FastQCFastQC Report
Thu 26 May 2016
SRR938892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453403
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77490.31584700923574316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69160.2818941690378629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64340.2622479877949118No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38090.1552537434738606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.1545200686556591No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27450.11188540977572782No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25020.10198079973000766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80500.021.970361
GAGTACT73800.021.22940312-13
TACATGG79250.021.1429542
ACATGGG80100.020.2094883
GTACTTT78000.019.75292414-15
GGTATCA137350.019.7120441
GTATCAA171900.019.3084051
AGTACTT76350.019.21846812-13
AGAGTAC118900.019.06883610-11
CATGGGG47850.017.904344
ACTTTTT92400.016.95733316-17
TATCAAC195050.016.8170172
TCAACGC196500.016.6447454
ATCAACG196950.016.6307493
CAACGCA197550.016.5083585
GTGTAGC15050.016.3829121
AACGCAG200400.016.248646
GAGTACA70550.016.1301521
TACTTTT91450.015.99372914-15
AGTACAT62400.015.7775822
Produced by FastQC (version 0.11.5)