##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938898_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2803303 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1887152405573 33.0 31.0 34.0 30.0 34.0 2 32.41889585249971 34.0 31.0 34.0 31.0 34.0 3 32.514298311670196 34.0 31.0 34.0 30.0 34.0 4 36.075627928911004 37.0 35.0 37.0 35.0 37.0 5 35.99282346574737 37.0 35.0 37.0 35.0 37.0 6 36.03292615889185 37.0 35.0 37.0 35.0 37.0 7 35.97802485139851 37.0 35.0 37.0 35.0 37.0 8 35.9455920391053 37.0 35.0 37.0 35.0 37.0 9 37.63358580931137 39.0 37.0 39.0 35.0 39.0 10-11 37.552511091380424 39.0 37.0 39.0 35.0 39.0 12-13 37.483603270855845 39.0 37.0 39.0 34.0 39.0 14-15 38.7454331551031 40.0 38.0 41.0 34.0 41.0 16-17 38.742331456856434 40.0 38.0 41.0 34.5 41.0 18-19 38.84668942315547 40.0 38.0 41.0 35.0 41.0 20-21 38.847318324134065 40.0 38.0 41.0 34.5 41.0 22-23 38.79193508514777 40.0 38.0 41.0 34.0 41.0 24-25 38.73188556499244 40.0 38.0 41.0 34.0 41.0 26-27 38.5812482632095 40.0 38.0 41.0 34.0 41.0 28-29 38.407470936962575 40.0 38.0 41.0 34.0 41.0 30-31 38.26032344701946 40.0 38.0 41.0 34.0 41.0 32-33 38.11442127376171 40.0 37.5 41.0 33.0 41.0 34-35 38.08687626703214 40.0 38.0 41.0 33.0 41.0 36-37 37.9909640520486 40.0 37.5 41.0 33.0 41.0 38-39 37.87547136360215 40.0 37.0 41.0 33.0 41.0 40-41 37.73525159428003 40.0 37.0 41.0 33.0 41.0 42-43 37.5802185850049 40.0 37.0 41.0 32.5 41.0 44-45 37.35103287086697 40.0 37.0 41.0 32.0 41.0 46-47 37.07036306813784 40.0 37.0 41.0 31.0 41.0 48-49 36.799368994361295 40.0 36.0 41.0 31.0 41.0 50-51 36.544622539910954 39.0 36.0 41.0 30.0 41.0 52-53 36.32910570138155 39.0 35.5 41.0 30.0 41.0 54-55 36.09097054438996 39.0 35.0 40.5 29.0 41.0 56-57 35.82453841058209 39.0 35.0 40.0 28.5 41.0 58-59 35.52900310811924 38.0 35.0 40.0 28.0 41.0 60-61 35.117200673633924 38.0 34.0 40.0 27.5 41.0 62-63 34.81544806251768 38.0 34.0 40.0 27.0 41.0 64-65 34.5575770796093 37.0 34.0 40.0 26.5 41.0 66-67 34.102697425144555 36.5 33.5 39.0 26.0 41.0 68-69 33.69064100455783 36.0 33.0 39.0 26.0 40.0 70-71 33.21868435199477 35.5 32.5 38.5 25.0 40.0 72-73 32.695185286784906 35.0 32.0 38.0 23.5 40.0 74-75 32.22265395499524 35.0 31.5 37.0 22.5 39.0 76-77 30.225111769937108 33.5 29.5 35.5 20.0 37.5 78-79 31.204818387452228 34.0 31.0 36.0 20.0 38.0 80-81 31.194978387994446 34.0 31.0 36.0 20.0 37.5 82-83 30.864583314754057 34.0 31.0 35.0 19.5 37.0 84-85 30.454029585813593 34.0 31.0 35.0 18.0 36.5 86-87 29.878759805843323 34.0 30.0 35.0 10.5 36.0 88-89 29.29550944011404 34.0 29.0 35.0 6.5 36.0 90-91 28.825687590674285 34.0 29.0 35.0 2.0 35.0 92-93 28.46625391547043 34.0 29.0 35.0 2.0 35.0 94-95 28.075510924077776 33.5 28.5 35.0 2.0 35.0 96-97 27.445774502435164 33.0 27.0 35.0 2.0 35.0 98-99 26.537925083374862 33.0 25.5 34.5 2.0 35.0 100-101 22.08742579735405 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10892814377885429 1101 2 0.10885473339082097 1101 3 0.15931469656514707 1101 4 0.07185316649417928 1101 5 0.18852320801439504 1101 6 0.04319317595902561 1101 7 0.07204132194193846 1101 8 0.10920525683057036 1101 9 0.1686029752712983 1101 10-11 0.2370987203486976 1101 12-13 0.18665695051296893 1101 14-15 0.1474236266332909 1101 16-17 0.1887401181379218 1101 18-19 0.24710360582025004 1101 20-21 0.2637663625544491 1101 22-23 0.28913008421391595 1101 24-25 0.301513665430754 1101 26-27 0.15391394638239575 1101 28-29 0.2535119841164928 1101 30-31 0.15716445939959556 1101 32-33 0.2656738893691539 1101 34-35 0.44117610248638783 1101 36-37 0.3554365267752857 1101 38-39 0.43890087362915153 1101 40-41 0.32508839266743195 1101 42-43 0.3730477144947173 1101 44-45 0.39931384392449587 1101 46-47 0.4950144618500545 1101 48-49 0.2798201473291613 1101 50-51 0.24513597138289356 1101 52-53 0.3237183390938938 1101 54-55 0.29832869321061395 1101 56-57 0.12510780090410378 1101 58-59 0.30215666058227697 1101 60-61 0.3254766452539499 1101 62-63 0.26045176359306765 1101 64-65 0.19715255765279238 1101 66-67 0.3043407653173702 1101 68-69 0.2647954393967282 1101 70-71 0.24275530440376514 1101 72-73 0.21388620537234004 1101 74-75 0.1816340924985198 1101 76-77 -0.05006301132945978 1101 78-79 0.23617380535115373 1101 80-81 0.3150172866882137 1101 82-83 0.2972464446967997 1101 84-85 0.21773480811127044 1101 86-87 0.4261579003103293 1101 88-89 0.5414572655770442 1101 90-91 0.6210215420241347 1101 92-93 0.7013906383538036 1101 94-95 0.6029888113638258 1101 96-97 0.5410810633951648 1101 98-99 0.35281481065007725 1101 100-101 0.9024033496922499 1102 1 0.19914282033384723 1102 2 0.1548916863164962 1102 3 0.16809470513487668 1102 4 0.10590226770620603 1102 5 0.07777020740523 1102 6 0.10123610560595608 1102 7 0.07844270646595675 1102 8 0.17159167462955338 1102 9 0.156084542280162 1102 10-11 0.20053349991604108 1102 12-13 0.21189786443249403 1102 14-15 0.22926596568817814 1102 16-17 0.1914105831264976 1102 18-19 0.21648749433106218 1102 20-21 0.2937699762278356 1102 22-23 0.32149858657569297 1102 24-25 0.24975168812836301 1102 26-27 0.2816436362493562 1102 28-29 0.28838931321536876 1102 30-31 0.20145128707862625 1102 32-33 0.18940095450845007 1102 34-35 0.21212259321587368 1102 36-37 0.22636459513374518 1102 38-39 0.1992911758438325 1102 40-41 0.12482539597496611 1102 42-43 -0.039355327334689605 1102 44-45 0.06105355295581916 1102 46-47 0.13681545889539137 1102 48-49 -0.05998105248970376 1102 50-51 -0.017371841486593098 1102 52-53 0.07423851265217252 1102 54-55 0.06807426058423971 1102 56-57 -0.031176422437894757 1102 58-59 -0.1619896671354084 1102 60-61 -0.07061523494088107 1102 62-63 0.016816473296998424 1102 64-65 -0.02077332975749613 1102 66-67 -0.14281612899656437 1102 68-69 -0.06173535201757119 1102 70-71 0.034058656708886303 1102 72-73 0.0479821342407476 1102 74-75 0.0909615470989138 1102 76-77 -0.029882585374789272 1102 78-79 -0.09079524308311449 1102 80-81 0.058046876046343954 1102 82-83 0.03739394544233221 1102 84-85 -6.198490103130894E-4 1102 86-87 0.17079211924853155 1102 88-89 0.2298440634347827 1102 90-91 0.41635940774218483 1102 92-93 0.41782645189618606 1102 94-95 0.39755516064461816 1102 96-97 0.2745247185521684 1102 98-99 0.5752477360857817 1102 100-101 1.0559966466113089 1103 1 0.04238592177247824 1103 2 0.05316071252285326 1103 3 0.04701054372579705 1103 4 0.03780853526666306 1103 5 -0.011999804035802697 1103 6 0.026935415697494136 1103 7 0.09539954326613298 1103 8 -0.002998941214322315 1103 9 0.06377919642100238 1103 10-11 0.026635429876407102 1103 12-13 -0.04910859732824946 1103 14-15 -7.117787949368903E-4 1103 16-17 0.02459683943430946 1103 18-19 0.07670934062775814 1103 20-21 0.04280026558304684 1103 22-23 0.0905561200660685 1103 24-25 0.11395689378991136 1103 26-27 0.07272658224962214 1103 28-29 0.12062773205082777 1103 30-31 0.14997851439900955 1103 32-33 0.17384218670135 1103 34-35 0.105400559165723 1103 36-37 0.08795855565431054 1103 38-39 0.1532576715857843 1103 40-41 0.21070742485090221 1103 42-43 0.18512698316445153 1103 44-45 0.33514519533872544 1103 46-47 0.28353016421177557 1103 48-49 0.23771073127691977 1103 50-51 0.1918199004872747 1103 52-53 0.3377053749005796 1103 54-55 0.3121495612304699 1103 56-57 0.27237276666394195 1103 58-59 0.18273369339879508 1103 60-61 0.14301114448183938 1103 62-63 0.3564228371273259 1103 64-65 0.21115046424926476 1103 66-67 0.2480453921231529 1103 68-69 0.31667960139013474 1103 70-71 0.2823168614509868 1103 72-73 0.23917766254101025 1103 74-75 0.2694462853457651 1103 76-77 0.07959791038362951 1103 78-79 0.18302750230911258 1103 80-81 0.27212411492775956 1103 82-83 0.2914488548497225 1103 84-85 0.27493760989498384 1103 86-87 0.10609657776157633 1103 88-89 0.40875231095341746 1103 90-91 0.5121681590484712 1103 92-93 0.41268377950663293 1103 94-95 0.22631975688818073 1103 96-97 0.18388166402747785 1103 98-99 0.5408821564634572 1103 100-101 1.0166498444272563 1104 1 0.01642285921813169 1104 2 0.04579927911507298 1104 3 0.07136503875992872 1104 4 0.044101047828114304 1104 5 0.06552689927760014 1104 6 0.06727227581960449 1104 7 0.044843833045057124 1104 8 0.11848365711664144 1104 9 0.14629147711693236 1104 10-11 0.09313187687976665 1104 12-13 0.10241511621534727 1104 14-15 0.14835717856260544 1104 16-17 0.12775357885646343 1104 18-19 0.07031185835194265 1104 20-21 0.10335492887298159 1104 22-23 0.1355622654274029 1104 24-25 0.11712375677345932 1104 26-27 0.15564442775107068 1104 28-29 0.12438990746586853 1104 30-31 0.0668589583199406 1104 32-33 0.16802936775557242 1104 34-35 0.18714472155649986 1104 36-37 0.18327976920378575 1104 38-39 0.3274901099382035 1104 40-41 0.2889889696676491 1104 42-43 0.2913866909614029 1104 44-45 0.36732684909439683 1104 46-47 0.36726646922527806 1104 48-49 0.18448873499083618 1104 50-51 -0.01532171887041045 1104 52-53 -0.09314599863297701 1104 54-55 0.15895214085774256 1104 56-57 0.09968977257364031 1104 58-59 0.11039075139505172 1104 60-61 0.2920829369897646 1104 62-63 0.1018939609823164 1104 64-65 0.18236462915848506 1104 66-67 0.2564049367675736 1104 68-69 0.2755251115560853 1104 70-71 0.07382163065799574 1104 72-73 0.0610654246074418 1104 74-75 0.11749204384322809 1104 76-77 -0.022633187011805944 1104 78-79 0.24452412702800075 1104 80-81 0.3151188939637457 1104 82-83 0.3995977409053033 1104 84-85 0.2998282941025181 1104 86-87 0.2516796831419015 1104 88-89 0.3621981734801558 1104 90-91 0.4584764958980827 1104 92-93 0.30886741814417107 1104 94-95 0.39449755871172343 1104 96-97 0.5783652496822818 1104 98-99 0.4981612114649572 1104 100-101 0.9343925198162282 1105 1 -0.007319766245700521 1105 2 0.02273339626346882 1105 3 -0.010391309608444033 1105 4 0.009260334806967307 1105 5 0.06168492816205173 1105 6 0.0826123209003029 1105 7 -0.06140380180160321 1105 8 0.07334878691298741 1105 9 0.03036187854580419 1105 10-11 0.013299789290520891 1105 12-13 -5.315731431423387E-4 1105 14-15 0.07251703993340186 1105 16-17 0.0489733149582392 1105 18-19 -0.020759014845268098 1105 20-21 0.030640101986854518 1105 22-23 -0.02718089075875696 1105 24-25 -0.03139006377362108 1105 26-27 0.023606676458008735 1105 28-29 -0.04073412994316783 1105 30-31 -0.03317947094393503 1105 32-33 -0.06875944692680491 1105 34-35 -0.02708232142763478 1105 36-37 -0.06508415537933132 1105 38-39 -0.10786268394161169 1105 40-41 -0.006156033252075588 1105 42-43 0.03548968903079697 1105 44-45 -0.04315391382474587 1105 46-47 -0.011461432071556032 1105 48-49 -0.047149180008716485 1105 50-51 -0.008948274583495675 1105 52-53 0.07606060219355015 1105 54-55 0.07157530794493994 1105 56-57 0.0294546294212239 1105 58-59 -0.03984453914232944 1105 60-61 -0.030947300536105615 1105 62-63 -0.24177180868309733 1105 64-65 -0.18710299316318668 1105 66-67 -0.034259426872608856 1105 68-69 -0.17273362383455293 1105 70-71 -0.10793937125405506 1105 72-73 -0.2240923366641283 1105 74-75 -0.11695280409029607 1105 76-77 -0.2142423018982491 1105 78-79 -0.08512601519166196 1105 80-81 -0.06969477801110102 1105 82-83 0.14032610100606746 1105 84-85 0.06865969102945968 1105 86-87 0.10814283437702343 1105 88-89 0.3446182863961411 1105 90-91 0.1861414991617032 1105 92-93 0.19503246009634623 1105 94-95 0.16339923643021237 1105 96-97 0.27532443865013434 1105 98-99 0.2876188607376058 1105 100-101 0.7823719607059196 1106 1 -0.0632550145400188 1106 2 -0.09578647552918085 1106 3 -0.10340022428447071 1106 4 -0.09082272399904667 1106 5 -0.005679713608145676 1106 6 0.012856268777163393 1106 7 -0.08715696928600636 1106 8 -0.02400216563433588 1106 9 -0.07333912256369501 1106 10-11 0.0034358888308432256 1106 12-13 0.04305007897444568 1106 14-15 0.014890589440270219 1106 16-17 -0.056633741623976164 1106 18-19 -0.07753646128341529 1106 20-21 -0.04119795842989049 1106 22-23 0.07284358383157752 1106 24-25 0.12803320064563906 1106 26-27 0.08494028007053345 1106 28-29 0.03465708317180116 1106 30-31 -0.004851867476077132 1106 32-33 0.17369166327744523 1106 34-35 0.12098541366922433 1106 36-37 0.17181146302844041 1106 38-39 0.24471153852307026 1106 40-41 0.27891015623570325 1106 42-43 0.18542945004456612 1106 44-45 0.14285530805142344 1106 46-47 0.25848789458361665 1106 48-49 0.06442939637082645 1106 50-51 0.2928239579929013 1106 52-53 0.2617904480890445 1106 54-55 0.24143810611390393 1106 56-57 0.25742976676050944 1106 58-59 0.3934754804053071 1106 60-61 0.4982184975984367 1106 62-63 0.43322765084749193 1106 64-65 0.44475666467830877 1106 66-67 0.563106538714031 1106 68-69 0.4208195635604781 1106 70-71 0.3064626173531195 1106 72-73 0.49176354136113076 1106 74-75 0.4928149995727793 1106 76-77 0.3239726890952639 1106 78-79 0.6566030679079766 1106 80-81 0.6507993951947988 1106 82-83 0.6353953518193727 1106 84-85 0.5581370184041994 1106 86-87 0.33247434043441615 1106 88-89 0.4994237400928583 1106 90-91 0.6620028672886349 1106 92-93 0.7567523992541716 1106 94-95 0.7285250670516383 1106 96-97 0.647544457477153 1106 98-99 0.7121721327394503 1106 100-101 1.2814749816497084 1107 1 -0.10717761752871269 1107 2 -0.07965844495907959 1107 3 -0.09946662511887183 1107 4 -0.11491841631067246 1107 5 -0.11795579436611092 1107 6 -0.0613770584065918 1107 7 -0.07525936159379398 1107 8 -0.062044919208545934 1107 9 -0.07088050892222242 1107 10-11 -0.07651745961462098 1107 12-13 -0.029663054150532275 1107 14-15 -0.09420719433690294 1107 16-17 -0.06943552000900155 1107 18-19 -0.004230137385732746 1107 20-21 -0.06549046769535494 1107 22-23 -0.10251255895798295 1107 24-25 -0.13199441765916475 1107 26-27 -0.18117131103242912 1107 28-29 -0.07772676742632001 1107 30-31 -0.1540651476429531 1107 32-33 -0.062109660282317236 1107 34-35 -0.15320252231914822 1107 36-37 -0.12659774453444328 1107 38-39 -0.12101637368286333 1107 40-41 -0.12186989648019164 1107 42-43 -0.17097956755114296 1107 44-45 -0.27233941743686785 1107 46-47 -0.17861881399760193 1107 48-49 -0.26028524187870516 1107 50-51 -0.36667175221948156 1107 52-53 -0.26735946706503455 1107 54-55 -0.25187970714470964 1107 56-57 -0.20482567333316126 1107 58-59 -0.17518897728407268 1107 60-61 -0.32634453223189297 1107 62-63 -0.3287946228038834 1107 64-65 -0.38419113636030033 1107 66-67 -0.32357484432007055 1107 68-69 -0.2734917414957536 1107 70-71 -0.2873021667510116 1107 72-73 -0.17776937684521243 1107 74-75 -0.30109207725250187 1107 76-77 -0.3732694076309109 1107 78-79 -0.1353440591124766 1107 80-81 0.021916712712151565 1107 82-83 -0.05234838611360004 1107 84-85 -0.05857360861534744 1107 86-87 -0.08238200602187362 1107 88-89 0.06297735010851113 1107 90-91 0.32066427454355306 1107 92-93 0.2768820701177752 1107 94-95 0.25030325114809315 1107 96-97 0.009389458874505152 1107 98-99 -0.1068868721613363 1107 100-101 0.2723150018885221 1108 1 0.061327426642385774 1108 2 0.00423696354638281 1108 3 0.10751659220633769 1108 4 0.06594580520700788 1108 5 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62.0 6 148.0 7 349.0 8 677.0 9 1368.0 10 2277.0 11 3528.0 12 5134.0 13 7236.0 14 8964.0 15 11678.0 16 15547.0 17 21826.0 18 21622.0 19 15370.0 20 14174.0 21 14974.0 22 16319.0 23 17907.0 24 21195.0 25 24921.0 26 30608.0 27 36762.0 28 45023.0 29 54692.0 30 66501.0 31 80961.0 32 100471.0 33 128195.0 34 171589.0 35 246170.0 36 391572.0 37 645872.0 38 535321.0 39 44255.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.992276836131886 20.044730020584836 15.156127042141424 22.806866101141853 2 15.106536824595842 21.331764707561046 40.0214318609155 23.540266606927613 3 19.161182362377524 25.030080587078885 30.72043942449318 25.088297626050416 4 12.897999253024024 17.690916750704435 37.60881360309607 31.80227039317548 5 13.30771107683395 37.833054851224915 34.761665927060044 14.09756814488109 6 30.925732965719366 36.974454777096874 17.539060172945987 14.560752084237771 7 27.29794103598505 33.40901786214334 21.482301413725168 17.810739688146448 8 25.156823932339815 35.62993368893766 20.07028851322886 19.14295386549367 9 25.471452782663878 17.647824726759826 21.204521951426585 35.67620053914971 10-11 24.43069835832944 26.860296585848907 27.724366577569388 20.98463847825226 12-13 25.793786829322414 24.3446391631586 28.071350118057165 21.790223889461824 14-15 22.417036234799905 25.794963885060028 26.89936973650791 24.888630143632156 16-17 21.590409866958094 29.00159798688256 27.50183762248661 21.906154523672733 18-19 21.439013192651668 28.152896779263603 29.292570228762283 21.115519799322442 20-21 22.322863422184472 26.96886494253386 29.117829931334573 21.590441703947093 22-23 21.89973755958596 26.93631405524126 29.103418360412697 22.060530024760077 24-25 22.245222867453144 27.02344341656967 29.094999719973192 21.636333996004 26-27 22.062010421278043 26.874404943026136 29.457821719592925 21.605762916102897 28-29 21.522557497352228 27.512509350576803 29.426822573228794 21.538110578842172 30-31 21.859267442727383 27.09605419036044 29.105683545446208 21.93899482146596 32-33 21.74008660497991 27.271115537635428 29.10455987098077 21.884237986403896 34-35 22.292482391753516 27.02947883015034 28.983901439481286 21.694137338614855 36-37 22.179111037541453 26.760451330818015 29.28928738591154 21.771150245728997 38-39 21.9491741753393 27.153843368295018 29.35657761863453 21.54040483773115 40-41 22.023355645178697 27.141863132730066 29.176327433509936 21.65845378858131 42-43 21.73277608174989 27.28753297028269 28.74969410854848 22.229996839418938 44-45 22.02906832914094 27.135833002026367 28.585539534507916 22.249559134324777 46-47 22.269664400345953 27.467653695445236 28.23337746591467 22.029304438294144 48-49 22.307031271459152 27.471746600691834 28.227554684832935 21.993667443016083 50-51 21.821505840469307 27.694419226015192 28.46890344720569 22.01517148630981 52-53 22.22239265609176 27.844902959116443 27.907061776768334 22.025642608023464 54-55 21.815033908214705 27.37451142455882 28.2179985538488 22.592456113377686 56-57 22.2143485738074 27.67367637390607 27.89684168996359 22.215133362322945 58-59 22.53279308842286 27.384099398690076 27.92638952033793 22.15671799254913 60-61 22.213369348891085 27.398168471903688 28.431118640512583 21.957343538692644 62-63 22.07611877845527 27.43171180568066 28.383410569603072 22.108758846261 64-65 21.911613701939842 27.681713297184224 28.186190793588906 22.220482207287027 66-67 21.941003152945786 27.564651263006546 27.837744847367425 22.656600736680243 68-69 21.706146175105836 27.59253330289245 28.184008012135926 22.51731250986579 70-71 22.062799144937088 27.445147286102728 27.91414768830692 22.577905880653265 72-73 21.997925304542534 27.161477014792908 28.188586820618394 22.65201086004617 74-75 21.78499791139238 27.848488015744284 27.771632249528505 22.59488182333483 76-77 21.93777862545934 27.252456297223347 27.952472209111136 22.857292868206173 78-79 21.836377571231637 27.34381228704797 28.0379937726998 22.7818163690206 80-81 21.858607506930216 27.458287598593518 27.906794235228944 22.776310659247322 82-83 21.83497467095066 27.318773603852314 28.121059336076048 22.725192389120977 84-85 21.692535555378782 27.268761172088784 27.94348309833079 23.095220174201646 86-87 21.707355543635085 27.292477081525835 28.06132957070408 22.938837804134998 88-89 21.712705004676096 27.134646462112876 27.818395508081938 23.334253025129083 90-91 21.609674966277108 26.96412013277611 28.04840704643316 23.377797854513624 92-93 21.84858361725436 27.173748253399648 27.956699650376716 23.020968478969273 94-95 21.590031473586695 26.90292487112524 27.994262482507242 23.512781172780823 96-97 21.260512430311756 26.857296350339965 28.203893391636285 23.67829782771199 98-99 21.377193260949674 26.949459262876687 27.738225229309855 23.935122246863788 100-101 21.4035090848448 26.210209771001626 28.499069844593595 23.887211299559983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1080.0 1 713.0 2 383.0 3 418.5 4 402.0 5 427.0 6 700.5 7 1445.5 8 2380.5 9 2898.0 10 2733.0 11 2458.5 12 2548.0 13 2764.0 14 3012.0 15 3186.0 16 3394.5 17 3692.5 18 3951.0 19 4288.5 20 4882.5 21 5843.0 22 7101.0 23 8518.5 24 10348.5 25 12796.0 26 16049.0 27 20246.0 28 24124.5 29 28149.5 30 33124.5 31 38654.0 32 44279.0 33 49600.0 34 56220.0 35 62220.5 36 68295.5 37 75513.0 38 80234.0 39 81798.5 40 83303.5 41 87348.0 42 91479.0 43 94922.5 44 98754.0 45 101757.0 46 104394.5 47 115422.5 48 141397.5 49 140324.5 50 115986.0 51 105704.5 52 98112.0 53 92468.5 54 86903.5 55 78305.0 56 70451.5 57 62848.5 58 55016.5 59 47617.5 60 39644.0 61 31247.5 62 24237.0 63 18845.0 64 14136.0 65 10309.0 66 7190.0 67 5273.0 68 3633.5 69 2158.5 70 1383.5 71 844.0 72 515.5 73 381.0 74 268.5 75 179.0 76 109.0 77 46.5 78 22.0 79 10.5 80 4.0 81 2.5 82 3.0 83 3.0 84 3.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4081613724952315 2 0.0 3 0.0 4 0.0 5 3.923942577737762E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011058383628170054 16-17 0.00315699016481629 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002532726572903464 36-37 0.007669524129214716 38-39 0.013466257482690954 40-41 0.0029786291385554826 42-43 7.491163102953908E-4 44-45 4.102303603998569E-4 46-47 2.67541539391211E-4 48-49 4.280664630259376E-4 50-51 6.420996945389065E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.3186933413904954E-4 60-61 0.0024970543676513025 62-63 0.0 64-65 2.3186933413904954E-4 66-67 0.0018371185704863157 68-69 0.001979807391494961 70-71 1.7836102626080736E-5 72-73 0.0 74-75 0.0 76-77 0.12519160433246068 78-79 5.35083078782422E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14969840934069562 88-89 0.44636630432029645 90-91 0.3416505458025765 92-93 0.0 94-95 0.0 96-97 5.172469761563414E-4 98-99 0.0 100-101 2.853776420172918E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2803303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.67166763348937 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90562483860171 36.43577922800003 2 15.005585873668775 15.207161200726546 3 5.360838482273924 8.149278774318116 4 2.5924345225555907 5.254519219540824 5 1.4253387554365036 3.611214584030494 6 0.894752309311285 2.72031549790311 7 0.5837850639634194 2.07069539113848 8 0.3961612154391807 1.6059319550410673 9 0.29418443815834877 1.341613446597419 >10 1.4060916186953825 12.381569416844709 >50 0.08683106037781238 2.992633650127689 >100 0.04336551995036206 3.9519966378041844 >500 0.0028204515874521805 0.9887354343466074 >1k 0.0018332935317984375 1.882180968632043 >5k 2.8204515873822116E-4 1.0093154802271604 >10k+ 7.051128968455529E-5 0.39705911472138605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11113 0.39642521696727034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9421 0.33606784568061315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8147 0.2906214561893595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5612 0.20019241587513015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5069 0.1808224084232065 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4089 0.14586364727608825 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3586 0.12792052803425102 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3545 0.1264579676189124 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3101 0.1106195084869527 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2968 0.10587510518841522 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7836102626080733E-5 0.0 12-13 0.0 0.0 0.0 3.5672205252161465E-5 0.0 14-15 0.0 0.0 0.0 3.5672205252161465E-5 0.0 16-17 0.0 0.0 0.0 1.0701661575648441E-4 0.0 18-19 0.0 0.0 0.0 1.7836102626080734E-4 0.0 20-21 0.0 0.0 0.0 2.67541539391211E-4 0.0 22-23 0.0 0.0 0.0 3.388859498955339E-4 0.0 24-25 0.0 0.0 0.0 5.172469761563413E-4 3.5672205252161465E-5 26-27 0.0 0.0 0.0 0.0011058383628170054 3.5672205252161465E-5 28-29 7.134441050432293E-5 0.0 0.0 0.0034602039094596623 3.5672205252161465E-5 30-31 7.134441050432293E-5 0.0 0.0 0.01020225070211818 3.5672205252161465E-5 32-33 7.134441050432293E-5 0.0 0.0 0.02427493567409588 3.5672205252161465E-5 34-35 7.134441050432293E-5 0.0 0.0 0.045053995233479936 3.5672205252161465E-5 36-37 1.4268882100864586E-4 0.0 0.0 0.07717681606305134 3.5672205252161465E-5 38-39 1.7836102626080734E-4 0.0 0.0 0.12437114361166096 3.5672205252161465E-5 40-41 1.7836102626080734E-4 0.0 0.0 0.17809348472141612 3.5672205252161465E-5 42-43 2.3186933413904954E-4 0.0 0.0 0.23422369968569218 3.5672205252161465E-5 44-45 2.853776420172917E-4 0.0 0.0 0.3031245641302421 3.5672205252161465E-5 46-47 2.853776420172917E-4 0.0 0.0 0.3880957570408907 3.5672205252161465E-5 48-49 3.2104984726945323E-4 0.0 0.0 0.4810040156201453 3.5672205252161465E-5 50-51 3.2104984726945323E-4 0.0 0.0 0.575517523435747 3.5672205252161465E-5 52-53 3.2104984726945323E-4 0.0 0.0 0.6770049473781464 3.5672205252161465E-5 54-55 3.567220525216147E-4 0.0 0.0 0.7876422919677252 3.5672205252161465E-5 56-57 3.567220525216147E-4 0.0 0.0 0.9264250065012594 3.5672205252161465E-5 58-59 3.9239425777377613E-4 0.0 0.0 1.0830616597635006 3.5672205252161465E-5 60-61 3.9239425777377613E-4 0.0 0.0 1.25130961583532 3.5672205252161465E-5 62-63 3.9239425777377613E-4 0.0 0.0 1.4238739087426513 3.5672205252161465E-5 64-65 3.9239425777377613E-4 0.0 0.0 1.6054097612709008 3.5672205252161465E-5 66-67 4.2806646302593764E-4 0.0 0.0 1.8185868598578177 5.35083078782422E-5 68-69 4.2806646302593764E-4 0.0 0.0 2.0642256652242015 7.134441050432293E-5 70-71 4.2806646302593764E-4 0.0 0.0 2.331678024102282 1.0701661575648441E-4 72-73 4.2806646302593764E-4 0.0 0.0 2.6087262061931944 1.4268882100864586E-4 74-75 4.2806646302593764E-4 0.0 0.0 2.900703206182136 1.4268882100864586E-4 76-77 4.2806646302593764E-4 0.0 0.0 3.2217173812463367 1.4268882100864586E-4 78-79 4.4590256565201836E-4 0.0 0.0 3.5914776247876166 1.4268882100864586E-4 80-81 4.994108735302605E-4 0.0 0.0 3.9923797035140334 1.7836102626080734E-4 82-83 4.994108735302605E-4 0.0 0.0 4.396777658355162 2.1403323151296882E-4 84-85 4.994108735302605E-4 0.0 0.0 4.823363724863135 2.1403323151296882E-4 86-87 5.529191814085027E-4 0.0 0.0 5.278737261009602 2.1403323151296882E-4 88-89 5.707552840345834E-4 0.0 0.0 5.771120710105187 2.1403323151296882E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 645 0.0 24.27758 3 CTTTGCG 725 0.0 22.25318 2 GAGTACT 8750 0.0 21.312563 12-13 GTACATG 9375 0.0 21.144503 1 GCGTATC 115 0.00365656 20.717989 1 ATTCGCG 185 0.0 20.563091 80-81 TACATGG 9515 0.0 20.347101 2 AGTACTT 9405 0.0 20.004864 12-13 TTGCGTT 865 0.0 19.74866 4 ACATGGG 9230 0.0 19.690115 3 GTACTTT 9700 0.0 19.29863 14-15 TACACCG 210 1.9746645E-5 18.076813 5 TCGCGGA 175 1.2169039E-9 17.745647 82-83 CGCGGAA 165 1.1288648E-8 17.373362 82-83 TCGCGCT 215 6.91216E-11 16.552896 60-61 TACTTTT 11450 0.0 16.369778 14-15 CATGGGA 5640 0.0 16.322016 4 TATGTCG 205 3.3601528E-4 16.202997 5 GTATAGG 800 0.0 16.082338 1 ACTTTTT 11885 0.0 15.790314 16-17 >>END_MODULE