##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938897_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2674656 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20662806731034 33.0 31.0 34.0 31.0 34.0 2 32.455879185958864 34.0 31.0 34.0 31.0 34.0 3 32.54656038010122 34.0 31.0 34.0 31.0 34.0 4 36.083466434562055 37.0 35.0 37.0 35.0 37.0 5 36.009177254944184 37.0 35.0 37.0 35.0 37.0 6 36.04642092291495 37.0 35.0 37.0 35.0 37.0 7 35.98710525764809 37.0 35.0 37.0 35.0 37.0 8 35.95724160415396 37.0 35.0 37.0 35.0 37.0 9 37.644292948326814 39.0 37.0 39.0 35.0 39.0 10-11 37.55384206417573 39.0 37.0 39.0 35.0 39.0 12-13 37.46733860354378 39.0 37.0 39.0 34.0 39.0 14-15 38.796396994604166 40.0 38.0 41.0 34.5 41.0 16-17 38.88667589402151 40.0 38.0 41.0 35.0 41.0 18-19 38.92629351213763 40.0 38.0 41.0 35.0 41.0 20-21 38.902150220439566 40.0 38.0 41.0 35.0 41.0 22-23 38.83072215641937 40.0 38.0 41.0 34.0 41.0 24-25 38.75796532339112 40.0 38.0 41.0 34.0 41.0 26-27 38.60080118714332 40.0 38.0 41.0 34.0 41.0 28-29 38.45058822517737 40.0 38.0 41.0 34.0 41.0 30-31 38.26831936518191 40.0 38.0 41.0 34.0 41.0 32-33 38.16623034887478 40.0 38.0 41.0 33.0 41.0 34-35 38.068106515379924 40.0 38.0 41.0 33.0 41.0 36-37 37.94178148517043 40.0 37.5 41.0 33.0 41.0 38-39 37.808906079884665 40.0 37.0 41.0 33.0 41.0 40-41 37.65521323115945 40.0 37.0 41.0 33.0 41.0 42-43 37.47977889493079 40.0 37.0 41.0 32.0 41.0 44-45 37.233177836701245 40.0 37.0 41.0 31.5 41.0 46-47 36.933454433018674 40.0 36.0 41.0 31.0 41.0 48-49 36.612384358960554 39.5 36.0 41.0 30.0 41.0 50-51 36.35085222174365 39.0 36.0 41.0 30.0 41.0 52-53 36.09695377648565 39.0 35.0 40.5 29.5 41.0 54-55 35.79966694782432 39.0 35.0 40.0 28.5 41.0 56-57 35.660978645478146 38.5 35.0 40.0 28.0 41.0 58-59 35.38094768074848 38.0 34.5 40.0 28.0 41.0 60-61 35.00940513471639 38.0 34.0 40.0 27.0 41.0 62-63 34.59477686102437 37.5 34.0 40.0 26.0 41.0 64-65 34.18473796256416 37.0 33.5 39.5 26.0 41.0 66-67 33.71707090556693 36.0 33.0 39.0 25.5 40.5 68-69 33.2287305358147 36.0 32.5 39.0 24.0 40.0 70-71 32.68783836126964 35.0 32.0 38.0 22.5 40.0 72-73 32.160632245791604 35.0 31.0 37.5 21.5 39.5 74-75 31.622877857937617 35.0 31.0 37.0 20.0 39.0 76-77 29.713649157125253 33.0 28.5 35.0 18.0 37.5 78-79 30.667695584030245 34.0 30.0 36.0 18.0 38.0 80-81 30.630540525585346 34.0 30.0 35.5 18.0 37.0 82-83 30.198992879832023 34.0 30.0 35.0 14.0 37.0 84-85 29.719770878946676 34.0 29.5 35.0 7.5 36.5 86-87 29.0067029180575 34.0 29.0 35.0 2.0 36.0 88-89 28.290167969264083 33.0 27.5 35.0 2.0 35.5 90-91 27.68700012263259 33.0 26.5 35.0 2.0 35.0 92-93 27.25351783556465 33.0 26.0 34.5 2.0 35.0 94-95 26.672272247346946 32.0 25.0 34.0 2.0 35.0 96-97 25.790124225320938 32.0 24.0 34.0 2.0 35.0 98-99 24.532021127202903 32.0 10.5 34.0 2.0 35.0 100-101 18.83824667546032 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0807050525620383 1101 2 0.09286832011009238 1101 3 0.11055810766414709 1101 4 0.04802659067453163 1101 5 0.06499465922773595 1101 6 0.07846256646222827 1101 7 0.05676381440533618 1101 8 0.11560270980116627 1101 9 0.15757430263955996 1101 10-11 0.16051196545895863 1101 12-13 0.164679007133266 1101 14-15 0.13161530138647493 1101 16-17 0.16637358444310024 1101 18-19 0.19103009523061587 1101 20-21 0.19233375429086408 1101 22-23 0.2178397895006441 1101 24-25 0.14980400911077396 1101 26-27 0.13637704633546832 1101 28-29 0.07312896487385956 1101 30-31 0.15069772491122535 1101 32-33 0.20703961156652184 1101 34-35 0.27528298145002594 1101 36-37 0.3026853496656017 1101 38-39 0.28509365056468283 1101 40-41 0.28703856946315653 1101 42-43 0.3078364043912458 1101 44-45 0.33493717845432514 1101 46-47 0.27747199489196106 1101 48-49 0.48670557051636365 1101 50-51 0.3089723180597943 1101 52-53 0.28251288655640394 1101 54-55 0.3191905457686346 1101 56-57 0.14319421315306613 1101 58-59 0.22454678629494396 1101 60-61 0.18241487515191324 1101 62-63 0.295606094703011 1101 64-65 0.2546262256706555 1101 66-67 0.03575302238471778 1101 68-69 0.23491690826134004 1101 70-71 0.26213108518341244 1101 72-73 0.2854778353183107 1101 74-75 0.15438889329428207 1101 76-77 -0.15780190128434768 1101 78-79 0.25662669044814024 1101 80-81 0.3816921633778527 1101 82-83 0.4325487588840602 1101 84-85 0.4045277705750969 1101 86-87 0.6269447742763852 1101 88-89 0.6117319829111274 1101 90-91 0.5675856593631927 1101 92-93 0.43648202115408097 1101 94-95 0.32379542909064085 1101 96-97 0.38017161121803156 1101 98-99 0.6170457751646978 1101 100-101 1.1365591941898323 1102 1 0.2002130767661896 1102 2 0.18048780621024463 1102 3 0.1932644130223835 1102 4 0.13728599609415681 1102 5 0.14441893354151603 1102 6 0.13634426426105506 1102 7 0.1614380229469461 1102 8 0.18274951327394007 1102 9 0.24943609339637618 1102 10-11 0.3625783076496134 1102 12-13 0.2805871014944046 1102 14-15 0.3626794950197265 1102 16-17 0.3540972098048414 1102 18-19 0.3258707073502265 1102 20-21 0.34674597037435717 1102 22-23 0.36168241863332184 1102 24-25 0.36387706006227205 1102 26-27 0.4288757308921731 1102 28-29 0.37300399775993753 1102 30-31 0.3894616466862004 1102 32-33 0.4096770753918264 1102 34-35 0.5122557079255117 1102 36-37 0.5175082033006149 1102 38-39 0.5421564849065277 1102 40-41 0.4500088404444753 1102 42-43 0.564361003181034 1102 44-45 0.5624990042896343 1102 46-47 0.6528995139658846 1102 48-49 0.6736399298954723 1102 50-51 0.5518174026866447 1102 52-53 0.5454119043587369 1102 54-55 0.5490993416995309 1102 56-57 0.5748854347614198 1102 58-59 0.49369525599590247 1102 60-61 0.5385185320843036 1102 62-63 0.6331937909233005 1102 64-65 0.4722334342999659 1102 66-67 0.4171647122908837 1102 68-69 0.43371547004333166 1102 70-71 0.6595232188324545 1102 72-73 0.757864487953583 1102 74-75 0.6734880338713225 1102 76-77 0.5360286696180481 1102 78-79 0.7102704684013119 1102 80-81 0.7757244355869197 1102 82-83 0.7433047003029856 1102 84-85 0.932916133286664 1102 86-87 0.9847705657347738 1102 88-89 0.8125398843405769 1102 90-91 0.8074194750257355 1102 92-93 0.9462163016065972 1102 94-95 0.8862603506025231 1102 96-97 1.1655690943365116 1102 98-99 1.5857765916165079 1102 100-101 1.790044022743725 1103 1 0.048578055669146636 1103 2 0.05700729586263975 1103 3 0.05677531316302975 1103 4 0.0693196006752288 1103 5 0.07151859197028898 1103 6 0.07680036231209186 1103 7 0.11979144870543479 1103 8 0.09523821128478716 1103 9 0.10176932626758628 1103 10-11 0.10053352665705262 1103 12-13 0.12844286299731777 1103 14-15 0.11112170418187617 1103 16-17 0.08091306272829257 1103 18-19 0.17729224642999242 1103 20-21 0.16195154099923315 1103 22-23 0.19249226894476124 1103 24-25 0.14310149245126524 1103 26-27 0.1433159056962694 1103 28-29 0.21011219546940652 1103 30-31 0.16776685062431795 1103 32-33 0.19217406152804983 1103 34-35 0.15452200790542037 1103 36-37 0.08419307872757287 1103 38-39 0.0848447258147047 1103 40-41 0.18156587781638933 1103 42-43 0.20257679582720556 1103 44-45 0.2689260687863211 1103 46-47 0.2687825760514784 1103 48-49 0.23488164177010162 1103 50-51 0.31244252826429886 1103 52-53 0.3292192933480891 1103 54-55 0.3862458405944409 1103 56-57 0.49330642129949354 1103 58-59 0.21820424735616228 1103 60-61 0.23243429459183318 1103 62-63 0.41355156166873286 1103 64-65 0.32877351868814486 1103 66-67 0.372494228063438 1103 68-69 0.48886792131919776 1103 70-71 0.5398087824249771 1103 72-73 0.3293715454179207 1103 74-75 0.31300606682290066 1103 76-77 0.06930360934077484 1103 78-79 0.28090036592994494 1103 80-81 0.41290735200243134 1103 82-83 0.5492878477813896 1103 84-85 0.5184866325834321 1103 86-87 0.4296212539003328 1103 88-89 0.3292165972198191 1103 90-91 0.42532998838720104 1103 92-93 0.5148099574233527 1103 94-95 0.7319800361560702 1103 96-97 0.4305561598378169 1103 98-99 0.7139097541642272 1103 100-101 1.2369686259183261 1104 1 0.010801388623541186 1104 2 0.04755594675553709 1104 3 0.006236776248556453 1104 4 0.007132689164194517 1104 5 0.057881711598199104 1104 6 0.0302037747449333 1104 7 0.08077694685439951 1104 8 0.017073438080096537 1104 9 0.028654900533965133 1104 10-11 0.1463826096645775 1104 12-13 0.09867837288516768 1104 14-15 0.14152750825859783 1104 16-17 0.1122731920290434 1104 18-19 0.08854347592401268 1104 20-21 0.12883436280538518 1104 22-23 0.061691173274056155 1104 24-25 0.06370398158970403 1104 26-27 0.025507673399744135 1104 28-29 0.01423742592230326 1104 30-31 0.09879285411385297 1104 32-33 0.06846503024576833 1104 34-35 0.16693734890156975 1104 36-37 0.2603456675847795 1104 38-39 0.2644110140772966 1104 40-41 0.23280480928104907 1104 42-43 0.20472563009388267 1104 44-45 0.2666796164017171 1104 46-47 0.2726304598389646 1104 48-49 0.1179039357711531 1104 50-51 0.14410425430090612 1104 52-53 0.17490566919514094 1104 54-55 0.20592579218079976 1104 56-57 0.10731897696653192 1104 58-59 0.2650203212295992 1104 60-61 -0.008924802922486208 1104 62-63 -0.03102320330638264 1104 64-65 0.013216774141170617 1104 66-67 -0.01872489866217819 1104 68-69 0.009049385986386937 1104 70-71 0.02614456280151245 1104 72-73 0.0391541937356763 1104 74-75 -0.002943775999568743 1104 76-77 -0.14249627413532195 1104 78-79 0.20473784535600004 1104 80-81 0.27026928723837074 1104 82-83 0.12328523865915741 1104 84-85 0.17321343468802297 1104 86-87 0.1621858394063942 1104 88-89 0.18939501595458808 1104 90-91 0.2973984328980954 1104 92-93 0.376033523301988 1104 94-95 0.6288488886859867 1104 96-97 0.5204782784969666 1104 98-99 0.9182271007802179 1104 100-101 1.032300039428673 1105 1 -0.023042737187296325 1105 2 0.04184808675656626 1105 3 0.04739877994745001 1105 4 0.00835465611630326 1105 5 0.03136060943668184 1105 6 0.02066504683764947 1105 7 0.014298857203129955 1105 8 0.0590230459428156 1105 9 0.04565240938013204 1105 10-11 0.16417945198331552 1105 12-13 0.061244170523565344 1105 14-15 0.1519098383198383 1105 16-17 0.02897262508723486 1105 18-19 0.09455380074262365 1105 20-21 0.14918690798971568 1105 22-23 0.04255635735610497 1105 24-25 0.054611648471286856 1105 26-27 -0.012583294578490722 1105 28-29 0.07477586929925195 1105 30-31 0.08681306787482868 1105 32-33 0.15876345412936388 1105 34-35 0.20648789692514669 1105 36-37 0.21832031108946381 1105 38-39 0.3118731700301822 1105 40-41 0.41476520810781636 1105 42-43 0.32059023906143125 1105 44-45 0.30788936973814174 1105 46-47 0.21245100898249092 1105 48-49 0.28154413809038203 1105 50-51 0.2486012712728396 1105 52-53 0.24191891801690701 1105 54-55 0.34601702350093433 1105 56-57 0.2976613668396695 1105 58-59 0.2220636576201187 1105 60-61 0.32520181004665716 1105 62-63 0.39504779609126217 1105 64-65 0.21099312422244765 1105 66-67 0.33821030106903294 1105 68-69 0.2865024763187094 1105 70-71 0.34129232182248614 1105 72-73 0.35147836761397144 1105 74-75 0.37736861711237424 1105 76-77 0.15488249021605682 1105 78-79 0.28493824248769073 1105 80-81 0.3089562554466454 1105 82-83 0.428262249214022 1105 84-85 0.3357908102892466 1105 86-87 0.5866577934940942 1105 88-89 0.7353850301508373 1105 90-91 0.6804168910464092 1105 92-93 0.8426448730351659 1105 94-95 0.9038360242828496 1105 96-97 0.902838381896494 1105 98-99 0.9294412280137045 1105 100-101 0.6099821918097987 1106 1 0.05800396624722026 1106 2 0.036598192250203 1106 3 0.030400140298958434 1106 4 -0.008252550500728262 1106 5 0.06018545458972824 1106 6 0.09811685173787055 1106 7 0.10085409898274378 1106 8 0.11216247754605746 1106 9 0.09121046333976324 1106 10-11 0.1822840042919367 1106 12-13 0.1257094874618616 1106 14-15 0.07633213155968832 1106 16-17 -0.05595794091932049 1106 18-19 -0.044769726846539015 1106 20-21 0.05729250785751816 1106 22-23 0.08021788065470048 1106 24-25 0.15413648447663064 1106 26-27 0.12690149810807583 1106 28-29 0.10299158091653737 1106 30-31 0.12668302299208278 1106 32-33 0.22854387982201274 1106 34-35 0.1603792842181022 1106 36-37 0.15913801218393786 1106 38-39 0.2542805053051893 1106 40-41 0.3144812605506999 1106 42-43 0.2625347302280048 1106 44-45 0.2994706086908181 1106 46-47 0.36111728908506535 1106 48-49 0.5195258308870052 1106 50-51 0.3971996813435723 1106 52-53 0.40874286610519306 1106 54-55 0.38209702751412067 1106 56-57 0.5374474434501835 1106 58-59 0.4514314630278804 1106 60-61 0.5571918331519683 1106 62-63 0.6253262402740631 1106 64-65 0.6259099981877938 1106 66-67 0.6362031924209148 1106 68-69 0.5140122658330881 1106 70-71 0.633839159521628 1106 72-73 0.7685645743578959 1106 74-75 0.5766365798015585 1106 76-77 0.38191932888898705 1106 78-79 0.6780619016857052 1106 80-81 0.7622075988306491 1106 82-83 0.7303011534966224 1106 84-85 0.6803693964089419 1106 86-87 0.8046854756144732 1106 88-89 0.8401609410109288 1106 90-91 1.0456580657291141 1106 92-93 1.156523187969821 1106 94-95 1.1901617620743572 1106 96-97 1.0776332963655015 1106 98-99 1.1614811946098484 1106 100-101 1.1026067111866915 1107 1 -0.010381483638617794 1107 2 0.04114938266878454 1107 3 0.07717754816891897 1107 4 -0.002428922131201716 1107 5 -0.023161109228283294 1107 6 0.010366884061660642 1107 7 0.014403295077308087 1107 8 -0.014465179054610644 1107 9 -0.013167442641695004 1107 10-11 0.016816508306220612 1107 12-13 0.05023259410004499 1107 14-15 0.11081132839823482 1107 16-17 0.01792096511341157 1107 18-19 -0.04368480302596822 1107 20-21 -0.04018175770612231 1107 22-23 0.0925666540479213 1107 24-25 0.048275873351038 1107 26-27 -0.006297531961365621 1107 28-29 0.0630939046904544 1107 30-31 0.02408635678528981 1107 32-33 0.19347801512255813 1107 34-35 0.13607727956491544 1107 36-37 0.04014231070701868 1107 38-39 0.019448961036758305 1107 40-41 0.05867876002520944 1107 42-43 0.09396703914380566 1107 44-45 0.01368383305985077 1107 46-47 -0.03820466964304359 1107 48-49 0.020114810170163366 1107 50-51 0.011847597560290524 1107 52-53 -0.04322736855473153 1107 54-55 -0.032649702003972436 1107 56-57 0.11451300622010052 1107 58-59 0.006800282952923453 1107 60-61 0.04108923778593265 1107 62-63 0.19204631042573084 1107 64-65 0.20697410476169864 1107 66-67 0.21848809110477418 1107 68-69 0.2074783085120515 1107 70-71 0.2173561907308894 1107 72-73 0.22100641138962374 1107 74-75 0.17909441620920674 1107 76-77 0.17332423300469202 1107 78-79 0.3648968350770154 1107 80-81 0.2113182430023599 1107 82-83 0.20302579304115653 1107 84-85 0.351846662487592 1107 86-87 0.4688309103027066 1107 88-89 0.7629077196467406 1107 90-91 0.7944898945914112 1107 92-93 0.7689415207265036 1107 94-95 1.0550756724301813 1107 96-97 1.1454096864873478 1107 98-99 1.2426254064112427 1107 100-101 1.4423884551403354 1108 1 0.02897929334019267 1108 2 0.023732318735930846 1108 3 0.05804245556055321 1108 4 -0.02258105636622787 1108 5 0.015476525230560867 1108 6 0.11941882583045071 1108 7 0.10080285015927615 1108 8 0.014483882955090621 1108 9 0.13379884034848288 1108 10-11 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5195.0 13 7189.0 14 8985.0 15 11779.0 16 16010.0 17 22384.0 18 22312.0 19 16320.0 20 15706.0 21 16885.0 22 18451.0 23 20524.0 24 23355.0 25 27976.0 26 33028.0 27 40017.0 28 48112.0 29 58277.0 30 70087.0 31 85174.0 32 105536.0 33 133199.0 34 178996.0 35 254664.0 36 398599.0 37 600649.0 38 404429.0 39 22334.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.85072746513585 20.01536128872653 15.369021926625445 22.76488931951218 2 15.190177727528326 21.13767153607791 40.11734593158896 23.554804804804803 3 19.111653984661952 25.015665565964373 30.575483351877775 25.297197097495904 4 13.061343215725685 17.76015308136822 37.450797410956774 31.72770629194932 5 13.514186497254228 37.62880161037532 34.61469437565055 14.242317516719908 6 31.039281313185697 36.88709875213859 17.60151585848797 14.472104076187742 7 27.59180993742747 33.17447178253951 21.347006867425193 17.886711412607827 8 25.34494155510092 35.70156311690177 19.96911004630128 18.98438528169604 9 25.709287474725723 17.541657693550125 21.047080446980846 35.70197438474331 10-11 24.473240670949835 26.959747346948543 27.63766630175993 20.929345680341697 12-13 25.77529596329397 24.37197531196535 27.972475712764556 21.88025301197612 14-15 22.49894751954847 25.722996118347407 26.703429329022217 25.07462703308191 16-17 21.66702840415069 28.965432665137836 27.333912487196642 22.03362644351483 18-19 21.576467249686264 27.88911473457067 29.28980869751183 21.24460931823123 20-21 22.447727857339412 26.7825096012347 29.184463347809963 21.585299193615924 22-23 22.096019076845767 26.795277598315447 29.135802884557865 21.972900440280917 24-25 22.230065401019385 26.952398489149527 29.121054043469197 21.69648206636189 26-27 22.053321997296102 26.963691779428835 29.344540008135628 21.638446215139442 28-29 21.65489692880131 27.38843798978261 29.315209133436227 21.64145594797985 30-31 21.93882129141093 26.933108407211993 29.16633017479631 21.961740126580764 32-33 21.782838615507938 27.098550243470566 29.191417513130663 21.92719362789084 34-35 22.367710583153347 26.963980693178897 28.95669800709572 21.71161071657204 36-37 22.25060667561955 26.784471497558528 29.17211262981026 21.792809197011668 38-39 21.9716501350412 27.125613555945343 29.246468980159435 21.65626732885402 40-41 22.112587715239435 27.00734812305512 29.145454698414746 21.7346094632907 42-43 21.879448229756456 27.13089718188579 28.769135683836982 22.220518904520777 44-45 22.133478351171114 27.032242228643504 28.629968404780726 22.204311015404656 46-47 22.347820072309066 27.31082430093134 28.28675368181767 22.05460194494192 48-49 22.366467469760593 27.383959981649408 28.15681232106957 22.09276022752043 50-51 21.996416343492637 27.535549143483664 28.32333667022789 22.144697842795814 52-53 22.39022513549406 27.6702873191917 27.851226475479464 22.088261069834775 54-55 22.01387767249321 27.30300644269768 28.115783861550792 22.56733202325832 56-57 22.25235693861192 27.615027876482063 27.91095378246773 22.221661402438293 58-59 22.51010371697516 27.34425956144662 27.938826781752795 22.206809939825423 60-61 22.292620697700155 27.295806201712697 28.40690028026453 22.004672820322618 62-63 22.178891000301913 27.294893568893496 28.420437688753626 22.105777742050968 64-65 21.99672889273075 27.553087259375552 28.098882480732378 22.351301367161323 66-67 22.022859999375605 27.50406651949116 27.86630970156299 22.606763779570247 68-69 21.840728366387648 27.477503077109443 28.12558420212758 22.55618435437533 70-71 22.135351237691875 27.356003912278815 27.92544162688585 22.58320322314346 72-73 22.110170429393534 27.11556177691636 28.172707069619417 22.601560724070684 74-75 21.899507824557627 27.70163714511324 27.68159718483424 22.7172578454949 76-77 22.01922103554681 27.304042158215307 27.86138894351667 22.815347862721218 78-79 22.02212700406796 27.315184553720982 27.93177195501316 22.730916487197895 80-81 22.03009739592096 27.45383508122558 27.79107546781822 22.724992055035237 82-83 22.00755910292763 27.337160367538853 27.98782348085137 22.66745704868215 84-85 21.88125373296157 27.346187962735346 27.794806988945293 22.97775131535779 86-87 21.90197609169317 27.333084584748295 27.92864034322814 22.8362989803304 88-89 21.932480200221132 27.215081482549753 27.721286564993754 23.13115175223536 90-91 21.82367866733008 27.127252552594207 27.887404887114535 23.161663892961183 92-93 21.930222054724048 27.28745304069009 27.811015696971875 22.971309207613988 94-95 21.795516266121197 27.144605586543303 27.729884224826414 23.329993922509093 96-97 21.621677703600014 27.070546642259792 27.887511515499565 23.420264138640633 98-99 21.56722210258067 27.1723728210282 27.5954178780374 23.66498719835373 100-101 21.65576304303448 26.30662151178137 28.580455133127895 23.45716031205625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 764.0 1 542.0 2 346.5 3 378.5 4 351.5 5 384.0 6 645.0 7 1407.0 8 2337.0 9 2810.0 10 2651.5 11 2374.5 12 2527.5 13 2762.0 14 2967.0 15 3144.5 16 3281.0 17 3486.0 18 3739.0 19 4079.5 20 4669.0 21 5510.5 22 6592.0 23 8004.5 24 9921.0 25 12668.5 26 16115.0 27 19801.5 28 23127.0 29 27195.0 30 32108.5 31 37174.5 32 42092.0 33 46951.0 34 53378.0 35 59220.5 36 65301.0 37 72168.0 38 76229.5 39 78610.0 40 80864.0 41 83647.0 42 86621.5 43 90494.0 44 95339.5 45 98336.5 46 99127.5 47 105951.0 48 122941.5 49 122601.5 50 106934.0 51 98307.5 52 92002.0 53 87891.5 54 83130.5 55 76263.0 56 69770.5 57 62857.0 58 55847.0 59 48590.5 60 40741.0 61 32286.5 62 25143.0 63 19845.5 64 14996.5 65 11254.0 66 8027.5 67 5864.0 68 4150.0 69 2556.5 70 1662.5 71 1111.5 72 760.5 73 494.5 74 335.0 75 217.5 76 127.0 77 58.5 78 30.0 79 14.0 80 7.0 81 7.5 82 5.0 83 3.5 84 2.0 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4044258401828123 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.225356830934521E-4 16-17 0.0024115250708876207 18-19 6.54289747915246E-4 20-21 0.0 22-23 0.0 24-25 1.3085794958304917E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013758778699017742 36-37 0.024563906536018087 38-39 0.010730351865810032 40-41 0.006112935644808155 42-43 0.005122154026536496 44-45 0.0015889893877941686 46-47 9.720876254740796E-4 48-49 0.0031032028043980232 50-51 5.047378055346183E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.038416902958736E-4 60-61 0.0014955194238062764 62-63 0.001439437445413541 64-65 9.90781618271658E-4 66-67 0.002673240970053719 68-69 0.00254238302047067 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09464768553413971 78-79 0.0035892466171350635 80-81 2.2432791357094147E-4 82-83 0.0 84-85 1.3085794958304917E-4 86-87 0.09773219434574015 88-89 0.4486558271418829 90-91 0.3982007405812187 92-93 0.0 94-95 0.0012151095318425997 96-97 0.0 98-99 0.0 100-101 3.738798559515691E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2674656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37736341390763 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7073091305112 39.34301825720529 2 14.487549716214149 15.466144123588338 3 5.037648723755652 8.066892200385402 4 2.3248634641634163 4.963803280574675 5 1.2354962473820592 3.2973766096515646 6 0.7616694984581472 2.439354577229357 7 0.4928018081490145 1.841312284322051 8 0.350765836314647 1.4978364414520118 9 0.2418673036225908 1.161921506706447 >10 1.2313856583544638 11.750438099371395 >50 0.08340536278224998 3.0719574608656828 >100 0.04110354783230944 3.851323445212771 >500 0.0025923218817843845 0.8804154901024063 >1k 0.0013311923176429364 1.4294130006825814 >5k 2.1018826068046364E-4 0.9387932226501712 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9067 0.3389968653912877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8112 0.30329133914791284 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7924 0.2962623978560233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4850 0.18133173013651102 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3914 0.14633657561944413 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2847 0.10644359498941172 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.7387985595156907E-5 0.0 0.0 0.0 0.0 7 3.7387985595156907E-5 0.0 0.0 0.0 0.0 8 3.7387985595156907E-5 0.0 0.0 0.0 0.0 9 3.7387985595156907E-5 0.0 0.0 0.0 0.0 10-11 3.7387985595156907E-5 0.0 0.0 1.8693992797578453E-5 1.8693992797578453E-5 12-13 3.7387985595156907E-5 0.0 0.0 5.608197839273536E-5 3.7387985595156907E-5 14-15 3.7387985595156907E-5 0.0 0.0 7.477597119031381E-5 3.7387985595156907E-5 16-17 3.7387985595156907E-5 0.0 0.0 7.477597119031381E-5 3.7387985595156907E-5 18-19 3.7387985595156907E-5 0.0 0.0 1.4955194238062763E-4 3.7387985595156907E-5 20-21 3.7387985595156907E-5 0.0 0.0 2.617158991660984E-4 3.7387985595156907E-5 22-23 3.7387985595156907E-5 0.0 0.0 4.11267841546726E-4 9.346996398789227E-5 24-25 3.7387985595156907E-5 0.0 0.0 5.234317983321968E-4 1.4955194238062763E-4 26-27 3.7387985595156907E-5 0.0 0.0 9.533936326765011E-4 1.4955194238062763E-4 28-29 3.7387985595156907E-5 0.0 0.0 0.003084508811600445 1.4955194238062763E-4 30-31 3.7387985595156907E-5 0.0 0.0 0.008636624672481247 1.4955194238062763E-4 32-33 3.7387985595156907E-5 0.0 0.0 0.019516528480671907 1.4955194238062763E-4 34-35 3.7387985595156907E-5 0.0 0.0 0.0358363841929579 1.4955194238062763E-4 36-37 7.477597119031381E-5 0.0 0.0 0.06380259741813527 1.4955194238062763E-4 38-39 7.477597119031381E-5 0.0 0.0 0.10137752294126795 1.4955194238062763E-4 40-41 7.477597119031381E-5 0.0 0.0 0.14138266752808587 1.4955194238062763E-4 42-43 7.477597119031381E-5 0.0 0.0 0.18677168204060635 1.4955194238062763E-4 44-45 7.477597119031381E-5 0.0 0.0 0.24352664417405454 2.05633920773363E-4 46-47 7.477597119031381E-5 0.0 0.0 0.3141338549705084 3.738798559515691E-4 48-49 7.477597119031381E-5 0.0 0.0 0.3892463180311786 4.4865582714188293E-4 50-51 7.477597119031381E-5 0.0 0.0 0.46972395702475384 4.4865582714188293E-4 52-53 7.477597119031381E-5 0.0 0.0 0.5524448751540385 4.8604381273703985E-4 54-55 7.477597119031381E-5 0.0 0.0 0.6480085663352595 5.234317983321968E-4 56-57 7.477597119031381E-5 0.0 0.0 0.7610885287678116 5.234317983321968E-4 58-59 7.477597119031381E-5 0.0 0.0 0.8891984614096168 5.234317983321968E-4 60-61 7.477597119031381E-5 0.0 0.0 1.0357406709498342 5.608197839273536E-4 62-63 7.477597119031381E-5 0.0 0.0 1.186582498833495 5.608197839273536E-4 64-65 7.477597119031381E-5 0.0 0.0 1.3473134489070744 5.608197839273536E-4 66-67 7.477597119031381E-5 0.0 0.0 1.5396933287869543 5.608197839273536E-4 68-69 7.477597119031381E-5 0.0 0.0 1.75016899369489 5.608197839273536E-4 70-71 7.477597119031381E-5 0.0 0.0 1.982853122046349 5.982077695225105E-4 72-73 7.477597119031381E-5 0.0 0.0 2.2284174114353394 7.103717263079813E-4 74-75 7.477597119031381E-5 0.0 0.0 2.484356866826986 7.103717263079813E-4 76-77 7.477597119031381E-5 0.0 0.0 2.7623552337197754 7.103717263079813E-4 78-79 7.477597119031381E-5 0.0 0.0 3.0874624624624625 7.103717263079813E-4 80-81 7.477597119031381E-5 0.0 0.0 3.4406480683871123 7.103717263079813E-4 82-83 7.477597119031381E-5 0.0 0.0 3.80828413074429 7.851476974982952E-4 84-85 7.477597119031381E-5 0.0 0.0 4.185061555579484 7.851476974982952E-4 86-87 7.477597119031381E-5 0.0 0.0 4.586477662921887 7.851476974982952E-4 88-89 7.477597119031381E-5 0.0 0.0 5.029001860426163 7.851476974982952E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8430 0.0 20.40646 12-13 GTACATG 8875 0.0 20.082468 1 TTTGCGT 500 0.0 19.930038 3 AGTACTT 9080 0.0 19.468285 12-13 GTATCAA 21795 0.0 19.044744 1 TACATGG 9240 0.0 18.898819 2 GTACTTT 9090 0.0 18.663946 14-15 CTTTGCG 540 0.0 18.453737 2 AGAGTAC 15490 0.0 18.1365 10-11 ACATGGG 9240 0.0 17.97442 3 TACTTTT 10310 0.0 16.938715 14-15 GGTATCA 17455 0.0 16.89992 1 ATCAACG 24450 0.0 16.787888 3 CAACGCA 24440 0.0 16.755924 5 TCAACGC 24505 0.0 16.730844 4 TATCAAC 24775 0.0 16.644276 2 AACGCAG 24645 0.0 16.616547 6 ACTTTTT 10680 0.0 16.040245 16-17 TACACCG 300 1.483173E-6 15.81749 5 GTGTAGC 1375 0.0 15.5963745 1 >>END_MODULE