FastQCFastQC Report
Thu 26 May 2016
SRR938899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796590
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113170.40467140338769714No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94850.3391630521456488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79840.28549054384089195No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54300.19416503670541624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50630.18104191175681814No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41030.14671439145530807No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36050.12890699029889974No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35520.1270118251155872No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32240.11528325567923793No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29060.10391226457936273No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28730.10273225606899831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG6200.025.257552
TTTGCGT6550.024.6323913
GAGTACT90500.021.1595612-13
GTACATG86050.020.9261971
GTAAGCG2750.020.70849694-95
TACATGG85800.019.8552842
AGTACTT97200.019.21277612-13
GTACTTT99200.019.18440214-15
TTGCGTT8200.019.0971724
ACATGGG87650.018.7323883
GTGTAGC14100.017.5684571
TATCACG1650.001474144817.2558482
GGGCGTA3702.7848728E-916.6734739
CATGGGG45350.016.6375274
AGAGTAC175450.016.06722310-11
ACTTTTT119000.015.97143516-17
TACTTTT116300.015.79235214-15
AGTACAT68850.015.7833992
GTATAGC11150.015.3807331
GTATCAA283350.015.3327841
Produced by FastQC (version 0.11.5)