FastQCFastQC Report
Thu 26 May 2016
SRR938892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453403
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79370.3235098351147366No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76670.3125047128417141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.28576634168948195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.1676854556711637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36620.14926206579188173No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24770.10096180692695005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT79500.021.13026212-13
GTATCAA181250.020.1315781
GTACTTT83150.019.945914-15
GTACATG81050.019.8650991
TACATGG79050.019.8090232
AGAGTAC128350.019.41029510-11
AGTACTT82400.019.1484312-13
GGTATCA144200.018.7303071
ACATGGG80500.018.3911883
GTACCTG20950.017.7352641
TCAACGC203800.017.6022684
ATCAACG204450.017.5463073
TATCAAC205000.017.5455272
CAACGCA205300.017.4967735
TATAGCG1650.00147448317.2551335
AACGCAG208950.017.2138426
TACCTGG19900.016.9299742
GTGTAGC15300.016.8123871
ACTTTTT98850.016.68161216-17
CATGGGG46250.016.2104994
Produced by FastQC (version 0.11.5)