##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938896_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2684453 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18687568752368 33.0 31.0 34.0 30.0 34.0 2 32.42641126516277 34.0 31.0 34.0 31.0 34.0 3 32.52174912356447 34.0 31.0 34.0 30.0 34.0 4 36.082655945177656 37.0 35.0 37.0 35.0 37.0 5 35.99886420063976 37.0 35.0 37.0 35.0 37.0 6 36.03608705386162 37.0 35.0 37.0 35.0 37.0 7 35.9811160783966 37.0 35.0 37.0 35.0 37.0 8 35.94724623601158 37.0 35.0 37.0 35.0 37.0 9 37.639246803725 39.0 37.0 39.0 35.0 39.0 10-11 37.560310983280395 39.0 37.0 39.0 35.0 39.0 12-13 37.49132039190107 39.0 37.0 39.0 34.5 39.0 14-15 38.762398149641655 40.0 38.0 41.0 34.0 41.0 16-17 38.75663924829378 40.0 38.0 41.0 34.5 41.0 18-19 38.85849426307706 40.0 38.0 41.0 35.0 41.0 20-21 38.862939116460595 40.0 38.0 41.0 34.5 41.0 22-23 38.80802997854684 40.0 38.0 41.0 34.0 41.0 24-25 38.74889390874044 40.0 38.0 41.0 34.0 41.0 26-27 38.60018186200317 40.0 38.0 41.0 34.0 41.0 28-29 38.42792013866512 40.0 38.0 41.0 34.0 41.0 30-31 38.27775006677338 40.0 38.0 41.0 34.0 41.0 32-33 38.1265367655906 40.0 38.0 41.0 33.0 41.0 34-35 38.0912644028411 40.0 38.0 41.0 33.0 41.0 36-37 37.99696195090769 40.0 37.5 41.0 33.0 41.0 38-39 37.88016739350624 40.0 37.0 41.0 33.0 41.0 40-41 37.74289175485657 40.0 37.0 41.0 33.0 41.0 42-43 37.58610804510268 40.0 37.0 41.0 33.0 41.0 44-45 37.35166438004316 40.0 37.0 41.0 32.0 41.0 46-47 37.063207290274775 40.0 37.0 41.0 31.0 41.0 48-49 36.78998049137012 40.0 36.0 41.0 30.5 41.0 50-51 36.530840361146204 39.0 36.0 41.0 30.0 41.0 52-53 36.31538697082795 39.0 35.5 41.0 30.0 41.0 54-55 36.07156597638327 39.0 35.0 40.5 29.0 41.0 56-57 35.797636799750265 39.0 35.0 40.0 28.5 41.0 58-59 35.493780669655976 38.0 35.0 40.0 28.0 41.0 60-61 35.08920979432309 38.0 34.0 40.0 27.5 41.0 62-63 34.78145566340703 37.5 34.0 40.0 27.0 41.0 64-65 34.525169000910054 37.0 34.0 40.0 26.0 41.0 66-67 34.07077773386236 36.5 33.5 39.0 26.0 41.0 68-69 33.653425669959574 36.0 33.0 39.0 26.0 40.0 70-71 33.189802354520644 35.5 32.5 38.5 25.0 40.0 72-73 32.67147962732072 35.0 32.0 38.0 23.5 40.0 74-75 32.199280263055456 35.0 31.5 37.0 22.5 39.0 76-77 30.211066649332285 33.5 29.5 35.5 20.0 37.5 78-79 31.189349003316504 34.0 31.0 36.0 20.0 38.0 80-81 31.171940428832244 34.0 31.0 36.0 20.0 37.5 82-83 30.846675281705437 34.0 31.0 35.0 19.5 37.0 84-85 30.433694313143125 34.0 30.5 35.0 18.0 36.5 86-87 29.853206034897987 34.0 30.0 35.0 10.5 36.0 88-89 29.275110422868273 34.0 29.0 35.0 6.0 36.0 90-91 28.8055989432484 34.0 29.0 35.0 2.0 35.0 92-93 28.442089692015468 34.0 29.0 35.0 2.0 35.0 94-95 28.038616619475178 33.0 28.5 35.0 2.0 35.0 96-97 27.408829284774214 33.0 27.0 35.0 2.0 35.0 98-99 26.501822717700776 33.0 25.5 34.5 2.0 35.0 100-101 22.07559528887263 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2156100046952858 1101 2 0.19518046112791865 1101 3 0.15385058997572543 1101 4 0.1725869120279384 1101 5 0.16678623840108742 1101 6 0.1616913571850418 1101 7 0.1742292549277238 1101 8 0.1961031317976918 1101 9 0.2390378954048984 1101 10-11 0.20934486716520695 1101 12-13 0.2504005996852001 1101 14-15 0.24630287065996015 1101 16-17 0.31109420134011856 1101 18-19 0.27564636323405267 1101 20-21 0.25972973824081436 1101 22-23 0.252354859351847 1101 24-25 0.29454962665980133 1101 26-27 0.29237883528554676 1101 28-29 0.3457086998680339 1101 30-31 0.33689586662427473 1101 32-33 0.39078289808522015 1101 34-35 0.4545753931561265 1101 36-37 0.39669854494378143 1101 38-39 0.4296496136114598 1101 40-41 0.37177432747392203 1101 42-43 0.39779081755092704 1101 44-45 0.407228180221864 1101 46-47 0.36844121980166733 1101 48-49 0.2520551582545423 1101 50-51 0.3456131527984496 1101 52-53 0.3770318777078998 1101 54-55 0.4794420291948285 1101 56-57 0.14857393290611753 1101 58-59 0.37208613497866594 1101 60-61 0.5128209740216505 1101 62-63 0.39245976417052475 1101 64-65 0.4332452587759903 1101 66-67 0.5010990018219488 1101 68-69 0.46684427701372755 1101 70-71 0.46166523841650076 1101 72-73 0.3943586559320309 1101 74-75 0.32228852938479946 1101 76-77 0.23213842360456383 1101 78-79 0.4006036251054397 1101 80-81 0.5332782574625305 1101 82-83 0.4547081398797843 1101 84-85 0.41218333407490704 1101 86-87 0.607244475965981 1101 88-89 0.5371577773757927 1101 90-91 0.5254217931886878 1101 92-93 0.6375255971480875 1101 94-95 0.45480196644897575 1101 96-97 0.30731034265711443 1101 98-99 0.18259967985455816 1101 100-101 0.9814109749944961 1102 1 0.09579369258383252 1102 2 0.05272362650594431 1102 3 0.10591805074400185 1102 4 0.11635603732341337 1102 5 0.06272458303519102 1102 6 0.09548388998927493 1102 7 0.13309297316765623 1102 8 0.12987166780473558 1102 9 0.16056472352039464 1102 10-11 0.16049786770649632 1102 12-13 0.10466030456712616 1102 14-15 0.2534318938445068 1102 16-17 0.27826536776064614 1102 18-19 0.17216731365302707 1102 20-21 0.1938905763183314 1102 22-23 0.21253004812158593 1102 24-25 0.18121032349034039 1102 26-27 0.17192782307633792 1102 28-29 0.2902984245002287 1102 30-31 0.18258081723035957 1102 32-33 0.15662974505794836 1102 34-35 0.22494426949243262 1102 36-37 0.24008815633539626 1102 38-39 0.27525771371515617 1102 40-41 0.23792231906350736 1102 42-43 0.19151890528564763 1102 44-45 0.26995996953849044 1102 46-47 0.3771904824317289 1102 48-49 0.23960370620530824 1102 50-51 0.2477786663025725 1102 52-53 0.21966604562351222 1102 54-55 0.35721935471229926 1102 56-57 0.32989798504782186 1102 58-59 0.17031605224135404 1102 60-61 0.1644881742879818 1102 62-63 0.1724496855157085 1102 64-65 0.14820112593215384 1102 66-67 0.20443783720573805 1102 68-69 0.334519406550875 1102 70-71 0.2918134778717416 1102 72-73 0.35532163829044094 1102 74-75 0.34680216898206595 1102 76-77 0.2753189894794197 1102 78-79 0.2438008064841668 1102 80-81 0.37645706154721026 1102 82-83 0.4138289883579347 1102 84-85 0.4120431502533677 1102 86-87 0.506193659254734 1102 88-89 0.6440141483431923 1102 90-91 0.6959905719582657 1102 92-93 0.646943580651655 1102 94-95 0.5960726302732873 1102 96-97 0.48137840121902187 1102 98-99 0.7678674385476256 1102 100-101 1.235686049976895 1103 1 0.07067454826208319 1103 2 0.1233419169816159 1103 3 0.12487827286876296 1103 4 0.09848294341191632 1103 5 0.03965080409899713 1103 6 0.0960178671589702 1103 7 0.07621930677905908 1103 8 0.11868416892701106 1103 9 0.11486212126049367 1103 10-11 0.17432938292036226 1103 12-13 0.025102575837266272 1103 14-15 0.13365125817885826 1103 16-17 0.13618125593252017 1103 18-19 0.14050211749059827 1103 20-21 0.15078968613318722 1103 22-23 0.0859624698031638 1103 24-25 0.194595893963303 1103 26-27 0.11750742341367726 1103 28-29 0.08898682500653621 1103 30-31 0.16868911735687675 1103 32-33 0.2128593366757343 1103 34-35 0.20421282272625518 1103 36-37 0.09824805487821209 1103 38-39 0.19245754595898035 1103 40-41 0.22939367344812212 1103 42-43 0.2256329551395453 1103 44-45 0.16837899233440368 1103 46-47 0.2910061259388925 1103 48-49 0.19739287941873584 1103 50-51 0.033528582378828276 1103 52-53 0.17262454392571414 1103 54-55 0.30450383200053466 1103 56-57 0.15685719734855752 1103 58-59 0.1097123826053732 1103 60-61 0.13800380449517036 1103 62-63 0.15147925010746377 1103 64-65 0.2655014840575092 1103 66-67 0.30948799210325006 1103 68-69 0.27785787016357943 1103 70-71 0.2218186426437896 1103 72-73 0.25482466974076345 1103 74-75 0.2821528119611898 1103 76-77 0.0760368532298692 1103 78-79 0.3071836226149358 1103 80-81 0.308304059512043 1103 82-83 0.38035383937236844 1103 84-85 0.4103973166600703 1103 86-87 0.33032358385879945 1103 88-89 0.4966417755823862 1103 90-91 0.6464488579782888 1103 92-93 0.7395995000069497 1103 94-95 0.5603860184154961 1103 96-97 0.3591037923888756 1103 98-99 0.4261281368844614 1103 100-101 0.8695213634438623 1104 1 0.03910346506123119 1104 2 0.02039416300227259 1104 3 -0.016794949828771166 1104 4 0.0511601891521849 1104 5 -0.008549743003712251 1104 6 0.03460676207760116 1104 7 -0.030251581677390504 1104 8 -0.011809232897093125 1104 9 0.07786741478500403 1104 10-11 0.017578161524710367 1104 12-13 -0.005080114237408395 1104 14-15 0.09312122981283721 1104 16-17 0.031607566419658895 1104 18-19 0.11394948214563527 1104 20-21 0.10854527002468473 1104 22-23 0.07784004476809514 1104 24-25 0.09703520101837881 1104 26-27 0.14419314830382746 1104 28-29 0.1484753843152049 1104 30-31 -0.009566981920144713 1104 32-33 0.026276841420227015 1104 34-35 0.0650671798461886 1104 36-37 0.030494260966321463 1104 38-39 0.15852217932644663 1104 40-41 0.04270575332817117 1104 42-43 0.15590592179672313 1104 44-45 0.2196358885037739 1104 46-47 0.2678294788772675 1104 48-49 0.18549624423646804 1104 50-51 0.03282866595607459 1104 52-53 -0.01521579707063836 1104 54-55 0.08443899795199172 1104 56-57 0.09597142212363963 1104 58-59 0.06464796352702962 1104 60-61 0.11920774747482454 1104 62-63 0.17967793395654041 1104 64-65 0.13368384125877952 1104 66-67 0.2188610401544011 1104 68-69 0.18919922286477941 1104 70-71 0.1662924880807708 1104 72-73 0.1338692273728057 1104 74-75 0.2870944716356689 1104 76-77 0.04211427216599972 1104 78-79 0.22394052349477533 1104 80-81 0.2876861711107672 1104 82-83 0.31808527872682646 1104 84-85 0.24827443672792882 1104 86-87 0.28330192600271076 1104 88-89 0.31808899129676504 1104 90-91 0.3540131443409358 1104 92-93 0.3356126157573165 1104 94-95 0.4803100018543702 1104 96-97 0.4874522842226341 1104 98-99 0.5188387626789819 1104 100-101 1.099127895418924 1105 1 -0.04820879225736974 1105 2 -0.04477714668376365 1105 3 -0.008515207845199768 1105 4 -0.03768284872806049 1105 5 -0.01271421504842607 1105 6 -8.596833198950549E-4 1105 7 -0.055487036976117565 1105 8 0.026848237571847733 1105 9 -0.10409213237565496 1105 10-11 -0.07206415658244225 1105 12-13 -0.0712136951971516 1105 14-15 -0.04817168832052232 1105 16-17 -0.012766709790504649 1105 18-19 -0.057548013699395995 1105 20-21 -0.00702989606144655 1105 22-23 -0.15256282178432912 1105 24-25 -0.05707544624475247 1105 26-27 -0.0022328266924986906 1105 28-29 0.03252975104564371 1105 30-31 -0.0019494364201761982 1105 32-33 -0.0773269007845343 1105 34-35 -0.016616516216011235 1105 36-37 -0.014643808996169128 1105 38-39 0.005322859563698046 1105 40-41 0.014151986094191216 1105 42-43 -0.002643800414574571 1105 44-45 0.03169472957792152 1105 46-47 0.11514482185005193 1105 48-49 0.08639398673064846 1105 50-51 0.1948046109159023 1105 52-53 0.1842838654087231 1105 54-55 0.15254400134170965 1105 56-57 0.16035535297712045 1105 58-59 0.016212844486240385 1105 60-61 0.1276104221535519 1105 62-63 0.11861057337085157 1105 64-65 -0.03222848987748961 1105 66-67 0.01257424470788493 1105 68-69 -0.022131368987210465 1105 70-71 0.02097464900823809 1105 72-73 -0.19070138991333607 1105 74-75 0.09978016785780142 1105 76-77 -0.13029825222278646 1105 78-79 0.005770403512755706 1105 80-81 -0.08277767412234383 1105 82-83 0.13666920438433294 1105 84-85 -0.00852840753115558 1105 86-87 0.006246682197566855 1105 88-89 0.08899331874534155 1105 90-91 0.184879163813644 1105 92-93 0.22700586119353616 1105 94-95 0.21340272866555665 1105 96-97 0.14059499979280687 1105 98-99 0.40514162210508076 1105 100-101 0.8598050789510516 1106 1 -0.07759728106418606 1106 2 -0.1086864979493285 1106 3 -0.09849844363793636 1106 4 -0.03124811986684506 1106 5 -0.04500643755038425 1106 6 -0.010840014806099418 1106 7 0.013185777998643289 1106 8 -0.0016839963640293831 1106 9 -0.057223384403869204 1106 10-11 -0.019674791614505693 1106 12-13 0.08253740000424159 1106 14-15 0.013140041440337313 1106 16-17 -0.06818776692944795 1106 18-19 -0.12494861925018341 1106 20-21 -0.1238348609935187 1106 22-23 0.032092644111976654 1106 24-25 0.08419529384071467 1106 26-27 0.06287025470799534 1106 28-29 0.07718489615069757 1106 30-31 0.003985774630393735 1106 32-33 0.020331122562765813 1106 34-35 0.013971144720237305 1106 36-37 -0.1210968831072563 1106 38-39 0.02566560783393612 1106 40-41 0.05881636826885028 1106 42-43 0.15712127154721145 1106 44-45 0.018792476678129333 1106 46-47 0.15572047602791628 1106 48-49 0.03263058975748123 1106 50-51 0.053672498479713227 1106 52-53 0.1388499422491094 1106 54-55 0.09815842686614928 1106 56-57 0.04971144186723109 1106 58-59 0.07997597795229439 1106 60-61 0.034017353879718826 1106 62-63 0.0427179502235262 1106 64-65 -0.009931277925836923 1106 66-67 0.028839865022213473 1106 68-69 0.007555999885788367 1106 70-71 0.03938097810511465 1106 72-73 0.12694582391437592 1106 74-75 0.04549090236380238 1106 76-77 -0.08328579263317692 1106 78-79 0.005934021373612097 1106 80-81 0.27443863971690874 1106 82-83 0.4107113632292787 1106 84-85 0.12826865505207152 1106 86-87 0.1542055619143099 1106 88-89 0.23965357588817326 1106 90-91 0.4996778515482809 1106 92-93 0.5799610933418613 1106 94-95 0.7274371312362398 1106 96-97 0.5348490985455321 1106 98-99 0.7675253961397175 1106 100-101 0.9579447904165015 1107 1 -0.11861840586404782 1107 2 -0.11214661663336756 1107 3 -0.09021052132826668 1107 4 -0.12510166178391557 1107 5 -0.1311695894138154 1107 6 -0.16119626396548625 1107 7 -0.040246313067271444 1107 8 -0.12618958661024493 1107 9 -0.10952580397403011 1107 10-11 -0.08635410854589765 1107 12-13 -0.06614418826362822 1107 14-15 -0.08592273956757879 1107 16-17 -0.04835433636326769 1107 18-19 -0.08935418673734574 1107 20-21 -0.03522903724569204 1107 22-23 0.07295185694568573 1107 24-25 0.012049234969303768 1107 26-27 -0.0046519610701167835 1107 28-29 -0.037166358010253475 1107 30-31 -0.058141874250793535 1107 32-33 -0.10311832763616735 1107 34-35 -0.0906059238050787 1107 36-37 -0.09175862045405836 1107 38-39 -0.11608860409027244 1107 40-41 -0.1662490461643884 1107 42-43 -0.14813347137399546 1107 44-45 -0.2721042157773752 1107 46-47 -0.29650949721806086 1107 48-49 -0.4127564612259391 1107 50-51 -0.22659582991202143 1107 52-53 -0.24826743364622672 1107 54-55 -0.20548504926668443 1107 56-57 -0.14738996271193372 1107 58-59 -0.18488258609131236 1107 60-61 -0.2239770137541086 1107 62-63 -0.30141222699725034 1107 64-65 -0.34516893299509377 1107 66-67 -0.16601454699319618 1107 68-69 -0.05382334157512503 1107 70-71 -0.2354653697234781 1107 72-73 -0.05120759723978807 1107 74-75 -0.07889196889220074 1107 76-77 -0.36134810618098 1107 78-79 -0.12016034788790364 1107 80-81 0.05453042124255347 1107 82-83 -0.07248984194402652 1107 84-85 -0.22352713997709017 1107 86-87 -0.17997523921010838 1107 88-89 -0.029366368410681076 1107 90-91 0.16463851722062728 1107 92-93 0.20961823975624938 1107 94-95 0.1942838000331797 1107 96-97 0.1476692566138702 1107 98-99 0.09354392092467378 1107 100-101 0.8479585200943376 1108 1 -0.04238598881710942 1108 2 -0.10529151073848197 1108 3 -0.08615017271078784 1108 4 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4933.0 13 6814.0 14 8382.0 15 11229.0 16 14721.0 17 21172.0 18 21096.0 19 14888.0 20 13655.0 21 14458.0 22 15843.0 23 17706.0 24 20320.0 25 24208.0 26 28697.0 27 35631.0 28 42977.0 29 52345.0 30 63733.0 31 77533.0 32 96818.0 33 123188.0 34 165556.0 35 236483.0 36 372824.0 37 614838.0 38 512907.0 39 43520.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.781031999850384 20.062166863042137 15.413678954160353 22.74312218294713 2 15.219636924170398 21.199551640501806 40.0358285281955 23.54498290713229 3 19.092865473897287 25.029419401270946 30.55095395598284 25.326761168848922 4 13.045264715008981 17.762501336398888 37.41920607289455 31.77302787569758 5 13.482312718131844 37.65652688472059 34.61381001570903 14.247350381438533 6 30.99912719649031 36.98228279653248 17.546256164663713 14.4723338423135 7 27.52557038622021 33.19335447482224 21.37165374100422 17.909421397953327 8 25.348776827159945 35.698669337850205 19.954791534811747 18.9977623001781 9 25.64485204248314 17.54905002993161 21.094353300281284 35.71174462730396 10-11 24.502719920967138 26.974508400780344 27.60277419645641 20.919997481796106 12-13 25.767819365807487 24.430898957813753 27.94548833598502 21.855793340393742 14-15 22.467392361171903 25.79673724313324 26.659941432348255 25.075928963346605 16-17 21.669882174217054 28.981123064595383 27.317912804673846 22.03108195651372 18-19 21.56985054310878 27.92332367152638 29.282837136653168 21.223988648711675 20-21 22.460031894765898 26.80102799341244 29.16759950723667 21.571340604584993 22-23 22.073025677856904 26.811309417598295 29.10768041012452 22.00798449442028 24-25 22.235237495310965 26.992240132347263 29.128429516180766 21.644092856161013 26-27 22.08110926136535 26.92515011438084 29.352087743760087 21.641652880493716 28-29 21.627962940681027 27.410258253729904 29.278739467593585 21.68303933799549 30-31 21.95547845315228 26.9421181894412 29.142324339446436 21.960079017960084 32-33 21.795762488670874 27.1028585711875 29.143516388627404 21.957862551514218 34-35 22.325158970096915 26.99830892585034 28.992878638840843 21.68365346521191 36-37 22.22621257153757 26.766505703260567 29.17654216031717 21.830739564884695 38-39 21.95996160349555 27.115584618075676 29.2612771103594 21.663176668069372 40-41 22.103312104971707 26.99950696259342 29.168294137138908 21.728886795295974 42-43 21.903235113391666 27.121876134433524 28.7446387684259 22.230249983748905 44-45 22.11466626683194 27.07333086851367 28.592286852856784 22.21971601179761 46-47 22.34294239591424 27.318216614607486 28.309482965752697 22.029358023725575 48-49 22.360595512183057 27.389064147512027 28.14786144848163 22.10247889182328 50-51 21.957482348260605 27.537756506203532 28.334589958866623 22.17017118666924 52-53 22.327472300688445 27.664648999256087 27.884544821607975 22.12333387844749 54-55 22.001521352767213 27.314726687336304 28.104645527412845 22.579106432483638 56-57 22.246468833687906 27.583552403413286 27.912837363887537 22.25714139901127 58-59 22.50538844886431 27.30982482046575 27.922278935765515 22.262507794904423 60-61 22.302027353741227 27.313583068902908 28.369075833356305 22.01531374399956 62-63 22.164403697885565 27.304184502392108 28.41599387286721 22.115417926855116 64-65 21.94649400789064 27.578268447840465 28.133690104516873 22.341547439752024 66-67 22.013442444934313 27.52002071220558 27.853576627244557 22.612960215615548 68-69 21.82935717581214 27.529521067398203 28.106485014415718 22.534636742373937 70-71 22.129484503823406 27.381095400272493 27.91815090786669 22.571269188037412 72-73 22.11903132593493 27.145977225155367 28.12751797107269 22.60747347783701 74-75 21.945737176251548 27.740195116100004 27.65431169776487 22.659756009883576 76-77 22.040727418937635 27.288094280321445 27.877784547466806 22.793393753274113 78-79 22.010031101331336 27.352855388308445 27.92181716112531 22.715296349234908 80-81 22.04756425238214 27.484649572929754 27.801045501634785 22.66674067305332 82-83 21.989395977504543 27.374720287522265 27.95426107292622 22.68162266204698 84-85 21.867546200287357 27.377774913548496 27.809985870492053 22.944693015672094 86-87 21.907161447962213 27.368963769606864 27.903888515094643 22.819986267336276 88-89 21.905247976226402 27.248938230963038 27.703037311446078 23.142776481364482 90-91 21.828084458697365 27.19400430490639 27.870110467179714 23.107800769216535 92-93 21.96441882200955 27.353878052623752 27.789627905573315 22.89207521979338 94-95 21.785294806800493 27.160598453390687 27.75327040555376 23.30083633425506 96-97 21.563584956266485 27.135715456482544 27.92479995827808 23.375899628972896 98-99 21.580523108432146 27.239888349693587 27.566006929530896 23.61358161234337 100-101 21.571169326712226 26.63262240465586 28.31943195738861 23.476776311243306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 728.0 1 509.5 2 339.0 3 393.5 4 358.5 5 367.5 6 655.5 7 1444.5 8 2451.5 9 2960.0 10 2720.0 11 2407.5 12 2444.0 13 2676.5 14 2961.0 15 3143.0 16 3312.0 17 3510.5 18 3724.5 19 4078.5 20 4708.5 21 5466.0 22 6542.0 23 8153.0 24 9937.5 25 12500.0 26 16100.5 27 19906.5 28 23251.0 29 27255.5 30 32089.5 31 37222.5 32 42347.5 33 47363.0 34 53499.0 35 59324.0 36 65993.0 37 72671.0 38 76697.5 39 79138.0 40 81205.5 41 83907.5 42 87138.0 43 91303.0 44 95711.5 45 98951.0 46 99830.5 47 106370.0 48 123655.5 49 123289.0 50 107019.0 51 98045.5 52 91900.0 53 87855.0 54 83267.5 55 76765.0 56 70449.5 57 63232.0 58 55884.5 59 48686.0 60 40819.5 61 32272.0 62 24948.0 63 19689.0 64 15035.5 65 11183.5 66 7903.0 67 5798.0 68 4095.0 69 2530.0 70 1656.0 71 1079.5 72 720.0 73 488.0 74 325.0 75 209.0 76 125.0 77 57.5 78 26.0 79 16.0 80 9.0 81 4.5 82 3.0 83 1.5 84 0.5 85 0.5 86 1.5 87 1.0 88 0.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4098786605688384 2 0.0 3 0.0 4 0.0 5 3.725153690528387E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013410553285902193 16-17 0.0024586014357487356 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024027241303908094 36-37 0.00741305584415149 38-39 0.013205669832923133 40-41 0.0027007364256330805 42-43 6.891534327477517E-4 44-45 3.9114113750548065E-4 46-47 2.9801229524227096E-4 48-49 2.79386526789629E-4 50-51 5.58773053579258E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.607607583369871E-4 60-61 0.0024958529726540196 62-63 0.0 64-65 2.235092214317032E-4 66-67 0.0016949449291904161 68-69 0.002104711835148539 70-71 1.8625768452641935E-5 72-73 0.0 74-75 0.0 76-77 0.12607782665593326 78-79 7.450307381056774E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14835424572529302 88-89 0.44360992723657294 90-91 0.3402369123244102 92-93 0.0 94-95 0.0 96-97 4.8426997976869034E-4 98-99 0.0 100-101 2.4213498988434517E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2684453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.21659556834464 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68306810406386 39.211620355287614 2 14.472684475425746 15.403739930339839 3 4.997049394435385 7.97777869976128 4 2.3070666398318096 4.91096929284597 5 1.2934174012331334 3.4415635371241504 6 0.7629942381540025 2.4362373475691266 7 0.501630716454254 1.8686555273543501 8 0.3479732087752875 1.4814359616010901 9 0.259446175804624 1.2426157988593483 >10 1.241876001371375 11.726666167715763 >50 0.08787794756895954 3.2200330462516393 >100 0.04078554117001248 3.825117207208132 >500 0.0026601002887831084 0.9050621543922832 >1k 0.0011900448659978885 1.2090840618328083 >5k 2.8001055670538554E-4 1.1394209118566143 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9312 0.3468863116620034 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8074 0.300768908973262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8045 0.2996886144030087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5155 0.19203167274673835 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4031 0.15016094526519927 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2847 0.10605512556934318 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.725153690528387E-5 10-11 0.0 0.0 0.0 0.0 3.725153690528387E-5 12-13 0.0 0.0 0.0 0.0 3.725153690528387E-5 14-15 0.0 0.0 0.0 0.0 1.117546107158516E-4 16-17 0.0 0.0 0.0 3.725153690528387E-5 1.117546107158516E-4 18-19 0.0 0.0 0.0 1.117546107158516E-4 1.117546107158516E-4 20-21 0.0 0.0 0.0 2.235092214317032E-4 1.117546107158516E-4 22-23 0.0 0.0 0.0 3.5388960060019677E-4 1.3038037916849354E-4 24-25 0.0 0.0 0.0 5.40147285126616E-4 1.4900614762113548E-4 26-27 0.0 0.0 0.0 8.940368857268128E-4 1.4900614762113548E-4 28-29 0.0 0.0 0.0 0.0030546260262332772 1.4900614762113548E-4 30-31 0.0 0.0 0.0 0.008698233867383783 1.4900614762113548E-4 32-33 0.0 0.0 0.0 0.019687437254442525 1.8625768452641936E-4 34-35 0.0 0.0 0.0 0.0367300153886099 2.235092214317032E-4 36-37 0.0 0.0 0.0 0.06399814040327768 2.4213498988434514E-4 38-39 0.0 0.0 0.0 0.10259073263715177 3.166380636949129E-4 40-41 0.0 0.0 0.0 0.14567213506811258 3.3526383214755483E-4 42-43 0.0 0.0 0.0 0.18875353749907336 3.5388960060019677E-4 44-45 0.0 0.0 0.0 0.246251284712379 3.725153690528387E-4 46-47 0.0 0.0 0.0 0.31777423557052403 4.6564421131604835E-4 48-49 0.0 0.0 0.0 0.39497804580672485 4.842699797686903E-4 50-51 0.0 0.0 0.0 0.4785704946221819 4.842699797686903E-4 52-53 0.0 0.0 0.0 0.5606356304245222 5.40147285126616E-4 54-55 0.0 0.0 0.0 0.6610098966158096 5.960245904845419E-4 56-57 3.725153690528387E-5 0.0 0.0 0.781667624652024 5.960245904845419E-4 58-59 3.725153690528387E-5 0.0 0.0 0.9134635622229184 5.960245904845419E-4 60-61 3.725153690528387E-5 1.8625768452641935E-5 0.0 1.061780556411306 6.332761273898258E-4 62-63 3.725153690528387E-5 3.725153690528387E-5 0.0 1.2100044217574306 7.450307381056774E-4 64-65 3.725153690528387E-5 3.725153690528387E-5 0.0 1.36608836139057 7.450307381056774E-4 66-67 3.725153690528387E-5 3.725153690528387E-5 0.0 1.5527185612860421 7.450307381056774E-4 68-69 3.725153690528387E-5 3.725153690528387E-5 0.0 1.7679579415247724 7.450307381056774E-4 70-71 3.725153690528387E-5 3.725153690528387E-5 0.0 2.009813544882328 7.822822750109613E-4 72-73 3.725153690528387E-5 3.725153690528387E-5 0.0 2.255077663866717 8.56785348821529E-4 74-75 3.725153690528387E-5 3.725153690528387E-5 0.0 2.511703501607218 8.56785348821529E-4 76-77 3.725153690528387E-5 3.725153690528387E-5 0.0 2.7890970711724137 8.56785348821529E-4 78-79 3.725153690528387E-5 3.725153690528387E-5 0.0 3.114116730671016 8.56785348821529E-4 80-81 3.725153690528387E-5 3.725153690528387E-5 0.0 3.467112294385486 8.754111172741709E-4 82-83 3.725153690528387E-5 3.725153690528387E-5 0.0 3.833425282543595 9.312884226320967E-4 84-85 3.725153690528387E-5 3.725153690528387E-5 0.0 4.222815597814527 9.312884226320967E-4 86-87 3.725153690528387E-5 3.725153690528387E-5 0.0 4.631558086507754 9.499141910847386E-4 88-89 3.725153690528387E-5 3.725153690528387E-5 0.0 5.084741658728985 9.685399595373806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9055 0.0 22.4185 1 GAGTACT 8355 0.0 21.582748 12-13 TACATGG 9315 0.0 21.242548 2 ACATGGG 9285 0.0 20.442383 3 GTACTTT 9085 0.0 19.796293 14-15 AGTACTT 8995 0.0 19.3613 12-13 TTTGCGT 585 0.0 18.656296 3 GTATCAA 21740 0.0 18.258741 1 GGTATCA 17440 0.0 17.897 1 AGAGTAC 15900 0.0 17.145987 10-11 GTTCGCG 195 2.2944296E-4 17.034008 7 CATGGGG 4565 0.0 16.943388 4 CTTTGCG 660 0.0 16.53626 2 TACTTTT 10640 0.0 16.189543 14-15 ACTTTTT 11235 0.0 16.176449 16-17 CAACGCA 24460 0.0 15.985424 5 ATCAACG 24525 0.0 15.943058 3 TATCAAC 24750 0.0 15.932329 2 TCAACGC 24545 0.0 15.930067 4 AACGCAG 24875 0.0 15.718733 6 >>END_MODULE