##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2453403 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15029165612009 33.0 31.0 34.0 30.0 34.0 2 32.38522330004488 34.0 31.0 34.0 30.0 34.0 3 32.48431505137965 34.0 31.0 34.0 30.0 34.0 4 36.051534949618954 37.0 35.0 37.0 35.0 37.0 5 35.96712566178488 37.0 35.0 37.0 35.0 37.0 6 36.005870213739854 37.0 35.0 37.0 35.0 37.0 7 35.95075615379944 37.0 35.0 37.0 35.0 37.0 8 35.91806319630326 37.0 35.0 37.0 35.0 37.0 9 37.599183664485615 39.0 37.0 39.0 35.0 39.0 10-11 37.517251344357206 39.0 37.0 39.0 35.0 39.0 12-13 37.44295780187764 39.0 37.0 39.0 34.0 39.0 14-15 38.69920127268125 40.0 38.0 41.0 34.0 41.0 16-17 38.687775306380566 40.0 38.0 41.0 34.0 41.0 18-19 38.79204089177359 40.0 38.0 41.0 34.0 41.0 20-21 38.789776485966634 40.0 38.0 41.0 34.0 41.0 22-23 38.73161930591917 40.0 38.0 41.0 34.0 41.0 24-25 38.669369443177494 40.0 38.0 41.0 34.0 41.0 26-27 38.51305655858414 40.0 38.0 41.0 34.0 41.0 28-29 38.33394207963388 40.0 38.0 41.0 34.0 41.0 30-31 38.17617549990768 40.0 38.0 41.0 33.0 41.0 32-33 38.025872023471074 40.0 37.0 41.0 33.0 41.0 34-35 37.99621953670066 40.0 37.0 41.0 33.0 41.0 36-37 37.90154695335418 40.0 37.0 41.0 33.0 41.0 38-39 37.78109853945723 40.0 37.0 41.0 33.0 41.0 40-41 37.63471288654983 40.0 37.0 41.0 33.0 41.0 42-43 37.46840388635703 40.0 37.0 41.0 32.0 41.0 44-45 37.224845653160116 40.0 36.5 41.0 31.0 41.0 46-47 36.93515252080478 40.0 36.0 41.0 31.0 41.0 48-49 36.64858280518936 39.5 36.0 41.0 30.0 41.0 50-51 36.37769375842453 39.0 35.5 41.0 30.0 41.0 52-53 36.145704761916406 39.0 35.0 40.5 29.5 41.0 54-55 35.89089216080684 39.0 35.0 40.0 28.5 41.0 56-57 35.598189942704074 38.0 35.0 40.0 28.0 41.0 58-59 35.2807520819042 38.0 34.0 40.0 28.0 41.0 60-61 34.85035458911561 38.0 34.0 40.0 26.5 41.0 62-63 34.52930460262745 37.0 34.0 40.0 26.0 41.0 64-65 34.25130502408288 37.0 33.5 39.5 26.0 41.0 66-67 33.77408725757652 36.0 33.0 39.0 25.5 40.5 68-69 33.337282949437984 36.0 32.5 39.0 25.0 40.0 70-71 32.856766295631004 35.0 32.0 38.0 23.5 40.0 72-73 32.324374552407406 35.0 31.5 37.5 22.0 39.5 74-75 31.84504522901456 35.0 31.0 37.0 21.5 39.0 76-77 29.88287533682807 33.0 28.5 35.0 18.5 37.5 78-79 30.86223196922805 34.0 30.5 36.0 20.0 38.0 80-81 30.85206160585929 34.0 31.0 35.5 19.5 37.0 82-83 30.509483154622373 34.0 30.5 35.0 18.0 37.0 84-85 30.083370118973523 34.0 30.0 35.0 14.5 36.5 86-87 29.484899545651487 34.0 29.5 35.0 7.0 36.0 88-89 28.889202874537936 34.0 29.0 35.0 2.0 35.5 90-91 28.395649634405764 33.5 28.5 35.0 2.0 35.0 92-93 27.997183503892348 33.0 27.0 35.0 2.0 35.0 94-95 27.551293652123192 33.0 27.0 35.0 2.0 35.0 96-97 26.863543820562704 33.0 25.5 35.0 2.0 35.0 98-99 25.879877256202917 32.5 24.5 34.0 2.0 35.0 100-101 21.354579740874208 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16370409386705376 1101 2 0.15981023081734946 1101 3 0.16627440557715545 1101 4 0.1350521432903804 1101 5 0.19788108060394904 1101 6 0.20586370225501582 1101 7 0.1178007441293758 1101 8 0.18451286643558973 1101 9 0.2624504499076181 1101 10-11 0.2254811842908282 1101 12-13 0.26806267713699583 1101 14-15 0.24536174109892528 1101 16-17 0.2741366999154451 1101 18-19 0.38851265791358003 1101 20-21 0.3495518766730541 1101 22-23 0.3062292324626128 1101 24-25 0.28364103328846824 1101 26-27 0.2788094070244185 1101 28-29 0.31726473047135073 1101 30-31 0.30147086714509186 1101 32-33 0.34614398800053436 1101 34-35 0.3854352618516046 1101 36-37 0.3860467601619959 1101 38-39 0.46154277574449765 1101 40-41 0.4831718719518321 1101 42-43 0.4814588555492634 1101 44-45 0.5155369061457193 1101 46-47 0.5465649165069095 1101 48-49 0.27992914162197735 1101 50-51 0.4539822173471677 1101 52-53 0.5295906445084313 1101 54-55 0.5995772264177859 1101 56-57 0.37003737728672803 1101 58-59 0.551645259046289 1101 60-61 0.6371055627052229 1101 62-63 0.410223481751423 1101 64-65 0.5365141113906873 1101 66-67 0.5433526290237651 1101 68-69 0.4103906008106648 1101 70-71 0.4730182382532533 1101 72-73 0.4863246382605624 1101 74-75 0.4220779524611551 1101 76-77 0.32502541500624105 1101 78-79 0.5333290830544435 1101 80-81 0.6117383920464334 1101 82-83 0.6642767016190803 1101 84-85 0.5930533748251143 1101 86-87 0.7562200521657836 1101 88-89 0.8024081889947965 1101 90-91 0.8307748817075549 1101 92-93 0.8705129309803787 1101 94-95 0.7573050199372062 1101 96-97 0.7639547932688693 1101 98-99 0.7976700866966908 1101 100-101 1.1702810363807217 1102 1 0.08378348588679785 1102 2 0.08610295023917303 1102 3 0.03091788319384392 1102 4 0.07469413123305912 1102 5 0.0679628972582691 1102 6 0.1331944515780421 1102 7 0.12674737729611962 1102 8 0.10465703467051668 1102 9 0.14265471329200352 1102 10-11 0.1610431653765403 1102 12-13 0.12883810211459945 1102 14-15 0.15048963744123256 1102 16-17 0.10783969309551367 1102 18-19 0.11598077933713569 1102 20-21 0.12364187374023317 1102 22-23 0.08055999305884143 1102 24-25 0.15330556228038716 1102 26-27 0.10522579175648872 1102 28-29 0.1263530302943252 1102 30-31 0.14229709671665347 1102 32-33 0.024666521265892527 1102 34-35 0.14307648428972186 1102 36-37 0.13287581233029044 1102 38-39 0.15055502972237633 1102 40-41 0.18790649435570828 1102 42-43 0.057754232227431146 1102 44-45 0.1202997294653727 1102 46-47 0.27226618613861575 1102 48-49 0.11960174616800856 1102 50-51 0.23857383462495108 1102 52-53 0.3131773357518881 1102 54-55 0.2563916753072917 1102 56-57 0.25199874024271907 1102 58-59 0.20963069012395152 1102 60-61 0.06120188529506976 1102 62-63 0.10570485760538872 1102 64-65 0.177566556683864 1102 66-67 0.3197719752098678 1102 68-69 0.30169172771997665 1102 70-71 0.18035044056762928 1102 72-73 0.31949931161933876 1102 74-75 0.24253635691560405 1102 76-77 0.011509428254225185 1102 78-79 0.22196896326496685 1102 80-81 0.38943545802387547 1102 82-83 0.4030471989518922 1102 84-85 0.2577214029852293 1102 86-87 0.4085364907766298 1102 88-89 0.43425194375347687 1102 90-91 0.6205286444093723 1102 92-93 0.5902644515662452 1102 94-95 0.5674178397049019 1102 96-97 0.4808627811603685 1102 98-99 0.719885214949123 1102 100-101 1.0476936596188118 1103 1 0.03925198697737642 1103 2 0.07034163070616728 1103 3 0.0972595223803907 1103 4 0.09109695708450971 1103 5 0.006686865614206283 1103 6 0.06561367721078426 1103 7 -0.0034715998648593427 1103 8 0.015754144793795888 1103 9 0.03621337736136354 1103 10-11 0.11794225063007957 1103 12-13 0.05124664134811496 1103 14-15 0.1282426926105984 1103 16-17 0.10124383688247462 1103 18-19 0.007700440717798074 1103 20-21 0.12101397229866961 1103 22-23 0.07378209017559811 1103 24-25 0.09885229597986012 1103 26-27 0.01603635522720026 1103 28-29 0.15343527079544117 1103 30-31 0.1381346484047441 1103 32-33 0.18908672187362896 1103 34-35 0.18425986465550892 1103 36-37 0.04712117470703703 1103 38-39 0.17984824628556595 1103 40-41 0.20328079681608813 1103 42-43 0.2631522159777262 1103 44-45 0.3212489868886763 1103 46-47 0.2644532230187835 1103 48-49 0.20969711784857026 1103 50-51 0.24577708019561584 1103 52-53 0.21518106694748695 1103 54-55 0.24684125446876237 1103 56-57 0.14051964552239582 1103 58-59 0.13066371587930092 1103 60-61 0.10679718865215904 1103 62-63 -6.883026692889871E-4 1103 64-65 -0.05017687230889578 1103 66-67 -0.004066754184968602 1103 68-69 0.09456604674632985 1103 70-71 -0.01753500631888727 1103 72-73 -0.08760754682106864 1103 74-75 -0.030545378905213028 1103 76-77 0.04693626546152174 1103 78-79 0.17733520301890948 1103 80-81 0.047685413743462135 1103 82-83 0.2404203326601113 1103 84-85 0.28629914837527437 1103 86-87 0.26200387540981396 1103 88-89 0.41845928318034353 1103 90-91 0.4080403323786932 1103 92-93 0.5492917725359234 1103 94-95 0.38427512854733337 1103 96-97 0.18904134199055278 1103 98-99 0.45255106770390086 1103 100-101 0.8893229961218303 1104 1 0.03295828856706606 1104 2 0.08864403207098093 1104 3 0.054510794513774385 1104 4 0.0901825706726882 1104 5 0.09618078047105172 1104 6 0.017089283940165956 1104 7 0.02460525225523469 1104 8 0.07931921036568212 1104 9 0.15677549417127778 1104 10-11 0.08200400159594778 1104 12-13 0.1268616556803579 1104 14-15 0.1297133158445689 1104 16-17 0.09364360375867165 1104 18-19 0.05740611028932108 1104 20-21 0.09395636577578159 1104 22-23 0.1384803865001416 1104 24-25 0.0018115617651517368 1104 26-27 0.0972980187984902 1104 28-29 0.10865073584324136 1104 30-31 0.0323442561902354 1104 32-33 0.07625949098103746 1104 34-35 0.17498501602582905 1104 36-37 0.10307992317508052 1104 38-39 0.23641584010903927 1104 40-41 0.2960017262621406 1104 42-43 0.12751220722395118 1104 44-45 0.06430103694837896 1104 46-47 0.10228974540148528 1104 48-49 0.11131728173310051 1104 50-51 -0.058275458908717326 1104 52-53 -0.11491368030519311 1104 54-55 0.02129469385898375 1104 56-57 -0.0657113521053887 1104 58-59 -0.039446692253768845 1104 60-61 0.054912827934415986 1104 62-63 0.12916673576983584 1104 64-65 -0.033505335475858544 1104 66-67 0.19565331115698115 1104 68-69 0.12481103251786863 1104 70-71 -0.04916481331178346 1104 72-73 -0.1435577061408182 1104 74-75 0.0758101971609122 1104 76-77 -0.11266469344530705 1104 78-79 0.08329342361221492 1104 80-81 0.1205267594697439 1104 82-83 0.17573885675097145 1104 84-85 0.18251700639558166 1104 86-87 0.07389410180008227 1104 88-89 0.14880440410571083 1104 90-91 0.32668884063152603 1104 92-93 0.17342715397552766 1104 94-95 0.35721591155914467 1104 96-97 0.43768993792427224 1104 98-99 0.4632850873698331 1104 100-101 1.2321994696752157 1105 1 -0.054344006741963824 1105 2 -0.07979247307120119 1105 3 0.005529104868330137 1105 4 -0.010360829651808956 1105 5 -0.008939724616936928 1105 6 -0.004892713449720532 1105 7 -0.007380990917070562 1105 8 0.05707099379664271 1105 9 -0.04936647965478613 1105 10-11 -0.006927632509452053 1105 12-13 0.056411667847612534 1105 14-15 0.05280535747469628 1105 16-17 0.13757478302166248 1105 18-19 0.0417932264071581 1105 20-21 0.05589854817833384 1105 22-23 -0.047262476740797865 1105 24-25 0.03808159352670515 1105 26-27 0.07220347403944771 1105 28-29 0.15154703863421304 1105 30-31 0.15657553291442383 1105 32-33 0.029913511972758045 1105 34-35 0.07306054872371703 1105 36-37 0.1326485513298863 1105 38-39 0.14724411606976417 1105 40-41 0.15645598451093434 1105 42-43 0.3009942080449619 1105 44-45 0.20256063918208156 1105 46-47 0.1559966935109074 1105 48-49 0.16007128623787992 1105 50-51 0.23247644223581432 1105 52-53 0.2587120929489046 1105 54-55 0.24228687672781035 1105 56-57 0.20086448684133273 1105 58-59 0.0955820332003583 1105 60-61 0.1155368998843258 1105 62-63 0.1460859939385628 1105 64-65 -0.018228348760239044 1105 66-67 0.19899811848414828 1105 68-69 0.17178860025287435 1105 70-71 0.1279810883468926 1105 72-73 0.13584697639588939 1105 74-75 0.12559511916115795 1105 76-77 0.10961714803128686 1105 78-79 0.3981492456039639 1105 80-81 0.22854461955669692 1105 82-83 0.27214477466432285 1105 84-85 0.21523715584430647 1105 86-87 0.23097309499539165 1105 88-89 0.5111624022149037 1105 90-91 0.8634496095316351 1105 92-93 0.7339869145507016 1105 94-95 0.8621851309562985 1105 96-97 0.5765743546359374 1105 98-99 0.8222695975661303 1105 100-101 1.293620175685664 1106 1 -0.07039627686905447 1106 2 -0.14834792378426442 1106 3 -0.15823240268635885 1106 4 -0.12817049194988783 1106 5 -0.023089155143047435 1106 6 -0.08669070136952683 1106 7 -0.01600362580302317 1106 8 0.021662192470181196 1106 9 -0.04366657332403179 1106 10-11 0.023078995649129297 1106 12-13 0.07147514004500266 1106 14-15 0.03378548781240909 1106 16-17 -0.07752842712231711 1106 18-19 -0.12651893919207424 1106 20-21 -0.20808190564383722 1106 22-23 -0.06839739731830718 1106 24-25 -0.057200339194736216 1106 26-27 -0.09826878570444819 1106 28-29 -0.05666171528919506 1106 30-31 0.015541564290693088 1106 32-33 -0.03936581702608777 1106 34-35 0.05941046933394745 1106 36-37 -0.06793773410053205 1106 38-39 0.08738967373938777 1106 40-41 0.03174859325287116 1106 42-43 0.11586662115976054 1106 44-45 0.18058798343672322 1106 46-47 0.06866390035182945 1106 48-49 0.08367056237682391 1106 50-51 0.0978899425270825 1106 52-53 0.21734716424467848 1106 54-55 0.2504065432391016 1106 56-57 0.18513182555642516 1106 58-59 0.2615895873719509 1106 60-61 0.17055328597387387 1106 62-63 0.0489934611930849 1106 64-65 0.07301924362315759 1106 66-67 0.1426201005447112 1106 68-69 0.2087192254115635 1106 70-71 0.20376593432315815 1106 72-73 0.21511573961388564 1106 74-75 0.437765627507158 1106 76-77 0.17612696435481823 1106 78-79 0.4420589014599088 1106 80-81 0.5575044533979963 1106 82-83 0.6546474867318075 1106 84-85 0.4474592046687569 1106 86-87 0.4447325011068699 1106 88-89 0.5758303552404129 1106 90-91 0.8818227686587683 1106 92-93 0.8165185128498393 1106 94-95 0.8602326835156333 1106 96-97 0.8776982478040765 1106 98-99 0.8261730283271937 1106 100-101 1.2553843303697967 1107 1 -0.11501808218449838 1107 2 -0.07545701216502465 1107 3 -0.05852150586563454 1107 4 -0.12030976963829687 1107 5 -0.13191950616993608 1107 6 -0.039835213473573106 1107 7 -0.10660880253536931 1107 8 -0.10838565376263887 1107 9 -0.030465167637075297 1107 10-11 0.0016945450739243029 1107 12-13 -0.043033324463465306 1107 14-15 0.04521235964391224 1107 16-17 0.04344854934598885 1107 18-19 -0.12096297352050556 1107 20-21 0.01889421930916768 1107 22-23 0.027514900039079748 1107 24-25 0.014236558542741307 1107 26-27 -0.05541516221498455 1107 28-29 -0.03871912046798798 1107 30-31 -0.11635875691597874 1107 32-33 0.10569968613189928 1107 34-35 -0.055138768336675525 1107 36-37 0.0049635639452745295 1107 38-39 -0.03764968309187111 1107 40-41 -0.16706718244152086 1107 42-43 -0.08834520357230247 1107 44-45 -0.14806293134135728 1107 46-47 -0.04197686491320951 1107 48-49 -0.09892813871898909 1107 50-51 -0.0038104270923540184 1107 52-53 0.0035854923905205283 1107 54-55 -0.05898003955788056 1107 56-57 0.0889087937842632 1107 58-59 0.06737517032515683 1107 60-61 -0.12482904611366052 1107 62-63 -0.12992672813172845 1107 64-65 -0.22934662984097542 1107 66-67 0.06067162408756843 1107 68-69 -0.06708761108891537 1107 70-71 -0.09106411455313435 1107 72-73 -0.14509420354404057 1107 74-75 -0.0743197952719079 1107 76-77 -0.2706124702458901 1107 78-79 -0.12185418904277512 1107 80-81 0.0019155258437493217 1107 82-83 -9.850934366895103E-5 1107 84-85 -0.0246375315898284 1107 86-87 0.13852905762018963 1107 88-89 0.18837664350924754 1107 90-91 0.5271834695108026 1107 92-93 0.6030369311601653 1107 94-95 0.5626370125095548 1107 96-97 0.6513282266363696 1107 98-99 0.4223050715690633 1107 100-101 1.00978886194196 1108 1 -0.032989420333883857 1108 2 -0.10809990059426866 1108 3 0.010700009646676278 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-2.07607242093982 2316 90-91 -2.165401423074094 2316 92-93 -2.6417309136076774 2316 94-95 -2.8390261891536426 2316 96-97 -3.0089281836200463 2316 98-99 -3.322601373337381 2316 100-101 -3.7281182759651763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 13.0 5 44.0 6 118.0 7 294.0 8 690.0 9 1292.0 10 2116.0 11 3289.0 12 4656.0 13 6362.0 14 7838.0 15 10683.0 16 13857.0 17 18969.0 18 19550.0 19 14297.0 20 13815.0 21 14669.0 22 15772.0 23 17672.0 24 20059.0 25 23848.0 26 28828.0 27 35255.0 28 42319.0 29 51455.0 30 62542.0 31 75250.0 32 93231.0 33 118685.0 34 158626.0 35 227633.0 36 355608.0 37 551226.0 38 412576.0 39 30254.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.1865433412458 19.695956454121305 15.418024064827698 22.69947613980519 2 15.651933253525819 20.823892365013005 39.86287617647814 23.66129820498304 3 19.800456753334043 24.31801868669762 29.879396087801315 26.002128472167023 4 13.46651976866418 17.4070872172244 36.70501747980254 32.42137553430888 5 13.966034821135608 37.106784680004644 34.14085379647386 14.786326702385876 6 31.791393423746527 36.31678122183759 17.209076535734244 14.682748818681643 7 28.228913064832806 32.54646709081223 20.736014425677315 18.488605418677647 8 25.647396697566606 35.647017632243866 19.34264366677631 19.36294200341322 9 25.49507765336555 17.773843106900905 20.617199864840792 36.11387937489275 10-11 25.04478473369438 26.72359575658789 26.928352170434287 21.303267339283437 12-13 26.060965116615577 23.958273467506153 27.527132721367014 22.453628694511256 14-15 22.735824042883046 25.494477902950248 26.177823528896266 25.59187452527044 16-17 22.15083198856742 28.63038903306881 26.718525242536806 22.50025373582697 18-19 22.03125617764387 27.526113728563956 28.673418105382602 21.769211988409566 20-21 22.972988946373672 26.375711613624013 28.68091789241311 21.970381547589206 22-23 22.49222814189108 26.275116644106166 28.797551808651082 22.43510340535167 24-25 22.647767203349797 26.46032877598992 28.74160910376322 22.15029491689706 26-27 22.400722588176507 26.49195831259683 28.951052884503685 22.15626621472298 28-29 22.116423596123425 27.061717948498476 28.738531745497987 22.08332670988011 30-31 22.475475900208814 26.529334968612982 28.58309458331958 22.412094547858626 32-33 22.313965540924176 26.614380107956176 28.62911229830566 22.44254205281399 34-35 22.83991566624785 26.609520257525688 28.4816561860886 22.06890789013786 36-37 22.62612382725752 26.378308707235863 28.747904797931213 22.2476626675754 38-39 22.325870824224136 26.75649696486945 28.736170089604734 22.181462121301678 40-41 22.591774133820884 26.67138539134199 28.533209638345014 22.203630836492113 42-43 22.301889668715937 26.737298101038032 28.230454721277837 22.73035750896819 44-45 22.64078037137095 26.643782740332178 28.205454806317448 22.509982081979427 46-47 22.872837131536397 26.914192851788766 27.862429113592295 22.350540903082543 48-49 22.74812137950824 26.952558820508386 27.875157536736832 22.424162263246536 50-51 22.3812698233484 27.14207309604655 28.053608305411064 22.423048775193983 52-53 22.76558315123932 27.316669947823492 27.425253820917312 22.492493080019873 54-55 22.630179387569022 26.97180202355667 27.569828519815132 22.828190069059183 56-57 22.686325891017496 27.165675594266414 27.55397299179955 22.594025522916535 58-59 23.059125151957645 26.946611790384555 27.52501378190856 22.46924927574924 60-61 22.710838757222795 26.984882674423154 28.0009855935409 22.303292974813154 62-63 22.4123187262753 26.97975016742052 28.043272955971766 22.564658150332413 64-65 22.36661609054383 27.263396983163148 27.741122260049583 22.628864666243445 66-67 22.531325833298858 27.12896748202929 27.41728559512001 22.922421089551847 68-69 22.334603292873158 27.090654078754884 27.755232723788083 22.819509904583878 70-71 22.649091893993774 26.86564335333412 27.589372801777774 22.895891950894327 72-73 22.570486789165905 26.721048274580244 27.77313796388119 22.93532697237266 74-75 22.291792257529643 27.479341958903614 27.290176134943994 22.938689648622752 76-77 22.42972823018332 26.990983314019296 27.43842005672389 23.140868399073497 78-79 22.496261312357824 27.019533292763796 27.532800386076307 22.951405008802066 80-81 22.545725264051605 27.020265321270088 27.422950897182403 23.011058517495904 82-83 22.507859491489985 26.95205394303341 27.612381659270817 22.92770490620579 84-85 22.406755025570604 27.06644607510466 27.449302051069473 23.07749684825526 86-87 22.255150181086766 27.085559287164116 27.569396183554325 23.089894348194797 88-89 22.309577129194828 27.012085532160533 27.3063633274516 23.371974011193036 90-91 22.379745095962214 26.80253014526741 27.43590488152316 23.38181987724722 92-93 22.50162325553527 26.966095663859548 27.39863772890145 23.133643351703736 94-95 22.353583981107057 26.774239698899855 27.479158540199062 23.393017779794025 96-97 22.031683106168018 26.819936255535453 27.684393366809466 23.463987271487056 98-99 22.054754151682378 26.919751871176484 27.293987168027428 23.731506809113707 100-101 22.227471181278688 26.165481201265266 27.950476003369207 23.65657161408684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 567.0 1 405.0 2 288.0 3 330.0 4 303.5 5 314.0 6 543.5 7 1215.5 8 1998.0 9 2495.0 10 2369.5 11 2034.0 12 2086.0 13 2288.0 14 2483.5 15 2598.5 16 2717.0 17 2885.0 18 3082.5 19 3435.0 20 3959.0 21 4660.0 22 5708.0 23 7058.5 24 8467.0 25 10324.5 26 13472.0 27 17154.5 28 20286.5 29 23791.0 30 27310.0 31 31427.5 32 36006.5 33 39969.0 34 44624.0 35 49277.5 36 54574.5 37 59505.0 38 63133.5 39 66296.0 40 69354.0 41 72671.0 42 75226.0 43 78105.5 44 82123.0 45 85528.0 46 87892.0 47 95290.0 48 108703.5 49 108424.0 50 97020.5 51 92317.0 52 90297.0 53 88444.5 54 85802.5 55 79397.0 56 71937.5 57 64795.5 58 58592.0 59 52830.0 60 44982.0 61 36351.0 62 28395.0 63 21638.5 64 16181.0 65 11853.5 66 8466.0 67 6421.0 68 4589.5 69 3018.0 70 2100.0 71 1316.0 72 803.0 73 514.0 74 365.0 75 236.5 76 125.5 77 57.0 78 30.5 79 16.0 80 9.5 81 6.0 82 5.5 83 5.0 84 2.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4077194003594191 2 0.0 3 0.0 4 0.0 5 3.260776969784418E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013246906439749197 16-17 0.0026086215758275343 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022621640227879396 36-37 0.007295988469892634 38-39 0.012064874788202345 40-41 0.002302923734910245 42-43 4.075971212230522E-4 44-45 2.853179848561365E-4 46-47 2.4455827273383137E-4 48-49 2.853179848561365E-4 50-51 5.910158257734257E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.241784166726787E-4 60-61 0.002404823015216008 62-63 0.0 64-65 2.241784166726787E-4 66-67 0.0014877294924641408 68-69 0.0017934273333814297 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.126864603980675 78-79 8.151942424461045E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.147162940617583 88-89 0.44073476717848636 90-91 0.3393653631303133 92-93 0.0 94-95 0.0 96-97 5.502561136511205E-4 98-99 0.0 100-101 2.6493812879498395E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2453403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.92220220236957 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75123110912799 47.976984253158314 2 12.676053133529363 15.445061442577126 3 3.749697664134465 6.853195178764581 4 1.678992387384783 4.091516548819798 5 0.8753712840296859 2.6664773183902204 6 0.5162374481579577 1.8870193320668627 7 0.3326398596382143 1.4185606952612986 8 0.22687731931642988 1.1057492738021688 9 0.16968803826077106 0.9303992080421507 >10 0.9406020645626255 10.287381825936738 >50 0.057775293765155035 2.4080464079867485 >100 0.02242276065843999 2.3410831043601115 >500 0.001272808645868464 0.4894551958536288 >1k 9.378590022152428E-4 1.1761127185911637 >5k 2.0096978618898062E-4 0.9229574963890335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7937 0.3235098351147366 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7667 0.3125047128417141 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7011 0.28576634168948195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4114 0.1676854556711637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3662 0.14926206579188173 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2477 0.10096180692695005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2227913636691566E-4 2 0.0 0.0 0.0 0.0 1.2227913636691566E-4 3 0.0 0.0 0.0 0.0 1.2227913636691566E-4 4 0.0 0.0 0.0 0.0 1.2227913636691566E-4 5 0.0 0.0 0.0 0.0 1.2227913636691566E-4 6 0.0 0.0 0.0 0.0 1.2227913636691566E-4 7 0.0 0.0 0.0 0.0 1.2227913636691566E-4 8 0.0 0.0 0.0 0.0 1.2227913636691566E-4 9 0.0 0.0 0.0 4.075971212230522E-5 1.2227913636691566E-4 10-11 0.0 0.0 0.0 1.0189928030576305E-4 1.2227913636691566E-4 12-13 8.151942424461045E-5 0.0 0.0 1.4265899242806826E-4 3.4645755303959436E-4 14-15 8.151942424461045E-5 0.0 0.0 1.834187045503735E-4 4.483568333453574E-4 16-17 8.151942424461045E-5 0.0 0.0 2.241784166726787E-4 5.298762575899679E-4 18-19 8.151942424461045E-5 0.0 0.0 2.853179848561366E-4 5.910158257734257E-4 20-21 8.151942424461045E-5 8.151942424461045E-5 0.0 3.260776969784418E-4 6.725352500180362E-4 22-23 1.2227913636691566E-4 8.151942424461045E-5 0.0 5.094964015288153E-4 6.929151060791888E-4 24-25 1.2227913636691566E-4 1.0189928030576305E-4 0.0 5.298762575899679E-4 7.33674818201494E-4 26-27 1.2227913636691566E-4 1.2227913636691566E-4 0.0 8.967136666907149E-4 8.967136666907149E-4 28-29 1.2227913636691566E-4 1.2227913636691566E-4 0.0 0.0021602647424821767 9.374733788130201E-4 30-31 1.630388484892209E-4 1.2227913636691566E-4 0.0 0.005971297825917715 9.578532348741727E-4 32-33 1.834187045503735E-4 1.2227913636691566E-4 0.0 0.013858302121583775 9.782330909353253E-4 34-35 2.037985606115261E-4 1.2227913636691566E-4 0.0 0.025780517917358053 9.782330909353253E-4 36-37 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.0452840401678811 9.782330909353253E-4 38-39 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.07381583865349475 9.782330909353253E-4 40-41 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.10556765439677052 9.782330909353253E-4 42-43 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.13952049459465077 9.98612946996478E-4 44-45 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.18180869592154245 0.0010597525151799358 46-47 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.23516315908963997 0.0011208920833633935 48-49 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.29147270138660464 0.0011616517954856989 50-51 2.445582727338313E-4 1.2227913636691566E-4 0.0 0.3557711472595411 0.0012227913636691566 52-53 2.6493812879498395E-4 1.2227913636691566E-4 0.0 0.42294315283710016 0.001263551075791462 54-55 2.853179848561366E-4 1.2227913636691566E-4 0.0 0.49814482170275326 0.0013450705000360723 56-57 2.853179848561366E-4 1.2227913636691566E-4 0.0 0.5920551984325445 0.0013858302121583777 58-59 2.853179848561366E-4 1.2227913636691566E-4 0.0 0.6964000614656458 0.0014062100682195303 60-61 2.853179848561366E-4 1.2227913636691566E-4 0.0 0.8093248439004925 0.0015081093485252931 62-63 2.853179848561366E-4 1.2227913636691566E-4 0.0 0.9303608090476778 0.0015081093485252931 64-65 2.853179848561366E-4 1.630388484892209E-4 0.0 1.0541480547631188 0.0015488690606475985 66-67 2.853179848561366E-4 1.630388484892209E-4 0.0 1.1981928774033455 0.0016507683409533614 68-69 3.66837409100747E-4 1.630388484892209E-4 0.0 1.368120117241236 0.0016711481970145142 70-71 3.66837409100747E-4 1.630388484892209E-4 0.0 1.561484191549452 0.0016711481970145142 72-73 3.66837409100747E-4 1.630388484892209E-4 0.0 1.7609826025320747 0.001834187045503735 74-75 3.66837409100747E-4 1.630388484892209E-4 0.0 1.9713842365074143 0.0020176057500541087 76-77 3.66837409100747E-4 1.630388484892209E-4 0.0 2.198803050293816 0.0020379856061152613 78-79 3.8721726516189963E-4 1.630388484892209E-4 0.0 2.4680005690055813 0.0020787453182375664 80-81 4.075971212230522E-4 1.630388484892209E-4 0.0 2.765852165339327 0.002099125174298719 82-83 4.075971212230522E-4 1.630388484892209E-4 0.0 3.0700418969080907 0.002201024454604482 84-85 4.075971212230522E-4 1.630388484892209E-4 0.0 3.385359029886244 0.002241784166726787 86-87 4.075971212230522E-4 1.630388484892209E-4 0.0 3.720159305258859 0.00234368344703255 88-89 4.075971212230522E-4 1.630388484892209E-4 0.0 4.0908077474430415 0.0024455827273383132 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7950 0.0 21.130262 12-13 GTATCAA 18125 0.0 20.131578 1 GTACTTT 8315 0.0 19.9459 14-15 GTACATG 8105 0.0 19.865099 1 TACATGG 7905 0.0 19.809023 2 AGAGTAC 12835 0.0 19.410295 10-11 AGTACTT 8240 0.0 19.14843 12-13 GGTATCA 14420 0.0 18.730307 1 ACATGGG 8050 0.0 18.391188 3 GTACCTG 2095 0.0 17.735264 1 TCAACGC 20380 0.0 17.602268 4 ATCAACG 20445 0.0 17.546307 3 TATCAAC 20500 0.0 17.545527 2 CAACGCA 20530 0.0 17.496773 5 TATAGCG 165 0.001474483 17.255133 5 AACGCAG 20895 0.0 17.213842 6 TACCTGG 1990 0.0 16.929974 2 GTGTAGC 1530 0.0 16.812387 1 ACTTTTT 9885 0.0 16.681612 16-17 CATGGGG 4625 0.0 16.210499 4 >>END_MODULE