##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938899_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796590 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21269689157152 33.0 31.0 34.0 31.0 34.0 2 32.45341326401081 34.0 31.0 34.0 31.0 34.0 3 32.54262941653943 34.0 31.0 34.0 31.0 34.0 4 36.078554596848306 37.0 35.0 37.0 35.0 37.0 5 36.0050057391323 37.0 35.0 37.0 35.0 37.0 6 36.04463829163374 37.0 35.0 37.0 35.0 37.0 7 35.983514565953534 37.0 35.0 37.0 35.0 37.0 8 35.95525300455197 37.0 35.0 37.0 35.0 37.0 9 37.6411576241065 39.0 37.0 39.0 35.0 39.0 10-11 37.55127280008868 39.0 37.0 39.0 35.0 39.0 12-13 37.464487465091416 39.0 37.0 39.0 34.0 39.0 14-15 38.78184056297133 40.0 38.0 41.0 34.5 41.0 16-17 38.88063713307993 40.0 38.0 41.0 35.0 41.0 18-19 38.9179014442589 40.0 38.0 41.0 35.0 41.0 20-21 38.89451599984267 40.0 38.0 41.0 35.0 41.0 22-23 38.82557185715461 40.0 38.0 41.0 34.0 41.0 24-25 38.74818064142402 40.0 38.0 41.0 34.0 41.0 26-27 38.59098491376999 40.0 38.0 41.0 34.0 41.0 28-29 38.4367626287729 40.0 38.0 41.0 34.0 41.0 30-31 38.25981498896871 40.0 38.0 41.0 34.0 41.0 32-33 38.169375918529354 40.0 38.0 41.0 33.5 41.0 34-35 38.07516457542936 40.0 38.0 41.0 33.0 41.0 36-37 37.95101909825895 40.0 37.5 41.0 33.0 41.0 38-39 37.81616736811617 40.0 37.0 41.0 33.0 41.0 40-41 37.66012590333227 40.0 37.0 41.0 33.0 41.0 42-43 37.48810640816137 40.0 37.0 41.0 32.0 41.0 44-45 37.254484032339384 40.0 37.0 41.0 31.5 41.0 46-47 36.96165043856983 40.0 36.5 41.0 31.0 41.0 48-49 36.64106608405237 39.5 36.0 41.0 30.0 41.0 50-51 36.38646101144609 39.0 36.0 41.0 30.0 41.0 52-53 36.130635881555754 39.0 35.0 40.5 29.5 41.0 54-55 35.84145012318574 39.0 35.0 40.0 28.5 41.0 56-57 35.704147193546426 39.0 35.0 40.0 28.0 41.0 58-59 35.42855370290246 38.0 35.0 40.0 28.0 41.0 60-61 35.057480717588206 38.0 34.0 40.0 27.0 41.0 62-63 34.644483817792384 37.5 34.0 40.0 26.0 41.0 64-65 34.23044439835657 37.0 33.5 39.5 26.0 41.0 66-67 33.768330001895166 36.5 33.0 39.0 25.5 40.5 68-69 33.270405922927566 36.0 32.5 39.0 24.5 40.0 70-71 32.72374516822273 35.0 32.0 38.0 22.5 40.0 72-73 32.196594066345085 35.0 31.0 37.5 21.5 39.5 74-75 31.654422171287173 35.0 31.0 37.0 20.0 39.0 76-77 29.73885678630046 33.0 28.5 35.0 18.0 37.5 78-79 30.699555887706097 34.0 30.0 36.0 18.0 38.0 80-81 30.66440879785739 34.0 30.5 35.5 18.0 37.0 82-83 30.23227287518013 34.0 30.0 35.0 15.0 37.0 84-85 29.756220253952137 34.0 30.0 35.0 7.5 36.5 86-87 29.048684469300113 34.0 29.0 35.0 3.5 36.0 88-89 28.336907090420834 33.0 28.0 35.0 2.0 35.5 90-91 27.730711330584747 33.0 26.5 35.0 2.0 35.0 92-93 27.30644087978574 33.0 26.0 34.5 2.0 35.0 94-95 26.732064407009965 32.0 25.5 34.0 2.0 35.0 96-97 25.84719390400452 32.0 24.5 34.0 2.0 35.0 98-99 24.58349811735006 32.0 11.0 34.0 2.0 35.0 100-101 18.86913991682728 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.160017350250925 1101 2 0.16385948672927242 1101 3 0.19330340869610296 1101 4 0.1778767483855006 1101 5 0.11443410004592636 1101 6 0.1548078335424634 1101 7 0.2085791500433274 1101 8 0.10051806653663675 1101 9 0.14681828052182055 1101 10-11 0.32714672976796777 1101 12-13 0.2958886899725428 1101 14-15 0.3547301709718269 1101 16-17 0.34753676799341804 1101 18-19 0.258614168795269 1101 20-21 0.30339541642904777 1101 22-23 0.35317305509948227 1101 24-25 0.31806942148956807 1101 26-27 0.22921499495694064 1101 28-29 0.23961549852533182 1101 30-31 0.28422949110289153 1101 32-33 0.41454881207531 1101 34-35 0.5103451645262282 1101 36-37 0.5545615721062518 1101 38-39 0.442120847412113 1101 40-41 0.43630022796018153 1101 42-43 0.4865084199622274 1101 44-45 0.5186702411464452 1101 46-47 0.48066322233616887 1101 48-49 0.6237945612912341 1101 50-51 0.3944887910220416 1101 52-53 0.37975364518992905 1101 54-55 0.5365463397508705 1101 56-57 0.5461241389875084 1101 58-59 0.5874728340467854 1101 60-61 0.7294794372615598 1101 62-63 0.7375675454374075 1101 64-65 0.5655013685518284 1101 66-67 0.5448407702317368 1101 68-69 0.5653930236347051 1101 70-71 0.7980530247193371 1101 72-73 0.7899172713485356 1101 74-75 0.8432152239087571 1101 76-77 0.39845828514214077 1101 78-79 0.6351835008027216 1101 80-81 0.8442945728145013 1101 82-83 0.7679400733378543 1101 84-85 0.8024115950617023 1101 86-87 0.8692069817516312 1101 88-89 0.8588653362667351 1101 90-91 0.9274563825262447 1101 92-93 0.9045736226043921 1101 94-95 0.9929732249503118 1101 96-97 1.0677754794065493 1101 98-99 1.2557283367097902 1101 100-101 1.6552455146193914 1102 1 0.11567676093578427 1102 2 0.1455757000750708 1102 3 0.13281367145646783 1102 4 0.11092183404397815 1102 5 0.11888552859701207 1102 6 0.10826600465056657 1102 7 0.1018980912648928 1102 8 0.16043613414495184 1102 9 0.18662912224972672 1102 10-11 0.2627527589097767 1102 12-13 0.21326781759341173 1102 14-15 0.23568175022772664 1102 16-17 0.23320981331047363 1102 18-19 0.2764307853654415 1102 20-21 0.1568022796685895 1102 22-23 0.23486844270157547 1102 24-25 0.2104817605350462 1102 26-27 0.1613137997609897 1102 28-29 0.20108683435142183 1102 30-31 0.18195157896973058 1102 32-33 0.2091546181265258 1102 34-35 0.30251959466625067 1102 36-37 0.2551996645867902 1102 38-39 0.33535531383994766 1102 40-41 0.3685332727788406 1102 42-43 0.3081716730264574 1102 44-45 0.2910742320946582 1102 46-47 0.34301373384253964 1102 48-49 0.23636163679908861 1102 50-51 0.21456282031098795 1102 52-53 0.2153903937870041 1102 54-55 0.3316759043563664 1102 56-57 0.3480802904137974 1102 58-59 0.24985571148817343 1102 60-61 0.4064155639342957 1102 62-63 0.43088021223618966 1102 64-65 0.32191121800127576 1102 66-67 0.18353836915995458 1102 68-69 0.15047723736498142 1102 70-71 0.1983258211954535 1102 72-73 0.2555487126442628 1102 74-75 0.3793421043072769 1102 76-77 0.13460345481558633 1102 78-79 0.3995763295083101 1102 80-81 0.462213449126196 1102 82-83 0.4522958161998467 1102 84-85 0.6325754301787718 1102 86-87 0.7912330388509545 1102 88-89 0.604874180384563 1102 90-91 0.6372598047121301 1102 92-93 0.686369890464988 1102 94-95 0.8516399234588086 1102 96-97 0.9406478864234629 1102 98-99 1.171243901431577 1102 100-101 1.067487402962552 1103 1 0.05656066691121708 1103 2 0.055854489510721805 1103 3 0.09298260322615448 1103 4 0.019635504080426358 1103 5 0.09005417529760251 1103 6 0.11245617159760712 1103 7 0.08480556934493677 1103 8 0.06616805703041706 1103 9 0.05375975508858488 1103 10-11 0.11046425852720887 1103 12-13 0.1299504909428819 1103 14-15 0.20315692146532882 1103 16-17 0.1848898030584465 1103 18-19 0.14703891242082534 1103 20-21 0.1461562857901626 1103 22-23 0.12832902746807662 1103 24-25 0.08730982783924901 1103 26-27 0.07959668128935249 1103 28-29 0.13050026909690615 1103 30-31 0.2552374375958024 1103 32-33 0.1980186163411375 1103 34-35 0.2158233565611951 1103 36-37 0.15397928699955798 1103 38-39 0.1690690056706714 1103 40-41 0.18264722951169432 1103 42-43 0.12426200709198554 1103 44-45 0.2321487653121821 1103 46-47 0.2053806471138273 1103 48-49 0.16629835204180665 1103 50-51 0.2827746089204908 1103 52-53 0.20066371286010565 1103 54-55 0.3607711136554812 1103 56-57 0.3138935756796215 1103 58-59 0.27853385104766204 1103 60-61 0.2290673545081816 1103 62-63 0.3120565276150131 1103 64-65 0.16311407589883942 1103 66-67 0.3011948251612324 1103 68-69 0.33205222811695023 1103 70-71 0.30235678824222134 1103 72-73 0.2709855236364831 1103 74-75 0.26849606806078796 1103 76-77 0.06695788142133807 1103 78-79 0.32909931679992255 1103 80-81 0.43075581327014234 1103 82-83 0.464606384010402 1103 84-85 0.5072555992209651 1103 86-87 0.5777826160275268 1103 88-89 0.5619313258404155 1103 90-91 0.4418364422393708 1103 92-93 0.5512400212974633 1103 94-95 0.7783957645419441 1103 96-97 0.6584502623479338 1103 98-99 1.0102745967764086 1103 100-101 1.1576505287378822 1104 1 -0.06511239031478055 1104 2 0.006640969893439319 1104 3 0.011698817659912208 1104 4 0.02581740964084389 1104 5 0.01718488106539695 1104 6 0.10979031622420621 1104 7 0.09670542929935522 1104 8 0.10013341152007627 1104 9 0.14556244336730373 1104 10-11 0.1469238523376788 1104 12-13 0.12117656648950259 1104 14-15 0.15509568105833438 1104 16-17 0.12814582266712193 1104 18-19 0.22531633457279554 1104 20-21 0.17570722235215186 1104 22-23 0.1911548398987577 1104 24-25 0.16857210763448904 1104 26-27 0.07234278473038813 1104 28-29 0.11667635949684296 1104 30-31 -0.05216361578553119 1104 32-33 0.12657744867526333 1104 34-35 0.2105264851634132 1104 36-37 0.11999859172658489 1104 38-39 0.21136325852577187 1104 40-41 0.2814478956744537 1104 42-43 0.19370457177274147 1104 44-45 0.3152983915705576 1104 46-47 0.3558122249262965 1104 48-49 0.3254776172670262 1104 50-51 0.27961569790391394 1104 52-53 0.30132061380395214 1104 54-55 0.3878259470631775 1104 56-57 0.4071410216542546 1104 58-59 0.38458956074384076 1104 60-61 0.3587923157691151 1104 62-63 0.337585168140599 1104 64-65 0.2605981587608639 1104 66-67 0.17970040920636166 1104 68-69 0.2751749119454132 1104 70-71 0.2846067850880445 1104 72-73 0.24411463625675367 1104 74-75 0.23058741540020833 1104 76-77 0.19647136926742093 1104 78-79 0.2625368049717487 1104 80-81 0.3474189434322419 1104 82-83 0.30635229705367806 1104 84-85 0.5313275821906878 1104 86-87 0.7462335926189247 1104 88-89 0.6530064022002371 1104 90-91 0.6228819151885503 1104 92-93 0.7176959791989113 1104 94-95 0.9842090038386644 1104 96-97 0.9031680148744314 1104 98-99 1.2341671642672516 1104 100-101 1.2741917355155827 1105 1 -0.0012340160466379757 1105 2 0.033329966008494694 1105 3 0.11936642587522783 1105 4 0.07931250545054525 1105 5 -0.0037928021358624164 1105 6 -0.011754561775674688 1105 7 0.035778150197494085 1105 8 0.11967767327475798 1105 9 0.09437925825189097 1105 10-11 0.1916868018412572 1105 12-13 0.1702573387897317 1105 14-15 0.16631433142471508 1105 16-17 0.1549680648141063 1105 18-19 0.11411501610260188 1105 20-21 0.1940708194795917 1105 22-23 0.1206962923003303 1105 24-25 -0.046454278195042775 1105 26-27 0.07192683338204375 1105 28-29 0.21042266018484668 1105 30-31 0.22903896576075056 1105 32-33 0.1607114429240184 1105 34-35 0.24426697173657175 1105 36-37 0.28720756455118845 1105 38-39 0.3222112838507343 1105 40-41 0.2298240499305919 1105 42-43 0.2967007100450232 1105 44-45 0.15096638607192858 1105 46-47 0.29357832758889657 1105 48-49 0.2613132808719172 1105 50-51 0.22364436406724053 1105 52-53 0.1250376003649336 1105 54-55 0.07302547060594833 1105 56-57 0.1768678772008343 1105 58-59 0.2133006538937181 1105 60-61 0.251328824615463 1105 62-63 0.2547699272867163 1105 64-65 0.12169271048495744 1105 66-67 0.2534517338786557 1105 68-69 0.3186265703983082 1105 70-71 0.42801364115571516 1105 72-73 0.3133829611969574 1105 74-75 0.3884819808312301 1105 76-77 0.11418182011762212 1105 78-79 0.3717043160491542 1105 80-81 0.37117709324666137 1105 82-83 0.24209386715355663 1105 84-85 0.31212447842190727 1105 86-87 0.33007168438848566 1105 88-89 0.5911951822329868 1105 90-91 0.37791404150068786 1105 92-93 0.7342623125003378 1105 94-95 0.7752831197845218 1105 96-97 0.9154459782746827 1105 98-99 0.9746496310152146 1105 100-101 0.9092387836840921 1106 1 -0.03998114547067644 1106 2 -0.019425340721483053 1106 3 -0.04528749115755204 1106 4 -0.10897338915663113 1106 5 -0.0606187767587798 1106 6 -0.014259292468388196 1106 7 0.021746496189706477 1106 8 -0.017037458365699365 1106 9 -0.048694627322113604 1106 10-11 0.003266225102443343 1106 12-13 0.011557580132972589 1106 14-15 -0.09995876412394722 1106 16-17 -0.22595645594197578 1106 18-19 -0.14318050628627077 1106 20-21 -0.06247907736707958 1106 22-23 -0.0934703321494581 1106 24-25 0.008365605390721953 1106 26-27 -0.04599663998363468 1106 28-29 -0.006340405114571013 1106 30-31 -0.07277174694660715 1106 32-33 -0.015570786571863948 1106 34-35 -0.17150328992705965 1106 36-37 -0.0735724358249854 1106 38-39 -0.06748432492143053 1106 40-41 0.02819652414294893 1106 42-43 -0.017930478519978976 1106 44-45 -0.053608632767783604 1106 46-47 -0.046058225608419434 1106 48-49 0.08228832733450275 1106 50-51 0.026794758976933508 1106 52-53 0.1045678384255524 1106 54-55 0.006990107553868086 1106 56-57 0.09983759211816334 1106 58-59 -0.09461091225353613 1106 60-61 -0.029558792412807122 1106 62-63 0.10870715552844956 1106 64-65 0.08140428753309692 1106 66-67 0.21892615477637634 1106 68-69 0.07642073244274172 1106 70-71 0.20283596302348883 1106 72-73 0.19042387257625393 1106 74-75 0.19405452610522644 1106 76-77 -0.02422315353836524 1106 78-79 0.08366941621572721 1106 80-81 0.18689816366894263 1106 82-83 0.1557982477001829 1106 84-85 0.377260656335217 1106 86-87 0.49617340001368504 1106 88-89 0.6040974833204551 1106 90-91 0.6983391016031035 1106 92-93 0.5710572923851984 1106 94-95 0.5925167899879291 1106 96-97 0.5796397656803762 1106 98-99 0.6189106688748964 1106 100-101 0.8969556634464517 1107 1 -0.04778520657210095 1107 2 0.009505707604304803 1107 3 0.031119586445782943 1107 4 0.028713815724948688 1107 5 -0.013302734100534508 1107 6 0.017394087094075417 1107 7 0.0871543590472399 1107 8 0.044584862514938095 1107 9 -7.91832390888203E-4 1107 10-11 0.03147228355869203 1107 12-13 0.07270041249675074 1107 14-15 0.05018329133510946 1107 16-17 0.058549849995266356 1107 18-19 0.04547004563389123 1107 20-21 0.0449062847460695 1107 22-23 0.019470726921561265 1107 24-25 0.07799931010039529 1107 26-27 0.07199227973095645 1107 28-29 0.0455580825674744 1107 30-31 0.14495388165738632 1107 32-33 0.10313625489981604 1107 34-35 0.10550270054835664 1107 36-37 0.15619366723898054 1107 38-39 0.12427835728387748 1107 40-41 0.06892227623828973 1107 42-43 0.1436659724704512 1107 44-45 0.11677644344334936 1107 46-47 0.08976017934384117 1107 48-49 0.17559863767757378 1107 50-51 0.07632745125951601 1107 52-53 0.07580950292742017 1107 54-55 0.021152020746036726 1107 56-57 0.03793758402456149 1107 58-59 0.08187331714626822 1107 60-61 0.0704425408090188 1107 62-63 0.1273219622900612 1107 64-65 0.12180380428731752 1107 66-67 0.12570486055735586 1107 68-69 0.21048850940037767 1107 70-71 0.1600115985696604 1107 72-73 0.21584026536010725 1107 74-75 0.03743408991544328 1107 76-77 -0.04662020288952107 1107 78-79 0.2150854284253505 1107 80-81 0.11513406474711374 1107 82-83 0.12466125592535349 1107 84-85 0.18361974737958775 1107 86-87 0.3112366788963534 1107 88-89 0.5433068100555509 1107 90-91 0.5078885655469279 1107 92-93 0.7035115342593805 1107 94-95 0.9957398335556 1107 96-97 1.1024071544280645 1107 98-99 0.9878493071382799 1107 100-101 0.9798424011347855 1108 1 0.051511953995031945 1108 2 0.030342950749407294 1108 3 -0.04389155094047936 1108 4 0.01513478490064557 1108 5 -0.008304535988912676 1108 6 0.006861448889239341 1108 7 -0.004656144074317581 1108 8 0.04852087316838549 1108 9 0.051010433526649024 1108 10-11 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-0.8112149114581442 2316 58-59 -0.9044184661256196 2316 60-61 -0.7717883920345088 2316 62-63 -0.7423845858465725 2316 64-65 -0.6623318832282479 2316 66-67 -0.7957147332415175 2316 68-69 -0.7852332025223916 2316 70-71 -0.6805562215913312 2316 72-73 -0.4952788680572411 2316 74-75 -0.7339986041708713 2316 76-77 -0.7529118668213108 2316 78-79 -0.773017232262017 2316 80-81 -0.7999426031256114 2316 82-83 -0.7378349265478192 2316 84-85 -0.9049402028447417 2316 86-87 -1.115686934222591 2316 88-89 -1.2387595202528736 2316 90-91 -1.483957739602431 2316 92-93 -1.3815844070383143 2316 94-95 -1.2139408720264235 2316 96-97 -1.0263502277059615 2316 98-99 -1.0717024840208964 2316 100-101 -0.553560294106962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 25.0 5 59.0 6 141.0 7 318.0 8 711.0 9 1437.0 10 2346.0 11 3583.0 12 5482.0 13 7441.0 14 9185.0 15 12323.0 16 16539.0 17 23274.0 18 23376.0 19 16827.0 20 16280.0 21 17521.0 22 19143.0 23 21330.0 24 24603.0 25 28822.0 26 34135.0 27 41374.0 28 49487.0 29 60332.0 30 72499.0 31 88273.0 32 109360.0 33 139814.0 34 186462.0 35 267445.0 36 419179.0 37 633438.0 38 421398.0 39 22623.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.09400942305677 19.911053926249718 15.156409531044249 22.83852711964927 2 15.093739160906674 21.359548593108034 40.019273472335954 23.527438773649337 3 19.162801840813277 25.013284035200012 30.742654447022982 25.08125967696373 4 12.908077337042611 17.64552544348653 37.578050411393875 31.868346808076982 5 13.341211976013645 37.79624471231035 34.74914091804662 14.113402393629384 6 30.8754232833558 36.99112132990535 17.559706642732756 14.573748744006092 7 27.259591144930074 33.425421674253286 21.491638030601553 17.823349150215083 8 25.15956933265155 35.60089251552784 20.08066967270855 19.158868479112062 9 25.45650238325961 17.63204474020146 21.23990288172381 35.671549994815116 10-11 24.411873031084287 26.846606045219357 27.703059797825208 21.038461125871148 12-13 25.795003915482784 24.37139516339542 28.01631629949331 21.81728462162848 14-15 22.40357767539422 25.804339574502933 26.86759057407759 24.92449217602526 16-17 21.53436137588539 28.963133360414687 27.548084900046916 21.954420363653007 18-19 21.40180148610088 28.145091504623505 29.31080104985375 21.142305959421865 20-21 22.312602133312357 26.942758859897232 29.12296761413006 21.621671392660346 22-23 21.928866941525214 26.905445560486164 29.138575908517154 22.027111589471463 24-25 22.22032109502066 27.026698441117414 29.114261261005158 21.63871920285677 26-27 22.08080592450197 26.87485953873459 29.459775916343816 21.58455862041962 28-29 21.51353970371059 27.57193582184017 29.395084728186827 21.51943974626241 30-31 21.889747871514952 27.07726195116195 29.111417833861953 21.921572343461143 32-33 21.731179758205528 27.252350183616475 29.133623448556996 21.882846609621005 34-35 22.33274056828555 27.019160705488755 28.94642494727252 21.701673778953175 36-37 22.184413072865095 26.75029109448486 29.28000097284841 21.785294859801635 38-39 21.925713817743443 27.14105984871974 29.379365418252263 21.553860915284556 40-41 22.01272199389433 27.12762438506131 29.169934240851774 21.68971938019258 42-43 21.764531605559302 27.24839755538355 28.73847571037435 22.248595128682798 44-45 22.031503997448272 27.15829666294062 28.577732292902567 22.232467046708532 46-47 22.297833188869724 27.492766091958952 28.227420766715582 21.981979952455738 48-49 22.31658226947337 27.48536652435778 28.163875617334387 22.03417558883447 50-51 21.821978026300428 27.698747447990314 28.46870836976933 22.01056615593993 52-53 22.265097851311776 27.826084624489113 27.867939168773397 22.040878355425715 54-55 21.801229354320796 27.379004430395586 28.211142856121207 22.608623359162408 56-57 22.241372528686725 27.675025656245644 27.887087488691588 22.19651432637605 58-59 22.516813939679896 27.385539430804982 27.936608626980934 22.16103800253418 60-61 22.156255939245064 27.393018496205947 28.482880581460023 21.967844983088963 62-63 22.090937421275754 27.411100715363702 28.423652075530935 22.07430978782961 64-65 21.930751919718755 27.673879613533515 28.194642827450856 22.200725639296873 66-67 21.925207597744905 27.559955001119253 27.859399237765302 22.65543816337054 68-69 21.672653011161973 27.62885868013396 28.13942482103098 22.55906348767309 70-71 22.102455224447652 27.457249236793057 27.902667089801408 22.537628448957882 72-73 22.005889315201728 27.114038883068307 28.219760494030226 22.660311307699736 74-75 21.74823624485534 27.897224834530626 27.72773270304192 22.626806217572117 76-77 21.98191104414149 27.24614079607154 27.895503530818154 22.876444628968816 78-79 21.803545915042292 27.32225859923107 28.05521430381098 22.818981181915657 80-81 21.8687987928123 27.44666034347279 27.936812162702157 22.747728701012754 82-83 21.856139415527377 27.3134473257516 28.099869501762768 22.73054375695825 84-85 21.718059704961586 27.265951151136118 27.967467464782942 23.048521679119354 86-87 21.737335444756162 27.254874135135953 28.084452856890017 22.923337563217864 88-89 21.70183598513628 27.09234983147854 27.833542567268964 23.372271616116215 90-91 21.629222709652684 26.85970995045584 28.070753579939524 23.440313759951955 92-93 21.78978684755363 27.170750807233095 27.964002588867153 23.075459756346124 94-95 21.607543988002394 26.920557342275753 27.95929853770242 23.512600132019433 96-97 21.251792361411574 26.840098119495526 28.161940077022372 23.74616944207052 98-99 21.333624163713665 26.922627199553745 27.762775380016375 23.980973256716215 100-101 21.42915377293249 25.90553526488444 28.793886671496377 23.871424290686686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1021.0 1 680.0 2 392.0 3 445.5 4 407.5 5 416.5 6 677.5 7 1411.5 8 2308.0 9 2776.0 10 2646.5 11 2423.5 12 2553.0 13 2780.0 14 3014.5 15 3246.0 16 3393.5 17 3669.0 18 4016.0 19 4361.5 20 4907.5 21 5758.5 22 7071.0 23 8508.0 24 10348.5 25 12883.0 26 16088.5 27 20029.0 28 24011.5 29 28176.0 30 33064.5 31 38560.5 32 44269.5 33 49573.0 34 55802.5 35 61876.0 36 68273.5 37 75452.5 38 79942.0 39 81069.5 40 82545.5 41 87049.5 42 91335.5 43 94891.0 44 98675.5 45 101334.5 46 103822.5 47 114922.5 48 140124.0 49 139299.5 50 116278.0 51 105727.5 52 98371.0 53 92841.5 54 86837.0 55 78739.0 56 70619.5 57 62571.5 58 55165.5 59 47902.0 60 39638.5 61 31145.5 62 24024.0 63 18695.5 64 14166.5 65 10288.5 66 7141.5 67 5236.0 68 3606.0 69 2150.5 70 1327.0 71 788.0 72 528.0 73 388.5 74 256.5 75 154.0 76 92.5 77 43.5 78 22.0 79 17.0 80 7.5 81 4.0 82 6.5 83 6.0 84 4.5 85 3.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39626831248055666 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.118247580088609E-4 16-17 0.002789111024497692 18-19 3.575783364740631E-4 20-21 0.0 22-23 0.0 24-25 1.2515241776592207E-4 26-27 1.7878916823703152E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01331979303365885 36-37 0.024190174462470366 38-39 0.011549780268112236 40-41 0.006186105221001291 42-43 0.005703374466761306 44-45 0.002002438684254753 46-47 0.0013587976786014397 48-49 0.0032539628619139734 50-51 6.97277756124423E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.475825916562671E-4 60-61 0.001555465763662174 62-63 0.0014481922627199554 64-65 7.687934234192356E-4 66-67 0.0027175953572028794 68-69 0.0030930526105006455 70-71 8.939458411851577E-5 72-73 0.0 74-75 0.0 76-77 0.09368552415620453 78-79 0.0037366936161539587 80-81 2.681837523555473E-4 82-83 1.7878916823703152E-5 84-85 1.7878916823703154E-4 86-87 0.09230884756077938 88-89 0.43719315309001316 90-91 0.3895279608380206 92-93 0.0 94-95 0.0011442506767170017 96-97 0.0 98-99 0.0 100-101 4.2909400376887566E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2796590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.43319016500275 #Duplication Level Percentage of deduplicated Percentage of total 1 71.86509589839378 36.24386047669852 2 14.824079464008715 14.952512372589274 3 5.47047122031318 8.276799460386878 4 2.629457468774086 5.304477142138811 5 1.447719571245242 3.6506558221103766 6 0.897238447863696 2.7150358358677007 7 0.6041583695089751 2.13287437574569 8 0.40038573950685746 1.6154184111923677 9 0.29799894247698727 1.3526133601420454 >10 1.4269694828796258 12.484312898243521 >50 0.0876395771739572 3.0226693194572887 >100 0.04345966995543693 3.893667808480817 >500 0.0030536581292034543 1.0263585931045645 >1k 0.0019174132438310224 1.9219555843186076 >5k 2.840612213082996E-4 1.0014669707007977 >10k+ 7.10153053270749E-5 0.40532156882272113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11317 0.40467140338769714 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9485 0.3391630521456488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7984 0.28549054384089195 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5430 0.19416503670541624 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5063 0.18104191175681814 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4103 0.14671439145530807 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3605 0.12890699029889974 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3552 0.1270118251155872 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3224 0.11528325567923793 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2906 0.10391226457936273 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2873 0.10273225606899831 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.5757833647406305E-5 2 3.5757833647406305E-5 0.0 0.0 0.0 3.5757833647406305E-5 3 3.5757833647406305E-5 0.0 0.0 0.0 3.5757833647406305E-5 4 3.5757833647406305E-5 0.0 0.0 0.0 3.5757833647406305E-5 5 3.5757833647406305E-5 0.0 0.0 0.0 3.5757833647406305E-5 6 3.5757833647406305E-5 0.0 0.0 0.0 3.5757833647406305E-5 7 1.4303133458962522E-4 0.0 0.0 0.0 3.5757833647406305E-5 8 1.4303133458962522E-4 0.0 0.0 0.0 3.5757833647406305E-5 9 1.4303133458962522E-4 0.0 0.0 0.0 3.5757833647406305E-5 10-11 1.4303133458962522E-4 0.0 0.0 0.0 3.5757833647406305E-5 12-13 1.4303133458962522E-4 0.0 0.0 3.5757833647406305E-5 3.5757833647406305E-5 14-15 1.4303133458962522E-4 0.0 0.0 3.5757833647406305E-5 3.5757833647406305E-5 16-17 1.4303133458962522E-4 0.0 0.0 7.151566729481261E-5 3.5757833647406305E-5 18-19 1.4303133458962522E-4 0.0 0.0 2.1454700188443783E-4 3.5757833647406305E-5 20-21 1.4303133458962522E-4 0.0 0.0 3.57578336474063E-4 3.5757833647406305E-5 22-23 1.4303133458962522E-4 0.0 0.0 5.542464215347977E-4 5.363675047110946E-5 24-25 1.7878916823703154E-4 0.0 0.0 7.687934234192356E-4 7.151566729481261E-5 26-27 1.7878916823703154E-4 0.0 0.0 0.0014839500963673615 7.151566729481261E-5 28-29 1.7878916823703154E-4 0.0 0.0 0.0039691195348621 7.151566729481261E-5 30-31 1.7878916823703154E-4 0.0 0.0 0.011406748933522611 7.151566729481261E-5 32-33 1.7878916823703154E-4 0.0 0.0 0.025048362470008117 7.151566729481261E-5 34-35 1.9666808506073468E-4 0.0 0.0 0.044250319138665305 7.151566729481261E-5 36-37 2.1454700188443783E-4 0.0 0.0 0.07573509166520656 7.151566729481261E-5 38-39 2.5030483553184415E-4 0.0 0.0 0.12288179532931177 7.151566729481261E-5 40-41 2.5030483553184415E-4 0.0 0.0 0.1729606413525043 7.151566729481261E-5 42-43 2.681837523555473E-4 0.0 0.0 0.2257749616497234 7.151566729481261E-5 44-45 2.8606266917925044E-4 0.0 0.0 0.29344666182743984 7.151566729481261E-5 46-47 3.575783364740631E-4 0.0 0.0 0.37459906529022846 7.151566729481261E-5 48-49 3.575783364740631E-4 0.0 0.0 0.4599351352897636 7.151566729481261E-5 50-51 3.575783364740631E-4 0.0 0.0 0.5532809600263178 7.151566729481261E-5 52-53 3.575783364740631E-4 0.0 0.0 0.6489331650331296 7.151566729481261E-5 54-55 3.575783364740631E-4 0.0 0.0 0.7620173139430522 7.151566729481261E-5 56-57 3.9333617012146936E-4 0.0 0.0 0.8996313367350952 7.151566729481261E-5 58-59 3.9333617012146936E-4 0.0 0.0 1.0508333363131528 7.151566729481261E-5 60-61 3.9333617012146936E-4 0.0 0.0 1.2141751204145048 7.151566729481261E-5 62-63 3.9333617012146936E-4 0.0 0.0 1.3839354356555664 7.151566729481261E-5 64-65 3.9333617012146936E-4 0.0 0.0 1.5636006708169594 7.151566729481261E-5 66-67 3.9333617012146936E-4 0.0 0.0 1.7701021601307305 7.151566729481261E-5 68-69 4.2909400376887566E-4 0.0 0.0 2.010984806496483 7.151566729481261E-5 70-71 5.006096710636883E-4 0.0 0.0 2.2772555147518942 1.0727350094221891E-4 72-73 5.542464215347977E-4 0.0 0.0 2.5497659649787776 1.0727350094221891E-4 74-75 5.721253383585009E-4 0.0 0.0 2.836794095666508 1.0727350094221891E-4 76-77 5.721253383585009E-4 0.0 0.0 3.153179407778759 1.0727350094221891E-4 78-79 5.721253383585009E-4 0.0 0.0 3.5198581129160873 1.0727350094221891E-4 80-81 6.257620888296103E-4 0.0 0.0 3.912997614952496 1.4303133458962522E-4 82-83 7.151566729481261E-4 0.0 0.0 4.315934763408293 1.7878916823703154E-4 84-85 7.330355897718293E-4 0.0 0.0 4.74604786543612 1.7878916823703154E-4 86-87 7.687934234192356E-4 0.0 0.0 5.195702623552254 1.7878916823703154E-4 88-89 7.866723402429387E-4 0.0 0.0 5.678790956128714 1.7878916823703154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 620 0.0 25.25755 2 TTTGCGT 655 0.0 24.632391 3 GAGTACT 9050 0.0 21.15956 12-13 GTACATG 8605 0.0 20.926197 1 GTAAGCG 275 0.0 20.708496 94-95 TACATGG 8580 0.0 19.855284 2 AGTACTT 9720 0.0 19.212776 12-13 GTACTTT 9920 0.0 19.184402 14-15 TTGCGTT 820 0.0 19.097172 4 ACATGGG 8765 0.0 18.732388 3 GTGTAGC 1410 0.0 17.568457 1 TATCACG 165 0.0014741448 17.255848 2 GGGCGTA 370 2.7848728E-9 16.673473 9 CATGGGG 4535 0.0 16.637527 4 AGAGTAC 17545 0.0 16.067223 10-11 ACTTTTT 11900 0.0 15.971435 16-17 TACTTTT 11630 0.0 15.792352 14-15 AGTACAT 6885 0.0 15.783399 2 GTATAGC 1115 0.0 15.380733 1 GTATCAA 28335 0.0 15.332784 1 >>END_MODULE