##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938893_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2449609 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.586672403636662 33.0 31.0 34.0 30.0 34.0 2 31.681273623668105 33.0 31.0 34.0 30.0 34.0 3 31.72242794666414 33.0 31.0 34.0 28.0 34.0 4 35.25235047715778 37.0 35.0 37.0 33.0 37.0 5 35.286590227256674 37.0 35.0 37.0 33.0 37.0 6 35.35731090145407 37.0 35.0 37.0 33.0 37.0 7 35.27237775498049 37.0 35.0 37.0 32.0 37.0 8 35.25097229802797 37.0 35.0 37.0 32.0 37.0 9 36.827869264033566 39.0 37.0 39.0 33.0 39.0 10-11 36.69720453345819 39.0 37.0 39.0 32.0 39.0 12-13 36.57195842275237 39.0 37.0 39.0 32.0 39.0 14-15 37.7349856650592 40.0 38.0 41.0 32.0 41.0 16-17 37.630540833251345 40.0 37.0 41.0 32.0 41.0 18-19 37.52347374621827 40.0 37.0 41.0 32.0 41.0 20-21 37.64407625870088 40.0 38.0 41.0 32.0 41.0 22-23 37.57982314728595 40.0 37.5 41.0 32.0 41.0 24-25 37.48445037554973 40.0 37.0 41.0 32.0 41.0 26-27 37.35274833657127 40.0 37.0 41.0 31.0 41.0 28-29 37.210171092611105 40.0 37.0 41.0 31.0 41.0 30-31 37.00951988664313 40.0 37.0 41.0 30.5 41.0 32-33 36.79822351240545 40.0 36.0 41.0 30.0 41.0 34-35 36.46129259812484 39.0 36.0 41.0 30.0 41.0 36-37 36.191240316311706 39.0 35.0 41.0 29.0 41.0 38-39 35.94595708947836 39.0 35.0 41.0 28.0 41.0 40-41 35.78921289071032 39.0 35.0 41.0 27.0 41.0 42-43 35.57462088847649 39.0 35.0 40.0 27.0 41.0 44-45 35.500576622636515 39.0 35.0 41.0 26.0 41.0 46-47 35.28862055127982 39.0 35.0 41.0 25.5 41.0 48-49 35.04020355901697 39.0 35.0 41.0 24.0 41.0 50-51 33.94604424624501 38.0 33.0 40.0 23.0 40.5 52-53 34.059062078886875 38.0 33.5 39.5 23.0 40.5 54-55 34.536821590711014 38.0 34.0 40.0 23.0 41.0 56-57 34.413792976756696 38.0 34.0 40.0 23.0 41.0 58-59 34.179965251597295 38.0 34.0 40.0 22.0 41.0 60-61 33.841864967021266 38.0 33.5 40.0 21.5 41.0 62-63 33.45187089041557 37.0 33.0 40.0 19.5 41.0 64-65 33.04603693895638 37.0 32.5 39.5 18.0 41.0 66-67 32.60939439722829 36.0 32.0 39.0 15.5 41.0 68-69 32.152370847755705 35.5 31.5 39.0 12.5 40.5 70-71 31.688949134331235 35.0 31.0 38.5 8.0 40.0 72-73 31.234319232171337 35.0 31.0 37.5 7.0 40.0 74-75 30.747946509014298 35.0 30.0 37.0 4.5 39.0 76-77 30.293790764158686 35.0 30.0 36.5 2.0 39.0 78-79 29.865210529517157 34.0 29.5 36.0 2.0 38.5 80-81 29.392433445500895 34.0 29.0 35.5 2.0 37.0 82-83 28.904615389639734 34.0 29.0 35.0 2.0 37.0 84-85 28.462535653649216 34.0 28.5 35.0 2.0 36.5 86-87 27.99103509988737 34.0 27.0 35.0 2.0 36.0 88-89 27.482617838193768 33.5 26.0 35.0 2.0 36.0 90-91 26.99177787148888 33.0 25.5 35.0 2.0 35.0 92-93 26.446821717261816 33.0 25.0 35.0 2.0 35.0 94-95 25.865280336576163 32.5 23.5 35.0 2.0 35.0 96-97 25.16794476179668 32.0 19.0 35.0 2.0 35.0 98-99 24.234656428842317 32.0 2.0 34.0 2.0 35.0 100-101 20.9604979815146 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12449120460616925 1101 2 0.3191198528606911 1101 3 0.3695192101718483 1101 4 0.2953707755853543 1101 5 0.1769225188848509 1101 6 0.24245498923666275 1101 7 0.3266214745763989 1101 8 0.2566778103486911 1101 9 0.38469639410361367 1101 10-11 0.19513225103295895 1101 12-13 0.30406989445619814 1101 14-15 0.45053984549030446 1101 16-17 0.42268295039463766 1101 18-19 0.3915181248192354 1101 20-21 0.34665221241552757 1101 22-23 0.5063575496796346 1101 24-25 0.4840689706963275 1101 26-27 0.538950611823708 1101 28-29 0.5759715545834823 1101 30-31 0.4116870491455771 1101 32-33 0.45864050407578816 1101 34-35 0.4295658459041576 1101 36-37 0.45028181782968346 1101 38-39 0.5997543277594772 1101 40-41 0.48388646698195004 1101 42-43 0.44313746286933764 1101 44-45 0.53819412047676 1101 46-47 0.5455858329554459 1101 48-49 0.5684933466799933 1101 50-51 0.47734271157859354 1101 52-53 0.49893272296787927 1101 54-55 0.6259803166546476 1101 56-57 0.5868547236648141 1101 58-59 0.6395346892817315 1101 60-61 0.8024803230315101 1101 62-63 0.7674211040839651 1101 64-65 0.6552078659146261 1101 66-67 0.7180849488119634 1101 68-69 0.5365309507860658 1101 70-71 0.5341269618454341 1101 72-73 0.461680861340632 1101 74-75 0.4327748155590534 1101 76-77 0.27165011543762674 1101 78-79 0.3746833281084285 1101 80-81 0.29531317161486825 1101 82-83 0.19644907684730484 1101 84-85 0.3693814863901679 1101 86-87 0.34183385497368945 1101 88-89 0.16543275706143845 1101 90-91 0.20459359190072846 1101 92-93 0.2236711164590055 1101 94-95 0.07181785371246363 1101 96-97 -0.19310298905916667 1101 98-99 -0.05618334840671935 1101 100-101 0.3162016872103841 1102 1 0.2764953543751041 1102 2 0.32871807977759815 1102 3 0.2958438352779531 1102 4 0.06484450943426623 1102 5 0.2462066837438499 1102 6 0.32943037356185556 1102 7 0.35184924030308196 1102 8 0.4078095655123235 1102 9 0.47868300166751254 1102 10-11 0.3897130300577629 1102 12-13 0.3757892191756156 1102 14-15 0.4992284241944489 1102 16-17 0.5376306067183307 1102 18-19 0.5413978758331055 1102 20-21 0.5134210514233573 1102 22-23 0.5098612750403788 1102 24-25 0.5742698572378657 1102 26-27 0.6028994942154853 1102 28-29 0.506403979368919 1102 30-31 0.5550445894643588 1102 32-33 0.5192563675106712 1102 34-35 0.5034911500633541 1102 36-37 0.4208557120105709 1102 38-39 0.5392733318149325 1102 40-41 0.5482258049051794 1102 42-43 0.4173669262287589 1102 44-45 0.6241900650207555 1102 46-47 0.7844036203740998 1102 48-49 0.7639993355510626 1102 50-51 0.6706785599233953 1102 52-53 0.6727424937874531 1102 54-55 0.6728783643769916 1102 56-57 0.72491753607639 1102 58-59 0.7448091399089094 1102 60-61 0.881913030755733 1102 62-63 0.7915958659438118 1102 64-65 0.7814900502020947 1102 66-67 0.811387787631169 1102 68-69 0.894294319643933 1102 70-71 0.803504496505429 1102 72-73 0.6275046847868282 1102 74-75 0.535740014087775 1102 76-77 0.5754485687055713 1102 78-79 0.4268926085007685 1102 80-81 0.5052641288911133 1102 82-83 0.5190407961720247 1102 84-85 0.36943477319578477 1102 86-87 0.37602323533773685 1102 88-89 0.33638013044633297 1102 90-91 0.3373838399437332 1102 92-93 0.4011671081594663 1102 94-95 0.3566259268992944 1102 96-97 0.3039945305107672 1102 98-99 0.3216172740586174 1102 100-101 0.5479417607744708 1103 1 0.3518077842187033 1103 2 0.3547099977600858 1103 3 0.3191417474234335 1103 4 0.3032879727284339 1103 5 0.18211029820168534 1103 6 0.3063255318346947 1103 7 0.2825204967574706 1103 8 0.2532821390362372 1103 9 0.34104624345453516 1103 10-11 0.4370702851651487 1103 12-13 0.48030616896233624 1103 14-15 0.5967368429626916 1103 16-17 0.610530404667891 1103 18-19 0.6925297324521225 1103 20-21 0.7086317077131028 1103 22-23 0.5927936255604749 1103 24-25 0.5839589240641772 1103 26-27 0.700562818319348 1103 28-29 0.5715914372750817 1103 30-31 0.644929345881323 1103 32-33 0.5462555443422161 1103 34-35 0.5848123354259229 1103 36-37 0.5829772416072245 1103 38-39 0.8467439596679611 1103 40-41 0.7676102994049216 1103 42-43 0.7024466239014373 1103 44-45 0.8225725390161145 1103 46-47 0.7672865807871787 1103 48-49 0.830747071837834 1103 50-51 0.7076545383713508 1103 52-53 0.6611541528460521 1103 54-55 0.61230177229438 1103 56-57 0.48695412965567897 1103 58-59 0.6743028913120384 1103 60-61 0.662789330987728 1103 62-63 0.5543456227349566 1103 64-65 0.4725029215633114 1103 66-67 0.6511457012733146 1103 68-69 0.6640043398489794 1103 70-71 0.5984300371774403 1103 72-73 0.5781179452822265 1103 74-75 0.5437404630887457 1103 76-77 0.5879588583112003 1103 78-79 0.5458928330012576 1103 80-81 0.5498865939064537 1103 82-83 0.6341635231046006 1103 84-85 0.5857473527063775 1103 86-87 0.6031440165994333 1103 88-89 0.6922916024211894 1103 90-91 0.6247800084687647 1103 92-93 0.4956956571379898 1103 94-95 0.45808181255371494 1103 96-97 0.36764041841018624 1103 98-99 0.41452343285529736 1103 100-101 0.40948557578607137 1104 1 0.3583573216310647 1104 2 0.4666662568269011 1104 3 0.4306510860106805 1104 4 0.4502054592727873 1104 5 0.3833213252208765 1104 6 0.3606996251533161 1104 7 0.38651218577323476 1104 8 0.4574100658203548 1104 9 0.49950440290334797 1104 10-11 0.5501035690089822 1104 12-13 0.5207374148931265 1104 14-15 0.5887013272877724 1104 16-17 0.6189808783407784 1104 18-19 0.6394045777204624 1104 20-21 0.7219944268223841 1104 22-23 0.7849401482272356 1104 24-25 0.6752163354800729 1104 26-27 0.6634694346638952 1104 28-29 0.7635310352055384 1104 30-31 0.6958574033236289 1104 32-33 0.7230032639207664 1104 34-35 0.8326421198768585 1104 36-37 0.8964235989558986 1104 38-39 0.9967531234777098 1104 40-41 0.9293109879469839 1104 42-43 0.9377303029345683 1104 44-45 0.9450660707705651 1104 46-47 0.8776818513745255 1104 48-49 0.8041369202580739 1104 50-51 0.6939903003606105 1104 52-53 0.751934066957503 1104 54-55 0.919816964634613 1104 56-57 0.9240322895626818 1104 58-59 0.8326089980305227 1104 60-61 0.9156889562322448 1104 62-63 1.023200678608326 1104 64-65 0.9684086754751178 1104 66-67 1.062797984207421 1104 68-69 1.0478630279904948 1104 70-71 0.9597912028731521 1104 72-73 0.9379389073492277 1104 74-75 0.7773708689051055 1104 76-77 0.8261220243779057 1104 78-79 0.6627080359094357 1104 80-81 0.7462541040623947 1104 82-83 0.9767356456129122 1104 84-85 0.9381714020904397 1104 86-87 1.1046716569405923 1104 88-89 1.0049522924117191 1104 90-91 0.9691258639510529 1104 92-93 1.0641366603091136 1104 94-95 1.1547370774210748 1104 96-97 1.0910719289090345 1104 98-99 1.135521730160324 1104 100-101 1.0548404110628944 1105 1 0.19514673844225783 1105 2 0.3098501309890018 1105 3 0.32652034746545766 1105 4 0.25202861700025636 1105 5 0.25373520085411627 1105 6 0.28481326513254146 1105 7 0.15150703497988616 1105 8 0.3355634642283647 1105 9 0.2421116675627033 1105 10-11 0.38535455023557574 1105 12-13 0.32044710569496715 1105 14-15 0.4327121037741719 1105 16-17 0.4648430017882177 1105 18-19 0.5300603728482187 1105 20-21 0.4726144901919298 1105 22-23 0.4180912855104353 1105 24-25 0.47919812821011476 1105 26-27 0.47840049529922624 1105 28-29 0.42999235945011804 1105 30-31 0.4744005895378294 1105 32-33 0.56423221292129 1105 34-35 0.5682858819749654 1105 36-37 0.6242710607367243 1105 38-39 0.6910589345775549 1105 40-41 0.6852561680506071 1105 42-43 0.5889658130971114 1105 44-45 0.7455856124941675 1105 46-47 0.7933326444552122 1105 48-49 0.7918854875321131 1105 50-51 0.7564139431823449 1105 52-53 0.7151326960247388 1105 54-55 0.7460765054838774 1105 56-57 0.6204198229557676 1105 58-59 0.6856997370691431 1105 60-61 0.7415927758008323 1105 62-63 0.773477627851193 1105 64-65 0.7765564523622288 1105 66-67 0.7855556023296515 1105 68-69 0.7646208198919098 1105 70-71 0.7034352472263983 1105 72-73 0.7411082188879661 1105 74-75 0.6792186423275162 1105 76-77 0.5788187308995028 1105 78-79 0.6161667768665247 1105 80-81 0.8403062846193343 1105 82-83 0.8182421333994832 1105 84-85 0.8163101979675567 1105 86-87 0.8100294071567724 1105 88-89 0.749539605841715 1105 90-91 0.8768040379562585 1105 92-93 0.8699805024762526 1105 94-95 0.8451485477181642 1105 96-97 0.7438124067437535 1105 98-99 0.8797381755850431 1105 100-101 0.7117965760791094 1106 1 0.25510331930020413 1106 2 0.2631791685609386 1106 3 0.3110319974993665 1106 4 0.27106665046459 1106 5 0.29234113752536217 1106 6 0.3522655946067914 1106 7 0.3411769713955195 1106 8 0.3621208552456139 1106 9 0.4042182592488075 1106 10-11 0.3283100340767646 1106 12-13 0.41822254033017003 1106 14-15 0.45253313738407286 1106 16-17 0.466719024694207 1106 18-19 0.558387280290539 1106 20-21 0.4712215629323069 1106 22-23 0.6206943039041022 1106 24-25 0.6141401531823334 1106 26-27 0.674355467909308 1106 28-29 0.5917620356349076 1106 30-31 0.5548235445246377 1106 32-33 0.5360576097466918 1106 34-35 0.5793881175459958 1106 36-37 0.6820888139470043 1106 38-39 0.8266847311572789 1106 40-41 0.8077490054897609 1106 42-43 0.5944330570289083 1106 44-45 0.6514882016667158 1106 46-47 0.7179750489455259 1106 48-49 0.5743939281707213 1106 50-51 0.4754154981769645 1106 52-53 0.6411860451210316 1106 54-55 0.7198951710996866 1106 56-57 0.5983391655977641 1106 58-59 0.6550941234675491 1106 60-61 0.7714179631634863 1106 62-63 0.7272827493928418 1106 64-65 0.6935543541845561 1106 66-67 0.6340732096260524 1106 68-69 0.5265157299471852 1106 70-71 0.7080660602613698 1106 72-73 0.6291506475197473 1106 74-75 0.6677330721805781 1106 76-77 0.502302788654422 1106 78-79 0.6647168686396085 1106 80-81 0.7107299674004288 1106 82-83 0.7454346580646067 1106 84-85 0.7211300600061605 1106 86-87 0.8357272908729989 1106 88-89 0.8317418403476822 1106 90-91 0.984314786235295 1106 92-93 1.1013552703808784 1106 94-95 0.9321630112982753 1106 96-97 0.9233405297800381 1106 98-99 1.0368328385452834 1106 100-101 1.29076784773099 1107 1 0.13139587021887777 1107 2 0.19645304032543365 1107 3 0.19694238480434834 1107 4 0.18019814101037213 1107 5 0.09231664653839289 1107 6 0.17987162668477197 1107 7 0.13689678060899269 1107 8 0.21643422176625648 1107 9 0.2223740705605195 1107 10-11 0.13997886035055274 1107 12-13 0.44335828292815904 1107 14-15 0.288932128563836 1107 16-17 0.3289851010296658 1107 18-19 0.33211583330744787 1107 20-21 0.2761445987030413 1107 22-23 0.3070043632730375 1107 24-25 0.1578455352938377 1107 26-27 0.15181568308260296 1107 28-29 0.26217397056183955 1107 30-31 0.3981817104450016 1107 32-33 0.4450828858348004 1107 34-35 0.37861795416853994 1107 36-37 0.3005525863927332 1107 38-39 0.57888761727245 1107 40-41 0.39448800452054655 1107 42-43 0.5185020521341812 1107 44-45 0.1925441279644815 1107 46-47 0.4593498791587223 1107 48-49 0.5384668661512109 1107 50-51 0.5542208069891998 1107 52-53 0.6066283071850123 1107 54-55 0.620302919978478 1107 56-57 0.5209126472434207 1107 58-59 0.6247797529901362 1107 60-61 0.7465269567402473 1107 62-63 0.5910571057662324 1107 64-65 0.5737123123657568 1107 66-67 0.5701308902427371 1107 68-69 0.7074647817464879 1107 70-71 0.6641980996831727 1107 72-73 0.7014901620771212 1107 74-75 0.686104699459019 1107 76-77 0.7847171309932541 1107 78-79 0.7661034753699383 1107 80-81 0.7172940729930595 1107 82-83 0.7330304074019622 1107 84-85 0.780710812522134 1107 86-87 0.924805700244189 1107 88-89 0.7057468288664488 1107 90-91 1.0506371605756861 1107 92-93 1.0802689648375754 1107 94-95 1.0865200620970974 1107 96-97 1.0528689306401766 1107 98-99 0.8625993543289425 1107 100-101 0.9374296914680755 1108 1 0.2667905538469526 1108 2 0.20024783550710978 1108 3 0.2367643294777082 1108 4 0.21250743502136515 1108 5 0.2151003930714026 1108 6 0.20514479400889485 1108 7 0.4021095439907185 1108 8 0.3022309556849194 1108 9 0.3725488886806261 1108 10-11 0.4025075221826313 1108 12-13 0.37305037167962496 1108 14-15 0.49934678692198275 1108 16-17 0.5088000870540768 1108 18-19 0.5364205109363738 1108 20-21 0.4232248805283234 1108 22-23 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-2.1553875746876727 2316 68-69 -2.3738548349454405 2316 70-71 -2.09342787064433 2316 72-73 -2.325784868353267 2316 74-75 -2.256216507651356 2316 76-77 -2.2037633878161706 2316 78-79 -2.2455787079243485 2316 80-81 -2.361293842123377 2316 82-83 -2.1539015347458523 2316 84-85 -1.9381165553066282 2316 86-87 -2.0672255569327938 2316 88-89 -2.097268722210675 2316 90-91 -2.5014899523267964 2316 92-93 -2.4218808990869114 2316 94-95 -2.456041645564472 2316 96-97 -2.6333092134505875 2316 98-99 -2.5271085713520094 2316 100-101 -2.122252985602344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9952.0 3 3176.0 4 3286.0 5 3717.0 6 4220.0 7 4973.0 8 5929.0 9 7237.0 10 8764.0 11 10659.0 12 13007.0 13 16973.0 14 15234.0 15 15522.0 16 18568.0 17 23239.0 18 21276.0 19 17219.0 20 17754.0 21 19064.0 22 20540.0 23 22689.0 24 25708.0 25 29074.0 26 33222.0 27 38372.0 28 44527.0 29 52794.0 30 62527.0 31 74775.0 32 92077.0 33 117042.0 34 154625.0 35 217009.0 36 323629.0 37 465424.0 38 382476.0 39 53314.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36525072489884 18.935954982553284 14.806079321134282 22.892714971413593 2 15.15542522473418 20.079209069695892 40.841006975840536 23.924358729729388 3 19.78038008718436 24.079652570173273 29.553437603688742 26.586529738953622 4 12.968086201908225 16.775544196948868 37.19422890516581 33.06214069597711 5 14.08016910229389 36.85445865482427 34.15199343796783 14.913378804914004 6 31.81249119252677 35.73548783700711 17.633694890746483 14.818326079719633 7 28.313940521812725 32.418781794312295 20.85706886565328 18.410208818221697 8 25.559886754333615 35.148597153172894 19.741525058524285 19.549991033969206 9 25.752147826683704 17.73950432506446 20.75736829549651 35.75097955275533 10-11 25.013770785103244 26.42853131028229 27.173389349163184 21.38430855545128 12-13 26.119673215602667 23.805076346159776 27.57482818208053 22.500422256157027 14-15 22.91882598811741 25.27443082243776 26.2686700676639 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22.666482130774433 26.529741034631236 28.547825552069877 22.25595128252445 42-43 22.325950671683696 26.629226250077426 28.213115029807255 22.83170804843162 44-45 22.616625017255636 26.582559675780722 28.18380056362596 22.617014743337684 46-47 22.875580785513883 26.79416651676779 27.882114462305374 22.44813823541296 48-49 22.76303364883497 26.801849806889532 27.854166978653534 22.580949565621967 50-51 22.404998868686153 27.03809317307994 28.08368153115277 22.47322642708113 52-53 22.701265432250718 27.188639008003516 27.560198701253945 22.54989685849182 54-55 22.605837242139792 26.875878404599824 27.685241325895593 22.83304302736479 56-57 22.68560919419543 27.107143252642274 27.55769032794167 22.64955722522063 58-59 22.9998086966542 26.81521347546509 27.613458979785204 22.571518848095508 60-61 22.706299889445418 26.818606454797035 28.12449913282086 22.350594522936692 62-63 22.405316260569126 26.90488330224757 28.116797429240748 22.57300300794256 64-65 22.39238519281961 27.169671899771224 27.812431276525214 22.62551163088396 66-67 22.50389300244351 27.037768791558698 27.488905712404062 22.96943249359373 68-69 22.35703844509175 26.92890272138491 27.829786430748193 22.884272402775142 70-71 22.670132381383464 26.725802407478916 27.696882375125316 22.9071828360123 72-73 22.550217183110842 26.60796745659005 27.943112222079066 22.898703138220043 74-75 22.346482643616188 27.29561041341996 27.485940176095554 22.8719667668683 76-77 22.54029110262678 26.885724517854353 27.47788178760527 23.0961025919136 78-79 22.556816235946542 26.96405936397151 27.586807268065012 22.892317132016938 80-81 22.65925645184181 26.919227591954996 27.47394775851871 22.947568197684483 82-83 22.60617700433503 26.81732337469276 27.727584854640387 22.84891476633183 84-85 22.58223571537863 26.9337376357742 27.513589213788453 22.970437435058717 86-87 22.51709445359895 26.940916301272594 27.648568635631037 22.89342060949742 88-89 22.54107128608769 26.89500810523701 27.35996954408221 23.203951064593088 90-91 22.562374720772592 26.72988675404048 27.484367470553035 23.223371054633898 92-93 22.746772046670944 26.796279914063486 27.480419736511692 22.976528302753874 94-95 22.68170199809518 26.698799452270872 27.51575624002091 23.10374230961304 96-97 22.401024630186132 26.634476352099895 27.794694014209913 23.169805003504063 98-99 22.469698297023672 26.74259796058086 27.360049205710602 23.427654536684862 100-101 22.829861769905587 25.892498005754337 27.89743023832287 23.380209986017206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1952.0 1 1129.0 2 361.5 3 433.0 4 388.0 5 398.5 6 663.5 7 1355.0 8 2383.0 9 2932.0 10 2715.0 11 2430.5 12 2561.0 13 2835.0 14 3058.5 15 3328.5 16 3501.5 17 3662.0 18 3913.5 19 4119.5 20 4424.5 21 5049.0 22 5909.5 23 7133.0 24 8583.0 25 10378.0 26 13133.0 27 16766.5 28 20184.5 29 23544.0 30 27057.0 31 31047.5 32 35436.0 33 39603.0 34 44365.0 35 48751.5 36 53862.0 37 58786.0 38 62297.5 39 65262.5 40 68103.5 41 71893.5 42 74575.5 43 77160.0 44 81253.5 45 84668.5 46 87185.5 47 93929.5 48 106657.5 49 107269.5 50 96714.0 51 91439.5 52 89132.0 53 87348.5 54 84527.0 55 79113.0 56 72425.5 57 65061.0 58 58736.5 59 53117.0 60 45542.5 61 37150.0 62 29164.5 63 22286.0 64 16871.5 65 12452.5 66 8962.0 67 6807.0 68 4956.5 69 3384.0 70 2389.5 71 1550.0 72 1000.5 73 666.5 74 436.5 75 286.0 76 196.0 77 122.5 78 76.5 79 54.5 80 42.0 81 34.0 82 26.5 83 20.0 84 16.0 85 14.5 86 11.0 87 7.5 88 7.5 89 9.0 90 8.0 91 6.5 92 7.0 93 6.0 94 3.0 95 3.0 96 3.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32205139677393413 2 0.08825898337244842 3 0.0971991856659573 4 0.10275109211306784 5 0.11454889331317775 6 0.05731526949811174 7 0.05923394304968671 8 0.06041780545384998 9 0.06339787288501961 10-11 0.060887268131363005 12-13 0.05921353162892527 14-15 0.05737650376039605 16-17 0.05968299430643829 18-19 0.06772509408644399 20-21 0.06850072807537856 22-23 0.0847686304222429 24-25 0.09297402156834009 26-27 0.08795281206102688 28-29 0.08570755577726895 30-31 0.08048223206234137 32-33 0.07180737823873115 34-35 0.31874474661058155 36-37 0.49373185679837067 38-39 0.46862580926180464 40-41 0.4514598044014371 42-43 0.48089307313942753 44-45 0.48985368685369785 46-47 0.4839343748328815 48-49 0.5010391454309646 50-51 0.497120152644769 52-53 0.4972630325900991 54-55 0.4878737790398386 56-57 0.4679114095351544 58-59 0.45205173560351875 60-61 0.41190247096577454 62-63 0.47885193106328394 64-65 0.5023250649389351 66-67 0.5120817240629014 68-69 0.5239611709460571 70-71 0.5219608517114364 72-73 0.5100814048282808 74-75 0.5112244443909212 76-77 0.48628168822044665 78-79 0.4679726437974387 80-81 0.4744838870203367 82-83 0.4814237700792249 84-85 0.4829342152155711 86-87 0.4829342152155711 88-89 0.4893638127554234 90-91 0.5022638306766508 92-93 0.5086934282165031 94-95 0.5159190711660514 96-97 0.5077136800199542 98-99 0.5100201705659965 100-101 0.4886698244495346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2449609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.910840708108154 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32278199419312 50.53173743268782 2 11.807437598997545 14.856316519229228 3 3.4703132505749292 6.549609723424693 4 1.49641336874653 3.7656249229878576 5 0.7973589139021788 2.508125980984505 6 0.4650216169829725 1.75529405231056 7 0.3200376454810307 1.409368613481858 8 0.2183441941544568 1.0988973454393016 9 0.1652584763060831 0.9356894710700993 >10 0.86345633295992 9.788593764443036 >50 0.051474292690190486 2.2126033421627755 >100 0.019896461291944088 2.1700347810113834 >500 0.0011029268595935545 0.428193596029061 >1k 9.082927078929621E-4 1.136829304176235 >5k 1.9463415169134905E-4 0.8530811505614291 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7791 0.3180507583046927 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6731 0.27477854629044884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6379 0.26040890607439804 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3938 0.1607603499170684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3850 0.15716793986305572 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2800 0.1143039562640405 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2524 0.10303685200372793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.082284152287161E-5 6 0.0 0.0 0.0 0.0 4.082284152287161E-5 7 4.082284152287161E-5 0.0 0.0 4.082284152287161E-5 4.082284152287161E-5 8 4.082284152287161E-5 0.0 0.0 4.082284152287161E-5 4.082284152287161E-5 9 4.082284152287161E-5 0.0 0.0 1.2246852456861482E-4 4.082284152287161E-5 10-11 4.082284152287161E-5 0.0 0.0 1.6329136609148645E-4 8.164568304574321E-5 12-13 4.082284152287161E-5 0.0 0.0 2.4493704913722963E-4 2.8575989066010124E-4 14-15 4.082284152287161E-5 0.0 0.0 2.4493704913722963E-4 2.8575989066010124E-4 16-17 4.082284152287161E-5 0.0 0.0 2.6534846989866544E-4 3.0617131142153704E-4 18-19 4.082284152287161E-5 0.0 0.0 2.8575989066010124E-4 3.674055737058445E-4 20-21 4.082284152287161E-5 0.0 0.0 3.265827321829729E-4 5.306969397973309E-4 22-23 4.082284152287161E-5 0.0 0.0 4.6946267751302347E-4 5.715197813202025E-4 24-25 6.123426228430742E-5 0.0 0.0 5.715197813202025E-4 5.715197813202025E-4 26-27 1.0205710380717901E-4 0.0 0.0 9.185139342646111E-4 5.919312020816383E-4 28-29 1.2246852456861482E-4 0.0 0.0 0.001857439289290658 6.123426228430741E-4 30-31 1.6329136609148642E-4 0.0 0.0 0.005694786392440589 6.123426228430741E-4 32-33 2.0411420761435806E-4 0.0 0.0 0.0132266006534104 6.123426228430741E-4 34-35 2.0411420761435806E-4 0.0 0.0 0.024452882072200095 6.123426228430741E-4 36-37 2.0411420761435806E-4 0.0 0.0 0.043823320374802674 6.123426228430741E-4 38-39 2.0411420761435806E-4 0.0 0.0 0.07095009856675086 6.531654643659458E-4 40-41 2.0411420761435806E-4 0.0 0.0 0.1026082121677378 6.531654643659458E-4 42-43 2.8575989066010124E-4 0.0 0.0 0.13706269041304142 6.939883058888174E-4 44-45 2.8575989066010124E-4 0.0 0.0 0.17931433138921354 8.164568304574322E-4 46-47 3.469941529444087E-4 0.0 0.0 0.2328738994672211 9.185139342646112E-4 48-49 3.674055737058445E-4 0.0 0.0 0.2883521410968036 9.797481965489185E-4 50-51 3.674055737058445E-4 0.0 0.0 0.3498517518510097 0.0010205710380717901 52-53 3.674055737058445E-4 0.0 0.0 0.4135762074682123 0.0010205710380717901 54-55 3.674055737058445E-4 0.0 0.0 0.4856489341768421 0.0011634509834018408 56-57 3.674055737058445E-4 0.0 0.0 0.5762960537783786 0.0011838624041632766 58-59 3.674055737058445E-4 0.0 0.0 0.6795982542520052 0.0011838624041632766 60-61 3.674055737058445E-4 0.0 0.0 0.7882686583858893 0.0012246852456861482 62-63 3.674055737058445E-4 0.0 0.0 0.9030624887482043 0.0013063309287318916 64-65 3.674055737058445E-4 0.0 0.0 1.0256943046829106 0.0013063309287318916 66-67 4.082284152287161E-4 0.0 0.0 1.1698397581001703 0.0013267423494933274 68-69 4.082284152287161E-4 0.0 0.0 1.3419284465398356 0.0013471537702547632 70-71 4.082284152287161E-4 0.0 0.0 1.530040100277228 0.0013471537702547632 72-73 4.082284152287161E-4 0.0 0.0 1.7213155242326428 0.0014287994533005064 74-75 4.082284152287161E-4 0.0 0.0 1.9243275151258834 0.0015512679778691212 76-77 4.082284152287161E-4 0.0 0.0 2.149547131807566 0.0015920908193919928 78-79 4.082284152287161E-4 0.0 0.0 2.417589909246741 0.0016329136609148644 80-81 4.490512567515877E-4 0.0 0.0 2.7170458632377654 0.0016329136609148644 82-83 4.898740982744593E-4 0.0 0.0 3.0195635303430057 0.001673736502437736 84-85 5.102855190358951E-4 0.0 0.0 3.3269391155894676 0.001673736502437736 86-87 5.306969397973309E-4 0.0 0.0 3.6568080865150314 0.0017962050270063509 88-89 5.511083605587667E-4 0.0 0.0 4.021213181368945 0.0019390849723364013 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7990 0.0 22.038235 1 GTATCAA 17385 0.0 21.949825 1 GAGTACT 7135 0.0 21.628555 12-13 TACATGG 7890 0.0 21.36884 2 GTACTTT 7280 0.0 21.002907 14-15 AGTACTT 7525 0.0 19.878538 12-13 GTATAGG 550 0.0 19.847912 1 ACATGGG 8260 0.0 19.723606 3 AGAGTAC 11915 0.0 19.685013 10-11 TCAACGC 19285 0.0 19.37605 4 CAACGCA 19300 0.0 19.336454 5 TATCAAC 19395 0.0 19.314997 2 ATCAACG 19420 0.0 19.241356 3 AACGCAG 19680 0.0 19.050003 6 GGTATCA 13445 0.0 18.285997 1 ACGCAGA 20935 0.0 17.546227 7 CGCAGAG 20880 0.0 17.388409 8 ACTTTTT 9060 0.0 17.24384 16-17 TACTTTT 8745 0.0 17.18669 14-15 GTGTAGC 1515 0.0 16.91729 1 >>END_MODULE