##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938893_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2449609 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18028795615953 33.0 31.0 34.0 30.0 34.0 2 32.42244905207321 34.0 31.0 34.0 31.0 34.0 3 32.513394994874695 34.0 31.0 34.0 31.0 34.0 4 36.05690908222496 37.0 35.0 37.0 35.0 37.0 5 35.980102130584925 37.0 35.0 37.0 35.0 37.0 6 36.01887280786444 37.0 35.0 37.0 35.0 37.0 7 35.96218702658261 37.0 35.0 37.0 35.0 37.0 8 35.92930300305069 37.0 35.0 37.0 35.0 37.0 9 37.6086065163869 39.0 37.0 39.0 35.0 39.0 10-11 37.50967235995623 39.0 37.0 39.0 35.0 39.0 12-13 37.41834921409907 39.0 37.0 39.0 34.0 39.0 14-15 38.73178862422533 40.0 38.0 41.0 34.5 41.0 16-17 38.83476158848208 40.0 38.0 41.0 35.0 41.0 18-19 38.86476188648882 40.0 38.0 41.0 35.0 41.0 20-21 38.83404780109805 40.0 38.0 41.0 34.0 41.0 22-23 38.757366175581495 40.0 38.0 41.0 34.0 41.0 24-25 38.67923533919087 40.0 38.0 41.0 34.0 41.0 26-27 38.51424921283356 40.0 38.0 41.0 34.0 41.0 28-29 38.35831657215499 40.0 38.0 41.0 34.0 41.0 30-31 38.17207337987409 40.0 38.0 41.0 33.0 41.0 32-33 38.078372099384026 40.0 37.5 41.0 33.0 41.0 34-35 37.97942794135717 40.0 37.0 41.0 33.0 41.0 36-37 37.85120686607536 40.0 37.0 41.0 33.0 41.0 38-39 37.71068995092686 40.0 37.0 41.0 33.0 41.0 40-41 37.542976450527405 40.0 37.0 41.0 32.0 41.0 42-43 37.351672042354515 40.0 37.0 41.0 31.5 41.0 44-45 37.09931727879837 40.0 36.0 41.0 31.0 41.0 46-47 36.79665448649152 39.5 36.0 41.0 30.5 41.0 48-49 36.46312370668135 39.0 36.0 41.0 30.0 41.0 50-51 36.18882319586513 39.0 35.0 40.5 29.5 41.0 52-53 35.91601639282024 39.0 35.0 40.0 28.5 41.0 54-55 35.59784316599098 38.5 35.0 40.0 28.0 41.0 56-57 35.42582408049611 38.0 35.0 40.0 28.0 41.0 58-59 35.11665269845106 38.0 34.0 40.0 27.0 41.0 60-61 34.72206033697623 38.0 34.0 40.0 26.0 41.0 62-63 34.28127958380297 37.0 33.5 40.0 26.0 41.0 64-65 33.84728134163453 36.5 33.0 39.0 25.5 41.0 66-67 33.35703453081696 36.0 32.5 39.0 23.5 40.0 68-69 32.8354402682224 35.5 32.0 38.5 22.5 40.0 70-71 32.27745489178069 35.0 31.0 38.0 21.5 40.0 72-73 31.72733913861355 35.0 31.0 37.0 20.0 39.0 74-75 31.170241454860758 34.5 30.5 36.5 18.0 39.0 76-77 29.293108614476843 32.5 28.5 35.0 13.0 37.0 78-79 30.237895108974534 34.0 29.5 35.5 15.0 37.5 80-81 30.195820639130574 34.0 30.0 35.0 12.0 37.0 82-83 29.739239201031676 34.0 29.5 35.0 7.5 37.0 84-85 29.24045470113802 34.0 29.0 35.0 4.0 36.0 86-87 28.49543641454616 33.5 28.5 35.0 2.0 36.0 88-89 27.74915343632392 33.0 26.5 35.0 2.0 35.0 90-91 27.104340937676177 32.5 25.5 34.5 2.0 35.0 92-93 26.632923254282623 32.0 25.0 34.0 2.0 35.0 94-95 25.981837101349644 32.0 24.5 34.0 2.0 35.0 96-97 25.021456485504423 32.0 19.0 34.0 2.0 35.0 98-99 23.67056089359567 31.0 2.0 34.0 2.0 35.0 100-101 17.972485200699378 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1323512210400679 1101 2 0.1706099712447937 1101 3 0.17942656921096756 1101 4 0.07592782100071815 1101 5 0.1290686771931604 1101 6 0.12168339653806015 1101 7 0.17343652150717048 1101 8 0.12792473952246297 1101 9 0.24800426366857664 1101 10-11 0.22734322405693774 1101 12-13 0.27986915510012267 1101 14-15 0.3273306909055904 1101 16-17 0.28284732263858103 1101 18-19 0.3056640673743587 1101 20-21 0.2746569299107193 1101 22-23 0.2789620666051533 1101 24-25 0.20027804689463835 1101 26-27 0.24851487340464473 1101 28-29 0.20153208322459903 1101 30-31 0.27664155272172763 1101 32-33 0.21539044430146248 1101 34-35 0.3105057150800121 1101 36-37 0.42862592890911344 1101 38-39 0.3745680355429144 1101 40-41 0.3785192212369992 1101 42-43 0.4045441445513589 1101 44-45 0.40200531243298343 1101 46-47 0.37457699595955773 1101 48-49 0.3504740900805814 1101 50-51 0.17588258558416214 1101 52-53 0.2681050047077207 1101 54-55 0.39454565615215387 1101 56-57 0.28818497829481515 1101 58-59 0.3401483600565314 1101 60-61 0.46228894551757804 1101 62-63 0.4123387547126214 1101 64-65 0.42984445688733075 1101 66-67 0.3454832045755154 1101 68-69 0.5184220511308553 1101 70-71 0.6135991201119779 1101 72-73 0.570163234224875 1101 74-75 0.35520906030359356 1101 76-77 0.29151666406030685 1101 78-79 0.6409942291656101 1101 80-81 0.7713854690416184 1101 82-83 0.7137864294072678 1101 84-85 0.8288975213137739 1101 86-87 1.0160348468219773 1101 88-89 0.9274504440362641 1101 90-91 0.98465140560576 1101 92-93 1.010493916466043 1101 94-95 0.778673810729277 1101 96-97 0.9279261972010104 1101 98-99 1.244765903460447 1101 100-101 1.3125353009633507 1102 1 0.11436102958481342 1102 2 0.1571364260217436 1102 3 0.15269734163386772 1102 4 0.0684091941969811 1102 5 0.1254065061095062 1102 6 0.09459955234301987 1102 7 0.08114094478359846 1102 8 0.1467571454453136 1102 9 0.15074876994038533 1102 10-11 0.24572434260829112 1102 12-13 0.2500983355207609 1102 14-15 0.28401229045288545 1102 16-17 0.2809624787264866 1102 18-19 0.22910271681320182 1102 20-21 0.17990968856393152 1102 22-23 0.1897160984829256 1102 24-25 0.26419910697197224 1102 26-27 0.25934058310944863 1102 28-29 0.19191263589837604 1102 30-31 0.19002738691959564 1102 32-33 0.28585965112971223 1102 34-35 0.20052787861862953 1102 36-37 0.21730932040208728 1102 38-39 0.29369233998505706 1102 40-41 0.4218654440041405 1102 42-43 0.3803547641873095 1102 44-45 0.5081045296356663 1102 46-47 0.5495255954125469 1102 48-49 0.6113752501296261 1102 50-51 0.5023519810382808 1102 52-53 0.7022321385377666 1102 54-55 0.7349978866529554 1102 56-57 0.5660733117853383 1102 58-59 0.2998456155502609 1102 60-61 0.4418654804605211 1102 62-63 0.5984389150446034 1102 64-65 0.5598161630082359 1102 66-67 0.5353077825251518 1102 68-69 0.4868069421994221 1102 70-71 0.6086104376636214 1102 72-73 0.7454079762790151 1102 74-75 0.8403029016692436 1102 76-77 0.7547784767548258 1102 78-79 0.9083614837162095 1102 80-81 0.9424200818870396 1102 82-83 0.7965342366316399 1102 84-85 1.0271399998575852 1102 86-87 1.1606943771435851 1102 88-89 0.8574627047172036 1102 90-91 0.9342609500743073 1102 92-93 1.0435006126840953 1102 94-95 1.0143349443706988 1102 96-97 1.1178686663135267 1102 98-99 1.2314126072235325 1102 100-101 1.380250098662117 1103 1 0.1525036622271898 1103 2 0.1355664192867323 1103 3 0.12332220132106642 1103 4 0.13286103884264833 1103 5 0.10316823625991844 1103 6 0.12999355069668184 1103 7 0.10814535995983476 1103 8 0.10632610451138902 1103 9 0.18286356452244235 1103 10-11 0.1503849727063269 1103 12-13 0.16997111857845226 1103 14-15 0.2094747614549064 1103 16-17 0.10942801790181989 1103 18-19 0.21897998988062284 1103 20-21 0.23196798385690443 1103 22-23 0.27233976083418554 1103 24-25 0.18794153008055048 1103 26-27 0.17157136960948094 1103 28-29 0.19704583952032095 1103 30-31 0.21201496455934432 1103 32-33 0.21625065614355776 1103 34-35 0.10551702776178473 1103 36-37 0.13792332923243578 1103 38-39 0.1483002124687829 1103 40-41 0.2107713783003291 1103 42-43 0.18480024798586925 1103 44-45 0.10742391899434267 1103 46-47 0.06698200490143336 1103 48-49 0.03378900452609912 1103 50-51 -0.03545240420455542 1103 52-53 0.046602788840843345 1103 54-55 0.0528277152842449 1103 56-57 0.12280458470309696 1103 58-59 0.020977098001807803 1103 60-61 -0.02131643976697717 1103 62-63 0.23401887463451487 1103 64-65 0.05766170667357784 1103 66-67 0.12402588474770937 1103 68-69 0.22182826974562886 1103 70-71 0.15826395857957465 1103 72-73 0.047599101026449375 1103 74-75 0.034073013171155964 1103 76-77 -0.08365899985970415 1103 78-79 0.15954422711215344 1103 80-81 -0.03908893554916659 1103 82-83 -0.08023931721105981 1103 84-85 0.11816634124798853 1103 86-87 0.31494866136120336 1103 88-89 0.18738712288677561 1103 90-91 0.2624967504185385 1103 92-93 0.2949265649403223 1103 94-95 0.512202938084684 1103 96-97 0.036145999247747085 1103 98-99 0.34020816211390326 1103 100-101 0.7791328345747139 1104 1 0.09680451157932879 1104 2 0.10601054628235573 1104 3 0.07598761362968531 1104 4 0.07962281452292785 1104 5 0.13045569684260272 1104 6 0.13482450427238035 1104 7 0.18139837939819614 1104 8 0.06244180204359395 1104 9 0.18888045549305588 1104 10-11 0.1772669901338233 1104 12-13 0.1841498971165194 1104 14-15 0.2724724431588399 1104 16-17 0.23231433714678218 1104 18-19 0.21254998845432738 1104 20-21 0.2919216548864796 1104 22-23 0.2048424822639845 1104 24-25 0.1778699304267093 1104 26-27 0.21128179691645244 1104 28-29 0.3772292487383666 1104 30-31 0.2530350222174462 1104 32-33 0.27396492116537274 1104 34-35 0.2495590918655921 1104 36-37 0.2512688477230114 1104 38-39 0.2565636040095498 1104 40-41 0.2947741377894886 1104 42-43 0.17760157659046882 1104 44-45 0.2714190703043542 1104 46-47 0.32595847386383525 1104 48-49 0.10184858259177076 1104 50-51 0.08785247574584076 1104 52-53 0.17138385507966802 1104 54-55 0.28690605772842304 1104 56-57 0.19329996252179882 1104 58-59 0.24716523397204782 1104 60-61 0.13278059611008075 1104 62-63 0.18544061385170352 1104 64-65 0.08661232482428005 1104 66-67 0.0269931485154018 1104 68-69 0.08236829527128009 1104 70-71 0.20946646842882188 1104 72-73 0.362562567725238 1104 74-75 0.27554568173906446 1104 76-77 0.16274448194458913 1104 78-79 0.3325637911069528 1104 80-81 0.3633038349439559 1104 82-83 0.3270814952050145 1104 84-85 0.4868206801271526 1104 86-87 0.6203537756538644 1104 88-89 0.5084142233760787 1104 90-91 0.8039346297651626 1104 92-93 0.9796249056256414 1104 94-95 1.0329464962589903 1104 96-97 0.832234973928287 1104 98-99 0.8748734133168341 1104 100-101 1.1794861955127907 1105 1 -0.018523193702776553 1105 2 0.00866046121581121 1105 3 0.08872845788815908 1105 4 0.07856036702628444 1105 5 0.06901169337645285 1105 6 0.008557598668403443 1105 7 0.020529824488235704 1105 8 0.02910757986055046 1105 9 0.028539405674486318 1105 10-11 0.054622691279327285 1105 12-13 0.06367303106272715 1105 14-15 0.021433259942789107 1105 16-17 0.045735260269800904 1105 18-19 0.06980843493006006 1105 20-21 0.09417164187117777 1105 22-23 0.10747706907493892 1105 24-25 0.03740156467847555 1105 26-27 -0.07250297229916214 1105 28-29 -0.005968281057597835 1105 30-31 0.06609487282176474 1105 32-33 0.19317592011816487 1105 34-35 0.26013304751707267 1105 36-37 0.13680069638709824 1105 38-39 0.17050163812432828 1105 40-41 0.2694215138779441 1105 42-43 0.1650346929174944 1105 44-45 0.10633582590134694 1105 46-47 0.18512450064940822 1105 48-49 0.11325352202029393 1105 50-51 0.12653439870143757 1105 52-53 0.14926011377701798 1105 54-55 0.14739837525528543 1105 56-57 0.1740424894271868 1105 58-59 0.20957559846754492 1105 60-61 0.2560266824957509 1105 62-63 0.1796490324193769 1105 64-65 0.08645913429825924 1105 66-67 0.15955292864664017 1105 68-69 0.18122851785012983 1105 70-71 0.35245726266683164 1105 72-73 0.4943795234585302 1105 74-75 0.4573736755345301 1105 76-77 0.1357385068791821 1105 78-79 0.35873225207093995 1105 80-81 0.5128273117321953 1105 82-83 0.49208492453230335 1105 84-85 0.5550591233123328 1105 86-87 0.5915778105852922 1105 88-89 0.8610162764737836 1105 90-91 0.5315613303024413 1105 92-93 0.6499738589506912 1105 94-95 0.7428244100307673 1105 96-97 0.8134947569437472 1105 98-99 0.8599479941561761 1105 100-101 0.8758534123411472 1106 1 -0.018211129713684215 1106 2 -0.0013071253118326354 1106 3 -0.015464879776438067 1106 4 -0.0045794795333762295 1106 5 0.02375140928816677 1106 6 -0.0033617191220898235 1106 7 0.12267912527318714 1106 8 -0.003049211880224334 1106 9 0.0056994458629304745 1106 10-11 0.12980179967807004 1106 12-13 0.08558061538922601 1106 14-15 0.012198260493072155 1106 16-17 -0.09718330132466946 1106 18-19 -0.09271131314660863 1106 20-21 -0.036570033759780074 1106 22-23 -0.01747242910202118 1106 24-25 0.010245873504672431 1106 26-27 0.029402694910686478 1106 28-29 -0.08318601225654021 1106 30-31 0.041006561198003055 1106 32-33 0.11678219405993673 1106 34-35 0.06837771420504168 1106 36-37 0.02880365726726808 1106 38-39 0.08957286610854709 1106 40-41 0.2201391012931424 1106 42-43 0.1902725573915518 1106 44-45 0.11464271012525273 1106 46-47 0.20116242405704554 1106 48-49 0.3485455095377432 1106 50-51 0.299540508623231 1106 52-53 0.2839091571713084 1106 54-55 0.353956600573305 1106 56-57 0.35408938778095234 1106 58-59 0.29852848000039245 1106 60-61 0.46674673731108385 1106 62-63 0.49302970788347267 1106 64-65 0.5460109017733075 1106 66-67 0.5009672637089295 1106 68-69 0.4113721595907265 1106 70-71 0.4978888446785916 1106 72-73 0.5428760069695464 1106 74-75 0.6088907715998246 1106 76-77 0.477007194328813 1106 78-79 0.717813438411131 1106 80-81 0.8133207030050507 1106 82-83 0.7535766188019011 1106 84-85 0.7776935256157138 1106 86-87 0.8718105627482622 1106 88-89 0.8568945644176047 1106 90-91 0.9682525467129643 1106 92-93 1.1835060931371437 1106 94-95 1.130224604582608 1106 96-97 1.0466172962002602 1106 98-99 1.2284586430291604 1106 100-101 1.2967115528089934 1107 1 0.004614817531688686 1107 2 0.016146437345142317 1107 3 -0.0096264716558494 1107 4 -0.007628694258542623 1107 5 0.03764422561270919 1107 6 0.023658092523113794 1107 7 0.030228602277709626 1107 8 0.033600073712470646 1107 9 -0.0799502253325457 1107 10-11 0.06731599923966769 1107 12-13 0.08850155639895263 1107 14-15 0.07665558538251815 1107 16-17 0.01800284754579451 1107 18-19 0.015162712858995064 1107 20-21 0.028995638561234216 1107 22-23 0.03719214859346209 1107 24-25 6.003327749510845E-4 1107 26-27 0.025530079308019538 1107 28-29 0.044625848328948337 1107 30-31 0.09385811107797792 1107 32-33 0.16185377374596044 1107 34-35 0.18654446829815896 1107 36-37 0.20702317988408936 1107 38-39 0.22674723151407505 1107 40-41 0.1593890344205846 1107 42-43 0.20379635880959768 1107 44-45 0.24671678407066366 1107 46-47 0.19098685320861364 1107 48-49 0.15520462626421505 1107 50-51 -0.09007652876635319 1107 52-53 -0.15443608584528334 1107 54-55 -0.021500217126906307 1107 56-57 0.09965078720637166 1107 58-59 0.054276479903116126 1107 60-61 0.16613619958627623 1107 62-63 0.2748884000995133 1107 64-65 0.2590233452790258 1107 66-67 0.10612774046680329 1107 68-69 0.04624194049192454 1107 70-71 0.16043934648328673 1107 72-73 0.1527865193348923 1107 74-75 0.2682222718725278 1107 76-77 0.06355078654867086 1107 78-79 0.42320223717164396 1107 80-81 0.29732383298792797 1107 82-83 0.2003945274453045 1107 84-85 0.403335067887852 1107 86-87 0.47575135361607934 1107 88-89 0.6372714650745159 1107 90-91 0.6490893915790608 1107 92-93 0.8660659918382514 1107 94-95 0.9708317899950138 1107 96-97 1.0862630801029098 1107 98-99 1.2833549297786675 1107 100-101 1.1921285248892666 1108 1 -0.005175893927393815 1108 2 -0.04804512636908953 1108 3 -0.05311139174347801 1108 4 -0.01734766706869806 1108 5 -0.13166884992180883 1108 6 -0.008902286759116862 1108 7 0.05065994883905489 1108 8 -0.019146843991642015 1108 9 -0.01880701615788638 1108 10-11 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#Quality Count 2 2.0 3 7.0 4 27.0 5 45.0 6 103.0 7 284.0 8 671.0 9 1302.0 10 2251.0 11 3388.0 12 4997.0 13 6667.0 14 8419.0 15 11475.0 16 14791.0 17 20647.0 18 21220.0 19 16483.0 20 16294.0 21 17475.0 22 19058.0 23 20859.0 24 23891.0 25 27949.0 26 33162.0 27 39531.0 28 47915.0 29 57341.0 30 69162.0 31 84247.0 32 102281.0 33 130028.0 34 173149.0 35 245637.0 36 375370.0 37 524765.0 38 314003.0 39 14713.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.18097113097531 19.690966524652026 15.443313536525194 22.68474880784747 2 15.65127332566136 20.71812276979714 39.9378839643388 23.692719940202704 3 19.843656681535705 24.29816350282841 29.82349428010756 26.034685535528325 4 13.507625094453848 17.418085906771243 36.709776948076204 32.3645120506987 5 14.036893234797878 37.0785296755523 34.13365153377539 14.750925555874428 6 31.72816559704018 36.26676747187 17.26038726996839 14.74467966112143 7 28.27238959360453 32.60883675721309 20.707549653842715 18.411223995339665 8 25.630171998878186 35.57661651308433 19.394033905002797 19.399177583034678 9 25.56367159003743 17.7819398932646 20.60283906533655 36.05154945136142 10-11 25.02617764712654 26.712569230436365 26.964160402741825 21.297092719695264 12-13 26.068405202626217 23.934472807701148 27.51088030783688 22.486241681835754 14-15 22.7257594817727 25.47122845269692 26.188677535325933 25.614334530204445 16-17 22.13860841283245 28.55922227522504 26.800209262455983 22.50196004948653 18-19 22.04611165711918 27.45855186413278 28.711349517624836 21.783986961123205 20-21 22.961501202845024 26.355063195799822 28.705132125167733 21.978303476187424 22-23 22.47056571069097 26.303667238322525 28.754711466197257 22.471055584789244 24-25 22.653082718983086 26.43128060950185 28.741283805932948 22.17435286558211 26-27 22.411928273436345 26.481333649846494 28.97930424392306 22.127433832794097 28-29 22.07164490332947 27.03958060245533 28.771714179691532 22.117060314523666 30-31 22.44837849632329 26.529458374785523 28.59023623770161 22.431926891189573 32-33 22.321623573394774 26.61908492334899 28.666432071404046 22.392859431852187 34-35 22.796290225054943 26.607318052041535 28.504666410540423 22.0917253123631 36-37 22.642298349507783 26.35795767053609 28.73351335040406 22.266230629552062 38-39 22.321434403874985 26.730233139213965 28.750828790635236 22.197503666275814 40-41 22.58238220804771 26.644388350608523 28.567786379649124 22.205443061694645 42-43 22.328687039162208 26.734950575203953 28.242903899155696 22.693458486478143 44-45 22.619276036202272 26.63344995244063 28.18596733304213 22.561306678314967 46-47 22.869855844440018 26.902533336381477 27.886254600796217 22.341356218382284 48-49 22.75457009928205 26.939708797970557 27.845798582711463 22.45992252003593 50-51 22.414796438342016 27.098622034426583 28.02255235009784 22.464029177133558 52-53 22.77792088451667 27.26428993361798 27.45640222582461 22.50138695604074 54-55 22.62836640459763 26.941115908702162 27.60177236448756 22.828745322212647 56-57 22.70370495862809 27.144821887901294 27.50687966936764 22.644593484102973 58-59 22.974587144690105 26.958442382328123 27.575138176353807 22.491832296627962 60-61 22.67746256184241 26.968434906818455 28.039090894617143 22.315011636721994 62-63 22.429047722272774 26.999055752256822 28.027134569387513 22.544761956082887 64-65 22.39060201144029 27.25117448321922 27.743542995801747 22.614680509538747 66-67 22.531689184210308 27.136666170654056 27.396613519834073 22.935031125301563 68-69 22.353863557406424 27.053352400835585 27.707311118849514 22.885472922908477 70-71 22.644786431135103 26.863951293431438 27.5921494377876 22.89911283764586 72-73 22.57944022903247 26.712814167485504 27.73671226714141 22.971033336340614 74-75 22.311887325691572 27.45654510576994 27.259554484001324 22.972013084537167 76-77 22.478100618762095 26.915017305194745 27.39287399027493 23.214008085768224 78-79 22.482274642762736 27.003922184992817 27.535392029687355 22.978411142557086 80-81 22.551144001701502 27.02382794273194 27.403726236455455 23.0213018191111 82-83 22.566336096903626 26.880820571772883 27.638819093169566 22.914024238153928 84-85 22.390741523936114 26.984892251972152 27.475481751336133 23.148884472755597 86-87 22.29585557520504 26.999827567608207 27.542580994191233 23.16173586299552 88-89 22.32027010044714 26.958977541309366 27.32058888588896 23.40016347235454 90-91 22.390569044452263 26.75102540454225 27.425844763884943 23.432560787120547 92-93 22.473749892329757 26.940013691981047 27.354916641798756 23.231319773890448 94-95 22.38708511978086 26.714402789868863 27.442099709031453 23.45641238131882 96-97 22.043252617050314 26.6812172881468 27.743080630418977 23.53244946438391 98-99 22.03345513508482 26.82387270784848 27.300948845305516 23.841723311761186 100-101 22.312747298280637 25.79103301664865 28.223861267004068 23.67235841806664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 440.0 2 267.5 3 310.5 4 302.5 5 302.0 6 531.0 7 1158.0 8 1976.5 9 2451.5 10 2311.0 11 2038.5 12 2073.5 13 2227.0 14 2416.5 15 2597.0 16 2736.0 17 2895.5 18 3117.0 19 3461.0 20 4018.5 21 4705.0 22 5598.0 23 6895.5 24 8356.0 25 10288.0 26 13307.5 27 17100.0 28 20566.5 29 23793.0 30 27274.5 31 31397.0 32 35540.0 33 39755.5 34 44630.5 35 49174.0 36 54388.5 37 59049.0 38 62653.5 39 65899.0 40 69041.5 41 72851.0 42 75424.5 43 77601.0 44 81553.0 45 85406.5 46 87862.0 47 94938.0 48 108088.0 49 108399.0 50 97598.5 51 92221.0 52 89651.0 53 88040.0 54 85567.0 55 79686.5 56 72320.0 57 64855.5 58 58567.5 59 52689.5 60 45015.5 61 36735.5 62 28772.0 63 21811.5 64 16443.5 65 12011.5 66 8459.0 67 6404.0 68 4577.0 69 3106.5 70 2191.0 71 1288.0 72 792.5 73 533.0 74 330.5 75 194.0 76 114.0 77 59.0 78 29.0 79 17.0 80 14.5 81 11.0 82 8.5 83 6.0 84 3.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3872046518444372 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011226281418789692 16-17 0.0021840220214736313 18-19 4.6946267751302347E-4 20-21 0.0 22-23 0.0 24-25 8.164568304574323E-5 26-27 2.0411420761435806E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012083561090769995 36-37 0.023758893766311278 38-39 0.010491470271378003 40-41 0.005796843496247769 42-43 0.005306969397973309 44-45 0.0015920908193919926 46-47 0.0013471537702547632 48-49 0.0029596560104081914 50-51 7.960454096959963E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.981025135031754E-4 60-61 0.001469622294823378 62-63 0.0016329136609148644 64-65 8.572796719803038E-4 66-67 0.003041301693453935 68-69 0.0026943075405095264 70-71 4.082284152287161E-5 72-73 0.0 74-75 0.0 76-77 0.09072876528458215 78-79 0.0033270615841140363 80-81 2.4493704913722963E-4 82-83 0.0 84-85 2.0411420761435806E-4 86-87 0.09279031878148718 88-89 0.43535519342066425 90-91 0.3867760120084471 92-93 0.0 94-95 0.0012859195079704558 96-97 0.0 98-99 0.0 100-101 4.6946267751302347E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2449609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.088516086624814 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92167664872579 48.21208113539087 2 12.523267149597428 15.30055613450565 3 3.797509367822591 6.959526363160162 4 1.6034974476626358 3.9182111850560295 5 0.8480891800315885 2.590425475862607 6 0.5277280783602305 1.9342875122563523 7 0.3448239128864475 1.474534680459166 8 0.24151067307830168 1.1802822909948334 9 0.18247347788912402 1.0032330590470917 >10 0.9290209995675749 10.17979859249043 >50 0.054416503099617444 2.24710489648672 >100 0.023513760291086053 2.408794159840042 >500 0.0013366491825207284 0.5116842589021091 >1k 9.35654427758622E-4 1.1687941405707425 >5k 2.0049737737684757E-4 0.9106861149773293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7746 0.3162137304361635 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7645 0.31209062344235344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6915 0.28228994913065714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.17247650543413257 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3530 0.14410463057573678 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2493 0.10177134391651892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2246852456861482E-4 2 0.0 0.0 0.0 0.0 1.2246852456861482E-4 3 0.0 0.0 0.0 0.0 1.2246852456861482E-4 4 0.0 0.0 0.0 0.0 1.2246852456861482E-4 5 0.0 0.0 0.0 0.0 1.2246852456861482E-4 6 0.0 0.0 0.0 0.0 1.2246852456861482E-4 7 0.0 0.0 0.0 4.082284152287161E-5 1.2246852456861482E-4 8 0.0 0.0 0.0 4.082284152287161E-5 2.0411420761435806E-4 9 0.0 0.0 0.0 8.164568304574323E-5 2.0411420761435806E-4 10-11 0.0 0.0 0.0 1.2246852456861482E-4 2.4493704913722963E-4 12-13 0.0 0.0 0.0 1.6329136609148645E-4 6.123426228430741E-4 14-15 0.0 0.0 0.0 1.6329136609148645E-4 7.143997266502532E-4 16-17 0.0 0.0 0.0 1.6329136609148645E-4 7.552225681731248E-4 18-19 0.0 0.0 0.0 1.6329136609148645E-4 8.572796719803038E-4 20-21 0.0 4.082284152287161E-5 0.0 1.6329136609148645E-4 9.593367757874827E-4 22-23 0.0 4.082284152287161E-5 0.0 2.6534846989866544E-4 9.797481965489185E-4 24-25 8.164568304574323E-5 4.082284152287161E-5 0.0 3.674055737058445E-4 9.797481965489185E-4 26-27 8.164568304574323E-5 4.082284152287161E-5 0.0 7.34811147411689E-4 0.0010205710380717901 28-29 8.164568304574323E-5 4.082284152287161E-5 0.0 0.0016941479231991716 0.0010613938795946617 30-31 8.164568304574323E-5 4.082284152287161E-5 0.0 0.005551906447110539 0.0010613938795946617 32-33 1.0205710380717901E-4 4.082284152287161E-5 0.0 0.013267423494933272 0.0010613938795946617 34-35 1.2246852456861482E-4 4.082284152287161E-5 0.0 0.024697819121337325 0.0010613938795946617 36-37 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.04421113736926995 0.0010613938795946617 38-39 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.07170532113492398 0.0010818053003560976 40-41 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.10336343473591092 0.0011022167211175334 42-43 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.13796079292654462 0.0011226281418789692 44-45 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.18057983947642256 0.001143039562640405 46-47 1.6329136609148645E-4 4.082284152287161E-5 0.0 0.23473133875651175 0.001143039562640405 48-49 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.2904953402767544 0.001245096666447584 50-51 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.3527501735991336 0.0013063309287318916 52-53 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.4171277946807021 0.0013471537702547632 54-55 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.49044561805577946 0.0013471537702547632 56-57 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.5817663145424433 0.0013879766117776348 58-59 2.0411420761435806E-4 4.082284152287161E-5 0.0 0.6857216804804358 0.0013879766117776348 60-61 2.2452562837579383E-4 4.082284152287161E-5 0.0 0.7950044272371632 0.0013879766117776348 62-63 2.4493704913722963E-4 4.082284152287161E-5 0.0 0.9103901888015598 0.001469622294823378 64-65 2.4493704913722963E-4 6.123426228430742E-5 0.0 1.0335935245175862 0.001469622294823378 66-67 2.4493704913722963E-4 8.164568304574323E-5 0.0 1.178963663180532 0.001469622294823378 68-69 2.4493704913722963E-4 8.164568304574323E-5 0.0 1.3517259285053247 0.0014900337155848138 70-71 2.6534846989866544E-4 8.164568304574323E-5 0.0 1.5405315705486058 0.0015104451363462496 72-73 2.8575989066010124E-4 8.164568304574323E-5 0.0 1.733684845214073 0.0016125022401534286 74-75 2.8575989066010124E-4 8.164568304574323E-5 0.0 1.9392278522817317 0.0017349707647220435 76-77 2.8575989066010124E-4 8.164568304574323E-5 0.0 2.167345890711538 0.0017553821854834793 78-79 2.8575989066010124E-4 8.164568304574323E-5 0.0 2.436613353396399 0.001775793606244915 80-81 2.8575989066010124E-4 8.164568304574323E-5 0.0 2.737232758370826 0.0018166164477677867 82-83 2.8575989066010124E-4 8.164568304574323E-5 0.0 3.041403750557742 0.0018370278685292225 84-85 2.8575989066010124E-4 8.164568304574323E-5 0.0 3.3498815525253214 0.001877850710052094 86-87 2.8575989066010124E-4 8.164568304574323E-5 0.0 3.680403688915251 0.0019390849723364013 88-89 2.8575989066010124E-4 8.164568304574323E-5 0.0 4.04601305759409 0.0020003192346207087 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8540 0.0 21.81379 1 TACATGG 8395 0.0 21.19997 2 GTAAGCG 365 0.0 20.154516 94-95 GAGTACT 7740 0.0 20.051609 12-13 TTTGCGT 450 0.0 20.038527 3 ACATGGG 8450 0.0 19.77018 3 GTACTTT 8065 0.0 19.302425 14-15 AGAGTAC 12530 0.0 18.63616 10-11 GTGTAGC 1465 0.0 18.537426 1 GTATCAA 18785 0.0 18.36283 1 AGTACTT 8190 0.0 18.341389 12-13 CATGGGG 4930 0.0 17.616875 4 CCGTGCG 190 1.878636E-4 17.485497 9 GGTATCA 15340 0.0 17.455133 1 CTTTGCG 530 0.0 16.118378 2 GGACCGT 265 8.008286E-6 16.118378 6 ACTTTTT 9630 0.0 16.115751 16-17 ATCAACG 21105 0.0 16.011032 3 TCAACGC 21165 0.0 15.988067 4 TATCAAC 21225 0.0 15.98759 2 >>END_MODULE