##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938894_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036358 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.855427371622547 33.0 31.0 34.0 30.0 34.0 2 31.841857736419268 33.0 31.0 34.0 30.0 34.0 3 31.89732795038008 34.0 31.0 34.0 30.0 34.0 4 35.38050750802329 37.0 35.0 37.0 33.0 37.0 5 35.44905042466021 37.0 35.0 37.0 33.0 37.0 6 35.58761933617534 37.0 35.0 37.0 33.0 37.0 7 35.537098184218195 37.0 35.0 37.0 33.0 37.0 8 35.54339427109165 37.0 35.0 37.0 33.0 37.0 9 37.08785477605229 39.0 37.0 39.0 33.0 39.0 10-11 36.99031464030769 39.0 37.0 39.0 33.0 39.0 12-13 36.89666312220295 39.0 37.0 39.0 33.0 39.0 14-15 37.88091952780796 40.0 37.5 41.0 33.0 41.0 16-17 37.854173461294266 40.0 37.5 41.0 32.5 41.0 18-19 37.944612286487875 40.0 38.0 41.0 33.0 41.0 20-21 37.80115703260842 40.0 37.5 41.0 32.5 41.0 22-23 37.94241613419301 40.0 38.0 41.0 33.0 41.0 24-25 37.84134536521164 40.0 37.5 41.0 32.5 41.0 26-27 37.60730124146289 40.0 37.0 41.0 32.5 41.0 28-29 37.47370020784324 40.0 37.0 41.0 32.0 41.0 30-31 37.385452710356844 40.0 37.0 41.0 32.0 41.0 32-33 37.20295255114546 40.0 36.0 41.0 31.5 41.0 34-35 36.86320315952595 39.0 35.5 41.0 30.5 41.0 36-37 36.49396106364789 39.0 35.0 41.0 30.0 41.0 38-39 36.18019014664817 39.0 35.0 40.5 30.0 41.0 40-41 36.02830151356964 39.0 35.0 40.0 30.0 41.0 42-43 35.907775594919904 39.0 35.0 40.0 29.5 41.0 44-45 35.86116718354082 39.0 35.0 41.0 29.0 41.0 46-47 35.545010507951886 39.0 35.0 41.0 27.0 41.0 48-49 35.303574151017315 39.0 35.0 41.0 26.0 41.0 50-51 34.053602616084405 38.0 34.0 40.0 23.5 40.5 52-53 34.141419277894315 38.0 33.5 39.5 23.5 40.5 54-55 34.70259553165991 38.0 34.5 40.0 24.0 41.0 56-57 34.664328349855936 38.5 34.5 40.0 23.0 41.0 58-59 34.549396540577675 38.0 34.0 40.0 23.0 41.0 60-61 34.15407465373934 38.0 34.0 40.0 22.0 41.0 62-63 33.76896497156388 37.5 33.5 40.0 22.0 41.0 64-65 33.20375729236422 37.0 33.0 39.5 20.0 41.0 66-67 32.70258250527327 36.0 32.0 39.0 18.0 40.5 68-69 32.338282234517415 36.0 32.0 39.0 18.0 40.0 70-71 31.72552486688963 35.0 31.5 37.5 13.0 39.5 72-73 31.167008408291345 35.0 31.0 37.0 9.0 39.0 74-75 30.73116143263235 35.0 31.0 37.0 7.0 39.0 76-77 30.22103848284087 34.5 30.5 36.0 2.0 37.5 78-79 29.752345232053017 34.0 29.5 35.5 2.0 37.0 80-81 29.348036103354247 34.0 29.0 35.0 2.0 36.5 82-83 28.961947994804884 34.0 29.0 35.0 2.0 36.0 84-85 28.597505398713572 34.0 29.0 35.0 2.0 36.0 86-87 28.224698415026467 34.0 29.0 35.0 2.0 35.0 88-89 27.651867404892904 34.0 26.5 35.0 2.0 35.0 90-91 27.077965336302704 33.0 25.5 35.0 2.0 35.0 92-93 26.726160265082143 33.0 25.0 35.0 2.0 35.0 94-95 26.23566036060898 33.0 24.5 35.0 2.0 35.0 96-97 25.48194928779437 33.0 21.5 35.0 2.0 35.0 98-99 24.769062910693023 32.0 10.0 35.0 2.0 35.0 100-101 22.199461479527343 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25504575729743095 1101 2 0.41897430023794513 1101 3 0.40319172275911797 1101 4 0.1799182100422314 1101 5 0.02555392337245621 1101 6 0.12330092129426617 1101 7 0.1176750452072639 1101 8 0.3594887857523119 1101 9 0.13391938233939982 1101 10-11 0.3437249215763174 1101 12-13 0.4722915468571074 1101 14-15 0.28717367145902273 1101 16-17 0.34429497108892804 1101 18-19 0.39015679489196486 1101 20-21 0.6245258880989439 1101 22-23 0.3600700994075723 1101 24-25 0.5837783419463349 1101 26-27 0.398929713258525 1101 28-29 0.4040590852594548 1101 30-31 0.3850305838831716 1101 32-33 0.4140831820089943 1101 34-35 0.36471326433068185 1101 36-37 0.45574241011075856 1101 38-39 0.5378085323913027 1101 40-41 0.5692724170472729 1101 42-43 0.5295412475362582 1101 44-45 0.5363709127618534 1101 46-47 0.592051279069274 1101 48-49 0.6488376695472269 1101 50-51 0.34365125562324295 1101 52-53 0.22473480396242707 1101 54-55 0.11929821493836101 1101 56-57 0.14887376362296578 1101 58-59 0.1347823279426379 1101 60-61 0.2184572250240251 1101 62-63 0.2933277394775047 1101 64-65 0.3981999232742126 1101 66-67 0.17574314457558415 1101 68-69 0.16755416929977685 1101 70-71 0.34735609301452186 1101 72-73 0.20543559352122998 1101 74-75 0.18681943127217338 1101 76-77 -0.051845356169948786 1101 78-79 9.605504193253012E-4 1101 80-81 0.11542685208255321 1101 82-83 0.021690593088443677 1101 84-85 -0.23446453246518217 1101 86-87 -0.32416445444453856 1101 88-89 -0.145077592558696 1101 90-91 -0.1007315335646446 1101 92-93 0.06228426695497902 1101 94-95 -0.059301890715289574 1101 96-97 -0.35414395648989583 1101 98-99 -0.5239377843725883 1101 100-101 -0.143016474985874 1102 1 0.3334525432092903 1102 2 0.13749418468075092 1102 3 0.2503243062000209 1102 4 0.28693154829289114 1102 5 0.28777040871267445 1102 6 0.3320414708056134 1102 7 0.22720851376710272 1102 8 0.22731102436562622 1102 9 0.41955463957709327 1102 10-11 0.2458024924165656 1102 12-13 0.4385156540548394 1102 14-15 0.5131740402585763 1102 16-17 0.591316914662599 1102 18-19 0.6259549386008629 1102 20-21 0.6649217329572679 1102 22-23 0.6808949810843714 1102 24-25 0.7781971753104031 1102 26-27 0.7809937614483289 1102 28-29 0.6079130406347133 1102 30-31 0.77156940003659 1102 32-33 0.6817947807550766 1102 34-35 0.7376802567702825 1102 36-37 0.8478869795372788 1102 38-39 0.7608277714348759 1102 40-41 0.7649390222473471 1102 42-43 0.605993395793682 1102 44-45 0.7671287958523862 1102 46-47 0.7770226971035683 1102 48-49 0.611871660573108 1102 50-51 0.5432947493843798 1102 52-53 0.8270582411742993 1102 54-55 0.8657485191980001 1102 56-57 0.8343615010376837 1102 58-59 0.8044854442988978 1102 60-61 0.5868879829071147 1102 62-63 0.6218482385862387 1102 64-65 0.7657455622194433 1102 66-67 0.8585202052431171 1102 68-69 0.7272935509458449 1102 70-71 0.5963080876054612 1102 72-73 0.566809986538626 1102 74-75 0.588835535519511 1102 76-77 0.5975369045713421 1102 78-79 0.47989594903017974 1102 80-81 0.3219300176120896 1102 82-83 0.36350631624291196 1102 84-85 0.6063492852249013 1102 86-87 0.6077428539333596 1102 88-89 0.7657589677707612 1102 90-91 0.7464809564470336 1102 92-93 0.5255026577595778 1102 94-95 0.37883813018335033 1102 96-97 0.5966397425696677 1102 98-99 0.6567186788396526 1102 100-101 0.4809309096105956 1103 1 0.14306072613081255 1103 2 0.28648462452576595 1103 3 0.34182725602381225 1103 4 0.35410250826745227 1103 5 0.309629608982668 1103 6 0.19278642300483284 1103 7 0.34812532808721386 1103 8 0.23539139976234225 1103 9 0.3410030063028202 1103 10-11 0.384023966095306 1103 12-13 0.34503155195005064 1103 14-15 0.5044121874187582 1103 16-17 0.3604534502908905 1103 18-19 0.5762298011695748 1103 20-21 0.5525055384571118 1103 22-23 0.536543455548447 1103 24-25 0.4962959181467781 1103 26-27 0.5289266132042769 1103 28-29 0.5186946628106384 1103 30-31 0.6743890940856616 1103 32-33 0.6388714372320337 1103 34-35 0.5163136673377267 1103 36-37 0.34383543388153726 1103 38-39 0.5812308815681249 1103 40-41 0.574887943890424 1103 42-43 0.5023487895253922 1103 44-45 0.5023926574280893 1103 46-47 0.3427593093169463 1103 48-49 0.31081439699709534 1103 50-51 0.27687276557109897 1103 52-53 0.4592129430441432 1103 54-55 0.49643642199983873 1103 56-57 0.6097887224918921 1103 58-59 0.5041632028222338 1103 60-61 0.5214985996766544 1103 62-63 0.40205716009950976 1103 64-65 0.24593079427873477 1103 66-67 0.28568324709880955 1103 68-69 0.14594623758954128 1103 70-71 0.2305039766739121 1103 72-73 0.23968621076417307 1103 74-75 0.22722088829766918 1103 76-77 0.18946009881837256 1103 78-79 0.4208540414125572 1103 80-81 0.30017790665457866 1103 82-83 0.3552896453575727 1103 84-85 0.16650589523158743 1103 86-87 0.3685653647558702 1103 88-89 0.3245153038884183 1103 90-91 0.25950528843688403 1103 92-93 0.1848045003447787 1103 94-95 0.33829833514580443 1103 96-97 0.2042693693147335 1103 98-99 0.41155230098961937 1103 100-101 0.6529861157737464 1104 1 0.2091293742152729 1104 2 0.278928116475182 1104 3 0.2761863751655369 1104 4 0.39284966361540086 1104 5 0.42164045715952625 1104 6 0.20764705644254633 1104 7 0.22225696061806133 1104 8 0.26884707452236967 1104 9 0.3472762126748421 1104 10-11 0.17552487296183017 1104 12-13 0.2618212016942465 1104 14-15 0.41674343324802976 1104 16-17 0.3557619132376928 1104 18-19 0.47175621151812663 1104 20-21 0.4437286105102203 1104 22-23 0.4771094771421289 1104 24-25 0.5482289982419104 1104 26-27 0.5078687020771895 1104 28-29 0.5242887887110825 1104 30-31 0.6581467725073722 1104 32-33 0.5817484712652004 1104 34-35 0.519046030494998 1104 36-37 0.6271510148749826 1104 38-39 0.570240472109333 1104 40-41 0.7579391647380831 1104 42-43 0.6284534983870103 1104 44-45 0.7349793230681172 1104 46-47 0.7803534181067064 1104 48-49 0.8626137998340582 1104 50-51 0.8385771185303952 1104 52-53 0.8250811821831334 1104 54-55 1.0718079378357928 1104 56-57 0.8420738122374516 1104 58-59 0.8768885505969806 1104 60-61 0.8040794904591237 1104 62-63 0.8794239961619965 1104 64-65 0.6700095975571472 1104 66-67 0.8517518951832699 1104 68-69 0.881403221317278 1104 70-71 0.8270777750757752 1104 72-73 0.7697464831663439 1104 74-75 0.7075778172711971 1104 76-77 0.7071300935140599 1104 78-79 0.6533487370989555 1104 80-81 0.5115376931131799 1104 82-83 0.4077200048531502 1104 84-85 0.4943456279626268 1104 86-87 0.5108075922253725 1104 88-89 0.48664336028524957 1104 90-91 0.6724332695466835 1104 92-93 0.6533999694343144 1104 94-95 0.725512158871485 1104 96-97 1.100504803840682 1104 98-99 1.1352382000707735 1104 100-101 1.1660501268614745 1105 1 0.057033386960469556 1105 2 0.18335159551531532 1105 3 0.1830867037526147 1105 4 -0.113157837463298 1105 5 0.09617329927177565 1105 6 0.07509204300034611 1105 7 0.07542583446053897 1105 8 0.12271710007914294 1105 9 0.1764316775093846 1105 10-11 -0.018492933802420453 1105 12-13 0.09130234942566062 1105 14-15 0.11088777922699222 1105 16-17 0.15752060088082942 1105 18-19 0.24545016086924676 1105 20-21 0.10728365714303578 1105 22-23 0.2072448948460064 1105 24-25 0.2092159211401139 1105 26-27 0.16420686464481804 1105 28-29 0.26591023316660056 1105 30-31 0.23529420521340683 1105 32-33 0.1931631381973844 1105 34-35 0.24791398163841194 1105 36-37 0.30542402426792137 1105 38-39 0.2869731051207154 1105 40-41 0.15611658516358773 1105 42-43 0.16855398296997492 1105 44-45 -0.029576835450257022 1105 46-47 0.40186218042872923 1105 48-49 0.30843219739122674 1105 50-51 0.30744224847164503 1105 52-53 0.14586638912660987 1105 54-55 0.36329827936719994 1105 56-57 0.3379809946862551 1105 58-59 0.35503875471925994 1105 60-61 0.38000716811188084 1105 62-63 0.475718264143012 1105 64-65 0.37557358906035176 1105 66-67 0.43559968302886887 1105 68-69 0.5343351971545047 1105 70-71 0.42754368831551304 1105 72-73 0.5855448004022286 1105 74-75 0.6155568408033467 1105 76-77 0.4576009853458487 1105 78-79 0.4347325367604107 1105 80-81 0.4954029060309715 1105 82-83 0.47656356089549945 1105 84-85 0.3459566173748172 1105 86-87 0.5677191226445686 1105 88-89 0.5520247204365987 1105 90-91 0.7165477439733152 1105 92-93 0.7586059171922912 1105 94-95 0.7427609059258202 1105 96-97 0.5083240975444205 1105 98-99 0.7242222353855894 1105 100-101 1.117533747293617 1106 1 -0.19047057310289262 1106 2 -0.12385331976333092 1106 3 -0.1773630634436607 1106 4 -0.09844960213153087 1106 5 -0.06078521206440257 1106 6 -0.03598173418009054 1106 7 -0.11300118037170392 1106 8 -0.12186281319947057 1106 9 -0.043083834738808946 1106 10-11 -0.13461679178414698 1106 12-13 -0.09810068102282798 1106 14-15 -0.14838036906338203 1106 16-17 -0.12837797349636304 1106 18-19 -0.21309117579213677 1106 20-21 0.025488348418093665 1106 22-23 -0.06167340784683262 1106 24-25 0.026827002917421794 1106 26-27 -0.06001472290058274 1106 28-29 -0.03918664838350594 1106 30-31 -0.050440366539739045 1106 32-33 -0.018106242112700954 1106 34-35 -0.24588391912799779 1106 36-37 -0.2390926879994737 1106 38-39 -0.403963949872157 1106 40-41 -0.2078032375535699 1106 42-43 -0.27621913339989135 1106 44-45 -0.1557408540745513 1106 46-47 -0.24266813205627358 1106 48-49 -0.34337423151163904 1106 50-51 -0.30443554157299957 1106 52-53 -0.5108581184655137 1106 54-55 -0.8633913476668269 1106 56-57 -0.7007816255156669 1106 58-59 -0.7257525379414247 1106 60-61 -0.6411231699735254 1106 62-63 -0.7212188868514033 1106 64-65 -0.764370650047475 1106 66-67 -0.5154775342066031 1106 68-69 -0.5313198533262984 1106 70-71 -0.5407258039963629 1106 72-73 -0.3236917473825365 1106 74-75 -0.30791393472906137 1106 76-77 -0.31403936089969164 1106 78-79 -0.2648606567306864 1106 80-81 -0.27867747925519737 1106 82-83 -0.23006974523739743 1106 84-85 -0.15956147091459272 1106 86-87 -0.25432403004587556 1106 88-89 -0.07203926458716126 1106 90-91 -0.26582197394217033 1106 92-93 -0.1554267073296991 1106 94-95 -0.10977273461243087 1106 96-97 -0.09048948347287578 1106 98-99 -0.09538887839223165 1106 100-101 0.44558899617759806 1107 1 0.03009809375593875 1107 2 -0.08493861189510099 1107 3 0.053801475625039785 1107 4 -0.006744075378286141 1107 5 -0.0014013402177965872 1107 6 0.10485728832256314 1107 7 0.08591277727639834 1107 8 0.08601528787492185 1107 9 -0.18421208684436152 1107 10-11 0.044298370164689516 1107 12-13 -0.10320810069991637 1107 14-15 -0.03628366174005038 1107 16-17 0.025329298505816666 1107 18-19 -0.08264467635875405 1107 20-21 -0.1299676274800987 1107 22-23 0.023471147726702668 1107 24-25 0.05113364157586631 1107 26-27 0.1703961410766084 1107 28-29 0.11351621706253212 1107 30-31 0.17014212016359664 1107 32-33 0.15314976458400054 1107 34-35 -0.10299659824305962 1107 36-37 -0.07205765666790853 1107 38-39 -0.17717585941930025 1107 40-41 -0.14274138387808932 1107 42-43 -0.168621279029459 1107 44-45 0.2537243574282968 1107 46-47 0.1454932955731465 1107 48-49 0.06532299148931742 1107 50-51 -0.0011837321604986073 1107 52-53 -0.1882600074408245 1107 54-55 -0.11369044858722788 1107 56-57 0.04493965191179683 1107 58-59 0.02612548021390637 1107 60-61 -0.19844837855446684 1107 62-63 -0.4247925999277413 1107 64-65 -0.25983922174680174 1107 66-67 -0.20686313155951552 1107 68-69 -0.1788002982463226 1107 70-71 -0.13258433617093957 1107 72-73 -0.1957802022844355 1107 74-75 -0.1774486797924162 1107 76-77 -0.06648843477267974 1107 78-79 -0.08218896490532401 1107 80-81 -0.23766166145602696 1107 82-83 -0.29195451537389516 1107 84-85 -0.3429088544231753 1107 86-87 -0.22425402266213013 1107 88-89 -0.10738845257258234 1107 90-91 -0.1224499713677325 1107 92-93 0.049183987650536665 1107 94-95 0.21311904810803028 1107 96-97 0.26566863923944695 1107 98-99 0.21269296667546556 1107 100-101 0.5921716447705414 1108 1 0.026040367262631037 1108 2 0.0358807597914641 1108 3 -0.08014479366563165 1108 4 0.04522569667324916 1108 5 0.1091896503905403 1108 6 0.033150467173229003 1108 7 0.007200552379835301 1108 8 -0.009562016386723826 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-2.770461294191282 2316 90-91 -3.080424829560595 2316 92-93 -3.3078914274388396 2316 94-95 -3.615086683308423 2316 96-97 -4.001340865435001 2316 98-99 -4.162884732985084 2316 100-101 -4.792476784005597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3881.0 3 757.0 4 753.0 5 835.0 6 957.0 7 1190.0 8 1446.0 9 1853.0 10 2409.0 11 3283.0 12 4521.0 13 6684.0 14 6721.0 15 6484.0 16 8349.0 17 11328.0 18 9437.0 19 6711.0 20 6850.0 21 7295.0 22 7926.0 23 8761.0 24 10111.0 25 11601.0 26 13580.0 27 15633.0 28 19003.0 29 22271.0 30 26615.0 31 32521.0 32 39895.0 33 50640.0 34 68515.0 35 102567.0 36 145519.0 37 203413.0 38 148133.0 39 17908.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.36718989654428 17.045178533930592 14.165043367018615 18.42258820250651 2 13.683051559534675 23.916824817342004 37.89222574741285 24.507897875710473 3 25.779055706443 26.85588338697028 30.90444252991242 16.4606183766743 4 11.801573440084082 15.27081175906892 40.664751387193 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28.234018685087005 21.577999947823212 26-27 24.664959274295434 22.79890599341103 32.41939045029454 20.11674428199899 28-29 20.092918188612234 27.389464602054208 31.991799006083593 20.525818203249962 30-31 19.953433101142917 28.782546107992697 29.79450665172404 21.469514139140347 32-33 20.22979578055648 28.576665975936503 29.153932838809183 22.039605404697834 34-35 22.89569595362004 25.32691427505891 28.665195901553993 23.112193869767058 36-37 23.463346619539703 24.59960602426654 31.771682375385396 20.165364980808363 38-39 24.73738173140516 23.95970461176555 31.70396544002298 19.59894821680631 40-41 20.98529072940035 26.347953677317648 32.78792214879039 19.878833444491605 42-43 19.375070654122535 29.004360299393962 28.848763546790863 22.77180549969264 44-45 20.525706632114545 28.18710555624304 27.684110149442777 23.603077662199638 46-47 21.986832860398042 27.977163242252857 26.078855328382716 23.95714856896639 48-49 22.660922167766937 26.470219946942496 27.77661295880521 23.09224492648536 50-51 22.39381152622652 27.850267275872863 29.801830045059578 19.954091152841038 52-53 21.23139550910351 28.812719483005296 29.06716932197829 20.888715685912903 54-55 18.333766022647037 26.466076753422897 31.21142493891802 23.988732285012045 56-57 22.164211506788174 28.50820232813679 26.810103308341077 22.517482856733963 58-59 22.852210005054822 26.606846421700226 27.30811808297088 23.232825490274074 60-61 21.225950127834455 25.449505511209335 30.859087301260214 22.465457059696 62-63 24.312658210036453 25.330248667238898 28.973864927142717 21.383228195581932 64-65 22.320009280386593 27.91115097664905 29.524831586708274 20.244008156256083 66-67 20.760685150620027 26.257715468801955 29.44448624625908 23.53711313431894 68-69 19.49025625540975 29.22264127495161 29.19526179684136 22.091840672797275 70-71 22.176617990149484 28.39192735512822 26.051143838283025 23.38031081643927 72-73 20.828627081894357 25.989283580307376 28.216102976260103 24.96598636153816 74-75 20.42688890452881 29.914112573443578 27.411558277980152 22.24744024404746 76-77 23.02290594972338 25.31228768319907 27.86775696399107 23.79704940308648 78-79 21.185753606502256 25.828247380364267 30.477242634768658 22.50875637836482 80-81 21.884650223151368 28.832544051714642 28.241225680527975 21.041580044606015 82-83 20.883291716847445 27.43708761588485 28.65933129601792 23.020289371249785 84-85 21.077191545364375 26.498428683180215 28.19392708857025 24.23045268288517 86-87 22.285329151292945 27.345313352186157 28.435238219087417 21.93411927743348 88-89 23.306161648955815 25.4412668925231 27.917389150058646 23.335182308462436 90-91 21.43886456417671 24.857315784594523 29.94667330641517 23.757146344813595 92-93 21.63563518667913 28.44484376501699 28.71729753815955 21.20222351014433 94-95 21.22654628854345 28.51515041197957 27.546543376169502 22.711759923307486 96-97 19.331928979837496 26.94611359731296 28.101453214642813 25.62050420820673 98-99 21.870783102704443 27.58892010538967 26.24718226315963 24.293114528746255 100-101 23.117415174431272 26.16461816422411 27.626223484199592 23.09174317714503 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6349.0 1 3932.5 2 1578.5 3 1520.0 4 1187.5 5 1179.5 6 1932.5 7 3442.5 8 4885.5 9 4998.0 10 3764.0 11 2660.5 12 2428.0 13 2394.0 14 2389.0 15 2545.5 16 2883.5 17 2846.5 18 2488.0 19 2378.5 20 2429.5 21 2655.0 22 2902.5 23 3184.5 24 3452.5 25 3825.0 26 4439.5 27 5134.0 28 5708.5 29 6343.5 30 7371.5 31 8386.0 32 9410.5 33 10397.5 34 11724.5 35 12917.5 36 13600.5 37 14980.0 38 16070.0 39 16137.5 40 16114.5 41 16830.5 42 18110.5 43 20580.5 44 25993.0 45 30275.5 46 37316.0 47 66401.0 48 168450.5 49 166412.5 50 62096.5 51 31306.0 52 21284.5 53 18362.5 54 16652.0 55 14948.0 56 13393.0 57 11752.5 58 10725.0 59 9862.0 60 9103.0 61 8377.0 62 7105.0 63 5328.0 64 3644.0 65 2654.0 66 1908.0 67 1434.0 68 1065.5 69 694.5 70 500.0 71 424.0 72 405.5 73 350.0 74 256.5 75 182.0 76 143.5 77 86.0 78 47.0 79 25.0 80 17.0 81 13.5 82 13.0 83 10.0 84 6.0 85 3.5 86 0.5 87 1.0 88 1.5 89 1.0 90 3.0 91 3.0 92 1.5 93 2.0 94 1.0 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28648401421130537 2 0.09195664046593938 3 0.09668473635558368 4 0.11328131784576372 5 0.11569361166701081 6 0.08742152808199484 7 0.09919352192968066 8 0.09465840954573614 9 0.09986896419962987 10-11 0.09296980387086315 12-13 0.0975049162548077 14-15 0.10744356679834575 16-17 0.0950926224335606 18-19 0.10671987865197162 20-21 0.10995235237244273 22-23 0.12336470601857659 24-25 0.13653583028258573 26-27 0.1395753204973571 28-29 0.13137352150511694 30-31 0.12428137767065048 32-33 0.1178646761061332 34-35 0.38746263356870886 36-37 0.5877795124850679 38-39 0.5709899474891881 40-41 0.5350950154290313 42-43 0.5605688381814007 44-45 0.5818935155612249 46-47 0.5706522263542134 48-49 0.5939067387910355 50-51 0.584305809382472 52-53 0.5882137253728924 54-55 0.5770689279187308 56-57 0.5505336958850128 58-59 0.5460468293774932 60-61 0.49611234727767817 62-63 0.5678057196451419 64-65 0.600998882625502 66-67 0.6142182527659361 68-69 0.6165340548343333 70-71 0.6137840398781116 72-73 0.6037006517052987 74-75 0.6011918661312018 76-77 0.5845470387645968 78-79 0.561919722721299 80-81 0.557674085595904 82-83 0.5709417016127631 84-85 0.5801084181335021 86-87 0.5712311768713129 88-89 0.5844987928881719 90-91 0.5981041300400055 92-93 0.6054857491330217 94-95 0.6050515362451971 96-97 0.6009506367490771 98-99 0.6055822408858715 100-101 0.5840163341239224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1036358.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.489056357052757 #Duplication Level Percentage of deduplicated Percentage of total 1 73.19840665325668 23.049487523508652 2 13.236693283671954 8.336219615811357 3 5.1853314380843845 4.898435816515097 4 2.606160079851144 3.282620865197351 5 1.4880673416500207 2.3428918192153594 6 0.9689290075147039 1.8306396074168219 7 0.6493823408863428 1.4313905990611424 8 0.4697546414639743 1.1833704303236956 9 0.3216305613455922 0.9115058585125679 >10 1.6650616385066246 9.28548180617991 >50 0.10267846019665125 2.1882502750155646 >100 0.07808477665889185 4.82440982853934 >500 0.007685509513665884 1.547892392365503 >1k 0.014756178266238498 10.888682431398156 >5k 0.004918726088746166 10.341063465568784 >10k+ 0.002459363044373083 13.657657665370815 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 29642 2.860208537976259 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24481 2.362214601518008 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 23542 2.271608845591967 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17466 1.6853249552760727 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14709 1.4192971926689426 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10669 1.0294705111554117 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10373 1.000908952311846 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10204 0.9846018460802155 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9914 0.9566192377537492 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8571 0.8270308136763551 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8271 0.7980832878213899 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7852 0.7576532433772886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7585 0.7318899453663695 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6900 0.6657930946641991 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6426 0.620056003813354 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6118 0.5903365439355898 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6005 0.5794329758635529 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5925 0.5717136356355622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5808 0.5604241005521258 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5702 0.5501959747500381 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5685 0.54855561495159 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5573 0.537748538632403 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5309 0.5122747158800337 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5181 0.4999237715152486 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4837 0.46673060853488857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4605 0.4443445218737154 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4564 0.4403883600068702 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4489 0.4331514785431289 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4404 0.4249496795508888 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4220 0.4071951970265102 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4129 0.3984144475171707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4015 0.3874143876922839 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3619 0.34920365356372995 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3566 0.3440895906626861 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 3555 0.34302818138133734 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3387 0.3268175669025568 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3270 0.3155280318191204 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3095 0.2986419750703907 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3050 0.29429984619214594 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3039 0.2932384369107972 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3016 0.2910191265952499 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2650 0.2557031450521924 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2530 0.2441241347102063 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2334 0.22521175115162909 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2282 0.2201941800034351 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 2142 0.20668533460445135 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2112 0.20379058201895484 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2107 0.2033081232547054 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1975 0.19057121187852075 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1814 0.17503603966968945 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1773 0.1710798778028442 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1684 0.16249211179920453 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1521 0.14676395608467344 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1435 0.13846566533958343 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1391 0.13422002821418855 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1391 0.13422002821418855 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1320 0.1273691137618468 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1285 0.12399190241210083 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 1268 0.1223515426136528 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1258 0.12138662508515397 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.1200357405452556 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1241 0.11974626528670596 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1240 0.11964977353385606 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1210 0.11675502094835954 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1099 0.10604443638202242 No Hit GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA 1077 0.10392161781932498 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 1066 0.10286020853797626 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1051 0.101412832245228 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.10064089822242892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.649175284988392E-5 0.0 12-13 0.0 0.0 0.0 2.8947525854965177E-4 0.0 14-15 0.0 0.0 0.0 4.342128878244777E-4 0.0 16-17 0.0 0.0 0.0 5.789505170993035E-4 0.0 18-19 0.0 0.0 0.0 8.201798992240133E-4 0.0 20-21 0.0 0.0 0.0 0.001399130416323317 0.0 22-23 0.0 0.0 0.0 0.0029429984619214595 0.0 24-25 0.0 0.0 0.0 0.004824587642494196 0.0 26-27 0.0 0.0 0.0 0.008201798992240134 0.0 28-29 0.0 0.0 0.0 0.0231097748075472 0.0 30-31 0.0 0.0 0.0 0.0596801491376532 0.0 32-33 0.0 0.0 0.0 0.11916731476960664 0.0 34-35 0.0 0.0 0.0 0.2172029356650887 0.0 36-37 0.0 0.0 0.0 0.3561510597689216 0.0 38-39 0.0 0.0 0.0 0.52776164221244 0.0 40-41 0.0 0.0 0.0 0.713990725212716 0.0 42-43 0.0 0.0 0.0 0.9013777092471906 0.0 44-45 0.0 0.0 0.0 1.1136595655169352 0.0 46-47 0.0 0.0 0.0 1.3811829502932382 0.0 48-49 0.0 0.0 0.0 1.6787635160822805 0.0 50-51 0.0 0.0 0.0 1.9671291194741587 0.0 52-53 0.0 0.0 0.0 2.2227357727735013 0.0 54-55 0.0 0.0 0.0 2.4950354993158737 0.0 56-57 0.0 0.0 0.0 2.8225767543648046 0.0 58-59 0.0 0.0 0.0 3.2037191781218457 0.0 60-61 0.0 0.0 0.0 3.735822949212531 0.0 62-63 0.0 0.0 0.0 4.211189569627484 0.0 64-65 0.0 0.0 0.0 4.611726835707352 0.0 66-67 0.0 0.0 0.0 5.063983681314758 0.0 68-69 0.0 0.0 0.0 5.5653548291227555 0.0 70-71 0.0 0.0 0.0 6.133980728667121 0.0 72-73 0.0 0.0 0.0 6.755966567537472 0.0 74-75 0.0 0.0 0.0 7.420071056526798 0.0 76-77 0.0 0.0 0.0 7.978468830269076 0.0 78-79 0.0 0.0 0.0 8.712336856568868 0.0 80-81 0.0 0.0 0.0 9.489915646909658 0.0 82-83 0.0 0.0 0.0 10.19681422828791 0.0 84-85 0.0 0.0 0.0 11.185372236234969 0.0 86-87 0.0 0.0 0.0 12.235588474253106 0.0 88-89 0.0 0.0 0.0 13.099865104529517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 130 0.0 29.27163 94-95 CGTCACT 135 4.286976E-7 28.100868 1 GCGCCGT 45 8.877268E-4 26.425777 94-95 CTTTGCG 945 0.0 25.543648 2 CGGGAGT 380 0.0 24.912191 4 TTTGCGT 975 0.0 24.757692 3 GAGTACT 4660 0.0 24.325045 12-13 TTGCGTT 1045 0.0 24.006294 4 GCTTTGC 1035 0.0 23.82465 1 GTCGGGA 390 0.0 23.058632 2 AGTACTT 4715 0.0 22.686152 12-13 ATGGGAG 1165 0.0 22.346128 5 GTACTTT 5180 0.0 21.930424 14-15 GTACTAA 135 3.8389149E-4 21.075651 1 GGTCGGG 435 0.0 20.712278 1 GTATAGA 115 0.0037594622 20.617485 1 CCTAAGG 210 8.905736E-7 20.284662 3 TCGGGAG 450 0.0 19.98415 3 GTCGCGG 60 0.0047250823 19.820295 92-93 CATGGGG 1150 0.0 19.75645 4 >>END_MODULE