##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938889_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3144866 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.979208335108712 33.0 31.0 34.0 30.0 34.0 2 32.089506198356304 33.0 31.0 34.0 30.0 34.0 3 32.137057985936444 34.0 31.0 34.0 30.0 34.0 4 35.66843420355589 37.0 35.0 37.0 35.0 37.0 5 35.698410997479705 37.0 35.0 37.0 35.0 37.0 6 35.77877467593214 37.0 35.0 37.0 35.0 37.0 7 35.70880062934319 37.0 35.0 37.0 33.0 37.0 8 35.697510164185054 37.0 35.0 37.0 33.0 37.0 9 37.34273256793771 39.0 37.0 39.0 34.0 39.0 10-11 37.22976511558839 39.0 37.0 39.0 34.0 39.0 12-13 37.12246006666103 39.0 37.0 39.0 33.5 39.0 14-15 38.364812682002984 40.0 38.0 41.0 34.0 41.0 16-17 38.27159996642146 40.0 38.0 41.0 33.0 41.0 18-19 38.17643772421464 40.0 38.0 41.0 33.5 41.0 20-21 38.303222935412826 40.0 38.0 41.0 34.0 41.0 22-23 38.248374175561054 40.0 38.0 41.0 34.0 41.0 24-25 38.17071108912113 40.0 38.0 41.0 33.5 41.0 26-27 38.05087911535817 40.0 38.0 41.0 33.0 41.0 28-29 37.92244979595315 40.0 38.0 41.0 33.0 41.0 30-31 37.735746292528844 40.0 37.5 41.0 33.0 41.0 32-33 37.53445138838984 40.0 37.0 41.0 32.5 41.0 34-35 37.22867381312908 40.0 37.0 41.0 31.5 41.0 36-37 36.97317500968245 40.0 36.5 41.0 31.0 41.0 38-39 36.747541548670114 39.5 36.0 41.0 30.5 41.0 40-41 36.60155377049451 39.0 36.0 41.0 30.0 41.0 42-43 36.39977474397955 39.0 35.5 41.0 30.0 41.0 44-45 36.34731400320395 39.0 36.0 41.0 30.0 41.0 46-47 36.144373718943825 39.0 36.0 41.0 29.5 41.0 48-49 35.9174940999076 39.0 35.0 41.0 28.0 41.0 50-51 34.83883653548355 38.0 34.0 40.0 25.5 40.5 52-53 34.95940001895152 38.5 34.5 39.5 26.5 40.5 54-55 35.4511605581923 39.0 35.0 40.5 27.0 41.0 56-57 35.35778869433547 39.0 35.0 41.0 27.0 41.0 58-59 35.13879701074704 38.5 35.0 40.5 26.0 41.0 60-61 34.830114542241226 38.0 34.5 40.0 26.0 41.0 62-63 34.453517574357704 38.0 34.0 40.0 25.5 41.0 64-65 34.07144453849544 37.0 34.0 40.0 24.5 41.0 66-67 33.658983244437124 37.0 33.5 39.5 23.0 41.0 68-69 33.215057493705615 36.0 33.0 39.0 22.0 41.0 70-71 32.76293520932211 35.5 32.5 39.0 21.5 40.0 72-73 32.31255576549208 35.0 32.0 38.0 20.0 40.0 74-75 31.83097435630008 35.0 31.5 37.0 18.0 39.5 76-77 31.385325002718716 35.0 31.0 37.0 16.0 39.0 78-79 30.969646401468296 35.0 31.0 36.0 11.5 39.0 80-81 30.50081895381234 34.5 31.0 36.0 7.5 37.5 82-83 30.020325508304644 34.0 30.0 35.5 4.5 37.0 84-85 29.595696764186457 34.0 30.0 35.0 2.0 37.0 86-87 29.136181636991846 34.0 29.0 35.0 2.0 36.0 88-89 28.65529993964767 34.0 29.0 35.0 2.0 36.0 90-91 28.192347941056948 34.0 28.5 35.0 2.0 35.5 92-93 27.679919748567983 34.0 27.0 35.0 2.0 35.0 94-95 27.132916633013934 33.0 26.0 35.0 2.0 35.0 96-97 26.471985769822943 33.0 25.0 35.0 2.0 35.0 98-99 25.588721586229745 33.0 22.0 35.0 2.0 35.0 100-101 22.27961175452309 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1439470894442927 1101 2 0.2718044980994634 1101 3 0.35417304311073394 1101 4 0.2705042798902966 1101 5 0.11262025720805013 1101 6 0.24346443052927214 1101 7 0.3312235260459957 1101 8 0.34483582332416773 1101 9 0.399603958388731 1101 10-11 0.34076598549108184 1101 12-13 0.2939184819154903 1101 14-15 0.5097642542235974 1101 16-17 0.4528902823459191 1101 18-19 0.5023655110610363 1101 20-21 0.41537252734845254 1101 22-23 0.49997383217655766 1101 24-25 0.48842933256943155 1101 26-27 0.5606790445621712 1101 28-29 0.49372010858527204 1101 30-31 0.4191515436756674 1101 32-33 0.4655308102806188 1101 34-35 0.3378045391482303 1101 36-37 0.3972182286340953 1101 38-39 0.4821891429325049 1101 40-41 0.4051832393006123 1101 42-43 0.47520464009829055 1101 44-45 0.4549771553449773 1101 46-47 0.496185828674804 1101 48-49 0.39724565951613755 1101 50-51 0.3809095124470403 1101 52-53 0.47089800588609876 1101 54-55 0.44833214274594724 1101 56-57 0.5462327449151445 1101 58-59 0.4020157380572016 1101 60-61 0.3914600295203243 1101 62-63 0.4793295035989118 1101 64-65 0.347159118663086 1101 66-67 0.3450246894607929 1101 68-69 0.27088318709895276 1101 70-71 0.29798420121772295 1101 72-73 0.2870082942176282 1101 74-75 0.02470787761068749 1101 76-77 -0.08242698982314778 1101 78-79 -0.015340189093070222 1101 80-81 -0.06422875854925181 1101 82-83 -0.04190120274619957 1101 84-85 0.044340588788639224 1101 86-87 0.09056270981250591 1101 88-89 -0.037703212396174024 1101 90-91 -0.15069026559532617 1101 92-93 -0.13144143801676478 1101 94-95 -0.2921030710688015 1101 96-97 -0.26448203947160565 1101 98-99 -0.32737129532973697 1101 100-101 0.18865883353296198 1102 1 0.2691669284901117 1102 2 0.28499333630414725 1102 3 0.33720940273862965 1102 4 0.1502333896391974 1102 5 0.25426049571247944 1102 6 0.22798196419145 1102 7 0.24729802066444506 1102 8 0.3354308145546767 1102 9 0.2883926334606528 1102 10-11 0.41893946630723633 1102 12-13 0.358419563883686 1102 14-15 0.5759291117923766 1102 16-17 0.5402938435428695 1102 18-19 0.5704958054282017 1102 20-21 0.44837857541725157 1102 22-23 0.4783688722345474 1102 24-25 0.4540927655350515 1102 26-27 0.528129324878897 1102 28-29 0.4740697527203537 1102 30-31 0.5108236484352986 1102 32-33 0.5178372571339835 1102 34-35 0.5236029618638796 1102 36-37 0.5110778028640155 1102 38-39 0.49680057823397306 1102 40-41 0.445305081336663 1102 42-43 0.2821474415242804 1102 44-45 0.3716456441823581 1102 46-47 0.4426156646373087 1102 48-49 0.4640648435434045 1102 50-51 0.404694206493879 1102 52-53 0.34471345282511834 1102 54-55 0.507725195400802 1102 56-57 0.41446036325910995 1102 58-59 0.28132417712825486 1102 60-61 0.42858712562682655 1102 62-63 0.44781335592324467 1102 64-65 0.38107431289878946 1102 66-67 0.4030729299760978 1102 68-69 0.4108666210595757 1102 70-71 0.42782966241782105 1102 72-73 0.3744600008426602 1102 74-75 0.3170607587233789 1102 76-77 0.38213862707926793 1102 78-79 0.3542985416570339 1102 80-81 0.4347154523341423 1102 82-83 0.5511296061150439 1102 84-85 0.433070135256699 1102 86-87 0.3339340873956367 1102 88-89 0.2187325273994496 1102 90-91 0.4326304128459739 1102 92-93 0.48563956686658827 1102 94-95 0.5444407431765086 1102 96-97 0.34685095296535096 1102 98-99 0.2986751906859233 1102 100-101 0.330215971022465 1103 1 0.19817102027968403 1103 2 0.1931151986383597 1103 3 0.2905368542463549 1103 4 0.2575040080237869 1103 5 0.13003763007170477 1103 6 0.23119359834297626 1103 7 0.19592882164521086 1103 8 0.10521302627579132 1103 9 0.31353108266413443 1103 10-11 0.3273831780229983 1103 12-13 0.3464322506304711 1103 14-15 0.3657622222202548 1103 16-17 0.29851434381009057 1103 18-19 0.4196598645819094 1103 20-21 0.41047817531726594 1103 22-23 0.31266158337753325 1103 24-25 0.3786908850590436 1103 26-27 0.4579604096883543 1103 28-29 0.38833444415349305 1103 30-31 0.3661498042735545 1103 32-33 0.3697416007337466 1103 34-35 0.24197471570443696 1103 36-37 0.20511494915502482 1103 38-39 0.40537538014604024 1103 40-41 0.26904384636714695 1103 42-43 0.2940654519957562 1103 44-45 0.42143792750424325 1103 46-47 0.4439025073377536 1103 48-49 0.3833353243312061 1103 50-51 0.46519225643263695 1103 52-53 0.39286748698341967 1103 54-55 0.433462927043081 1103 56-57 0.41178514474350436 1103 58-59 0.3886117429179876 1103 60-61 0.3978588068558153 1103 62-63 0.3523700592171579 1103 64-65 0.2776819770399612 1103 66-67 0.3131177175292734 1103 68-69 0.2944339126483584 1103 70-71 0.3645987425227588 1103 72-73 0.273204752947521 1103 74-75 0.23092385718114627 1103 76-77 0.2143467569512687 1103 78-79 0.19595157137894503 1103 80-81 0.3010755794477511 1103 82-83 0.5264386491092026 1103 84-85 0.45659247788844226 1103 86-87 0.4545770658940178 1103 88-89 0.4360647899526846 1103 90-91 0.4900206819283959 1103 92-93 0.4538920836066893 1103 94-95 0.25715675449979614 1103 96-97 0.32658639590091454 1103 98-99 0.4480894157325288 1103 100-101 0.504416381351426 1104 1 0.15176576318527069 1104 2 0.1998025473567182 1104 3 0.2191749239411962 1104 4 0.2079356865884492 1104 5 0.1626872539793851 1104 6 0.21910525062136088 1104 7 0.15604634741571033 1104 8 0.14562742240812554 1104 9 0.2268259012840943 1104 10-11 0.2812782334930546 1104 12-13 0.287488202115334 1104 14-15 0.402187002900547 1104 16-17 0.3733843131471275 1104 18-19 0.366926014341459 1104 20-21 0.36385262530166074 1104 22-23 0.3799830497960883 1104 24-25 0.40864951525553295 1104 26-27 0.4107456012561812 1104 28-29 0.4332194286158071 1104 30-31 0.5143700235625843 1104 32-33 0.5118498985444262 1104 34-35 0.4760766835640098 1104 36-37 0.5582215169118498 1104 38-39 0.6153055814907518 1104 40-41 0.5899721567134009 1104 42-43 0.547843852807965 1104 44-45 0.6168106394881363 1104 46-47 0.652430830921702 1104 48-49 0.6015952637415936 1104 50-51 0.5325271516662511 1104 52-53 0.5378088121147471 1104 54-55 0.7139637142130582 1104 56-57 0.6977263623437224 1104 58-59 0.6234520944250193 1104 60-61 0.6962476307018548 1104 62-63 0.6899254052988439 1104 64-65 0.6706342532442306 1104 66-67 0.676980414081612 1104 68-69 0.6814104667419869 1104 70-71 0.6449168072766085 1104 72-73 0.5902852299752439 1104 74-75 0.48416490421026026 1104 76-77 0.5458879051038892 1104 78-79 0.6451231543163658 1104 80-81 0.6025730985370394 1104 82-83 0.7366391367914567 1104 84-85 0.6120608372362888 1104 86-87 0.6730934604653953 1104 88-89 0.553119395135969 1104 90-91 0.5282272702157158 1104 92-93 0.6318914295680713 1104 94-95 0.6114054253740235 1104 96-97 0.558322327255631 1104 98-99 0.6752888720616745 1104 100-101 0.788250431776774 1105 1 0.09349396014605205 1105 2 0.180388504397186 1105 3 0.20113182720212563 1105 4 0.16491730741926602 1105 5 0.14340065902493393 1105 6 0.16310578415271237 1105 7 0.21310985887079426 1105 8 0.22611662156690926 1105 9 0.20949035724255793 1105 10-11 0.2793399072625746 1105 12-13 0.2721401729976378 1105 14-15 0.2434268738270049 1105 16-17 0.2491984036255559 1105 18-19 0.3020970265290899 1105 20-21 0.26857553452821037 1105 22-23 0.10569371828049157 1105 24-25 0.22867497669123793 1105 26-27 0.1811548099428819 1105 28-29 0.19361602226358343 1105 30-31 0.21549756119369334 1105 32-33 0.19124971400132296 1105 34-35 0.38546819327364545 1105 36-37 0.40221962621516383 1105 38-39 0.49843933178460276 1105 40-41 0.4271730223137027 1105 42-43 0.3031332216425895 1105 44-45 0.49063932744060423 1105 46-47 0.4822416737392814 1105 48-49 0.4192882355625116 1105 50-51 0.35447001653444943 1105 52-53 0.2487376747021557 1105 54-55 0.44046260887572686 1105 56-57 0.2921542188765045 1105 58-59 0.2748669131375223 1105 60-61 0.44272040405594737 1105 62-63 0.33502993831356065 1105 64-65 0.4367656550545931 1105 66-67 0.4378393210927527 1105 68-69 0.36315806155068486 1105 70-71 0.273284711342356 1105 72-73 0.22357064540695148 1105 74-75 0.23092385718113917 1105 76-77 0.14594077540497707 1105 78-79 0.19245173046262565 1105 80-81 0.2730768521171747 1105 82-83 0.32631138216678934 1105 84-85 0.33871147247959854 1105 86-87 0.518210537099872 1105 88-89 0.4850625627811951 1105 90-91 0.4101606755650522 1105 92-93 0.44959682430029346 1105 94-95 0.4848054977387406 1105 96-97 0.2411584608070605 1105 98-99 0.3244813979151573 1105 100-101 0.588571647021169 1106 1 0.17726538815559323 1106 2 0.1627818393178444 1106 3 0.19460342637587758 1106 4 0.20979084467469988 1106 5 0.22987334895785239 1106 6 0.21847533237613703 1106 7 0.17971703596780486 1106 8 0.16997588624211346 1106 9 0.23451882022733628 1106 10-11 0.20932517885318447 1106 12-13 0.3165627975763172 1106 14-15 0.30514923382809656 1106 16-17 0.27679854381585045 1106 18-19 0.3913672011840035 1106 20-21 0.280624103863758 1106 22-23 0.35013017927619217 1106 24-25 0.2860585313018902 1106 26-27 0.40924498404996257 1106 28-29 0.2246194293934849 1106 30-31 0.2503972893116071 1106 32-33 0.3695157932172677 1106 34-35 0.24233748267880628 1106 36-37 0.21912519934132746 1106 38-39 0.35060178115350027 1106 40-41 0.3670049765706409 1106 42-43 0.22237876466576978 1106 44-45 0.30089218269412754 1106 46-47 0.20290759169424888 1106 48-49 0.3568133008991978 1106 50-51 0.165467369353955 1106 52-53 0.2325487156010695 1106 54-55 0.2334041398296165 1106 56-57 0.19677565239953765 1106 58-59 0.24055751540093695 1106 60-61 0.3722912854919045 1106 62-63 0.332553781550466 1106 64-65 0.1080418854246119 1106 66-67 0.14741503473931772 1106 68-69 0.1609774562741606 1106 70-71 0.19856419643099343 1106 72-73 4.5034373091823454E-4 1106 74-75 0.1173640982578874 1106 76-77 -0.008696285543390303 1106 78-79 0.14020310198868557 1106 80-81 0.19829769046357626 1106 82-83 0.26592455246920466 1106 84-85 0.36269307355673064 1106 86-87 0.5374954823055553 1106 88-89 0.6525710033467611 1106 90-91 0.611397665294497 1106 92-93 0.6590376855575535 1106 94-95 0.7760101018240633 1106 96-97 0.9531625709955058 1106 98-99 1.081670189153126 1106 100-101 1.2017591571614332 1107 1 0.1111688750862676 1107 2 0.22089622193971792 1107 3 0.17987649975748354 1107 4 0.1654479547115173 1107 5 0.08075594788161311 1107 6 0.07890988106727548 1107 7 0.1066399302067893 1107 8 0.14266073134680823 1107 9 0.16578931239382655 1107 10-11 0.23246813935616473 1107 12-13 0.2820722788767469 1107 14-15 0.2584852716519279 1107 16-17 0.1974649139444935 1107 18-19 0.2730679473186868 1107 20-21 0.21159218300933702 1107 22-23 0.19176652746919132 1107 24-25 0.22334228284046986 1107 26-27 0.34573795093081117 1107 28-29 0.3055006730847012 1107 30-31 0.30975089815844115 1107 32-33 0.2006182206169811 1107 34-35 0.38455678997275555 1107 36-37 0.34962119752187704 1107 38-39 0.4596573732701259 1107 40-41 0.3327051961462004 1107 42-43 0.3682496320155977 1107 44-45 0.1466847432939744 1107 46-47 0.32650121431446166 1107 48-49 0.5041523202346099 1107 50-51 0.4518598687940809 1107 52-53 0.5562837464303669 1107 54-55 0.5288746061660277 1107 56-57 0.5850780040814882 1107 58-59 0.6209009876859284 1107 60-61 0.6122145440803237 1107 62-63 0.5078563464251289 1107 64-65 0.5814362111924467 1107 66-67 0.43704485710428287 1107 68-69 0.5817431039075203 1107 70-71 0.33825685637805236 1107 72-73 0.5305494509602866 1107 74-75 0.557479499146325 1107 76-77 0.6045259744846128 1107 78-79 0.5555902389499181 1107 80-81 0.4456489875485694 1107 82-83 0.5891595114542518 1107 84-85 0.5121642192022051 1107 86-87 0.604182631803436 1107 88-89 0.5875938267173417 1107 90-91 0.5443676748249402 1107 92-93 0.6043544509988692 1107 94-95 0.7381754646893199 1107 96-97 0.7178642301446665 1107 98-99 0.7480130836968151 1107 100-101 0.8454395963165204 1108 1 0.19783439199764885 1108 2 0.17883933361763837 1108 3 0.1483266688687479 1108 4 0.15263377853591464 1108 5 0.21934550520365548 1108 6 0.22233874814720167 1108 7 0.22271325320597413 1108 8 0.2549345395973077 1108 9 0.3243643459525174 1108 10-11 0.32020548748484146 1108 12-13 0.3110598272317091 1108 14-15 0.39019866120044355 1108 16-17 0.3532599620474173 1108 18-19 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-2.662885974526862 2316 98-99 -2.6706273086370835 2316 100-101 -2.6440145752494786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8690.0 3 2497.0 4 2465.0 5 2761.0 6 3158.0 7 3866.0 8 4520.0 9 5559.0 10 7019.0 11 8795.0 12 11795.0 13 17599.0 14 15577.0 15 15867.0 16 20213.0 17 26596.0 18 23695.0 19 17639.0 20 17498.0 21 18666.0 22 20112.0 23 22491.0 24 25463.0 25 29220.0 26 34081.0 27 39940.0 28 47965.0 29 57676.0 30 70414.0 31 87004.0 32 110799.0 33 143224.0 34 196178.0 35 285176.0 36 430722.0 37 650113.0 38 573483.0 39 86303.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04259303672482 19.113127707671033 14.699113644365747 23.145165611238397 2 14.920108071296506 20.291658843484374 41.2014498610921 23.58678322412701 3 19.395349754973882 24.39080822656339 29.957060755911463 26.256781262551264 4 12.78335671564646 16.806143649728707 37.444079776548264 32.96641985807657 5 13.80536595939864 37.20018245369665 34.33525834927787 14.659193237626841 6 31.618232813362958 36.04240316410754 17.717774180658527 14.621589841870977 7 27.97060210268585 32.88457879790712 21.046997058428655 18.097822040978386 8 25.36212541506545 35.63793216994641 20.008564577454862 18.991377837533275 9 25.93438653815256 17.469635917349247 20.801214086070942 35.794763458427255 10-11 24.66836813419832 26.720325887663055 27.549011841234115 21.062294136904505 12-13 25.7924439190781 24.11592186124761 27.971781018950885 22.1198532007234 14-15 22.578360188435088 25.444383732481352 26.61525503398942 25.36200104509414 16-17 21.932440461277462 28.697397818007126 27.146937343860728 22.223224376854684 18-19 21.806188355074767 27.491775373846643 29.303547566019727 21.39848870505886 20-21 22.58879138340105 26.587318884731797 29.231564415294258 21.592325316572897 22-23 22.170897532728905 26.569595966040637 29.19700921600989 22.062497285220573 24-25 22.170955631388455 26.684210141078683 29.244875312232583 21.89995891530028 26-27 22.097241929945767 26.67758383503832 29.29886575797045 21.926308477045463 28-29 21.78548863474904 27.061677574399074 29.322874129811797 21.82995966104009 30-31 22.045924435092857 26.80746023475318 29.134308886175337 22.012306443978634 32-33 21.81723653640194 26.965616271944032 29.166494319544928 22.050652872109104 34-35 22.27729158657016 26.897930356823647 29.025633698560107 21.799144358046085 36-37 22.186950481894783 26.720350560705135 29.10475499229387 21.98794396510621 38-39 22.041439701547024 26.97251858344702 29.14660902094629 21.839432694059667 40-41 22.19415907636539 26.9707161897901 29.000954136315045 21.834170597529464 42-43 21.990009673467373 26.972412331506867 28.79693975379852 22.24063824122724 44-45 22.129184806966133 26.970228985443356 28.68679724807305 22.213788959517462 46-47 22.202396581386598 27.200869730207312 28.403089350181084 22.193644338225006 48-49 22.35932175665461 27.1882785329221 28.32235280436384 22.13004690605945 50-51 22.111295568824513 27.38377606645461 28.378537896902 22.126390467818872 52-53 22.29348096938873 27.52025416743871 28.005539546741126 22.18072531643143 54-55 22.101557838192846 27.22631139130846 28.223263308131756 22.448867462366938 56-57 22.242962000110182 27.49164637977853 28.05200363127979 22.2133879888315 58-59 22.407791482058038 27.266307428252105 28.08939448369457 22.23650660599529 60-61 22.28770089885284 27.11385389519656 28.48692706091448 22.11151814503612 62-63 22.13968141110131 27.28681034038063 28.416930059958005 22.15657818856005 64-65 22.038149611267567 27.371092108899393 28.274912980484306 22.315845299348734 66-67 22.064548884060013 27.359868080311 28.06108282906515 22.51450020656384 68-69 21.9299389272777 27.373358462879594 28.24774779956694 22.448954810275758 70-71 22.1515982032815 27.253718905007634 28.068661820498786 22.52602107121208 72-73 22.118843746540332 27.088258077832382 28.22246231447189 22.570435861155396 74-75 21.988016046554243 27.52887314189532 27.951747868891903 22.53136294265854 76-77 22.15647181279948 27.2136738704093 27.964969459600315 22.66488485719091 78-79 22.090885471518025 27.256746802116638 28.061720909413623 22.59064681695171 80-81 22.090901890041827 27.375743228223126 27.968984354838298 22.56437052689675 82-83 22.109329245144252 27.30052869305346 28.080543798569273 22.50959826323302 84-85 22.133185971799538 27.229504675188398 27.904881537138383 22.732427815873677 86-87 22.05675409216595 27.12101158658443 28.1075969452316 22.714637376018022 88-89 22.097239372424774 27.05187898337912 27.929825603802925 22.92105604039318 90-91 22.06656706170529 27.010926247866834 28.0338392760584 22.888667414369472 92-93 22.15542479362502 27.088966570719915 28.00594019990025 22.749668435754813 94-95 22.084605104552175 26.978031941194843 27.99785599542442 22.939506958828563 96-97 21.93808418493272 26.902084574722156 28.08576645542422 23.0740647849209 98-99 21.939624488627434 26.898884022426905 27.85196043724032 23.309531051705342 100-101 22.226103428558652 26.168578520158313 28.454625024357366 23.15069302692567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3684.0 1 2268.0 2 969.5 3 1058.0 4 882.0 5 897.0 6 1603.0 7 3256.0 8 5252.0 9 5985.5 10 5008.0 11 4043.5 12 3989.0 13 4273.0 14 4668.0 15 4929.5 16 5177.5 17 5410.5 18 5527.0 19 5726.5 20 6152.0 21 7012.0 22 8274.5 23 9889.0 24 12061.5 25 15072.5 26 18663.0 27 22863.0 28 27381.5 29 31711.0 30 36387.5 31 42156.0 32 47558.0 33 52723.5 34 58880.0 35 64731.0 36 71124.0 37 78097.0 38 83900.5 39 87245.5 40 90526.0 41 95895.0 42 100565.0 43 105200.0 44 110274.5 45 113769.0 46 115506.0 47 120923.5 48 136065.5 49 137432.5 50 124601.0 51 117876.0 52 111874.0 53 107493.5 54 102893.5 55 95120.5 56 86616.5 57 77902.5 58 69141.0 59 59921.0 60 50289.0 61 40267.0 62 31421.0 63 24408.5 64 18473.0 65 13614.5 66 9589.5 67 7104.5 68 5205.0 69 3431.0 70 2262.5 71 1511.5 72 989.0 73 617.5 74 419.5 75 296.5 76 185.5 77 116.0 78 85.0 79 59.0 80 46.5 81 43.0 82 30.0 83 23.5 84 24.0 85 16.5 86 11.5 87 10.0 88 11.5 89 13.0 90 9.5 91 4.0 92 5.5 93 6.5 94 6.5 95 5.0 96 3.5 97 2.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29667400773196695 2 0.07936745158617252 3 0.08750770303090816 4 0.09189580732533596 5 0.10324764234787746 6 0.05119455010165775 7 0.05240286867548569 8 0.053642985106519644 9 0.05599602653976354 10-11 0.05396096367857963 12-13 0.052863937604972676 14-15 0.05114685331584875 16-17 0.05210078903202871 18-19 0.05933480154639339 20-21 0.060702109406251335 22-23 0.07458187407666972 24-25 0.0819430780198584 26-27 0.07679182515248663 28-29 0.07537682050681968 30-31 0.07006657835341792 32-33 0.06308694869670123 34-35 0.29653091737453996 36-37 0.4631834869911786 38-39 0.43838115837049973 40-41 0.42094003369300953 42-43 0.44954220624980523 44-45 0.4580481330524099 46-47 0.4528014866134201 48-49 0.46997232950465934 50-51 0.4664586662833965 52-53 0.4657114166390555 54-55 0.45706239947902394 56-57 0.4368548612246118 58-59 0.4210354272646275 60-61 0.3828303018316202 62-63 0.44807950481832937 64-65 0.47127604165010534 66-67 0.4796070802380769 68-69 0.49229442526327033 70-71 0.4891464373998765 72-73 0.47857364987888196 74-75 0.4800681491675639 76-77 0.4559971712626229 78-79 0.43790419051240975 80-81 0.44294415087956057 82-83 0.4522132262551091 84-85 0.4529127791136411 86-87 0.4521496305406971 88-89 0.4590497655543988 90-91 0.47170531272238625 92-93 0.4771268473760091 94-95 0.48359771131742973 96-97 0.47604572023100505 98-99 0.4786849423791029 100-101 0.4577619523375559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3144866.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.878128704514 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33984934127577 42.851896474117765 2 13.787519487378471 15.684166158382151 3 4.699146007313748 8.01835894215883 4 2.1074751021969864 4.794769604172759 5 1.1713731434800323 3.331275620793421 6 0.709048523479293 2.419761190572051 7 0.47769975989299307 1.9019467897716362 8 0.33042262013698515 1.5035056252027341 9 0.2309656429904347 1.18232042114977 >10 1.0555686834198041 10.314676849241481 >50 0.05992210550804304 2.360761805444805 >100 0.027711060395356833 2.8375535805862913 >500 0.002124472140386838 0.7817771197655323 >1k 9.504217457274856E-4 0.9066197859178038 >5k 1.6772148454014451E-4 0.7062864180498873 >10k+ 5.5907161513381505E-5 0.4043236146731042 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12715 0.40430975437427225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9848 0.31314529776467426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6635 0.21097878256180072 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5728 0.18213812607595997 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3331 0.10591866235318134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.17978572059986E-5 0.0 5 0.0 0.0 0.0 3.17978572059986E-5 0.0 6 0.0 0.0 0.0 6.35957144119972E-5 0.0 7 0.0 0.0 0.0 6.35957144119972E-5 0.0 8 0.0 0.0 0.0 6.35957144119972E-5 0.0 9 0.0 0.0 0.0 9.53935716179958E-5 0.0 10-11 0.0 0.0 0.0 9.53935716179958E-5 0.0 12-13 0.0 0.0 0.0 9.53935716179958E-5 0.0 14-15 0.0 0.0 0.0 1.271914288239944E-4 0.0 16-17 0.0 0.0 0.0 1.58989286029993E-4 0.0 18-19 0.0 0.0 0.0 1.907871432359916E-4 0.0 20-21 0.0 3.17978572059986E-5 0.0 3.17978572059986E-4 0.0 22-23 0.0 3.17978572059986E-5 0.0 5.087657152959776E-4 0.0 24-25 0.0 3.17978572059986E-5 0.0 6.995528585319692E-4 0.0 26-27 0.0 3.17978572059986E-5 0.0 8.903400017679609E-4 0.0 28-29 0.0 3.17978572059986E-5 0.0 0.0019078714323599163 0.0 30-31 0.0 3.17978572059986E-5 0.0 0.005548726082446756 0.0 32-33 0.0 3.17978572059986E-5 0.0 0.013243807526298418 0.0 34-35 0.0 3.17978572059986E-5 0.0 0.025120307192738896 0.0 36-37 0.0 3.17978572059986E-5 0.0 0.044024133301705064 0.0 38-39 0.0 3.17978572059986E-5 0.0 0.07119540228423088 0.0 40-41 0.0 3.17978572059986E-5 0.0 0.10275477556118448 0.0 42-43 0.0 3.17978572059986E-5 0.0 0.13690567420042699 0.0 44-45 0.0 3.17978572059986E-5 0.0 0.1795624996422741 0.0 46-47 0.0 3.17978572059986E-5 0.0 0.23234694260423178 0.0 48-49 0.0 3.17978572059986E-5 0.0 0.29033033521937024 0.0 50-51 0.0 3.17978572059986E-5 0.0 0.35298801284379044 0.0 52-53 0.0 3.17978572059986E-5 0.0 0.4197158161905785 0.0 54-55 0.0 3.17978572059986E-5 0.0 0.49703230598696413 0.0 56-57 0.0 3.17978572059986E-5 0.0 0.5907246922444391 0.0 58-59 0.0 3.17978572059986E-5 0.0 0.6948308767368785 0.0 60-61 0.0 3.17978572059986E-5 0.0 0.8113382255396573 0.0 62-63 0.0 3.17978572059986E-5 0.0 0.9362084107876139 0.0 64-65 0.0 4.76967858089979E-5 0.0 1.068996262479864 1.58989286029993E-5 66-67 0.0 6.35957144119972E-5 0.0 1.224201603502343 3.17978572059986E-5 68-69 0.0 6.35957144119972E-5 0.0 1.4062761338638912 3.17978572059986E-5 70-71 0.0 6.35957144119972E-5 0.0 1.6042495928284386 3.17978572059986E-5 72-73 0.0 6.35957144119972E-5 0.0 1.8121916800270663 3.17978572059986E-5 74-75 0.0 6.35957144119972E-5 0.0 2.0349833665408954 3.17978572059986E-5 76-77 0.0 6.35957144119972E-5 0.0 2.275470560589863 3.17978572059986E-5 78-79 0.0 6.35957144119972E-5 0.0 2.5567862032913324 3.17978572059986E-5 80-81 0.0 6.35957144119972E-5 0.0 2.871839372488367 3.17978572059986E-5 82-83 0.0 6.35957144119972E-5 0.0 3.1940947563425595 3.17978572059986E-5 84-85 0.0 6.35957144119972E-5 0.0 3.5336322755882126 3.17978572059986E-5 86-87 0.0 6.35957144119972E-5 0.0 3.890118052724663 3.17978572059986E-5 88-89 0.0 6.35957144119972E-5 0.0 4.286669129940671 3.17978572059986E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20065 0.0 23.466875 1 GTACATG 10400 0.0 23.276606 1 TACATGG 10295 0.0 22.409967 2 GGTATCA 14975 0.0 21.83912 1 GAGTACT 10055 0.0 21.545036 12-13 ACATGGG 10860 0.0 20.415249 3 ATCAACG 22895 0.0 20.38141 3 TCAACGC 23020 0.0 20.311895 4 CAACGCA 22955 0.0 20.28686 5 TATCAAC 23370 0.0 20.007694 2 AACGCAG 23470 0.0 19.812378 6 GTACTTT 10865 0.0 19.634377 14-15 AGTACTT 10670 0.0 19.393457 12-13 AGAGTAC 17275 0.0 18.488518 10-11 CATGGGG 5520 0.0 18.10848 4 ACGCAGA 25545 0.0 17.980595 7 CGCAGAG 25725 0.0 17.615492 8 GCAGAGT 26265 0.0 16.65838 9 TACTTTT 12510 0.0 16.42799 14-15 ACTTTTT 13265 0.0 16.423334 16-17 >>END_MODULE