FastQCFastQC Report
Thu 26 May 2016
SRR938898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803303
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103530.3693143409756277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94300.33638889552788265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.2687900665750367No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56900.20297484788479875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47630.1699067136160451No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43630.15563783151518049No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38800.1384081563783865No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36110.12881233316555507No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30480.10872888160858817No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28460.10152309614765154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92650.023.899931
TACATGG93250.022.8895822
ACATGGG93550.021.6020133
GAGTACT84000.021.55055212-13
AGTACTT90200.019.38716112-13
GTACTTT93600.019.34075714-15
GTATACG1600.001205818417.781011
CATGGGG44050.016.9757544
CATGGGA59850.016.6853854
CTTTGCG7250.016.3196982
ACTTTTT113000.016.23045216-17
GTATAGG8200.016.190841
AGAGTAC171050.015.88148910-11
TACTTTT110650.015.84727914-15
GTATCAA264900.015.7695431
TTTGCGT6950.015.6622153
GTACGGG2751.1692298E-515.48804956
TACACCG2457.568211E-515.4540325
CGCGGAA1906.77901E-815.01942882-83
GTATTAT12050.014.9527721
Produced by FastQC (version 0.11.5)