0.0 0.0 0.0 1.963111238204175 0.0 52-53 0.0 0.0 0.0 2.2096250567713502 0.0 54-55 0.0 0.0 0.0 2.484987131827281 0.0 56-57 0.0 0.0 0.0 2.8158589715554507 0.0 58-59 0.0 0.0 0.0 3.1860081750744333 0.0 60-61 0.0 0.0 0.0 3.664233208296187 0.0 62-63 0.0 0.0 0.0 4.0864438426214065 0.0 64-65 0.0 0.0 0.0 4.47181617857323 0.0 66-67 0.0 0.0 0.0 4.924473077764134 0.0 68-69 0.0 0.0 0.0 5.445255597234604 0.0 70-71 0.0 0.0 0.0 6.030883614526737 0.0 72-73 0.0 0.0 0.0 6.638547326279668 0.0 74-75 0.0 0.0 0.0 7.293814866524247 0.0 76-77 0.0 0.0 0.0 7.907113660448452 0.0 78-79 0.0 0.0 0.0 8.641945196723242 0.0 80-81 0.0 0.0 0.0 9.426819626907097 0.0 82-83 0.0 0.0 0.0 10.16728624535316 0.0 84-85 0.0 0.0 0.0 11.06208683072886 0.0 86-87 0.0 0.0 0.0 12.033928241013307 0.0 88-89 0.0 0.0 0.0 12.926962606603979 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGCG 25 0.005039043 56.945747 5 GCTTATG 85 3.55898E-7 39.225582 1 CTCCCGC 85 3.6640085E-7 39.080414 7 CGCAGAA 100 3.0520823E-8 37.963833 9 GTAAGCG 80 1.8189894E-12 35.591095 94-95 TACTCCC 95 8.7555054E-7 34.966686 5 GTACCCA 75 3.0273345E-4 31.754047 1 ACTCCCG 105 1.9138006E-6 31.636526 6 CTAGGTA 95 3.5107834E-5 29.971445 4 CGTGCAA 80 4.516173E-4 29.659245 9 CTGACCG 80 4.516173E-4 29.659245 4 TGACCGT 80 4.516173E-4 29.659245 5 CTTATGT 80 4.516173E-4 29.659245 2 ACCGTGC 80 4.516173E-4 29.659245 7 GGGATCC 80 4.516173E-4 29.659245 7 CTGTAGG 65 0.005763682 29.311426 1 AGGCGAG 65 0.005869077 29.202948 7 GCTTTGC 475 0.0 29.08002 1 CTTTGCG 480 0.0 28.670603 2 CATATAG 170 3.6707206E-9 27.914581 3 >>END_MODULE