##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938891_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 355560 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.373320958488016 33.0 31.0 34.0 31.0 34.0 2 32.49713128585893 34.0 31.0 34.0 31.0 34.0 3 32.49977219034762 34.0 31.0 34.0 31.0 34.0 4 35.99439475756553 37.0 37.0 37.0 35.0 37.0 5 36.02634435819552 37.0 37.0 37.0 35.0 37.0 6 36.15368151648104 37.0 37.0 37.0 35.0 37.0 7 36.12776184047699 37.0 37.0 37.0 35.0 37.0 8 36.13508268646642 37.0 37.0 37.0 35.0 37.0 9 37.84154854314321 39.0 38.0 39.0 37.0 39.0 10-11 37.78832405219934 39.0 38.0 39.0 35.0 39.0 12-13 37.7406823039712 39.0 38.0 39.0 35.0 39.0 14-15 39.03285240184498 40.5 39.0 41.0 36.0 41.0 16-17 38.919566317921024 40.0 38.5 41.0 36.0 41.0 18-19 38.769619754753066 40.0 38.0 41.0 35.0 41.0 20-21 38.83553127460907 40.0 39.0 41.0 35.0 41.0 22-23 38.78668579142761 40.0 38.5 41.0 35.0 41.0 24-25 38.70842614467319 40.5 38.0 41.0 35.0 41.0 26-27 38.546675666554165 40.0 38.0 41.0 35.0 41.0 28-29 38.416755259309255 40.0 38.0 41.0 35.0 41.0 30-31 38.21156766790415 40.0 37.5 41.0 35.0 41.0 32-33 37.95030655866802 40.0 36.5 41.0 35.0 41.0 34-35 37.65871582855215 40.0 36.0 41.0 34.5 41.0 36-37 37.38059258634267 40.0 35.0 41.0 34.0 41.0 38-39 37.153684328945886 39.5 35.0 41.0 33.5 41.0 40-41 37.061154235572054 40.0 35.0 41.0 33.0 41.0 42-43 36.86482450219373 40.0 35.0 41.0 33.0 41.0 44-45 36.67265299808752 40.0 35.0 41.0 33.0 41.0 46-47 36.37084880188998 40.0 35.0 41.0 31.5 41.0 48-49 36.07853104961188 40.0 35.0 41.0 31.0 41.0 50-51 35.00814208572393 38.5 34.0 40.0 28.0 40.5 52-53 35.17872792215097 38.5 35.0 40.0 29.0 40.5 54-55 35.71783805827427 39.0 35.0 41.0 30.0 41.0 56-57 35.67912026099674 39.0 35.0 41.0 29.5 41.0 58-59 35.54323039712004 39.0 35.0 41.0 28.5 41.0 60-61 35.25508071774102 39.0 35.0 41.0 28.0 41.0 62-63 34.952055911801104 38.5 35.0 40.0 27.0 41.0 64-65 34.59560411744853 38.0 35.0 40.0 26.0 41.0 66-67 34.18574220947238 37.5 35.0 40.0 26.0 41.0 68-69 33.75701006862414 37.0 34.5 39.0 24.0 41.0 70-71 33.25888879514007 36.0 34.0 39.0 22.5 40.5 72-73 32.803436832039594 36.0 34.0 38.5 21.0 40.0 74-75 32.32536421419732 35.0 33.0 37.0 19.0 39.5 76-77 31.890156373045336 35.0 33.0 37.0 14.0 39.0 78-79 31.491646979412756 35.0 33.0 36.5 6.5 39.0 80-81 31.037616717291037 35.0 32.0 36.0 2.0 38.0 82-83 30.549171729103385 35.0 32.0 35.5 2.0 37.0 84-85 30.131616323545956 35.0 31.0 35.0 2.0 37.0 86-87 29.768849139385757 35.0 31.0 35.0 2.0 36.0 88-89 29.27676060299246 34.0 30.5 35.0 2.0 36.0 90-91 28.819064292946337 34.0 30.0 35.0 2.0 36.0 92-93 28.38060383620205 34.0 29.0 35.0 2.0 35.0 94-95 27.90286730790865 34.0 29.0 35.0 2.0 35.0 96-97 27.34226572167848 34.0 28.0 35.0 2.0 35.0 98-99 26.682011474856566 34.0 26.0 35.0 2.0 35.0 100-101 23.58564096073799 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2746110051364212 1101 2 0.15291017495496106 1101 3 0.26770864263063743 1101 4 0.4333793765634155 1101 5 0.22091467447588542 1101 6 0.2676819049635455 1101 7 0.15895715009556 1101 8 0.09055647547315715 1101 9 0.03151477207217823 1101 10-11 0.26518330883418884 1101 12-13 0.23948653702579037 1101 14-15 0.3093732024096951 1101 16-17 0.2993298064541179 1101 18-19 0.46314440241389576 1101 20-21 0.3351588125607776 1101 22-23 0.38920188947847123 1101 24-25 0.33052084844226925 1101 26-27 0.32452610534190285 1101 28-29 0.4451084479180949 1101 30-31 0.42945003091247 1101 32-33 0.2929439705522938 1101 34-35 0.2704666774268176 1101 36-37 0.10838392389898388 1101 38-39 0.2429648479948625 1101 40-41 0.34514305493696185 1101 42-43 0.21605097988749122 1101 44-45 0.48736516426321685 1101 46-47 0.5207829146546601 1101 48-49 0.5337054667436192 1101 50-51 0.5005327344093544 1101 52-53 0.670774004651129 1101 54-55 0.7619178245520501 1101 56-57 0.7053469817318287 1101 58-59 0.670240596592933 1101 60-61 0.7262340547845909 1101 62-63 0.7268363775825435 1101 64-65 0.4241484520913019 1101 66-67 0.6121516119054391 1101 68-69 0.8944553842520193 1101 70-71 0.7713256158421302 1101 72-73 0.6464254325937944 1101 74-75 0.667431997002133 1101 76-77 0.7302944255123798 1101 78-79 0.9005577199474359 1101 80-81 0.4579071167503379 1101 82-83 0.5830397822983819 1101 84-85 0.5891532897352612 1101 86-87 0.5954254450813252 1101 88-89 0.24080557988964912 1101 90-91 0.3940594437076008 1101 92-93 0.27769318993561853 1101 94-95 -0.06214592040951672 1101 96-97 -0.16470317955051428 1101 98-99 0.2743728372956866 1101 100-101 0.640129056314791 1102 1 0.19294640287932907 1102 2 0.17821639570916403 1102 3 0.3860853285651302 1102 4 0.23173817094821914 1102 5 0.3054350433170612 1102 6 0.30991628426440343 1102 7 0.35225470032953154 1102 8 0.29378039509881404 1102 9 0.3812945683837654 1102 10-11 0.3192880432134899 1102 12-13 0.4655479876175903 1102 14-15 0.4675757898030355 1102 16-17 0.7214757602113409 1102 18-19 0.6843114819624745 1102 20-21 0.7379423248096018 1102 22-23 0.7430502930017298 1102 24-25 0.6529386161273791 1102 26-27 0.5827751419507692 1102 28-29 0.6553513601118723 1102 30-31 0.7765718998912803 1102 32-33 0.6058740558812232 1102 34-35 0.5612559700224651 1102 36-37 0.6118160048238366 1102 38-39 0.3421167197262136 1102 40-41 0.7158163965279343 1102 42-43 0.803366903366701 1102 44-45 0.6394605399857625 1102 46-47 0.7194823366199756 1102 48-49 0.7497717342911017 1102 50-51 0.5635058282711825 1102 52-53 0.5592012686202921 1102 54-55 0.5633990384248833 1102 56-57 0.7033083772727124 1102 58-59 0.5873631537082673 1102 60-61 0.5738892295712716 1102 62-63 0.6811011037042078 1102 64-65 0.3991949013068279 1102 66-67 0.4110609155112712 1102 68-69 0.37295971951213147 1102 70-71 0.09689160813500308 1102 72-73 0.1529197210606199 1102 74-75 -0.10277341175095955 1102 76-77 -0.4553390041600629 1102 78-79 -0.5103630067247344 1102 80-81 -0.18975838008423196 1102 82-83 -0.060668929510040215 1102 84-85 -0.0712341311290352 1102 86-87 -0.2173842163555051 1102 88-89 -0.26790794887445557 1102 90-91 0.3391547506164905 1102 92-93 0.670687960098018 1102 94-95 0.2190954778839007 1102 96-97 0.4184409162380902 1102 98-99 0.5447760853000894 1102 100-101 1.5592988195958242 1103 1 0.10677693509221342 1103 2 0.24379762820215944 1103 3 0.3608402305259091 1103 4 0.27322976758687645 1103 5 0.260442046118186 1103 6 0.24566558398429095 1103 7 0.2582771092931111 1103 8 0.13576218781589944 1103 9 0.28479596894399606 1103 10-11 0.4252054101602667 1103 12-13 0.36441003243552217 1103 14-15 0.3952368542288056 1103 16-17 0.38581399550545825 1103 18-19 0.4907540589932893 1103 20-21 0.4310445657059674 1103 22-23 0.14842494286168062 1103 24-25 0.2443426777520301 1103 26-27 0.4061820046958573 1103 28-29 0.20594659820711314 1103 30-31 0.23674346851872485 1103 32-33 0.35496369173556985 1103 34-35 0.2764170344482437 1103 36-37 0.25336012247089457 1103 38-39 0.35852078135086884 1103 40-41 0.5434599539509009 1103 42-43 0.5690006568681056 1103 44-45 0.05637230469164933 1103 46-47 -0.005517663380018689 1103 48-49 -0.1481799463811626 1103 50-51 -0.06389263111257293 1103 52-53 -0.040676182360094515 1103 54-55 -0.07373331451629639 1103 56-57 -0.2835088496180518 1103 58-59 -0.1921361460116202 1103 60-61 -0.1505680533418996 1103 62-63 -0.5364584201053191 1103 64-65 -0.41388282978561364 1103 66-67 -0.010612753956515064 1103 68-69 0.0270038371591923 1103 70-71 -0.045632901668923864 1103 72-73 0.10321734010823747 1103 74-75 0.07868667228265025 1103 76-77 0.017577662506599978 1103 78-79 -0.22927056974994287 1103 80-81 -0.11582630725510157 1103 82-83 0.058711322590802695 1103 84-85 -0.021829369224274586 1103 86-87 0.21612418700583902 1103 88-89 0.08799191106952264 1103 90-91 0.08885713156886865 1103 92-93 0.35836303012602855 1103 94-95 0.15284897928446028 1103 96-97 0.31077284901120095 1103 98-99 0.5461766455241808 1103 100-101 0.10749559830730959 1104 1 0.046287305714457716 1104 2 0.13845930790294148 1104 3 0.261928295809831 1104 4 0.15303255575416586 1104 5 -0.036310759050124375 1104 6 0.1145027142121009 1104 7 0.13294558940192047 1104 8 0.19460271824772235 1104 9 0.03151477207217823 1104 10-11 0.17992319322725336 1104 12-13 0.2568275774882167 1104 14-15 0.30792811570449174 1104 16-17 0.18372287003793275 1104 18-19 0.12932937351216367 1104 20-21 0.1140605466648239 1104 22-23 0.20278570450737732 1104 24-25 0.1600006172283983 1104 26-27 0.057185064879469394 1104 28-29 0.28181365023023375 1104 30-31 0.3947679499876102 1104 32-33 0.5010364561014313 1104 34-35 0.4669984693343352 1104 36-37 0.6300602244770204 1104 38-39 0.6736006861451571 1104 40-41 0.5633511474225088 1104 42-43 0.35911456370251216 1104 44-45 0.4844749908528172 1104 46-47 0.6060430302615956 1104 48-49 0.7360176054719503 1104 50-51 0.6508217517503923 1104 52-53 0.7762653341308905 1104 54-55 0.7893744719509002 1104 56-57 0.7212429354890517 1104 58-59 0.5488533133559343 1104 60-61 0.5817253842643595 1104 62-63 1.0534259729582658 1104 64-65 1.019524174634654 1104 66-67 0.798567796876533 1104 68-69 0.8886750374312058 1104 70-71 1.0776839973450194 1104 72-73 0.9759052013799163 1104 74-75 0.8798597426668735 1104 76-77 1.1392539630846379 1104 78-79 0.9077831534734457 1104 80-81 0.6645545155942685 1104 82-83 0.8821727302752613 1104 84-85 1.1541821914693635 1104 86-87 1.273171109821206 1104 88-89 1.18444719838676 1104 90-91 0.9460825650948799 1104 92-93 0.7589070627679888 1104 94-95 0.7644436749662056 1104 96-97 0.4191118493512178 1104 98-99 0.11396821301822868 1104 100-101 0.03030246671941228 1105 1 0.1444037099400859 1105 2 -0.014620221696418412 1105 3 0.24025199523179452 1105 4 0.17806411511899256 1105 5 0.1994210860100054 1105 6 0.13883664551234887 1105 7 0.11415946223429074 1105 8 0.11510633926958747 1105 9 0.08408602979591961 1105 10-11 0.12829871644829893 1105 12-13 0.21221260173910395 1105 14-15 0.20669730047627155 1105 16-17 0.14137518549869554 1105 18-19 0.2994592652137058 1105 20-21 0.25364429525296117 1105 22-23 0.19558518626938337 1105 24-25 0.23981258985480025 1105 26-27 0.3094274408013362 1105 28-29 0.39847533383929346 1105 30-31 0.18138511828207982 1105 32-33 0.16067701154624814 1105 34-35 0.33557863012099887 1105 36-37 0.5669165795364819 1105 38-39 0.6358872451777771 1105 40-41 0.35862222575617864 1105 42-43 0.07189112339954562 1105 44-45 0.5582823790662275 1105 46-47 0.07293061248203969 1105 48-49 0.09198232542410523 1105 50-51 0.1038896542153438 1105 52-53 0.16875586632144746 1105 54-55 0.22036742922947639 1105 56-57 0.017809608799801424 1105 58-59 -0.23329571328680743 1105 60-61 -0.10124418586386952 1105 62-63 -0.2726653166570472 1105 64-65 -0.3029294829296276 1105 66-67 -0.051890644423046695 1105 68-69 -0.07783051037292665 1105 70-71 -0.04786413899143582 1105 72-73 -0.21833438402969563 1105 74-75 -0.3582639700432466 1105 76-77 -0.11949130301063704 1105 78-79 -0.11463230064920182 1105 80-81 -0.09949770684942294 1105 82-83 -0.04984175381217071 1105 84-85 -0.3574615531323211 1105 86-87 -0.20681360880213973 1105 88-89 0.009442215329155346 1105 90-91 0.12563874076426984 1105 92-93 0.25237925730655775 1105 94-95 -0.06846609847077545 1105 96-97 -0.36983905092118974 1105 98-99 -0.5200843416293033 1105 100-101 -0.012078438203847242 1106 1 0.26642896491823365 1106 2 0.3617760871913873 1106 3 0.3663083332599655 1106 4 0.27049571621984825 1106 5 0.3641288729616008 1106 6 0.33311380270839663 1106 7 0.2641594622342893 1106 8 0.2622327760511922 1106 9 0.30285727797464546 1106 10-11 0.2521938111544699 1106 12-13 0.40579362812732 1106 14-15 0.5367240124723835 1106 16-17 0.32719759119725467 1106 18-19 0.5446065859195528 1106 20-21 0.42487223761980175 1106 22-23 0.4759518691251401 1106 24-25 0.5381289246451573 1106 26-27 0.5051049541573391 1106 28-29 0.5119325137000672 1106 30-31 0.30277495153446665 1106 32-33 0.31581704716224834 1106 34-35 0.3076929250861866 1106 36-37 0.6670096667956642 1106 38-39 0.7896309719059573 1106 40-41 0.727842721142288 1106 42-43 0.35899651436603364 1106 44-45 0.37907321604210154 1106 46-47 0.2987076887326481 1106 48-49 0.48585070813415854 1106 50-51 0.4409748088211458 1106 52-53 0.5027165268362594 1106 54-55 0.4525715736361491 1106 56-57 0.42384409155842206 1106 58-59 0.40281000146144663 1106 60-61 0.7098089143465884 1106 62-63 0.6804429883453835 1106 64-65 0.7027286035201072 1106 66-67 0.7103483065240184 1106 68-69 0.6307942265543858 1106 70-71 0.9506181339565956 1106 72-73 0.979625790812463 1106 74-75 0.9780238719512511 1106 76-77 0.7260283667319491 1106 78-79 0.7215786431746665 1106 80-81 0.7398903456032251 1106 82-83 0.7616524990613875 1106 84-85 0.26971992655036914 1106 86-87 0.35442161865455546 1106 88-89 0.3068195829833549 1106 90-91 0.3649134695970382 1106 92-93 0.38504074346488437 1106 94-95 0.4233543645371576 1106 96-97 0.4698242160368835 1106 98-99 -0.22103545058186924 1106 100-101 -0.35594682091389984 1107 1 0.22026083868230728 1107 2 0.3421627048918623 1107 3 0.30114024738079337 1107 4 0.22915425772676912 1107 5 0.3134085049080042 1107 6 0.3099248120308076 1107 7 0.29682421868128017 1107 8 0.249052260291883 1107 9 0.3193147621346242 1107 10-11 0.24024417638308648 1107 12-13 0.435798874505295 1107 14-15 0.36496970438891907 1107 16-17 0.36895068857810287 1107 18-19 0.5299247989225009 1107 20-21 0.5155296491376191 1107 22-23 0.5695230382603 1107 24-25 0.5623268341653116 1107 26-27 0.41870518015515756 1107 28-29 0.48639072428161256 1107 30-31 0.7303386143134318 1107 32-33 0.6588655963369519 1107 34-35 0.7081126353246887 1107 36-37 0.7247278959413137 1107 38-39 0.6995708403429433 1107 40-41 0.5923812416636949 1107 42-43 0.4459895326476442 1107 44-45 0.4286797376934999 1107 46-47 0.643192938339169 1107 48-49 0.633047931589509 1107 50-51 0.9101382133168912 1107 52-53 0.8196179352869635 1107 54-55 0.9110888665051178 1107 56-57 1.0903197362575625 1107 58-59 1.2013410156266673 1107 60-61 1.2880001246456274 1107 62-63 0.8629685139921008 1107 64-65 0.5888055234926597 1107 66-67 0.764357750004379 1107 68-69 0.96169539286457 1107 70-71 0.9500733177816088 1107 72-73 1.1008534432600996 1107 74-75 1.015817971793858 1107 76-77 0.9571192097845405 1107 78-79 0.6120786633530386 1107 80-81 0.5224764063804948 1107 82-83 0.32268401768603283 1107 84-85 0.1045316622943453 1107 86-87 -0.3014765208991008 1107 88-89 -0.12745137226100312 1107 90-91 -0.16214573376064223 1107 92-93 -0.4070170474064163 1107 94-95 -0.28005174547535816 1107 96-97 0.24910505868589894 1107 98-99 0.513752071109888 1107 100-101 0.06791612755052512 1108 1 -0.37295977545602454 1108 2 -0.36990008884500014 1108 3 -0.27462052601466524 1108 4 -0.43056959918532556 1108 5 -0.5657119282194287 1108 6 -0.5451609449275594 1108 7 -0.501279631250128 1108 8 -0.5372006517108474 1108 9 -0.5802931828640396 1108 10-11 -0.5959368994547987 1108 12-13 -0.6286994493175229 1108 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2316 46-47 -1.2238200506479302 2316 48-49 -0.9196224491116993 2316 50-51 -1.0392358983929597 2316 52-53 -0.989400632352293 2316 54-55 -0.8783284151560196 2316 56-57 -0.733185086160411 2316 58-59 -0.575581301262055 2316 60-61 -0.5029904281273545 2316 62-63 -0.7189517888055832 2316 64-65 -0.7757411540171617 2316 66-67 -1.1639596099402851 2316 68-69 -1.3761284767743405 2316 70-71 -0.9944821761267093 2316 72-73 -1.1400850471596655 2316 74-75 -1.4150721044375842 2316 76-77 -1.3691597380238996 2316 78-79 -1.198076155644781 2316 80-81 -1.1870308633480953 2316 82-83 -1.2359602330341026 2316 84-85 -1.161617167632766 2316 86-87 -1.184488233028489 2316 88-89 -1.0343243629201808 2316 90-91 -0.9954930010571239 2316 92-93 -1.3106180901735485 2316 94-95 -0.910530643121529 2316 96-97 -1.156753286111286 2316 98-99 -1.2231126351748358 2316 100-101 -2.1496779076998678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2137.0 3 349.0 4 292.0 5 260.0 6 257.0 7 292.0 8 344.0 9 355.0 10 419.0 11 581.0 12 964.0 13 1721.0 14 1611.0 15 1763.0 16 2667.0 17 4289.0 18 3630.0 19 1712.0 20 1519.0 21 1376.0 22 1481.0 23 1599.0 24 1869.0 25 2199.0 26 2478.0 27 2943.0 28 3600.0 29 4443.0 30 5404.0 31 6785.0 32 9234.0 33 12831.0 34 20634.0 35 42254.0 36 43799.0 37 74229.0 38 80778.0 39 12462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.53358524617901 24.894253003214708 17.86250070497998 17.709661045626305 2 13.55737109760868 25.797645549029486 39.79517458566335 20.849808767698484 3 17.907704605200202 30.75734462163729 34.64708100896722 16.687869764195288 4 10.332947723069907 21.723086796085354 42.49819157230474 25.445773908539998 5 9.20545554351345 41.16936675721705 37.63987219727786 11.98530550199164 6 22.102144517160077 42.02938511836767 20.191717920870637 15.676752443601618 7 20.131788485670395 38.50227056592519 25.92074683893331 15.445194109471103 8 21.18123063850381 34.399804169281026 23.566932745090867 20.852032447124298 9 22.175795791233003 21.322674950267444 27.299022793970757 29.2025064645288 10-11 20.477032572652927 29.372927543327272 30.284280912929034 19.86575897109077 12-13 23.842918237578075 29.56811659445163 29.659135670474367 16.92982949749592 14-15 20.988852687790853 30.45464265014546 31.347806269730068 17.20869839233362 16-17 15.580905916678626 33.65295608844772 32.4477588423804 18.318379152493254 18-19 15.23228003038915 33.393877149047526 34.86029432454487 16.51354849601846 20-21 16.737644928606702 31.368452448785213 33.81505695847244 18.07884566413565 22-23 16.411612245989154 30.82434451764322 34.43347244539875 18.330570790968878 24-25 17.653995257895886 32.02524431686261 33.81176520252443 16.508995222717072 26-27 17.13138721052291 30.437694708433355 36.22512586572259 16.205792215321143 28-29 15.432196044440943 32.096142373110304 35.927034906626744 16.54462667582201 30-31 16.03364484929325 31.814024690524228 34.951274626504464 17.201055833678055 32-33 16.24158297714284 32.01606163525906 35.09163404909098 16.650721338507125 34-35 17.390023123343298 30.977243246291806 34.45082059669505 17.181913033669844 36-37 17.496078046521216 30.574857418810975 35.46801507498662 16.46104945968119 38-39 16.780027104861936 31.721723417471342 34.85755830368739 16.640691173979334 40-41 15.67709171388002 32.17085446995424 35.83458247587109 16.317471340294656 42-43 15.680582812124976 32.44970280534809 33.82117494246707 18.048539440059862 44-45 16.154757416718557 31.707733776126716 33.780221424930566 18.35728738222416 46-47 17.493385216205304 32.442231829464234 32.98991051334111 17.074472440989354 48-49 16.870413231076423 31.869897804281855 33.4982228366549 17.76146612798683 50-51 15.751964212084069 32.39382522889919 34.76231762905057 17.091892929966164 52-53 16.09837653580955 33.46324435840192 33.32542570572046 17.11295340006806 54-55 15.788121174468927 31.944622900590907 33.81871836325514 18.448537561685033 56-57 17.06203555214239 32.60536896076616 32.35055790210738 17.982037584984077 58-59 17.549391716504182 31.480135334079545 33.278520302229175 17.691952647187104 60-61 16.937255471857306 32.08618395711966 34.06441927696564 16.912141294057392 62-63 16.79799067907762 31.94314830318834 33.92267954634978 17.33618147138426 64-65 16.525776777316775 32.60981926741023 33.742540085127054 17.121863870145937 66-67 16.491305870655758 31.77142179238035 32.39806006236724 19.339212274596644 68-69 15.75290513311986 31.886616824700447 33.76847356742013 18.59200447475956 70-71 17.437926996865805 31.990531033323588 32.48855574246783 18.082986227342772 72-73 16.9743849753058 30.48946347842912 34.05511672016959 18.48103482609549 74-75 16.099601587998773 33.18471148672219 32.61018471572365 18.10550220955539 76-77 16.75646597368711 31.32838025205931 33.10982342293497 18.80533035131861 78-79 16.74628652419794 30.98878263496554 33.83900413063303 18.425926710203484 80-81 17.260477034171686 31.53790226375938 32.643311427502525 18.55830927456641 82-83 16.546657969565796 31.50002400173942 33.52802979604169 18.425288232653095 84-85 16.64313548314226 30.92110464787937 32.93499745863218 19.50076241034619 86-87 16.57070406149137 31.48453538379865 33.244194944808555 18.70056560990142 88-89 17.60607827716255 30.402540428421958 32.578961265244054 19.412420029171436 90-91 17.020729742286893 29.780981284060594 33.60890140415442 19.589387569498086 92-93 17.639199979663374 31.16757663660423 33.20010959182694 17.993113791905458 94-95 17.28829933279475 30.154457168360572 33.133717875564244 19.423525623280437 96-97 16.639551009916985 29.92325704230325 33.34251311296213 20.094678834817632 98-99 17.320671738648414 30.22301372881045 32.1109546614544 20.34535987108674 100-101 17.54356982396986 29.011720752127484 33.38967088421715 20.055038539685498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15457.0 1 8655.0 2 1712.0 3 1387.0 4 972.0 5 1002.5 6 1843.0 7 3283.0 8 4361.0 9 4158.0 10 2860.5 11 1729.0 12 1473.5 13 1375.5 14 1199.0 15 1078.0 16 1011.5 17 945.0 18 853.0 19 852.0 20 955.0 21 1073.5 22 1248.5 23 1373.5 24 1477.5 25 1859.5 26 2721.5 27 3625.0 28 3763.0 29 3937.5 30 5021.5 31 6213.0 32 7805.5 33 10196.5 34 12870.5 35 14431.0 36 16421.0 37 19046.5 38 18332.0 39 15985.5 40 14190.0 41 12451.0 42 10989.0 43 10249.5 44 9976.0 45 9629.0 46 9680.5 47 12476.5 48 23491.0 49 22450.0 50 9658.5 51 4493.0 52 2126.0 53 1339.5 54 942.0 55 702.0 56 539.0 57 428.0 58 397.0 59 374.0 60 352.0 61 332.5 62 285.0 63 212.0 64 166.0 65 136.5 66 110.0 67 100.0 68 83.5 69 63.0 70 50.5 71 45.0 72 40.5 73 33.0 74 30.0 75 23.5 76 17.0 77 15.0 78 11.0 79 9.5 80 8.5 81 5.0 82 2.5 83 2.5 84 2.5 85 2.0 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2643716953538081 2 0.06553043086961413 3 0.0748115648554393 4 0.07846776915288559 5 0.09140510743615704 6 0.03993700078749016 7 0.03993700078749016 8 0.042468219147260663 9 0.044718191022612215 10-11 0.04345258184272696 12-13 0.03937450781865227 14-15 0.03881201484981438 16-17 0.03965575430307121 18-19 0.04781190235122061 20-21 0.04767127910901114 22-23 0.059343008212397345 24-25 0.06538980762740466 26-27 0.062155473056586794 28-29 0.05962425469681629 30-31 0.0565305433682079 32-33 0.050343120710991104 34-35 0.2643716953538081 36-37 0.41160422994712564 38-39 0.3881201484981438 40-41 0.37040161997975024 42-43 0.39796377545280687 44-45 0.4062605467431657 46-47 0.40190122623467206 48-49 0.41751040611992346 50-51 0.4145573180335245 52-53 0.4138542018224772 54-55 0.40569805377432777 56-57 0.386995162560468 58-59 0.37279221509731125 60-61 0.3314489818877264 62-63 0.3966981662729216 64-65 0.4221509731128361 66-67 0.42974462819214754 68-69 0.44282258971762856 70-71 0.43916638542018227 72-73 0.43016649791877604 74-75 0.43016649791877604 76-77 0.4041511981100236 78-79 0.3872764090448869 80-81 0.39163572955338055 82-83 0.39908876139048266 84-85 0.3993700078749015 86-87 0.40190122623467206 88-89 0.40682303971200356 90-91 0.42285408932388346 92-93 0.42721340983237716 94-95 0.4348070649116886 96-97 0.42777590280121497 98-99 0.4284790190122624 100-101 0.4062605467431657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 355560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.078917763527954 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1425918009346 13.573236584542695 2 9.838288839423912 3.7540780740240747 3 4.134911626398573 2.36668916638542 4 2.308474726181877 1.76172797840027 5 1.7203001311968396 1.6410732365845428 6 1.2205728437283487 1.3972325345933174 7 0.8859471969574126 1.1832039599505006 8 0.7753880625617289 1.1834852064349195 9 0.6677771717499299 1.1466419169760378 >10 5.896487167769801 23.914669816627292 >50 0.8505682739507938 11.289515131060861 >100 0.49972728746849066 16.350545618179773 >500 0.028008314046906554 3.8595455056811794 >1k 0.028008314046906554 11.083642704466195 >5k 0.0014741217919424503 2.491000112498594 >10k+ 0.0014741217919424503 3.0037124535943303 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10680 3.0037124535943303 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8857 2.491000112498594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4844 1.3623579705253683 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4233 1.1905163685453932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3945 1.109517381032737 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3549 0.9981437732028351 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2687 0.7557093036337046 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2286 0.6429294633817078 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2234 0.6283046461919226 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1814 0.5101811227359658 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1624 0.4567442906963663 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1559 0.4384632692091349 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1310 0.36843289458881767 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1303 0.36646416919788505 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1275 0.35858926763415455 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1187 0.33383957700528744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1160 0.32624592192597596 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1131 0.31808977387782655 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1130 0.3178085273934076 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.31696478794015076 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1011 0.2843401957475532 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 971 0.2730903363707954 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 939 0.26409044886938915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.2469344133198335 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 870 0.24468444144448193 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.24299696253796826 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 802 0.2255596805039937 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 783 0.22021599730003374 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 776 0.21824727190910115 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 722 0.20305996175047814 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 721 0.20277871526605917 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 678 0.19068511643604455 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 649 0.18252896838789515 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 648 0.1822477219034762 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 600 0.16874789065136686 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 597 0.16790415119811 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 574 0.1614354820564743 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 564 0.15862301721228483 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 550 0.1546855664304196 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 537 0.15102936213297333 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 494 0.13893576330295873 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 467 0.13134210822364722 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 458 0.1288108898638767 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 450 0.12656091798852515 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 442 0.12431094611317359 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 438 0.1231859601754978 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 425 0.11952975587805152 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 401 0.11277984025199685 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 388 0.10912363595455057 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 380 0.106873664079199 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 377 0.10602992462594217 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 375 0.10546743165710429 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 375 0.10546743165710429 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 370 0.10406119923500956 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 369 0.10377995275059063 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.218697266284171E-4 0.0 22-23 0.0 0.0 0.0 0.0019687253909326133 0.0 24-25 0.0 0.0 0.0 0.0028124648441894477 0.0 26-27 0.0 0.0 0.0 0.007734278321520981 0.0 28-29 0.0 0.0 0.0 0.01659354258071774 0.0 30-31 0.0 0.0 0.0 0.04626504668691642 0.0 32-33 0.0 0.0 0.0 0.09323320958488018 0.0 34-35 0.0 0.0 0.0 0.1645291933850827 0.0 36-37 0.0 0.0 0.0 0.2611373607829902 0.0 38-39 0.0 0.0 0.0 0.3778546518168523 0.0 40-41 0.0 0.0 0.0 0.5010406119923501 0.0 42-43 0.0 0.0 0.0 0.6335077061536731 0.0 44-45 0.0 0.0 0.0 0.7943806952413095 0.0 46-47 0.0 0.0 0.0 0.9961750478119025 0.0 48-49 0.0 0.0 0.0 1.1846101923725953 0.0 50-51 0.0 0.0 0.0 1.3727640904488694 0.0 52-53 0.0 0.0 0.0 1.5621835977050287 0.0 54-55 0.0 0.0 0.0 1.7780402744965689 0.0 56-57 0.0 0.0 0.0 2.055489931375858 0.0 58-59 0.0 0.0 0.0 2.329142760715491 0.0 60-61 0.0 0.0 0.0 2.6733884576442795 0.0 62-63 0.0 0.0 0.0 3.0013218584767687 0.0 64-65 0.0 0.0 0.0 3.344583192710091 0.0 66-67 0.0 0.0 0.0 3.739875126560918 0.0 68-69 0.0 0.0 0.0 4.189588255146811 0.0 70-71 0.0 0.0 0.0 4.692597592530094 0.0 72-73 0.0 0.0 0.0 5.198560017999775 0.0 74-75 0.0 0.0 0.0 5.757818652266847 0.0 76-77 0.0 0.0 0.0 6.305405557430532 0.0 78-79 0.0 0.0 0.0 6.995303183710203 0.0 80-81 0.0 0.0 0.0 7.716137923275959 0.0 82-83 0.0 0.0 0.0 8.403785577680278 0.0 84-85 0.0 0.0 0.0 9.175104061199235 0.0 86-87 0.0 0.0 0.0 9.99198447519406 0.0 88-89 0.0 0.0 0.0 10.80506806164923 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGATC 25 0.00505755 56.88828 3 CGCAGAA 150 0.0 53.705143 9 ATGAGAG 45 9.709378E-4 42.139465 5 AGTACAC 80 2.2917266E-7 41.481033 2 GTTATGG 65 1.3417353E-4 36.466846 3 GCTTTGC 275 0.0 36.26799 1 ATGGGGA 175 0.0 35.216553 5 GTACCCA 55 0.0025788823 34.540943 1 ACCCATG 55 0.0026021528 34.47774 3 GCCTTAA 55 0.0026075465 34.46319 7 CTTTGCG 290 0.0 34.329132 2 GTGTTAT 70 2.0520536E-4 33.92414 1 CATTCCG 85 1.6558437E-5 33.449566 9 GGCATTC 85 1.6558437E-5 33.449566 7 AGGGGTG 85 1.6558437E-5 33.449566 8 CATATAG 100 1.2971595E-6 33.24566 1 CCTTTAG 90 2.4104447E-5 31.662533 1 TTGCGTT 320 0.0 31.110775 4 CATGGGG 345 0.0 30.230486 4 CTTTAGG 95 3.5311E-5 29.941198 2 >>END_MODULE