FastQCFastQC Report
Thu 26 May 2016
SRR938897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2674656
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.3389968653912877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.30329133914791284No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79240.2962623978560233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48500.18133173013651102No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39140.14633657561944413No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28470.10644359498941172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT84300.020.4064612-13
GTACATG88750.020.0824681
TTTGCGT5000.019.9300383
AGTACTT90800.019.46828512-13
GTATCAA217950.019.0447441
TACATGG92400.018.8988192
GTACTTT90900.018.66394614-15
CTTTGCG5400.018.4537372
AGAGTAC154900.018.136510-11
ACATGGG92400.017.974423
TACTTTT103100.016.93871514-15
GGTATCA174550.016.899921
ATCAACG244500.016.7878883
CAACGCA244400.016.7559245
TCAACGC245050.016.7308444
TATCAAC247750.016.6442762
AACGCAG246450.016.6165476
ACTTTTT106800.016.04024516-17
TACACCG3001.483173E-615.817495
GTGTAGC13750.015.59637451
Produced by FastQC (version 0.11.5)