##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938897_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2674656 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.655745262194465 33.0 31.0 34.0 30.0 34.0 2 31.749336363255686 33.0 31.0 34.0 30.0 34.0 3 31.791833043202566 34.0 31.0 34.0 30.0 34.0 4 35.32575703193233 37.0 35.0 37.0 33.0 37.0 5 35.36176166206047 37.0 35.0 37.0 33.0 37.0 6 35.440298490721794 37.0 35.0 37.0 33.0 37.0 7 35.360706573106974 37.0 35.0 37.0 33.0 37.0 8 35.33956142397378 37.0 35.0 37.0 33.0 37.0 9 36.92375729813479 39.0 37.0 39.0 33.0 39.0 10-11 36.80485864350406 39.0 37.0 39.0 32.5 39.0 12-13 36.68625124128113 39.0 37.0 39.0 32.0 39.0 14-15 37.85702254794636 40.0 38.0 41.0 32.0 41.0 16-17 37.76600972237178 40.0 38.0 41.0 32.0 41.0 18-19 37.6716831996339 40.0 37.5 41.0 32.0 41.0 20-21 37.79686453136404 40.0 38.0 41.0 32.0 41.0 22-23 37.73180644538961 40.0 38.0 41.0 32.0 41.0 24-25 37.64300306282378 40.0 37.5 41.0 32.0 41.0 26-27 37.5225116800067 40.0 37.0 41.0 32.0 41.0 28-29 37.38373775917351 40.0 37.0 41.0 31.0 41.0 30-31 37.19212564157783 40.0 37.0 41.0 31.0 41.0 32-33 36.99175520141655 40.0 36.5 41.0 30.5 41.0 34-35 36.66301311271431 39.5 36.0 41.0 30.0 41.0 36-37 36.39249757725853 39.0 35.5 41.0 30.0 41.0 38-39 36.1541377657538 39.0 35.0 41.0 29.5 41.0 40-41 36.01233037070936 39.0 35.0 41.0 29.0 41.0 42-43 35.807088649904884 39.0 35.0 41.0 27.5 41.0 44-45 35.73752755494539 39.0 35.0 41.0 27.0 41.0 46-47 35.52884800886544 39.0 35.0 41.0 26.0 41.0 48-49 35.29107331933527 39.0 35.0 41.0 25.0 41.0 50-51 34.19731472009859 38.0 34.0 40.0 23.5 40.5 52-53 34.309872746252225 38.0 33.5 39.5 24.0 40.5 54-55 34.80100188584999 39.0 34.0 40.0 24.0 41.0 56-57 34.70845596592609 38.5 34.0 40.0 23.5 41.0 58-59 34.49623016941244 38.0 34.0 40.0 23.0 41.0 60-61 34.17086253335008 38.0 34.0 40.0 22.5 41.0 62-63 33.790720189811324 37.5 33.5 40.0 22.0 41.0 64-65 33.40079191492289 37.0 33.0 40.0 20.5 41.0 66-67 32.97691572299391 36.5 32.5 39.0 18.5 41.0 68-69 32.53808826256535 36.0 32.0 39.0 17.5 41.0 70-71 32.08525507579292 35.0 31.5 39.0 13.5 40.0 72-73 31.631259870428195 35.0 31.0 38.0 10.0 40.0 74-75 31.15152322392113 35.0 31.0 37.0 7.5 39.0 76-77 30.70534528552457 35.0 30.5 37.0 6.5 39.0 78-79 30.284111115597668 34.5 30.0 36.0 2.0 39.0 80-81 29.812651982161444 34.0 29.5 36.0 2.0 37.5 82-83 29.322232279590345 34.0 29.0 35.0 2.0 37.0 84-85 28.88535254627137 34.0 29.0 35.0 2.0 36.5 86-87 28.420534266836558 34.0 28.5 35.0 2.0 36.0 88-89 27.918204621454123 34.0 27.0 35.0 2.0 36.0 90-91 27.440853889247812 33.5 26.0 35.0 2.0 35.0 92-93 26.917125230309992 33.0 25.5 35.0 2.0 35.0 94-95 26.358070533182584 33.0 25.0 35.0 2.0 35.0 96-97 25.672822972374767 32.5 22.0 35.0 2.0 35.0 98-99 24.769130123649546 32.0 10.0 35.0 2.0 35.0 100-101 21.46353418906955 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07584715490271776 1101 2 0.21291015588357354 1101 3 0.17821832013414962 1101 4 0.08481299480162363 1101 5 0.05653414786389277 1101 6 0.19481045262931218 1101 7 0.2259236628956529 1101 8 0.22497485342477574 1101 9 0.30041973234842345 1101 10-11 0.22437609324673957 1101 12-13 0.15536615390811903 1101 14-15 0.29068266178664715 1101 16-17 0.2876387662500406 1101 18-19 0.4461138689326347 1101 20-21 0.37788583934158027 1101 22-23 0.5050161388595384 1101 24-25 0.5005749663273349 1101 26-27 0.549724066893873 1101 28-29 0.49965998788949406 1101 30-31 0.4299008485471134 1101 32-33 0.36372990226361424 1101 34-35 0.3015195599548832 1101 36-37 0.28048313811132175 1101 38-39 0.6260448924908744 1101 40-41 0.37195100556331084 1101 42-43 0.41741590212604507 1101 44-45 0.44427455487022627 1101 46-47 0.4878474466180265 1101 48-49 0.37300751298401735 1101 50-51 0.4538187874158339 1101 52-53 0.42364097247897803 1101 54-55 0.5885382893155153 1101 56-57 0.704464832142925 1101 58-59 0.6736359762697006 1101 60-61 0.5723435149152039 1101 62-63 0.6530854461619526 1101 64-65 0.4254122197528787 1101 66-67 0.42079282666037443 1101 68-69 0.3789835531794239 1101 70-71 0.32097734635370045 1101 72-73 0.25528472918511724 1101 74-75 -0.0672382042784001 1101 76-77 -0.0673360284022948 1101 78-79 -0.0038530977207500428 1101 80-81 0.046719627516296214 1101 82-83 0.18169218883605254 1101 84-85 0.17282944555647362 1101 86-87 0.014589539190399847 1101 88-89 -0.028011360354600612 1101 90-91 -0.04236387904008865 1101 92-93 -0.02699684874773567 1101 94-95 -0.20406667088198205 1101 96-97 -0.35431920496679226 1101 98-99 -0.39136764075965047 1101 100-101 -0.1593636944516703 1102 1 0.2835863463435686 1102 2 0.3278777082820632 1102 3 0.45508844203189724 1102 4 0.3244692256180741 1102 5 0.41845644901270873 1102 6 0.45434741658616673 1102 7 0.5229244521616323 1102 8 0.5373246736475252 1102 9 0.6402513556054643 1102 10-11 0.6268337798204513 1102 12-13 0.5838312508124446 1102 14-15 0.6747888180613089 1102 16-17 0.7474344340567569 1102 18-19 0.6687723798508145 1102 20-21 0.7056197690092105 1102 22-23 0.6306815339310177 1102 24-25 0.6493812015285982 1102 26-27 0.7486717122503563 1102 28-29 0.7662290925110895 1102 30-31 0.6761671819681396 1102 32-33 0.7362809853119359 1102 34-35 0.7030794564733398 1102 36-37 0.7161316516603904 1102 38-39 0.9117273883253034 1102 40-41 0.7766504267682635 1102 42-43 0.7000345112639934 1102 44-45 0.8794385468021702 1102 46-47 0.8467288813281968 1102 48-49 0.7616563334698583 1102 50-51 0.7022632757083827 1102 52-53 0.8362385345240568 1102 54-55 0.9918849963224474 1102 56-57 1.0081272455429087 1102 58-59 0.9668252685612089 1102 60-61 0.968049688729117 1102 62-63 0.9219456145386999 1102 64-65 0.8426335650129033 1102 66-67 0.7759427871970743 1102 68-69 0.979810090055679 1102 70-71 0.8419477050312381 1102 72-73 0.7031493262209842 1102 74-75 0.6700513247928974 1102 76-77 0.7574644626965394 1102 78-79 0.683176631297929 1102 80-81 0.7384104106435458 1102 82-83 0.6077500244933347 1102 84-85 0.6861022684978053 1102 86-87 0.8053375879494986 1102 88-89 0.7117139312353302 1102 90-91 0.7256741372714082 1102 92-93 0.750188979543065 1102 94-95 0.7439678375807013 1102 96-97 0.6025605635151834 1102 98-99 0.49978929162656627 1102 100-101 0.5864301755825316 1103 1 0.25761186372351474 1103 2 0.34775162831421014 1103 3 0.40285315973332203 1103 4 0.3741891874424681 1103 5 0.2047107186530539 1103 6 0.31026589157109896 1103 7 0.2874814141869493 1103 8 0.2825225840353056 1103 9 0.4683179619948561 1103 10-11 0.42761864017808904 1103 12-13 0.35878478707927286 1103 14-15 0.4770153099238783 1103 16-17 0.5874836604983855 1103 18-19 0.6478147246368309 1103 20-21 0.6174975317176674 1103 22-23 0.5200777565550823 1103 24-25 0.5193913733188253 1103 26-27 0.5940586170122657 1103 28-29 0.528523145153244 1103 30-31 0.5930322613332208 1103 32-33 0.5469908758082198 1103 34-35 0.42888126981471686 1103 36-37 0.5361060338183137 1103 38-39 0.6582419804490698 1103 40-41 0.6391598450925571 1103 42-43 0.5954559498816323 1103 44-45 0.6354669022371766 1103 46-47 0.4116519022243139 1103 48-49 0.3505552685713269 1103 50-51 0.19634480072345184 1103 52-53 0.23974277904887487 1103 54-55 0.4056043553740878 1103 56-57 0.36631993190015777 1103 58-59 0.42827041624052953 1103 60-61 0.5254989553556015 1103 62-63 0.4704499846411707 1103 64-65 0.37323621218991576 1103 66-67 0.26021606929472085 1103 68-69 0.39287079429518457 1103 70-71 0.34827367951385924 1103 72-73 0.35868981245767273 1103 74-75 0.25975144534744743 1103 76-77 0.22176838574254987 1103 78-79 0.16906421417918338 1103 80-81 0.3019288454436264 1103 82-83 0.4069971864382822 1103 84-85 0.2877052672922602 1103 86-87 0.3162710066756418 1103 88-89 0.3754314066221127 1103 90-91 0.3025088268434395 1103 92-93 0.2767455146043467 1103 94-95 0.2464385779865701 1103 96-97 0.14186699811433812 1103 98-99 0.26517318252469835 1103 100-101 0.5437161912546209 1104 1 0.21280329408843457 1104 2 0.19672249857355695 1104 3 0.1862441165885862 1104 4 0.1717151550975018 1104 5 0.22418089940721586 1104 6 0.37894904169540666 1104 7 0.2941466309980285 1104 8 0.2145636923840044 1104 9 0.2881158430638564 1104 10-11 0.23263957864654827 1104 12-13 0.332995841157242 1104 14-15 0.3903623942313388 1104 16-17 0.41989744763652226 1104 18-19 0.47381830685105797 1104 20-21 0.3573576814070165 1104 22-23 0.43118132319983715 1104 24-25 0.3376286774029751 1104 26-27 0.2939256123265963 1104 28-29 0.2670956469943988 1104 30-31 0.31255100401186553 1104 32-33 0.4370306304778282 1104 34-35 0.34803779058142226 1104 36-37 0.36600986143707104 1104 38-39 0.49151772630091983 1104 40-41 0.2671998308328867 1104 42-43 0.36621184774590176 1104 44-45 0.3566118669957987 1104 46-47 0.5410690448307989 1104 48-49 0.540487689648927 1104 50-51 0.12173134993533807 1104 52-53 0.19864130377961686 1104 54-55 0.4155810197012144 1104 56-57 0.5162517845684604 1104 58-59 0.3770833057862433 1104 60-61 0.4588736631190784 1104 62-63 0.45332402884858425 1104 64-65 0.4525712443668297 1104 66-67 0.32140633380274863 1104 68-69 0.3843598690978496 1104 70-71 0.38978910502999753 1104 72-73 0.3605810090333783 1104 74-75 0.4158409623972581 1104 76-77 0.3091372963490677 1104 78-79 0.3263470818287857 1104 80-81 0.3988242494837806 1104 82-83 0.5778969320133385 1104 84-85 0.37906632643647953 1104 86-87 0.3947424583188379 1104 88-89 0.33930026903740895 1104 90-91 0.3490762216506518 1104 92-93 0.1623342229065834 1104 94-95 0.3309010326991775 1104 96-97 0.2628755446423483 1104 98-99 0.6138809317968885 1104 100-101 0.9300596636917149 1105 1 0.14115371437605262 1105 2 0.17024574076637577 1105 3 0.21850667754315722 1105 4 0.2497368520484926 1105 5 0.16279015148595022 1105 6 0.35423667461495967 1105 7 0.3372655374329483 1105 8 0.2626938135515502 1105 9 0.2623997498468782 1105 10-11 0.4056636063246941 1105 12-13 0.3839742925252523 1105 14-15 0.4284571458575499 1105 16-17 0.40841634620146294 1105 18-19 0.5227037198286695 1105 20-21 0.546880381781456 1105 22-23 0.5348297436024794 1105 24-25 0.4826730066227185 1105 26-27 0.5109917565471491 1105 28-29 0.47023887471854664 1105 30-31 0.4394469900153908 1105 32-33 0.5269048317497393 1105 34-35 0.567692782423876 1105 36-37 0.50400540581321 1105 38-39 0.6456098172658713 1105 40-41 0.6263320436010389 1105 42-43 0.538092835368829 1105 44-45 0.5746120425570638 1105 46-47 0.4981592984562866 1105 48-49 0.5369562596418689 1105 50-51 0.6425871959741656 1105 52-53 0.5440643004893602 1105 54-55 0.5366016814461076 1105 56-57 0.5606789251294728 1105 58-59 0.5049066639100417 1105 60-61 0.6230838341702309 1105 62-63 0.5976792800979425 1105 64-65 0.6298336388408856 1105 66-67 0.6542650463332862 1105 68-69 0.6605779010560511 1105 70-71 0.6057812229345245 1105 72-73 0.572160031278198 1105 74-75 0.43825453631006184 1105 76-77 0.5969808894222659 1105 78-79 0.6829551473555178 1105 80-81 0.702290071034831 1105 82-83 0.6624888580112369 1105 84-85 0.7563126782430984 1105 86-87 0.6305498433943129 1105 88-89 0.7545756883412764 1105 90-91 0.8239437939712992 1105 92-93 0.8640501829391525 1105 94-95 0.8549543639742048 1105 96-97 0.9395760673911511 1105 98-99 0.945013544118261 1105 100-101 0.9284429109092223 1106 1 0.12580210334805741 1106 2 0.1489100585397658 1106 3 0.1865580404029039 1106 4 0.18781385797679206 1106 5 0.16192023473001882 1106 6 0.2758892803815485 1106 7 0.30963933605961813 1106 8 0.2577307437014795 1106 9 0.30216155554383306 1106 10-11 0.30865246973773 1106 12-13 0.35016793457870676 1106 14-15 0.35605819105604297 1106 16-17 0.3862789123378505 1106 18-19 0.5087532560992969 1106 20-21 0.41835232047415616 1106 22-23 0.47478224933454527 1106 24-25 0.5310081252329866 1106 26-27 0.5033003851888935 1106 28-29 0.4394300852543367 1106 30-31 0.4019635517785929 1106 32-33 0.46864121274258963 1106 34-35 0.4610013874586514 1106 36-37 0.3764756979199291 1106 38-39 0.4241565428319234 1106 40-41 0.5050643789498181 1106 42-43 0.3804645388771206 1106 44-45 0.33476525177676564 1106 46-47 0.3263119352070234 1106 48-49 0.5053899816049068 1106 50-51 0.41569952382773323 1106 52-53 0.5156153962952814 1106 54-55 0.5489811014819352 1106 56-57 0.42129763424146205 1106 58-59 0.506393534009753 1106 60-61 0.5548548288793285 1106 62-63 0.6794036510460302 1106 64-65 0.488969387757173 1106 66-67 0.6894603792357898 1106 68-69 0.6755546777016832 1106 70-71 0.8709090104010002 1106 72-73 0.733463342450495 1106 74-75 0.7640284759883578 1106 76-77 0.6405941718825865 1106 78-79 0.7147072564905272 1106 80-81 0.7758334473623556 1106 82-83 0.763876800162361 1106 84-85 0.9191963283524274 1106 86-87 0.921865282646209 1106 88-89 0.9760802341579726 1106 90-91 1.0894434649491878 1106 92-93 1.1629191141041844 1106 94-95 1.093193446489412 1106 96-97 1.1588859029391152 1106 98-99 1.2789233300915903 1106 100-101 1.3533000887552618 1107 1 0.08238608129131109 1107 2 0.17231537582219758 1107 3 0.24200299279844373 1107 4 0.1877323803423252 1107 5 0.15497643335053368 1107 6 0.07216615698555273 1107 7 0.048351397133167495 1107 8 0.11203703170902912 1107 9 0.10787582474795698 1107 10-11 0.17598817276041956 1107 12-13 0.25375497298948346 1107 14-15 0.2873972485302758 1107 16-17 0.2599159858172513 1107 18-19 0.23789906278204853 1107 20-21 0.31912086837368747 1107 22-23 0.23935183000643434 1107 24-25 0.2666182576413547 1107 26-27 0.19479984092118485 1107 28-29 0.36701784887385003 1107 30-31 0.3306271379935595 1107 32-33 0.42200581287737293 1107 34-35 0.39715455963288804 1107 36-37 0.36361516119323056 1107 38-39 0.5369039643320477 1107 40-41 0.34381067796551434 1107 42-43 0.384897918992678 1107 44-45 0.28129165363630904 1107 46-47 0.4343911505369604 1107 48-49 0.432443734451752 1107 50-51 0.49134681234884425 1107 52-53 0.683783789185064 1107 54-55 0.6580610904159983 1107 56-57 0.5221347302572283 1107 58-59 0.4216560718502649 1107 60-61 0.36226936584727554 1107 62-63 0.2970732462365362 1107 64-65 0.47310327978053124 1107 66-67 0.32985306098400713 1107 68-69 0.47542242100956855 1107 70-71 0.39852205520141837 1107 72-73 0.4924629011133099 1107 74-75 0.5617552644332697 1107 76-77 0.44066828185860984 1107 78-79 0.5938615504268725 1107 80-81 0.5402642704670448 1107 82-83 0.6518266336316145 1107 84-85 0.6582808173366956 1107 86-87 0.8684335790341073 1107 88-89 0.7795955462469308 1107 90-91 0.8542738029441281 1107 92-93 0.7188329962895494 1107 94-95 0.7491525458726223 1107 96-97 0.9347553769244179 1107 98-99 0.7922657983582901 1107 100-101 0.9450967067111158 1108 1 0.3085727931330311 1108 2 0.3092874312922653 1108 3 0.23408474799405 1108 4 0.2427881232062319 1108 5 0.2086023193809794 1108 6 0.16594770404030612 1108 7 0.26986646963582217 1108 8 0.3231804800950684 1108 9 0.32578030904545585 1108 10-11 0.428821058714 1108 12-13 0.40297516069304606 1108 14-15 0.4657411044564128 1108 16-17 0.5232838736472587 1108 18-19 0.5133892337409023 1108 20-21 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#Quality Count 2 8958.0 3 2726.0 4 2859.0 5 3062.0 6 3624.0 7 4493.0 8 5518.0 9 6972.0 10 8503.0 11 10544.0 12 13395.0 13 18049.0 14 15704.0 15 16157.0 16 19664.0 17 25309.0 18 22705.0 19 17934.0 20 18036.0 21 19702.0 22 20979.0 23 23362.0 24 26461.0 25 30340.0 26 35067.0 27 40708.0 28 46913.0 29 55397.0 30 65895.0 31 79746.0 32 97578.0 33 123437.0 34 164373.0 35 230598.0 36 346678.0 37 520226.0 38 457004.0 39 65970.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07858754080108 19.297349228732514 14.761991043926177 22.86207218654023 2 14.810324206921225 20.60979461456364 40.87624483392984 23.7036363445853 3 19.121260865692445 24.890622625790527 30.324610339595694 25.66350616892133 4 12.731684476817238 17.19318614367666 37.86310381313508 32.212025566371025 5 13.525630443823319 37.49112387052773 34.56534197569822 14.417903709950735 6 30.980645373684652 36.436909664443995 17.99210342020519 14.590341541666168 7 27.584916855499653 33.123868077667126 21.474846399004562 17.81636866782866 8 25.228978189738577 35.41236276460346 20.29460201828533 19.06405702737263 9 25.874310581816196 17.61603288705252 21.238158019773234 35.271498511358054 10-11 24.456086524092242 26.78625040755281 27.746438273730707 21.01122479462425 12-13 25.794029357144677 24.352584275715934 28.00868458502992 21.84470178210947 14-15 22.66512661039538 25.621118099558092 26.724103907035467 24.98965138301106 16-17 21.770318818328434 28.808500624946927 27.31139640564798 22.109784151076664 18-19 21.646941168085494 27.794199324718054 29.250475950493293 21.308383556703163 20-21 22.520623317401633 26.750234354816655 29.117016900298136 21.612125427483576 22-23 22.21746564930058 26.732652889777086 29.069643649323407 21.980237811598926 24-25 22.306948145265114 26.84736235442053 29.10872803209485 21.736961468219516 26-27 22.100269664031398 26.88753595333308 29.269624363916964 21.74257001871856 28-29 21.668414862370284 27.333691579359794 29.258116386799433 21.739777171470497 30-31 21.952644621373064 26.93499697375351 29.134736725910003 21.977621678963423 32-33 21.819623334613567 26.990835639218236 29.157566130459177 22.031974895709023 34-35 22.405523199926776 26.901460307861864 28.921680705431275 21.771335786780085 36-37 22.28703565035023 26.701430783098335 29.09289031790357 21.918643248647864 38-39 22.02832239179221 27.068300690307108 29.148370754243082 21.755006163657605 40-41 22.17936938080795 26.949601852985744 29.091891234004063 21.77913753220224 42-43 21.89430946243509 27.121870843134456 28.69832335059333 22.28549634383713 44-45 22.1173394803635 27.06932394944183 28.566760640868484 22.24657592932619 46-47 22.347495942074676 27.293758846032944 28.24757562565515 22.11116958623723 48-49 22.365988701010888 27.25813941207898 28.165294555283882 22.21057733162625 50-51 21.989006567834394 27.4915668224674 28.356861292621815 22.16256531707639 52-53 22.381616789703088 27.594734285491473 27.90917008904285 22.114478835762586 54-55 21.995981671189618 27.254701281239235 28.218698099796075 22.530618947775068 56-57 22.245962944810586 27.562791069889837 27.978063772707234 22.213182212592347 58-59 22.57367327623626 27.26005602022032 27.949724923175516 22.216545780367905 60-61 22.33043429936925 27.163836848045914 28.50362310405657 22.002105748528265 62-63 22.191030234893073 27.26090972218047 28.45981807360023 22.088241969326223 64-65 22.032503839772353 27.445187519767334 28.21686290081764 22.305445739642668 66-67 22.04315686283354 27.423293143706857 27.912996149433845 22.62055384402576 68-69 21.844840040668632 27.382078171513125 28.252367308631783 22.520714479186463 70-71 22.195563018879415 27.274113061462092 28.01603461799366 22.51428930166483 72-73 22.163861222855886 27.04092630858848 28.273949638266423 22.521262830289206 74-75 21.949333682653386 27.630645918208362 27.896657897956572 22.52336250118168 76-77 22.10292893302872 27.21405169350813 27.95739412650124 22.72562524696191 78-79 22.114083526747514 27.246724482712953 28.019473230882525 22.61971875965701 80-81 22.17394849335492 27.424859371401155 27.86264819498295 22.53854394026098 82-83 22.148134843080012 27.27300910079186 28.058633013640105 22.520223042488023 84-85 22.05939161640644 27.294308851436412 27.890686357401872 22.755613174755275 86-87 22.11797221342808 27.225372978690825 28.0533711110965 22.6032836967846 88-89 22.161419906827962 27.10621210176733 27.841971686117752 22.89039630528696 90-91 22.151802392666397 27.06452982940131 28.01569553075775 22.76797224717454 92-93 22.27412843112528 27.221955142360667 27.93186970228092 22.57204672423313 94-95 22.15062198917376 27.03684609472895 27.919172888876442 22.893359027220846 96-97 22.031425541935366 26.946909822962585 28.050476017577076 22.97118861752498 98-99 22.11505282980571 27.044218415819998 27.70570922745571 23.135019526918583 100-101 22.337696208173355 26.28284792295265 28.367260888992273 23.012194979881723 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2286.0 1 1341.0 2 437.0 3 510.5 4 494.5 5 480.0 6 801.5 7 1631.5 8 2729.0 9 3365.5 10 3210.0 11 2990.5 12 3172.5 13 3444.0 14 3689.0 15 3948.0 16 4220.5 17 4391.0 18 4552.0 19 4809.0 20 5177.0 21 5873.0 22 6841.0 23 8217.0 24 9961.0 25 12466.0 26 15889.0 27 19769.5 28 23305.5 29 27166.0 30 31778.0 31 36890.0 32 41885.0 33 47032.5 34 53323.0 35 58718.0 36 64806.5 37 71631.5 38 76004.0 39 78260.5 40 80335.0 41 83179.0 42 86061.0 43 89928.0 44 94383.5 45 97086.5 46 98637.5 47 105439.0 48 121628.5 49 120923.5 50 105380.0 51 97233.5 52 91239.0 53 87118.0 54 82489.5 55 76093.5 56 69494.0 57 62547.0 58 55370.0 59 48343.5 60 40978.0 61 32809.5 62 25726.5 63 20365.0 64 15555.0 65 11615.0 66 8398.5 67 6252.5 68 4461.0 69 2872.5 70 2038.5 71 1420.5 72 936.0 73 629.0 74 424.5 75 283.0 76 181.0 77 109.5 78 82.0 79 54.5 80 36.0 81 29.0 82 21.0 83 14.5 84 13.0 85 14.5 86 11.0 87 8.5 88 10.5 89 11.0 90 8.5 91 4.5 92 2.5 93 2.0 94 2.0 95 2.5 96 3.0 97 3.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3357067226589139 2 0.09017982125551847 3 0.10147099290525585 4 0.10711657873012455 5 0.11833297440867162 6 0.05982077695225105 7 0.06202666810236531 8 0.06333524759819581 9 0.0654289747915246 10-11 0.0631670016630176 12-13 0.06146584831843796 14-15 0.05957775504588254 16-17 0.0609050285345106 18-19 0.06903691540145723 20-21 0.07075676273883445 22-23 0.08713270042951318 24-25 0.0963114508931242 26-27 0.09130146082337316 28-29 0.08887124175968798 30-31 0.08238442625892825 32-33 0.0734860856872809 34-35 0.33209878204898124 36-37 0.5148512556381082 38-39 0.48929656748381845 40-41 0.47011653087350297 42-43 0.500849455032722 44-45 0.5100468994891306 46-47 0.5049434394553917 48-49 0.5225157926851154 50-51 0.5191508739815512 52-53 0.518141398370482 54-55 0.5084579101013365 56-57 0.48722153428328724 58-59 0.4703969407654667 60-61 0.4288401947764498 62-63 0.4995034875512963 64-65 0.5240487000945169 66-67 0.5334517784716988 68-69 0.5466310433939916 70-71 0.5434904526039984 72-73 0.5310402534008112 74-75 0.5327601007381884 76-77 0.5072614945622914 78-79 0.4880440699663808 80-81 0.49400745366880827 82-83 0.5032048981252168 84-85 0.5049621334481893 86-87 0.5039339638443224 88-89 0.5108881291650216 90-91 0.5244038859576707 92-93 0.5307224555232524 94-95 0.5369101671392508 96-97 0.5288530562434944 98-99 0.5318814830767021 100-101 0.5100842874747257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2674656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.345415488247774 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41210903528672 41.73714507402988 2 13.51986305189111 14.965248759022462 3 4.806847689669841 7.981109477205036 4 2.157782146223474 4.776933976634448 5 1.181168203513002 3.2686122492467153 6 0.6779761370012288 2.2513722596070127 7 0.46838055060997413 1.81459013260863 8 0.30079867128396276 1.3318261952419026 9 0.2264514168049733 1.1279742975876261 >10 1.1322968923695511 11.180126954794753 >50 0.07551568325299142 2.8918946210634693 >100 0.03695006294759958 3.5598211638809554 >500 0.002505912077018869 0.9037388821219395 >1k 0.0011513650083290817 1.3165874946070069 >5k 2.0318206029336735E-4 0.8930184623481761 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9092 0.3399315650311666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7593 0.2838869746240264 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7139 0.2669128291638252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4544 0.169891006544393 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4252 0.15897371475060718 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3122 0.11672529102807988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.7387985595156907E-5 0.0 5 0.0 0.0 0.0 3.7387985595156907E-5 0.0 6 3.7387985595156907E-5 0.0 0.0 3.7387985595156907E-5 0.0 7 3.7387985595156907E-5 0.0 0.0 3.7387985595156907E-5 0.0 8 3.7387985595156907E-5 0.0 0.0 3.7387985595156907E-5 0.0 9 3.7387985595156907E-5 0.0 0.0 3.7387985595156907E-5 0.0 10-11 3.7387985595156907E-5 0.0 0.0 5.608197839273536E-5 0.0 12-13 7.477597119031381E-5 0.0 0.0 1.1216395678547073E-4 0.0 14-15 7.477597119031381E-5 0.0 0.0 1.1216395678547073E-4 1.8693992797578453E-5 16-17 7.477597119031381E-5 0.0 0.0 1.308579495830492E-4 7.477597119031381E-5 18-19 7.477597119031381E-5 0.0 0.0 2.617158991660984E-4 1.1216395678547073E-4 20-21 7.477597119031381E-5 0.0 0.0 4.11267841546726E-4 1.4955194238062763E-4 22-23 7.477597119031381E-5 0.0 0.0 6.16901762320089E-4 1.4955194238062763E-4 24-25 7.477597119031381E-5 0.0 0.0 7.290657191055597E-4 1.8693992797578455E-4 26-27 9.346996398789227E-5 0.0 0.0 0.0011216395678547073 1.8693992797578455E-4 28-29 1.1216395678547073E-4 0.0 0.0 0.003159284782790759 1.8693992797578455E-4 30-31 1.1216395678547073E-4 0.0 0.0 0.008692706650873981 2.05633920773363E-4 32-33 1.1216395678547073E-4 0.0 0.0 0.019404364523886435 2.2432791357094147E-4 34-35 1.308579495830492E-4 0.0 0.0 0.03533164638742328 2.2432791357094147E-4 36-37 1.4955194238062763E-4 0.0 0.0 0.06294267374944666 2.2432791357094147E-4 38-39 1.4955194238062763E-4 0.0 0.0 0.10001286146704473 2.2432791357094147E-4 40-41 1.4955194238062763E-4 0.0 0.0 0.13962543220511348 2.2432791357094147E-4 42-43 1.4955194238062763E-4 0.0 0.0 0.18486489477525336 2.2432791357094147E-4 44-45 1.4955194238062763E-4 0.0 0.0 0.24130205903114268 2.2432791357094147E-4 46-47 1.4955194238062763E-4 0.0 0.0 0.3115540839644425 2.2432791357094147E-4 48-49 1.4955194238062763E-4 0.0 0.0 0.386124421233983 2.2432791357094147E-4 50-51 1.4955194238062763E-4 0.0 0.0 0.46579821853726233 2.2432791357094147E-4 52-53 1.4955194238062763E-4 0.0 0.0 0.5479957048682148 2.2432791357094147E-4 54-55 1.4955194238062763E-4 0.0 0.0 0.6426994723807473 2.2432791357094147E-4 56-57 1.4955194238062763E-4 0.0 0.0 0.7548073471878253 2.2432791357094147E-4 58-59 1.4955194238062763E-4 0.0 0.0 0.8819825801897515 2.2432791357094147E-4 60-61 1.4955194238062763E-4 0.0 0.0 1.0272162102341384 2.2432791357094147E-4 62-63 1.4955194238062763E-4 0.0 0.0 1.176020392902863 2.617158991660984E-4 64-65 1.4955194238062763E-4 0.0 0.0 1.3350314956390652 2.617158991660984E-4 66-67 1.4955194238062763E-4 0.0 0.0 1.5254672002679972 2.9910388476125525E-4 68-69 1.4955194238062763E-4 0.0 0.0 1.735400739384803 3.364918703564122E-4 70-71 1.4955194238062763E-4 0.0 0.0 1.9662154684565043 3.738798559515691E-4 72-73 1.682459351782061E-4 0.0 0.0 2.2100972984937126 4.11267841546726E-4 74-75 1.8693992797578455E-4 0.0 0.0 2.4636065348216745 4.11267841546726E-4 76-77 1.8693992797578455E-4 0.0 0.0 2.739399010564349 4.2996183434430445E-4 78-79 1.8693992797578455E-4 0.0 0.0 3.0624872880848977 4.4865582714188293E-4 80-81 2.2432791357094147E-4 0.0 0.0 3.4143830085065145 4.8604381273703985E-4 82-83 2.2432791357094147E-4 1.8693992797578453E-5 0.0 3.7800562016199466 5.608197839273536E-4 84-85 2.4302190636851993E-4 3.7387985595156907E-5 0.0 4.154552959333836 5.608197839273536E-4 86-87 2.617158991660984E-4 3.7387985595156907E-5 0.0 4.554230525346063 5.608197839273536E-4 88-89 2.617158991660984E-4 3.7387985595156907E-5 0.0 4.994399279757846 5.79513776724932E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9325 0.0 21.987549 1 GAGTACT 7980 0.0 21.946703 12-13 TACATGG 9645 0.0 20.376408 2 ACATGGG 9450 0.0 19.89484 3 GTACTTT 8900 0.0 19.73161 14-15 GTATCAA 21035 0.0 19.652489 1 AGTACTT 8565 0.0 18.845057 12-13 GGTATCA 16715 0.0 18.314507 1 GTATAGG 810 0.0 18.164297 1 TCAACGC 23490 0.0 17.539532 4 ATCAACG 23585 0.0 17.428722 3 CAACGCA 23590 0.0 17.42503 5 TATCAAC 23780 0.0 17.385378 2 AGAGTAC 15335 0.0 17.362053 10-11 AACGCAG 23950 0.0 17.174204 6 ACTTTTT 10470 0.0 17.114603 16-17 CATGGGG 4605 0.0 16.042673 4 TACTTTT 10265 0.0 15.862735 14-15 ACGCAGA 25520 0.0 15.728146 7 CGCAGAG 25390 0.0 15.454473 8 >>END_MODULE