##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938894_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036358 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04829991180654 33.0 31.0 34.0 30.0 34.0 2 32.250990487843005 34.0 31.0 34.0 30.0 34.0 3 32.34595960083292 34.0 31.0 34.0 30.0 34.0 4 35.87348869792099 37.0 35.0 37.0 35.0 37.0 5 35.79962619094946 37.0 35.0 37.0 35.0 37.0 6 35.90430044444101 37.0 35.0 37.0 35.0 37.0 7 35.86774647370889 37.0 35.0 37.0 35.0 37.0 8 35.87098956152218 37.0 35.0 37.0 35.0 37.0 9 37.46966588765658 39.0 37.0 39.0 34.0 39.0 10-11 37.38853417448411 39.0 37.0 39.0 34.0 39.0 12-13 37.32725660437802 39.0 37.0 39.0 34.0 39.0 14-15 38.30269366377256 40.0 37.5 41.0 33.0 41.0 16-17 38.34686324609835 40.0 37.5 41.0 33.5 41.0 18-19 38.62574129789127 40.0 38.0 41.0 34.0 41.0 20-21 38.63052825375016 40.0 38.0 41.0 34.0 41.0 22-23 38.60721922347297 40.0 38.0 41.0 34.0 41.0 24-25 38.49191833324006 40.0 38.0 41.0 34.0 41.0 26-27 38.14483846315655 40.0 37.5 41.0 33.5 41.0 28-29 37.93972546166479 40.0 37.0 41.0 33.0 41.0 30-31 37.872745228965286 40.0 37.0 41.0 33.0 41.0 32-33 37.7240784555144 40.0 36.5 41.0 33.0 41.0 34-35 37.665477566632376 40.0 36.5 41.0 33.0 41.0 36-37 37.46335050243256 40.0 36.0 41.0 32.5 41.0 38-39 37.22635180121155 39.5 35.5 41.0 31.5 41.0 40-41 37.05958848197245 39.0 35.5 41.0 31.0 41.0 42-43 36.96325256330341 39.0 35.5 41.0 31.0 41.0 44-45 36.766206272349905 39.5 35.0 41.0 31.0 41.0 46-47 36.41512344189942 39.0 35.0 41.0 30.0 41.0 48-49 36.07634909944247 39.0 35.0 41.0 29.5 41.0 50-51 35.802017256585074 39.0 35.0 41.0 28.0 41.0 52-53 35.45226070527752 39.0 35.0 40.0 28.0 41.0 54-55 35.29273474996092 38.0 35.0 40.0 27.0 41.0 56-57 35.08134013535863 38.0 34.5 40.0 26.5 41.0 58-59 34.874783134785474 38.0 34.0 40.0 26.0 41.0 60-61 34.30848751107243 38.0 34.0 40.0 25.5 41.0 62-63 33.948693887633425 37.0 33.0 40.0 25.0 41.0 64-65 33.5543745501072 36.5 33.0 39.0 23.0 40.5 66-67 33.037491388111064 36.0 32.0 39.0 22.0 40.0 68-69 32.620354163329665 35.5 32.0 38.0 22.0 40.0 70-71 32.00993479087342 35.0 31.5 37.0 20.5 39.0 72-73 31.408828319943495 35.0 31.0 37.0 19.0 39.0 74-75 30.970616331422153 34.5 31.0 36.0 18.0 38.0 76-77 28.911034121413643 32.5 28.0 34.5 11.5 36.0 78-79 29.855295177921143 34.0 29.0 35.0 11.0 36.5 80-81 29.926073326012826 34.0 30.0 35.0 8.5 36.0 82-83 29.635137182325025 34.0 30.0 35.0 6.5 36.0 84-85 29.290492764083453 34.0 29.0 35.0 2.0 35.5 86-87 28.768702031537366 34.0 29.0 35.0 2.0 35.0 88-89 28.100266992680133 33.5 28.0 35.0 2.0 35.0 90-91 27.532408685029694 33.0 26.5 35.0 2.0 35.0 92-93 27.306033243338693 33.0 26.0 35.0 2.0 35.0 94-95 26.941510559092514 33.0 25.5 35.0 2.0 35.0 96-97 26.20854135347052 32.0 25.0 34.0 2.0 35.0 98-99 25.339372108865852 32.0 21.5 34.0 2.0 35.0 100-101 21.111468720268476 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08289989543911247 1101 2 0.02840362618188408 1101 3 0.15661292631333623 1101 4 0.052083591751717506 1101 5 0.11887999438738461 1101 6 0.08834427017580992 1101 7 0.05574595410697469 1101 8 0.03971055650457345 1101 9 0.048822318138448395 1101 10-11 0.08045063316616563 1101 12-13 0.10670323506629842 1101 14-15 0.21220408975433003 1101 16-17 0.08861598467493792 1101 18-19 0.2027894190986146 1101 20-21 0.1450449992613443 1101 22-23 -0.057187667971035694 1101 24-25 0.18124619587126034 1101 26-27 0.1557776631241765 1101 28-29 0.3202741803792577 1101 30-31 0.2635339066471758 1101 32-33 0.05016438556388181 1101 34-35 0.17014681920731078 1101 36-37 0.2504466009326336 1101 38-39 0.4225158899407617 1101 40-41 0.35962769213328727 1101 42-43 0.21837881090630873 1101 44-45 0.1674466421669436 1101 46-47 0.0847044065519853 1101 48-49 -0.009218811734861276 1101 50-51 -0.0643398947828473 1101 52-53 0.04143842997927294 1101 54-55 -0.06380037158007212 1101 56-57 0.05892152052157229 1101 58-59 0.18125357626879435 1101 60-61 0.3526380499794399 1101 62-63 0.054064105764801695 1101 64-65 -0.24808443574936234 1101 66-67 -0.014470270364931537 1101 68-69 -0.09635108347146115 1101 70-71 0.024103946910848606 1101 72-73 0.004263780153273444 1101 74-75 -0.1775979074158336 1101 76-77 -0.4360059026362535 1101 78-79 -0.18563598916463775 1101 80-81 -0.07234480421493217 1101 82-83 -0.3981051110860534 1101 84-85 -0.5087034192512299 1101 86-87 -0.15752758414648227 1101 88-89 0.046792695937128315 1101 90-91 0.22199447151961493 1101 92-93 -0.0019933281276500736 1101 94-95 -0.0807853917372725 1101 96-97 -0.22240020043897957 1101 98-99 -0.1574038918005023 1101 100-101 0.4799089076621357 1102 1 0.23721792359081206 1102 2 0.1709200684954908 1102 3 0.17894988617565133 1102 4 0.12660568899187297 1102 5 0.1568540554851765 1102 6 0.25536129642204486 1102 7 0.14173771758353126 1102 8 0.2631539150392115 1102 9 0.23163625876171778 1102 10-11 0.30318097922307885 1102 12-13 0.351727514370495 1102 14-15 0.3339632636433194 1102 16-17 0.3061401103126542 1102 18-19 0.3048669899388585 1102 20-21 0.40704635152638247 1102 22-23 0.48119821929923035 1102 24-25 0.4154277681867029 1102 26-27 0.3808007130965123 1102 28-29 0.48238002585224393 1102 30-31 0.46503983817338934 1102 32-33 0.4427084878442926 1102 34-35 0.5113048498176838 1102 36-37 0.5678477933845372 1102 38-39 0.5825011379584666 1102 40-41 0.6040188655305414 1102 42-43 0.51197399192543 1102 44-45 0.5613614494691745 1102 46-47 0.7365023043945129 1102 48-49 0.679778668134837 1102 50-51 0.47771554808394967 1102 52-53 0.46384300309377835 1102 54-55 0.41603858594650944 1102 56-57 0.5113709642489113 1102 58-59 0.5718614808726485 1102 60-61 0.6980434221596639 1102 62-63 0.7667692505186281 1102 64-65 0.6088828025566215 1102 66-67 0.6440705127194661 1102 68-69 0.6566945862070668 1102 70-71 0.8359055327489457 1102 72-73 0.5187698960792702 1102 74-75 0.5821599142081162 1102 76-77 0.38733296269044004 1102 78-79 0.3535428171541284 1102 80-81 0.7929818907263666 1102 82-83 0.8410798418920038 1102 84-85 0.7127545683325174 1102 86-87 0.783870166176218 1102 88-89 0.7696459751798557 1102 90-91 0.8146492136672592 1102 92-93 0.8664374297554396 1102 94-95 1.1036635602573206 1102 96-97 1.0433813472898272 1102 98-99 1.0764273209353767 1102 100-101 1.780585040184107 1103 1 0.12982032368946506 1103 2 0.10157487096438444 1103 3 0.08065163270497777 1103 4 0.022949724766192503 1103 5 -0.07792141392297225 1103 6 -0.046471553386815856 1103 7 0.0638448458375862 1103 8 0.054810035031167104 1103 9 0.09336124042673077 1103 10-11 0.10655364354027341 1103 12-13 0.13764786588305356 1103 14-15 0.2112973238859226 1103 16-17 0.18069147099594574 1103 18-19 0.07131168966692059 1103 20-21 0.1703741032349484 1103 22-23 0.12605598522146977 1103 24-25 0.20693623473265177 1103 26-27 0.1639773066179302 1103 28-29 0.3182364482903708 1103 30-31 0.37206846671625726 1103 32-33 0.2689723039881713 1103 34-35 0.2386879115335745 1103 36-37 0.2453807801379213 1103 38-39 0.48774656719190546 1103 40-41 0.5032543127420013 1103 42-43 0.5989353813605831 1103 44-45 0.4685604473728233 1103 46-47 0.6441994298577569 1103 48-49 0.6041346719511083 1103 50-51 0.6788211641071058 1103 52-53 0.6171478441797333 1103 54-55 0.9295123685357325 1103 56-57 0.821923480198727 1103 58-59 0.7913215560298781 1103 60-61 0.8232827505474134 1103 62-63 0.7611892483119433 1103 64-65 0.7040916916185864 1103 66-67 0.966798758985945 1103 68-69 0.9127824513653948 1103 70-71 0.9194887411447539 1103 72-73 0.998210370647179 1103 74-75 1.042863004221548 1103 76-77 0.8803640445521062 1103 78-79 0.9044881237216238 1103 80-81 0.8866542104106792 1103 82-83 0.8820285233954088 1103 84-85 0.9397952687615252 1103 86-87 0.768100453629895 1103 88-89 1.0159903354672437 1103 90-91 1.0238384502178093 1103 92-93 1.0824333279102625 1103 94-95 1.156323918389944 1103 96-97 0.8558484903416819 1103 98-99 1.0358047750654542 1103 100-101 1.2425504860326129 1104 1 0.09842007045126877 1104 2 0.14109200731946459 1104 3 0.09314671338187708 1104 4 -0.022320880781094843 1104 5 0.036247519909991865 1104 6 0.09738656477960461 1104 7 -0.09410820243166995 1104 8 0.11176938003399073 1104 9 0.11176592533339402 1104 10-11 -0.03243220591988205 1104 12-13 0.04676764926173149 1104 14-15 0.09240583987585893 1104 16-17 0.06847743338665424 1104 18-19 0.06516788288082154 1104 20-21 0.0421038227907502 1104 22-23 0.0847058663021798 1104 24-25 0.10781401308078387 1104 26-27 0.09149108072261924 1104 28-29 0.16784953283428905 1104 30-31 0.14319482059952549 1104 32-33 0.16405597525761095 1104 34-35 -0.012722650894772869 1104 36-37 -0.08619656873192127 1104 38-39 0.15046922003313767 1104 40-41 0.30985861094709577 1104 42-43 0.11941794556843632 1104 44-45 0.08734819783053638 1104 46-47 -0.07758290506687615 1104 48-49 -0.015101164676032397 1104 50-51 -0.1455686259447333 1104 52-53 -0.2285907387130024 1104 54-55 -0.18460737206621758 1104 56-57 -0.040002883950954526 1104 58-59 -0.18525711891836494 1104 60-61 0.08025107866198766 1104 62-63 0.22770897693640535 1104 64-65 -0.056269900017710484 1104 66-67 0.19610338058305388 1104 68-69 0.15855290291648316 1104 70-71 0.017741525909393374 1104 72-73 0.019060814669558113 1104 74-75 -0.11831861718734515 1104 76-77 -0.2917363353051883 1104 78-79 -0.17247361677766904 1104 80-81 -0.06131539245022921 1104 82-83 0.08753538478171663 1104 84-85 0.07874796626165548 1104 86-87 0.1744729019983886 1104 88-89 0.16343100415297585 1104 90-91 0.4503063334544706 1104 92-93 0.24685207780331808 1104 94-95 0.34666963986214583 1104 96-97 0.5193657208055491 1104 98-99 0.7404935316316816 1104 100-101 1.3568413335250398 1105 1 -0.09802272552602886 1105 2 -0.08237209703182202 1105 3 -0.03136787389284024 1105 4 -0.06071412317214708 1105 5 0.06337669777848731 1105 6 -0.01954396726593899 1105 7 -0.18663533575836766 1105 8 0.04983984370686301 1105 9 -0.07407246956852731 1105 10-11 -0.11340070802748414 1105 12-13 -0.17016412743182485 1105 14-15 -0.08669900927279883 1105 16-17 -0.07367381623995328 1105 18-19 0.01035980760450883 1105 20-21 0.012257163426021123 1105 22-23 -0.12836542713603194 1105 24-25 -0.06189374356564059 1105 26-27 -0.06744839619330634 1105 28-29 -0.07144027100918748 1105 30-31 0.056059087584827694 1105 32-33 0.03163787309690491 1105 34-35 0.1649114391845501 1105 36-37 -0.0678753499725886 1105 38-39 -0.05067419660863948 1105 40-41 -0.044040456396665206 1105 42-43 -0.07435093159309503 1105 44-45 0.0040003436037139295 1105 46-47 0.12735253918949496 1105 48-49 -0.033721098958615414 1105 50-51 0.04364069066052423 1105 52-53 0.12555725823346364 1105 54-55 -0.16839790610319483 1105 56-57 -0.18929218993522312 1105 58-59 -0.14183342413924294 1105 60-61 0.0021784575992214172 1105 62-63 -0.15310145487347881 1105 64-65 -0.20750500572982133 1105 66-67 -0.014623825762988929 1105 68-69 -0.08060413847221781 1105 70-71 0.024539378476688967 1105 72-73 -0.022624521717311552 1105 74-75 0.1454160736420924 1105 76-77 -0.155175629672744 1105 78-79 -0.06888986026401156 1105 80-81 -0.01798297185877118 1105 82-83 0.11140565926809032 1105 84-85 0.08346310781962174 1105 86-87 0.09091968084932844 1105 88-89 0.1664306058656102 1105 90-91 0.10748670784459335 1105 92-93 -0.018108591319453637 1105 94-95 -0.07478062400323182 1105 96-97 -0.18855895309667048 1105 98-99 -0.061001705515252524 1105 100-101 0.9338186643358881 1106 1 -0.20834049061027216 1106 2 -0.29603551564652264 1106 3 -0.24523529577158598 1106 4 -0.20077236410400445 1106 5 -0.3161733750226716 1106 6 -0.20898299829043054 1106 7 -0.10953410213862469 1106 8 -0.1139187764310634 1106 9 -0.27367166315562486 1106 10-11 -0.23032657884142083 1106 12-13 -0.2482773772438165 1106 14-15 -0.2246112158681015 1106 16-17 -0.3156636880778976 1106 18-19 -0.25737047608003394 1106 20-21 -0.24760099430450566 1106 22-23 -0.19766053585777854 1106 24-25 -0.22041027983021166 1106 26-27 -0.2082006482293437 1106 28-29 -0.0900465687099441 1106 30-31 -0.1248878075655 1106 32-33 -0.13553080160189523 1106 34-35 -0.2871517938271779 1106 36-37 -0.5353668298362635 1106 38-39 -0.42082411900739913 1106 40-41 -0.30096256715043523 1106 42-43 -0.4516469683296904 1106 44-45 -0.676116538266406 1106 46-47 -0.5834108330244661 1106 48-49 -0.65830109223851 1106 50-51 -0.6505384162051797 1106 52-53 -0.6167406185325675 1106 54-55 -0.6465735569136015 1106 56-57 -0.7259347762166115 1106 58-59 -0.608314887842667 1106 60-61 -0.7627117806445867 1106 62-63 -0.6484413803122848 1106 64-65 -0.6256596962048633 1106 66-67 -0.8590273362191567 1106 68-69 -0.9042393166350635 1106 70-71 -0.7871196080670977 1106 72-73 -0.5969277105683304 1106 74-75 -0.6328863791971564 1106 76-77 -0.7558612406425205 1106 78-79 -0.6094365090103899 1106 80-81 -0.38481684120067783 1106 82-83 -0.4768537528825014 1106 84-85 -0.6527166710568402 1106 86-87 -0.5700448945838765 1106 88-89 -0.1849770166563509 1106 90-91 -0.1917020791759967 1106 92-93 -0.4300271820169037 1106 94-95 -0.3005102356770166 1106 96-97 -0.5809952320771323 1106 98-99 -0.6149565687930654 1106 100-101 -0.19316936747262048 1107 1 -0.025285791665709212 1107 2 -0.06598643545588345 1107 3 -0.12495033057785321 1107 4 -0.021819197582772176 1107 5 -0.014409173158178135 1107 6 -0.035098753308020036 1107 7 -0.06778928754171432 1107 8 -0.06418115605034558 1107 9 -0.0448564976531145 1107 10-11 -0.03995131083514991 1107 12-13 0.015845023144713366 1107 14-15 -0.0566606198379489 1107 16-17 -0.01767181019794606 1107 18-19 -0.02791021997857257 1107 20-21 -0.103472355243305 1107 22-23 -0.04919542701662749 1107 24-25 -0.11732707252070185 1107 26-27 -0.16886048657664787 1107 28-29 -0.08647364533901225 1107 30-31 -0.1817653518975817 1107 32-33 -0.21642772925303433 1107 34-35 -0.06419278743451429 1107 36-37 -0.08765964075315935 1107 38-39 -0.18164505725638236 1107 40-41 -0.2860929080267667 1107 42-43 -0.3244708363857072 1107 44-45 -0.21086406944989022 1107 46-47 -0.23140409288887298 1107 48-49 -0.1957030206991064 1107 50-51 -0.10978172049051693 1107 52-53 -0.03474649394074447 1107 54-55 -0.22664228102608064 1107 56-57 -0.14404430045858163 1107 58-59 0.04597429832643485 1107 60-61 0.09436782955682332 1107 62-63 -0.09588846017913966 1107 64-65 -0.26904551862381254 1107 66-67 -0.2856414940575789 1107 68-69 -0.2750523973153136 1107 70-71 -0.0985122930259763 1107 72-73 0.039280183326852125 1107 74-75 0.002216914269215664 1107 76-77 -0.368152412920697 1107 78-79 -0.05658991718147277 1107 80-81 -0.07681791520002434 1107 82-83 0.12980731345669838 1107 84-85 -0.0727086555470251 1107 86-87 -0.23172769756422795 1107 88-89 0.021476159736195655 1107 90-91 0.22170721723597353 1107 92-93 0.36725297387094 1107 94-95 -0.0233340050198656 1107 96-97 -5.731147979837203E-4 1107 98-99 -0.12625429167754376 1107 100-101 0.5615506029923978 1108 1 0.0144390262429539 1108 2 0.008622835077567004 1108 3 -0.09097535142200286 1108 4 -0.11222970932628584 1108 5 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-2.7734840181733382 2316 96-97 -3.0453163757302697 2316 98-99 -3.6289990356537665 2316 100-101 -4.394769143189322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 23.0 5 47.0 6 140.0 7 260.0 8 399.0 9 731.0 10 1246.0 11 1806.0 12 2656.0 13 3762.0 14 4417.0 15 5826.0 16 7828.0 17 10732.0 18 10357.0 19 7034.0 20 6504.0 21 7044.0 22 7446.0 23 8104.0 24 9466.0 25 11091.0 26 12753.0 27 15537.0 28 18807.0 29 22850.0 30 27971.0 31 33742.0 32 41641.0 33 52817.0 34 71682.0 35 106417.0 36 158979.0 37 223982.0 38 134347.0 39 7909.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.31811158033224 17.76068172372402 14.588934196338524 18.332272499605214 2 13.951260085800465 24.686932507878552 37.12134223887884 24.24046516744214 3 25.877544246293272 26.80907562830605 30.958028017345356 16.355352108055325 4 12.081056932064016 15.90145490264947 39.910436355004734 32.107051810281774 5 11.570263085525713 41.602829146383236 33.22133824799417 13.605569520096877 6 26.379976803382615 36.91677972283709 17.26276055185563 19.440482921924662 7 24.33985167287752 37.2841238259366 19.896695929398913 18.47932857178697 8 22.46868360161257 29.198114937116326 20.925876965295775 27.407324495975327 9 23.843305112712017 22.810746865465408 23.24206500070439 30.103883021118182 10-11 24.092495064446844 24.24972837571573 26.677653860924504 24.980122698912925 12-13 32.599642208580434 22.700167316699442 25.16726845356527 19.532922021154853 14-15 26.511975282314832 24.85398174156626 30.010575649181785 18.623467326937124 16-17 19.164666819127902 28.464850448071992 31.184980831376908 21.185501901423194 18-19 18.326003176508504 32.204315497154454 30.430941817402868 19.03873950893417 20-21 21.843175813763196 27.66418554206172 26.581162108074626 23.91147653610046 22-23 19.912327593360597 27.07076126203493 26.925203452860885 26.091707691743586 24-25 23.575009793912916 27.11678782814433 28.00706898581378 21.301133392128975 26-27 24.6477568562215 23.07580971054404 32.34562766920311 19.930805764031348 28-29 20.072841624226378 27.589693908861612 31.738308576765945 20.599155890146072 30-31 19.99632366421642 28.965087353983854 29.569897660846927 21.4686913209528 32-33 20.257719822686752 28.79564783597946 29.077162524918993 21.869469816414792 34-35 22.926859863991528 25.502384611116607 28.59680652686276 22.973948998029105 36-37 23.5539851805103 24.805733638080017 31.559230915094076 20.0810502663156 38-39 24.727096386617674 24.085194469307844 31.740440592829206 19.447268551245276 40-41 21.04364742016757 26.51219161519482 32.528198145952004 19.91596281868561 42-43 19.385168509835975 29.173425168548434 28.845109128295583 22.596297193320012 44-45 20.62591546903857 28.217385179195524 27.52794894408667 23.62875040767923 46-47 22.060522784937763 28.180876505941267 25.922139501627584 23.83646120749339 48-49 22.800595742094043 26.57869483849397 27.594949411615428 23.02576000779656 50-51 22.36396970135572 28.095768803975492 29.61156945047523 19.928692044193564 52-53 21.27807186319785 29.079381835234543 28.815139170055136 20.82740713151247 54-55 18.252379969084043 26.70269347078905 30.866746819149366 24.178179740977537 56-57 22.158896829088018 28.695537642397706 26.56639886988859 22.579166658625688 58-59 22.967768268483418 26.87289918652353 26.948211253967635 23.211121291025417 60-61 21.17853670774924 26.019573928126004 30.379537356675375 22.422352007449383 62-63 24.229223878235125 25.69261780195647 28.660125169101796 21.41803315070661 64-65 22.22885872656632 28.30659942461829 29.069561554891155 20.394980293924235 66-67 20.626135908034087 26.59135679827238 29.05497758699964 23.72752970669389 68-69 19.455062747294686 29.41227271564965 28.7442001390471 22.388464398008566 70-71 22.07369461132157 28.782717941097573 25.56963906294929 23.573948384631567 72-73 20.678809832123648 26.106036717041793 27.778142302177432 25.437011148657124 74-75 20.16648687036719 30.252190845248457 26.837106482508936 22.744215801875413 76-77 22.61183633405598 25.49595008714269 27.629593347013383 24.262620231787952 78-79 20.74583336348702 25.947899252912244 30.07847195586465 23.227795427736087 80-81 21.323567724666574 28.860297310388884 27.936533514480516 21.87960145046403 82-83 20.37814153024341 27.549215618541083 28.3490840037902 23.72355884742531 84-85 20.309246418708593 26.54208294817042 27.94729234492328 25.201378288197706 86-87 21.37148323694395 27.410048653689127 28.08076415764375 23.13770395172317 88-89 22.306569300624517 25.46943130407733 27.777425142708186 24.44657425258997 90-91 20.596245185949652 24.766969951031744 29.72314248814519 24.913642374873415 92-93 20.500734302239188 28.329447932085245 28.525181452741233 22.64463631293433 94-95 20.116938355278773 28.21722802352083 27.38103049332374 24.28480312787666 96-97 17.883819509375666 26.91264462977383 28.08618298977279 27.11735287107771 98-99 20.264618983015524 27.357390013875516 26.338437103780738 26.039553899328226 100-101 21.217340541270747 26.213975389766563 27.725664165116765 24.843019903845924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5136.0 1 3318.5 2 1453.5 3 1328.0 4 1120.5 5 1155.0 6 1858.5 7 3300.0 8 4594.5 9 4732.5 10 3585.0 11 2502.0 12 2294.5 13 2269.0 14 2230.5 15 2138.0 16 2100.5 17 2096.5 18 2094.5 19 2172.0 20 2184.0 21 2383.5 22 2744.5 23 2985.0 24 3312.5 25 3731.0 26 4283.5 27 4859.0 28 5405.5 29 6237.0 30 7362.0 31 8400.0 32 9471.5 33 10470.0 34 11667.5 35 12781.5 36 13734.0 37 15261.5 38 16189.0 39 16177.0 40 16344.0 41 17301.0 42 18365.5 43 20695.0 44 26325.5 45 30207.5 46 35506.5 47 64878.0 48 166213.5 49 166371.5 50 69195.0 51 37720.0 52 22299.5 53 17546.5 54 16064.0 55 14409.0 56 13106.5 57 11821.5 58 10862.5 59 10065.0 60 9189.0 61 8236.0 62 7014.5 63 5187.5 64 3492.5 65 2557.5 66 1786.0 67 1375.5 68 1018.5 69 592.0 70 424.5 71 393.5 72 351.5 73 300.0 74 220.0 75 158.0 76 115.5 77 45.5 78 16.0 79 10.0 80 7.0 81 5.0 82 3.5 83 3.5 84 2.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40053726607986817 2 0.0 3 0.0 4 0.0 5 2.8947525854965177E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014473762927482588 16-17 0.0027982608326466334 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00246053969767204 36-37 0.008443028374364843 38-39 0.013557091275408691 40-41 0.0029912443383464014 42-43 8.684257756489554E-4 44-45 3.859670113995357E-4 46-47 4.342128878244777E-4 48-49 3.377211349745937E-4 50-51 7.719340227990714E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.377211349745937E-4 60-61 0.00294299846192146 62-63 0.0 64-65 2.4122938212470978E-4 66-67 0.0016886056748729684 68-69 0.0017850974277228524 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12293049313075212 78-79 4.824587642494196E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1440621870048767 88-89 0.4288575955413091 90-91 0.3323175968150002 92-93 0.0 94-95 0.0 96-97 4.8245876424941956E-4 98-99 0.0 100-101 4.824587642494196E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1036358.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.886591710084243 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56748015968047 21.389080592519253 2 13.945886874036706 8.335900540787161 3 5.502241787679802 4.933297613956508 4 2.8230922712651556 3.3749042428478435 5 1.6106764010215027 2.4068813987198787 6 0.9953296906959986 1.784820724965345 7 0.6969239580129286 1.4580077250275805 8 0.4989997703231452 1.193072191925892 9 0.36384613597073323 0.9786708819944191 >10 1.7657955283093503 9.461876621396886 >50 0.11551084611890643 2.384066172937691 >100 0.08056636726478317 4.683904827491277 >500 0.010677470360391489 2.091351853007957 >1k 0.014883746562969957 10.555200851314446 >5k 0.00550051503414107 11.171089321828513 >10k+ 0.0025884776631252097 13.797874439279298 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 30151 2.90932284017685 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26283 2.536092740153499 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 24723 2.38556560570768 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 16265 1.5694383601033617 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14043 1.35503368527092 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11013 1.0626636741357716 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10164 0.9807421759662202 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10044 0.9691631656242341 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9720 0.9378998377008717 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8687 0.8382238570069416 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8034 0.7752147423959674 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7728 0.7456882660239029 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7284 0.7028459277585545 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7085 0.6836440689414276 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6837 0.6597141142346563 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6760 0.6522842492652152 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6732 0.6495824801854185 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6660 0.6426350739802269 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6301 0.6079945347071186 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6108 0.589371626407091 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5932 0.5723890779055114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5924 0.5716171438827123 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5517 0.5323450004728096 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5181 0.4999237715152486 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5032 0.48554650034061586 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4889 0.4717481796830825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4885 0.47136221267168293 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4566 0.44058134351257 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4484 0.4326690197788795 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4386 0.4232128279995908 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4137 0.39918638153996977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4100 0.39561618668452414 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3767 0.3634844329855127 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3699 0.35692299379172066 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3464 0.3342474318719979 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3366 0.32479124009270927 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 3333 0.3216070122486631 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3221 0.31079993592947613 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2915 0.2812734595574116 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2889 0.27876467398331467 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2841 0.2741330698465202 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2750 0.26535232033718076 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2559 0.24692239554285297 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2510 0.24219429965320863 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2360 0.22772053672572606 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2261 0.21816785319358753 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2250 0.21710644391223882 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 2108 0.2034046150075553 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2016 0.19452737374536597 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1894 0.18275537989768015 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1878 0.181211511852082 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1597 0.15409732930126463 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1594 0.15380785404271496 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1575 0.1519745107385672 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1453 0.14020251689088134 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.1308428168644426 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1334 0.12871999830174513 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 1313 0.12669367149189759 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1302 0.12563226221054888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.1248603281877498 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1264 0.12196557560225328 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1247 0.12032521580380524 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1242 0.11984275703955583 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1213 0.1170444962069092 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1203 0.11607957867841036 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1127 0.10874620546181919 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1127 0.10874620546181919 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 1121 0.10816725494471988 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1104 0.10652689514627185 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1094 0.105561977617773 No Hit GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA 1065 0.10276371678512639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.649175284988392E-5 0.0 12-13 0.0 0.0 0.0 2.8947525854965177E-4 0.0 14-15 0.0 0.0 0.0 4.342128878244777E-4 0.0 16-17 0.0 0.0 0.0 5.789505170993035E-4 0.0 18-19 0.0 0.0 0.0 8.201798992240133E-4 0.0 20-21 0.0 0.0 0.0 0.001399130416323317 0.0 22-23 0.0 0.0 0.0 0.0029429984619214595 0.0 24-25 0.0 0.0 0.0 0.004872833518919138 0.0 26-27 0.0 0.0 0.0 0.008394782497939901 0.0 28-29 0.0 0.0 0.0 0.023495741818946734 0.0 30-31 0.0 0.0 0.0 0.060307345531177455 0.0 32-33 0.0 0.0 0.0 0.12056644518592996 0.0 34-35 0.0 0.0 0.0 0.2204354093855598 0.0 36-37 0.0 0.0 0.0 0.36179582731063975 0.0 38-39 0.0 0.0 0.0 0.534516064911932 0.0 40-41 0.0 0.0 0.0 0.7220960324521063 0.0 42-43 0.0 0.0 0.0 0.9119435561842528 0.0 44-45 0.0 0.0 0.0 1.1276508696801684 0.0 46-47 0.0 0.0 0.0 1.3988409410647673 0.0 48-49 0.0 0.0 0.0 1.698303096034382 0.0 50-51 0.0 0.0 0.0 1.9882608133482829 0.0 52-53 0.0 0.0 0.0 2.246038531086748 0.0 54-55 0.0 0.0 0.0 2.519640896292594 0.0 56-57 0.0 0.0 0.0 2.848677773510698 0.0 58-59 0.0 0.0 0.0 3.2315570488190373 0.0 60-61 0.0 0.0 0.0 3.7642880163032464 0.0 62-63 0.0 0.0 0.0 4.242018684663022 0.0 64-65 0.0 0.0 0.0 4.6441963105413375 0.0 66-67 0.0 0.0 0.0 5.098286499452891 0.0 68-69 0.0 0.0 0.0 5.60182871170001 0.0 70-71 0.0 0.0 0.0 6.172625675683499 0.0 72-73 0.0 0.0 0.0 6.798085217656447 0.0 74-75 0.0 0.0 0.0 7.465373934489819 0.0 76-77 0.0 0.0 0.0 8.02584628091837 0.0 78-79 0.0 0.0 0.0 8.763477485579308 0.0 80-81 0.0 0.0 0.0 9.54235891458357 0.0 82-83 0.0 0.0 0.0 10.254564542368563 0.0 84-85 0.0 0.0 0.0 11.244328697226248 0.0 86-87 0.0 0.0 0.0 12.293773001221584 0.0 88-89 0.0 0.0 0.0 13.160413679442819 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 40 5.4140575E-4 47.45342 5 TCGCGTT 55 4.9917908E-5 43.13947 4 GTAAGCG 140 0.0 42.371166 94-95 GAGTCGT 50 0.0016235494 37.962734 6 ATACGAG 50 0.0016235494 37.962734 2 TTCGCGT 80 4.5192303E-4 29.658388 3 CATATAG 145 2.42253E-8 29.453844 3 TACGAGT 65 0.0058718943 29.202103 3 AGTCGTT 65 0.0058718943 29.202103 7 GTCGGGA 290 0.0 24.54487 2 ATAAGAC 155 1.4225134E-6 24.492086 3 CGGGAGT 295 0.0 24.128857 4 GCTTCGC 160 1.8055307E-6 23.830185 1 GTGTAGC 180 1.8881656E-7 23.830183 1 CGATACT 120 1.7220748E-4 23.72671 4 ACCACCG 100 0.001663006 23.726707 5 GAGTACT 5020 0.0 22.878159 12-13 CGCGTTG 130 2.960815E-4 21.901577 5 ATGGGAG 1005 0.0 21.71997 5 GTCGTTC 110 0.002893604 21.569735 8 >>END_MODULE