FastQCFastQC Report
Thu 26 May 2016
SRR938899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796590
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103660.37066570358901374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95370.3410224594953139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76630.2740122792400745No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59370.2122942583646512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.1707078978327177No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43500.15554657636621744No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40030.14313860809056744No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35130.12561726960333836No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31490.11260141815568245No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28480.10183831022781316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92700.023.2448141
GAGTACT83350.022.63393812-13
TACATGG93750.022.329692
ACATGGG94800.020.9832443
TTTGCGT8050.020.5922533
GTACTTT91550.020.52963414-15
CTTTGCG8050.020.0039022
AGTACTT89750.019.59574312-13
CATGGGA55300.017.72874
TTGCGTT9400.017.6348554
ACTTTTT110900.017.07734116-17
GGACGGT2304.3989887E-516.4664346
TACTTTT106350.016.24816914-15
AGAGTAC172200.016.13743610-11
GTATAGG7950.016.1193141
GTATCAA263500.016.0129411
TAGGACG2751.1604654E-515.5003514
CGCGGAA1854.8985385E-815.42240682-83
CATGGGG47000.015.4179014
GGACGCT8800.015.0630446
Produced by FastQC (version 0.11.5)