##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938890_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 354539 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37868612479868 33.0 31.0 34.0 31.0 34.0 2 32.49502593508753 34.0 31.0 34.0 31.0 34.0 3 32.48941301239074 34.0 31.0 34.0 31.0 34.0 4 35.983028665393654 37.0 37.0 37.0 35.0 37.0 5 36.013369474162225 37.0 37.0 37.0 35.0 37.0 6 36.13081776616959 37.0 37.0 37.0 35.0 37.0 7 36.11226973619263 37.0 37.0 37.0 35.0 37.0 8 36.11352770781212 37.0 37.0 37.0 35.0 37.0 9 37.8307746115378 39.0 38.0 39.0 37.0 39.0 10-11 37.766949475234036 39.0 38.0 39.0 35.0 39.0 12-13 37.7005082656633 39.0 38.0 39.0 35.0 39.0 14-15 39.00816976411622 40.5 39.0 41.0 36.0 41.0 16-17 38.89600720936201 40.0 38.5 41.0 36.0 41.0 18-19 38.77600348621731 40.0 38.0 41.0 35.0 41.0 20-21 38.67816234603245 40.0 38.0 41.0 35.0 41.0 22-23 38.71552494930036 40.0 38.0 41.0 35.0 41.0 24-25 38.64709242142614 40.0 38.0 41.0 35.0 41.0 26-27 38.520457551919534 40.0 38.0 41.0 35.0 41.0 28-29 38.38859617700732 40.0 38.0 41.0 35.0 41.0 30-31 38.19305492484607 40.0 37.5 41.0 35.0 41.0 32-33 37.943024603781254 40.0 36.5 41.0 35.0 41.0 34-35 37.65463602029678 40.0 36.0 41.0 34.5 41.0 36-37 37.38187759315618 40.0 35.0 41.0 34.0 41.0 38-39 37.18632787930242 40.0 35.0 41.0 33.5 41.0 40-41 37.09600777347485 40.0 35.0 41.0 33.0 41.0 42-43 36.883908399358035 40.0 35.0 41.0 33.0 41.0 44-45 36.688416507069746 40.0 35.0 41.0 33.0 41.0 46-47 36.368179523268246 40.0 35.0 41.0 31.5 41.0 48-49 36.130398912390454 40.0 35.0 41.0 31.0 41.0 50-51 35.00391212250274 38.5 34.0 40.0 28.0 40.5 52-53 35.228091972956435 38.5 35.0 40.0 29.0 40.5 54-55 35.762195414326776 39.5 35.0 41.0 30.5 41.0 56-57 35.70512270864418 39.5 35.0 41.0 29.5 41.0 58-59 35.58262983762012 39.0 35.0 41.0 29.0 41.0 60-61 35.339038864553686 39.0 35.0 41.0 28.0 41.0 62-63 35.04439285945975 39.0 35.0 40.5 27.5 41.0 64-65 34.67129850312659 38.0 35.0 40.0 26.0 41.0 66-67 34.270992753970646 37.5 35.0 40.0 26.0 41.0 68-69 33.874810669630136 37.0 35.0 39.0 25.5 41.0 70-71 33.39771365068441 36.0 34.0 39.0 23.5 40.5 72-73 32.9255667218557 36.0 34.0 38.5 21.5 40.0 74-75 32.450272043414124 35.0 33.5 37.0 19.5 40.0 76-77 32.02027844609479 35.0 33.0 37.0 16.0 39.0 78-79 31.556793469829834 35.0 33.0 36.5 9.0 39.0 80-81 31.10274046014684 35.0 32.5 36.0 2.0 38.0 82-83 30.71628368106189 35.0 32.0 35.5 2.0 37.0 84-85 30.347458248598883 35.0 32.0 35.0 2.0 37.0 86-87 30.027554091369357 35.0 31.5 35.0 2.0 36.0 88-89 29.576308953316843 34.5 31.0 35.0 2.0 36.0 90-91 29.16138139950753 34.0 31.0 35.0 2.0 36.0 92-93 28.79310597705753 34.0 30.0 35.0 2.0 35.0 94-95 28.339940034805764 34.0 29.5 35.0 2.0 35.0 96-97 27.804815549206154 34.0 29.0 35.0 2.0 35.0 98-99 27.276202335991243 34.0 28.0 35.0 2.0 35.0 100-101 24.773302796025263 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029238489253074817 1101 2 0.2586365253155023 1101 3 0.1679889719456753 1101 4 0.31469672665703996 1101 5 0.08650761505072779 1101 6 0.07793648272787124 1101 7 0.10581015534669547 1101 8 0.035989136776528596 1101 9 0.10865112503397256 1101 10-11 0.32662420035789097 1101 12-13 0.2698491764914053 1101 14-15 -0.034571899092092906 1101 16-17 0.20862305307623075 1101 18-19 0.45301065679546326 1101 20-21 0.37492384663189426 1101 22-23 0.500059028403534 1101 24-25 0.2443175119992418 1101 26-27 0.3546853189566477 1101 28-29 0.4804837268013742 1101 30-31 0.5669611505588747 1101 32-33 0.47521053188601314 1101 34-35 0.4587134684111689 1101 36-37 0.5056299679149276 1101 38-39 0.5569047427400662 1101 40-41 0.5448784575934695 1101 42-43 0.8871462658020022 1101 44-45 0.9313503683627005 1101 46-47 0.8188490151907857 1101 48-49 0.7711131888202232 1101 50-51 0.3865113739023869 1101 52-53 0.25962944907539764 1101 54-55 0.29195749550454764 1101 56-57 0.48165878799996165 1101 58-59 0.45048674665150656 1101 60-61 0.1606164234179559 1101 62-63 0.39635655313029616 1101 64-65 0.4713647486399992 1101 66-67 0.4931104816845391 1101 68-69 0.4348196777062583 1101 70-71 0.4308634034509211 1101 72-73 0.3830310764440483 1101 74-75 0.6113729039831526 1101 76-77 0.6540815772358677 1101 78-79 0.4897212658168364 1101 80-81 0.6318693294439583 1101 82-83 0.39112204207266643 1101 84-85 0.15255495351453163 1101 86-87 0.21652160332093118 1101 88-89 0.031400006792505764 1101 90-91 0.3171853969044989 1101 92-93 0.5387552453393205 1101 94-95 0.6377684852877046 1101 96-97 0.9663577181640264 1101 98-99 0.8911023303432124 1101 100-101 1.4162152222649915 1102 1 0.13605990857449513 1102 2 0.17128868796766028 1102 3 -0.061129132172425216 1102 4 0.07241007812039157 1102 5 0.08904905509216832 1102 6 0.15122546226685074 1102 7 0.3893021263386629 1102 8 -0.03542209088470116 1102 9 0.2281959320787763 1102 10-11 0.10338216461585858 1102 12-13 0.3197026794699056 1102 14-15 0.25530200015680293 1102 16-17 0.1287781294813115 1102 18-19 0.16688012066492774 1102 20-21 0.005468070926120561 1102 22-23 0.2424185207630316 1102 24-25 0.16513627656800622 1102 26-27 0.10936351113483767 1102 28-29 0.1726133564310075 1102 30-31 0.21823348933121878 1102 32-33 0.22688593981141736 1102 34-35 0.08054880899650385 1102 36-37 0.1395411955761574 1102 38-39 0.23675614134146628 1102 40-41 0.35995631954632756 1102 42-43 0.23308158048732253 1102 44-45 0.29046636810370074 1102 46-47 0.2828358385526073 1102 48-49 0.3907551209621545 1102 50-51 0.33431877483478445 1102 52-53 0.4216867205076653 1102 54-55 0.7294291673512276 1102 56-57 0.6502841441253153 1102 58-59 0.6365581983479558 1102 60-61 0.5541511294526629 1102 62-63 0.5499315660803106 1102 64-65 0.4568202653455202 1102 66-67 0.5719436855177378 1102 68-69 0.5817132933498712 1102 70-71 0.388788002000517 1102 72-73 0.4603750287879933 1102 74-75 0.6339180671533171 1102 76-77 0.3496793822086737 1102 78-79 0.33055751290308777 1102 80-81 0.2235918430414685 1102 82-83 0.39091160436222694 1102 84-85 0.345777240486818 1102 86-87 0.5454316978560243 1102 88-89 0.40332033183783267 1102 90-91 0.18093102627513247 1102 92-93 0.23529597313005013 1102 94-95 0.1436000379942577 1102 96-97 -0.027215837909530194 1102 98-99 0.10807170356258666 1102 100-101 0.16978052270529176 1103 1 0.13472591647963128 1103 2 0.2716180687318257 1103 3 0.445952554257083 1103 4 0.3515355721915441 1103 5 0.352109002391245 1103 6 0.4292804688544649 1103 7 0.4196216256799019 1103 8 0.2847442464012033 1103 9 0.5391478424872105 1103 10-11 0.5410797930743527 1103 12-13 0.5661083118809742 1103 14-15 0.7401710713030525 1103 16-17 0.6336447302718184 1103 18-19 0.7191199098612415 1103 20-21 0.8583748561698599 1103 22-23 0.6540785998144116 1103 24-25 0.7377400315087144 1103 26-27 0.6347422990136238 1103 28-29 0.4753307477353488 1103 30-31 0.5380128042192283 1103 32-33 0.6113309332238046 1103 34-35 0.6273620501032227 1103 36-37 0.6467299307540202 1103 38-39 0.8051767605773037 1103 40-41 0.833445285290729 1103 42-43 0.6462321074965445 1103 44-45 0.944530103281565 1103 46-47 1.0639499689873944 1103 48-49 0.8908704042296165 1103 50-51 0.7132672267451952 1103 52-53 0.7588614570030572 1103 54-55 0.7457541014750717 1103 56-57 0.6334363180383562 1103 58-59 0.6157536858314927 1103 60-61 0.6630938171997016 1103 62-63 0.6591089376218164 1103 64-65 0.700981991300722 1103 66-67 0.9945721440157627 1103 68-69 1.0225318045488194 1103 70-71 1.1029307885617854 1103 72-73 1.1327399826746856 1103 74-75 1.0371254123443592 1103 76-77 1.2146538551994546 1103 78-79 1.1698773416250923 1103 80-81 1.2602231012759901 1103 82-83 1.212700141911636 1103 84-85 1.3160753300783838 1103 86-87 1.1595415463408756 1103 88-89 1.089383408254168 1103 90-91 1.1585454860906772 1103 92-93 1.0138450508639103 1103 94-95 1.5209098206029523 1103 96-97 1.0762920672288132 1103 98-99 0.6947564861712827 1103 100-101 1.134969093192776 1104 1 0.15744361745819901 1104 2 0.10194136862034497 1104 3 -0.09696829301158516 1104 4 0.20928362124394084 1104 5 0.09505462359773276 1104 6 0.1890475938389855 1104 7 0.12575317528971652 1104 8 0.09581819660559177 1104 9 0.2938363102191559 1104 10-11 0.14428801802170454 1104 12-13 0.13452154116377102 1104 14-15 0.22468736016716662 1104 16-17 0.09038943484261353 1104 18-19 -0.009952306167498648 1104 20-21 0.0330435047515536 1104 22-23 0.031398059742564044 1104 24-25 0.04631181399354034 1104 26-27 -0.03277906850774315 1104 28-29 -0.030912284594627693 1104 30-31 0.17807226166998902 1104 32-33 0.3569769136523959 1104 34-35 0.37751688721458265 1104 36-37 0.5740060362909958 1104 38-39 0.5982152840506032 1104 40-41 0.84117475388976 1104 42-43 0.8857217643775073 1104 44-45 1.1094130464253809 1104 46-47 1.0823817787235512 1104 48-49 1.2597171774242213 1104 50-51 1.2298162172072225 1104 52-53 1.1912533806993295 1104 54-55 1.3788520823991348 1104 56-57 1.4617157680569406 1104 58-59 1.222566518731277 1104 60-61 1.4540637168652566 1104 62-63 1.4177240744978192 1104 64-65 1.301849079124331 1104 66-67 1.478865467439519 1104 68-69 1.1784094212960028 1104 70-71 1.0106354832230053 1104 72-73 1.0568202502332156 1104 74-75 1.1213444139546667 1104 76-77 0.9047938279481222 1104 78-79 1.0851628612584356 1104 80-81 0.9310146285892529 1104 82-83 0.89112204207267 1104 84-85 0.7978540988136764 1104 86-87 0.9729318597311867 1104 88-89 1.2707162461087442 1104 90-91 0.9938235735426773 1104 92-93 1.2680999746840484 1104 94-95 1.1833525308717476 1104 96-97 1.261229513035822 1104 98-99 1.453780393021276 1104 100-101 1.3378676439174129 1105 1 0.21055301750637767 1105 2 0.26729981969307914 1105 3 0.31725014161501264 1105 4 0.3563270540016532 1105 5 0.1997534017659035 1105 6 0.3275523241804521 1105 7 0.1632055188645083 1105 8 0.28699210206520576 1105 9 0.3691812222987636 1105 10-11 0.33434890196014777 1105 12-13 0.14189738527431217 1105 14-15 0.43106979103939125 1105 16-17 0.34081927710918336 1105 18-19 0.21611150152896386 1105 20-21 0.2084149964495765 1105 22-23 0.43159159258915736 1105 24-25 0.42791622621020053 1105 26-27 0.545140744105943 1105 28-29 0.389996738355201 1105 30-31 0.38916842174574384 1105 32-33 0.3083943548657615 1105 34-35 0.435132363197404 1105 36-37 0.5330526586029265 1105 38-39 0.3514775169046729 1105 40-41 0.1024316375956289 1105 42-43 0.3356773265779651 1105 44-45 0.20898694191763667 1105 46-47 0.11451404554969002 1105 48-49 0.10664764680366545 1105 50-51 0.10363778490634701 1105 52-53 0.09096100489674797 1105 54-55 -0.008101478023810671 1105 56-57 -0.013527809227454668 1105 58-59 0.05113628607655585 1105 60-61 0.1775443178968743 1105 62-63 -0.06710477767797585 1105 64-65 -0.00689395410849869 1105 66-67 -0.11037144832801005 1105 68-69 0.06557063498782867 1105 70-71 0.0547244252711252 1105 72-73 -0.31755367516111477 1105 74-75 -0.4804264531223268 1105 76-77 -0.5829546291880909 1105 78-79 -0.6525378356259353 1105 80-81 -0.6080449512866508 1105 82-83 -0.46429313987312426 1105 84-85 -0.2326169418614441 1105 86-87 -0.3832184147864339 1105 88-89 -0.2654735655912681 1105 90-91 -0.09041720457565106 1105 92-93 0.2604500282585924 1105 94-95 0.16277824499134042 1105 96-97 -0.30506171652071146 1105 98-99 -0.9047745063499519 1105 100-101 -0.2836095183760783 1106 1 -0.002100542085955226 1106 2 0.07630034297931587 1106 3 0.1423479463046533 1106 4 0.03549444745476649 1106 5 0.00958453812764759 1106 6 0.04089944569083315 1106 7 -0.07082802129148291 1106 8 0.06447916526655462 1106 9 0.0887081050909515 1106 10-11 0.06166693540063051 1106 12-13 0.08893749557972086 1106 14-15 0.3115819470617538 1106 16-17 0.3211586656118399 1106 18-19 0.24645795024276396 1106 20-21 0.20540817711622594 1106 22-23 0.10832113666564425 1106 24-25 0.29132605900778685 1106 26-27 0.36465682892815465 1106 28-29 0.338033584351237 1106 30-31 0.28490986850759725 1106 32-33 0.23446979114527267 1106 34-35 0.14389865359635223 1106 36-37 -0.27784581556085186 1106 38-39 -0.07414938831406204 1106 40-41 -0.033469120754105575 1106 42-43 0.25609213474787396 1106 44-45 0.052432989445314604 1106 46-47 -0.08428488794312017 1106 48-49 -0.25737683966980285 1106 50-51 -0.12915814176712814 1106 52-53 0.17273486218081047 1106 54-55 -0.07556387201681503 1106 56-57 -0.048255741914566386 1106 58-59 0.16416196032672303 1106 60-61 0.42414918695072146 1106 62-63 0.26672692350066995 1106 64-65 0.16367244094768552 1106 66-67 0.16547515404920432 1106 68-69 0.08439232727890555 1106 70-71 -0.016430043842525777 1106 72-73 0.15511084852381174 1106 74-75 -0.3644105718003203 1106 76-77 -0.19919477604048197 1106 78-79 -0.006005229909657572 1106 80-81 -0.034797337222713054 1106 82-83 0.012204663155291229 1106 84-85 0.04714184810142541 1106 86-87 0.007119893919220743 1106 88-89 0.11259658798908845 1106 90-91 0.18898026869937468 1106 92-93 0.3407495473336226 1106 94-95 0.37708472460394304 1106 96-97 0.5375827893890985 1106 98-99 0.40392284316372695 1106 100-101 0.6341639402137069 1107 1 0.09393494170463157 1107 2 0.057387283249930476 1107 3 0.26477200750422014 1107 4 0.21930081493459852 1107 5 0.11812074870467626 1107 6 0.26049693059444223 1107 7 0.2011063935000763 1107 8 0.2636684869048622 1107 9 0.1155369074299557 1107 10-11 0.1637949661000846 1107 12-13 0.27163499460375107 1107 14-15 0.11911643826971385 1107 16-17 0.23877846078266174 1107 18-19 0.32835639948815043 1107 20-21 0.29733627924841244 1107 22-23 0.1429618549798306 1107 24-25 0.3010940687757966 1107 26-27 0.3352282076628015 1107 28-29 0.39874309404033426 1107 30-31 0.16905180366993733 1107 32-33 0.2894439175479704 1107 34-35 0.01822274220615583 1107 36-37 0.12488939329679738 1107 38-39 0.34856206500963083 1107 40-41 3.908212690930668E-4 1107 42-43 0.0033158105429791362 1107 44-45 0.2585496241333871 1107 46-47 0.3564965528383155 1107 48-49 0.5323036234800469 1107 50-51 0.3645707295127565 1107 52-53 0.3664712089783748 1107 54-55 0.3956886094397447 1107 56-57 0.42087452313406715 1107 58-59 0.1648389099832599 1107 60-61 -0.009044603385923722 1107 62-63 0.11511096576226265 1107 64-65 0.23071537533756725 1107 66-67 0.012077531263827268 1107 68-69 0.1020137836758721 1107 70-71 0.2573483319766652 1107 72-73 0.15037635399340132 1107 74-75 0.24552106874356383 1107 76-77 0.47535440871278567 1107 78-79 0.3241385492137141 1107 80-81 0.5625089845734053 1107 82-83 0.4540742070656485 1107 84-85 0.254263524610856 1107 86-87 0.32815184760423577 1107 88-89 0.33365179884021856 1107 90-91 0.2769297341998609 1107 92-93 -0.24392314958397066 1107 94-95 0.145285533621081 1107 96-97 -0.27882264946531876 1107 98-99 -0.32459957923624927 1107 100-101 -0.015387944032053014 1108 1 -0.16018238158372355 1108 2 -0.21641579044477766 1108 3 -0.36948928234673417 1108 4 -0.5188761246149198 1108 5 -0.49678159655707077 1108 6 -0.46652561040678364 1108 7 -0.3823021911727338 1108 8 -0.263004887881209 1108 9 -0.2216062937190273 1108 10-11 -0.22064907700387693 1108 12-13 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-1.997777722168209 2316 92-93 -2.4432921339683347 2316 94-95 -2.594456468855057 2316 96-97 -2.9592052526044235 2316 98-99 -3.953486515615495 2316 100-101 -4.463045598091721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2295.0 3 368.0 4 280.0 5 249.0 6 308.0 7 317.0 8 368.0 9 325.0 10 404.0 11 567.0 12 892.0 13 1648.0 14 1724.0 15 1816.0 16 2745.0 17 4230.0 18 3331.0 19 1683.0 20 1475.0 21 1338.0 22 1422.0 23 1606.0 24 1742.0 25 2088.0 26 2332.0 27 2759.0 28 3251.0 29 4048.0 30 5013.0 31 6339.0 32 8484.0 33 11998.0 34 19328.0 35 41322.0 36 43177.0 37 74276.0 38 84605.0 39 14384.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.52318082998086 24.708879081983156 18.01373406574145 17.754206022294532 2 13.65421038221289 25.782222485668076 39.720107598813364 20.843459533305673 3 18.108682581486534 30.69572998439004 34.49208352034235 16.70350391378108 4 10.244321101280047 21.52504474797152 42.62152532706956 25.609108823678877 5 9.238231291748775 41.165775461459845 37.57178107407638 12.024212172715004 6 22.127795292703098 42.03826689735563 20.04256267975535 15.791375130185914 7 20.05317932780652 38.52001140363505 25.859153816407122 15.567655452151307 8 21.191490322598856 34.35597368175929 23.545567194216986 20.906968801424867 9 22.098553899221766 21.231966081696367 27.257715313199515 29.411764705882355 10-11 20.57546727784897 29.23287965090351 30.28400783547755 19.907645235769973 12-13 23.786646192409094 29.52992948801183 29.740787995461037 16.942636324118034 14-15 21.05901283010346 30.420895143191856 31.27962285988511 17.240469166819576 16-17 15.504262165518798 33.637377215761546 32.468104324263294 18.39025629445636 18-19 15.248234958686318 33.34029657775356 34.91351932723767 16.497949136322447 20-21 16.76064707451576 31.397595701225576 33.783968800857046 18.057788423401615 22-23 16.467789863000164 30.8124704437496 34.39366455155669 18.326075141693547 24-25 17.69008572493477 32.07640135985272 33.65649600740917 16.57701690780334 26-27 17.156313929569293 30.536680130848698 36.13558680726642 16.17141913231559 28-29 15.504092655807506 31.997114388247482 35.885207992749265 16.613584963195745 30-31 15.988859197020838 31.87099706798685 34.93542371613257 17.20472001885975 32-33 16.30191747408361 31.951549337127627 35.00942927479278 16.73710391399598 34-35 17.458680170868334 30.978304701992098 34.30469067256414 17.258324454575423 36-37 17.5179833171513 30.530605302509517 35.39031771117552 16.56109366916366 38-39 16.79912362996324 31.687069007054607 35.02107312814786 16.49273423483429 40-41 15.73789164102477 32.089913809224285 35.79892219029971 16.373272359451235 42-43 15.565351947422567 32.42147535361951 33.96093535231585 18.052237346642066 44-45 16.18845139892848 31.665957989624964 33.73005074127619 18.415539870170367 46-47 17.532616613427585 32.408570027578506 33.02731490798189 17.031498451012013 48-49 16.829345043530523 31.849147107934716 33.49780717296827 17.823700675566492 50-51 15.68792255467521 32.23945119272037 34.86882202032515 17.20380423227928 52-53 16.037618176921658 33.42915054357841 33.2935039900214 17.23972728947853 54-55 15.749752328958166 31.887274598092613 33.88891014820579 18.474062924743436 56-57 17.12962766176006 32.5288367671083 32.33005045460148 18.011485116530153 58-59 17.56034735593782 31.45699352072645 33.267874676425954 17.71478444690977 60-61 16.909099921399793 31.982070655214166 34.06164805517593 17.04718136821011 62-63 16.791727425647306 31.830195123126394 33.98605838247194 17.392019068754365 64-65 16.674935995395636 32.604356565894264 33.46172056851399 17.25898687019611 66-67 16.461045957717086 31.75174099446511 32.44757168133563 19.339641366482173 68-69 15.699095471687412 31.75206283494485 33.88493492500071 18.663906768367028 70-71 17.407756061460987 32.00091301154874 32.51951491299454 18.07181601399573 72-73 17.00037708994304 30.461495714500387 34.0200339664475 18.518093229109077 74-75 16.059437183963528 33.06384734135979 32.647356102844135 18.229359371832548 76-77 16.846391749654867 31.27131400579996 33.076927438123825 18.805366806421343 78-79 16.70372244021125 31.010510236037486 33.757455437421086 18.528311886330176 80-81 17.253672803119617 31.592908315951757 32.50274893443366 18.650669946494965 82-83 16.553172613471613 31.36815014377488 33.65862783918188 18.420049403571625 84-85 16.567596986240794 30.941771405570577 32.87582699797609 19.614804610212538 86-87 16.654667898666155 31.460911415079902 33.152454267687794 18.731966418566156 88-89 17.622246191832158 30.425797599794763 32.569030350534284 19.382925857838796 90-91 16.91641658598405 29.83177850955221 33.60871740859238 19.643087495871363 92-93 17.751365206099337 31.115783086755087 33.17918649027202 17.953665216873553 94-95 17.34309234915275 30.138120411284962 33.03506242566264 19.48372481389965 96-97 16.548627905723983 29.837202906645423 33.40856059811345 20.205608589517137 98-99 17.357354292130246 30.26266544180191 32.07798872670772 20.30199153936012 100-101 17.550369577696358 29.083886695911616 33.25844926190763 20.107294464484397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15379.0 1 8630.5 2 1710.0 3 1351.5 4 995.0 5 1053.5 6 1846.5 7 3316.0 8 4458.5 9 4201.0 10 2806.5 11 1674.5 12 1432.0 13 1372.0 14 1242.0 15 1088.5 16 993.5 17 932.5 18 894.5 19 917.5 20 1024.0 21 1096.5 22 1168.5 23 1328.5 24 1453.5 25 1744.5 26 2613.5 27 3564.5 28 3704.0 29 3909.0 30 5046.0 31 6155.5 32 7714.0 33 10254.5 34 12811.5 35 14148.0 36 16095.5 37 18683.0 38 18209.0 39 16093.0 40 14058.5 41 12332.0 42 11134.0 43 10317.0 44 9940.5 45 9572.0 46 9690.5 47 12576.5 48 23531.0 49 22501.5 50 9738.0 51 4545.0 52 2204.0 53 1410.5 54 952.5 55 677.5 56 521.5 57 444.5 58 429.0 59 402.0 60 351.0 61 308.0 62 266.0 63 227.0 64 184.5 65 148.5 66 116.5 67 90.5 68 71.0 69 56.5 70 51.5 71 49.5 72 44.0 73 36.5 74 34.0 75 28.5 76 19.5 77 15.5 78 11.0 79 7.5 80 7.5 81 4.0 82 4.0 83 3.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23128626187810084 2 0.07333466840037345 3 0.07841168390501468 4 0.0939247868358629 5 0.09505301250356096 6 0.06712942722803415 7 0.07502700690192052 8 0.07333466840037345 9 0.07784757107116566 10-11 0.07136027348190185 12-13 0.07700140182039211 14-15 0.0829245865758069 16-17 0.07305261198344894 18-19 0.08405281224350496 20-21 0.0841938404519672 22-23 0.09632226637972127 24-25 0.10802760768208856 26-27 0.10986097439209791 28-29 0.10379676142822088 30-31 0.09745049204741932 32-33 0.09110422266661777 34-35 0.3293008667593692 36-37 0.5024835067510203 38-39 0.48513703711016276 40-41 0.4515723234961457 42-43 0.47611123176857834 44-45 0.49895780153946395 46-47 0.48739348844555885 48-49 0.5071374376302747 50-51 0.5003680836240865 52-53 0.5031886477933316 54-55 0.4943038706602095 56-57 0.4646879468831355 58-59 0.46384177763236206 60-61 0.419276863758289 62-63 0.4824575011493799 64-65 0.5161632429718592 66-67 0.5265993303980662 68-69 0.5271634432319152 70-71 0.5246249354795947 72-73 0.5184196943072553 74-75 0.5163042711803215 76-77 0.5014963092917846 78-79 0.4773804856447387 80-81 0.4724444983485597 82-83 0.4880986294878702 84-85 0.49557312453636976 86-87 0.4865473191947854 88-89 0.5013552810833223 90-91 0.5132016505941518 92-93 0.5203940892257269 94-95 0.5188427789326421 96-97 0.5161632429718592 98-99 0.520958202059576 100-101 0.49966294258177524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 354539.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.57584356022892 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61258717076826 14.018768033982157 2 9.766007722897815 3.823556787828702 3 4.200046106852631 2.4665833660048686 4 2.270762492075385 1.7780836522921315 5 1.5229669759668032 1.4906681634460526 6 1.2564117341939947 1.4757191733490533 7 0.8789118782779091 1.2043809002676715 8 0.7622039075557605 1.1936627564245401 9 0.6109157973603827 1.0763272869839424 >10 5.763356578871535 23.940948668552682 >50 0.8183966341997578 11.065637348782504 >100 0.4797994351910553 16.131934709580612 >500 0.028816782894357674 4.071766434722273 >1k 0.025935104604921908 10.854095036089118 >5k 0.0014408391447178837 2.504378925872753 >10k+ 0.0014408391447178837 2.9034887558209395 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10294 2.9034887558209395 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8879 2.504378925872753 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4732 1.3346909648867966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4202 1.1852010639168047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3986 1.1242768778611099 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3552 1.001864392915871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2729 0.7697319617869967 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2255 0.6360372201647774 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2235 0.6303960918262871 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1947 0.5491638437520273 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1724 0.4862652627778608 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1541 0.43464893848067493 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1332 0.3756991473434516 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1304 0.3678015676695653 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1234 0.34805761848484934 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1229 0.34664733640022677 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1194 0.3367753618078688 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1124 0.3170314126231529 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.3051850431123233 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1080 0.30462093027847426 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 988 0.2786717399214191 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 924 0.26062012923825023 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 904 0.25497900089975994 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 846 0.2386197287181382 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 840 0.23692739021659112 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 823 0.23213243112887438 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.23100420546117634 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 803 0.22649130279038415 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 765 0.21577315894725263 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 719 0.20279856376872502 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 707 0.19941388676563088 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 688 0.19405481484406512 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 655 0.18474695308555616 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 605 0.1706441322393305 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 590 0.16641328598546282 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 583 0.16443889106699122 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.1573874806438784 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 552 0.15569514214233132 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 547 0.15428486005770875 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.1466693368007469 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 476 0.1342588544560683 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 457 0.12889978253450254 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 442 0.12466893628063486 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 440 0.12410482344678583 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 438 0.12354071061293681 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 421 0.11874575152522007 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 412 0.11620724377289947 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 389 0.10971994618363566 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 377 0.10633526918054148 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 376 0.10605321276361697 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 371 0.10464293067899441 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 365 0.10295059217744734 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 365 0.10295059217744734 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 365 0.10295059217744734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0014102820846225662 0.0 22-23 0.0 0.0 0.0 0.002961592377707389 0.0 24-25 0.0 0.0 0.0 0.005641128338490265 0.0 26-27 0.0 0.0 0.0 0.00958991817543345 1.4102820846225662E-4 28-29 0.0 0.0 0.0 0.024397880063970395 2.8205641692451324E-4 30-31 0.0 0.0 0.0 0.05641128338490265 2.8205641692451324E-4 32-33 0.0 0.0 0.0 0.1063352691805415 2.8205641692451324E-4 34-35 0.0 0.0 0.0 0.17416983745088693 2.8205641692451324E-4 36-37 0.0 0.0 0.0 0.2781076270875701 2.8205641692451324E-4 38-39 0.0 0.0 0.0 0.4147639610874967 2.8205641692451324E-4 40-41 0.0 0.0 0.0 0.5497279565858764 2.8205641692451324E-4 42-43 0.0 0.0 0.0 0.6892048547550481 2.8205641692451324E-4 44-45 0.0 0.0 0.0 0.8582976767012938 2.8205641692451324E-4 46-47 0.0 0.0 0.0 1.053480717213057 2.8205641692451324E-4 48-49 0.0 0.0 0.0 1.2479586166825087 2.8205641692451324E-4 50-51 0.0 0.0 0.0 1.4542828856627903 2.8205641692451324E-4 52-53 0.0 0.0 0.0 1.6607481828515338 2.8205641692451324E-4 54-55 0.0 0.0 0.0 1.8879446266842295 2.8205641692451324E-4 56-57 0.0 0.0 0.0 2.1673215076479595 2.8205641692451324E-4 58-59 0.0 0.0 0.0 2.4495189527809353 2.8205641692451324E-4 60-61 0.0 0.0 0.0 2.804910038105822 2.8205641692451324E-4 62-63 0.0 0.0 0.0 3.13787763828521 2.8205641692451324E-4 64-65 0.0 0.0 0.0 3.480576184848493 2.8205641692451324E-4 66-67 0.0 0.0 0.0 3.877711619878208 2.8205641692451324E-4 68-69 0.0 0.0 0.0 4.351707428519852 2.8205641692451324E-4 70-71 0.0 0.0 0.0 4.885640225757956 2.8205641692451324E-4 72-73 0.0 0.0 0.0 5.360482203650374 2.8205641692451324E-4 74-75 0.0 0.0 0.0 5.937428604469465 2.8205641692451324E-4 76-77 0.0 0.0 0.0 6.492092548351521 2.8205641692451324E-4 78-79 0.0 0.0 0.0 7.184258995484276 2.8205641692451324E-4 80-81 0.0 0.0 0.0 7.930580274666539 2.8205641692451324E-4 82-83 0.0 0.0 0.0 8.596656503233778 2.8205641692451324E-4 84-85 0.0 0.0 0.0 9.352567700591472 2.8205641692451324E-4 86-87 0.0 0.0 0.0 10.167287660877928 2.8205641692451324E-4 88-89 0.0 0.0 0.0 10.989059031587498 2.8205641692451324E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGTA 25 0.0050757118 56.836742 2 GTCTGTA 25 0.0050785495 56.828716 7 CGCAGAA 175 0.0 43.29807 9 ATATGAG 175 0.0 35.184647 3 TAGGCAT 125 5.7880243E-9 34.102047 5 GGATATG 185 0.0 33.325127 1 CCTTTAG 130 8.381903E-9 32.83215 1 CATTCCG 145 6.82121E-10 32.660183 9 GGCATTC 145 6.82121E-10 32.660183 7 GATATGA 190 0.0 32.406914 2 GGACAAT 205 0.0 32.34155 6 TTAGGCA 135 1.2259989E-8 31.575966 4 GGCTCGT 30 0.004008555 31.571512 10-11 GACAATG 210 0.0 31.571508 7 CTTTAGG 140 1.7469574E-8 30.448257 2 AGGACAA 220 0.0 30.140696 5 GATTGGC 205 1.8189894E-12 30.031437 9 GCTTTGC 210 1.8189894E-12 29.35785 1 CATATAG 100 5.0411698E-5 28.418371 3 TGAGATG 105 7.02847E-5 27.065115 4 >>END_MODULE