##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938896_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2684453 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.665930824641 33.0 31.0 34.0 30.0 34.0 2 31.74395267862764 33.0 31.0 34.0 30.0 34.0 3 31.78211836824858 34.0 31.0 34.0 30.0 34.0 4 35.31785656146708 37.0 35.0 37.0 33.0 37.0 5 35.35404456699372 37.0 35.0 37.0 33.0 37.0 6 35.43043927384834 37.0 35.0 37.0 33.0 37.0 7 35.33466855258781 37.0 35.0 37.0 33.0 37.0 8 35.31415338618333 37.0 35.0 37.0 33.0 37.0 9 36.89908707658506 39.0 37.0 39.0 33.0 39.0 10-11 36.784021363011384 39.0 37.0 39.0 32.0 39.0 12-13 36.65503698518842 39.0 37.0 39.0 32.0 39.0 14-15 37.833359347323274 40.0 38.0 41.0 32.0 41.0 16-17 37.754026798010614 40.0 38.0 41.0 32.0 41.0 18-19 37.69184820892748 40.0 37.5 41.0 32.0 41.0 20-21 37.56366529792103 40.0 37.0 41.0 32.0 41.0 22-23 37.65461660159444 40.0 37.5 41.0 32.0 41.0 24-25 37.59005354163399 40.0 37.0 41.0 32.0 41.0 26-27 37.50493508360921 40.0 37.0 41.0 32.0 41.0 28-29 37.37180386469795 40.0 37.0 41.0 31.0 41.0 30-31 37.19256734984744 40.0 37.0 41.0 31.0 41.0 32-33 37.004592183212 40.0 36.5 41.0 30.5 41.0 34-35 36.685846613816665 40.0 36.0 41.0 30.0 41.0 36-37 36.4211025486384 39.0 36.0 41.0 30.0 41.0 38-39 36.21764974093419 39.0 35.0 41.0 29.5 41.0 40-41 36.074706094686704 39.0 35.0 41.0 29.0 41.0 42-43 35.87147847252308 39.0 35.0 41.0 27.5 41.0 44-45 35.797180840938545 39.0 35.0 41.0 27.0 41.0 46-47 35.5764189203536 39.0 35.0 41.0 26.0 41.0 48-49 35.39977716130623 39.0 35.0 41.0 25.5 41.0 50-51 34.25086768142337 38.0 34.0 40.0 23.5 40.5 52-53 34.40612817583322 38.0 33.5 39.5 24.0 40.5 54-55 34.90372656924893 39.0 35.0 40.0 24.0 41.0 56-57 34.80733616867198 39.0 34.5 41.0 24.0 41.0 58-59 34.5994869345822 38.0 34.0 40.0 23.0 41.0 60-61 34.32407570555342 38.0 34.0 40.0 23.0 41.0 62-63 33.971743777968925 38.0 34.0 40.0 22.0 41.0 64-65 33.56845547305168 37.0 33.0 40.0 21.5 41.0 66-67 33.17769094858431 36.5 33.0 39.5 20.0 41.0 68-69 32.78366281696867 36.0 32.0 39.0 19.0 41.0 70-71 32.35823424734946 35.5 32.0 39.0 18.0 40.0 72-73 31.916190747239753 35.0 31.5 38.0 15.0 40.0 74-75 31.442397017194935 35.0 31.0 37.0 12.0 39.5 76-77 31.019483671347572 35.0 31.0 37.0 8.0 39.0 78-79 30.57794362575914 35.0 30.5 36.0 7.0 39.0 80-81 30.124637123466123 34.0 30.0 36.0 2.0 37.5 82-83 29.72333991319647 34.0 30.0 35.0 2.0 37.0 84-85 29.353719547334222 34.0 29.0 35.0 2.0 37.0 86-87 28.94922336133283 34.0 29.0 35.0 2.0 36.0 88-89 28.52019796956773 34.0 29.0 35.0 2.0 36.0 90-91 28.07527585694367 34.0 28.0 35.0 2.0 35.5 92-93 27.639104875369398 34.0 27.0 35.0 2.0 35.0 94-95 27.143683461770426 33.5 26.0 35.0 2.0 35.0 96-97 26.529495208148553 33.0 25.0 35.0 2.0 35.0 98-99 25.775918967476798 33.0 23.5 35.0 2.0 35.0 100-101 23.046926506070324 29.5 8.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3779641143362191 1101 2 0.351708875397037 1101 3 0.3343532301825576 1101 4 0.3187722336611074 1101 5 0.030386786650048236 1101 6 0.22099758910879075 1101 7 0.18012926604108515 1101 8 0.2883073489249455 1101 9 0.2520431924070863 1101 10-11 0.4480864795647861 1101 12-13 0.5011315722745451 1101 14-15 0.22770786404071686 1101 16-17 0.4099825913424624 1101 18-19 0.40365606777012886 1101 20-21 0.5468587424238223 1101 22-23 0.5156658076038383 1101 24-25 0.5517222697849746 1101 26-27 0.5756783895011921 1101 28-29 0.5568138306823585 1101 30-31 0.5620229018196667 1101 32-33 0.5805374557006715 1101 34-35 0.5205899924923614 1101 36-37 0.5384219043334255 1101 38-39 0.5545431793630584 1101 40-41 0.4779697393648874 1101 42-43 0.46285334850529125 1101 44-45 0.6735356108800374 1101 46-47 0.7223193588938344 1101 48-49 0.6817732269615604 1101 50-51 0.7423659594446548 1101 52-53 0.5002354951315553 1101 54-55 0.48769004738216637 1101 56-57 0.5350538322686518 1101 58-59 0.4940228155549633 1101 60-61 0.4909364618448748 1101 62-63 0.5825475146571364 1101 64-65 0.4878632861942478 1101 66-67 0.5539053275152952 1101 68-69 0.5205283480242358 1101 70-71 0.5008603538625707 1101 72-73 0.4932046740059768 1101 74-75 0.4804554663077596 1101 76-77 0.4776121007317293 1101 78-79 0.4453753151715034 1101 80-81 0.26458486809897863 1101 82-83 0.21134060967601798 1101 84-85 0.10762856397941789 1101 86-87 0.12030389059166424 1101 88-89 0.05650799647842675 1101 90-91 -0.05935927817554898 1101 92-93 -0.016519995745635896 1101 94-95 -0.006496030677151765 1101 96-97 -0.0287408330811445 1101 98-99 -0.0743301782918735 1101 100-101 0.21974550128991055 1102 1 0.1480052369489151 1102 2 0.12057020610753 1102 3 0.10381022822575403 1102 4 0.21962566912156944 1102 5 0.28197315719825866 1102 6 0.36986521366279845 1102 7 0.3798072229476972 1102 8 0.33528426886616813 1102 9 0.4710188268489901 1102 10-11 0.315706021584397 1102 12-13 0.3691511621662471 1102 14-15 0.4880453197605519 1102 16-17 0.4688402040681794 1102 18-19 0.5398419925246785 1102 20-21 0.5694509675830375 1102 22-23 0.5376010065312116 1102 24-25 0.5452203527750115 1102 26-27 0.5834481879220164 1102 28-29 0.49365410860779946 1102 30-31 0.6039383200959989 1102 32-33 0.6030320230080477 1102 34-35 0.33146941132030605 1102 36-37 0.37953212374920753 1102 38-39 0.45121555260985247 1102 40-41 0.39899354601023873 1102 42-43 0.37950487795178844 1102 44-45 0.5626827100317655 1102 46-47 0.4820221145159209 1102 48-49 0.490889792467037 1102 50-51 0.34023735240708675 1102 52-53 0.3600847830949476 1102 54-55 0.4558727243676586 1102 56-57 0.37763560211445935 1102 58-59 0.3108848789271619 1102 60-61 0.26756045268842854 1102 62-63 0.2194443824979615 1102 64-65 0.294599612424328 1102 66-67 0.303708037391587 1102 68-69 0.29548228328633996 1102 70-71 0.2419953598435498 1102 72-73 0.2875252396256691 1102 74-75 0.17667636563481537 1102 76-77 0.09386959669328476 1102 78-79 0.16948930178068267 1102 80-81 0.018059999215658706 1102 82-83 0.031079706776782956 1102 84-85 -0.12087562148727216 1102 86-87 -0.09702067307669893 1102 88-89 -0.06357911493293145 1102 90-91 -0.03089452035790785 1102 92-93 -0.12462723773602491 1102 94-95 -0.16568941630933054 1102 96-97 -0.27851453417096295 1102 98-99 -0.12033537982548026 1102 100-101 -0.08317047407662059 1103 1 0.10021481286847589 1103 2 0.20812420110588903 1103 3 0.3256727965935511 1103 4 0.23415794377300614 1103 5 0.28456086930163593 1103 6 0.21570285477718443 1103 7 0.24608482229347572 1103 8 0.17214747179787793 1103 9 0.29280816927946063 1103 10-11 0.3794247609417525 1103 12-13 0.35924321502160694 1103 14-15 0.3530480847548958 1103 16-17 0.4144538912923821 1103 18-19 0.4455104896368809 1103 20-21 0.4663188848529387 1103 22-23 0.4680740290428673 1103 24-25 0.5604340608165685 1103 26-27 0.467669261470256 1103 28-29 0.4968959389397085 1103 30-31 0.4520225761846888 1103 32-33 0.4325059040831647 1103 34-35 0.4324621614655513 1103 36-37 0.5120094399912105 1103 38-39 0.46771208411693976 1103 40-41 0.4350152441398123 1103 42-43 0.4097642182914001 1103 44-45 0.5204378724451288 1103 46-47 0.43208429264972636 1103 48-49 0.2632429533105878 1103 50-51 0.24360993029790734 1103 52-53 0.1488181387267815 1103 54-55 0.2913189628036079 1103 56-57 0.29621572501594784 1103 58-59 0.36587844046206186 1103 60-61 0.37915042030892465 1103 62-63 0.36861392549933925 1103 64-65 0.10916337179403968 1103 66-67 0.271922243649513 1103 68-69 0.263170881922683 1103 70-71 0.28459681626666367 1103 72-73 0.2088409873717545 1103 74-75 0.29985792913335985 1103 76-77 0.2888021548745776 1103 78-79 0.22531662248137607 1103 80-81 0.3276091818760207 1103 82-83 0.3933924990071631 1103 84-85 0.38923890132218375 1103 86-87 0.4570746076851222 1103 88-89 0.4790042328315316 1103 90-91 0.41862515888448826 1103 92-93 0.4234005010768591 1103 94-95 0.28713981051790327 1103 96-97 0.2228443760956118 1103 98-99 0.16443963606905143 1103 100-101 0.538881124339678 1104 1 0.1730874699504028 1104 2 0.23730415865779975 1104 3 0.15744133084549006 1104 4 0.2565147442823985 1104 5 0.2180137040865162 1104 6 0.24093528669552455 1104 7 0.14267050721190344 1104 8 0.09626938361574133 1104 9 0.299087902268937 1104 10-11 0.3183826428859433 1104 12-13 0.3340021846214114 1104 14-15 0.3198195881000103 1104 16-17 0.2815795324587427 1104 18-19 0.32616318768346986 1104 20-21 0.34168830180259135 1104 22-23 0.4025858977142889 1104 24-25 0.4553987449823609 1104 26-27 0.3819544352799653 1104 28-29 0.37759439586363186 1104 30-31 0.4705760064779909 1104 32-33 0.34599254062174367 1104 34-35 0.25808988428698854 1104 36-37 0.27883319219821345 1104 38-39 0.3303603836288289 1104 40-41 0.4342960372099469 1104 42-43 0.47670982967859743 1104 44-45 0.415038504366251 1104 46-47 0.5084887574600359 1104 48-49 0.6251704009656436 1104 50-51 0.6839422982585006 1104 52-53 0.49035738960012765 1104 54-55 0.41000777058596327 1104 56-57 0.5116709503283161 1104 58-59 0.6339631787250184 1104 60-61 0.567745633242474 1104 62-63 0.5208679165781476 1104 64-65 0.41243369595082413 1104 66-67 0.47806015402701973 1104 68-69 0.5464456982254688 1104 70-71 0.5920006175732411 1104 72-73 0.5598474658418233 1104 74-75 0.49055371379866486 1104 76-77 0.4286292461337773 1104 78-79 0.25714379090260664 1104 80-81 0.3820998549158361 1104 82-83 0.3809589447102901 1104 84-85 0.4295407334567898 1104 86-87 0.5983160370548717 1104 88-89 0.45822102927047226 1104 90-91 0.4966289413192655 1104 92-93 0.6532203501712566 1104 94-95 0.5484077471379685 1104 96-97 0.574998789277636 1104 98-99 0.3359468990945196 1104 100-101 0.6397643454416411 1105 1 0.1123566866020802 1105 2 0.11740980240059073 1105 3 0.17942581868422636 1105 4 0.015491770092019408 1105 5 0.18728880322004926 1105 6 0.22050755929144827 1105 7 0.13506203289663432 1105 8 0.20812579938891673 1105 9 0.28298662198369584 1105 10-11 0.1442510927104692 1105 12-13 0.29368142172462797 1105 14-15 0.3522624987804832 1105 16-17 0.3101455639344479 1105 18-19 0.4141823442839012 1105 20-21 0.3651326698233177 1105 22-23 0.21661761260796908 1105 24-25 0.4163524472521445 1105 26-27 0.32722471231684835 1105 28-29 0.3947857422727239 1105 30-31 0.3100614656591816 1105 32-33 0.19257957631678124 1105 34-35 0.33303528664047377 1105 36-37 0.32582490432844935 1105 38-39 0.42039740712493767 1105 40-41 0.24504630546302053 1105 42-43 0.15287056758827333 1105 44-45 0.17385210371219273 1105 46-47 0.4389592877934163 1105 48-49 0.4401378267042091 1105 50-51 0.28992131203639815 1105 52-53 0.14673648616528112 1105 54-55 0.1741619029315018 1105 56-57 0.10153612930134415 1105 58-59 0.14586965292388498 1105 60-61 0.2804996753986657 1105 62-63 0.24493394377225286 1105 64-65 0.1957717148534286 1105 66-67 0.18337350250027384 1105 68-69 0.08670680901162342 1105 70-71 -0.0585463603506895 1105 72-73 0.025814775614367846 1105 74-75 0.11704202307352318 1105 76-77 0.022763614907770346 1105 78-79 0.02695993903374827 1105 80-81 -0.09398124524905782 1105 82-83 -0.13921573292520506 1105 84-85 0.0901945351032154 1105 86-87 0.11126526207816312 1105 88-89 0.12308320749559343 1105 90-91 0.09580188149632818 1105 92-93 0.279645451431076 1105 94-95 0.20871887571091108 1105 96-97 0.3023419089436885 1105 98-99 0.4366924198523634 1105 100-101 0.7158281160152704 1106 1 0.07616770961971042 1106 2 0.15192364755064247 1106 3 0.16447823511595416 1106 4 0.09218937879371225 1106 5 0.166952069785836 1106 6 0.30610827098976046 1106 7 0.22178422509241358 1106 8 0.2370730570103632 1106 9 0.29913932091222506 1106 10-11 0.24936436209750212 1106 12-13 0.29998575753349144 1106 14-15 0.24532200813597882 1106 16-17 0.326363710007314 1106 18-19 0.35458849465422304 1106 20-21 0.47771120997678906 1106 22-23 0.3885894362518343 1106 24-25 0.4202671030302412 1106 26-27 0.23697825584292787 1106 28-29 0.33562546207301835 1106 30-31 0.24085562164450636 1106 32-33 0.36930512536390125 1106 34-35 0.3338142079054123 1106 36-37 0.21155927427580679 1106 38-39 0.390634208354939 1106 40-41 0.34040157748111 1106 42-43 0.4469844490564725 1106 44-45 0.28956043514799035 1106 46-47 0.28180229169418425 1106 48-49 0.2723343560292548 1106 50-51 0.22480777627805537 1106 52-53 0.11838462570489128 1106 54-55 0.04860248737880113 1106 56-57 0.14911648965876623 1106 58-59 0.2627066969814038 1106 60-61 0.314780588863222 1106 62-63 0.33989474279452736 1106 64-65 0.2493373382897488 1106 66-67 0.41236231977443083 1106 68-69 0.3567563340257536 1106 70-71 0.4399249404320713 1106 72-73 0.3344647256196218 1106 74-75 0.3766162815230061 1106 76-77 0.35302952507991847 1106 78-79 0.4430075788264105 1106 80-81 0.39111886023970754 1106 82-83 0.5127458769542486 1106 84-85 0.347782680376973 1106 86-87 0.4975917353478039 1106 88-89 0.60680126119777 1106 90-91 0.5585358192347556 1106 92-93 0.7358517167706999 1106 94-95 0.8443087463833443 1106 96-97 0.9415773430873919 1106 98-99 0.7693778694198237 1106 100-101 1.0744065753980827 1107 1 0.08097766580639032 1107 2 0.0910780159040776 1107 3 0.17234610010250506 1107 4 0.15545655095696276 1107 5 0.08219700946578001 1107 6 0.11994391977843577 1107 7 0.039174369822291055 1107 8 0.1594791030944478 1107 9 0.15524355909358434 1107 10-11 0.16730383672030058 1107 12-13 0.1963461792813206 1107 14-15 0.2184539024231711 1107 16-17 0.20247021552731326 1107 18-19 0.22926677671972584 1107 20-21 0.20387666915712543 1107 22-23 0.1789035169102604 1107 24-25 0.3238809381364689 1107 26-27 0.24487781788105423 1107 28-29 0.15656317793780516 1107 30-31 0.358261929015093 1107 32-33 0.3883749711020599 1107 34-35 0.27023163580104637 1107 36-37 0.22226552456314863 1107 38-39 0.1312057633066388 1107 40-41 0.17908107545692786 1107 42-43 0.16469350610346112 1107 44-45 0.3082523435189586 1107 46-47 0.4310640645074244 1107 48-49 0.4940823599002968 1107 50-51 0.5081606418485762 1107 52-53 0.3528827685717886 1107 54-55 0.49624866718446015 1107 56-57 0.4903352498585747 1107 58-59 0.3617515591606306 1107 60-61 0.26195295485965886 1107 62-63 0.32741709849655365 1107 64-65 0.4407391989352334 1107 66-67 0.35999336495186185 1107 68-69 0.3744314381048852 1107 70-71 0.3787191474674998 1107 72-73 0.25917601830819237 1107 74-75 0.33824971340275667 1107 76-77 0.3647461545810806 1107 78-79 0.36971471491784413 1107 80-81 0.2519796550704072 1107 82-83 0.4461295971772259 1107 84-85 0.5215619798036002 1107 86-87 0.5604020130739436 1107 88-89 0.4771768112915318 1107 90-91 0.5665398958753727 1107 92-93 0.3974212976528655 1107 94-95 0.46112137654471397 1107 96-97 0.638868929976006 1107 98-99 0.7075627648895164 1107 100-101 0.8605869750265143 1108 1 0.09116478788374494 1108 2 0.0644990090935984 1108 3 0.033101590108625345 1108 4 0.10483419717370879 1108 5 0.053678815741768915 1108 6 0.10979998060769702 1108 7 0.021504473247517808 1108 8 0.005961992553714879 1108 9 0.09051512613915946 1108 10-11 0.07522408727935925 1108 12-13 0.10336254950239265 1108 14-15 0.17793750418376675 1108 16-17 0.22191646046307056 1108 18-19 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58-59 -2.0743483438349486 2316 60-61 -2.141964663217884 2316 62-63 -2.482564850318994 2316 64-65 -2.8586913304506254 2316 66-67 -2.5850815016596123 2316 68-69 -2.7475007807875045 2316 70-71 -2.667762920533754 2316 72-73 -2.6173663525379176 2316 74-75 -2.730805150499794 2316 76-77 -2.7323133595295914 2316 78-79 -2.576627951670833 2316 80-81 -2.61150273802609 2316 82-83 -2.642691510259901 2316 84-85 -2.980954738657129 2316 86-87 -2.904143425985115 2316 88-89 -3.031705531748159 2316 90-91 -3.296447706786047 2316 92-93 -3.5662532101744944 2316 94-95 -3.751886352478568 2316 96-97 -4.058537622414537 2316 98-99 -4.431276014910932 2316 100-101 -4.9550862848353745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9624.0 3 2698.0 4 2750.0 5 3120.0 6 3628.0 7 4347.0 8 5378.0 9 6444.0 10 8095.0 11 9966.0 12 12650.0 13 17108.0 14 16760.0 15 15546.0 16 18897.0 17 24093.0 18 21489.0 19 16664.0 20 16856.0 21 18040.0 22 19986.0 23 22209.0 24 25089.0 25 28391.0 26 32736.0 27 38226.0 28 45081.0 29 52730.0 30 63226.0 31 76068.0 32 94277.0 33 119933.0 34 158645.0 35 223903.0 36 341705.0 37 530483.0 38 496847.0 39 80746.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09061544893039 19.275503795864278 14.803852014903265 22.830028740302062 2 14.852189501384949 20.597274695577898 40.822594963775835 23.727940839261326 3 19.081326104213716 24.892885014565998 30.362190526154475 25.663598355065808 4 12.714515329049677 17.14776375997106 37.95190023531518 32.18582067566408 5 13.569482146566457 37.47071106067449 34.54929436171835 14.4105124310407 6 30.992789339855488 36.47468066543879 17.94265027179789 14.589879722907828 7 27.59913657615681 33.07838789267682 21.513184216600216 17.809291314566156 8 25.271059371500947 35.38378591613574 20.274819657290124 19.07033505507318 9 25.821340305671193 17.6548081239867 21.26472312251756 35.25912844782455 10-11 24.480365424833213 26.744248477551885 27.791030596611744 20.984355501003158 12-13 25.824636788435697 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21.895040732802595 38-39 22.02559053200521 27.05859123563057 29.194307753292016 21.721510479072204 40-41 22.16639482253978 27.00530142863296 29.10527390090392 21.723029847923343 42-43 21.90564782011972 27.109529082022483 28.68399476789022 22.30082832996758 44-45 22.126798523241337 27.04053404629438 28.578259122244777 22.254408308219507 46-47 22.383101200516446 27.254181125792332 28.301513890127378 22.061203783563844 48-49 22.331348879181895 27.33187728413346 28.14387829747175 22.192895539212895 50-51 22.003646092909793 27.470012478406364 28.389423707473178 22.136917721210665 52-53 22.352345345265444 27.602442683099117 27.91705280151214 22.1281591701233 54-55 22.0082112179761 27.253944487160464 28.226558272893914 22.511286021969525 56-57 22.266193033045724 27.543589351709297 27.956426755729563 22.233790859515413 58-59 22.5309725231814 27.283894662518893 27.968427223232528 22.21670559106718 60-61 22.294491816510437 27.202320630165573 28.482339767839647 22.020847785484342 62-63 22.165665041324186 27.218457606248748 28.493860334193993 22.122017018233073 64-65 21.986488892220336 27.493155433957668 28.235760398024865 22.284595275797127 66-67 22.07424044416149 27.4439030405848 27.90835795826503 22.573498556988685 68-69 21.87445355285991 27.38494599392046 28.22951782582025 22.51108262739938 70-71 22.164934542615423 27.27584683043226 28.03632718984347 22.522891437108846 72-73 22.101322848988328 27.083623020833137 28.295681766647114 22.51937236353142 74-75 21.931284580987956 27.620190893237485 27.922043120568862 22.526481405205693 76-77 22.144495372019346 27.24079195699475 27.925269555679755 22.689443115306148 78-79 22.13303207698296 27.311721649322322 27.989866877261388 22.56537939643333 80-81 22.11538965831145 27.4302743323381 27.910159249866073 22.544176759484376 82-83 22.169584838035448 27.303240977079568 28.03330992251951 22.49386426236547 84-85 22.076568500913073 27.288158488601994 27.90991385617415 22.72535915431078 86-87 22.13865316140021 27.254533420682442 28.076288219841 22.530525198076347 88-89 22.16767338346214 27.157408862917453 27.841722723233413 22.833195030386992 90-91 22.094995814745474 27.09783189386937 28.05370392683122 22.75346836455394 92-93 22.269509686465373 27.248504869703044 27.94977733975968 22.5322081040719 94-95 22.176298886021257 27.047360400345717 27.917007421777495 22.859333291855535 96-97 21.9937530193927 26.980262494617097 28.08317239275323 22.942812093236974 98-99 22.080863067105536 27.084581116059347 27.707223134660087 23.127332682175027 100-101 22.209906389050797 26.526505990010314 28.24838687254593 23.01520074839296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2967.0 1 1708.5 2 520.0 3 564.0 4 504.5 5 540.5 6 797.0 7 1621.5 8 2755.0 9 3424.0 10 3303.5 11 3000.5 12 3225.5 13 3562.5 14 3741.5 15 4000.0 16 4388.5 17 4595.0 18 4616.5 19 4843.5 20 5382.0 21 6006.5 22 6842.5 23 8280.5 24 9998.5 25 12465.5 26 15935.0 27 19784.5 28 23197.0 29 27269.5 30 32077.0 31 36975.5 32 41978.5 33 46956.0 34 53334.0 35 59057.0 36 65067.5 37 72159.0 38 76547.0 39 78903.5 40 80908.5 41 83403.5 42 86115.5 43 90281.5 44 94975.5 45 97730.0 46 98860.5 47 105232.0 48 122417.5 49 122130.5 50 105803.5 51 97527.0 52 91612.5 53 87525.5 54 82929.0 55 76194.0 56 69663.0 57 62530.5 58 55399.0 59 48502.0 60 40716.0 61 32436.5 62 25463.0 63 20152.0 64 15389.5 65 11500.5 66 8297.0 67 6111.0 68 4428.0 69 2864.5 70 1981.0 71 1344.0 72 858.5 73 570.0 74 397.5 75 256.5 76 162.0 77 90.0 78 57.0 79 43.0 80 30.0 81 29.5 82 22.5 83 18.5 84 15.5 85 12.0 86 12.0 87 10.5 88 5.5 89 6.0 90 5.5 91 2.0 92 2.5 93 2.5 94 1.0 95 1.5 96 2.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2877308710564126 2 0.08955269472030243 3 0.09394837607512592 4 0.10963127311225042 5 0.11100957997774592 6 0.08616280486192159 7 0.09581095292039012 8 0.09223480537748285 9 0.09748727208112788 10-11 0.0902977254584081 12-13 0.09480516142394745 14-15 0.1035778983651418 16-17 0.09266319805189363 18-19 0.10314950569073103 20-21 0.10536597213659543 22-23 0.11812462352665515 24-25 0.13198219525542074 26-27 0.13535345934534895 28-29 0.12686010893094424 30-31 0.11866477081178177 32-33 0.11302116297063126 34-35 0.38808651147924733 36-37 0.592429817173182 38-39 0.5743814475425719 40-41 0.5370926591003828 42-43 0.5649754344739878 44-45 0.5874753627647793 46-47 0.5749774721330566 48-49 0.5977009096452798 50-51 0.5901760991904124 52-53 0.5927464552368769 54-55 0.5835453256212718 56-57 0.5542656176137187 58-59 0.5493856662791265 60-61 0.4947749131759804 62-63 0.5727237541502869 64-65 0.6063805177442108 66-67 0.6181706291747332 68-69 0.6202939667783344 70-71 0.6159169111919635 72-73 0.6089881253275806 74-75 0.6057099900799158 76-77 0.5903809826433913 78-79 0.5657390909805461 80-81 0.5611199004042909 82-83 0.5758528832503307 84-85 0.5855382828457045 86-87 0.5762626501562889 88-89 0.588369399650506 90-91 0.6018544560102188 92-93 0.6099752910555707 94-95 0.6093978922335388 96-97 0.6066040269656425 98-99 0.6104409352668868 100-101 0.5886301604088431 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2684453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.289984687170865 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52959148619989 41.76029956700263 2 13.472174760345435 14.89752672404778 3 4.735758593546459 7.855200603579647 4 2.125044676753559 4.69974750549033 5 1.1582098042221518 3.2018701169986974 6 0.7032433963228578 2.3329389968426906 7 0.4819208301593925 1.865177672395904 8 0.31111177777866145 1.3761092343504557 9 0.22638601167601688 1.1265191207060992 >10 1.1367570773601203 11.165757411636692 >50 0.07863804523102585 2.982198379456571 >100 0.03730794782950117 3.60428152069841 >500 0.002435111100023675 0.8721876205304827 >1k 0.0012175555499787704 1.3594996866863729 >5k 2.0292592499646174E-4 0.9006858395771209 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9230 0.3438316856357701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7526 0.2803550667491664 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7327 0.2729420109050149 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.16968075060356802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4333 0.16141090941059502 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3209 0.11954018192905594 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.725153690528387E-5 0.0 0.0 3.725153690528387E-5 0.0 3 3.725153690528387E-5 0.0 0.0 3.725153690528387E-5 0.0 4 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 0.0 5 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 0.0 6 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 3.725153690528387E-5 7 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 3.725153690528387E-5 8 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 3.725153690528387E-5 9 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 7.450307381056774E-5 10-11 3.725153690528387E-5 0.0 0.0 7.450307381056774E-5 7.450307381056774E-5 12-13 3.725153690528387E-5 0.0 0.0 1.117546107158516E-4 7.450307381056774E-5 14-15 3.725153690528387E-5 0.0 0.0 1.117546107158516E-4 2.607607583369871E-4 16-17 3.725153690528387E-5 0.0 0.0 1.3038037916849354E-4 3.725153690528387E-4 18-19 3.725153690528387E-5 0.0 0.0 1.8625768452641936E-4 3.725153690528387E-4 20-21 3.725153690528387E-5 0.0 0.0 2.9801229524227096E-4 3.9114113750548065E-4 22-23 3.725153690528387E-5 0.0 0.0 5.40147285126616E-4 4.283926744107645E-4 24-25 3.725153690528387E-5 0.0 0.0 7.264049696530355E-4 4.842699797686903E-4 26-27 3.725153690528387E-5 0.0 0.0 0.0010244172648953063 4.842699797686903E-4 28-29 7.450307381056774E-5 0.0 0.0 0.0031105033315912034 4.842699797686903E-4 30-31 7.450307381056774E-5 0.0 0.0 0.008623730793573215 4.842699797686903E-4 32-33 7.450307381056774E-5 0.0 0.0 0.019408050727652896 5.58773053579258E-4 34-35 9.312884226320966E-5 0.0 0.0 0.03624574540884121 5.58773053579258E-4 36-37 1.117546107158516E-4 0.0 0.0 0.06306685198064559 5.58773053579258E-4 38-39 1.117546107158516E-4 0.0 0.0 0.1013614319192774 5.58773053579258E-4 40-41 1.117546107158516E-4 0.0 0.0 0.14388406129665895 5.960245904845419E-4 42-43 1.3038037916849354E-4 0.0 0.0 0.18638806490558785 5.960245904845419E-4 44-45 1.4900614762113548E-4 0.0 0.0 0.24289864639090347 5.960245904845419E-4 46-47 2.0488345297906127E-4 0.0 0.0 0.31328542537343734 6.146503589371838E-4 48-49 2.235092214317032E-4 0.0 0.0 0.3897442048715325 6.705276642951097E-4 50-51 2.235092214317032E-4 0.0 0.0 0.47234948795899945 6.705276642951097E-4 52-53 2.235092214317032E-4 0.0 0.0 0.5541166114660976 6.705276642951097E-4 54-55 2.235092214317032E-4 0.0 0.0 0.65417423959369 7.264049696530355E-4 56-57 2.235092214317032E-4 3.725153690528387E-5 0.0 0.7747388387876413 7.450307381056774E-4 58-59 2.235092214317032E-4 3.725153690528387E-5 0.0 0.9059760033049564 7.450307381056774E-4 60-61 2.235092214317032E-4 3.725153690528387E-5 0.0 1.0532127029230907 7.450307381056774E-4 62-63 2.235092214317032E-4 3.725153690528387E-5 0.0 1.2006542859942044 7.450307381056774E-4 64-65 2.235092214317032E-4 3.725153690528387E-5 0.0 1.355844188741617 7.450307381056774E-4 66-67 2.235092214317032E-4 3.725153690528387E-5 0.0 1.5415244744460046 7.450307381056774E-4 68-69 2.235092214317032E-4 3.725153690528387E-5 0.0 1.7557394374198392 7.450307381056774E-4 70-71 2.4213498988434514E-4 3.725153690528387E-5 0.0 1.995769715469036 7.822822750109613E-4 72-73 2.607607583369871E-4 3.725153690528387E-5 0.0 2.238482104175413 9.312884226320967E-4 74-75 2.607607583369871E-4 3.725153690528387E-5 0.0 2.4925189601009965 9.312884226320967E-4 76-77 2.607607583369871E-4 3.725153690528387E-5 0.0 2.7673794251566335 9.312884226320967E-4 78-79 2.607607583369871E-4 3.725153690528387E-5 0.0 3.092156949665351 9.312884226320967E-4 80-81 3.3526383214755483E-4 3.725153690528387E-5 0.0 3.44345756845063 9.499141910847386E-4 82-83 3.3526383214755483E-4 3.725153690528387E-5 0.0 3.807758973615854 9.685399595373806E-4 84-85 3.5388960060019677E-4 3.725153690528387E-5 0.0 4.196478761222491 9.685399595373806E-4 86-87 3.725153690528387E-4 3.725153690528387E-5 0.0 4.603712562671054 9.685399595373806E-4 88-89 3.725153690528387E-4 3.725153690528387E-5 0.0 5.055741337248222 9.685399595373806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9035 0.0 21.93544 1 GAGTACT 8085 0.0 21.68601 12-13 TACATGG 9290 0.0 20.173971 2 GTACTTT 8900 0.0 19.700521 14-15 ACATGGG 9210 0.0 19.475632 3 GTATCAA 20525 0.0 19.450338 1 AGTACTT 8620 0.0 19.406786 12-13 TTTGCGT 495 0.0 19.122152 3 TACGTTG 205 1.641366E-5 18.4692 5 AGAGTAC 15240 0.0 17.512877 10-11 CATGGGG 4600 0.0 17.284761 4 ATCAACG 22935 0.0 17.271841 3 GGTATCA 16510 0.0 17.230682 1 TATCAAC 23125 0.0 17.211796 2 TCAACGC 23010 0.0 17.194977 4 CAACGCA 22965 0.0 17.187452 5 AACGCAG 23250 0.0 16.974236 6 ACTTTTT 11075 0.0 16.303276 16-17 ACGCAGA 24945 0.0 15.631147 7 CGCAGAG 24755 0.0 15.54114 8 >>END_MODULE