FastQCFastQC Report
Thu 26 May 2016
SRR938896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684453
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93120.3468863116620034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80740.300768908973262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80450.2996886144030087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.19203167274673835No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40310.15016094526519927No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28470.10605512556934318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90550.022.41851
GAGTACT83550.021.58274812-13
TACATGG93150.021.2425482
ACATGGG92850.020.4423833
GTACTTT90850.019.79629314-15
AGTACTT89950.019.361312-13
TTTGCGT5850.018.6562963
GTATCAA217400.018.2587411
GGTATCA174400.017.8971
AGAGTAC159000.017.14598710-11
GTTCGCG1952.2944296E-417.0340087
CATGGGG45650.016.9433884
CTTTGCG6600.016.536262
TACTTTT106400.016.18954314-15
ACTTTTT112350.016.17644916-17
CAACGCA244600.015.9854245
ATCAACG245250.015.9430583
TATCAAC247500.015.9323292
TCAACGC245450.015.9300674
AACGCAG248750.015.7187336
Produced by FastQC (version 0.11.5)