FastQCFastQC Report
Thu 26 May 2016
SRR938898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803303
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111130.39642521696727034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94210.33606784568061315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81470.2906214561893595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56120.20019241587513015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50690.1808224084232065No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40890.14586364727608825No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35860.12792052803425102No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35450.1264579676189124No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31010.1106195084869527No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29680.10587510518841522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6450.024.277583
CTTTGCG7250.022.253182
GAGTACT87500.021.31256312-13
GTACATG93750.021.1445031
GCGTATC1150.0036565620.7179891
ATTCGCG1850.020.56309180-81
TACATGG95150.020.3471012
AGTACTT94050.020.00486412-13
TTGCGTT8650.019.748664
ACATGGG92300.019.6901153
GTACTTT97000.019.2986314-15
TACACCG2101.9746645E-518.0768135
TCGCGGA1751.2169039E-917.74564782-83
CGCGGAA1651.1288648E-817.37336282-83
TCGCGCT2156.91216E-1116.55289660-61
TACTTTT114500.016.36977814-15
CATGGGA56400.016.3220164
TATGTCG2053.3601528E-416.2029975
GTATAGG8000.016.0823381
ACTTTTT118850.015.79031416-17
Produced by FastQC (version 0.11.5)