FastQCFastQC Report
Thu 26 May 2016
SRR938889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144866
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127150.40430975437427225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.31314529776467426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.21097878256180072No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57280.18213812607595997No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33310.10591866235318134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200650.023.4668751
GTACATG104000.023.2766061
TACATGG102950.022.4099672
GGTATCA149750.021.839121
GAGTACT100550.021.54503612-13
ACATGGG108600.020.4152493
ATCAACG228950.020.381413
TCAACGC230200.020.3118954
CAACGCA229550.020.286865
TATCAAC233700.020.0076942
AACGCAG234700.019.8123786
GTACTTT108650.019.63437714-15
AGTACTT106700.019.39345712-13
AGAGTAC172750.018.48851810-11
CATGGGG55200.018.108484
ACGCAGA255450.017.9805957
CGCAGAG257250.017.6154928
GCAGAGT262650.016.658389
TACTTTT125100.016.4279914-15
ACTTTTT132650.016.42333416-17
Produced by FastQC (version 0.11.5)