##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938892_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2453403 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.596434014305842 33.0 31.0 34.0 30.0 34.0 2 31.67443872857415 33.0 31.0 34.0 30.0 34.0 3 31.715691225616013 33.0 31.0 34.0 28.0 34.0 4 35.249906354561396 37.0 35.0 37.0 33.0 37.0 5 35.27470497101373 37.0 35.0 37.0 33.0 37.0 6 35.34953531890195 37.0 35.0 37.0 33.0 37.0 7 35.25563431690595 37.0 35.0 37.0 32.0 37.0 8 35.23423791362446 37.0 35.0 37.0 32.0 37.0 9 36.810345874689155 39.0 37.0 39.0 33.0 39.0 10-11 36.68602691852908 39.0 37.0 39.0 32.0 39.0 12-13 36.54811256039061 39.0 37.0 39.0 32.0 39.0 14-15 37.71482813871182 40.0 38.0 41.0 32.0 41.0 16-17 37.62852474705542 40.0 37.0 41.0 32.0 41.0 18-19 37.5494847360992 40.0 37.0 41.0 32.0 41.0 20-21 37.41115075672444 40.0 37.0 41.0 32.0 41.0 22-23 37.50754319612392 40.0 37.0 41.0 32.0 41.0 24-25 37.43608530681669 40.0 37.0 41.0 31.5 41.0 26-27 37.343876444269455 40.0 37.0 41.0 31.0 41.0 28-29 37.20722196883268 40.0 37.0 41.0 31.0 41.0 30-31 37.01783828421176 40.0 37.0 41.0 30.0 41.0 32-33 36.81851473239415 40.0 36.0 41.0 30.0 41.0 34-35 36.49245150511351 39.5 36.0 41.0 30.0 41.0 36-37 36.22364324165251 39.0 35.0 41.0 29.5 41.0 38-39 36.010599970734525 39.0 35.0 41.0 28.5 41.0 40-41 35.85882119651765 39.0 35.0 41.0 27.5 41.0 42-43 35.64773642976714 39.0 35.0 40.5 27.0 41.0 44-45 35.57338154392083 39.0 35.0 41.0 26.5 41.0 46-47 35.348637586242454 39.0 35.0 41.0 25.0 41.0 48-49 35.162529759684816 39.0 35.0 41.0 24.5 41.0 50-51 34.015798260620045 38.0 33.5 40.0 23.5 40.5 52-53 34.17434029386938 38.0 33.5 39.5 23.5 40.5 54-55 34.65908454501768 38.5 34.0 40.0 23.5 41.0 56-57 34.53110292112629 38.0 34.0 40.0 23.0 41.0 58-59 34.30908273121048 38.0 34.0 40.0 23.0 41.0 60-61 34.02443850439573 38.0 34.0 40.0 22.0 41.0 62-63 33.660029558943236 37.5 33.0 40.0 21.0 41.0 64-65 33.23859614584314 37.0 33.0 40.0 19.5 41.0 66-67 32.84104384807551 36.0 32.0 39.0 18.0 41.0 68-69 32.43786935941629 36.0 32.0 39.0 17.0 41.0 70-71 32.00742641954868 35.0 31.5 38.5 13.0 40.0 72-73 31.56084385647201 35.0 31.0 37.5 9.5 40.0 74-75 31.079237695560003 35.0 31.0 37.0 7.5 39.0 76-77 30.65650608562882 35.0 30.5 36.5 6.5 39.0 78-79 30.215104897157133 34.5 30.0 36.0 2.0 38.5 80-81 29.760179636203265 34.0 29.5 35.5 2.0 37.5 82-83 29.35348065523683 34.0 29.0 35.0 2.0 37.0 84-85 28.98014064546265 34.0 29.0 35.0 2.0 36.5 86-87 28.578737573892262 34.0 29.0 35.0 2.0 36.0 88-89 28.13826957902962 34.0 28.0 35.0 2.0 36.0 90-91 27.687964635243375 34.0 27.0 35.0 2.0 35.0 92-93 27.245819989622575 33.5 26.0 35.0 2.0 35.0 94-95 26.735290533190025 33.0 25.0 35.0 2.0 35.0 96-97 26.096256913356672 33.0 24.5 35.0 2.0 35.0 98-99 25.326645072171182 32.5 20.5 35.0 2.0 35.0 100-101 22.6085767401442 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32022394712298663 1101 2 0.3599388872041125 1101 3 0.3390277754288995 1101 4 0.28445669890755454 1101 5 0.21678614808900676 1101 6 0.27715535148716697 1101 7 0.3237075916131076 1101 8 0.3630902668025229 1101 9 0.33112086375228245 1101 10-11 0.5082043958978062 1101 12-13 0.6050642796214447 1101 14-15 0.29600450173797554 1101 16-17 0.5941665742698063 1101 18-19 0.4902170871367133 1101 20-21 0.704435099582561 1101 22-23 0.5987107176791397 1101 24-25 0.5862019563214105 1101 26-27 0.6111119328837447 1101 28-29 0.5875348646841729 1101 30-31 0.6620792355588492 1101 32-33 0.7447692248068307 1101 34-35 0.5628853492804708 1101 36-37 0.4322386562739027 1101 38-39 0.5133079199318189 1101 40-41 0.43208094835787847 1101 42-43 0.45292102002100876 1101 44-45 0.580710268212016 1101 46-47 0.6836275993286804 1101 48-49 0.876522984714363 1101 50-51 0.7754655428128316 1101 52-53 0.6474543369220811 1101 54-55 0.5439176129710148 1101 56-57 0.5973098128457508 1101 58-59 0.6411437857697422 1101 60-61 0.6373609031235432 1101 62-63 0.6867004768059317 1101 64-65 0.6906738790109799 1101 66-67 0.5700887405856321 1101 68-69 0.6232009923804966 1101 70-71 0.602903077795311 1101 72-73 0.42215142719260257 1101 74-75 0.31082681038707705 1101 76-77 0.2958332430032762 1101 78-79 0.28293754678143657 1101 80-81 0.2708881327159496 1101 82-83 0.28029144917054793 1101 84-85 -0.008156459193681798 1101 86-87 0.0036558151932304384 1101 88-89 0.02029755091282226 1101 90-91 0.0533307023824392 1101 92-93 0.24905030317637866 1101 94-95 0.35179263037712616 1101 96-97 0.3200572980296066 1101 98-99 0.17599699462254748 1101 100-101 0.19767695044037836 1102 1 0.21112952755813907 1102 2 0.1093485199999158 1102 3 0.0917302588094131 1102 4 0.15968732996925183 1102 5 0.20616606971694296 1102 6 0.13043740305368345 1102 7 0.2265636679889127 1102 8 0.11899165736176798 1102 9 0.312214619092515 1102 10-11 0.17860413493197314 1102 12-13 0.24859244680706638 1102 14-15 0.43358211464855145 1102 16-17 0.3188425187275996 1102 18-19 0.3467322233877539 1102 20-21 0.3280629219332454 1102 22-23 0.3952365473738766 1102 24-25 0.3123959367500433 1102 26-27 0.3157985099457079 1102 28-29 0.3456475040805316 1102 30-31 0.42282855321658275 1102 32-33 0.4508802523132829 1102 34-35 0.3377999905123872 1102 36-37 0.28847366223528326 1102 38-39 0.3548843547026195 1102 40-41 0.21789517196214803 1102 42-43 0.24012981111968656 1102 44-45 0.41578295625137685 1102 46-47 0.4242343771038577 1102 48-49 0.40900718298613015 1102 50-51 0.20724258880626678 1102 52-53 0.2428534183524107 1102 54-55 0.33037315689348645 1102 56-57 0.48434877216038785 1102 58-59 0.31853658247568006 1102 60-61 0.27417354829965035 1102 62-63 0.23834492481326208 1102 64-65 0.2687435334835513 1102 66-67 0.3272325997914862 1102 68-69 0.16253921208316768 1102 70-71 0.2675989310975808 1102 72-73 0.22251497922677999 1102 74-75 0.21267706602945324 1102 76-77 0.26073562864686295 1102 78-79 0.06912253369467436 1102 80-81 -0.11274667544535433 1102 82-83 0.06253134985178832 1102 84-85 0.05362224557189421 1102 86-87 0.153118789949648 1102 88-89 0.0284390443553022 1102 90-91 0.03473545513144671 1102 92-93 -0.15815338455860228 1102 94-95 -0.13196410737815611 1102 96-97 -0.1091268204886795 1102 98-99 -0.06594261640880461 1102 100-101 0.2493170144221004 1103 1 0.1033758079587237 1103 2 0.23525908914783145 1103 3 0.25467035641078795 1103 4 0.3307510251710113 1103 5 0.23971011632518469 1103 6 0.21873997769326792 1103 7 0.27204309904337265 1103 8 0.20324890963367892 1103 9 0.14120053227998142 1103 10-11 0.3145109736006475 1103 12-13 0.36361173587408047 1103 14-15 0.3932110044969761 1103 16-17 0.37399200384803777 1103 18-19 0.4442616897859537 1103 20-21 0.45892179358774854 1103 22-23 0.6315092165720699 1103 24-25 0.5406479860511197 1103 26-27 0.39474345591644067 1103 28-29 0.37294268456283675 1103 30-31 0.3820440099728373 1103 32-33 0.5357088069737586 1103 34-35 0.5023191392610826 1103 36-37 0.5512101684400079 1103 38-39 0.5965328181947029 1103 40-41 0.5689843089248257 1103 42-43 0.3548389134352803 1103 44-45 0.4871347741122918 1103 46-47 0.3349642597826943 1103 48-49 0.1339302765509558 1103 50-51 0.17755465422916217 1103 52-53 0.2383749628102123 1103 54-55 0.256933040756131 1103 56-57 0.3641409291277782 1103 58-59 0.35669376155282606 1103 60-61 0.38024514950264887 1103 62-63 0.2863317286235443 1103 64-65 -0.020607157174474366 1103 66-67 0.04737564746227463 1103 68-69 0.0872491686659771 1103 70-71 0.06848097542169285 1103 72-73 0.10271474880745046 1103 74-75 0.22211376366524505 1103 76-77 0.11107687884632611 1103 78-79 0.0943951425389713 1103 80-81 0.024246712311544627 1103 82-83 0.1646252357879554 1103 84-85 0.089013353993451 1103 86-87 0.15099753880816635 1103 88-89 0.15752392359290468 1103 90-91 0.0798943121183413 1103 92-93 0.09615811278555952 1103 94-95 0.04072090460218192 1103 96-97 -0.07559504165899611 1103 98-99 0.02501311159609898 1103 100-101 0.2527185022797731 1104 1 0.17584030480373158 1104 2 0.2756797999463778 1104 3 0.22345739152089905 1104 4 0.15974376680388502 1104 5 0.267739012367322 1104 6 0.3202216594556617 1104 7 0.24299871515640348 1104 8 0.2831562613541081 1104 9 0.364378277367301 1104 10-11 0.2560797135385968 1104 12-13 0.3341329737063461 1104 14-15 0.4667365295668233 1104 16-17 0.20749798502671268 1104 18-19 0.3862108574169625 1104 20-21 0.31833184077974863 1104 22-23 0.3672318600900226 1104 24-25 0.4755837562212548 1104 26-27 0.426330406638165 1104 28-29 0.3000032793935645 1104 30-31 0.2743770677973103 1104 32-33 0.47865046858386506 1104 34-35 0.421606286477477 1104 36-37 0.34282696287323944 1104 38-39 0.292318248386664 1104 40-41 0.36730350207454876 1104 42-43 0.3150370686651698 1104 44-45 0.5791685716950568 1104 46-47 0.5972914322511897 1104 48-49 0.5430122630133809 1104 50-51 0.48746713975510403 1104 52-53 0.5224861836914343 1104 54-55 0.545408891023655 1104 56-57 0.5211707801758436 1104 58-59 0.48770314573415163 1104 60-61 0.4381331459352893 1104 62-63 0.48257345731847323 1104 64-65 0.37643364861647655 1104 66-67 0.5632176157228699 1104 68-69 0.622328234460106 1104 70-71 0.48873073353875185 1104 72-73 0.5682811217192807 1104 74-75 0.4572122624073849 1104 76-77 0.22258334924856626 1104 78-79 0.3246358516304255 1104 80-81 0.24537272460082704 1104 82-83 0.205956396305357 1104 84-85 0.2827859709655698 1104 86-87 0.40951551347983894 1104 88-89 0.24039229471640056 1104 90-91 0.3288842372263332 1104 92-93 0.36258946661248004 1104 94-95 0.39488682941098574 1104 96-97 0.39420971805331817 1104 98-99 0.5073820953227042 1104 100-101 0.9790360284189674 1105 1 0.213052572985859 1105 2 0.11861030829480512 1105 3 0.20793820341725322 1105 4 0.21220020618482494 1105 5 0.22195758341608496 1105 6 0.2508790413545512 1105 7 0.22845976964828196 1105 8 0.29015555595246667 1105 9 0.25110509584400376 1105 10-11 0.15366644459757595 1105 12-13 0.39812613470630254 1105 14-15 0.40439047876910905 1105 16-17 0.43066127063941906 1105 18-19 0.37570751665658264 1105 20-21 0.4279738490049212 1105 22-23 0.346806180373342 1105 24-25 0.3498829953763405 1105 26-27 0.35084055367103417 1105 28-29 0.298457518991178 1105 30-31 0.414610527765106 1105 32-33 0.31270943833147413 1105 34-35 0.3354496310068029 1105 36-37 0.2825093157088432 1105 38-39 0.19976573432218458 1105 40-41 0.0459970116579953 1105 42-43 0.03194103638666945 1105 44-45 -0.08737844590232413 1105 46-47 0.021838001868381696 1105 48-49 0.07428237131514948 1105 50-51 0.0471565413285262 1105 52-53 0.06038784165802724 1105 54-55 0.07939116219876752 1105 56-57 0.0015660162425774615 1105 58-59 0.0045252770113535234 1105 60-61 0.11104250279162642 1105 62-63 0.049951895183745876 1105 64-65 0.01370209907481268 1105 66-67 -0.15408295347238266 1105 68-69 -0.2925142677749548 1105 70-71 -0.20100805670710642 1105 72-73 -0.14070515681988383 1105 74-75 -0.03988809518497405 1105 76-77 -0.030639741543787125 1105 78-79 -0.07104889911329337 1105 80-81 -0.3071916628742386 1105 82-83 -0.333859558792458 1105 84-85 -0.18485889722426307 1105 86-87 -0.067017696966861 1105 88-89 -0.011370637544452222 1105 90-91 -0.045375236208670344 1105 92-93 0.10051920691945426 1105 94-95 0.15455654167433153 1105 96-97 0.10061579960436617 1105 98-99 0.06002337492023102 1105 100-101 0.3697980117571298 1106 1 0.16683770282921273 1106 2 0.18690583459781251 1106 3 0.22823452757968 1106 4 0.08002011732478564 1106 5 0.11088132410901608 1106 6 0.15394256660476913 1106 7 0.14714115192939659 1106 8 0.16057229970076037 1106 9 0.1381935187177703 1106 10-11 0.19002313461093223 1106 12-13 0.28653200744773244 1106 14-15 0.21770334542357972 1106 16-17 0.25548823746107274 1106 18-19 0.21058515237450592 1106 20-21 0.3366639023230178 1106 22-23 0.2846558252122122 1106 24-25 0.16358729889088153 1106 26-27 0.24999952853880814 1106 28-29 0.3021317718678276 1106 30-31 0.1484976258339472 1106 32-33 0.1699530613987008 1106 34-35 0.1461919330930499 1106 36-37 0.17290777001123558 1106 38-39 0.2653417002884453 1106 40-41 0.13812617669260163 1106 42-43 0.27658123658186184 1106 44-45 0.4583152835633868 1106 46-47 0.2712345259435125 1106 48-49 0.4658776147501129 1106 50-51 0.32364592197809827 1106 52-53 0.2545157203337851 1106 54-55 0.15717651321040904 1106 56-57 0.2574007938874914 1106 58-59 0.41772688775035505 1106 60-61 0.3726778665669528 1106 62-63 0.2807664938063752 1106 64-65 0.14264174528134532 1106 66-67 0.3318950805895966 1106 68-69 0.3726894843691184 1106 70-71 0.33780654673850563 1106 72-73 0.2073437909599427 1106 74-75 0.19358672384114683 1106 76-77 0.1649821426790936 1106 78-79 0.3453550010275066 1106 80-81 0.33231074982371567 1106 82-83 0.3854507578812516 1106 84-85 0.3603731176084821 1106 86-87 0.4791058809847968 1106 88-89 0.5777126405829343 1106 90-91 0.646754118300322 1106 92-93 0.6005396184784964 1106 94-95 0.6519271539211964 1106 96-97 0.8824667886778919 1106 98-99 0.6443594583881769 1106 100-101 0.803545019353674 1107 1 0.01317798963192729 1107 2 -0.03548043403123202 1107 3 0.05175128577852206 1107 4 0.07461814544882373 1107 5 -0.07333079247304397 1107 6 -0.015253867331544768 1107 7 -0.08386670913626659 1107 8 0.0666910208483813 1107 9 -0.08435893273642137 1107 10-11 -0.006425909236952521 1107 12-13 0.04929872830693682 1107 14-15 -9.44238830946631E-4 1107 16-17 0.04339053981424712 1107 18-19 0.041688208044035946 1107 20-21 0.015047087642614088 1107 22-23 -0.03706451038095082 1107 24-25 0.05128564959500892 1107 26-27 0.21258865008746852 1107 28-29 0.3674752707373372 1107 30-31 0.29949695457894876 1107 32-33 0.24430116291443937 1107 34-35 0.11064202760094588 1107 36-37 0.11162965584294682 1107 38-39 0.18661147098018205 1107 40-41 0.12910045784029478 1107 42-43 0.235926874540219 1107 44-45 0.4195132248191271 1107 46-47 0.42225059314455393 1107 48-49 0.38367196693752703 1107 50-51 0.2519092771200704 1107 52-53 0.29281897308791116 1107 54-55 0.2951528081231203 1107 56-57 0.5016001892429571 1107 58-59 0.2865991965070478 1107 60-61 0.23777758484586542 1107 62-63 0.18228892076232484 1107 64-65 0.25710059528933016 1107 66-67 0.23466233364586486 1107 68-69 0.22298657050435367 1107 70-71 0.3033424090951726 1107 72-73 0.20260122841391137 1107 74-75 0.20343511634849776 1107 76-77 0.3011668219273602 1107 78-79 0.44991146627761225 1107 80-81 0.4493405408419484 1107 82-83 0.5205746482074574 1107 84-85 0.5797135022667845 1107 86-87 0.5822245557797707 1107 88-89 0.410867659287085 1107 90-91 0.6136051941109351 1107 92-93 0.5721016683642439 1107 94-95 0.6139586702879711 1107 96-97 0.6141150888233042 1107 98-99 0.8325405633322909 1107 100-101 0.9364056129079117 1108 1 0.2608767661140874 1108 2 0.2327663564707514 1108 3 0.16310577458108 1108 4 0.34210824617334623 1108 5 0.26723125194656916 1108 6 0.25850338031293774 1108 7 0.2117957645677464 1108 8 0.24863498914249504 1108 9 0.4430326907976365 1108 10-11 0.36428748195121585 1108 12-13 0.35156703502627806 1108 14-15 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-1.8803097332414538 2316 62-63 -2.28018509171169 2316 64-65 -2.5098779216891884 2316 66-67 -2.5752129262293906 2316 68-69 -2.767103519585735 2316 70-71 -2.5366437308909653 2316 72-73 -2.4268166510039677 2316 74-75 -2.610528847615466 2316 76-77 -2.6009814802209448 2316 78-79 -2.229818263452117 2316 80-81 -2.427582536081129 2316 82-83 -2.6650455874127807 2316 84-85 -2.9047979322446515 2316 86-87 -2.873492699937483 2316 88-89 -3.099111550692122 2316 90-91 -3.411100280594539 2316 92-93 -3.6510099047473297 2316 94-95 -3.8336158272865255 2316 96-97 -4.119876595168382 2316 98-99 -4.660286879495153 2316 100-101 -4.8662970897947915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10229.0 3 3242.0 4 3264.0 5 3436.0 6 4053.0 7 4818.0 8 5788.0 9 7043.0 10 8351.0 11 10125.0 12 12217.0 13 16315.0 14 15947.0 15 14693.0 16 17599.0 17 21856.0 18 19865.0 19 15908.0 20 16378.0 21 17627.0 22 19486.0 23 21456.0 24 23402.0 25 27511.0 26 31233.0 27 36185.0 28 42443.0 29 49868.0 30 59219.0 31 71668.0 32 87646.0 33 111978.0 34 148697.0 35 211304.0 36 319567.0 37 477462.0 38 420327.0 39 65181.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31042438309139 18.931543347622643 14.848196354488788 22.90983591479718 2 15.152658676087826 20.1331173864443 40.852106298088295 23.862117639379573 3 19.81618184028676 24.048169039597443 29.545170815929712 26.59047830418609 4 12.972798070166991 16.757712436275444 37.20167685253115 33.067812641026414 5 14.077351393743436 36.83714864665256 34.175460616353114 14.910039343250888 6 31.82119501122039 35.71971422988479 17.64495433315887 14.814136425735954 7 28.304739555369878 32.438611636495374 20.853358225396104 18.403290582738638 8 25.61367951507793 35.128700333469595 19.704936116951792 19.552684034500682 9 25.672990697139298 17.767476624532755 20.778687220377677 35.78084545795027 10-11 25.015380008599752 26.42919851797095 27.100044304215753 21.455377169213545 12-13 26.110437382132844 23.78814969090629 27.599900861545805 22.50151206541506 14-15 22.896334379141983 25.307678482284736 26.235033780523803 25.56095335804948 16-17 22.236328766430304 28.3974662071886 26.809439671473456 22.556765354907643 18-19 22.08023583173528 27.349932677791834 28.72748786160186 21.842343628871028 20-21 23.071356715847593 26.300095660747697 28.621842598593915 22.006705024810795 22-23 22.563133435950842 26.195764660945986 28.75044276244591 22.49065914065726 24-25 22.70274892035051 26.339162274027622 28.673098887384036 22.284989918237837 26-27 22.41900775766456 26.389487206434687 28.93913962673828 22.252365409162472 28-29 22.11577408372129 26.910449908328765 28.771789134739876 22.201986873210064 30-31 22.468772514303563 26.473893048654258 28.525432606189977 22.5319018308522 32-33 22.371316088426934 26.455100185419738 28.69554111225729 22.478042613896037 34-35 22.810505213182683 26.53294453626989 28.47322682945242 22.183323421095004 36-37 22.674607045353763 26.291997729328862 28.69522718294299 22.33816804237438 38-39 22.373366626826254 26.690397367622186 28.656071754555732 22.280164250995824 40-41 22.650012396448673 26.584501775458307 28.506402099814977 22.259083728278043 42-43 22.29324283721561 26.673816778154023 28.19263818618628 22.840302198444082 44-45 22.59365978876349 26.588117505587178 28.1852241041349 22.63299860151443 46-47 22.92447487343403 26.82770199568285 27.87072981406245 22.377093316820673 48-49 22.777584149646753 26.81637765959628 27.871256883480054 22.534781307276912 50-51 22.390871301413966 27.040276311152635 28.077324646129064 22.49152774130434 52-53 22.701764096886947 27.192185081542075 27.539223223346355 22.566827598224627 54-55 22.591638485898958 26.85812164703186 27.70749070326774 22.842749163801443 56-57 22.690376520162577 27.065169922734356 27.600637667509144 22.643815889593924 58-59 22.972476455097418 26.891404816994342 27.60985759839637 22.52626112951187 60-61 22.68767427313739 26.886184766494992 28.122988959168215 22.303152001199404 62-63 22.40274640348265 26.881332093415267 28.11683652000065 22.599084983101434 64-65 22.350197769310622 27.170413358465513 27.87521065081696 22.6041782214069 66-67 22.5157781347258 27.045456735910616 27.46696573196457 22.97179939739901 68-69 22.32459449322445 26.95999470104045 27.889773438719196 22.825637367015904 70-71 22.641876217405223 26.814504098396792 27.68278226319924 22.860837420998745 72-73 22.579286585279583 26.631902289572963 27.925150240218265 22.86366088492919 74-75 22.338637567295645 27.337086463818284 27.425826395741957 22.898449573144113 76-77 22.50609935975429 26.914038644346185 27.455595779474706 23.12426621642482 78-79 22.57086219271598 26.960528521753318 27.57287280201053 22.895736483520178 80-81 22.661762502738558 26.94332141039732 27.505727661505297 22.88918842535882 82-83 22.575485148936973 26.891730351560287 27.726910485317013 22.805874014185722 84-85 22.557146986292103 26.962738381496493 27.501009591632997 22.97910504057841 86-87 22.45374465141305 27.004801557815984 27.655804658074768 22.8856491326962 88-89 22.540158728401575 26.925197897998736 27.358796134003605 23.17584723959608 90-91 22.541040157609952 26.765828295939787 27.491713237312748 23.201418309137512 92-93 22.71595414991948 26.901510743011293 27.485422205490412 22.89711290157882 94-95 22.6254015264258 26.7179671823784 27.572198890550652 23.08443240064515 96-97 22.36592849724891 26.681743762500886 27.802274071666783 23.150053668583425 98-99 22.4113770966972 26.829399322036124 27.38068582123767 23.378537760029 100-101 22.698709752674066 26.149981437281276 27.815173131433273 23.33613567861138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2597.0 1 1484.0 2 430.5 3 479.5 4 416.0 5 422.5 6 723.0 7 1466.5 8 2383.5 9 2885.5 10 2712.0 11 2485.0 12 2656.5 13 2905.5 14 3110.5 15 3376.5 16 3715.0 17 3920.0 18 4040.0 19 4276.5 20 4647.5 21 5120.0 22 5946.5 23 7223.0 24 8560.5 25 10357.0 26 13360.5 27 17079.0 28 20274.5 29 23416.0 30 26813.5 31 31047.0 32 35452.0 33 39654.5 34 44595.5 35 48936.0 36 54012.5 37 58925.5 38 62731.0 39 65786.5 40 68144.5 41 71715.0 42 74865.0 43 77510.0 44 81488.0 45 84802.0 46 87271.5 47 94477.5 48 107320.5 49 107274.0 50 96192.5 51 91335.5 52 89197.5 53 87257.0 54 84820.5 55 79131.0 56 72064.5 57 65046.5 58 58742.5 59 52490.0 60 44840.0 61 36792.0 62 28853.0 63 22237.5 64 16883.5 65 12532.5 66 9080.5 67 6849.0 68 4962.0 69 3363.0 70 2370.0 71 1479.5 72 944.0 73 644.5 74 425.5 75 279.5 76 179.5 77 103.5 78 74.0 79 51.0 80 39.0 81 32.0 82 21.5 83 19.5 84 15.0 85 9.5 86 7.5 87 8.5 88 7.5 89 6.5 90 4.5 91 3.5 92 4.0 93 1.5 94 1.5 95 3.0 96 1.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2874374898864964 2 0.0889784515629923 3 0.09313594219946743 4 0.106831205472562 5 0.10862463280594341 6 0.08404652639619337 7 0.09321746162371206 8 0.08987516522968302 9 0.09492936953284886 10-11 0.08840781559328002 12-13 0.0931563220555286 14-15 0.10224573785880264 16-17 0.09032352206302838 18-19 0.10202155944212998 20-21 0.10412068461642869 22-23 0.11734721120011674 24-25 0.12988082267772558 26-27 0.1322856456929416 28-29 0.12515269607153817 30-31 0.11728607163193328 32-33 0.11196692919997245 34-35 0.38536269826033476 36-37 0.5900579725385516 38-39 0.5742024445229749 40-41 0.5375390834689613 42-43 0.5630546632575243 44-45 0.5843312329853676 46-47 0.5731834517199172 48-49 0.5948064790008001 50-51 0.5874901106748464 52-53 0.590119112106735 54-55 0.5803979207655652 56-57 0.5532519524921099 58-59 0.5477086316434765 60-61 0.49694241019514523 62-63 0.5704117912956004 64-65 0.6044461509177254 66-67 0.6170816616756399 68-69 0.6190788875696329 70-71 0.6143100012513231 72-73 0.6069732530693082 74-75 0.6039162746601353 76-77 0.5876531495233355 78-79 0.5642774546211935 80-81 0.5587137539164988 82-83 0.572633195606266 84-85 0.58278236392472 86-87 0.5743247236593417 88-89 0.5866952962884614 90-91 0.6001663811448833 92-93 0.6087055408345062 94-95 0.6071566717738586 96-97 0.6042831120692361 98-99 0.6083387034254054 100-101 0.5859004819020764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2453403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.905210148745795 #Duplication Level Percentage of deduplicated Percentage of total 1 80.33088392447937 50.53231134703881 2 11.689699643154013 14.70686025256644 3 3.574895187592416 6.74638599105723 4 1.5000427039316806 3.774420060916609 5 0.8037024974043565 2.527853724814643 6 0.49022491740412166 1.8502620869674695 7 0.32057779674211195 1.4116209571159126 8 0.20472713569153114 1.0302722795061248 9 0.1545762213559732 0.8751284719556888 >10 0.8571013726379364 9.73019086208646 >50 0.05091089243076586 2.191311848005101 >100 0.020383639116320358 2.1701438561133055 >500 0.0011695207162111445 0.45694490058242343 >1k 9.096272237146369E-4 1.133942694248513 >5k 1.9492011936742215E-4 0.8623506670252803 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7749 0.31584700923574316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6916 0.2818941690378629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6434 0.2622479877949118 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3809 0.1552537434738606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3791 0.1545200686556591 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2745 0.11188540977572782 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2502 0.10198079973000766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.151942424461045E-5 2 0.0 0.0 0.0 0.0 8.151942424461045E-5 3 0.0 0.0 0.0 0.0 8.151942424461045E-5 4 0.0 0.0 0.0 0.0 8.151942424461045E-5 5 4.075971212230522E-5 0.0 0.0 0.0 8.151942424461045E-5 6 4.075971212230522E-5 0.0 0.0 0.0 1.2227913636691566E-4 7 4.075971212230522E-5 0.0 0.0 0.0 1.2227913636691566E-4 8 4.075971212230522E-5 0.0 0.0 0.0 1.2227913636691566E-4 9 4.075971212230522E-5 0.0 0.0 4.075971212230522E-5 1.2227913636691566E-4 10-11 4.075971212230522E-5 0.0 0.0 1.0189928030576305E-4 1.2227913636691566E-4 12-13 6.113956818345784E-5 0.0 0.0 1.4265899242806826E-4 4.075971212230522E-4 14-15 1.0189928030576305E-4 0.0 0.0 2.037985606115261E-4 4.891165454676627E-4 16-17 1.4265899242806826E-4 0.0 0.0 3.0569784091728915E-4 5.706359697122732E-4 18-19 1.630388484892209E-4 0.0 0.0 4.075971212230522E-4 5.706359697122732E-4 20-21 1.630388484892209E-4 0.0 0.0 4.4835683334535747E-4 6.113956818345783E-4 22-23 1.630388484892209E-4 0.0 0.0 6.31775537895731E-4 6.521553939568836E-4 24-25 2.037985606115261E-4 0.0 0.0 6.521553939568836E-4 6.521553939568836E-4 26-27 2.037985606115261E-4 0.0 0.0 0.0010597525151799358 7.540546742626467E-4 28-29 2.445582727338313E-4 0.0 0.0 0.0023233035909713978 8.763338106295624E-4 30-31 2.6493812879498395E-4 0.0 0.0 0.0061343366744069355 9.170935227518675E-4 32-33 2.853179848561366E-4 0.0 0.0 0.013939821545828388 9.374733788130201E-4 34-35 2.853179848561366E-4 0.0 0.0 0.025862037341602664 0.001100512227302241 36-37 2.853179848561366E-4 0.0 0.0 0.04516176103151419 0.0011208920833633935 38-39 3.260776969784418E-4 0.0 0.0 0.07316368325953787 0.0011820316515468515 40-41 3.260776969784418E-4 0.0 0.0 0.10465056087401867 0.0011820316515468515 42-43 3.260776969784418E-4 0.0 0.0 0.13799200539006434 0.0011820316515468515 44-45 3.260776969784418E-4 0.0 0.0 0.17944463261844873 0.0012227913636691568 46-47 3.260776969784418E-4 0.0 0.0 0.2324526382335067 0.0012839309318526146 48-49 3.260776969784418E-4 0.0 0.0 0.28802850571226984 0.0013450705000360723 50-51 3.260776969784418E-4 0.0 0.0 0.3521639127367171 0.0014265899242806828 52-53 3.8721726516189963E-4 0.0 0.0 0.4190302204733588 0.001467349636402988 54-55 4.075971212230522E-4 0.0 0.0 0.4939261914980947 0.001467349636402988 56-57 4.075971212230522E-4 0.0 0.0 0.5871844128339291 0.0014877294924641406 58-59 4.075971212230522E-4 0.0 0.0 0.6908363607609511 0.0015081093485252931 60-61 4.075971212230522E-4 8.151942424461045E-5 0.0 0.8032720266503302 0.0015081093485252931 62-63 4.075971212230522E-4 8.151942424461045E-5 0.0 0.9237577356838644 0.0015488690606475985 64-65 4.075971212230522E-4 8.151942424461045E-5 0.0 1.0476468806796113 0.0016100086288310562 66-67 4.075971212230522E-4 8.151942424461045E-5 0.0 1.1915898040395319 0.0016507683409533614 68-69 4.075971212230522E-4 8.151942424461045E-5 0.0 1.361435524453178 0.0016711481970145142 70-71 4.075971212230522E-4 8.151942424461045E-5 0.0 1.5533730088371132 0.0017526676212591247 72-73 4.075971212230522E-4 8.151942424461045E-5 0.0 1.7513225507590886 0.001834187045503735 74-75 4.075971212230522E-4 8.151942424461045E-5 0.0 1.9601956955298416 0.0019360863258094981 76-77 4.075971212230522E-4 8.151942424461045E-5 0.0 2.1860248805434734 0.0020379856061152613 78-79 4.075971212230522E-4 8.151942424461045E-5 0.0 2.4534493517779183 0.0021602647424821767 80-81 4.4835683334535747E-4 8.151942424461045E-5 0.0 2.7498539783313216 0.002201024454604482 82-83 4.4835683334535747E-4 8.151942424461045E-5 0.0 3.0529635775288444 0.0022825438788490926 84-85 4.4835683334535747E-4 8.151942424461045E-5 0.0 3.3679750126660806 0.0023233035909713978 86-87 4.4835683334535747E-4 8.151942424461045E-5 0.0 3.7023676909174723 0.002404823015216008 88-89 4.4835683334535747E-4 8.151942424461045E-5 0.0 4.072160179147087 0.0025067222955217714 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8050 0.0 21.97036 1 GAGTACT 7380 0.0 21.229403 12-13 TACATGG 7925 0.0 21.142954 2 ACATGGG 8010 0.0 20.209488 3 GTACTTT 7800 0.0 19.752924 14-15 GGTATCA 13735 0.0 19.712044 1 GTATCAA 17190 0.0 19.308405 1 AGTACTT 7635 0.0 19.218468 12-13 AGAGTAC 11890 0.0 19.068836 10-11 CATGGGG 4785 0.0 17.90434 4 ACTTTTT 9240 0.0 16.957333 16-17 TATCAAC 19505 0.0 16.817017 2 TCAACGC 19650 0.0 16.644745 4 ATCAACG 19695 0.0 16.630749 3 CAACGCA 19755 0.0 16.508358 5 GTGTAGC 1505 0.0 16.382912 1 AACGCAG 20040 0.0 16.24864 6 GAGTACA 7055 0.0 16.130152 1 TACTTTT 9145 0.0 15.993729 14-15 AGTACAT 6240 0.0 15.777582 2 >>END_MODULE