##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938900_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 598077 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0960378011527 33.0 31.0 34.0 30.0 34.0 2 32.32721371997251 34.0 31.0 34.0 30.0 34.0 3 32.39656599401081 34.0 31.0 34.0 30.0 34.0 4 35.961349458347335 37.0 35.0 37.0 35.0 37.0 5 35.889748310000215 37.0 35.0 37.0 35.0 37.0 6 35.95843344586065 37.0 35.0 37.0 35.0 37.0 7 35.92605467189007 37.0 35.0 37.0 35.0 37.0 8 35.92518856267671 37.0 35.0 37.0 35.0 37.0 9 37.55278835333912 39.0 37.0 39.0 35.0 39.0 10-11 37.48181505057041 39.0 37.0 39.0 34.5 39.0 12-13 37.438922580202885 39.0 37.0 39.0 34.0 39.0 14-15 38.54652661781008 40.0 38.0 41.0 34.0 41.0 16-17 38.523665849046196 40.0 38.0 41.0 34.0 41.0 18-19 38.689167949946246 40.0 38.0 41.0 34.0 41.0 20-21 38.704079909443095 40.0 38.0 41.0 34.0 41.0 22-23 38.647523646620755 40.0 38.0 41.0 34.0 41.0 24-25 38.55073677804029 40.0 38.0 41.0 34.0 41.0 26-27 38.293705492771 40.0 37.5 41.0 34.0 41.0 28-29 38.10672037212599 40.0 37.0 41.0 33.0 41.0 30-31 37.96885100079086 40.0 37.0 41.0 33.0 41.0 32-33 37.78148716636821 40.0 36.0 41.0 33.0 41.0 34-35 37.71989643474001 40.0 36.0 41.0 33.0 41.0 36-37 37.556027902761684 40.0 36.0 41.0 33.0 41.0 38-39 37.34845764006975 40.0 36.0 41.0 32.5 41.0 40-41 37.21820434492548 40.0 36.0 41.0 32.0 41.0 42-43 37.100350790951666 40.0 36.0 41.0 32.0 41.0 44-45 36.871918665991174 40.0 35.5 41.0 31.0 41.0 46-47 36.5140383261687 40.0 35.0 41.0 30.5 41.0 48-49 36.18438595699216 39.5 35.0 41.0 30.0 41.0 50-51 35.95150875221752 39.0 35.0 41.0 28.5 41.0 52-53 35.711527111057606 39.0 35.0 41.0 28.0 41.0 54-55 35.550421601231946 39.0 35.0 40.0 28.0 41.0 56-57 35.363768377650366 39.0 35.0 40.0 27.0 41.0 58-59 35.14619773039257 38.5 35.0 40.0 26.5 41.0 60-61 34.714699779459835 38.0 34.0 40.0 26.0 41.0 62-63 34.40438104123716 38.0 34.0 40.0 26.0 41.0 64-65 34.111371947090426 37.5 34.0 40.0 25.0 41.0 66-67 33.64317052820958 37.0 33.0 39.0 22.5 41.0 68-69 33.2208185568079 36.0 33.0 39.0 22.0 40.0 70-71 32.71081817224204 36.0 32.5 38.5 21.5 40.0 72-73 32.14747264984275 35.0 32.0 37.5 20.5 39.5 74-75 31.656215671226278 35.0 31.0 37.0 18.5 39.0 76-77 29.57619587444426 33.0 28.5 35.0 12.5 37.5 78-79 30.540527390285867 34.0 30.5 36.0 12.0 38.0 80-81 30.538327004716784 34.0 31.0 36.0 10.5 37.0 82-83 30.21057071246679 34.0 30.5 35.0 7.0 37.0 84-85 29.818878672813035 34.0 30.0 35.0 2.0 36.5 86-87 29.275590768412762 34.0 29.5 35.0 2.0 36.0 88-89 28.638403583485072 34.0 29.0 35.0 2.0 36.0 90-91 28.129081205262867 33.5 28.5 35.0 2.0 35.0 92-93 27.83150413742712 33.0 27.0 35.0 2.0 35.0 94-95 27.478897366058217 33.0 27.0 35.0 2.0 35.0 96-97 26.822256164340043 33.0 25.5 35.0 2.0 35.0 98-99 25.987212348911594 33.0 24.5 34.5 2.0 35.0 100-101 21.70628781912697 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24721310197396207 1101 2 0.21028604855387556 1101 3 0.11718712474463189 1101 4 0.09073873956434397 1101 5 0.1573957856208068 1101 6 0.1866241726032385 1101 7 0.17323294813922274 1101 8 0.27280772635622696 1101 9 0.3159470592303393 1101 10-11 0.28032726634518923 1101 12-13 0.18463266536030432 1101 14-15 0.31874709712358396 1101 16-17 0.2559276632910681 1101 18-19 0.34954782854830313 1101 20-21 0.3574084290756332 1101 22-23 0.36553905913645934 1101 24-25 0.21596927694093182 1101 26-27 0.27360788941022207 1101 28-29 0.44628865840750365 1101 30-31 0.25247254149137177 1101 32-33 0.48310923228522995 1101 34-35 0.5065879379722489 1101 36-37 0.4443760043143925 1101 38-39 0.7195917083460657 1101 40-41 0.6795784911267759 1101 42-43 0.40414965863352137 1101 44-45 0.4865935016035934 1101 46-47 0.8798839673810122 1101 48-49 0.8238739217170092 1101 50-51 0.7144566069621661 1101 52-53 0.7259449650793712 1101 54-55 0.8580601914553938 1101 56-57 0.7799686899473315 1101 58-59 0.7029001903132297 1101 60-61 0.9824378337454718 1101 62-63 0.5834392750158202 1101 64-65 0.6114914267004607 1101 66-67 0.9182612852903489 1101 68-69 0.920294884181331 1101 70-71 0.7436179772557239 1101 72-73 0.6199911404677962 1101 74-75 0.32441906720343994 1101 76-77 0.25692590752126776 1101 78-79 0.7767962757450846 1101 80-81 0.966537590217694 1101 82-83 0.7637604703968783 1101 84-85 0.5926615838913456 1101 86-87 0.5937782452778215 1101 88-89 0.9888519379751557 1101 90-91 0.8257970224518907 1101 92-93 0.8292560277007013 1101 94-95 0.609242685141659 1101 96-97 0.8300937159803468 1101 98-99 1.1163771810040792 1101 100-101 1.8848239436144638 1102 1 0.005645239359871823 1102 2 -0.11360135988341113 1102 3 -0.07545503079290228 1102 4 -0.14135101442321485 1102 5 -0.09907958095188008 1102 6 0.05206721383703439 1102 7 0.11282086765415045 1102 8 -0.03423149917448143 1102 9 0.0828563912248299 1102 10-11 0.11333232562762419 1102 12-13 -0.0028295187563784907 1102 14-15 0.03736956909155964 1102 16-17 0.20402496114505908 1102 18-19 -0.22776231962559024 1102 20-21 -0.07403700662771229 1102 22-23 0.039739937108628 1102 24-25 -0.0014736613049350922 1102 26-27 -0.06165646775193778 1102 28-29 -0.10994919223423949 1102 30-31 -0.18350275427711438 1102 32-33 -0.14672637321947235 1102 34-35 -0.045243959966704494 1102 36-37 0.12903659469274942 1102 38-39 0.19281162621318515 1102 40-41 0.21765989021790944 1102 42-43 0.13770377235795905 1102 44-45 0.46261501320905296 1102 46-47 0.5463768419030899 1102 48-49 0.32127508548069983 1102 50-51 0.26124692352134105 1102 52-53 0.24105844491304396 1102 54-55 0.3213079804331187 1102 56-57 0.38879797456736753 1102 58-59 0.0498166670958895 1102 60-61 0.16572417883230628 1102 62-63 0.2410076010756299 1102 64-65 0.26366415034519974 1102 66-67 0.3289626975124449 1102 68-69 -0.006796092787993757 1102 70-71 -0.005645015316268598 1102 72-73 0.240046110279458 1102 74-75 0.44920665458227305 1102 76-77 -0.026638504996917334 1102 78-79 -0.10760298274176705 1102 80-81 0.02384233152486459 1102 82-83 -0.16731807077898608 1102 84-85 -0.18371941998357144 1102 86-87 0.08672839911605124 1102 88-89 0.28297422327748833 1102 90-91 0.5789043616297889 1102 92-93 0.4703366286353159 1102 94-95 0.3979613220963074 1102 96-97 0.06190668988577741 1102 98-99 0.5179963444845548 1102 100-101 1.0414508955577162 1103 1 -0.18719087755690822 1103 2 -0.07413221780454649 1103 3 -0.008322655238224286 1103 4 -0.1853736847804086 1103 5 -0.05799112764515968 1103 6 -0.06680466600756318 1103 7 -0.07527342898107037 1103 8 0.19093121649831346 1103 9 0.1760075834270296 1103 10-11 0.12251626808558314 1103 12-13 0.06175620745450061 1103 14-15 0.12825498085478415 1103 16-17 -0.04277093659982967 1103 18-19 -0.12195319750566824 1103 20-21 -0.11003059214679922 1103 22-23 -0.015308799138210816 1103 24-25 0.16923186883121133 1103 26-27 0.20568051844625046 1103 28-29 -0.0012592024647730682 1103 30-31 -0.00902510767609499 1103 32-33 0.0584852356101635 1103 34-35 0.07669080313057464 1103 36-37 -0.15898269896118222 1103 38-39 0.04590934348646414 1103 40-41 -0.0236159136320353 1103 42-43 -0.09103178407629997 1103 44-45 -0.032917759014111425 1103 46-47 0.034980468407070475 1103 48-49 -0.14869861640284654 1103 50-51 -0.27384442948223153 1103 52-53 -0.21524525753537915 1103 54-55 0.009938909277565244 1103 56-57 -0.09712562909052025 1103 58-59 -0.2301562816196494 1103 60-61 -0.11164092563837613 1103 62-63 -0.15763795178106932 1103 64-65 -0.07061691940442927 1103 66-67 -0.0969899031139434 1103 68-69 -0.37252663437855915 1103 70-71 -0.0830957926484217 1103 72-73 0.015242718512837428 1103 74-75 0.06009652258957843 1103 76-77 0.1628601361624611 1103 78-79 -0.02657151914802469 1103 80-81 0.19231817052265043 1103 82-83 0.2369856567750368 1103 84-85 0.26854541899538376 1103 86-87 1.3133772658235898E-4 1103 88-89 0.27891025420759163 1103 90-91 0.3236117872387574 1103 92-93 0.40341765068430746 1103 94-95 0.40408708860167053 1103 96-97 -0.11070686588317713 1103 98-99 -0.14368529139636976 1103 100-101 0.5080965362840217 1104 1 0.08216391124086186 1104 2 0.04484967914733318 1104 3 0.2188659568614213 1104 4 0.008536264159715756 1104 5 0.0895347891117595 1104 6 0.08978824750009551 1104 7 0.13288067901831369 1104 8 0.17400099833337634 1104 9 0.11353195291488305 1104 10-11 0.07931806291771437 1104 12-13 0.0994739855761182 1104 14-15 0.20619711299156762 1104 16-17 0.06562299810540395 1104 18-19 0.14624887583487833 1104 20-21 0.1125115709353608 1104 22-23 0.04113093473150542 1104 24-25 0.006007360089135716 1104 26-27 -0.07989893382049473 1104 28-29 0.10981769680801534 1104 30-31 0.028688028638313767 1104 32-33 0.06488009867685207 1104 34-35 0.008419102681862967 1104 36-37 0.2467530274816454 1104 38-39 0.3507151612181687 1104 40-41 0.28227763425594077 1104 42-43 0.27627596774174634 1104 44-45 0.37180295891872817 1104 46-47 0.40732922285546636 1104 48-49 0.4019713511044998 1104 50-51 0.6420367402531824 1104 52-53 0.34089894794195175 1104 54-55 0.4383220130993237 1104 56-57 0.4083501561485363 1104 58-59 0.5987761027219918 1104 60-61 0.55982279090194 1104 62-63 0.7078981769517156 1104 64-65 0.4846824771606322 1104 66-67 0.6077741383528732 1104 68-69 0.8004472167741596 1104 70-71 0.7059093133395109 1104 72-73 0.5253386491951844 1104 74-75 0.7173594036045863 1104 76-77 0.4719750982543651 1104 78-79 0.8288083354086808 1104 80-81 0.918462375999983 1104 82-83 0.9237255608443569 1104 84-85 0.9504083309557707 1104 86-87 0.7645859253793752 1104 88-89 0.9528078249951513 1104 90-91 1.1398766797035584 1104 92-93 1.287681924241479 1104 94-95 1.372903427763049 1104 96-97 1.0958442713595922 1104 98-99 1.1936209131526034 1104 100-101 1.5296113127036435 1105 1 -0.06480490117272097 1105 2 -0.0849152617037916 1105 3 -0.01654040356260822 1105 4 -0.040906046487080516 1105 5 -0.2283757962316102 1105 6 -0.08390663650666141 1105 7 -0.06552153330309096 1105 8 -0.037927101221086446 1105 9 -0.10147188792088002 1105 10-11 0.015509490172284757 1105 12-13 -0.14336055121909652 1105 14-15 -0.10553587210599602 1105 16-17 -0.1400383938134695 1105 18-19 -0.20480658583357325 1105 20-21 -0.21245693421135314 1105 22-23 -0.2858563136937491 1105 24-25 -0.32994132634502904 1105 26-27 -0.43577541254226304 1105 28-29 -0.339601568824186 1105 30-31 -0.27482556792030266 1105 32-33 -0.39555007492892 1105 34-35 0.016073120196075763 1105 36-37 -0.09229444524842734 1105 38-39 0.1947342117635671 1105 40-41 0.21144186839328682 1105 42-43 0.29104321309433345 1105 44-45 0.21685903512091187 1105 46-47 0.47856597197208117 1105 48-49 0.5749410853186703 1105 50-51 0.7122287820414854 1105 52-53 0.8205839994091519 1105 54-55 0.49162974086088695 1105 56-57 0.5907099656430788 1105 58-59 0.33644394724495896 1105 60-61 0.1470389531388392 1105 62-63 0.23339671743411827 1105 64-65 0.30417087783661856 1105 66-67 0.33430355915484 1105 68-69 0.48400229435904407 1105 70-71 0.4303877278425077 1105 72-73 0.26377773353993206 1105 74-75 0.5908392008084569 1105 76-77 0.0883785396432124 1105 78-79 0.13269372486942999 1105 80-81 0.17956085502902042 1105 82-83 0.2710984291805083 1105 84-85 0.12588229008927954 1105 86-87 -0.0193378724390314 1105 88-89 -0.04733812676395388 1105 90-91 -0.22356010013973915 1105 92-93 -0.3744013450779242 1105 94-95 -0.10029214759414629 1105 96-97 -0.363891479139717 1105 98-99 -0.17700898391299802 1105 100-101 0.31503918180795765 1106 1 -0.019399472269491014 1106 2 0.02616548872936164 1106 3 -0.057337909570918555 1106 4 -0.035382128137989355 1106 5 0.031611659288273586 1106 6 0.11344225529408902 1106 7 0.04231752474307626 1106 8 0.05396432071436408 1106 9 -0.11106344710039906 1106 10-11 0.1796512179570584 1106 12-13 0.1554852945086438 1106 14-15 0.061568091825527915 1106 16-17 -0.006028258867154079 1106 18-19 -0.06429288316260084 1106 20-21 0.04668594244908064 1106 22-23 0.1259876627822436 1106 24-25 0.09136534893302439 1106 26-27 0.14397208321931743 1106 28-29 0.23533462200095556 1106 30-31 0.04475282576522943 1106 32-33 0.19276120206189518 1106 34-35 0.24489499478853816 1106 36-37 0.14142101861788348 1106 38-39 0.3585825104960847 1106 40-41 0.4330180636897296 1106 42-43 0.3871233735643145 1106 44-45 0.19845994676927603 1106 46-47 0.1689812439451046 1106 48-49 0.3631908819366245 1106 50-51 0.3798559501561485 1106 52-53 0.3781007512582235 1106 54-55 0.3849209280530914 1106 56-57 0.45019626297209925 1106 58-59 0.563064327678056 1106 60-61 0.43400480358628357 1106 62-63 0.6498222237156739 1106 64-65 0.5574187803622834 1106 66-67 0.7673706845437493 1106 68-69 0.6547438470179117 1106 70-71 0.56361849038543 1106 72-73 0.8589139529317151 1106 74-75 0.7763981443396695 1106 76-77 0.3332924360705185 1106 78-79 0.6173251841899159 1106 80-81 0.8054357083144943 1106 82-83 1.0149534969641714 1106 84-85 0.9226617121737739 1106 86-87 0.9620658673064781 1106 88-89 1.1887977386499209 1106 90-91 1.5435612521800586 1106 92-93 1.5182160420683317 1106 94-95 1.5983531106544682 1106 96-97 1.3237758064707705 1106 98-99 1.143209054055788 1106 100-101 1.5156126881143521 1107 1 0.053825454206752 1107 2 0.09282801321262468 1107 3 0.09570097369643804 1107 4 -0.004987378059581715 1107 5 -0.23728705249268955 1107 6 -0.0843765892734396 1107 7 -0.049757294924013706 1107 8 -0.09066888894520275 1107 9 0.09279706783217279 1107 10-11 0.15445786675316242 1107 12-13 0.21534428275075612 1107 14-15 0.13649575155107385 1107 16-17 -0.023287849501087976 1107 18-19 0.17996575476914956 1107 20-21 0.19672618080214477 1107 22-23 0.1672852313205837 1107 24-25 0.01560185577059059 1107 26-27 0.0061646000691197855 1107 28-29 -0.0273774429958209 1107 30-31 0.045613808172788595 1107 32-33 0.1496100064502386 1107 34-35 0.014440183050773214 1107 36-37 -0.03698873799925195 1107 38-39 -0.11591051584228751 1107 40-41 -0.13246837657703026 1107 42-43 -0.10191463392711597 1107 44-45 -0.27367647308243903 1107 46-47 -0.004488958962710399 1107 48-49 0.040931971804248235 1107 50-51 -0.03404728843953819 1107 52-53 0.018315138836406675 1107 54-55 -0.07323266410752893 1107 56-57 -0.15433173522900745 1107 58-59 -0.13997810603221694 1107 60-61 -0.16584548607068683 1107 62-63 -0.39062619722483305 1107 64-65 -0.3516957983480822 1107 66-67 0.022364974248056058 1107 68-69 0.15081173148649896 1107 70-71 0.07355973055513942 1107 72-73 -0.25181696491695504 1107 74-75 0.003198958574575528 1107 76-77 -0.1271275221526018 1107 78-79 0.1689722938335123 1107 80-81 0.2693129103287788 1107 82-83 0.1721079353136865 1107 84-85 0.09181368888286912 1107 86-87 0.14801587707019692 1107 88-89 0.153064010033944 1107 90-91 0.4093116087037032 1107 92-93 0.3193024776011626 1107 94-95 0.6164958739641904 1107 96-97 0.544310088955843 1107 98-99 0.8716519481401583 1107 100-101 1.3680903055304583 1108 1 -0.08664627638629696 1108 2 0.0018868278173655995 1108 3 -0.1403714781472445 1108 4 -0.14051655778286687 1108 5 -0.08033334441246609 1108 6 -0.14444579665633484 1108 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-0.9671157646136948 2316 62-63 -1.1426923129273092 2316 64-65 -0.9400031139281211 2316 66-67 -1.2938272510350757 2316 68-69 -1.5252672400470182 2316 70-71 -1.3490757148075758 2316 72-73 -1.2884713879350116 2316 74-75 -1.5288640962157523 2316 76-77 -1.5932279200711825 2316 78-79 -1.3930877000446635 2316 80-81 -1.568076131605718 2316 82-83 -1.6333710974380722 2316 84-85 -1.748177128380675 2316 86-87 -1.6414698896882989 2316 88-89 -2.2202907496844517 2316 90-91 -2.6387447120798875 2316 92-93 -3.0649950430599944 2316 94-95 -3.10503764302387 2316 96-97 -2.9563020934745197 2316 98-99 -3.2894285538494366 2316 100-101 -3.849464025660236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 10.0 5 34.0 6 109.0 7 181.0 8 222.0 9 416.0 10 705.0 11 1093.0 12 1522.0 13 2092.0 14 2497.0 15 3367.0 16 4557.0 17 6740.0 18 6274.0 19 3958.0 20 3342.0 21 3635.0 22 3835.0 23 4344.0 24 4954.0 25 5904.0 26 6848.0 27 8180.0 28 9984.0 29 12124.0 30 14386.0 31 17563.0 32 21101.0 33 27312.0 34 36831.0 35 55013.0 36 76657.0 37 130044.0 38 113552.0 39 8690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60780988995308 22.995693816051237 16.576373907715038 18.820122386280648 2 13.69138087570664 24.95966238460934 39.58336468381162 21.765592055872403 3 19.596473363797635 29.45975183797404 33.6861307156102 17.257644082618125 4 10.722030775301508 20.051431504639034 42.101602302044725 27.12493541801474 5 9.869514474797434 41.72744509876704 36.729234175035195 11.673806251400329 6 23.567032338645358 41.74111360242912 18.807778931475376 15.884075127450146 7 22.099328347353268 37.819210569876454 23.695276695141263 16.38618438762902 8 21.594042238708393 34.34139751236045 22.22506466558654 21.839495583344622 9 23.065090281017326 20.95917415316088 25.361951721935473 30.61378384388632 10-11 21.531090478316337 27.71766846075004 29.79950742128522 20.951733639648406 12-13 26.290093081660054 27.078954716533154 28.518568679283774 18.11238352252302 14-15 22.60631916524752 28.898982476116906 30.094830115897903 18.399868242737675 16-17 16.891977754814718 31.994843344001122 31.821533553377407 19.29164534780675 18-19 16.545277614755292 32.95863241689615 32.99784141506863 17.49824855327993 20-21 18.52980469070036 30.516053952919105 30.9170056698385 20.037135686542033 22-23 17.87779834369153 29.832446323801115 30.994671254704663 21.29508407780269 24-25 19.672884929532486 30.622060370153008 31.482484696786532 18.222570003527974 26-27 20.012138905191136 28.434800201311873 33.93166766152184 17.621393231975148 28-29 17.4925636665513 29.908439883158856 34.461365342589666 18.137631107700177 30-31 17.848955903671264 31.089809506133825 32.703648526861926 18.35758606333298 32-33 17.76861507799163 31.02844616997477 32.490465274538224 18.712473477495372 34-35 19.217492427656214 29.020699272402894 32.16609579735965 19.595712502581243 36-37 19.88512378038075 28.779084836171798 33.269290258931335 18.066501124516122 38-39 19.828296180727133 28.925103656900053 33.57563839189039 17.670961770482428 40-41 17.695632545229575 29.793582583687257 34.75035949570277 17.760425375380397 42-43 16.997104851526455 31.249963423983335 32.298169359915526 19.454762364574684 44-45 17.990833910185028 30.627731685376723 31.33140715028575 20.050027254152496 46-47 19.092505120595245 30.544329724532876 30.08502278142374 20.278142373448148 48-49 19.059785044041224 29.774978430834874 31.363405319725253 19.801831205398646 50-51 18.879352722238384 30.635506642990666 32.20086477593074 18.28427585884021 52-53 17.935734027558325 31.593590791821118 32.03517272859514 18.435502452025407 54-55 16.928255057459157 30.136587763782924 32.561442757370706 20.373714421387213 56-57 19.18799753209035 31.552124559212274 29.64041419415895 19.61946371453843 58-59 18.96927642854157 29.933285959118837 30.84521172093801 20.25222589140158 60-61 18.498605500414673 30.07186521843816 31.787484282618582 19.642044998528586 62-63 19.997759485818715 29.601455999812732 31.45439466824506 18.946389846123495 64-65 19.087321824185928 30.711532834510724 31.76206997450153 18.43907536680182 66-67 17.997140802916046 30.23801561690103 30.870383065527445 20.894460514655474 68-69 17.23675234254299 31.102734137255293 31.616973320759513 20.0435401994422 70-71 18.923901103035227 30.676652003002957 30.02146880752813 20.377978086433686 72-73 18.306422082775295 28.911661876313584 30.97335292947229 21.80856311143883 74-75 18.074762948583544 31.50137858503169 30.052150475607657 20.371707990777107 76-77 19.1703009249571 29.016866864772112 31.042397354873813 20.770434855396978 78-79 18.135708278365495 28.810671535604946 32.23263894113968 20.82098124488987 80-81 18.367367412557243 30.69663270782859 30.5501632732909 20.385836606323267 82-83 17.9558819349348 30.126973617109503 30.863835258670708 21.05330918928499 84-85 17.698640810464205 29.069584685583965 30.558607002108424 22.67316750184341 86-87 18.07682808909279 29.665588098636654 31.03998794459537 21.21759586767519 88-89 19.309329251962257 27.956020596035774 31.113430156753868 21.621219995248104 90-91 18.043739908997505 27.725147302426034 32.3305445471892 21.900568241387262 92-93 18.127180948272546 29.759211606532272 31.426304639703584 20.6873028054916 94-95 17.761007361928314 29.298568578962243 30.7558224108267 22.184601648282744 96-97 17.026237555887732 28.447733892461468 30.544547999077036 23.98148055257376 98-99 17.927123096189955 28.47116675612003 29.91880644131107 23.682903706378944 100-101 18.488771921317756 27.717524430403135 31.28604785508208 22.507655793197024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8322.0 1 4901.0 2 1309.5 3 1033.5 4 777.5 5 754.0 6 1271.0 7 2326.5 8 3168.5 9 3164.0 10 2327.5 11 1518.5 12 1326.5 13 1223.0 14 1134.0 15 1113.0 16 1122.0 17 1117.0 18 1131.5 19 1220.0 20 1364.5 21 1546.0 22 1753.5 23 2000.0 24 2189.5 25 2863.5 26 4496.0 27 5966.0 28 6262.0 29 6811.0 30 8536.0 31 10501.0 32 13187.5 33 16569.5 34 20193.0 35 22912.0 36 26517.5 37 30740.5 38 29831.0 39 26083.5 40 23181.5 41 20245.0 42 17438.5 43 16682.5 44 17105.5 45 17019.0 46 17749.5 47 24886.0 48 54950.5 49 52903.5 50 21677.0 51 13585.5 52 8328.0 53 4322.0 54 2559.5 55 1882.5 56 1495.0 57 1223.5 58 1088.0 59 942.5 60 731.0 61 552.5 62 400.0 63 244.5 64 140.0 65 80.0 66 57.5 67 49.0 68 32.0 69 20.0 70 18.5 71 15.0 72 9.5 73 9.0 74 9.0 75 6.0 76 4.0 77 3.5 78 2.0 79 2.0 80 1.0 81 0.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40496457813960407 2 0.0 3 0.0 4 0.0 5 5.016076525263469E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012540191313158673 16-17 0.0025080382626317347 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020900318855264456 36-37 0.007022507135368857 38-39 0.011787779834369152 40-41 0.002842443364315966 42-43 0.0012540191313158673 44-45 5.016076525263469E-4 46-47 3.344051016842313E-4 48-49 1.6720255084211566E-4 50-51 5.016076525263469E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.344051016842313E-4 60-61 0.0021736331609475035 62-63 0.0 64-65 3.344051016842313E-4 66-67 0.0011704178558948097 68-69 0.0015048229575790406 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12573631823327097 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13953052867774549 88-89 0.4222700421517631 90-91 0.3242893473582833 92-93 0.0 94-95 0.0 96-97 5.016076525263469E-4 98-99 0.0 100-101 8.360127542105783E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 598077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.51855713006757 #Duplication Level Percentage of deduplicated Percentage of total 1 73.81246948469938 16.621503110025724 2 11.16005737568446 5.026167791783649 3 4.147425440810058 2.8018211019473087 4 2.1974672722919624 1.9793516925024124 5 1.2748186862991693 1.4353538708952764 6 0.9447143221570932 1.2764162061052537 7 0.7304362093690729 1.1513858657383227 8 0.5272251541163819 0.9497879802702742 9 0.42010572100785526 0.8514157211265256 >10 3.710898792927138 17.839265386831187 >50 0.5881216599614058 9.234056269812886 >100 0.43644426598905844 18.210773830481905 >500 0.0193314325651031 2.8928420407206814 >1k 0.026023082299177245 12.424252602947035 >5k 0.0044610998227161 7.305606528811564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9801 1.6387522008035753 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8241 1.377916221489875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7441 1.2441541808161827 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6560 1.0968487335242785 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6039 1.0097362045355365 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5552 0.9283085622754261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4545 0.7599355935774157 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4334 0.7246558553497292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4161 0.6957298140540432 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3870 0.6470738717589876 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3254 0.5440771004402444 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3159 0.5281928581102433 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2939 0.4914082969249779 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2862 0.47853370051013494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2767 0.462649458180134 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2572 0.4300449607659214 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2437 0.4074726164022358 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2288 0.3825594363267606 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2141 0.3579806613529696 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2063 0.34493886238728455 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2037 0.3405915960653896 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2019 0.3375819501502315 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1987 0.3322314685232838 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1976 0.33039224046402055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1874 0.3133375802781247 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1808 0.3023022119225451 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1730 0.28926041295686006 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1632 0.2728745629743327 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1621 0.2710353349150694 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1564 0.26150478951706885 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1465 0.24495173698369943 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1440 0.24077167321264653 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1173 0.19612859213780165 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1165 0.19479097173106474 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1146 0.19161412326506455 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1075 0.1797427421552743 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1053 0.17606428603674779 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1039 0.17372345032495817 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1005 0.1680385635963262 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1004 0.1678713610454841 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1001 0.16736975339295776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.15650158758822025 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 884 0.14780705494443022 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 790 0.13209001516527136 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 789 0.13192281261442926 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 759 0.12690673608916578 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 752 0.12573631823327097 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 724 0.12105464680969172 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 708 0.11837940599621788 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 699 0.11687458303863885 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 691 0.11553696263190191 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 691 0.11553696263190191 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 691 0.11553696263190191 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 655 0.10951767080158575 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 647 0.10818005039484882 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 634 0.10600641723390132 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 632 0.1056720121322171 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 604 0.10099034070863784 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.360127542105783E-5 0.0 12-13 0.0 0.0 0.0 1.6720255084211566E-4 0.0 14-15 0.0 0.0 0.0 2.508038262631735E-4 0.0 16-17 0.0 0.0 0.0 5.01607652526347E-4 0.0 18-19 0.0 0.0 0.0 6.688102033684626E-4 0.0 20-21 0.0 0.0 0.0 0.001421221682157983 0.0 22-23 0.0 0.0 0.0 0.002758842088894908 0.0 24-25 0.0 0.0 0.0 0.005350481626947701 0.0 26-27 0.0 0.0 0.0 0.010533760703053285 0.0 28-29 0.0 0.0 0.0 0.03034726297784399 0.0 30-31 0.0 0.0 0.0 0.07666236956111003 0.0 32-33 0.0 0.0 0.0 0.15023149193164093 0.0 34-35 0.0 0.0 0.0 0.2614211882416478 0.0 36-37 0.0 0.0 0.0 0.41449512353760465 0.0 38-39 0.0 0.0 0.0 0.601260372828248 0.0 40-41 0.0 0.0 0.0 0.7982249777202601 0.0 42-43 0.0 0.0 0.0 0.9951895826122723 0.0 44-45 0.0 0.0 0.0 1.2282699384861817 0.0 46-47 0.0 0.0 0.0 1.5054917677824093 0.0 48-49 0.0 0.0 0.0 1.789903306764848 0.0 50-51 0.0 0.0 0.0 2.0694659717728654 0.0 52-53 0.0 0.0 0.0 2.3243662605316704 0.0 54-55 0.0 0.0 0.0 2.603761722988846 0.0 56-57 0.0 0.0 0.0 2.9433501037491827 0.0 58-59 0.0 0.0 0.0 3.3201410520718904 0.0 60-61 0.0 0.0 0.0 3.798591151306604 0.0 62-63 0.0 0.0 0.0 4.223870839373525 0.0 64-65 0.0 0.0 0.0 4.628751816237708 0.0 66-67 0.0 0.0 0.0 5.107452719298685 0.0 68-69 0.0 0.0 0.0 5.632552330218349 0.0 70-71 0.0 0.0 0.0 6.235735532381282 0.0 72-73 0.0 0.0 0.0 6.851291723306531 0.0 74-75 0.0 0.0 0.0 7.498867202718044 0.0 76-77 0.0 0.0 0.0 8.092519859482977 0.0 78-79 0.0 0.0 0.0 8.835149989048233 0.0 80-81 0.0 0.0 0.0 9.636886220336176 0.0 82-83 0.0 0.0 0.0 10.386288053210539 0.0 84-85 0.0 0.0 0.0 11.293779897906122 0.0 86-87 0.0 0.0 0.0 12.27124601012913 0.0 88-89 0.0 0.0 0.0 13.137438824766711 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGCGA 35 2.801221E-4 54.229244 6 GTATGGT 55 8.059378E-7 51.933617 1 CATTCCG 150 0.0 47.458523 9 ATGGGCG 40 5.413801E-4 47.45059 5 TAGGCAT 175 0.0 37.960476 5 CTCCGAC 45 5.7410944E-7 36.909103 94-95 GGCATTC 195 0.0 36.50045 7 CCTTTAG 185 0.0 36.026016 1 TTAGGCA 220 0.0 34.50952 4 GTAAGCG 35 2.0649594E-4 33.89611 94-95 CTTTAGG 200 0.0 33.215412 2 GGATATG 245 0.0 33.032608 1 GCCCCAT 75 3.0955338E-4 31.633726 6 TGCGCGC 30 0.0039711953 31.633726 26-27 GACATTC 60 0.003969622 31.633726 7 ATATGAG 260 0.0 29.200363 3 TCAGGGC 65 0.0058716456 29.200363 3 CTTTGCG 530 0.0 27.754118 2 CAGTGCA 70 0.008424225 27.119158 9 CGCGCCT 35 0.008432694 27.115755 28-29 >>END_MODULE