FastQCFastQC Report
Thu 26 May 2016
SRR938893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449609
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77460.3162137304361635No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76450.31209062344235344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.28228994913065714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.17247650543413257No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35300.14410463057573678No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24930.10177134391651892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85400.021.813791
TACATGG83950.021.199972
GTAAGCG3650.020.15451694-95
GAGTACT77400.020.05160912-13
TTTGCGT4500.020.0385273
ACATGGG84500.019.770183
GTACTTT80650.019.30242514-15
AGAGTAC125300.018.6361610-11
GTGTAGC14650.018.5374261
GTATCAA187850.018.362831
AGTACTT81900.018.34138912-13
CATGGGG49300.017.6168754
CCGTGCG1901.878636E-417.4854979
GGTATCA153400.017.4551331
CTTTGCG5300.016.1183782
GGACCGT2658.008286E-616.1183786
ACTTTTT96300.016.11575116-17
ATCAACG211050.016.0110323
TCAACGC211650.015.9880674
TATCAAC212250.015.987592
Produced by FastQC (version 0.11.5)