##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938898_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2803303 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738744259896272 33.0 31.0 34.0 30.0 34.0 2 31.812049214801252 33.0 31.0 34.0 30.0 34.0 3 31.857365044021286 34.0 31.0 34.0 30.0 34.0 4 35.39017259283067 37.0 35.0 37.0 33.0 37.0 5 35.42861331793245 37.0 35.0 37.0 33.0 37.0 6 35.50721345498506 37.0 35.0 37.0 33.0 37.0 7 35.42015615151127 37.0 35.0 37.0 33.0 37.0 8 35.40032097850286 37.0 35.0 37.0 33.0 37.0 9 36.99851425265125 39.0 37.0 39.0 33.0 39.0 10-11 36.88869772550452 39.0 37.0 39.0 33.0 39.0 12-13 36.76488413846096 39.0 37.0 39.0 32.0 39.0 14-15 37.962334967001425 40.0 38.0 41.0 33.0 41.0 16-17 37.890702325078664 40.0 38.0 41.0 33.0 41.0 18-19 37.83466253915471 40.0 38.0 41.0 32.0 41.0 20-21 37.71237322544156 40.0 37.5 41.0 32.0 41.0 22-23 37.7961449404506 40.0 38.0 41.0 32.0 41.0 24-25 37.72990932482147 40.0 38.0 41.0 32.0 41.0 26-27 37.64486999799879 40.0 37.5 41.0 32.0 41.0 28-29 37.517321174343266 40.0 37.0 41.0 32.0 41.0 30-31 37.346926464959374 40.0 37.0 41.0 31.0 41.0 32-33 37.169744048360094 40.0 36.5 41.0 31.0 41.0 34-35 36.85793936652585 40.0 36.0 41.0 30.0 41.0 36-37 36.59421992556638 39.5 36.0 41.0 30.0 41.0 38-39 36.393008176426164 39.0 35.5 41.0 30.0 41.0 40-41 36.261151577264386 39.0 35.0 41.0 30.0 41.0 42-43 36.06438761703605 39.0 35.0 41.0 29.0 41.0 44-45 36.000705774580915 39.0 35.0 41.0 28.5 41.0 46-47 35.79493939827411 39.0 35.0 41.0 27.0 41.0 48-49 35.621498104200654 39.0 35.0 41.0 26.5 41.0 50-51 34.46513059772704 38.0 34.0 40.0 24.5 40.5 52-53 34.62159210046149 38.5 34.5 39.5 25.0 40.5 54-55 35.13208525799744 39.0 35.0 40.5 26.0 41.0 56-57 35.035631003855094 39.0 35.0 41.0 25.5 41.0 58-59 34.8360806876745 38.5 34.5 40.5 25.0 41.0 60-61 34.56393297478011 38.0 34.0 40.0 24.0 41.0 62-63 34.22083217547301 38.0 34.0 40.0 23.0 41.0 64-65 33.81446226112554 37.0 33.5 40.0 22.0 41.0 66-67 33.43087404394031 37.0 33.0 39.5 22.0 41.0 68-69 33.03742620758441 36.0 33.0 39.0 20.5 41.0 70-71 32.60895682700015 36.0 32.0 39.0 19.5 40.5 72-73 32.16419256141773 35.0 32.0 38.0 18.0 40.0 74-75 31.692784012288364 35.0 31.0 37.0 16.0 39.5 76-77 31.268256588745487 35.0 31.0 37.0 12.5 39.0 78-79 30.824941684862463 35.0 31.0 36.0 7.5 39.0 80-81 30.371120959810625 34.5 30.5 36.0 7.0 37.5 82-83 29.984065047552832 34.0 30.0 35.5 2.0 37.0 84-85 29.619303193411486 34.0 30.0 35.0 2.0 37.0 86-87 29.224613429229734 34.0 29.0 35.0 2.0 36.0 88-89 28.79538440903463 34.0 29.0 35.0 2.0 36.0 90-91 28.359456683776244 34.0 29.0 35.0 2.0 35.5 92-93 27.935422606832013 34.0 28.0 35.0 2.0 35.0 94-95 27.45529987304262 34.0 27.0 35.0 2.0 35.0 96-97 26.85613560146727 33.5 25.5 35.0 2.0 35.0 98-99 26.141916339403913 33.0 24.5 35.0 2.0 35.0 100-101 23.50581992028689 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23720660873195243 1101 2 0.35530832552146663 1101 3 0.3205409140288076 1101 4 0.3231440115679334 1101 5 0.15970164882416782 1101 6 0.1605381351132138 1101 7 0.2046830521388685 1101 8 0.2950108788105368 1101 9 0.3338748063137942 1101 10-11 0.46516773436888315 1101 12-13 0.49143890888631603 1101 14-15 0.18367959985420157 1101 16-17 0.5195914447705263 1101 18-19 0.4614426456608385 1101 20-21 0.6729800670120838 1101 22-23 0.50378241522926 1101 24-25 0.5981117283793012 1101 26-27 0.47503622216728303 1101 28-29 0.462336129226955 1101 30-31 0.5579349656147414 1101 32-33 0.595443777288537 1101 34-35 0.46270256057696457 1101 36-37 0.35335526343701673 1101 38-39 0.31697574811138196 1101 40-41 0.37866862650341204 1101 42-43 0.261004809572043 1101 44-45 0.5337875399461183 1101 46-47 0.38700047341648514 1101 48-49 0.45768875414121624 1101 50-51 0.4370570482444478 1101 52-53 0.389614776791376 1101 54-55 0.41184648062514384 1101 56-57 0.332794089129699 1101 58-59 0.2177044475658576 1101 60-61 0.38083438968644856 1101 62-63 0.42326954165692854 1101 64-65 0.3346875275399839 1101 66-67 0.30054116225850436 1101 68-69 0.22729022430014112 1101 70-71 0.22730182811321242 1101 72-73 0.23094718038372974 1101 74-75 0.16517967560958624 1101 76-77 0.12832950034971446 1101 78-79 0.24254012431774186 1101 80-81 -0.043654267979949424 1101 82-83 -0.1491908221024012 1101 84-85 -0.3572744101454255 1101 86-87 -0.24847765667616173 1101 88-89 -0.17764932678889878 1101 90-91 -0.17034338521554204 1101 92-93 -0.16915078390939797 1101 94-95 -0.3259563228883735 1101 96-97 -0.4446090848532229 1101 98-99 -0.49940677195909444 1101 100-101 -0.11437305092160344 1102 1 0.31530372648757776 1102 2 0.20457807179684906 1102 3 0.23015334189340209 1102 4 0.20051969737998832 1102 5 0.2737454711277323 1102 6 0.25458922441201537 1102 7 0.28534415046335226 1102 8 0.305596591263523 1102 9 0.30113169686111974 1102 10-11 0.27250393032060316 1102 12-13 0.32017769439185884 1102 14-15 0.48832472778135383 1102 16-17 0.46454777907835876 1102 18-19 0.4917719430647338 1102 20-21 0.5352128753715633 1102 22-23 0.4323116869749484 1102 24-25 0.44046635944618373 1102 26-27 0.4507924668696077 1102 28-29 0.5392192254766925 1102 30-31 0.5148755407948684 1102 32-33 0.5110717960581113 1102 34-35 0.439477345746063 1102 36-37 0.5539497495264953 1102 38-39 0.5469393310062358 1102 40-41 0.5521876788600437 1102 42-43 0.4281013787629817 1102 44-45 0.5012911005189054 1102 46-47 0.6355737662744119 1102 48-49 0.6084069717279661 1102 50-51 0.5607855010796428 1102 52-53 0.46279725625276313 1102 54-55 0.45713230774712343 1102 56-57 0.4515456697578131 1102 58-59 0.44884247431327395 1102 60-61 0.37317607836508415 1102 62-63 0.27074823981693186 1102 64-65 0.3728379781305051 1102 66-67 0.2566095422481567 1102 68-69 0.3493882760600684 1102 70-71 0.2759333475580945 1102 72-73 0.26128653150279746 1102 74-75 0.25297707087607435 1102 76-77 0.21914492178587963 1102 78-79 0.20858554218837355 1102 80-81 0.056419279902495845 1102 82-83 0.09096552874450126 1102 84-85 0.10690859239236161 1102 86-87 0.1736620542809426 1102 88-89 0.23339221544534539 1102 90-91 0.33079077297556125 1102 92-93 0.31810202567918466 1102 94-95 0.20058251149805173 1102 96-97 0.14957114512480274 1102 98-99 0.19076463114022602 1102 100-101 0.5835896982229123 1103 1 -0.001998908772701924 1103 2 0.18245302646321093 1103 3 0.25440486425922515 1103 4 0.15356096141632491 1103 5 0.25626800398388383 1103 6 0.18695514256267387 1103 7 0.2395000741580091 1103 8 0.1649983505782444 1103 9 0.21283943276380768 1103 10-11 0.26572213053038496 1103 12-13 0.28305532756250074 1103 14-15 0.35962912098884914 1103 16-17 0.3577459969066865 1103 18-19 0.4078244515164329 1103 20-21 0.44944387532928687 1103 22-23 0.36320404403499396 1103 24-25 0.46739429295956114 1103 26-27 0.3227899640484253 1103 28-29 0.27182984407512834 1103 30-31 0.277727582004907 1103 32-33 0.3564979587166519 1103 34-35 0.31720847471014935 1103 36-37 0.3222507573111102 1103 38-39 0.3128910135520684 1103 40-41 0.20903814017815847 1103 42-43 0.23175857319476734 1103 44-45 0.309765974558033 1103 46-47 0.33985894053348176 1103 48-49 0.3153179732429763 1103 50-51 0.22353320690828582 1103 52-53 0.17575083503169253 1103 54-55 0.27469149055144015 1103 56-57 0.4476945959908676 1103 58-59 0.39730697760806066 1103 60-61 0.4332539733719116 1103 62-63 0.3976124306497937 1103 64-65 0.34169706926984134 1103 66-67 0.3978057891885314 1103 68-69 0.28184203575490585 1103 70-71 0.39711316485047377 1103 72-73 0.3353411394999668 1103 74-75 0.2786618267610059 1103 76-77 0.3335037280506903 1103 78-79 0.43734390091481856 1103 80-81 0.35373437066116153 1103 82-83 0.31307144290147804 1103 84-85 0.24613771900181547 1103 86-87 0.23253326183216316 1103 88-89 0.3901798131961023 1103 90-91 0.18590587888846244 1103 92-93 0.07453709905652417 1103 94-95 0.14383219930822477 1103 96-97 0.11378879791222474 1103 98-99 -0.0806319014979664 1103 100-101 0.226947865805041 1104 1 0.16807899701096574 1104 2 0.23796637746299254 1104 3 0.22387043287906394 1104 4 0.27293752705897134 1104 5 0.2961513783439216 1104 6 0.30205643972004026 1104 7 0.2568962803395465 1104 8 0.31418700660217525 1104 9 0.42195680619197873 1104 10-11 0.35628367977826514 1104 12-13 0.34035745100715786 1104 14-15 0.35851428453623413 1104 16-17 0.2713676774814928 1104 18-19 0.30612504854379097 1104 20-21 0.36953246478940827 1104 22-23 0.2356736550260834 1104 24-25 0.3253686098162447 1104 26-27 0.3206389147039488 1104 28-29 0.2766634988868475 1104 30-31 0.3699437526792977 1104 32-33 0.3394969448392118 1104 34-35 0.2218620214072189 1104 36-37 0.2268993252075049 1104 38-39 0.24365767832401986 1104 40-41 0.3340001595354565 1104 42-43 0.3638873686832014 1104 44-45 0.38766982202307787 1104 46-47 0.3986681884344634 1104 48-49 0.3891578746516515 1104 50-51 0.35666232643366413 1104 52-53 0.4226732792853838 1104 54-55 0.3592934153834477 1104 56-57 0.4013142324913943 1104 58-59 0.41306197746492046 1104 60-61 0.27499532550629624 1104 62-63 0.28719936583424044 1104 64-65 0.28942443346372215 1104 66-67 0.3964469223525029 1104 68-69 0.3732019910120812 1104 70-71 0.25133166809298757 1104 72-73 0.27023025123627775 1104 74-75 0.3312427333093275 1104 76-77 0.2622624148477506 1104 78-79 0.1835219453165955 1104 80-81 0.08137488797514791 1104 82-83 -0.06368858042696957 1104 84-85 0.10628562810606468 1104 86-87 0.05630985640717867 1104 88-89 0.06303353865977357 1104 90-91 0.1560376386995017 1104 92-93 0.19945437835770363 1104 94-95 0.2321202345448583 1104 96-97 0.3070433666187746 1104 98-99 0.1756210226830568 1104 100-101 0.2934635365110374 1105 1 0.1549113789607297 1105 2 0.15940145936877315 1105 3 0.17663873867119761 1105 4 0.07430326706999324 1105 5 0.22545176786326238 1105 6 0.20534433890981774 1105 7 0.2119198489688756 1105 8 0.1312530660428024 1105 9 0.24558750096604598 1105 10-11 0.2805087044072465 1105 12-13 0.35950862553566054 1105 14-15 0.4453799099827407 1105 16-17 0.27763365356774017 1105 18-19 0.2525935342956842 1105 20-21 0.293601984637867 1105 22-23 0.3309032405372605 1105 24-25 0.3495815024794595 1105 26-27 0.2196695720040296 1105 28-29 0.31091958489635374 1105 30-31 0.2684216481421444 1105 32-33 0.2512195625216691 1105 34-35 0.3101377661140887 1105 36-37 0.34185277134764647 1105 38-39 0.14027408808369302 1105 40-41 0.11938991565292412 1105 42-43 0.24051476135181105 1105 44-45 0.20035854812436327 1105 46-47 0.34372136915885676 1105 48-49 0.38720939088126016 1105 50-51 0.2997609505814083 1105 52-53 0.3156154365563282 1105 54-55 0.2813203570813698 1105 56-57 0.17116804435094224 1105 58-59 0.3109452837845055 1105 60-61 0.46164814344533767 1105 62-63 0.37193555153056224 1105 64-65 0.3027466010215889 1105 66-67 0.2629618643899505 1105 68-69 0.2622505924311582 1105 70-71 0.19187261729853589 1105 72-73 0.3497010409699399 1105 74-75 0.35593421769286593 1105 76-77 0.2122541517372163 1105 78-79 0.09638693291374167 1105 80-81 0.06012177190908474 1105 82-83 0.11574019094606669 1105 84-85 0.12945297458586325 1105 86-87 0.07993198101870647 1105 88-89 0.17502325064362978 1105 90-91 0.19492393243035622 1105 92-93 0.28489016498194175 1105 94-95 0.3545899725230548 1105 96-97 0.26702473333862287 1105 98-99 0.286043404781573 1105 100-101 0.4837140486396585 1106 1 0.18973669257310988 1106 2 0.19755459664026276 1106 3 0.30686464421102144 1106 4 0.25090985577917024 1106 5 0.21746136126211724 1106 6 0.2632099304382436 1106 7 0.2560576507876817 1106 8 0.2623936326629277 1106 9 0.23376029531036124 1106 10-11 0.2990190479242685 1106 12-13 0.27163116770094575 1106 14-15 0.21064169868828486 1106 16-17 0.3583329435911011 1106 18-19 0.29015962836725606 1106 20-21 0.378941638305065 1106 22-23 0.36044823468284903 1106 24-25 0.3225580984189165 1106 26-27 0.2444903296594063 1106 28-29 0.33136801170331154 1106 30-31 0.260163675817914 1106 32-33 0.19224660921261716 1106 34-35 0.30231990937824804 1106 36-37 0.1598587034331942 1106 38-39 0.21904770559628872 1106 40-41 0.3619396448443908 1106 42-43 0.35351924284412917 1106 44-45 0.44614682343441103 1106 46-47 0.4316228478564881 1106 48-49 0.3191989215216111 1106 50-51 0.3297648766579968 1106 52-53 0.3009465628171881 1106 54-55 0.27314365534335394 1106 56-57 0.4250952552645302 1106 58-59 0.4779094310452052 1106 60-61 0.4307291559407247 1106 62-63 0.35395827099650745 1106 64-65 0.25060057712829575 1106 66-67 0.43944160959799206 1106 68-69 0.44632106377119385 1106 70-71 0.5192510897478115 1106 72-73 0.4024864201646139 1106 74-75 0.4754286025492611 1106 76-77 0.40685596990301676 1106 78-79 0.419272569852275 1106 80-81 0.46072717772421257 1106 82-83 0.4889036291642732 1106 84-85 0.5455747235288761 1106 86-87 0.7275219678419269 1106 88-89 0.7196386672868336 1106 90-91 0.6864563780771498 1106 92-93 0.6514824959744878 1106 94-95 0.6884339938503778 1106 96-97 0.7394569231098451 1106 98-99 0.8172739295333393 1106 100-101 1.466914390910265 1107 1 -0.020831875531033717 1107 2 -0.05530084747194053 1107 3 -0.04224935984039391 1107 4 0.15666330395740857 1107 5 -0.05644756043670185 1107 6 0.05025946620595789 1107 7 0.06753897463971015 1107 8 0.1174870362354099 1107 9 0.062222675416414575 1107 10-11 0.05469660056252934 1107 12-13 0.04109093027850719 1107 14-15 -0.07732342406887227 1107 16-17 0.014135253518901436 1107 18-19 0.04067884153782586 1107 20-21 0.02930476786816172 1107 22-23 0.037501295802961465 1107 24-25 0.11861235231370415 1107 26-27 0.1387863825433513 1107 28-29 -0.017830016291270567 1107 30-31 -0.003707124583208099 1107 32-33 0.03200016333408939 1107 34-35 -0.15071253299685594 1107 36-37 -0.13688372482223343 1107 38-39 -0.03682529404022006 1107 40-41 -0.16157153307996452 1107 42-43 -0.009190657185641271 1107 44-45 0.08806874327191139 1107 46-47 0.07224102661925258 1107 48-49 -0.043661673623631714 1107 50-51 -0.11047126715445899 1107 52-53 -0.15116344279049798 1107 54-55 -0.11479671688271509 1107 56-57 -0.01221922991529567 1107 58-59 -0.02968697273003329 1107 60-61 -0.2869851005478452 1107 62-63 -0.3078759534168327 1107 64-65 -0.22786417756745436 1107 66-67 -0.2419062623129875 1107 68-69 -0.2692108534542612 1107 70-71 -0.1198651610307877 1107 72-73 -0.12589038338192182 1107 74-75 -0.18147560832891685 1107 76-77 -0.16979121903943195 1107 78-79 0.057538534858132095 1107 80-81 0.15100305024893146 1107 82-83 0.19362796669941318 1107 84-85 0.15296691259005613 1107 86-87 0.29094223551298626 1107 88-89 0.18584094945019203 1107 90-91 0.10331422965798964 1107 92-93 -0.10420555965666622 1107 94-95 0.028459255831553065 1107 96-97 -0.010101494536645106 1107 98-99 0.143171296113362 1107 100-101 0.3026156163812139 1108 1 0.24737938666152814 1108 2 0.28659385199638976 1108 3 0.16772583982914924 1108 4 0.2811508223487209 1108 5 0.1884288211743268 1108 6 0.23553707127866375 1108 7 0.2095699721250739 1108 8 0.23235970232150294 1108 9 0.39192041906009933 1108 10-11 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#Quality Count 2 9450.0 3 2520.0 4 2485.0 5 2916.0 6 3413.0 7 3938.0 8 4935.0 9 5929.0 10 7577.0 11 9479.0 12 12208.0 13 17296.0 14 16619.0 15 15478.0 16 18756.0 17 24471.0 18 21513.0 19 16333.0 20 16719.0 21 17908.0 22 19489.0 23 21640.0 24 24579.0 25 28282.0 26 32970.0 27 38487.0 28 44896.0 29 53547.0 30 64026.0 31 77620.0 32 95420.0 33 121817.0 34 163466.0 35 232887.0 36 357844.0 37 565257.0 38 541387.0 39 89711.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05315072178403 19.30249962794638 14.672363856994036 22.97198579327556 2 14.71515377422454 20.807033065590986 40.850327421150965 23.627485739033506 3 19.140794600214804 24.87278198624346 30.560494789881403 25.425928623660337 4 12.501013283929122 17.060773109747846 38.13954285628581 32.29867075003723 5 13.347446353916132 37.56891792914278 34.807415478112496 14.276220238828591 6 30.891488477505536 36.59886124594603 17.881109164156808 14.628541112391627 7 27.306063570729755 33.31811073104197 21.644469517899353 17.731356180328923 8 25.03898036574955 35.38638852400894 20.328274127569966 19.246356982671546 9 25.614740196256964 17.71390130878443 21.354888606569844 35.31646988838876 10-11 24.40057190486128 26.67890833937473 27.864116718862775 21.056403036901216 12-13 25.852292760851725 24.285003492882392 28.064189770005342 21.79851397626054 14-15 22.561193672763046 25.626970119793995 26.962942331229193 24.84889387621376 16-17 21.648562108678764 28.789960467985924 27.57820354988346 21.983273873451854 18-19 21.462810725959823 28.04859062339089 29.352216673129895 21.13638197751939 20-21 22.39131936899627 26.888576988500372 29.0865165295402 21.633587112963152 22-23 21.971534611033018 26.830639792688082 29.13523845441766 22.062587141861236 24-25 22.294160498296336 26.909120068064446 29.122839790484655 21.67387964315456 26-27 22.094173326784837 26.78151538152431 29.438906996472742 21.68540429521811 28-29 21.522656424118086 27.449618261719312 29.404258981993152 21.62346633216945 30-31 21.86750118302515 27.078811796323247 29.10363300327676 21.950054017374846 32-33 21.708468525800804 27.194715900741183 29.131880888137506 21.964934685320504 34-35 22.351211791331252 26.932957572979632 28.9688851806228 21.746945455066317 36-37 22.166881483065627 26.736460466845312 29.2290184421553 21.867639607933757 38-39 21.955957817289214 27.098475774754554 29.327402492079845 21.618163915876384 40-41 22.00673177045276 27.09757265719882 29.17545225888716 21.720243313461264 42-43 21.746088116915264 27.19081595316502 28.74172244821822 22.3213734817015 44-45 22.011474219603983 27.08445422466672 28.5964039503694 22.3076676053599 46-47 22.263637505157227 27.38916892388828 28.26030100274454 22.086892568209947 48-49 22.293037514322243 27.388419265603037 28.222078845278137 22.096464374796582 50-51 21.876223877970205 27.63216177731436 28.489663495565022 22.00195084915042 52-53 22.209402212369692 27.750678537545266 27.970404854754932 22.06951439533011 54-55 21.790691662601876 27.267565590829186 28.32748475905375 22.614257987515188 56-57 22.23759791918756 27.5885018720748 27.953483375505904 22.220416833231734 58-59 22.53308881812789 27.313240333388087 27.989506840137125 22.164164008346898 60-61 22.200960471126628 27.227795266543914 28.59605347565403 21.97519078667543 62-63 22.103087243217782 27.333343019590096 28.484088260454115 22.079481476738007 64-65 21.94147141827703 27.579241753919565 28.32617271049073 22.15311411731268 66-67 21.91844959732306 27.50025886823347 27.953677570956366 22.627613963487107 68-69 21.722411633994483 27.5386012976511 28.29601907461691 22.442967993737504 70-71 22.11155221194662 27.399199127417127 28.00453734480267 22.484711315833582 72-73 22.051377568479168 27.052652488408636 28.341120124373333 22.554849818738866 74-75 21.811344074377583 27.819281879651044 27.93259450748662 22.436779538484757 76-77 22.095621715533145 27.220394677827127 27.973646761981243 22.71033684465849 78-79 21.996781126849537 27.323992873494486 28.115402324167754 22.563823675488226 80-81 22.031370924277358 27.531783888391182 27.96609120722728 22.47075398010419 82-83 22.077076889436043 27.319780602394488 28.14764295742978 22.455499550739688 84-85 21.93916872317539 27.289129219550457 28.031122828646122 22.740579228628036 86-87 22.021292759023307 27.353559140404098 28.121341209548252 22.503806891024347 88-89 22.034224577440355 27.235044239277816 27.842524747910208 22.888206435371615 90-91 21.970207388457137 27.071421919770135 28.113163908885657 22.845206782887075 92-93 22.261406678308955 27.32120798422621 28.002034170329104 22.415351167135732 94-95 22.11492652343095 27.07185621758288 27.956212827692756 22.85700443129342 96-97 21.845790724159915 27.0119146885262 28.198879713161784 22.9434148741521 98-99 22.007815816890677 27.12732302349877 27.719395018879194 23.14546614073136 100-101 22.202515739969638 26.491641768445284 28.258587240174336 23.04725525141074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3331.0 1 1914.0 2 561.5 3 619.5 4 559.5 5 565.5 6 893.5 7 1713.5 8 2781.5 9 3372.5 10 3217.5 11 3067.5 12 3331.0 13 3649.0 14 3937.5 15 4235.0 16 4511.0 17 4749.5 18 4944.0 19 5178.0 20 5555.5 21 6258.5 22 7445.5 23 8894.5 24 10600.0 25 12932.5 26 16225.5 27 20238.5 28 24123.5 29 28276.0 30 33130.5 31 38549.5 32 44094.0 33 49315.0 34 55862.0 35 61973.0 36 68019.5 37 75121.0 38 79811.5 39 81390.0 40 83168.0 41 86854.5 42 90405.5 43 94197.5 44 98109.5 45 100606.0 46 104196.5 47 115437.5 48 139565.5 49 138420.5 50 114350.5 51 103609.0 52 96496.5 53 91485.0 54 86204.0 55 78096.0 56 70307.5 57 62434.0 58 54920.5 59 47724.5 60 39618.5 61 31292.5 62 24472.5 63 19047.5 64 14339.0 65 10572.0 66 7419.5 67 5525.5 68 3953.0 69 2417.5 70 1549.5 71 1014.5 72 666.0 73 472.5 74 332.5 75 224.5 76 151.0 77 91.0 78 61.0 79 37.0 80 29.5 81 24.5 82 21.0 83 17.0 84 15.0 85 16.0 86 10.0 87 5.0 88 6.5 89 6.5 90 5.0 91 5.5 92 3.5 93 3.0 94 3.0 95 1.5 96 0.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2856273474540569 2 0.0883243802043518 3 0.09271206145036766 4 0.10708796016698874 5 0.1088715704295968 6 0.08372266572682296 7 0.09428163848146276 8 0.09039336810897716 9 0.09592255992306219 10-11 0.08873461056475165 12-13 0.09301527519501103 14-15 0.10239706517632949 16-17 0.09042904031422931 18-19 0.10148742394239937 20-21 0.10371693677065946 22-23 0.11668378337982017 24-25 0.12854479162616386 26-27 0.13120237091744988 28-29 0.12410360207226975 30-31 0.11693348881658529 32-33 0.11160049413138716 34-35 0.38322650102397066 36-37 0.5859873156772565 38-39 0.5690608542851058 40-41 0.5309272668705453 42-43 0.5580203067595618 44-45 0.5804759599657975 46-47 0.569078690387732 48-49 0.5905176857442809 50-51 0.5818671759706318 52-53 0.5859873156772565 54-55 0.5763379841565467 56-57 0.5476932033390611 58-59 0.5422531920381065 60-61 0.48993990303581164 62-63 0.565350944938881 64-65 0.5981158654629913 66-67 0.6112967453036651 68-69 0.61327655269516 70-71 0.6097093321699438 72-73 0.6012015112173033 74-75 0.5980266849498609 76-77 0.5822060619205274 78-79 0.55939368666177 80-81 0.5540071836686936 82-83 0.5687398044378363 84-85 0.5784961525743025 86-87 0.5680263603327932 88-89 0.581456945610232 90-91 0.5947448420666621 92-93 0.6031813186087982 94-95 0.60180793870659 96-97 0.5987401290549041 98-99 0.6029316131720331 100-101 0.5811002235577103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2803303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.19528231061514 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28156260797061 38.24951848486045 2 14.226679923778917 14.851311499288025 3 5.182188480140869 8.114573721233109 4 2.4396943421695347 5.093621397645974 5 1.3786546157810378 3.597963343976196 6 0.8559453398508936 2.6805785193583684 7 0.5379524510589812 1.965500603689763 8 0.39338584905854745 1.6426308358889534 9 0.2789656066319189 1.3104619733794514 >10 1.3015675600009178 11.873276685981086 >50 0.0791767158487607 2.8321177437695977 >100 0.03963953790488961 3.7116895305113773 >500 0.0023961782558478742 0.8532220860152062 >1k 0.0019169426046356784 2.0440238112537696 >5k 2.0538670763953695E-4 0.8095550679875205 >10k+ 6.846223587984564E-5 0.36995469516110346 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10353 0.3693143409756277 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9430 0.33638889552788265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7535 0.2687900665750367 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5690 0.20297484788479875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4763 0.1699067136160451 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4363 0.15563783151518049 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3880 0.1384081563783865 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3611 0.12881233316555507 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3048 0.10872888160858817 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2846 0.10152309614765154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.5672205252161465E-5 0.0 0.0 0.0 0.0 8 3.5672205252161465E-5 0.0 0.0 0.0 0.0 9 3.5672205252161465E-5 0.0 0.0 0.0 0.0 10-11 3.5672205252161465E-5 0.0 0.0 0.0 0.0 12-13 7.134441050432293E-5 0.0 0.0 0.0 0.0 14-15 7.134441050432293E-5 0.0 0.0 0.0 0.0 16-17 7.134441050432293E-5 0.0 0.0 1.070166157564844E-4 0.0 18-19 7.134441050432293E-5 0.0 0.0 1.7836102626080734E-4 0.0 20-21 7.134441050432293E-5 0.0 0.0 2.67541539391211E-4 0.0 22-23 7.134441050432293E-5 0.0 0.0 3.388859498955339E-4 0.0 24-25 1.0701661575648441E-4 0.0 0.0 5.172469761563413E-4 0.0 26-27 1.0701661575648441E-4 0.0 0.0 0.0011058383628170054 0.0 28-29 1.4268882100864586E-4 0.0 0.0 0.0033888594989553394 0.0 30-31 1.4268882100864586E-4 0.0 0.0 0.009988217470605212 0.0 32-33 1.4268882100864586E-4 0.0 0.0 0.024025230237330747 0.0 34-35 1.7836102626080734E-4 0.0 0.0 0.044429731641567105 0.0 36-37 2.1403323151296882E-4 0.0 0.0 0.07591045277659961 0.0 38-39 2.1403323151296882E-4 0.0 0.0 0.12228431960440952 0.0 40-41 2.4970543676513027E-4 0.0 0.0 0.17529321660912145 0.0 42-43 3.0321374464337245E-4 0.0 0.0 0.23131641495764105 1.7836102626080733E-5 44-45 3.2104984726945323E-4 0.0 0.0 0.2997000324260346 3.5672205252161465E-5 46-47 3.2104984726945323E-4 0.0 0.0 0.3839221090263878 3.5672205252161465E-5 48-49 3.2104984726945323E-4 0.0 0.0 0.47592072637171223 3.5672205252161465E-5 50-51 3.2104984726945323E-4 0.0 0.0 0.5694889207481317 3.5672205252161465E-5 52-53 3.2104984726945323E-4 0.0 0.0 0.6695851286856969 3.5672205252161465E-5 54-55 3.567220525216147E-4 0.0 0.0 0.7801332927621452 3.5672205252161465E-5 56-57 3.567220525216147E-4 0.0 0.0 0.9190230239114359 3.5672205252161465E-5 58-59 3.567220525216147E-4 0.0 0.0 1.0747143637344947 3.5672205252161465E-5 60-61 3.567220525216147E-4 0.0 0.0 1.2423023840091492 3.5672205252161465E-5 62-63 3.567220525216147E-4 0.0 0.0 1.413421952603768 3.5672205252161465E-5 64-65 3.567220525216147E-4 0.0 0.0 1.5934952447166788 3.5672205252161465E-5 66-67 4.2806646302593764E-4 0.0 0.0 1.8060124075064308 3.5672205252161465E-5 68-69 5.35083078782422E-4 0.0 0.0 2.050598882817876 3.5672205252161465E-5 70-71 5.35083078782422E-4 0.0 0.0 2.316856222820009 3.5672205252161465E-5 72-73 5.529191814085027E-4 0.0 0.0 2.591211153414383 7.134441050432293E-5 74-75 5.707552840345834E-4 0.0 0.0 2.880851623959308 7.134441050432293E-5 76-77 5.707552840345834E-4 0.0 0.0 3.198690972756067 7.134441050432293E-5 78-79 5.707552840345834E-4 0.0 0.0 3.5660255063401993 7.134441050432293E-5 80-81 6.06427489286745E-4 0.0 0.0 3.9656968939854162 7.134441050432293E-5 82-83 6.06427489286745E-4 0.0 0.0 4.367954516511416 7.134441050432293E-5 84-85 6.06427489286745E-4 0.0 0.0 4.7931315309119285 7.134441050432293E-5 86-87 6.06427489286745E-4 0.0 0.0 5.247363556490326 7.134441050432293E-5 88-89 6.06427489286745E-4 0.0 0.0 5.738801692146728 7.134441050432293E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9265 0.0 23.89993 1 TACATGG 9325 0.0 22.889582 2 ACATGGG 9355 0.0 21.602013 3 GAGTACT 8400 0.0 21.550552 12-13 AGTACTT 9020 0.0 19.387161 12-13 GTACTTT 9360 0.0 19.340757 14-15 GTATACG 160 0.0012058184 17.78101 1 CATGGGG 4405 0.0 16.975754 4 CATGGGA 5985 0.0 16.685385 4 CTTTGCG 725 0.0 16.319698 2 ACTTTTT 11300 0.0 16.230452 16-17 GTATAGG 820 0.0 16.19084 1 AGAGTAC 17105 0.0 15.881489 10-11 TACTTTT 11065 0.0 15.847279 14-15 GTATCAA 26490 0.0 15.769543 1 TTTGCGT 695 0.0 15.662215 3 GTACGGG 275 1.1692298E-5 15.4880495 6 TACACCG 245 7.568211E-5 15.454032 5 CGCGGAA 190 6.77901E-8 15.019428 82-83 GTATTAT 1205 0.0 14.952772 1 >>END_MODULE