##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938895_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1027279 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848963134649885 33.0 31.0 34.0 30.0 34.0 2 31.855898933006515 33.0 31.0 34.0 30.0 34.0 3 31.903763242507633 34.0 31.0 34.0 30.0 34.0 4 35.3832571287839 37.0 35.0 37.0 33.0 37.0 5 35.45944480516004 37.0 35.0 37.0 33.0 37.0 6 35.60911690008265 37.0 35.0 37.0 33.0 37.0 7 35.56960669886175 37.0 35.0 37.0 33.0 37.0 8 35.57998946732095 37.0 35.0 37.0 33.0 37.0 9 37.118042907525606 39.0 37.0 39.0 33.0 39.0 10-11 37.01914475035507 39.0 37.0 39.0 33.0 39.0 12-13 36.94194371733482 39.0 37.0 39.0 33.0 39.0 14-15 37.91797797871854 40.0 38.0 41.0 33.0 41.0 16-17 37.89307140513921 40.0 37.5 41.0 33.0 41.0 18-19 37.95233037957556 40.0 38.0 41.0 33.0 41.0 20-21 38.05772433778944 40.0 38.0 41.0 33.0 41.0 22-23 38.03978227920555 40.0 38.0 41.0 33.0 41.0 24-25 37.92197786579887 40.0 38.0 41.0 33.0 41.0 26-27 37.65744554303164 40.0 37.5 41.0 32.5 41.0 28-29 37.52071151069963 40.0 37.0 41.0 32.0 41.0 30-31 37.42317666378851 40.0 37.0 41.0 32.0 41.0 32-33 37.22902444224013 40.0 36.0 41.0 31.5 41.0 34-35 36.88941271066575 39.0 35.5 41.0 31.0 41.0 36-37 36.52011916918383 39.0 35.0 41.0 30.0 41.0 38-39 36.15792204454681 39.0 35.0 40.5 30.0 41.0 40-41 36.02362649290018 39.0 35.0 40.0 30.0 41.0 42-43 35.8926382219436 39.0 35.0 40.0 29.5 41.0 44-45 35.85728755284592 39.0 35.0 41.0 29.0 41.0 46-47 35.54340544292252 39.0 35.0 41.0 27.0 41.0 48-49 35.24675526317583 39.0 35.0 41.0 26.0 41.0 50-51 34.04919257572675 38.0 34.0 40.0 23.5 40.5 52-53 34.094342919498985 38.0 33.5 39.5 23.0 40.5 54-55 34.65351720418698 38.0 34.0 40.0 23.5 41.0 56-57 34.62497432537801 38.0 34.0 40.0 23.0 41.0 58-59 34.48907307557148 38.0 34.0 40.0 23.0 41.0 60-61 34.057291154593834 38.0 34.0 40.0 22.0 41.0 62-63 33.64541667842913 37.5 33.5 40.0 22.0 41.0 64-65 33.110257291349285 36.5 33.0 39.5 19.5 41.0 66-67 32.57708373285154 36.0 32.0 39.0 18.0 40.5 68-69 32.17704683927151 36.0 32.0 39.0 15.5 40.0 70-71 31.5252424122366 35.0 31.0 37.5 10.5 39.0 72-73 30.974613031123972 35.0 31.0 37.0 7.5 39.0 74-75 30.5371252600316 35.0 30.5 36.5 4.0 38.5 76-77 30.018085155055246 34.0 29.5 36.0 2.0 37.5 78-79 29.57914597689625 34.0 29.0 35.5 2.0 37.0 80-81 29.180108325002266 34.0 29.0 35.0 2.0 36.5 82-83 28.70378446361699 34.0 29.0 35.0 2.0 36.0 84-85 28.284486492958585 34.0 28.5 35.0 2.0 36.0 86-87 27.859953819751013 34.0 27.0 35.0 2.0 35.0 88-89 27.21329599845806 33.5 25.5 35.0 2.0 35.0 90-91 26.61063547488073 33.0 25.0 35.0 2.0 35.0 92-93 26.18483391561591 33.0 24.5 35.0 2.0 35.0 94-95 25.632977019874836 32.5 21.5 35.0 2.0 35.0 96-97 24.797333051683136 32.0 14.5 35.0 2.0 35.0 98-99 23.950335789984997 32.0 2.0 34.0 2.0 35.0 100-101 20.880585507929197 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.019220539038180107 1101 2 0.2120019816292178 1101 3 0.3010121988501808 1101 4 0.17235526334678752 1101 5 0.06990111689748346 1101 6 0.3659797650400378 1101 7 0.3304290194771582 1101 8 0.3100136473288089 1101 9 0.4516885046104875 1101 10-11 0.29611258272362306 1101 12-13 0.24664248996896987 1101 14-15 0.35782673572335 1101 16-17 0.24456914079402026 1101 18-19 0.588027923926937 1101 20-21 0.475350609205897 1101 22-23 0.46136158401204597 1101 24-25 0.6031681880894268 1101 26-27 0.6441131045911632 1101 28-29 0.3607969047477937 1101 30-31 0.35178490438664056 1101 32-33 0.5141921261570772 1101 34-35 0.5128473549774313 1101 36-37 0.49841734476256505 1101 38-39 0.6130436799409864 1101 40-41 0.4294366574587727 1101 42-43 0.3232347351677305 1101 44-45 0.47226788914598217 1101 46-47 0.19651376895136252 1101 48-49 0.2499015397831741 1101 50-51 0.29906699971851225 1101 52-53 0.4078169761032555 1101 54-55 0.3460859055537142 1101 56-57 0.4949923801329277 1101 58-59 0.27442553993218866 1101 60-61 0.30031463301320827 1101 62-63 0.4827446982714676 1101 64-65 0.3476835258497033 1101 66-67 0.3598527827884226 1101 68-69 0.192160758530747 1101 70-71 -0.03845967430174824 1101 72-73 0.13930222229268097 1101 74-75 -0.15392468692419214 1101 76-77 -0.048688310713316696 1101 78-79 -0.11989753145353532 1101 80-81 -0.25700223356430385 1101 82-83 -0.12952174824569695 1101 84-85 -0.13176303924452526 1101 86-87 -0.24501481759940447 1101 88-89 -0.41840690084836396 1101 90-91 -0.09491302334381757 1101 92-93 0.0842869912539399 1101 94-95 -0.14619315247041342 1101 96-97 -0.2850862039437949 1101 98-99 -0.5421006045808738 1101 100-101 -0.11255155120915461 1102 1 0.2357253934071153 1102 2 0.21250842573300233 1102 3 0.4257598284419615 1102 4 0.17629780578703702 1102 5 0.22077036120566618 1102 6 0.21513777332936712 1102 7 0.16689299494981924 1102 8 0.2931352052689675 1102 9 0.44865438208734787 1102 10-11 0.3915142746557194 1102 12-13 0.26347607879541357 1102 14-15 0.5097100037640061 1102 16-17 0.43839472416993175 1102 18-19 0.52293055266707 1102 20-21 0.3778385442538692 1102 22-23 0.47656966894930974 1102 24-25 0.4220792197137939 1102 26-27 0.4329934770433326 1102 28-29 0.43136239614619143 1102 30-31 0.5380575439142632 1102 32-33 0.3879751273493852 1102 34-35 0.5373622019654576 1102 36-37 0.4017489748085481 1102 38-39 0.3791306611480465 1102 40-41 0.2824747543190469 1102 42-43 0.28237741387096804 1102 44-45 0.2434885784095897 1102 46-47 0.2734279800454402 1102 48-49 0.2873806745132086 1102 50-51 0.2550722231330269 1102 52-53 0.20295636178428822 1102 54-55 0.4151621560505063 1102 56-57 0.43247662472498405 1102 58-59 0.32940027450324294 1102 60-61 0.3444729251837586 1102 62-63 0.49820316644838414 1102 64-65 0.2981706660302521 1102 66-67 0.15058462858414146 1102 68-69 0.3106351808324561 1102 70-71 -0.011598265115353001 1102 72-73 -0.1185453902662239 1102 74-75 -0.10336714420007098 1102 76-77 -0.10762262059806105 1102 78-79 0.024325485636115474 1102 80-81 -0.03410096745404445 1102 82-83 -0.10976645386086759 1102 84-85 -0.08777791462066986 1102 86-87 -0.41845489027300076 1102 88-89 -0.21901838100506765 1102 90-91 -0.09261869529067113 1102 92-93 -0.20084842008995452 1102 94-95 -0.1676312947516827 1102 96-97 -0.09000416227003427 1102 98-99 -0.13020541352880954 1102 100-101 0.16476690674803507 1103 1 0.2631517957513907 1103 2 0.23674956487304044 1103 3 0.3360927573893875 1103 4 0.35310714304603863 1103 5 0.28001926942165056 1103 6 0.3510925469949271 1103 7 0.19152461561034784 1103 8 0.15065811564245024 1103 9 0.5555767967694507 1103 10-11 0.45236392536594394 1103 12-13 0.5604233707423347 1103 14-15 0.5956785080112112 1103 16-17 0.5345261762397797 1103 18-19 0.6189409207045955 1103 20-21 0.6506889550705566 1103 22-23 0.6673981038831585 1103 24-25 0.7565086821817957 1103 26-27 0.7223622990057734 1103 28-29 0.7235144128036453 1103 30-31 0.7801844747410982 1103 32-33 0.7760396019895168 1103 34-35 0.7189268394027764 1103 36-37 0.9050338861159375 1103 38-39 0.8008310053974839 1103 40-41 0.9586740366209696 1103 42-43 0.8485408576167117 1103 44-45 1.0043516700267574 1103 46-47 1.1557296658364322 1103 48-49 1.0183655569689947 1103 50-51 0.9483043788806995 1103 52-53 0.8070973198196896 1103 54-55 0.9585241440069936 1103 56-57 1.0231019397462404 1103 58-59 1.150891705345721 1103 60-61 1.3401857393504812 1103 62-63 1.101960595156541 1103 64-65 0.8153634399205956 1103 66-67 1.0284027290827282 1103 68-69 1.001537772494224 1103 70-71 1.0008206694146793 1103 72-73 0.6942001814763579 1103 74-75 0.6075038845043785 1103 76-77 0.7237391866013958 1103 78-79 0.7328414588794381 1103 80-81 0.8842652207858563 1103 82-83 1.1096297018080108 1103 84-85 1.1204066707447318 1103 86-87 1.164115150177178 1103 88-89 1.0345576533943905 1103 90-91 1.0244962140353486 1103 92-93 0.9131484305665083 1103 94-95 0.8094137218582667 1103 96-97 1.3086409711367608 1103 98-99 0.9501335522397518 1103 100-101 1.0127653123783844 1104 1 0.1317597239911592 1104 2 0.2707777612035436 1104 3 0.23965708693246057 1104 4 0.29131473098139793 1104 5 0.17971027930192918 1104 6 0.10573778076901164 1104 7 0.04985045708371416 1104 8 0.11513491444645041 1104 9 0.22774086120730885 1104 10-11 0.20722241058480506 1104 12-13 0.16910248006961837 1104 14-15 0.28220225131483545 1104 16-17 0.24689823940260425 1104 18-19 0.2858797635573538 1104 20-21 0.24897542359296665 1104 22-23 0.3767363846500089 1104 24-25 0.3234937669268021 1104 26-27 0.20626181492499285 1104 28-29 0.35659902760981055 1104 30-31 0.38539431977464034 1104 32-33 0.33537344937107605 1104 34-35 0.4054657347827444 1104 36-37 0.43982305339149974 1104 38-39 0.4716308217767704 1104 40-41 0.26895488877374163 1104 42-43 0.1358113176898712 1104 44-45 0.27820805303532836 1104 46-47 0.3446625892780375 1104 48-49 0.42646316327764566 1104 50-51 0.4483778945103438 1104 52-53 0.3669210843362194 1104 54-55 0.47814552167866964 1104 56-57 0.5780265329129932 1104 58-59 0.5893939169876816 1104 60-61 0.5451675175757131 1104 62-63 0.5141240086162924 1104 64-65 0.41211898754469445 1104 66-67 0.18466496999624837 1104 68-69 0.4422729512382233 1104 70-71 0.46772797350114104 1104 72-73 0.39947546103216425 1104 74-75 0.4124708473660803 1104 76-77 0.4096018504262098 1104 78-79 0.4229281848749018 1104 80-81 0.37445902145411836 1104 82-83 0.3969562806824243 1104 84-85 0.3423671922904248 1104 86-87 0.49277322617775 1104 88-89 0.4443511698770415 1104 90-91 0.24646683696526495 1104 92-93 0.1285486642451943 1104 94-95 0.11002474336635615 1104 96-97 0.24781155770169505 1104 98-99 0.45936285029455703 1104 100-101 0.939739599866023 1105 1 0.13561398166113037 1105 2 0.3116007736913353 1105 3 0.3241916639062694 1105 4 0.28051531511555794 1105 5 0.4248881747059414 1105 6 0.3565621636595395 1105 7 0.41424696598276967 1105 8 0.44306800453329487 1105 9 0.32562508787192 1105 10-11 0.4656434282801314 1105 12-13 0.4066468040328246 1105 14-15 0.41027280992524595 1105 16-17 0.5947524885075666 1105 18-19 0.5168846970071783 1105 20-21 0.5861282192145225 1105 22-23 0.32733871947365145 1105 24-25 0.45367185504369445 1105 26-27 0.4111482642115263 1105 28-29 0.4046418141524484 1105 30-31 0.5831621692701603 1105 32-33 0.5040389768904774 1105 34-35 0.5470438605857169 1105 36-37 0.310779294719417 1105 38-39 0.48275463766316307 1105 40-41 0.7006445953362501 1105 42-43 0.2254640276612605 1105 44-45 0.6033280703366657 1105 46-47 0.512854364292167 1105 48-49 0.5183743611809319 1105 50-51 0.22831958815682185 1105 52-53 0.4269077871472575 1105 54-55 0.5809230496434523 1105 56-57 0.49939164674206893 1105 58-59 0.3317357211228753 1105 60-61 0.3768558323229527 1105 62-63 0.4371914195829447 1105 64-65 0.5979445267125172 1105 66-67 0.7803704704501939 1105 68-69 0.6515125704375677 1105 70-71 0.6232735008492369 1105 72-73 0.48087577708129103 1105 74-75 0.5321926556124765 1105 76-77 0.61519866081386 1105 78-79 0.6599018645775239 1105 80-81 0.6244408207928984 1105 82-83 0.8235412370864879 1105 84-85 0.9916720340945595 1105 86-87 0.9318941556533993 1105 88-89 1.163508543500214 1105 90-91 1.2150567782092487 1105 92-93 1.0947259472504882 1105 94-95 1.0679774687547834 1105 96-97 1.3447530971778612 1105 98-99 1.3806765740213685 1105 100-101 1.4288138499987824 1106 1 0.20280563470787172 1106 2 0.09154191908615061 1106 3 0.2380249724760901 1106 4 0.06708998534816857 1106 5 0.265233708194458 1106 6 0.22668787973766058 1106 7 0.25388054860209053 1106 8 0.24060568633872492 1106 9 0.2275956440603153 1106 10-11 0.24850511995794022 1106 12-13 0.2839250468142609 1106 14-15 0.18856718200522238 1106 16-17 0.2427485015827031 1106 18-19 0.3568814777510667 1106 20-21 0.3893104332372772 1106 22-23 0.5459664496504644 1106 24-25 0.395732983764411 1106 26-27 0.3137982691642307 1106 28-29 0.2968137595736735 1106 30-31 0.32172607150383215 1106 32-33 0.345429736800007 1106 34-35 0.47100286459740204 1106 36-37 0.5447108731454549 1106 38-39 0.6222825626462338 1106 40-41 0.7093984955888359 1106 42-43 0.5246933666406761 1106 44-45 0.5768202822132409 1106 46-47 0.6052279259450515 1106 48-49 0.6841825595531432 1106 50-51 0.46865304943494834 1106 52-53 0.5680231562060811 1106 54-55 0.6494070482897101 1106 56-57 0.5670594814208911 1106 58-59 0.5767221424657194 1106 60-61 0.8033273006339172 1106 62-63 0.723902805018696 1106 64-65 0.583776704415456 1106 66-67 0.7380496344341481 1106 68-69 0.671209361912311 1106 70-71 0.6054948494699133 1106 72-73 0.6873368859912041 1106 74-75 0.5079574352997973 1106 76-77 0.3384643897690047 1106 78-79 0.3270627696012163 1106 80-81 0.6073715407506342 1106 82-83 0.61254376296435 1106 84-85 0.5265498880889119 1106 86-87 0.6489163850295228 1106 88-89 0.5987643505429538 1106 90-91 0.7390134090513705 1106 92-93 0.9118531119938567 1106 94-95 0.8075721520295112 1106 96-97 0.7373243541735519 1106 98-99 1.1889053847126014 1106 100-101 1.2685206913307283 1107 1 0.07889795839302138 1107 2 0.16410936702819967 1107 3 0.055749040955447526 1107 4 0.2208081495946672 1107 5 0.05430052266998331 1107 6 0.1922806988261172 1107 7 0.1892172198167188 1107 8 0.1194958205036798 1107 9 -0.007895536136544479 1107 10-11 -1.6500641050498643E-4 1107 12-13 0.021103440732979095 1107 14-15 0.12926243181842523 1107 16-17 0.1496837418126944 1107 18-19 0.20805339081997687 1107 20-21 0.1236910166761831 1107 22-23 0.053178222382165075 1107 24-25 -0.01567306827730164 1107 26-27 0.019473885575884253 1107 28-29 0.15403862800754808 1107 30-31 0.3638668228925823 1107 32-33 0.1651227288735484 1107 34-35 0.2410880171166525 1107 36-37 0.12951772676595397 1107 38-39 0.35297046262350307 1107 40-41 0.21422867783228838 1107 42-43 0.3084310424682428 1107 44-45 -0.1363929767283807 1107 46-47 0.2838832511245357 1107 48-49 0.2809669048086434 1107 50-51 0.390138731467232 1107 52-53 0.4221070864597962 1107 54-55 0.39828985292214014 1107 56-57 0.19840176043852864 1107 58-59 0.38989773857395704 1107 60-61 0.16265864829333765 1107 62-63 0.1699221176263066 1107 64-65 0.2957565309430876 1107 66-67 0.19661848737246146 1107 68-69 0.42012871269033525 1107 70-71 0.2060256822347526 1107 72-73 0.4698614328189983 1107 74-75 0.40630027063098595 1107 76-77 0.4739952631406723 1107 78-79 0.6723456773749881 1107 80-81 0.542242248608872 1107 82-83 0.5277734554894451 1107 84-85 0.744200458208784 1107 86-87 0.7755284761187617 1107 88-89 0.7630012061125875 1107 90-91 0.6270659664694271 1107 92-93 0.6645798605238902 1107 94-95 0.6735680954073509 1107 96-97 0.47640467041953016 1107 98-99 0.23249935297430468 1107 100-101 0.18458130108286497 1108 1 0.15021227457537378 1108 2 0.18727818867575863 1108 3 0.08467466079015651 1108 4 0.1582165073659283 1108 5 0.1431851534351125 1108 6 0.22018159192128905 1108 7 0.2377126210430589 1108 8 0.26692317320962644 1108 9 0.29765631670269954 1108 10-11 0.3461093204356729 1108 12-13 0.3226896844013325 1108 14-15 0.374251268128738 1108 16-17 0.2634034165660921 1108 18-19 0.3393850357146633 1108 20-21 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2316 80-81 -2.345578921707556 2316 82-83 -2.293721396810671 2316 84-85 -2.16103506668993 2316 86-87 -2.12356891349096 2316 88-89 -2.5642599675373425 2316 90-91 -2.5135750599934674 2316 92-93 -2.60508460944893 2316 94-95 -2.849036429183659 2316 96-97 -2.9708899023790742 2316 98-99 -3.042400161103341 2316 100-101 -2.5220302559200576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3428.0 3 782.0 4 746.0 5 786.0 6 890.0 7 1166.0 8 1398.0 9 1892.0 10 2375.0 11 3300.0 12 4664.0 13 7054.0 14 6107.0 15 6773.0 16 8609.0 17 11553.0 18 9696.0 19 6951.0 20 7027.0 21 7406.0 22 8321.0 23 9268.0 24 10382.0 25 11895.0 26 13889.0 27 16420.0 28 19412.0 29 23669.0 30 27403.0 31 33257.0 32 40917.0 33 52618.0 34 70993.0 35 104154.0 36 145425.0 37 194316.0 38 136827.0 39 15507.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.386899704893764 17.05434759256981 14.1385378276978 18.420214874838628 2 13.64519416444693 24.020116952683072 37.94902172451622 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26.730822992634963 23.894878955627615 22-23 19.7935714793703 26.914766035011695 27.159929738897976 26.131732746720026 24-25 23.451809192178168 26.764411158207434 28.229117089275107 21.55466256033929 26-27 24.672701800602866 22.780714590514346 32.479876933149136 20.066706675733656 28-29 20.089716942024967 27.330689594870865 32.01747186055246 20.562121602551706 30-31 19.915488910050488 28.85583674503223 29.834850230274455 21.393824114642825 32-33 20.164947188260722 28.630021693579888 29.157798145476544 22.047232972682846 34-35 22.891999808605526 25.373585414178617 28.661810845621805 23.072603931594053 36-37 23.470092459629612 24.616349272804815 31.74846715785437 20.165091109711195 38-39 24.77984241557733 23.94874447833532 31.719806016841996 19.55160708924536 40-41 21.002015607637507 26.315143651764792 32.82688115639072 19.855959584206985 42-43 19.3047591196328 29.037045803311106 28.93454560036462 22.723649476691474 44-45 20.570046026829665 28.131533733689967 27.69043716929373 23.607983070186638 46-47 21.96606844018697 27.996764510553973 26.07581491200701 23.961352137252046 48-49 22.703160638006995 26.48485224969906 27.714535452197165 23.09745166009678 50-51 22.387047168879242 27.92955433190872 29.759220474574548 19.924178024637495 52-53 21.212668125190447 28.88141203883723 29.05377413815282 20.852145697819502 54-55 18.30259132735529 26.458926837856804 31.229052601720053 24.00942923306785 56-57 22.17244085440539 28.541169049929955 26.759259032889766 22.527131062774885 58-59 22.832583154038282 26.623714074380434 27.272158399539283 23.271544372042 60-61 21.214696374773684 25.498263986453964 30.844452697601344 22.442586941171008 62-63 24.356481639958336 25.337444313497283 28.937860953675674 21.368213092868704 64-65 22.44194589475764 27.889694020600807 29.432602258086295 20.235757826555258 66-67 20.704339590520487 26.343488461741664 29.4153185960367 23.536853351701144 68-69 19.547450033734048 29.19489864863212 29.21715969586028 22.040491621773555 70-71 22.22964239341128 28.479318270548898 25.962706056523118 23.32833327951671 72-73 20.839109731841397 26.03473763818107 28.108468779075622 25.0176838509019 74-75 20.43634865822225 29.980662276665925 27.377334114082295 22.205654951029526 76-77 23.067815302152592 25.281098621099453 27.91531462358387 23.735771453164087 78-79 21.159761716509536 25.870276026867767 30.43366011010563 22.536302146517063 80-81 21.800845665341654 28.89584780957811 28.25683111680734 21.046475408272887 82-83 20.91700128958808 27.466416671434768 28.597361454862707 23.019220584114446 84-85 21.101134585289515 26.526066118935837 28.11913145539906 24.253667840375588 86-87 22.28221692969029 27.351881103867843 28.375350277042855 21.990551689399016 88-89 23.32614394428468 25.38768902713383 27.908817027603337 23.377350000978147 90-91 21.413799310504054 24.868175966253048 29.95981201256902 23.75821271067388 92-93 21.593032561543293 28.48415948718651 28.672296761847367 21.25051118942283 94-95 21.145750728795225 28.541083797630968 27.514386337052443 22.79877913652136 96-97 19.304544682999225 27.00962333824618 27.958602965023253 25.727229013731346 98-99 21.85698575884349 27.534565818876285 26.29668293370172 24.31176548857851 100-101 23.223805995515225 26.0438353886521 27.67542347391665 23.056935141916032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6073.0 1 3720.0 2 1433.5 3 1427.0 4 1185.5 5 1188.0 6 1841.0 7 3306.0 8 4855.0 9 5022.5 10 3664.0 11 2564.5 12 2418.0 13 2406.0 14 2437.5 15 2592.5 16 2774.5 17 2648.5 18 2428.5 19 2386.5 20 2397.0 21 2561.5 22 2866.5 23 3113.0 24 3460.0 25 3917.5 26 4450.5 27 5069.5 28 5587.0 29 6283.0 30 7371.0 31 8381.5 32 9434.0 33 10413.0 34 11684.0 35 12732.0 36 13494.5 37 14931.5 38 16012.5 39 16125.0 40 16020.5 41 16612.5 42 17901.5 43 20468.5 44 25776.0 45 30069.0 46 37157.0 47 65989.0 48 166412.5 49 164382.0 50 61580.0 51 31182.5 52 21168.0 53 18239.5 54 16562.0 55 14711.5 56 13165.0 57 11527.0 58 10601.0 59 9925.5 60 9193.0 61 8390.5 62 7273.5 63 5386.0 64 3602.5 65 2728.5 66 1959.5 67 1457.0 68 1043.0 69 686.0 70 534.0 71 437.5 72 367.5 73 316.5 74 240.5 75 188.5 76 154.0 77 84.0 78 47.0 79 31.0 80 21.5 81 17.0 82 14.0 83 9.5 84 5.5 85 4.5 86 3.0 87 1.5 88 2.0 89 1.0 90 0.0 91 1.5 92 2.0 93 1.5 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3154936487555961 2 0.0857605382763592 3 0.09530030303354785 4 0.09987549633546486 5 0.11184887455112 6 0.05607045408306799 7 0.057335933081470565 8 0.05860141207987314 9 0.061813781845048904 10-11 0.05845539527236515 12-13 0.05714124400479324 14-15 0.05519435323802005 16-17 0.056897882658946596 18-19 0.06453942891853139 20-21 0.06541552976357932 22-23 0.08001721051437827 24-25 0.08717203408226976 26-27 0.08225613489616745 28-29 0.08064995001357957 30-31 0.07656147940335586 32-33 0.06891993314377107 34-35 0.3130600352971296 36-37 0.4862359690016052 38-39 0.4590768428051191 40-41 0.4390725401765246 42-43 0.47134225463579027 44-45 0.4809306916621483 46-47 0.4738732126325954 48-49 0.49300141441614204 50-51 0.4882802043067171 52-53 0.4879394984225317 54-55 0.47956786812540697 56-57 0.4571786243075153 58-59 0.4403380191749272 60-61 0.39925862399601275 62-63 0.46861660756230783 64-65 0.4950943219904232 66-67 0.5062889438993692 68-69 0.517337549000807 70-71 0.5149526078115099 72-73 0.5022978178274841 74-75 0.5045854144784425 76-77 0.47762097735863385 78-79 0.4590281705359498 80-81 0.46438212014457614 82-83 0.4730457840567168 84-85 0.4749440025543207 86-87 0.47445727986262737 88-89 0.48078467485464027 90-91 0.49480228837540724 92-93 0.5016164060591135 94-95 0.5077004397052797 96-97 0.49923146486981634 98-99 0.5033686077492093 100-101 0.48005459081710034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1027279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.308094420200145 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86462296990894 22.812524958341932 2 13.38498550651955 8.38116780102249 3 5.263574185010346 4.943774327160958 4 2.6815890210104296 3.3582176906386643 5 1.5737078530960993 2.463489702727157 6 0.9546463021691739 1.793289393972445 7 0.6525720539756118 1.430155123729668 8 0.4371649623638115 1.0949441535111564 9 0.31961696316839067 0.9005938255056245 >10 1.6543428536694615 9.220845570956564 >50 0.10596536164266253 2.261316470836238 >100 0.07666902324573599 4.660310675723613 >500 0.00841489279526002 1.7055248068707514 >1k 0.014959809413795589 11.297308892928406 >5k 0.004363277745690381 8.912755129537626 >10k+ 0.002804964265086673 14.763781476536753 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 29767 2.8976548727268834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24217 2.357392684947322 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 23742 2.3111540292364587 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17291 1.683184412413765 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14698 1.4307700245016204 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10622 1.0339936862332433 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10553 1.0272769130878758 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10364 1.0088787953418692 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10052 0.9785072993802072 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8275 0.805526054752409 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8193 0.797543802608639 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7788 0.7581192645814818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7564 0.736314087993622 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6945 0.6760578187619917 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6376 0.6206687764472942 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6258 0.6091821209233325 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6038 0.5877663224888273 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5999 0.5839698854936195 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5771 0.5617753307524052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5764 0.5610939189840345 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5634 0.5484391290000087 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5568 0.5420143894696573 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5169 0.5031739186725319 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4964 0.48321828831310676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4870 0.47406790170927277 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4825 0.4696873974840331 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4629 0.4506078679696558 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4588 0.4466167418977707 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4292 0.4178027585495275 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4124 0.4014488761086326 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4037 0.3929799012731692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3973 0.38674985081949503 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3563 0.34683859010064455 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3539 0.3445023211805167 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 3432 0.3340864555782801 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3277 0.31899805213578786 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3250 0.316369749600644 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3060 0.29787428731629867 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2997 0.2917415814009631 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2977 0.28979469063418994 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2896 0.28190978302875846 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2687 0.26156477451597865 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2570 0.2501754635303554 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2288 0.22272430371885338 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2270 0.22097210202875756 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2138 0.20812262296805445 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 2123 0.20666245489297455 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2050 0.1995563035942524 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2022 0.19683065652076992 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1890 0.18398117746006684 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1716 0.16704322778914005 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1682 0.16373351348562562 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1605 0.15623798403354883 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1454 0.1415389587444112 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1423 0.13852127805591274 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1318 0.12830010153035348 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1309 0.12742400068530554 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1279 0.12450366453514575 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1258 0.12245942923003389 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1246 0.12129129476997 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 1244 0.12109660569329267 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1206 0.1173975132364236 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 0.11622937877635968 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.11584000062300505 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1144 0.11136215185942669 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1065 0.1036719333306726 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 1047 0.10191973164057672 No Hit GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA 1034 0.10065425264217413 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.867226916932985E-5 0.0 14-15 0.0 0.0 0.0 9.73445383386597E-5 0.0 16-17 0.0 0.0 0.0 2.4336134584664924E-4 0.0 18-19 0.0 0.0 0.0 4.867226916932985E-4 0.0 20-21 0.0 0.0 0.0 8.761008450479373E-4 0.0 22-23 0.0 0.0 0.0 0.002871663880990461 0.0 24-25 0.0 0.0 0.0 0.005256605070287624 0.0 26-27 0.0 0.0 0.0 0.008809680719648704 0.0 28-29 0.0 0.0 0.0 0.023314016932109 0.0 30-31 0.0 0.0 0.0 0.05626514315974531 0.0 32-33 0.0 0.0 0.0 0.11170285774361202 0.0 34-35 0.0 0.0 0.0 0.20807395069888512 0.0 36-37 0.0 0.0 0.0 0.33895368249521307 0.0 38-39 0.0 0.0 0.0 0.5109614817396247 0.0 40-41 0.0 0.0 0.0 0.6902214490902666 0.0 42-43 0.0 0.0 0.0 0.8698221223250937 0.0 44-45 0.0 0.0 0.0 1.068453652805129 0.0 46-47 0.0 0.0 0.0 1.3327927466637592 0.0 48-49 0.0 0.0 0.0 1.6094459246222304 0.0 50-51 0.0 0.0 0.0 1.89271853118773 0.0 52-53 0.0 0.0 0.0 2.1421639106805452 0.0 54-55 0.0 0.0 0.0 2.4002729540855015 0.0 56-57 0.0 0.0 0.0 2.7159126196486056 0.0 58-59 0.0 0.0 0.0 3.092830672095896 0.0 60-61 0.0 0.0 0.0 3.616787649703732 0.0 62-63 0.0 0.0 0.0 4.087837870724506 0.0 64-65 0.0 0.0 0.0 4.486074377067963 0.0 66-67 0.0 0.0 0.0 4.944956530796405 0.0 68-69 0.0 0.0 0.0 5.456842785650247 0.0 70-71 0.0 0.0 0.0 6.0256755954322045 0.0 72-73 0.0 0.0 0.0 6.641574489500904 0.0 74-75 0.0 0.0 0.0 7.301229753552832 0.0 76-77 0.0 0.0 0.0 7.843779537983352 0.0 78-79 0.0 0.0 0.0 8.557022970390712 0.0 80-81 0.0 0.0 0.0 9.3219563526559 0.0 82-83 0.0 0.0 0.0 10.035297129601599 0.0 84-85 0.0 0.0 0.0 11.022468092893947 0.0 86-87 0.0 0.0 0.0 12.067023661536934 0.0 88-89 0.0 0.0 0.0 12.933195363674328 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 185 0.0 38.554398 94-95 AGTTCGC 30 0.004007068 31.57673 10-11 TACCTGG 405 0.0 24.572847 2 GAGTACT 4650 0.0 23.990469 12-13 CTTTGCG 1150 0.0 23.901293 2 TTTGCGT 1165 0.0 23.59355 3 CCACGAG 125 2.3002885E-4 22.734138 8 AGTACTT 4655 0.0 22.285645 12-13 GTACTTT 5145 0.0 21.774946 14-15 GCTTTGC 1280 0.0 21.510515 1 TTGCGTT 1300 0.0 21.143452 4 ACAGTAC 160 5.078083E-5 20.733341 3 GTCGGGA 375 0.0 20.219944 2 ATGGGAT 1150 0.0 20.192472 5 CGGGAGT 380 0.0 19.953894 4 ATGGGAC 500 0.0 19.904007 5 CGTCACT 120 0.0047797253 19.779785 1 ACTTTTT 5780 0.0 19.631456 16-17 CATGGGG 1315 0.0 19.460735 4 CCGTATC 75 7.668951E-4 19.020172 94-95 >>END_MODULE