##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938900_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 598077 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89624914517696 33.0 31.0 34.0 30.0 34.0 2 31.951165150975545 33.0 31.0 34.0 30.0 34.0 3 31.97012592024104 34.0 31.0 34.0 30.0 34.0 4 35.49324083688221 37.0 35.0 37.0 33.0 37.0 5 35.54938076535296 37.0 35.0 37.0 33.0 37.0 6 35.66441277628132 37.0 35.0 37.0 33.0 37.0 7 35.61249805627035 37.0 35.0 37.0 33.0 37.0 8 35.61739876303553 37.0 35.0 37.0 33.0 37.0 9 37.19960807722082 39.0 37.0 39.0 34.0 39.0 10-11 37.117598570083786 39.0 37.0 39.0 33.5 39.0 12-13 37.03903427150685 39.0 37.0 39.0 33.0 39.0 14-15 38.15466988364375 40.0 38.0 41.0 33.0 41.0 16-17 38.0906572230666 40.0 38.0 41.0 33.0 41.0 18-19 38.11023413373194 40.0 38.0 41.0 33.0 41.0 20-21 38.003293890251584 40.0 38.0 41.0 33.0 41.0 22-23 38.07823490955178 40.0 38.0 41.0 33.0 41.0 24-25 38.00207331163044 40.0 38.0 41.0 33.0 41.0 26-27 37.8453092160374 40.0 37.5 41.0 33.0 41.0 28-29 37.72402800977132 40.0 37.0 41.0 33.0 41.0 30-31 37.58370995707911 40.0 37.0 41.0 33.0 41.0 32-33 37.38718843894682 40.0 36.0 41.0 33.0 41.0 34-35 37.079080954459045 40.0 36.0 41.0 31.5 41.0 36-37 36.763426782839 39.0 35.0 41.0 31.0 41.0 38-39 36.50581446870554 39.0 35.0 41.0 30.0 41.0 40-41 36.39851473974087 39.0 35.0 41.0 30.0 41.0 42-43 36.25412781297392 39.0 35.0 41.0 30.0 41.0 44-45 36.13915348692559 39.0 35.0 41.0 30.0 41.0 46-47 35.82783738548715 39.0 35.0 41.0 28.5 41.0 48-49 35.61313677001456 39.0 35.0 41.0 27.0 41.0 50-51 34.44211698493672 38.0 34.0 40.0 24.5 40.5 52-53 34.601293813338415 38.5 34.5 39.5 25.0 40.5 54-55 35.177969559103595 39.0 35.0 40.5 26.0 41.0 56-57 35.178522163534126 39.0 35.0 41.0 25.5 41.0 58-59 35.066064235875984 39.0 35.0 41.0 25.5 41.0 60-61 34.78408800204656 39.0 35.0 40.5 23.5 41.0 62-63 34.44325145424419 38.0 34.5 40.0 22.5 41.0 64-65 33.990317300280736 38.0 34.0 40.0 22.0 41.0 66-67 33.57429979751771 37.0 34.0 39.5 21.5 41.0 68-69 33.212134892329914 37.0 33.5 39.0 20.5 41.0 70-71 32.710453670681204 36.0 33.0 39.0 18.5 40.5 72-73 32.19524074659283 35.5 32.5 38.0 15.0 40.0 74-75 31.702043382373844 35.0 32.0 37.0 10.0 39.5 76-77 31.219885566574206 35.0 31.5 37.0 7.0 39.0 78-79 30.74564897162071 35.0 31.0 36.0 2.0 39.0 80-81 30.298994109454135 35.0 31.0 36.0 2.0 37.5 82-83 29.892501299999836 34.5 30.5 35.5 2.0 37.0 84-85 29.497570546936263 34.0 30.0 35.0 2.0 37.0 86-87 29.10557169060171 34.0 29.5 35.0 2.0 36.0 88-89 28.57651355929086 34.0 29.0 35.0 2.0 36.0 90-91 28.057152339916097 34.0 28.5 35.0 2.0 36.0 92-93 27.67995843344586 34.0 27.5 35.0 2.0 35.0 94-95 27.21845096868798 34.0 26.5 35.0 2.0 35.0 96-97 26.580320761373535 34.0 25.0 35.0 2.0 35.0 98-99 25.93335222722158 33.0 24.0 35.0 2.0 35.0 100-101 23.383773326845873 30.0 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34866273646671075 1101 2 0.26721872272329605 1101 3 0.22784445941353582 1101 4 0.20942765716720402 1101 5 0.09749557594123104 1101 6 0.21747898326474058 1101 7 0.36005557318007675 1101 8 0.5078336004405486 1101 9 0.3060311801507112 1101 10-11 0.48587961190482076 1101 12-13 0.5024764036403653 1101 14-15 0.3322751227146199 1101 16-17 0.2964383217293616 1101 18-19 0.5576498738387912 1101 20-21 0.6508141927585029 1101 22-23 0.4335160136091858 1101 24-25 0.6614069877335069 1101 26-27 0.4843000733237446 1101 28-29 0.31694335724888845 1101 30-31 0.5006367971213663 1101 32-33 0.6077892918536776 1101 34-35 0.4285291933923432 1101 36-37 0.4357932838234362 1101 38-39 0.20325914143409562 1101 40-41 0.22698021345782848 1101 42-43 0.127159396781245 1101 44-45 0.254580068877857 1101 46-47 0.6434873774943881 1101 48-49 0.8004414647357478 1101 50-51 0.6202751268819142 1101 52-53 0.4133717730924147 1101 54-55 0.4824170799362193 1101 56-57 0.5308691809422115 1101 58-59 0.4808323722209238 1101 60-61 0.5230545838454788 1101 62-63 0.6710227508227646 1101 64-65 0.44575426286606046 1101 66-67 0.6452167578493544 1101 68-69 0.6450581309903214 1101 70-71 0.7253015841690882 1101 72-73 0.264049702900941 1101 74-75 0.4257085688272504 1101 76-77 0.3460397357601046 1101 78-79 0.10302429209228237 1101 80-81 0.3189869268582939 1101 82-83 0.20477536706909305 1101 84-85 0.3701033289822817 1101 86-87 0.48260025045246024 1101 88-89 0.4871391741600206 1101 90-91 0.10977238367146924 1101 92-93 0.27985401072228555 1101 94-95 0.06948256856795965 1101 96-97 -0.0996505704577011 1101 98-99 0.11021603339185404 1101 100-101 0.7861595594048651 1102 1 -0.05083069161552345 1102 2 -0.12022511666701519 1102 3 -0.17297493836449718 1102 4 0.0968457495731343 1102 5 0.1452337033630542 1102 6 0.04909370539041191 1102 7 0.1393524230365415 1102 8 0.038485385350234935 1102 9 -0.08219472389027516 1102 10-11 -0.16761908143571702 1102 12-13 -0.02052691772061621 1102 14-15 0.01350009986417433 1102 16-17 0.15569082240521226 1102 18-19 0.1154454170357937 1102 20-21 0.1754057812187071 1102 22-23 -0.07357888977525562 1102 24-25 0.136860456495981 1102 26-27 0.07180441812736404 1102 28-29 -0.07400368120423195 1102 30-31 0.11865131842055376 1102 32-33 0.1433233486772778 1102 34-35 -0.11369103343859166 1102 36-37 -0.0086994362167232 1102 38-39 -0.03628597371928777 1102 40-41 -0.1514521813979215 1102 42-43 -0.22714485533322204 1102 44-45 0.06061194364453115 1102 46-47 -0.13783221982277638 1102 48-49 -0.08245803191070422 1102 50-51 0.05472008626604463 1102 52-53 -0.12413836478268792 1102 54-55 0.07733655624255675 1102 56-57 0.1261458966568867 1102 58-59 0.10657903476808173 1102 60-61 -0.06048150698988053 1102 62-63 -0.09590106385044805 1102 64-65 0.07051610326050195 1102 66-67 0.009575207365955407 1102 68-69 -0.008340792785730855 1102 70-71 -0.13336517261202374 1102 72-73 0.015367626664122724 1102 74-75 0.016651745232710624 1102 76-77 -0.17022431243825054 1102 78-79 -0.1206156234576099 1102 80-81 -0.047925301671938314 1102 82-83 -0.3187622042178653 1102 84-85 -0.5538658261304228 1102 86-87 -0.3061553977922422 1102 88-89 -0.20600665675953067 1102 90-91 -0.41040520615332454 1102 92-93 -0.6091620039019965 1102 94-95 -0.637112843319418 1102 96-97 -0.48326293673430243 1102 98-99 -0.10965999487833855 1102 100-101 0.13076041677944517 1103 1 -0.1656825371909747 1103 2 -0.08233448921529885 1103 3 0.012288701822040338 1103 4 0.20374539621180787 1103 5 0.1748457590723831 1103 6 0.1856713099395435 1103 7 0.0378096838900035 1103 8 0.04822635831778399 1103 9 -0.1930077059962585 1103 10-11 0.2470265843961812 1103 12-13 0.34209052941640294 1103 14-15 0.1853903005206874 1103 16-17 0.25441231970322775 1103 18-19 0.18555594444169543 1103 20-21 0.3741432833369842 1103 22-23 0.49801129895610075 1103 24-25 0.3039570565700416 1103 26-27 0.17835415546001343 1103 28-29 0.24381752926228728 1103 30-31 0.40940241522147147 1103 32-33 0.12825841931815773 1103 34-35 0.1810910813316653 1103 36-37 0.19788513513653072 1103 38-39 0.22564075558585017 1103 40-41 -0.014356773839885761 1103 42-43 -0.0920265138091878 1103 44-45 -0.09438218792136865 1103 46-47 0.010727032532436453 1103 48-49 -0.16677676006099063 1103 50-51 -0.11632123127586169 1103 52-53 0.011587218451786896 1103 54-55 -0.14649556260735608 1103 56-57 0.11977221234725732 1103 58-59 0.19680699362000098 1103 60-61 -0.1007148007818941 1103 62-63 -0.21681591193017624 1103 64-65 -0.20405286496207964 1103 66-67 -0.4286006824706732 1103 68-69 -0.5021773678797175 1103 70-71 -0.4317593556444024 1103 72-73 -0.2766450265584979 1103 74-75 -0.08745804155797998 1103 76-77 -0.18955719387077608 1103 78-79 0.012955585439815565 1103 80-81 -0.11691392255854538 1103 82-83 -0.18217361596854076 1103 84-85 -0.22541710118110458 1103 86-87 -0.1675348250079658 1103 88-89 -0.14782913505887763 1103 90-91 -0.5719722977322803 1103 92-93 -0.4433159765494459 1103 94-95 -0.28530806446837786 1103 96-97 -0.7146173211135967 1103 98-99 -0.8212201824171679 1103 100-101 -0.9396372559313946 1104 1 0.2863428380217705 1104 2 0.26782487949892086 1104 3 0.19469625249510258 1104 4 0.3334993804296644 1104 5 0.3855342938085755 1104 6 0.40037964971983087 1104 7 0.4501476074549089 1104 8 0.2775130672764732 1104 9 0.4653583778657193 1104 10-11 0.23403416601462368 1104 12-13 0.4273589806000686 1104 14-15 0.3339111112896802 1104 16-17 0.3167858304567446 1104 18-19 0.45676126704729114 1104 20-21 0.43209315181911734 1104 22-23 0.325770853342604 1104 24-25 0.1369417386062466 1104 26-27 0.3651791593281857 1104 28-29 0.4741966101844639 1104 30-31 0.4912715162581449 1104 32-33 0.6235779303811242 1104 34-35 0.5014949185589543 1104 36-37 0.6164121349818004 1104 38-39 0.41706269586126155 1104 40-41 0.5216327047304432 1104 42-43 0.7142492095390978 1104 44-45 0.8863001577385319 1104 46-47 0.7971512937114582 1104 48-49 0.7706191860940521 1104 50-51 0.8345467866724618 1104 52-53 0.8907408622704551 1104 54-55 0.864762367146632 1104 56-57 0.8416784478416091 1104 58-59 0.9310434163339139 1104 60-61 0.7260885413192995 1104 62-63 0.6471017733552884 1104 64-65 0.40598117495746067 1104 66-67 0.8048930510895929 1104 68-69 0.8084554651699492 1104 70-71 0.7157062176187878 1104 72-73 0.5810681097559041 1104 74-75 0.6064178674626 1104 76-77 0.5184437344906669 1104 78-79 0.5659804964718411 1104 80-81 0.5577000338084694 1104 82-83 0.6904196069929291 1104 84-85 0.9128453156531826 1104 86-87 0.7833270038640734 1104 88-89 0.8322312084348553 1104 90-91 0.9471271916689261 1104 92-93 1.037570608627714 1104 94-95 0.7191604486060434 1104 96-97 0.9035785631506776 1104 98-99 0.8462268236171795 1104 100-101 1.1930240468691622 1105 1 0.010813570853237309 1105 2 0.07917224468558359 1105 3 -0.04254449109070535 1105 4 -0.08581234180109476 1105 5 0.0051778642495037275 1105 6 -8.15618370502591E-4 1105 7 0.063003652930405 1105 8 0.13335267397489048 1105 9 0.15304312206605886 1105 10-11 -0.029798988079704714 1105 12-13 0.005194285562424739 1105 14-15 0.11459631639030476 1105 16-17 0.0846595437767661 1105 18-19 0.14244263131216428 1105 20-21 -0.05032196571045944 1105 22-23 -0.08674719858549906 1105 24-25 0.07004359757075207 1105 26-27 0.023175779392708762 1105 28-29 0.17285692666932562 1105 30-31 0.149182101601518 1105 32-33 0.06400656765259782 1105 34-35 -0.1463173413888157 1105 36-37 -0.16537769448509465 1105 38-39 -0.2791471674049859 1105 40-41 -0.05814913579805392 1105 42-43 0.10693164923798548 1105 44-45 0.07965551186359221 1105 46-47 0.08177565997355885 1105 48-49 -0.05838987827836206 1105 50-51 0.033651103771859425 1105 52-53 0.03320360616351792 1105 54-55 -0.11905695993894483 1105 56-57 -0.10109156291273536 1105 58-59 -0.060838593191370194 1105 60-61 0.11050396611572921 1105 62-63 -0.2559555319143314 1105 64-65 0.0018561173449214152 1105 66-67 -0.2542957643966588 1105 68-69 -0.12884826812241812 1105 70-71 -0.26735723298806846 1105 72-73 -0.2668271122443997 1105 74-75 -0.1135636965257234 1105 76-77 -0.19296815673686396 1105 78-79 -0.21387816077197286 1105 80-81 -0.32116615147344874 1105 82-83 -0.2884573326291324 1105 84-85 -0.18369332315462117 1105 86-87 -0.187991170970772 1105 88-89 -0.12484207471155884 1105 90-91 -0.1732323105587561 1105 92-93 -0.19636855253375174 1105 94-95 -0.2172491381200281 1105 96-97 0.07803700530768864 1105 98-99 -0.2617436787641374 1105 100-101 0.05935988954852789 1106 1 0.21493151380690634 1106 2 0.17552565125715702 1106 3 0.384762730679558 1106 4 0.19661544981148893 1106 5 0.24636412493870097 1106 6 0.28546866935764115 1106 7 0.3720884647942526 1106 8 0.15906575314794935 1106 9 0.16685757554280656 1106 10-11 0.1574910316064333 1106 12-13 0.21564812249661003 1106 14-15 0.18671465679285149 1106 16-17 0.33391122206673884 1106 18-19 0.22430157422235908 1106 20-21 0.4021611582377034 1106 22-23 0.3594730005115565 1106 24-25 0.27330454138763116 1106 26-27 0.2555436431671083 1106 28-29 0.1774089583161924 1106 30-31 0.10975703623980593 1106 32-33 0.2703648181323288 1106 34-35 0.2447149078613151 1106 36-37 0.19316310923105817 1106 38-39 0.32736757856929444 1106 40-41 0.3676707577601661 1106 42-43 0.43072051694339564 1106 44-45 0.6431122613528117 1106 46-47 0.3426666906702778 1106 48-49 0.32572272395604784 1106 50-51 0.378709696430235 1106 52-53 0.2148579250044591 1106 54-55 0.3309700541642755 1106 56-57 0.45783799548430437 1106 58-59 0.7375399396012696 1106 60-61 0.5088408909792648 1106 62-63 0.7339436775350165 1106 64-65 0.5753018748886873 1106 66-67 0.4132693023312868 1106 68-69 0.6821942899579057 1106 70-71 0.7518352005789737 1106 72-73 0.6505951212940744 1106 74-75 0.3486445045834081 1106 76-77 0.2801202073263056 1106 78-79 0.4972634490201706 1106 80-81 0.4517383924850158 1106 82-83 0.6648843429904865 1106 84-85 0.30371717322176295 1106 86-87 0.9939115534170639 1106 88-89 0.98489262816436 1106 90-91 0.7864669945267266 1106 92-93 1.0731188626202268 1106 94-95 1.1040370437004121 1106 96-97 0.889511168025745 1106 98-99 1.2157507786870951 1106 100-101 1.3817716590033413 1107 1 0.10565479820330026 1107 2 0.0865249234163592 1107 3 0.18352658480369044 1107 4 0.0638170498777697 1107 5 0.1744143992104199 1107 6 0.11426927056597691 1107 7 0.06319401354479481 1107 8 0.005216185414369079 1107 9 0.20752789916567593 1107 10-11 0.10735286020288726 1107 12-13 0.16874677155307438 1107 14-15 0.10448935752010158 1107 16-17 0.2036706203850187 1107 18-19 0.20286725445763665 1107 20-21 0.18562704143996456 1107 22-23 0.1414684086053768 1107 24-25 0.27590967887853424 1107 26-27 0.3178361641591039 1107 28-29 0.4655185249846383 1107 30-31 0.4956354454046803 1107 32-33 0.30766517968577034 1107 34-35 0.4900046535904252 1107 36-37 0.042406864889578344 1107 38-39 0.07129780053115553 1107 40-41 0.07437754443180467 1107 42-43 0.0432976651093 1107 44-45 0.39811394781320075 1107 46-47 0.4083839097266875 1107 48-49 0.027290244504236227 1107 50-51 0.15232310053572462 1107 52-53 0.07704008639576188 1107 54-55 0.1998714287774277 1107 56-57 0.13059515110614228 1107 58-59 0.3371786820343914 1107 60-61 -0.027653224161596768 1107 62-63 -0.028641163257212554 1107 64-65 0.1871186365297035 1107 66-67 0.11643748089489492 1107 68-69 -0.07888752999913606 1107 70-71 0.11523190931838911 1107 72-73 0.10206796336446189 1107 74-75 0.165561477475773 1107 76-77 0.3475727053587683 1107 78-79 0.3233879038792473 1107 80-81 0.42457750416420126 1107 82-83 0.6532596011372718 1107 84-85 0.7113257723945097 1107 86-87 0.7085151168782744 1107 88-89 0.5964223956695207 1107 90-91 0.5803756179608328 1107 92-93 0.5199465418732139 1107 94-95 0.4877067815002043 1107 96-97 0.7696230661516985 1107 98-99 0.9840148487965088 1107 100-101 1.0560850921041194 1108 1 0.14343876146386592 1108 2 0.041665584796188426 1108 3 0.10217873309188619 1108 4 0.04111749511582019 1108 5 0.13780917889636157 1108 6 0.05167043413698025 1108 7 0.24877016792510886 1108 8 0.20321874130254258 1108 9 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-1.5782401754167878 2316 76-77 -1.498999073031193 2316 78-79 -1.5158376853463373 2316 80-81 -1.490711752034148 2316 82-83 -1.1145702121470862 2316 84-85 -1.5322463120866665 2316 86-87 -1.3689259605628017 2316 88-89 -1.5060416386126931 2316 90-91 -2.1378674184640225 2316 92-93 -2.5753243488522237 2316 94-95 -2.4225319696476397 2316 96-97 -2.2403248983417186 2316 98-99 -2.8808458797005834 2316 100-101 -3.499929600274207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2400.0 3 504.0 4 394.0 5 429.0 6 487.0 7 562.0 8 705.0 9 903.0 10 1138.0 11 1582.0 12 2356.0 13 3672.0 14 3718.0 15 3585.0 16 4893.0 17 6772.0 18 5755.0 19 3456.0 20 3291.0 21 3443.0 22 3718.0 23 4356.0 24 4977.0 25 5811.0 26 6816.0 27 7861.0 28 9267.0 29 11053.0 30 13161.0 31 16139.0 32 20081.0 33 25292.0 34 34011.0 35 53426.0 36 70070.0 37 116055.0 38 124584.0 39 21351.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74159066786722 22.329478479722194 16.09866681422087 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33.20254185661974 17.281378239243804 20-21 18.530255724491358 30.458904917752932 31.146270660093705 19.864568697662 22-23 17.921787709497206 29.684179691586632 31.05977862866947 21.33425397024669 24-25 19.651483305333805 30.49085809136133 31.66960054843918 18.18805805486568 26-27 20.060755416339507 28.22657467692818 34.04605290693489 17.666616999797427 28-29 17.390147095504105 29.833335286348255 34.58091926738504 18.195598350762594 30-31 17.875095201827875 31.013031142505625 32.77629453562432 18.33557912004218 32-33 17.884933726338907 30.981827676764635 32.44714593930508 18.686092657591384 34-35 19.399839699877887 28.88348027511194 32.229136855180165 19.48754316983001 36-37 20.051823363237034 28.657260190792734 33.253492946825126 18.03742349914511 38-39 19.992196756104065 28.901685012978778 33.594394445570266 17.511723785346884 40-41 17.724288472416827 29.852176420008252 34.82692276980724 17.596612337767674 42-43 17.050656761367314 31.292158967947625 32.16352487857292 19.493659392112136 44-45 18.084778047354945 30.68467460821374 31.264270186400694 19.96627715803062 46-47 19.195281775825492 30.393328293789168 30.182020599346988 20.22936933103835 48-49 19.127711236540012 29.873076829267266 31.240627525784554 19.75858440840817 50-51 19.04544846732001 30.60137368898652 32.2319039981969 18.12127384549657 52-53 17.916737802544745 31.515618915779886 32.21677337749224 18.350869904183124 54-55 17.074758252302267 30.003203961138723 32.73952132316026 20.182516463398738 56-57 19.16190216783646 31.54035175325991 29.93845452856472 19.359291550338916 58-59 19.20348090412046 29.61189533077332 31.07737546823406 20.107248296872164 60-61 18.637218313586647 29.683291297063956 32.137246039040804 19.542244350308593 62-63 20.154543390705534 29.25746861625648 31.78632988875903 18.80165810427895 64-65 19.375902655753066 30.3115692183979 32.091940719331994 18.220587406517037 66-67 18.180693964697745 29.995558195213956 31.27392307265211 20.549824767436185 68-69 17.410850991516668 30.97383324752415 31.986045071692732 19.62927068926645 70-71 19.200320336218436 30.588491618513885 30.30971162169369 19.901476423573985 72-73 18.592350649754845 28.85560881974171 31.31600485518151 21.236035675321933 74-75 18.34619997947617 31.5429377000863 30.45382895575769 19.657033364679837 76-77 19.700569442844206 29.0893540006913 31.19253324272932 20.01754331373517 78-79 18.55716302372919 29.094174354852402 32.430200970422376 19.918461650996026 80-81 18.86570687759158 30.92822768591289 30.838688848757556 19.367376587737972 82-83 18.682247328572917 30.326877734912365 31.016639030247582 19.974235906267133 84-85 18.67986410118407 29.226823196986008 30.774270048439185 21.319042653390742 86-87 19.171523397657165 29.94274509078464 31.105669741171482 19.78006177038671 88-89 20.297404397319628 28.337599908501304 31.21577208751581 20.14922360666326 90-91 18.958694132697907 28.15063532930556 32.52446987200892 20.366200665987613 92-93 19.24206024757156 30.34686262436618 31.4063935237057 19.004683604356558 94-95 18.827407649162712 30.026176865438124 30.801557153383648 20.344858332015516 96-97 18.57027621957938 28.855310870042466 30.537912796404665 22.036500113973485 98-99 19.606556659996283 29.2253689950767 29.785412129669588 21.382662215257433 100-101 20.37107118168561 28.394043215950244 30.855656976969097 20.379228625395047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9504.0 1 5493.0 2 1327.0 3 1052.5 4 838.5 5 851.0 6 1357.0 7 2304.0 8 3186.0 9 3269.0 10 2453.5 11 1725.0 12 1566.0 13 1537.5 14 1442.5 15 1355.0 16 1427.5 17 1480.5 18 1414.5 19 1390.0 20 1488.0 21 1687.0 22 1923.5 23 2215.0 24 2444.5 25 3072.0 26 4671.5 27 6217.5 28 6381.0 29 6739.0 30 8639.0 31 10623.0 32 13016.5 33 16340.5 34 19985.5 35 22571.5 36 26290.5 37 30661.5 38 29673.0 39 25908.0 40 23096.0 41 20285.5 42 17534.0 43 16737.0 44 16909.0 45 16688.5 46 17903.5 47 25153.5 48 56218.5 49 54660.5 50 20499.0 51 9584.0 52 5250.5 53 3562.5 54 2637.5 55 2132.5 56 1636.5 57 1301.0 58 1218.0 59 1046.0 60 830.0 61 646.0 62 466.0 63 310.0 64 209.0 65 149.0 66 121.5 67 97.5 68 72.5 69 60.0 70 59.5 71 52.0 72 38.5 73 32.5 74 25.0 75 21.0 76 15.0 77 11.5 78 7.5 79 4.5 80 8.5 81 6.0 82 2.5 83 3.0 84 1.5 85 0.5 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.281903500719807 2 0.08142764226011032 3 0.0844372881752684 4 0.09981992285274305 5 0.10199355601369055 6 0.07624436318400474 7 0.08560770603116322 8 0.0822636550143209 9 0.08694532643790014 10-11 0.08059162950589975 12-13 0.08510609837863686 14-15 0.0951382514291638 16-17 0.08218005373889985 18-19 0.09380063102242688 20-21 0.09672667566216389 22-23 0.11110609503458585 24-25 0.12464950165279721 26-27 0.12774274884337636 28-29 0.1187138110979021 30-31 0.11068808865748055 32-33 0.10533760703053285 34-35 0.38782631667828726 36-37 0.5948230746208264 38-39 0.5772668067824043 40-41 0.5371381945802965 42-43 0.5648938180200876 44-45 0.5893053904430366 46-47 0.5762635914773516 48-49 0.5991703409427215 50-51 0.5929838465615632 52-53 0.5948230746208264 54-55 0.5852925292228258 56-57 0.5540256522153503 58-59 0.5499291897197184 60-61 0.49425074028929383 62-63 0.5721671289817197 64-65 0.6103729118491431 66-67 0.6226622993360387 68-69 0.624501527395302 70-71 0.6189002419420911 72-73 0.6120449373575644 74-75 0.6096205003703536 76-77 0.5933182516632474 78-79 0.5663986409776668 80-81 0.5623021784820349 82-83 0.5770160029561411 84-85 0.5880513713117207 86-87 0.5777684144349305 88-89 0.5907266121251945 90-91 0.6055240378747219 92-93 0.6124629437346697 94-95 0.6117941335313012 96-97 0.6087008863407221 98-99 0.6117105322558801 100-101 0.5898905993709841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 598077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.034697999252757 #Duplication Level Percentage of deduplicated Percentage of total 1 75.67074003595286 18.187233841440918 2 10.49297616877159 5.043910266595627 3 3.75058964137243 2.704328680485362 4 2.0107844604723755 1.933143889961757 5 1.1926036621172356 1.4331934425895314 6 0.8931791754184006 1.2880375044241714 7 0.6095169438317543 1.0254688969296601 8 0.5288696573771376 1.0168977996822224 9 0.39062268708381415 0.8449648484144473 >10 3.4641822251849974 17.52068690236791 >50 0.5586444042551374 9.413545777301522 >100 0.3912603130925495 17.352652495926733 >500 0.018830710255791153 3.0179400650759747 >1k 0.02301531253485585 11.818309617202651 >5k 0.0041846022790647 7.3996859716015235 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9977 1.668179849751788 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7947 1.328758671542293 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7117 1.189980554343337 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6851 1.1455046758193344 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 1.0463535631699596 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5994 1.0022120897476412 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4804 0.8032410542455235 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4593 0.7679613160178371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3998 0.6684757982667784 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3571 0.597080309057195 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3243 0.542237872380981 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3212 0.5370545933048755 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2974 0.4972603862044519 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2809 0.46967196531550287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2599 0.43455942963865857 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2549 0.42619930209655277 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2460 0.41131827507160446 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2257 0.377376157250655 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2083 0.34828291340412687 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1971 0.3295562277098099 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1960 0.3277169996505467 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1844 0.30832150375286127 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1843 0.30815430120201914 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1807 0.30213500937170296 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1747 0.292102856321176 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1733 0.28976202060938644 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1678 0.28056588031307006 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1529 0.25565270023759484 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1496 0.250135016059805 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1412 0.23609000178906728 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1376 0.23007070995875115 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1256 0.21000640385769723 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1193 0.19947264315464397 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1124 0.18793566714653798 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1101 0.18409000847716933 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1100 0.1839228059263272 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1096 0.18325399572295875 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1075 0.1797427421552743 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1011 0.16904177890137892 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 995 0.16636653808790508 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.1613504615626416 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 932 0.15583277738485177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 826 0.13810930699558752 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 796 0.13309323047032404 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 783 0.13091959730937655 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 720 0.12038583660632327 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 713 0.11921541875042846 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 709 0.11854660854706 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 674 0.11269451926758595 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 661 0.11052088610663845 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 0.10634082233558556 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 635 0.10617361978474343 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 626 0.1046687968271644 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 625 0.10450159427632227 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 613 0.1024951636662169 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 608 0.10165915091200632 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.360127542105783E-5 0.0 12-13 0.0 0.0 0.0 1.6720255084211566E-4 0.0 14-15 0.0 0.0 0.0 2.508038262631735E-4 0.0 16-17 0.0 0.0 0.0 5.01607652526347E-4 0.0 18-19 0.0 0.0 0.0 6.688102033684626E-4 0.0 20-21 0.0 0.0 0.0 0.001421221682157983 0.0 22-23 0.0 0.0 0.0 0.002758842088894908 0.0 24-25 0.0 0.0 0.0 0.005350481626947701 0.0 26-27 0.0 0.0 0.0 0.01036655815221117 0.0 28-29 0.0 0.0 0.0 0.03068166807952822 0.0 30-31 0.0 0.0 0.0 0.07674597083653108 0.0 32-33 0.0 0.0 0.0 0.15039869448248303 0.0 34-35 0.0 0.0 0.0 0.2610867831399636 0.0 36-37 0.0 0.0 0.0 0.4128230980291835 0.0 38-39 0.0 0.0 0.0 0.5976655179851424 0.0 40-41 0.0 0.0 0.0 0.7937105088475229 0.0 42-43 0.0 0.0 0.0 0.9883342780277455 0.0 44-45 0.0 0.0 0.0 1.217568975232286 0.0 46-47 0.0 0.0 0.0 1.4917811586133558 0.0 48-49 0.0 0.0 0.0 1.7741862669856892 0.0 50-51 0.0 0.0 0.0 2.0518261026590223 0.0 52-53 0.0 0.0 0.0 2.305054365909406 0.0 54-55 0.0 0.0 0.0 2.5828614041335816 0.0 56-57 0.0 0.0 0.0 2.9196073415296024 0.0 58-59 0.0 0.0 0.0 3.293890251589678 0.0 60-61 0.0 0.0 0.0 3.771253534243918 0.0 62-63 0.0 0.0 0.0 4.19494479807784 0.0 64-65 0.0 0.0 0.0 4.597986546882759 0.0 66-67 0.0 0.0 0.0 5.074513816782789 0.0 68-69 0.0 0.0 0.0 5.599613427702453 0.0 70-71 0.0 0.0 0.0 6.200539395429017 0.0 72-73 0.0 0.0 0.0 6.813336744265371 0.0 74-75 0.0 0.0 0.0 7.457317368833779 0.0 76-77 0.0 0.0 0.0 8.0469571643785 0.0 78-79 0.0 0.0 0.0 8.78916928756665 0.0 80-81 0.0 0.0 0.0 9.589818702274123 0.0 82-83 0.0 0.0 0.0 10.336043686682483 0.0 84-85 0.0 0.0 0.0 11.242699518623855 0.0 86-87 0.0 0.0 0.0 12.21907881426639 0.0 88-89 0.0 0.0 0.0 13.086191242933602 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 20 0.0021069155 70.96415 7 GTACGTA 25 0.0050972337 56.780827 3 CCGTATC 30 8.200895E-5 39.65675 94-95 GACATAT 175 0.0 37.923687 1 CAGGGGC 50 0.0016474082 37.84755 6 CATATAG 195 0.0 36.397964 3 CTTTGCG 510 0.0 33.400486 2 GCTTTGC 530 0.0 32.199356 1 GTTGCAA 140 1.7695129E-8 30.415752 8 GGGTTGC 125 2.2281165E-7 30.278042 6 AAATCAG 95 3.581006E-5 29.884644 5 GCCGTAT 40 4.445231E-4 29.742563 94-95 GGTTGCA 130 3.1413765E-7 29.113499 7 TTGCGTT 610 0.0 28.70069 4 TTTGCGT 625 0.0 27.254795 3 GTCTATA 70 0.008471044 27.088348 1 CCCTTGG 285 0.0 26.561905 8 ACAGGGG 90 9.1459084E-4 26.287416 5 CTTAATC 200 7.4942363E-10 26.022367 9 CGCAGAA 165 8.722236E-8 25.807306 9 >>END_MODULE