FastQCFastQC Report
Thu 26 May 2016
SRR938897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2674656
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90920.3399315650311666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75930.2838869746240264No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71390.2669128291638252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.169891006544393No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42520.15897371475060718No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31220.11672529102807988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93250.021.9875491
GAGTACT79800.021.94670312-13
TACATGG96450.020.3764082
ACATGGG94500.019.894843
GTACTTT89000.019.7316114-15
GTATCAA210350.019.6524891
AGTACTT85650.018.84505712-13
GGTATCA167150.018.3145071
GTATAGG8100.018.1642971
TCAACGC234900.017.5395324
ATCAACG235850.017.4287223
CAACGCA235900.017.425035
TATCAAC237800.017.3853782
AGAGTAC153350.017.36205310-11
AACGCAG239500.017.1742046
ACTTTTT104700.017.11460316-17
CATGGGG46050.016.0426734
TACTTTT102650.015.86273514-15
ACGCAGA255200.015.7281467
CGCAGAG253900.015.4544738
Produced by FastQC (version 0.11.5)