FastQCFastQC Report
Thu 26 May 2016
SRR938896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684453
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92300.3438316856357701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75260.2803550667491664No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73270.2729420109050149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.16968075060356802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43330.16141090941059502No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32090.11954018192905594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90350.021.935441
GAGTACT80850.021.6860112-13
TACATGG92900.020.1739712
GTACTTT89000.019.70052114-15
ACATGGG92100.019.4756323
GTATCAA205250.019.4503381
AGTACTT86200.019.40678612-13
TTTGCGT4950.019.1221523
TACGTTG2051.641366E-518.46925
AGAGTAC152400.017.51287710-11
CATGGGG46000.017.2847614
ATCAACG229350.017.2718413
GGTATCA165100.017.2306821
TATCAAC231250.017.2117962
TCAACGC230100.017.1949774
CAACGCA229650.017.1874525
AACGCAG232500.016.9742366
ACTTTTT110750.016.30327616-17
ACGCAGA249450.015.6311477
CGCAGAG247550.015.541148
Produced by FastQC (version 0.11.5)