##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938895_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1027279 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.072738759382794 33.0 31.0 34.0 30.0 34.0 2 32.282188188408405 34.0 31.0 34.0 30.0 34.0 3 32.37123702518985 34.0 31.0 34.0 30.0 34.0 4 35.86302942043982 37.0 35.0 37.0 35.0 37.0 5 35.810139212424275 37.0 35.0 37.0 35.0 37.0 6 35.911438859355634 37.0 35.0 37.0 35.0 37.0 7 35.870073271234006 37.0 35.0 37.0 35.0 37.0 8 35.87934339162 37.0 35.0 37.0 35.0 37.0 9 37.47316162405734 39.0 37.0 39.0 34.0 39.0 10-11 37.38233478928315 39.0 37.0 39.0 34.0 39.0 12-13 37.300780995231094 39.0 37.0 39.0 34.0 39.0 14-15 38.3343254364199 40.0 37.5 41.0 33.5 41.0 16-17 38.497789792257024 40.0 38.0 41.0 34.0 41.0 18-19 38.68998100808057 40.0 38.0 41.0 34.0 41.0 20-21 38.66731919955533 40.0 38.0 41.0 34.0 41.0 22-23 38.63400497819969 40.0 38.0 41.0 34.0 41.0 24-25 38.497634527718375 40.0 38.0 41.0 34.0 41.0 26-27 38.1484830313868 40.0 37.5 41.0 33.5 41.0 28-29 37.95615894026842 40.0 37.0 41.0 33.0 41.0 30-31 37.86263128127801 40.0 37.0 41.0 33.0 41.0 32-33 37.77707370636409 40.0 37.0 41.0 33.0 41.0 34-35 37.64755339104566 40.0 36.5 41.0 33.0 41.0 36-37 37.41271650642133 40.0 36.0 41.0 32.5 41.0 38-39 37.154279898644866 39.5 35.5 41.0 31.5 41.0 40-41 36.977302173995575 39.0 35.0 41.0 31.0 41.0 42-43 36.86291504060728 39.0 35.0 41.0 31.0 41.0 44-45 36.66732017300072 39.0 35.0 41.0 30.5 41.0 46-47 36.29878981269937 39.0 35.0 41.0 30.0 41.0 48-49 35.91070682842734 39.0 35.0 41.0 28.5 41.0 50-51 35.63720761351104 39.0 35.0 40.0 28.0 41.0 52-53 35.25050010756571 38.0 35.0 40.0 27.0 41.0 54-55 35.032750109755966 38.0 34.0 40.0 27.0 41.0 56-57 34.9716464563181 38.0 34.0 40.0 26.5 41.0 58-59 34.778560157464526 38.0 34.0 40.0 26.0 41.0 60-61 34.31173420268496 38.0 34.0 40.0 25.5 41.0 62-63 33.808509178129796 37.0 33.0 39.5 24.0 41.0 64-65 33.24027893104015 36.0 32.0 39.0 22.0 40.5 66-67 32.72018215110014 36.0 32.0 39.0 22.0 40.0 68-69 32.243016259458244 35.0 31.5 38.0 20.5 39.5 70-71 31.56943683264235 35.0 31.0 37.0 18.5 39.0 72-73 30.972421318843274 34.5 30.5 36.5 16.0 39.0 74-75 30.46185700282007 34.0 30.0 36.0 12.0 37.5 76-77 28.470010581351318 31.5 27.0 34.0 7.5 36.0 78-79 29.3879038703215 34.0 29.0 35.0 7.0 36.0 80-81 29.444736045417066 34.0 29.0 35.0 4.0 36.0 82-83 29.049180407659456 34.0 29.0 35.0 2.0 36.0 84-85 28.649758244839035 34.0 29.0 35.0 2.0 35.5 86-87 28.009495959714936 33.0 27.5 35.0 2.0 35.0 88-89 27.182534150897663 32.5 25.5 35.0 2.0 35.0 90-91 26.47727248391138 32.0 25.0 34.0 2.0 35.0 92-93 26.174518314888168 32.0 24.5 34.0 2.0 35.0 94-95 25.62894598254223 32.0 23.5 34.0 2.0 35.0 96-97 24.63390958055212 31.0 18.5 34.0 2.0 35.0 98-99 23.43378721846743 31.0 2.0 34.0 2.0 35.0 100-101 18.032876170933115 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14314034900868933 1101 2 0.21151028127790283 1101 3 0.08505343162646284 1101 4 0.06788767977302967 1101 5 0.0824587085490478 1101 6 0.030927612313845998 1101 7 0.062228406488614496 1101 8 0.14963601859578546 1101 9 0.24302794962112984 1101 10-11 0.18403167099467765 1101 12-13 0.12126475554592986 1101 14-15 0.23015988750384508 1101 16-17 0.17721866045017975 1101 18-19 0.21073789471350324 1101 20-21 0.3271451333246844 1101 22-23 0.1051797334150919 1101 24-25 0.15886332362799038 1101 26-27 0.055721312134288326 1101 28-29 0.14665155896161508 1101 30-31 0.056776830492687225 1101 32-33 0.24880111890465884 1101 34-35 0.35944801887302447 1101 36-37 0.349341286043547 1101 38-39 0.5490564661086736 1101 40-41 0.405742005440203 1101 42-43 0.3796057790384495 1101 44-45 0.1653242730074993 1101 46-47 0.32835622098728834 1101 48-49 0.24785972971053383 1101 50-51 0.2817327490921002 1101 52-53 0.27178048932306353 1101 54-55 0.18829593729176253 1101 56-57 0.292293483288141 1101 58-59 0.29130601120023414 1101 60-61 0.2586436441802107 1101 62-63 0.24740720592004095 1101 64-65 0.20408585145806057 1101 66-67 0.10306230367672953 1101 68-69 0.32613170783945833 1101 70-71 0.35303496515620836 1101 72-73 0.4244987647785301 1101 74-75 0.3302939848427364 1101 76-77 0.2710542535266889 1101 78-79 0.5291800160436999 1101 80-81 0.5265659552371567 1101 82-83 0.43789301740201836 1101 84-85 0.4730672616408782 1101 86-87 0.7105990572271637 1101 88-89 0.8537166236630256 1101 90-91 0.8217289907523977 1101 92-93 0.9260069411137586 1101 94-95 0.8869057370062166 1101 96-97 0.7120266318958492 1101 98-99 0.7832482318848086 1101 100-101 1.1158996793598845 1102 1 0.06284738358542086 1102 2 0.19618183070161876 1102 3 0.149821500666377 1102 4 0.08815679917347552 1102 5 0.0293286341721668 1102 6 0.04280179615533086 1102 7 0.0481865056199382 1102 8 0.1055821947156943 1102 9 0.21200086735235146 1102 10-11 0.21508657362341665 1102 12-13 0.1798385362760726 1102 14-15 0.25341543738178274 1102 16-17 0.17945791554584645 1102 18-19 0.029428634507972617 1102 20-21 0.19140164839877372 1102 22-23 0.14505482567648897 1102 24-25 0.0482558178084318 1102 26-27 0.13670127011986466 1102 28-29 0.2115223930046497 1102 30-31 0.0766110438578167 1102 32-33 0.052365531554407596 1102 34-35 0.12236347904449474 1102 36-37 0.11822449730794915 1102 38-39 0.0750463599370974 1102 40-41 0.01607755304709002 1102 42-43 0.019421824005235067 1102 44-45 0.09937071597575908 1102 46-47 0.1788524453670064 1102 48-49 0.10006151248462203 1102 50-51 -0.10760921423044323 1102 52-53 -0.2369437484558503 1102 54-55 -0.22453379075003 1102 56-57 -0.21235535563765495 1102 58-59 -0.37300500906888345 1102 60-61 -0.3210508996226622 1102 62-63 -0.08059540578722135 1102 64-65 -0.10387077149088952 1102 66-67 -0.31293610658841686 1102 68-69 -0.33986670242568806 1102 70-71 -0.12120962690647374 1102 72-73 0.013169065124866819 1102 74-75 -0.2874133903914675 1102 76-77 -0.38685921947620727 1102 78-79 0.035674650688566345 1102 80-81 0.03499722759280033 1102 82-83 0.07688807786871621 1102 84-85 0.4503306466405945 1102 86-87 0.5370698458492065 1102 88-89 0.4436337303455886 1102 90-91 0.35709150707556603 1102 92-93 0.5016794557336404 1102 94-95 0.3709658062732366 1102 96-97 0.5864165711452678 1102 98-99 0.7729927387831204 1102 100-101 0.583315792458162 1103 1 0.08470855523854937 1103 2 0.13711715560658178 1103 3 0.13781390423656603 1103 4 0.039960719515242715 1103 5 0.21472508878535734 1103 6 0.12349474442984842 1103 7 0.06347437856165072 1103 8 0.18718703900394473 1103 9 0.13694846519577908 1103 10-11 0.06468688044687809 1103 12-13 0.1834774300894324 1103 14-15 0.07033174572081435 1103 16-17 0.1936311201709131 1103 18-19 0.11911598279084501 1103 20-21 0.2090463148499211 1103 22-23 0.26075438432808795 1103 24-25 0.14671509591585874 1103 26-27 0.06012517894416902 1103 28-29 0.23858496390253947 1103 30-31 0.27168553081492774 1103 32-33 0.17808146262109403 1103 34-35 0.05226219717592073 1103 36-37 0.18401368131744533 1103 38-39 0.18291253485195824 1103 40-41 0.09863137171302583 1103 42-43 0.05625454380751904 1103 44-45 0.14364865539202754 1103 46-47 0.259322923457745 1103 48-49 0.338847914458114 1103 50-51 0.3985318898010135 1103 52-53 0.23158714882897158 1103 54-55 0.17939153342494762 1103 56-57 0.42595621153733987 1103 58-59 0.2500922625428714 1103 60-61 0.29363827361092376 1103 62-63 0.22262739926053143 1103 64-65 0.375428493778152 1103 66-67 0.2628259986284007 1103 68-69 0.39325308270520054 1103 70-71 0.3909619254139933 1103 72-73 0.3479681525336318 1103 74-75 0.2651382383336056 1103 76-77 0.21390065524526847 1103 78-79 0.3706730343036355 1103 80-81 0.4878978564186802 1103 82-83 0.586593339636174 1103 84-85 0.7931317084722416 1103 86-87 0.793101742511805 1103 88-89 0.7460581918692526 1103 90-91 0.8110737813001911 1103 92-93 0.8549435683962479 1103 94-95 0.9129529980158857 1103 96-97 0.7037666963426794 1103 98-99 1.1099829257623597 1103 100-101 1.6257815268357163 1104 1 0.01806747874525172 1104 2 0.032212035663867766 1104 3 -0.01877101978418949 1104 4 -0.04875973814480261 1104 5 -0.07878971030372384 1104 6 0.00929971812696806 1104 7 0.04374300803951314 1104 8 0.04477323467673955 1104 9 -0.023687113899740098 1104 10-11 0.012980139344236363 1104 12-13 0.003933182098201371 1104 14-15 0.0920968616005311 1104 16-17 0.14598289077520832 1104 18-19 0.20340137158750338 1104 20-21 0.02024583177928463 1104 22-23 0.024231595044092558 1104 24-25 -0.03691460080691655 1104 26-27 -0.16757517359413754 1104 28-29 -0.05967467983285957 1104 30-31 -0.07923262889244853 1104 32-33 0.12139530028287737 1104 34-35 0.06961300814872118 1104 36-37 0.22389114030302437 1104 38-39 0.13087244806984444 1104 40-41 0.2051392446196587 1104 42-43 0.10164174670054393 1104 44-45 0.30722934917754685 1104 46-47 0.3140511008200946 1104 48-49 0.2103813158074388 1104 50-51 0.28045793195521895 1104 52-53 0.21108863429363822 1104 54-55 0.44386597523929083 1104 56-57 0.31665249995205613 1104 58-59 0.19990690214232387 1104 60-61 -0.01089713566910433 1104 62-63 0.009272134699671142 1104 64-65 0.12205098372446344 1104 66-67 0.13665522013715758 1104 68-69 0.2549701833385498 1104 70-71 0.23483829793957156 1104 72-73 0.40369636693438693 1104 74-75 0.30918195712453667 1104 76-77 0.15369023218807243 1104 78-79 0.3602579459782511 1104 80-81 0.26632287103762664 1104 82-83 0.24383373125562002 1104 84-85 0.1839862519065143 1104 86-87 0.22854604067324402 1104 88-89 0.3991497205117298 1104 90-91 0.25540000213665337 1104 92-93 0.617318605855548 1104 94-95 0.6174919988991938 1104 96-97 0.7030803634032985 1104 98-99 0.8806562503636073 1104 100-101 0.8798330236925018 1105 1 -0.0026534387393724046 1105 2 0.08822296462561496 1105 3 0.05587310375761234 1105 4 0.04367499642531669 1105 5 0.014369838833779625 1105 6 -0.104193181473903 1105 7 0.05111106395194298 1105 8 0.11960150608499731 1105 9 0.14724279000076734 1105 10-11 0.18957847858743548 1105 12-13 0.18201648117146618 1105 14-15 0.006464891817905993 1105 16-17 -0.015581166987267636 1105 18-19 0.1746507506237691 1105 20-21 0.18651743703477308 1105 22-23 0.17709841331155474 1105 24-25 0.12909951862368274 1105 26-27 0.13738654077966572 1105 28-29 0.11125790063547214 1105 30-31 0.22507493230460085 1105 32-33 0.35578127421096184 1105 34-35 0.2560852061033927 1105 36-37 0.4117021574844415 1105 38-39 0.20753791563412705 1105 40-41 0.4077329459061261 1105 42-43 0.43803653831368905 1105 44-45 0.35536849216193644 1105 46-47 0.22802835213482808 1105 48-49 0.11868263738956841 1105 50-51 0.13423274909209937 1105 52-53 0.23216767655343062 1105 54-55 0.42791737818908615 1105 56-57 0.41203895352110465 1105 58-59 0.44796692578177755 1105 60-61 0.18074351475829076 1105 62-63 0.08570099366809814 1105 64-65 -0.035696288317609515 1105 66-67 0.06352175147655714 1105 68-69 0.10216492613108841 1105 70-71 0.5636658969939994 1105 72-73 0.6733123972202897 1105 74-75 0.3574003265165935 1105 76-77 0.1818049006362621 1105 78-79 0.32724515840780555 1105 80-81 0.35233170157020055 1105 82-83 0.3603779181785498 1105 84-85 0.3863556568091262 1105 86-87 0.7796208432495995 1105 88-89 0.7612058385034075 1105 90-91 0.7924699312183137 1105 92-93 1.0538197107427472 1105 94-95 0.962644520440211 1105 96-97 0.8217893583841978 1105 98-99 0.98819646311863 1105 100-101 0.969776944243403 1106 1 0.14574807678734913 1106 2 0.0533600719176448 1106 3 -0.0666484817672881 1106 4 0.06311744603157621 1106 5 0.10381769513939076 1106 6 0.20701602064585245 1106 7 0.08126269916895268 1106 8 0.12831413382343015 1106 9 0.16727027749519152 1106 10-11 0.17468890319906194 1106 12-13 0.2419219877503096 1106 14-15 0.04954823753299564 1106 16-17 0.03063785799086105 1106 18-19 0.06516096144376604 1106 20-21 0.09124912442024424 1106 22-23 0.13077362751590016 1106 24-25 0.25015128673818765 1106 26-27 0.1927701275262379 1106 28-29 0.20217866979004384 1106 30-31 0.3020760301534793 1106 32-33 0.31262260615753945 1106 34-35 0.24939183612006843 1106 36-37 0.2560598952553974 1106 38-39 0.22281700037485308 1106 40-41 0.4623519593279468 1106 42-43 0.24329475979850912 1106 44-45 0.38076604965455374 1106 46-47 0.5168657879560641 1106 48-49 0.5173498447261693 1106 50-51 0.4743007249229123 1106 52-53 0.25458432671109676 1106 54-55 0.4563814834835256 1106 56-57 0.5159156807758194 1106 58-59 0.6231770028788262 1106 60-61 0.6873053286027471 1106 62-63 0.5787269705885052 1106 64-65 0.7307270768781109 1106 66-67 0.6302356751719316 1106 68-69 0.6964571437858211 1106 70-71 0.5743828212011515 1106 72-73 0.6380594792535383 1106 74-75 0.6534444592159794 1106 76-77 0.48682941650967493 1106 78-79 0.6656622336723608 1106 80-81 0.4914252333417828 1106 82-83 0.6328673111424123 1106 84-85 0.7856161565367294 1106 86-87 0.886550188832615 1106 88-89 0.9984608861318875 1106 90-91 1.3028102437337914 1106 92-93 1.4917768038370305 1106 94-95 1.5318280882014257 1106 96-97 1.3718384726760462 1106 98-99 1.343662182168373 1106 100-101 1.7082779058929844 1107 1 -0.011263301245982404 1107 2 -0.003742265411403878 1107 3 0.024408270336145677 1107 4 -0.014561386440888668 1107 5 -0.03738849009271661 1107 6 -0.08619675101382995 1107 7 -0.015536873647214122 1107 8 -0.02689836170981863 1107 9 -0.15765541200875077 1107 10-11 -0.07975652934487698 1107 12-13 -0.04629360608395672 1107 14-15 -0.06445492573893574 1107 16-17 -0.08775693377732097 1107 18-19 -0.11953056878395074 1107 20-21 0.0542461520003954 1107 22-23 0.12906661796517938 1107 24-25 0.08310037795736491 1107 26-27 0.06412008123445645 1107 28-29 0.04763415658470649 1107 30-31 -0.08986260074669872 1107 32-33 0.06073235829345691 1107 34-35 0.05578609187811878 1107 36-37 0.11462884971078324 1107 38-39 0.01557599072667415 1107 40-41 0.08380546044869419 1107 42-43 -0.021169900078696458 1107 44-45 -0.030926788113049497 1107 46-47 -0.08962764998045714 1107 48-49 -0.12633487979710623 1107 50-51 -0.07746079929499672 1107 52-53 -0.11026853444603546 1107 54-55 -0.1675678148304769 1107 56-57 0.10466190434077305 1107 58-59 0.11760270050414334 1107 60-61 -0.16948453918142548 1107 62-63 0.037355211013419876 1107 64-65 0.12188402633073281 1107 66-67 0.048516463269258736 1107 68-69 0.11735199646425087 1107 70-71 0.15274740148897692 1107 72-73 0.17707198209602026 1107 74-75 0.23682093730453602 1107 76-77 0.2721588800122561 1107 78-79 0.4758024693543952 1107 80-81 0.14721536100964983 1107 82-83 0.06512243166347886 1107 84-85 0.513991497634084 1107 86-87 0.3915986327789476 1107 88-89 0.7109513435271992 1107 90-91 0.5874940586641095 1107 92-93 0.7692645811816696 1107 94-95 1.0250371037294776 1107 96-97 1.061956597939993 1107 98-99 0.8712692420715662 1107 100-101 0.984856597865825 1108 1 -0.015287228198793912 1108 2 0.020159694066066436 1108 3 -0.0364754939933718 1108 4 0.06576719260469588 1108 5 0.055353445391155276 1108 6 0.12573839961267197 1108 7 0.10953810635812289 1108 8 0.209646892888955 1108 9 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-0.9253422519811068 2316 56-57 -0.9853619430360183 2316 58-59 -0.8999767266459671 2316 60-61 -0.7710250027949428 2316 62-63 -1.0040964339427347 2316 64-65 -0.9603213111224704 2316 66-67 -1.0566908550547573 2316 68-69 -1.0009505446917117 2316 70-71 -0.8918181852077787 2316 72-73 -0.7447757729170625 2316 74-75 -0.833446287937786 2316 76-77 -0.9216835926786509 2316 78-79 -0.9676169047160705 2316 80-81 -1.2371985832951857 2316 82-83 -1.1987909420992793 2316 84-85 -1.5930083805647932 2316 86-87 -1.3178496081564859 2316 88-89 -1.4208486846793704 2316 90-91 -1.7390667585572857 2316 92-93 -1.6775291646514496 2316 94-95 -1.5256329626841847 2316 96-97 -1.4053894839103585 2316 98-99 -1.8795841670776205 2316 100-101 -1.5616001114526021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 11.0 5 62.0 6 132.0 7 257.0 8 420.0 9 763.0 10 1264.0 11 1921.0 12 2799.0 13 3756.0 14 4555.0 15 5977.0 16 8025.0 17 10904.0 18 10934.0 19 7534.0 20 7204.0 21 7794.0 22 8328.0 23 9575.0 24 10496.0 25 12399.0 26 14242.0 27 17222.0 28 20522.0 29 25086.0 30 29940.0 31 36544.0 32 44800.0 33 56994.0 34 78073.0 35 112841.0 36 164620.0 37 203915.0 38 103371.0 39 3993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.29877774694241 17.712213540546983 14.65394251853705 18.335066193973553 2 13.97974649535326 24.633716838366208 37.23000275485043 24.1565339114301 3 25.910293114139392 26.793110732332696 30.93278456972254 16.363811583805372 4 12.08483771205291 15.972194506068945 39.81586307127859 32.12710471059955 5 11.548177272191877 41.564463013455935 33.30000905304207 13.587350661310122 6 26.34503382235985 36.82524416443829 17.338327757113696 19.49139425608817 7 24.35755038309943 37.224551460703466 19.970621418329394 18.447276737867707 8 22.520950978263937 29.23412237571293 20.953995944626534 27.290930701396604 9 23.74145679995405 22.6981180380403 23.258238511640947 30.3021866503647 10-11 24.098808600195273 24.186370012430896 26.76527019436784 24.949551193005988 12-13 32.60268145265307 22.651684693252758 25.23209371553395 19.513540138560217 14-15 26.464643023151112 24.83737275163114 30.064710191307565 18.633274033910187 16-17 19.130064443276286 28.47263594416214 31.245546404999708 21.151753207561864 18-19 18.34025619285234 32.15338084423517 30.484398986442745 19.021963976469742 20-21 21.80975178116169 27.648477190714498 26.652885924855855 23.888885103267953 22-23 19.821440913325397 27.03632606137184 27.0630471371458 26.079185888156964 24-25 23.449494684009586 27.0918388206503 28.09740888055619 21.361257614783923 26-27 24.63936282158985 23.101367788108195 32.37961644305003 19.879652947251916 28-29 20.100965755164857 27.551765391875037 31.764837011172233 20.58243184178787 30-31 19.915962460052235 29.01022993753401 29.654796798143444 21.41901080427031 32-33 20.26474794091965 28.772271213565155 29.107087753180977 21.855893092334213 34-35 22.891068924241313 25.48767243716713 28.674988402369863 22.9462702362217 36-37 23.57044274541591 24.779041095556966 31.603780703612017 20.046735455415103 38-39 24.642610974598217 24.132521135700742 31.79988356380431 19.424984325896734 40-41 21.024563931202497 26.468785821892638 32.567153499623984 19.939496747280884 42-43 19.300634083586885 29.195054079877885 28.926516492646975 22.57779534388825 44-45 20.64134313799931 28.163688859555364 27.564233606485566 23.63073439595976 46-47 22.070206503746096 28.1842788090175 25.920851344267497 23.82466334296891 48-49 22.819208121076308 26.551492183522463 27.674487200329423 22.954812495071806 50-51 22.311136074698666 28.16293413441789 29.574874862986896 19.95105492789655 52-53 21.223883677170466 29.060167685701742 28.868204256097908 20.847744381029887 54-55 18.20586228278783 26.725261589110648 30.91662537635832 24.1522507517432 56-57 22.17016993436058 28.71099282668097 26.557634294091482 22.561202944866974 58-59 22.9610002000439 26.81011745156723 26.992931295384047 23.23595105300482 60-61 21.154496688525757 25.91195133858321 30.514284824263342 22.419267148627686 62-63 24.262397853717324 25.643785023234035 28.679079243953236 21.4147378790954 64-65 22.24496858422392 28.2371585672907 29.12811182643715 20.389761022048233 66-67 20.585133075720844 26.555957219907256 29.123726519306093 23.735183185065807 68-69 19.378286662162765 29.42269614838726 28.815545851188308 22.383471338261664 70-71 22.034006374126573 28.736379052213714 25.622567600980457 23.607046972679253 72-73 20.64044918663771 26.14815449356991 27.805542603323925 25.405853716468457 74-75 20.1370805788885 30.135776162074762 26.932654128041168 22.794489130995572 76-77 22.593439869366378 25.48023055743378 27.695487644950905 24.230841928248935 78-79 20.776610093098395 25.905953439607725 30.11117584727716 23.206260620016714 80-81 21.301160838139737 28.8535202355747 27.997371687230782 21.84794723905478 82-83 20.348172210275884 27.55731403056034 28.326141194359078 23.768372564804693 84-85 20.283701198701813 26.569098140912196 27.918976168086925 25.228224492299066 86-87 21.36350084473449 27.326235095356594 28.125615038807123 23.184649021101798 88-89 22.31815355617775 25.34241017659664 27.849458791509598 24.489977475716007 90-91 20.590050200925848 24.721232182335626 29.687825970123733 25.000891646614797 92-93 20.43943271496838 28.272747715080328 28.5671662712856 22.7206532986657 94-95 20.025845264256876 28.180196492150294 27.38736502420256 24.406593219390277 96-97 17.84335122201466 26.94044169110826 28.084142672049172 27.132064414827912 98-99 20.262216982922848 27.291514768626634 26.313396847399783 26.13287140105074 100-101 21.208568117448763 25.924303508267272 27.8677489490384 24.999379425245564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4949.0 1 3248.5 2 1495.5 3 1342.0 4 1085.5 5 1158.5 6 1888.0 7 3250.5 8 4524.5 9 4686.5 10 3598.5 11 2534.5 12 2257.5 13 2238.0 14 2224.5 15 2139.0 16 2062.5 17 2011.5 18 2056.5 19 2188.5 20 2296.5 21 2481.5 22 2765.0 23 3006.5 24 3283.0 25 3763.5 26 4330.5 27 4933.0 28 5395.0 29 6114.5 30 7338.0 31 8428.0 32 9461.5 33 10405.0 34 11645.0 35 12763.5 36 13714.5 37 15302.0 38 16113.0 39 15942.0 40 16211.5 41 17106.5 42 18263.0 43 20690.5 44 25943.0 45 29770.0 46 35349.0 47 64528.0 48 162914.0 49 163790.5 50 69444.0 51 37418.5 52 22339.0 53 17515.0 54 15727.0 55 14367.5 56 13129.5 57 11691.0 58 10760.0 59 10055.0 60 9215.0 61 8192.0 62 6939.0 63 5198.5 64 3556.5 65 2577.0 66 1744.0 67 1346.0 68 975.5 69 564.0 70 424.0 71 382.5 72 347.5 73 296.5 74 206.5 75 145.5 76 107.5 77 45.5 78 22.5 79 12.5 80 5.0 81 5.0 82 3.0 83 1.5 84 0.5 85 0.5 86 1.0 87 1.0 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.381882623902562 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.327394992012881E-4 16-17 0.0018495462284345344 18-19 5.840672300319582E-4 20-21 0.0 22-23 0.0 24-25 9.734453833865971E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012606117714856431 36-37 0.021561815242013126 38-39 0.010415865602236589 40-41 0.005986689107827571 42-43 0.004721210109424996 44-45 0.0012654789984025761 46-47 9.247731142172672E-4 48-49 0.0025309579968051522 50-51 4.8672269169329854E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.3805042252396866E-4 60-61 0.001703529420926545 62-63 0.0014114958059105657 64-65 8.274285758786076E-4 66-67 0.002482285727635822 68-69 0.002044235305111854 70-71 9.734453833865971E-5 72-73 0.0 74-75 0.0 76-77 0.08731805088977776 78-79 0.0031636974960064404 80-81 3.8937815335463884E-4 82-83 0.0 84-85 9.734453833865971E-5 86-87 0.0896543198099056 88-89 0.42666111153834546 90-91 0.37881627094489423 92-93 0.0 94-95 0.0011194621908945865 96-97 0.0 98-99 0.0 100-101 5.353949608626283E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1027279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.838390845453766 #Duplication Level Percentage of deduplicated Percentage of total 1 71.22537233511048 21.252504978479966 2 14.259407991935804 8.509555777763351 3 5.562864655682317 4.979607894498287 4 2.7894779714659057 3.3293413586953284 5 1.612298524127963 2.405419676123922 6 1.0187681513550972 1.823904136862027 7 0.674122988192357 1.4080321639712134 8 0.48383307434889145 1.154944030110377 9 0.3575206184556459 0.9601055953909122 >10 1.790489450552056 9.535505739214805 >50 0.11537340670404186 2.4078391945364106 >100 0.07582613325470976 4.427536764484659 >500 0.011439287344459495 2.204991326982232 >1k 0.015034491938432478 10.604535687957583 >5k 0.005883062062864883 12.156221535087804 >10k+ 0.002287857468891899 12.839954139841117 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 29949 2.9153715787045194 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 25901 2.521320887509625 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 24835 2.4175516096406136 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15960 1.5536188318850088 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13843 1.3475404442220662 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11011 1.071860711646982 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10162 0.9892151985974599 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9937 0.9673126774712614 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9828 0.9567021227923476 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8659 0.8429063574744544 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7957 0.7745704915607152 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7634 0.7431282056773282 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7132 0.694261247431321 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7062 0.6874471297476148 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6895 0.6711905918450586 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6766 0.6586331463993715 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6608 0.6432527093418633 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6534 0.6360492135048025 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6162 0.5998370452428211 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6023 0.5863061544137473 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5990 0.5830937846485716 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5755 0.5602178181389866 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5424 0.5279967759488903 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5254 0.5114482044313181 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5030 0.4896430278434583 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4823 0.4694927084073558 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4626 0.4503158343546398 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4620 0.44973176712460783 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4549 0.442820304902563 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4397 0.42802393507508674 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4250 0.41371428793930376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4060 0.3952188256549584 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3633 0.3536527077843507 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3505 0.34119260687700226 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3429 0.33379442196326414 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3409 0.3318475311964909 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 3282 0.3194847748274811 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3237 0.3151042706022415 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2934 0.2856088754856276 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2911 0.2833699511038384 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2861 0.2785027241869054 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2711 0.2639010434361065 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2581 0.2512462534520807 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2462 0.2396622533897802 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2374 0.23109593401597814 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2325 0.2263260516373838 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2284 0.22233492556549875 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 2088 0.20325539605112147 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2006 0.19527314390735134 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1943 0.1891404379920158 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1834 0.17852988331310188 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1638 0.15945035379872458 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1498 0.14582211843131224 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1498 0.14582211843131224 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1431 0.13930003436262203 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1399 0.1361850091357849 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1395 0.1357956309824303 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1354 0.13180450491054524 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1321 0.12859213514536946 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1304 0.12693727799361226 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 1294 0.12596383261022565 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1256 0.12226474015335659 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1238 0.12051253846326071 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1215 0.11827361408147154 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1178 0.11467186616294113 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1137 0.11068074009105608 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1114 0.10844181570926692 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 1101 0.10717633671086432 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1089 0.1060082022508004 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1085 0.10561882409744579 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1060 0.10318521063897929 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4601680750798954E-4 0.0 14-15 0.0 0.0 0.0 1.946890766773194E-4 0.0 16-17 0.0 0.0 0.0 3.407058841853089E-4 0.0 18-19 0.0 0.0 0.0 5.840672300319582E-4 0.0 20-21 0.0 0.0 0.0 9.734453833865971E-4 0.0 22-23 0.0 0.0 0.0 0.003017680688498451 0.0 24-25 0.0 0.0 0.0 0.005499966416134273 0.0 26-27 0.0 0.0 0.0 0.008955697527156692 0.0 28-29 0.0 0.0 0.0 0.023362689201278326 0.0 30-31 0.0 0.0 0.0 0.05704389946645459 0.0 32-33 0.0 0.0 0.0 0.1136497485103852 0.0 34-35 0.0 0.0 0.0 0.21089694231070624 0.0 36-37 0.0 0.0 0.0 0.3415333127611876 0.0 38-39 0.0 0.0 0.0 0.5147092464656632 0.0 40-41 0.0 0.0 0.0 0.6952833650838769 0.0 42-43 0.0 0.0 0.0 0.8764415509321226 0.0 44-45 0.0 0.0 0.0 1.0765332494872375 0.0 46-47 0.0 0.0 0.0 1.342624545035964 0.0 48-49 0.0 0.0 0.0 1.620640546531176 0.0 50-51 0.0 0.0 0.0 1.9053246489025863 0.0 52-53 0.0 0.0 0.0 2.1566682468930054 0.0 54-55 0.0 0.0 0.0 2.4177949709864603 0.0 56-57 0.0 0.0 0.0 2.7366470063147403 0.0 58-59 0.0 0.0 0.0 3.117361495757238 0.0 60-61 0.0 0.0 0.0 3.645309599436959 0.0 62-63 0.0 0.0 0.0 4.1207403246829735 0.0 64-65 0.0 0.0 0.0 4.521459116754066 0.0 66-67 0.0 0.0 0.0 4.98092533771254 0.0 68-69 0.0 0.0 0.0 5.495585911909034 0.0 70-71 0.0 0.0 0.0 6.068312503224538 0.0 72-73 0.0 0.0 0.0 6.689614019171033 0.0 74-75 0.0 0.0 0.0 7.351070157182226 0.0 76-77 0.0 0.0 0.0 7.89688098364709 0.0 78-79 0.0 0.0 0.0 8.610659811015314 0.0 80-81 0.0 0.0 0.0 9.37856220169983 0.0 82-83 0.0 0.0 0.0 10.095018003872365 0.0 84-85 0.0 0.0 0.0 11.081118177242988 0.0 86-87 0.0 0.0 0.0 12.12411623327256 0.0 88-89 0.0 0.0 0.0 12.992721548868419 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 295 0.0 38.607555 94-95 GTCGGGA 280 0.0 32.200123 2 CCATGCG 75 3.0953356E-4 31.63675 9 TTACTCG 30 0.0039699976 31.636747 94-95 ACCACTC 605 0.0 29.022137 94-95 CGGGAGT 300 0.0 28.471685 4 TATGACC 135 4.2612555E-7 28.120182 4 TTTGCGT 1080 0.0 26.36267 3 CTTTGCG 1070 0.0 26.165567 2 ATGGGAG 1055 0.0 25.188221 5 GCTTTGC 1190 0.0 24.019318 1 GAGTACT 4910 0.0 23.343233 12-13 TCGGGAG 390 0.0 23.118036 3 TTGCGTT 1245 0.0 22.868824 4 GTACTTT 5345 0.0 21.62104 14-15 TAGGACC 155 3.9359526E-5 21.4303 4 GTGTAGC 245 7.2686817E-9 21.388628 1 TATATGG 245 7.603376E-9 21.305344 2 TAGGACA 135 3.8211263E-4 21.090137 4 GGTCGGG 410 0.0 20.91438 1 >>END_MODULE