##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 355560 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.665516368545394 31.0 31.0 34.0 30.0 34.0 2 31.941028237147037 31.0 31.0 34.0 30.0 34.0 3 31.95767802902464 33.0 31.0 34.0 30.0 34.0 4 35.64881313983575 37.0 35.0 37.0 33.0 37.0 5 35.58241084486444 37.0 35.0 37.0 33.0 37.0 6 35.66814039824502 37.0 35.0 37.0 33.0 37.0 7 35.60702272471594 37.0 35.0 37.0 33.0 37.0 8 35.60755990550118 37.0 35.0 37.0 33.0 37.0 9 37.13083586455169 39.0 37.0 39.0 33.0 39.0 10-11 37.02206800539993 39.0 37.0 39.0 33.0 39.0 12-13 36.932431938350774 39.0 37.0 39.0 33.0 39.0 14-15 37.92059005512431 40.0 37.0 41.0 32.5 41.0 16-17 37.95336511418607 39.5 37.0 41.0 33.0 41.0 18-19 37.9344766002925 40.0 37.0 41.0 33.0 41.0 20-21 37.89857689278884 40.0 36.5 41.0 33.0 41.0 22-23 37.75666554168073 40.0 36.0 41.0 33.0 41.0 24-25 37.58402098098774 39.5 36.0 41.0 32.0 41.0 26-27 37.277332939588256 39.0 35.5 41.0 32.0 41.0 28-29 37.048647204409946 39.0 35.0 41.0 31.5 41.0 30-31 36.758562549218134 38.5 35.0 40.0 31.0 41.0 32-33 36.423247834402076 38.0 35.0 40.0 30.0 41.0 34-35 36.18032258971763 38.0 35.0 40.0 30.0 41.0 36-37 35.92184863314208 38.0 35.0 40.0 30.0 41.0 38-39 35.67826949038137 38.0 35.0 40.0 30.0 41.0 40-41 35.48295083811452 38.0 35.0 40.0 29.0 41.0 42-43 35.205783833952076 38.0 34.5 40.0 27.0 41.0 44-45 34.85032905838678 38.0 34.0 40.0 25.5 41.0 46-47 34.36814321070987 38.0 33.0 40.0 24.0 41.0 48-49 33.8589928563393 37.0 33.0 40.0 23.0 41.0 50-51 33.54907188660142 37.0 33.0 40.0 22.0 41.0 52-53 33.26199797502531 37.0 33.0 40.0 20.0 41.0 54-55 32.93191022612217 36.5 32.0 39.5 19.5 40.5 56-57 32.776186860164245 36.0 32.0 39.0 19.0 40.0 58-59 32.47429125885927 36.0 31.5 39.0 17.5 40.0 60-61 31.953325739678256 35.0 31.0 39.0 13.0 40.0 62-63 31.43034508943638 35.0 30.5 38.5 8.0 40.0 64-65 30.894650691866353 35.0 29.5 38.0 7.5 40.0 66-67 30.27621498481269 35.0 28.5 38.0 2.0 40.0 68-69 29.650452806839915 34.0 28.0 37.0 2.0 39.0 70-71 28.976984193947576 34.0 26.5 36.0 2.0 39.0 72-73 28.32231550230622 33.0 26.0 36.0 2.0 38.5 74-75 27.66997975025312 33.0 26.0 35.0 2.0 38.0 76-77 25.9291751040612 30.5 23.0 34.0 2.0 36.0 78-79 26.86759618629767 32.0 25.0 35.0 2.0 36.0 80-81 26.754296040049496 32.0 25.0 35.0 2.0 36.0 82-83 26.154492912588594 32.0 23.5 35.0 2.0 36.0 84-85 25.489153729328386 31.5 20.5 34.5 2.0 35.5 86-87 24.589502474969063 31.0 18.0 34.0 2.0 35.0 88-89 23.546381763977948 30.0 8.5 34.0 2.0 35.0 90-91 22.623257678029024 29.0 2.0 34.0 2.0 35.0 92-93 21.911531105861176 29.0 2.0 34.0 2.0 35.0 94-95 20.9822111598605 29.0 2.0 33.5 2.0 35.0 96-97 19.609763471706604 26.5 2.0 33.0 2.0 35.0 98-99 17.964422319721002 24.5 2.0 33.0 2.0 34.5 100-101 13.006233828327145 2.0 2.0 27.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09585651857943489 1101 2 -0.02569505749553258 1101 3 0.23209963735918393 1101 4 -0.13310894324369116 1101 5 0.17714028473168497 1101 6 0.08458636637031702 1101 7 0.2603933749405627 1101 8 0.014893251536427954 1101 9 0.4190690292214114 1101 10-11 0.5304404620792695 1101 12-13 0.21357407224962088 1101 14-15 0.285770831776162 1101 16-17 0.46303681481471415 1101 18-19 0.5131003020913028 1101 20-21 0.5455487827225127 1101 22-23 0.3604336413154954 1101 24-25 0.42755693918295634 1101 26-27 0.1568165418417422 1101 28-29 0.286287206438395 1101 30-31 0.4886380550801732 1101 32-33 0.6152460984326709 1101 34-35 0.663579045186296 1101 36-37 0.43531080520571663 1101 38-39 0.8264162516079523 1101 40-41 0.8453647927031369 1101 42-43 0.5760980458368223 1101 44-45 0.9618693741435393 1101 46-47 1.3448369697030316 1101 48-49 1.1461819049866762 1101 50-51 1.569194477312081 1101 52-53 1.6445652424617592 1101 54-55 1.3618465069408288 1101 56-57 1.3558532965759795 1101 58-59 1.9503357863881874 1101 60-61 2.087851226830743 1101 62-63 2.5405930246178627 1101 64-65 2.601126425330218 1101 66-67 2.477138500471984 1101 68-69 2.5687174458014717 1101 70-71 2.254988525669109 1101 72-73 2.190970438814155 1101 74-75 2.7049685811270727 1101 76-77 2.485506554079514 1101 78-79 2.8521322455578293 1101 80-81 3.234621626981916 1101 82-83 3.100566872567587 1101 84-85 3.1370597477977995 1101 86-87 3.1298310230005875 1101 88-89 3.0884653707930347 1101 90-91 3.059783547920876 1101 92-93 2.729319755606589 1101 94-95 2.53806385285748 1101 96-97 2.812912099833408 1101 98-99 2.6231285115135883 1101 100-101 1.9479830604213895 1102 1 -0.1518382240575029 1102 2 -0.15633777701108542 1102 3 -0.14895596407214384 1102 4 -0.031402364658497106 1102 5 0.01549701470690934 1102 6 0.030171970554185634 1102 7 -0.08819938586318443 1102 8 0.025232502843884674 1102 9 0.23168945311351052 1102 10-11 -0.011363831892651888 1102 12-13 -0.01511089884863992 1102 14-15 -0.09489769285912075 1102 16-17 0.22143208043541307 1102 18-19 0.3931064953200476 1102 20-21 0.14184488786977312 1102 22-23 0.11766767654808774 1102 24-25 0.035559966983669256 1102 26-27 0.3923914799094561 1102 28-29 0.3618015884917938 1102 30-31 0.3104278981850399 1102 32-33 0.20732736735642732 1102 34-35 0.25125624034181726 1102 36-37 0.03722382474879993 1102 38-39 0.0927594941072698 1102 40-41 0.10378586344357643 1102 42-43 0.5776893615538583 1102 44-45 0.19009845479313725 1102 46-47 -0.20116495707924997 1102 48-49 0.41375519152672524 1102 50-51 -0.1068910580581317 1102 52-53 -0.5513644299852558 1102 54-55 -0.2728648197863919 1102 56-57 -0.3119057868261663 1102 58-59 -0.47506161245573963 1102 60-61 -0.5485890429187776 1102 62-63 -0.5893701600779764 1102 64-65 -0.787163211333695 1102 66-67 -0.9576691653689196 1102 68-69 -0.589278700633983 1102 70-71 -0.3932618872128053 1102 72-73 -0.6370384381470338 1102 74-75 -0.8866567973480137 1102 76-77 -1.1003642826945033 1102 78-79 -0.7298850266689847 1102 80-81 -0.381819674972391 1102 82-83 -0.5816344761800032 1102 84-85 -0.6129144470824635 1102 86-87 0.11792626109582471 1102 88-89 -0.23983596419182263 1102 90-91 -0.23593452254430858 1102 92-93 -0.18207406492740574 1102 94-95 0.4151798726758109 1102 96-97 0.3996861157981755 1102 98-99 0.17193459464047933 1102 100-101 -0.026151607896805373 1103 1 0.12722340059235648 1103 2 0.2491944358740703 1103 3 0.4090972572163736 1103 4 0.21425589864682593 1103 5 0.08482474579935229 1103 6 0.2834983010863965 1103 7 0.46134543206398604 1103 8 0.3498473487822622 1103 9 0.2856110217409622 1103 10-11 0.2012412101241523 1103 12-13 0.1620949835042964 1103 14-15 0.2512332595218254 1103 16-17 0.06919118407687108 1103 18-19 0.4110686801939849 1103 20-21 0.42617612036276853 1103 22-23 0.6908994692651689 1103 24-25 0.41630226390243763 1103 26-27 0.4194397992371819 1103 28-29 0.6710627929735793 1103 30-31 0.5956169738152965 1103 32-33 0.6654331096533426 1103 34-35 0.8132135232549871 1103 36-37 0.652209819146556 1103 38-39 0.6802244801016712 1103 40-41 0.9447697570009979 1103 42-43 0.8299827789208081 1103 44-45 0.8786663819640026 1103 46-47 1.4875605331168273 1103 48-49 0.9645430066527751 1103 50-51 0.8974331716337431 1103 52-53 1.329272824916707 1103 54-55 1.6778529673284552 1103 56-57 1.4616761459469387 1103 58-59 1.0363354463677936 1103 60-61 1.436950172767503 1103 62-63 1.6147089715746858 1103 64-65 1.5976582172377327 1103 66-67 1.4822643080204365 1103 68-69 1.2317997307385689 1103 70-71 1.7847318102661838 1103 72-73 1.2679510576512847 1103 74-75 1.1615224743606696 1103 76-77 1.3457666696864479 1103 78-79 1.812324357084517 1103 80-81 1.7249905491172441 1103 82-83 1.472532190486664 1103 84-85 1.6685456369511513 1103 86-87 1.529831023000586 1103 88-89 1.6488019909902505 1103 90-91 1.987087186139167 1103 92-93 2.0212697726076065 1103 94-95 1.7875538222556422 1103 96-97 1.6267169281231055 1103 98-99 2.109714706685299 1103 100-101 1.465672621795072 1104 1 0.22302414863723996 1104 2 0.19106794828481455 1104 3 0.10493200730137886 1104 4 0.11833614346151222 1104 5 0.29563739455827687 1104 6 -0.04547143709789481 1104 7 0.13611591829316438 1104 8 -0.08048247100692407 1104 9 0.3034620928052263 1104 10-11 0.26598959502724995 1104 12-13 0.1442099103999155 1104 14-15 0.19328528264321676 1104 16-17 0.03962641019044355 1104 18-19 0.043447122900552415 1104 20-21 0.45306323358956035 1104 22-23 -0.04419063614114549 1104 24-25 -0.05654710705982069 1104 26-27 -0.039715250065775365 1104 28-29 0.16634500990659973 1104 30-31 0.24297331519577625 1104 32-33 0.2597547689529094 1104 34-35 -0.018501879669187815 1104 36-37 0.1535188976912636 1104 38-39 0.19924862155015433 1104 40-41 0.36559600657597 1104 42-43 -0.06841062468340198 1104 44-45 0.3896150388834201 1104 46-47 1.1035074899342447 1104 48-49 0.5912686101889904 1104 50-51 0.9131251131502296 1104 52-53 1.0983224678952794 1104 54-55 1.5843898595419859 1104 56-57 1.3255064757667299 1104 58-59 1.5009138210702702 1104 60-61 1.5661749262527067 1104 62-63 1.0593791517854996 1104 64-65 1.4941900091452531 1104 66-67 1.5913003501829657 1104 68-69 1.808601838865055 1104 70-71 1.7390925719118862 1104 72-73 1.5666929821667566 1104 74-75 1.3393616447108876 1104 76-77 1.4768360338483006 1104 78-79 1.7220744420896175 1104 80-81 1.7100551529934762 1104 82-83 1.508081323434638 1104 84-85 1.0301233316128275 1104 86-87 1.1659581906306435 1104 88-89 1.6086965846658678 1104 90-91 1.916719964105848 1104 92-93 2.5327879636990716 1104 94-95 3.03228350603667 1104 96-97 2.450195336827626 1104 98-99 1.7517412282765967 1104 100-101 1.4002951991497117 1105 1 0.20345734244495972 1105 2 0.2593026170775836 1105 3 0.21085520177350503 1105 4 0.22886036112146968 1105 5 0.008928870207732587 1105 6 0.022680180734809596 1105 7 0.24092622990036716 1105 8 -0.014689500440184133 1105 9 0.28054002782615584 1105 10-11 0.1778808314899436 1105 12-13 0.045952319542159614 1105 14-15 0.19404935147585434 1105 16-17 0.43756508536151983 1105 18-19 0.17132899121494916 1105 20-21 0.23385022448717763 1105 22-23 0.07369189658092523 1105 24-25 0.029402331515690605 1105 26-27 -0.07574275654375384 1105 28-29 0.5053258085246313 1105 30-31 0.6161544991702641 1105 32-33 0.733959140755438 1105 34-35 0.8739471270413333 1105 36-37 0.5538494405123444 1105 38-39 0.7413637634011963 1105 40-41 0.795750149157854 1105 42-43 0.4881470791236495 1105 44-45 0.8333147930525797 1105 46-47 0.7887775716563894 1105 48-49 0.796811431264004 1105 50-51 0.8396914542571281 1105 52-53 1.0628597079457762 1105 54-55 0.8930321424467778 1105 56-57 0.34916769429041494 1105 58-59 0.4204125254752924 1105 60-61 0.5512672789203066 1105 62-63 0.6553445361453427 1105 64-65 0.3249570678124485 1105 66-67 0.38495193479528567 1105 68-69 0.4911810694809624 1105 70-71 1.1241334329842374 1105 72-73 0.9020112334457409 1105 74-75 0.4815698036372069 1105 76-77 0.21157126738759757 1105 78-79 -0.1420874076213643 1105 80-81 0.30049764850872407 1105 82-83 0.5426471330153966 1105 84-85 0.8528593624413467 1105 86-87 0.5292563103569101 1105 88-89 0.35760861708896385 1105 90-91 0.5298187615279453 1105 92-93 0.8909452289970616 1105 94-95 0.898862138685665 1105 96-97 0.616845393302281 1105 98-99 0.9177268770706952 1105 100-101 0.6079309044184651 1106 1 -0.01399449501658978 1106 2 0.1793932759734851 1106 3 -0.09119271792861383 1106 4 -0.1715767442695011 1106 5 -0.019814856520454782 1106 6 -0.08145613139081576 1106 7 0.1646594337209919 1106 8 0.28298736535226965 1106 9 0.012205885038397923 1106 10-11 0.24210699305704253 1106 12-13 0.14598065044713593 1106 14-15 0.05228959755000062 1106 16-17 -0.11974833539289165 1106 18-19 -0.07905145236608035 1106 20-21 0.05154894554917888 1106 22-23 -0.03192571714742343 1106 24-25 0.34935943043102213 1106 26-27 0.22811834107645268 1106 28-29 0.43562368775403826 1106 30-31 0.30607679154520184 1106 32-33 0.3494844766790308 1106 34-35 0.200019654158055 1106 36-37 -0.27840327115999486 1106 38-39 0.2716495008141777 1106 40-41 0.21452949188086734 1106 42-43 7.988518288470914E-4 1106 44-45 0.3680201516408914 1106 46-47 0.4693749490240364 1106 48-49 0.4662828575226996 1106 50-51 0.8082036770189944 1106 52-53 0.4961120958363381 1106 54-55 0.676175253989598 1106 56-57 1.0883331467754402 1106 58-59 1.2246743030372187 1106 60-61 1.3130893608370897 1106 62-63 1.1467926501165238 1106 64-65 1.147658217237737 1106 66-67 1.252873255824909 1106 68-69 1.2738668392721202 1106 70-71 1.0284559196282395 1106 72-73 1.1268574371044764 1106 74-75 1.226539044368952 1106 76-77 1.2679497682779974 1106 78-79 1.4557999163222952 1106 80-81 0.9843733163086306 1106 82-83 0.8021096552753946 1106 84-85 1.1150659352112982 1106 86-87 1.3375775018738274 1106 88-89 1.3362916347350726 1106 90-91 1.5557698704805105 1106 92-93 1.5429971959713207 1106 94-95 1.6444303756939185 1106 96-97 1.6473051634172222 1106 98-99 0.97408918887254 1106 100-101 0.34299242295497123 1107 1 0.07771219209749702 1107 2 0.12980289702356984 1107 3 -0.010277426754637276 1107 4 0.04295975841422717 1107 5 -0.0529638940118744 1107 6 -0.03952546429368198 1107 7 -0.07388126606512202 1107 8 -0.08522765543145994 1107 9 0.027057170401000974 1107 10-11 0.009563306871157806 1107 12-13 0.09036904384476685 1107 14-15 -0.2074343622546735 1107 16-17 8.699300738399529E-4 1107 18-19 0.2712532411243771 1107 20-21 0.46687644060548195 1107 22-23 0.5511809457526127 1107 24-25 0.478884431439937 1107 26-27 0.24737254826802513 1107 28-29 0.03224179179374431 1107 30-31 0.296843166298018 1107 32-33 0.4095676115916618 1107 34-35 0.41518975787491286 1107 36-37 0.7200591365239362 1107 38-39 0.5466242778431081 1107 40-41 0.3563996238474516 1107 42-43 0.639392015394769 1107 44-45 0.6821974792166614 1107 46-47 0.3025360935415762 1107 48-49 0.12434411907482001 1107 50-51 -0.038400302156922805 1107 52-53 -0.02420349397761612 1107 54-55 0.17138490732508416 1107 56-57 0.3988622238164865 1107 58-59 0.33609526433509274 1107 60-61 0.8074878434231536 1107 62-63 0.422828809767843 1107 64-65 0.4903516269798551 1107 66-67 0.24684799249709144 1107 68-69 0.5494510032819697 1107 70-71 0.4389244615387291 1107 72-73 0.571810825053948 1107 74-75 0.3292833036207412 1107 76-77 0.586525982007366 1107 78-79 0.9531847986823614 1107 80-81 0.6855880545950832 1107 82-83 0.12926571484196359 1107 84-85 0.5780265066629298 1107 86-87 0.6907192751495863 1107 88-89 1.587012002788665 1107 90-91 1.0966270479291573 1107 92-93 1.2081693175258614 1107 94-95 1.395045816187885 1107 96-97 1.5518896906378536 1107 98-99 1.2908372416591725 1107 100-101 1.3111428729327752 1108 1 -0.08103355658144906 1108 2 0.15171901511086006 1108 3 -0.023922239534169165 1108 4 -0.03855196670895111 1108 5 0.054246508838843965 1108 6 -0.10352052910857168 1108 7 -0.014991845056492537 1108 8 0.13803265123185327 1108 9 0.12097179494543298 1108 10-11 0.13761431460673634 1108 12-13 0.20602932778025007 1108 14-15 0.16121909521587696 1108 16-17 0.11594172565327909 1108 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46-47 -0.5858908633568731 2316 48-49 -0.658206252201964 2316 50-51 -0.5307771575907836 2316 52-53 -0.7611898057589102 2316 54-55 -0.2814815622393425 2316 56-57 -0.7039932252498176 2316 58-59 -1.0620145302276214 2316 60-61 -0.9853153912830557 2316 62-63 -1.22341320037102 2316 64-65 -0.9894770612768546 2316 66-67 -0.7470109299262013 2316 68-69 -0.47632556181877206 2316 70-71 -0.2074315537531639 2316 72-73 -0.7530506586851153 2316 74-75 -0.5510340867253021 2316 76-77 -0.14143492182548556 2316 78-79 -0.580416320088208 2316 80-81 -0.6689983727114317 2316 82-83 -0.11871449386347166 2316 84-85 -0.8330380734560876 2316 86-87 -0.4079673854874777 2316 88-89 -0.3883020814079394 2316 90-91 -0.30130413856047156 2316 92-93 -0.011906229888879238 2316 94-95 0.0643130670676264 2316 96-97 0.7284722721705386 2316 98-99 0.4714404049221521 2316 100-101 0.8498628230745222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 15.0 5 82.0 6 196.0 7 272.0 8 291.0 9 512.0 10 775.0 11 1016.0 12 1449.0 13 1880.0 14 2425.0 15 3322.0 16 4213.0 17 5713.0 18 5403.0 19 4036.0 20 4109.0 21 4471.0 22 4888.0 23 5557.0 24 6302.0 25 7270.0 26 7946.0 27 9169.0 28 10950.0 29 12569.0 30 15015.0 31 17775.0 32 21540.0 33 26432.0 34 37090.0 35 43867.0 36 42293.0 37 36128.0 38 10369.0 39 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.01646596770007 26.47928493234169 18.886205378807546 16.6180437211507 2 14.304758690516369 27.451063111711104 39.00354370570368 19.24063449206885 3 18.74789065136686 31.146641916976037 34.54466194172573 15.560805489931376 4 10.871301608729892 23.246990662616717 41.1564855439307 24.725222184722693 5 9.039824502193722 42.116942288221395 37.16109798627517 11.682135223309709 6 21.85060186747666 43.34795815052312 19.295477556530543 15.505962425469683 7 20.15946675666554 39.11604229947125 25.3133085836427 15.411182360220495 8 21.658510518618517 35.26521543480706 22.9052761840477 20.17099786252672 9 22.098942513218585 21.589042636967037 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34-35 17.76272025494655 30.75953764216631 35.07905902670835 16.398683076178784 36-37 17.759330730368053 30.558915531151193 35.40403159564772 16.27772214283303 38-39 16.753531307835537 31.62187020088093 35.59111083609444 16.033487655189095 40-41 16.06862102619344 31.692575102588407 35.8862541379902 16.352549733227956 42-43 15.74907678504631 32.26386650578961 34.56831790657318 17.41873880259091 44-45 16.801900111936728 31.179723139403414 34.018359873775864 18.000016874883983 46-47 17.919132857181026 32.409575358204066 33.062491826239565 16.608799958375347 48-49 17.46216121579111 31.640437682549372 33.6720530889076 17.225348012751912 50-51 15.983845156500045 32.31024949445802 34.615633411051334 17.0902719379906 52-53 16.49018449769378 33.54047136910789 33.21352233097086 16.755821802227473 54-55 16.03386207672404 32.07334908313646 33.459331758353024 18.43345708178648 56-57 17.315502306221173 32.61643604454944 32.4168916638542 17.651169985375184 58-59 17.95566980444877 31.70120851954247 32.86430662702955 17.478815048979214 60-61 16.89443072578876 32.727009376810535 33.535878815833144 16.842681081567566 62-63 16.990991649768393 32.05290823745145 33.80114130144364 17.154958811336517 64-65 16.89356322517954 33.03352331613504 33.112835159333834 16.960078299351583 66-67 16.72680738563654 32.072675113554865 32.17589402483441 19.02462347597418 68-69 16.145018147659083 31.92342686303545 33.217739565271806 18.713815424033662 70-71 17.63401394982563 32.35318933513331 32.48650016874789 17.52629654629317 72-73 17.57438969512881 30.452806839914505 33.57675216559793 18.39605129935876 74-75 16.23115648554393 33.539627629654625 32.065755428057145 18.16346045674429 76-77 17.094475357375057 31.50951733610283 32.65320195103279 18.74280535548932 78-79 16.835301876948563 31.274319681732976 33.43449102166922 18.455887419649248 80-81 17.30905797763806 31.816334537538726 32.34507867178348 18.529528813039732 82-83 16.672713466081674 31.740212622342224 33.101023737203285 18.48605017437282 84-85 16.16171133101492 31.423854516613957 32.8109641283667 19.603470024004423 86-87 16.44679809056315 31.63813582220621 32.674055977122784 19.241010110107855 88-89 17.166398696805395 30.86476391596428 32.33960980388559 19.629227583344743 90-91 17.091473944401177 29.915123195521392 33.03705459429565 19.95634826578178 92-93 17.27092473844077 31.582433344583194 32.72809089886376 18.41855101811227 94-95 16.91873489172668 30.823338494228093 32.54865233147288 19.70927428257235 96-97 16.20134435819552 30.580914613567327 32.64920125998425 20.568539768252897 98-99 16.869867251659354 30.9366914163573 31.54854314321071 20.64489818877264 100-101 16.890726996082225 29.363340542638493 33.16003813713055 20.585894324148736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14299.0 1 8387.5 2 2037.0 3 1404.0 4 1020.5 5 990.0 6 1691.0 7 3202.5 8 4205.0 9 4018.0 10 2900.5 11 1709.0 12 1402.5 13 1283.5 14 1122.0 15 990.5 16 862.5 17 815.5 18 814.5 19 852.0 20 969.0 21 1081.5 22 1145.5 23 1283.5 24 1411.0 25 1742.5 26 2788.0 27 3732.5 28 3881.0 29 4195.5 30 5251.5 31 6448.5 32 8035.5 33 10402.5 34 12776.0 35 14295.5 36 16411.5 37 18774.0 38 18312.5 39 16418.0 40 14722.0 41 12843.5 42 11201.5 43 10456.5 44 10195.5 45 9875.0 46 9941.0 47 13145.0 48 21490.5 49 21063.0 50 11631.5 51 5735.0 52 2589.5 53 1248.0 54 723.0 55 485.0 56 337.5 57 264.0 58 244.5 59 248.0 60 224.0 61 182.0 62 149.0 63 109.5 64 78.5 65 54.0 66 35.0 67 23.0 68 18.5 69 12.0 70 4.5 71 4.0 72 4.0 73 3.0 74 1.0 75 1.0 76 1.5 77 2.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31837102036224546 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8124648441894476E-4 16-17 0.001546855664304196 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4062324220947238E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009703003712453594 36-37 0.019124760940488244 38-39 0.007312408594892564 40-41 0.003656204297446282 42-43 0.002531218359770503 44-45 5.624929688378895E-4 46-47 4.218697266284171E-4 48-49 0.001546855664304196 50-51 2.8124648441894476E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.8124648441894476E-4 60-61 5.624929688378895E-4 62-63 2.8124648441894476E-4 64-65 4.218697266284171E-4 66-67 0.0014062324220947239 68-69 0.0012656091798852514 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.07340533243334459 78-79 0.0026718416019799753 80-81 1.4062324220947238E-4 82-83 0.0 84-85 1.4062324220947238E-4 86-87 0.07649904376195298 88-89 0.38066711666104175 90-91 0.3273709078636517 92-93 0.0 94-95 4.218697266284171E-4 96-97 0.0 98-99 0.0 100-101 2.8124648441894476E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 355560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.59556755540556 #Duplication Level Percentage of deduplicated Percentage of total 1 71.63073743433705 14.036449544380694 2 9.785572810517554 3.8350770615367304 3 4.114877859746821 2.419001012487344 4 2.3179378247265836 1.816852289346383 5 1.5400292792146282 1.5088873889076386 6 1.1711685851250109 1.3769827877151535 7 0.9960673996038694 1.3662954213072338 8 0.747768177512415 1.172235347058162 9 0.5626202026580934 0.9922375970300372 >10 5.812785257054282 24.303633704578694 >50 0.7936963573212389 10.811958600517494 >100 0.46645807618336826 15.920800989987624 >500 0.03301087923759222 4.531443356958038 >1k 0.022964089904411976 8.935763302958714 >5k 0.002870511238051497 4.064855439307009 >10k+ 0.0014352556190257485 2.907526155923051 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10338 2.907526155923051 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9131 2.5680616492293846 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5322 1.496793790077624 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4542 1.277421532230847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 1.1823602204972437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2959 0.8322083473956576 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2127 0.5982112723590954 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2040 0.5737428282146473 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1988 0.5591180110248622 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1730 0.48655641804477445 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1605 0.45140060749240635 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1600 0.4499943750703116 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1536 0.4319946000674991 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1489 0.4187760152998088 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1456 0.4094948813139836 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1265 0.3557768027899651 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1136 0.31949600629992125 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1058 0.29755878051524354 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1037 0.2916526043424457 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 990 0.27843401957475533 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 954 0.2683091461356733 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 940 0.2643716953538081 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 907 0.2550905613679829 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 882 0.2480593992575093 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 803 0.22584092698841265 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 765 0.21515356058049276 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 752 0.21149735628304644 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 733 0.2061536730790865 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 726 0.2041849476881539 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 724 0.20362245471931603 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 699 0.1965912926088424 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 648 0.1822477219034762 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 618 0.17381032737090785 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 581 0.1634042074474069 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 570 0.1603104961187985 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.15946675666554166 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.1583417707278659 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 547 0.15384182697716278 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 542 0.15243559455506805 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 538 0.1513106086173923 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 536 0.1507481156485544 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 527 0.1482168972887839 TruSeq Adapter, Index 3 (95% over 21bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 465 0.13077961525480933 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 464 0.13049836877039037 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 455 0.12796715041061987 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 453 0.12740465744178198 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 453 0.12740465744178198 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 449 0.1262796715041062 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 435 0.12234222072224096 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 431 0.12121723478456518 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 413 0.1161547980650242 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 410 0.11531105861176734 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 404 0.11362357970525368 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 403 0.11334233322083473 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 382 0.10743615704803688 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 365 0.10265496681291485 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.4062324220947238E-4 0.0 20-21 0.0 0.0 0.0 7.031162110473619E-4 0.0 22-23 0.0 0.0 0.0 0.0021093486331420857 0.0 24-25 0.0 0.0 0.0 0.0030937113286083925 0.0 26-27 0.0 0.0 0.0 0.007734278321520981 0.0 28-29 0.0 0.0 0.0 0.01659354258071774 0.0 30-31 0.0 0.0 0.0 0.045843176960288 0.0 32-33 0.0 0.0 0.0 0.09295196310046125 0.0 34-35 0.0 0.0 0.0 0.16213859826752164 0.0 36-37 0.0 0.0 0.0 0.25719991000112497 0.0 38-39 0.0 0.0 0.0 0.37265159185510177 0.0 40-41 0.0 0.0 0.0 0.49471256609292386 0.0 42-43 0.0 0.0 0.0 0.6254921813477332 0.0 44-45 0.0 0.0 0.0 0.7850995612554843 0.0 46-47 0.0 0.0 0.0 0.9811283608954888 0.0 48-49 0.0 0.0 0.0 1.1640791990100123 0.0 50-51 0.0 0.0 0.0 1.3498425019687255 2.8124648441894476E-4 52-53 0.0 0.0 0.0 1.5334964562942963 2.8124648441894476E-4 54-55 0.0 0.0 0.0 1.746821914726066 2.8124648441894476E-4 56-57 0.0 0.0 0.0 2.021318483518956 2.8124648441894476E-4 58-59 0.0 0.0 0.0 2.2908932388345145 2.8124648441894476E-4 60-61 0.0 0.0 0.0 2.6281077736528293 2.8124648441894476E-4 62-63 0.0 0.0 0.0 2.9502756215547308 2.8124648441894476E-4 64-65 0.0 0.0 0.0 3.281724603442457 2.8124648441894476E-4 66-67 0.0 0.0 0.0 3.668719766002925 2.8124648441894476E-4 68-69 0.0 0.0 0.0 4.111401732478344 2.8124648441894476E-4 70-71 0.0 0.0 0.0 4.603161210484869 2.8124648441894476E-4 72-73 0.0 0.0 0.0 5.100826864664192 2.8124648441894476E-4 74-75 0.0 0.0 0.0 5.6416638542018225 2.8124648441894476E-4 76-77 0.0 0.0 0.0 6.166751040611992 2.8124648441894476E-4 78-79 0.0 0.0 0.0 6.823461581730228 2.8124648441894476E-4 80-81 0.0 0.0 0.0 7.506609292383845 2.8124648441894476E-4 82-83 0.0 0.0 0.0 8.156569917876027 2.8124648441894476E-4 84-85 0.0 0.0 0.0 8.875435932050848 2.8124648441894476E-4 86-87 0.0 0.0 0.0 9.630160872989087 2.8124648441894476E-4 88-89 0.0 0.0 0.0 10.376870289121387 2.8124648441894476E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATAGC 25 0.0050321273 56.960762 8 GTATGGT 40 5.323228E-4 47.607895 1 GTAAGCG 125 0.0 45.56861 94-95 CGCAGAA 215 0.0 41.94785 9 CCTTTAG 80 2.2168388E-7 41.65691 1 CTTTAGG 80 2.2689528E-7 41.53389 2 TCATAAC 60 8.3128514E-5 39.556087 2 GGCATTC 100 3.0391675E-8 37.973843 7 TAGGCAT 90 5.713955E-7 36.919014 5 CTCATAA 70 2.0233306E-4 34.00564 1 ACCCTTG 115 1.0392432E-7 33.020733 7 CATTCCG 115 1.0392432E-7 33.020733 9 CATATAG 160 5.2750693E-11 32.63377 3 GCCTATT 60 0.003960641 31.644869 7 CCTTGGC 120 1.5099431E-7 31.644869 9 TTAGGCA 105 1.9070121E-6 31.644867 4 CCCTTGG 125 2.1598316E-7 30.379074 8 GCATTCC 160 1.9099389E-9 29.667065 8 CATAACC 80 4.5056012E-4 29.667065 3 CATGGGG 305 0.0 28.01349 4 >>END_MODULE