FastQCFastQC Report
Thu 26 May 2016
SRR938893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449609
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.3180507583046927No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67310.27477854629044884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.26040890607439804No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39380.1607603499170684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38500.15716793986305572No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28000.1143039562640405No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25240.10303685200372793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79900.022.0382351
GTATCAA173850.021.9498251
GAGTACT71350.021.62855512-13
TACATGG78900.021.368842
GTACTTT72800.021.00290714-15
AGTACTT75250.019.87853812-13
GTATAGG5500.019.8479121
ACATGGG82600.019.7236063
AGAGTAC119150.019.68501310-11
TCAACGC192850.019.376054
CAACGCA193000.019.3364545
TATCAAC193950.019.3149972
ATCAACG194200.019.2413563
AACGCAG196800.019.0500036
GGTATCA134450.018.2859971
ACGCAGA209350.017.5462277
CGCAGAG208800.017.3884098
ACTTTTT90600.017.2438416-17
TACTTTT87450.017.1866914-15
GTGTAGC15150.016.917291
Produced by FastQC (version 0.11.5)